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https://www.readbyqxmd.com/read/28423239/a-novel-jak-inhibitor-jte-052-reduces-skin-inflammation-and-ameliorates-chronic-dermatitis-in-rodent-models-comparison-with-conventional-therapeutic-agents
#1
Atsuo Tanimoto, Yuichi Shinozaki, Yasuo Yamamoto, Yoshiaki Katsuda, Eriko Riya, Kaoru Toyoda, Kochi Kakimoto, Yukari Kimoto, Wataru Amano, Noriko Konishi, Mikio Hayashi
Janus kinases (JAKs) are required for several inflammatory cytokine signaling pathways and are implicated in the pathogenesis of chronic dermatitis, including atopic dermatitis and psoriasis. JAK inhibitors are therefore promising therapeutic candidates for chronic dermatitis. In this study, we evaluated the effects of the novel JAK inhibitor JTE-052 on inflammatory responses associated with chronic dermatitis, and compared its profile with those of conventional therapeutic agents in rodent models of chronic dermatitis...
April 19, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28412367/deciphering-antigen-responding-antibody-repertoires-by-using-next-generation-sequencing-and-confirming-them-through-antibody-gene-synthesis
#2
Naoko Kono, Lin Sun, Hiroyuki Toh, Takeyuki Shimizu, Hanbing Xue, Osamu Numata, Manabu Ato, Kazuo Ohnishi, Shigeyuki Itamura
Vast diversity and high specificity of antigen recognition by antibodies are hallmarks of the acquired immune system. Although the molecular mechanisms that yield the extremely large antibody repertoires are precisely understood, comprehensive description of the global antibody repertoire generated in individual bodies has been hindered by the lack of powerful measures. To obtain holistic understanding of the antibody-repertoire space, we used next-generation sequencing (NGS) to analyze the deep profiles of naive and antigen-responding repertoires of the IgM, IgG1, and IgG2c classes formed in individual mice...
April 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28411632/an-ultra-specific-and-sensitive-sandwich-elisa-for-imatinib-using-two-anti-imatinib-antibodies
#3
Tetsuya Saita, Yuta Yamamoto, Kazuhisa Hosoya, Yutaro Yamamoto, Sakiko Kimura, Yutaka Narisawa, Masashi Shin
The development of an immunoassay for a low-molecular-weight drug first requires the identification of specific antibodies that do not cross-react with the drug's metabolites. If two antibodies can simultaneously recognize the entire structure of the drug, we can then utilize them to establish an ultra-specific sandwich ELISA, free from interference due to the metabolic products of the drug. This paper reports an ultra-specific and sensitive sandwich ELISA for determination of the tyrosine kinase inhibitor imatinib using two anti-imatinib antibodies...
May 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28411454/synthesis-and-immunogenicity-of-pg-tb1-monovalent-glycoconjugate
#4
Xin Meng, Chuanming Ji, Chao Su, Di Shen, Yaxin Li, Peijie Dong, Ding Yuan, Mengya Yang, Song Bai, Demei Meng, Zhenchuan Fan, Yang Yang, Peng Yu, Tao Zhu
A PG-tb1 hapten from the West Beijing strains of Mycobacterium tuberculosis cell wall has been efficiently synthesized and conjugated to CRM197 in a simple way as linker-equipped carbohydrate by applying squaric acid chemistry for an original neoglycoprotein, creating a potent T-dependent conjugate vaccine. The intermediate monoester can be easily purified and the degree of incorporation can be monitored by MALDI-TOF mass spectrometry. After administered systemically in mice without any adjuvant, the conjugate induced high antigen-specific IgG levels in serum...
March 27, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28411375/development-of-tetramethylenedisulfotetramine-tets-hapten-library-synthesis-electrophysiological-studies-and-immune-response-in-rabbits
#5
Bogdan Barnych, Natalia Vasylieva, Tom Joseph, Susan Hulsizer, Hai Nguyen, Tomas Cajka, Isaac Pessah, Heike Wulff, Shirley Gee, Bruce Hammock
There is a need for fast detection methods for the banned rodenticide tetramethylenedisulfotetramine (TETS), a highly potent blocker of the γ-aminobutyric acid (GABAA) receptors. General synthetic approach toward two groups of analogues was developed. Screening of the resulting library of compounds by FLIPR or whole-cell voltage-clamp revealed that despite the structural differences some of the TETS analogues retained GABAA receptor inhibition, however their potency was an order of magnitude lower. Antibodies raised in rabbits against some of the TETS analogues conjugated to protein recognized free TETS and will be used for the development of an immunoassay for TETS...
April 14, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28405735/curcumin-inhibits-epigen-and-amphiregulin-upregulated-by-2-4-6-trinitrochlorobenzene-associated-with-attenuation-of-skin-swelling
#6
Hiroyasu Sakai, Ken Sato, Fumiaki Sato, Yuki Kai, Kazutaka Mandokoro, Kenjiro Matsumoto, Shinichi Kato, Tetsuro Yumoto, Minoru Narita, Yoshihiko Chiba
OBJECTIVES: Contact dermatitis model involving repeated application of hapten is used as a tool to assess dermatitis, as characterized by thickening. Involvement of cell proliferation, elicited by repeated hapten-stimulation, in this swelling has been unclear. Curcumin is reported to reduce inflammation. We examined involvement of cell proliferation and the role of extracellular regulated kinase (ERK) in 2,4,6-trinitrochlorobenzene (TNCB) challenge-induced ear swelling. We also examined the effects of curcumin in this model...
April 12, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28391867/ultrasensitive-immunoassay-for-monocrotaline-using-monoclonal-antibody-produced-by-n-n-carbonyldiimidazole-mediated-hapten-carrier-protein-conjugates
#7
Seiichi Sakamoto, Rika Nagamitsu, Gorawit Yusakul, Tomofumi Miyamoto, Hiroyuki Tanaka, Satoshi Morimoto
Monocrotaline (MCT) is a naturally occurring pyrrolizidine alkaloid mainly produced in plant genus Crotalaria (Fabaceae), in which various toxicities have been reported. Recently, demand of quantitative analysis for determination/detection of MCT has increased because MCT in plants can be transferred to human by food chain, leading to intoxication. In this study, monoclonal antibody (MAb) against MCT (MAb 4B8) was produced by unique one-pot conjugation between hydrolysate of MCT, retronecine (RN) and consecutive lysine residues of bovine-serum albumin (BSA) through N,N'-carbonyldiimidazole (CDI) mediated method...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28389280/in-vitro-safety-and-efficacy-evaluations-of-a-complex-botanical-mixture-of-eugenia-dysenterica-dc-myrtaceae-prospects-for-developing-a-new-dermocosmetic-product
#8
Larissa Cleres Moreira, Renato Ivan de Ávila, Danillo Fabrini Maciel Costa Veloso, Tatiana Nascimento Pedrosa, Emerson Silva Lima, Renê Oliveira do Couto, Eliana Martins Lima, Aline Carvalho Batista, José Realino de Paula, Marize Campos Valadares
In the context of developing a new natural product-based cosmetic, the in vitro efficacy and safety evaluations of a complex botanical mixture based on Eugenia dysenterica leaf hydroalcoholic extract (EDE) (2.5-1000μg/mL) were carried out. Chromatographic analysis demonstrated the presence of the tannin (ellagic acid) and flavonoids (quercetin and gallic acid) which characterize the EDE as a polyphenol-rich mixture. Using HFF-1 fibroblasts, it was shown that EDE promoted cell regeneration after UVA exposure...
April 4, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28383252/prescreening-of-nicotine-hapten-linkers-in-vitro-to-select-hapten-conjugate-vaccine-candidates-for-pharmacokinetic-evaluation-in-vivo
#9
Viswanath Arutla, Joseph Leal, Xiaowei Liu, Sriram Sokalingam, Michael Raleigh, Adejimi Adaralegbe, Li Liu, Paul R Pentel, Sidney M Hecht, Yung Chang
Since the demonstration of nicotine vaccines as a possible therapeutic intervention for the effects of tobacco smoke, extensive effort has been made to enhance nicotine specific immunity. Linker modifications of nicotine haptens have been a focal point for improving the immunogenicity of nicotine, in which the evaluation of these modifications usually relies on in vivo animal models, such as mice, rats or nonhuman primates. Here, we present two in vitro screening strategies to estimate and predict the immunogenic potential of our newly designed nicotine haptens...
April 17, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28383107/a-mouse-model-of-systemic-lupus-erythematosus-responds-better-to-soluble-taci-than-to-soluble-baffr-correlating-with-depletion-of-plasma-cells
#10
Philipp Haselmayer, Michele Vigolo, Josquin Nys, Pascal Schneider, Henry Hess
The TNF family cytokines B-cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL) support plasma cell survival. It is known that inhibitors of BAFF only (BAFFR-Fc) or BAFF and APRIL (TACI-Fc) administered early enough in a NZB/NZW F1 mouse model of systemic lupus erythematosus (SLE) ameliorate clinical outcomes, pointing to a pathogenic role of BAFF. In the present study, TACI-Fc administrated at a later stage of disease, after onset of autoimmunity, decreased the number of bone marrow plasma cells and slowed down further formation of autoantibodies...
April 6, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28375040/evolution-of-diagnostic-approaches-in-betalactam-hypersensitivity
#11
Tahia D Fernandez, Cristobalina Mayorga, Maria Salas, Esther Barrionuevo, Teresa Posadas, Adriana Ariza, Jose J Laguna, Esther Moreno, Maria J Torres, Inmaculada Doña, Maria I Montañez
Betalactams are the most widely used drugs against infections and the primary cause of antibiotic hypersensitivity reactions. Reaction patterns for different betalactams have been changing in accordance with consumption trends, and vary among countries. As a consequence, in vivo and in vitro tests have had to change with to keep up with new tendencies. Areas covered: This review is focused on advances in betalactam hypersensitivity diagnosis. Changes in in vivo methods have been limited to the inclusion of new haptens...
April 17, 2017: Expert Review of Clinical Pharmacology
https://www.readbyqxmd.com/read/28372729/development-of-a-thermostabilised-triplex-lamp-assay-with-dry-reagent-four-target-lateral-flow-dipstick-for-detection-of-entamoeba-histolytica-and-non-pathogenic-entamoeba-spp
#12
Phiaw Chong Foo, Yean Yean Chan, Maizan Mohamed, Weng Kin Wong, A B Nurul Najian, Boon Huat Lim
This study highlighted the development of a four target nitrocellulose-based nucleic acid lateral flow immunoassay biosensor in a dry-reagent strip format for interpretation of double-labelled double-stranded amplicons from thermostabilised triplex loop-mediated isothermal amplification assay. The DNA biosensor contained two test lines which captured biotin and texas red labelled amplicons; a LAMP internal amplification control line that captured digoxigenin labelled amplicon; and a chromatography control line that validated the functionality of the conjugated gold nanoparticles and membrane...
May 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28372197/preparation-of-monoclonal-antibody-and-development-of-an-indirect-competitive-enzyme-linked-immunosorbent-assay-for-ornidazole-detection
#13
Liang Zhao, Jiaying Li, Ye Li, Tiangang Wang, Xiaolu Jin, Kai Wang, Ebeydulla Rahman, Yuan Xing, Baoping Ji, Feng Zhou
A monoclonal antibody (mAb) and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ornidazole (ONZ) detection were developed. ONZ was conjugated with cationic bovine serum albumin as a hapten to generate the artificial immunogens and coating antigens. BALB/c mice were immunized, and mAbs were obtained. The competitive inhibition curve of ic-ELISA was y=0.0438x(2)-0.2101x+0.2925, with R(2)=0.9941. The 50% inhibition concentration, the limit of detection, and limit of quality for ONZ were 0...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28342908/intestinal-dendritic-cell-licensing-through-tlr4-is-required-for-oral-tolerance-in-allergic-contact-dermatitis
#14
Feriel Hacini-Rachinel, Mercedes Gomez de Agüero, Reem Kanjarawi, Ludovic Moro-Sibilot, Jean-Benoit Le Luduec, Claire Macari, Gilles Boschetti, Emilie Bardel, Philippe Langella, Bertrand Dubois, Dominique Kaiserlian
BACKGROUND: Induction of oral tolerance to haptens is an efficient way to prevent allergic contact dermatitis (ACD) in mice. Toll-like receptor (TLRs)-mediated sensing of the microbiota contributes to gut homeostasis, yet whether it contributes to induction of oral tolerance has not been documented. OBJECTIVE: We examined whether oral tolerance to the contact sensitizer DNFB depends on microbiota/TLRs and evaluated the role of TLR4 on the tolerogenic function of intestinal dendritic cells (DCs)...
March 22, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28326907/skin-sensitizers-in-cosmetics-and-beyond-potential-multiple-mechanisms-of-action-and-importance-of-t-cell-assays-for-in-vitro-screening
#15
Stanislav Vukmanović, Nakissa Sadrieh
Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity (DTH) reaction induced by repeated contact with sensitizers. The ability of a chemical to act as a sensitizer has most frequently been tested in animals. As the use of animals for these purposes is gradually and globally being phased out, there is a need for reliable in vitro surrogate assays. Currently proposed in vitro assays are designed to test four key events of the adverse outcome pathway (AOP) involving covalent modification of self-proteins by sensitizers (haptenation) and presentation of new antigens (hapten/carrier complexes) to the immune system...
March 22, 2017: Critical Reviews in Toxicology
https://www.readbyqxmd.com/read/28325474/corrigendum-to-epicutaneous-immunization-with-hapten-conjugated-protein-antigen-alleviates-contact-sensitivity-mediated-by-three-different-types-of-effector-cells-pharmacol-rep-64-2012-919-926
#16
Monika Majewska-Szczepanik, Anna Strzępa, Iwona Drożyńska, Sylwia Motyl, Tomasz Banach, Marian Szczepanik
No abstract text is available yet for this article.
March 16, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28320580/roles-of-alternatively-activated-m2-macrophages-in-allergic-contact-dermatitis
#17
REVIEW
Kotaro Suzuki, Kazuyuki Meguro, Daiki Nakagomi, Hiroshi Nakajima
Alternatively activated macrophages (M2 macrophages) play key roles in the suppression of Th1 cell responses and the orchestration of tissue repair. However, recent studies have shown that M2 macrophages have potentials to produce high levels of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α, suggesting that M2 macrophages may exacerbate inflammation in some settings. In this regard, we have recently shown that large numbers of M2 macrophages accumulate in the sites of hapten-induced contact hypersensitivity (CHS), an animal model of allergic contact dermatitis, and that M2 macrophages exacerbate hapten-induced CHS by producing matrix metalloproteinase 12 (MMP12)...
March 17, 2017: Allergology International: Official Journal of the Japanese Society of Allergology
https://www.readbyqxmd.com/read/28304015/sodium-periodate-mediated-conjugation-of-harringtonine-enabling-the-production-of-a-highly-specific-monoclonal-antibody-and-the-development-of-a-sensitive-quantitative-analysis-method
#18
Seiichi Sakamoto, Gorawit Yusakul, Yumi Tsuneura, Waraporn Putalun, Kazuteru Usui, Tomofumi Miyamoto, Hiroyuki Tanaka, Satoshi Morimoto
Harringtonine (HT) is a promising natural product that is mainly isolated from plants of the genus Cephalotaxus. Due to its remarkable antileukemic activities, HT has been utilized clinically in China for the treatment of acute promyelocytic leukemia (APL). No antibody that recognizes free HT has been reported to date due to the difficulty of preparing antigen conjugates in which haptens bind to a carrier protein. To overcome this difficulty, we focused on sodium periodate (NaIO4), which catalyzes unique oxidative reactions; the resulting conjugates enabled the production of a highly specific monoclonal antibody (MAb) against HT (MAb 1D2) and the establishment of an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the determination of HT...
March 17, 2017: Analyst
https://www.readbyqxmd.com/read/28295200/isothiocyanates-are-important-as-haptens-in-contact-allergy-to-chloroprene-rubber
#19
A G Ramzy, K Lammintausta, M Matura, J B Christensson, U Nilsson, L Hagvall
BACKGROUND: Contact allergy to chloroprene rubber products is well known. Thiourea compounds are considered the cause of allergy, despite being non- or weak sensitizers. Diethylthiourea is commonly occurring in this type of products and can decompose to the sensitizer ethyl isothiocyanate. OBJECTIVES: To investigate the clinical importance of degradation products and metabolites from organic thioureas in contact allergy to chloroprene rubber with focus on isothiocyanates and isocyanates...
March 10, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28294277/the-design-and-synthesis-of-an-%C3%AE-gal-trisaccharide-epitope-that-provides-a-highly-specific-anti-gal-immune-response
#20
Kensaku Anraku, Shun Sato, Nicholas T Jacob, Lisa M Eubanks, Beverly A Ellis, Kim D Janda
Carbohydrate antigens displaying Galα(1,3)Gal epitopes are recognized by naturally occurring antibodies in humans. These anti-Gal antibodies comprise up to 1% of serum IgG and have been viewed as detrimental as they are responsible for hyperacute organ rejections. In order to model this condition, α(1,3)galactosyltransferase-knockout mice are inoculated against the Galα(1,3)Gal epitope. In our study, two α-Gal trisaccharide epitopes composed of either Galα(1,3)Galβ(1,4)GlcNAc or Galα(1,3)Galβ(1,4)Glc linked to a squaric acid ester moiety were examined for their ability to elicit immune responses in KO mice...
April 5, 2017: Organic & Biomolecular Chemistry
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