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https://www.readbyqxmd.com/read/28342908/intestinal-dendritic-cell-licensing-through-tlr4-is-required-for-oral-tolerance-in-allergic-contact-dermatitis
#1
Feriel Hacini-Rachinel, Mercedes Gomez de Agüero, Reem Kanjarawi, Ludovic Moro-Sibilot, Jean-Benoit Le Luduec, Claire Macari, Gilles Boschetti, Emilie Bardel, Philippe Langella, Bertrand Dubois, Dominique Kaiserlian
BACKGROUND: Induction of oral tolerance to haptens is an efficient way to prevent allergic contact dermatitis (ACD) in mice. Toll-like receptor (TLRs)-mediated sensing of the microbiota contributes to gut homeostasis, yet whether it contributes to induction of oral tolerance has not been documented. OBJECTIVE: We examined whether oral tolerance to the contact sensitizer DNFB depends on microbiota/TLRs and evaluated the role of TLR4 on the tolerogenic function of intestinal dendritic cells (DCs)...
March 22, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28326907/skin-sensitizers-in-cosmetics-and-beyond-potential-multiple-mechanisms-of-action-and-importance-of-t-cell-assays-for-in-vitro-screening
#2
Stanislav Vukmanović, Nakissa Sadrieh
Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity (DTH) reaction induced by repeated contact with sensitizers. The ability of a chemical to act as a sensitizer has most frequently been tested in animals. As the use of animals for these purposes is gradually and globally being phased out, there is a need for reliable in vitro surrogate assays. Currently proposed in vitro assays are designed to test four key events of the adverse outcome pathway (AOP) involving covalent modification of self-proteins by sensitizers (haptenation) and presentation of new antigens (hapten/carrier complexes) to the immune system...
March 22, 2017: Critical Reviews in Toxicology
https://www.readbyqxmd.com/read/28325474/corrigendum-to-epicutaneous-immunization-with-hapten-conjugated-protein-antigen-alleviates-contact-sensitivity-mediated-by-three-different-types-of-effector-cells-pharmacol-rep-64-2012-919-926
#3
Monika Majewska-Szczepanik, Anna Strzępa, Iwona Drożyńska, Sylwia Motyl, Tomasz Banach, Marian Szczepanik
No abstract text is available yet for this article.
March 16, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28320580/roles-of-alternatively-activated-m2-macrophages-in-allergic-contact-dermatitis
#4
REVIEW
Kotaro Suzuki, Kazuyuki Meguro, Daiki Nakagomi, Hiroshi Nakajima
Alternatively activated macrophages (M2 macrophages) play key roles in the suppression of Th1 cell responses and the orchestration of tissue repair. However, recent studies have shown that M2 macrophages have potentials to produce high levels of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α, suggesting that M2 macrophages may exacerbate inflammation in some settings. In this regard, we have recently shown that large numbers of M2 macrophages accumulate in the sites of hapten-induced contact hypersensitivity (CHS), an animal model of allergic contact dermatitis, and that M2 macrophages exacerbate hapten-induced CHS by producing matrix metalloproteinase 12 (MMP12)...
March 17, 2017: Allergology International: Official Journal of the Japanese Society of Allergology
https://www.readbyqxmd.com/read/28304015/sodium-periodate-mediated-conjugation-of-harringtonine-enabling-the-production-of-a-highly-specific-monoclonal-antibody-and-the-development-of-a-sensitive-quantitative-analysis-method
#5
Seiichi Sakamoto, Gorawit Yusakul, Yumi Tsuneura, Waraporn Putalun, Kazuteru Usui, Tomofumi Miyamoto, Hiroyuki Tanaka, Satoshi Morimoto
Harringtonine (HT) is a promising natural product that is mainly isolated from plants of the genus Cephalotaxus. Due to its remarkable antileukemic activities, HT has been utilized clinically in China for the treatment of acute promyelocytic leukemia (APL). No antibody that recognizes free HT has been reported to date due to the difficulty of preparing antigen conjugates in which haptens bind to a carrier protein. To overcome this difficulty, we focused on sodium periodate (NaIO4), which catalyzes unique oxidative reactions; the resulting conjugates enabled the production of a highly specific monoclonal antibody (MAb) against HT (MAb 1D2) and the establishment of an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the determination of HT...
March 17, 2017: Analyst
https://www.readbyqxmd.com/read/28295200/isothiocyanates-are-important-as-haptens-in-contact-allergy-to-chloroprene-rubber
#6
A G Ramzy, K Lammintausta, M Matura, J B Christensson, U Nilsson, L Hagvall
BACKGROUND: Contact allergy to chloroprene rubber products is well known. Thiourea compounds are considered the cause of allergy, despite being non- or weak sensitizers. Diethylthiourea is commonly occurring in this type of products and can decompose to the sensitizer ethyl isothiocyanate. OBJECTIVES: To investigate the clinical importance of degradation products and metabolites from organic thioureas in contact allergy to chloroprene rubber with focus on isothiocyanates and isocyanates...
March 10, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28294277/the-design-and-synthesis-of-an-%C3%AE-gal-trisaccharide-epitope-that-provides-a-highly-specific-anti-gal-immune-response
#7
Kensaku Anraku, Shun Sato, Nicholas T Jacob, Lisa M Eubanks, Beverly A Ellis, Kim D Janda
Carbohydrate antigens displaying Galα(1,3)Gal epitopes are recognized by naturally occurring antibodies in humans. These anti-Gal antibodies comprise up to 1% of serum IgG and have been viewed as detrimental as they are responsible for hyperacute organ rejections. In order to model this condition, α(1,3)galactosyltransferase-knockout mice are inoculated against the Galα(1,3)Gal epitope. In our study, two α-Gal trisaccharide epitopes composed of either Galα(1,3)Galβ(1,4)GlcNAc or Galα(1,3)Galβ(1,4)Glc linked to a squaric acid ester moiety were examined for their ability to elicit immune responses in KO mice...
March 15, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28288463/guide-to-treatment-of-tattoo-complications-and-tattoo-removal
#8
Jørgen Serup, Wolfgang Bäumler
Clinicians in the fields of general medicine, dermatology, and plastic surgery are in their work now and then confronted with tattoo complications. Recognizing the rather few important diagnostic groups and urgencies, the medical 'decision tree' of treatment becomes quite simple. Acute conditions are dominated by bacterial infections needing antibiotic treatment. Systemic infection is a matter of urgency and requires intravenous treatment in a hospital without delay to prevent septic shock. Inflammatory reactions are a real challenge...
2017: Current Problems in Dermatology
https://www.readbyqxmd.com/read/28288456/from-technique-of-tattooing-to-biokinetics-and-toxicology-of-injected-tattoo-ink-particles-and-chemicals
#9
Jørgen Serup
Tattoo colourants are colourful nano- and microparticles, which are practically insoluble and thus permanent once installed in the dermis by the tattooist. Tattoo ink also has soluble ingredients and contaminants. Pigments can distribute via the lymph and possibly also directly to the blood, and a minute fraction may over time undergo metabolic breakdown and as hapten(s) induce allergic reactions of red tattoos. Carbon black of black tattoos has a tendency to agglomerate and form larger bodies that can elicit foreign body reactions in black tattoos and even granuloma formation with overlap to sarcoidosis in the clinic...
2017: Current Problems in Dermatology
https://www.readbyqxmd.com/read/28288451/how-to-diagnose-and-classify-tattoo-complications-in-the-clinic-a-system-of-distinctive-patterns
#10
Jørgen Serup
Tattoo complications represent a broad spectrum of clinical entities and disease mechanisms. Infections are known, but chronic inflammatory reactions have hitherto been inconsistently reported and given many interpretations and terms. A clinical classification system of distinct patterns with emphasis on inflammatory tattoo reactions is introduced. Allergic reactions prevalent in red tattoos and often associated with azo pigments are manifested as the 'plaque elevation', 'excessive hyperkeratosis', and 'ulceronecrotic' patterns...
2017: Current Problems in Dermatology
https://www.readbyqxmd.com/read/28282494/hapten-specific-single-cell-selection-of-hybridoma-clones-by-facs-for-the-generation-of-monoclonal-antibodies
#11
Martin Dippong, Peter Carl, Christine Lenz, Jörg Schenk, Katrin Hoffmann, Timm Schwaar, Rudolf J Schneider, Maren Kuhne
The conventional hybridoma screening and subcloning process is generally considered to be one of the most critical steps in hapten-specific antibody production. It is time-consuming, monoclonality is not guaranteed and the number of clones that can be screened is limited. Our approach employs a novel hapten-specific labeling technique of hybridoma cells. This allows for fluorescence-activated cell sorting (FACS) and single-cell deposition and thereby eliminating the abovementioned problems. A two-step staining approach is used to detect antigen-specificity and antibody expression: in order to detect antigen-specificity hybridoma cells are incubated with a hapten-peroxidase conjugate (hapten-HRP), which is subsequently incubated with a fluorophore-labeled polyclonal anti-peroxidase antibody (anti-HRP-Alexa Fluor® 488)...
March 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28275634/exosomes-as-delivery-vehicles-a-commentary-on-amoxicillin-haptenates-intracellular-proteins-that-can-be-transported-in-exosomes-to-target-cells
#12
COMMENT
Stefania Raimondo
No abstract text is available yet for this article.
February 2017: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28274835/clinical-immunogenicity-of-the-d-amino-acid-peptide-therapeutic-etelcalcetide-method-development-challenges-and-anti-drug-antibody-clinical-impact-assessments
#13
Mark A Kroenke, Dohan Weeraratne, Hongjie Deng, Bethlyn Sloey, Raju Subramanian, Benjamin Wu, Michael Serenko, M Benjamin Hock
The immunogenicity risk assessment and bioanalytical strategy for novel therapeutics should account for both unique biophysical properties and potential consequences of immunogenicity. When assessing the immunogenicity risk of etelcalcetide, a peptide agonist of the calcium-sensing receptor, we considered the potential that the d-amino acid 'backbone' and biotransformation of etelcalcetide could allow the drug to act as a hapten. As a consequence, we validated and implemented a surface plasmon resonance immunoassay platform with both etelcalcetide and etelcalcetide-'carrier' surfaces to detect anti-drug antibodies (ADA)...
March 5, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28265582/glycomacropeptide-attenuates-inflammation-pruritus-and-th2-response-associated-with-atopic-dermatitis-induced-by-2-4-dinitrochlorobenzene-in-rat
#14
Fabiola Carolina Muñoz, Maritza Montserrat Cervantes, Daniel Cervantes-García, Mariela Jiménez, Javier Ventura-Juárez, Eva Salinas
Atopic dermatitis (AD) is one of the most common skin diseases, whose incidence is increasing in industrialized countries. The epicutaneous application of a hapten, such as 2,4-dinitrochlorobenzene (DNCB), evokes an experimental murine AD-like reaction. Glycomacropeptide (GMP) is a dairy bioactive peptide derived from hydrolysis of κ-casein by chymosin action. It has anti-inflammatory, prebiotic, and immunomodulatory effects. The present study was aimed to investigate the effect of GMP administration on DNCB-induced AD in rats...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28249223/pronounced-effect-of-hapten-binding-on-thermal-stability-of-an-anti-4-hydroxy-3-nitrophenyl-acetyl-antibody-possessing-a-glycine-residue-at-position-95-of-the-heavy-chain
#15
Yusui Sato, Satomi Inaba, Harumi Fukada, Takachika Azuma, Masayuki Oda
Immune response to T-cell-dependent antigens is highly dynamic; several B-cell clones responsible for antibody production appear alternately during immunization. It was previously shown that at least two-types of antibodies are secreted after immunization with (4-hydroxy-3-nitrophenyl)acetyl (NP); one has Tyr and another has Gly at position 95 of the heavy chain (referred to as Tyr95- and Gly95-type). The former appeared at an early stage, while the latter appeared at a late stage, i.e., after secondary immunization, although Fv domains of these antibodies were encoded by same genes of variable heavy and light chains...
February 26, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28215876/in-vitro-lectin-binding-to-the-outer-surface-of-spirocerca-lupi-at-different-life-stages
#16
I Aroch, I Arogeti, A Marcovics, Y Spiegel, E Lavy
Spirocerca lupi is the esophageal nematode of dogs. Early, transient eosinophilia occurs in experimentally infected dogs, but is absent in advanced cases, suggesting that the nematode evades the dog's immune system. Lectins are proteins or glycoproteins of plant or animal origin, binding different saccharides, with varying specificities and avidities, used to characterize surface haptens in plant and animal parasitic helminths. This study investigated the in vitro binding of six lectins (Concanavalin A [ConA], wheat germ agglutinin [WGA], peanut agglutinin [PNA], soybean agglutinin [SBA], Dolichus biflorus agglutinin [DBA] and Ulex earopaeus agglutinin I [UEA]) to the surface of S...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28185188/hapten-anti-hapten-technique-for-two-color-ihc-detection-of-phosphorylated-egfr-and-h2ax-using-primary-antibodies-raised-in-the-same-host-species
#17
Jodi Hagen, David Schwartz, Alexander E Kalyuzhny
Multiplex staining of cell and tissue sections with antibodies raised in the same host species is a serious challenge because of unwanted but inevitable cross-reactivity of secondary antibodies with irrelevant primary antibodies. Several techniques can be used to overcome this obstacle including direct labeling of primary antibodies with fluorescent tags and using tyramide signal amplification. Unfortunately these techniques either lack sensitivity, or require a long multistep protocol which can cause physical damage of specimens...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28174006/immunophotoaffinity-labeling-of-binders-of-1-methyladenine-the-oocyte-maturation-inducing-hormone-of-starfish
#18
Tetsuo Toraya, Tetsuo Kida, Atsushi Kuyama, Shinjiro Matsuda, Seiichi Tanaka, Yo Komatsu, Taro Tsurukai
Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary and resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the oocyte maturation-inducing hormone of starfish. Putative 1-MeAde receptors on the oocyte surface have been suggested, but not yet been biochemically characterized. Immunophotoaffinity labeling, i.e., photoaffinity labeling combined with immunochemical detection, was attempted to detect unknown 1-MeAde binders including putative maturation-inducing hormone receptors in starfish oocytes...
March 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28167389/engineering-of-a-hybrid-nanoparticle-based-nicotine-nanovaccine-as-a-next-generation-immunotherapeutic-strategy-against-nicotine-addiction-a-focus-on-hapten-density
#19
Zongmin Zhao, Kristen Powers, Yun Hu, Michael Raleigh, Paul Pentel, Chenming Zhang
Although vaccination is a promising way to combat nicotine addiction, most traditional hapten-protein conjugate nicotine vaccines only show limited efficacy due to their poor recognition and uptake by immune cells. This study aimed to develop a hybrid nanoparticle-based nicotine vaccine with improved efficacy. The focus was to study the impact of hapten density on the immunological efficacy of the proposed hybrid nanovaccine. It was shown that the nanovaccine nanoparticles were taken up by the dendritic cells more efficiently than the conjugate vaccine, regardless of the hapten density on the nanoparticles...
April 2017: Biomaterials
https://www.readbyqxmd.com/read/28158195/repeated-hapten-exposure-induces-persistent-tactile-sensitivity-in-mice-modeling-localized-provoked-vulvodynia
#20
Jasmine Landry, Tijana Martinov, Hanna Mengistu, Jyothi Dhanwada, Charles J Benck, Jaclyn Kline, Beebie Boo, Linnea Swanson, Elena Tonc, Randy Daughters, Brian T Fife, Devavani Chatterjea
BACKGROUND: Vulvodynia is a remarkably prevalent chronic pain condition of unknown etiology. Epidemiologic studies associate the risk of vulvodynia with a history of atopic disease. We used an established model of hapten-driven contact hypersensitivity to investigate the underlying mechanisms of allergy-provoked prolonged sensitivity to pressure. METHODS: We sensitized female ND4 Swiss mice to the hapten oxazolone on their flanks, and subsequently challenged them four days later with oxazolone or vehicle for ten consecutive days on the labia...
2017: PloS One
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