Read by QxMD icon Read


Christian Adam, Jonas Wohlfarth, Maike Haußmann, Helga Sennefelder, Annette Rodin, Mareike Maler, Stefan F Martin, Matthias Goebeler, Marc Schmidt
Chromium allergy is a common occupational skin disease mediated by chromium (VI)-specific T cells that induce delayed-type hypersensitivity in sensitized individuals. Additionally, chromium (VI) can act as irritant. Both responses critically require innate immune activation, but if and how chromium (VI) elicits this signal is currently unclear.Using human monocytes, primary human keratinocytes and murine dendritic cells we show that chromium (VI) compounds fail to trigger direct proinflammatory activation but potently induce processing and secretion of IL-1β...
October 14, 2016: Journal of Investigative Dermatology
Na Sai, Wenjing Sun, Yuntang Wu, Zhong Sun, Guanggui Yu, Guowei Huang
A new enzyme-linked immunosorbent assay (ELISA) for atrazine was developed based on covalent bonding of the small molecule hapten, 2-mercaptopropionic acid-4-ethylamino-6-isopropylamino-1,3,5-triazine (MPA-atrazine), to urea-glutaraldehyde (UGA)-treated microtiter plates. In this assay, the microtiter plate surface was treated with the UGA network to both introduce amino groups, which were used to cross-link with the hapten carboxylate groups, and efficiently prevent non-specific adsorption of antibodies, which successfully eliminated the time-consuming routine blocking step...
October 12, 2016: International Immunopharmacology
Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, Richard O'Kennedy
Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilized to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It involves the exploitation of specific interactions between a binding affinity pair, such as those between an antibody and its associated antigen, or between any ligand and its associated binding receptor/protein. With the discovery of protein A in 1970, and, subsequently protein G and L, immuno-affinity chromatography has grown in popularity and is now the standard methodology for the purification of antibodies which may be implemented for a selection of different applications such as immunodiagnostics...
2017: Methods in Molecular Biology
Alicia R Mathers, Cara D Carey, Meaghan E Killeen, Julio Diaz-Perez, Sonia R Salvatore, Francisco J Schopfer, Bruce A Freeman, Louis D Falo
BACKGROUND: Reactions between nitric oxide (NO), nitrite (NO2(-) ), and unsaturated fatty acids give rise to electrophilic nitro-fatty acids (NO2 -FAs), such as nitro-oleic acid (OA-NO2 ) and nitro-linoleic acid (LNO2 ). Endogenous electrophilic fatty acids (EFAs) mediate anti-inflammatory responses by modulating metabolic and inflammatory signal transduction reactions. Hence, there is considerable interest in employing NO2 -FAs and other EFAs for the prevention and treatment of inflammatory disorders...
October 8, 2016: Allergy
Ruili Gao, Fachuang Lu, Yimin Zhu, Michael G Hahn, John Ralph
Linking lignin model compounds to carrier proteins is required in order either to raise antibodies to them or to structurally screen antibodies raised against lignins or models. We describe a flexible method to link phenolic compounds of interest to cationic bovine serum albumin (cBSA) without interfering with their important structural features. With the guaiacylglycerol-β-guaiacyl ether dimer, for example, the linking was accomplished in 89% yield with the number of dimers per carrier protein being as high as 50; NMR experiments on a 15N- and 13C-labeled conjugation product indicated that 13 dimers were added to the native lysine residues and the remainder (~37) to the amine moieties on the ethylenediamine linkers added to BSA; ~32% of the available primary amine groups on cBSA were therefore conjugated to the hapten...
October 3, 2016: Journal of Agricultural and Food Chemistry
Arun Tailor, James C Waddington, Xiaoli Meng, B Kevin Park
The covalent binding of drugs (metabolites) to proteins to form drug-protein adducts can have an adverse effect on the body. These adducts are thought to be responsible for idiosyncratic drug reactions including severe drug hypersensitivity reactions. Major advances in proteomics technology have allowed for the identification and quantification of target proteins for certain drugs. Human serum albumin (HSA) and Hb have been identified as accessible targets and potential biomarkers for drug-protein adducts formation, for numerous drugs (metabolites) including β-lactam antibiotics, reactive drug metabolites such as quinone imines (acetaminophen) and acyl glucuronides (diclofenac), and covalent inhibitors (neratinib)...
September 30, 2016: Chemical Research in Toxicology
Gloria Zhang, Christopher P Lanigan, John R Goldblum, Raymond R Tubbs, Erinn Downs-Kelly
CONTEXT: -Atypical lipomatous tumors/well-differentiated liposarcomas contain alterations in the 12q13-15 region resulting in amplification of MDM2 and nearby genes. Identifying MDM2 amplification is a useful ancillary test, as the histologic mimics of atypical lipomatous tumors/well-differentiated liposarcomas have consistently shown a lack of MDM2 amplification. OBJECTIVE: -To assess the interobserver reproducibility of a bright-field assay for MDM2 amplification (dual-color, dual-hapten in situ hybridization [DDISH]) among reviewers with varying degrees of experience with the assay and to assess the concordance of MDM2 DDISH with MDM2 fluorescence in situ hybridization (FISH)...
October 2016: Archives of Pathology & Laboratory Medicine
Luhua H Zhang, June Ho Shin, Mikel D Haggadone, John B Sunwoo
A tissue-resident population of natural killer cells (NK cells) in the liver has recently been described to have the unique capacity to confer immunological memory in the form of hapten-specific contact hypersensitivity independent of T and B cells. Factors regulating the development and maintenance of these liver-resident NK cells are poorly understood. The aryl hydrocarbon receptor (AhR) is a transcription factor modulated by exogenous and endogenous ligands that is important in the homeostasis of immune cells at barrier sites, such as the skin and gut...
September 26, 2016: Journal of Experimental Medicine
Wataru Amano, Saeko Nakajima, Yasuo Yamamoto, Atsuo Tanimoto, Mutsuyoshi Matsushita, Yoshiki Miyachi, Kenji Kabashima
BACKGROUND: Using JAK inhibitors to inhibit cytokine signaling is presumed to be a possible means of treating skin inflammatory disorders such as contact dermatitis. OBJECTIVE: To clarify the action site of JAK inhibitors in skin inflammatory disorders. METHODS: We analyzed the mechanism of action of the JAK inhibitor JTE-052 using murine skin inflammation models, including contact hypersensitivity (CHS) and irritant contact dermatitis. Cells isolated from ear tissue or lymph node (LN) were analyzed by flow cytometry...
September 13, 2016: Journal of Dermatological Science
Qi Shu, Limin Wang, Hui Ouyang, Wenwen Wang, Fengquan Liu, Zhifeng Fu
A novel bifunctional antibody (BfAb) that could recognize methyl parathion and imidacloprid simultaneously was prepared by a hybrid hybridomas technique. Using the BfAb as the sole recognition reagent, a multiplexed immunochromatographic test strip based on a time-resolved chemiluminescence (CL) strategy was developed for quantitative detection of pesticide residues. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) were used as the CL probes to label the haptens of methyl parathion and imidacloprid, respectively...
September 17, 2016: Biosensors & Bioelectronics
Takaaki Doi, Yoshiko Mizukawa, Yurie Shimoda, Yoshimi Yamazaki, Tetsuo Shiohara
Although a marked rise in the prevalence of allergic diseases over the past few decades may be related to environmental factors in industrialized countries, evidence for the protective effect of humidity on the barrier function of the skin is still awaited. We asked whether an increase in the water content of stratum corneum (SC) at the site of hapten application had a strong impact on the magnitude of contact hypersensitivity (CHS). The magnitude of CHS, induced by either lipid-soluble or water-soluble hapten, was inversely correlated with the water content of SC at the hapten application site in the elicitation phase...
September 17, 2016: Journal of Investigative Dermatology
Annika Selvick, Kari Stauss, Katrina Strobush, Lauren Taylor, Alexandra Picard, Andrea Doll, Margo Reeder
BACKGROUND: The current method for patch test tray assembly requires hand dispensing a small volume of hapten onto chambers. Because of human error, this technique produces inaccurate and inconsistent results. The recommended volume of hapten for patch testing using Finn Chambers is 20 μL. OBJECTIVE: The aims of this study were to create a device that standardizes the delivery of 20 μL and to compare it with the current hand dispensing technique. MATERIALS AND METHODS: A device, named the Revolution, was created using the SolidWorks program...
September 2016: Dermatitis
Lee Faulkner, Andrew Gibson, Andrew Sullivan, Arun Tailor, Toru Usui, Ana Alfirevic, Munir Pirmohamed, Dean J Naisbitt, B Kevin Park
A number of serious adverse drug reactions are caused by T-cells. An association with HLA alleles has been identified with certain reactions, which makes it difficult to develop standardized preclinical tests to predict chemical liability. We have recently developed a T cell priming assay using the drug metabolite nitroso sulfamethoxazole (SMX-NO). We now report on reproducibility of the assay, establishment of a biobank of PBMC from 1000 HLA-typed volunteers, and generation of antigen-specific responses to a panel of compounds...
September 16, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
Pramodkumar D Jadhav, Youn Young Shim, Martin J T Reaney
Bioactive flax cyclic peptides (orbitides, linusorbs) were site specifically ligated through methionine with bovine serum albumin (BSA) to produce immunogenic compounds. In this study, modified flaxseed immunosuppressant orbitides (linusorbs, LOs) containing hydroxyl (OH) groups were synthesized for use as haptens. These compounds were extensively characterized by 1H-NMR, 13C-NMR, HPLC-MS/MS, and FTIR. The haptens were conjugated to BSA and the extent of hapten incorporation was determined by MALDI, LC-ESI-MS, and SDS-PAGE...
September 14, 2016: Bioconjugate Chemistry
Karsten Mahnke, Jurgina Useliene, Sabine Ring, Paula Kage, Verena Jendrossek, Simon C Robson, Matilda Bylaite-Bucinskiene, Kerstin Steinbrink, Alexander H Enk
Injection of regulatory T cells (Treg) followed by sensitization with TNCB induced a transient increase in size and cellularity of skin draining lymph nodes (LN) in mice. This led us to hypothesize that Treg may affect the trafficking of T cells from - and to peripheral LN. Two to three hours after sensitization we found fewer CD8(+) T cells expressing CD62L in LN as compared to untreated controls. Injection of wildtype Treg prevented this down-regulation of CD62L. In contrast, Treg devoid of the ATP-degrading ecto-enzyme CD39 were unable to do so...
September 10, 2016: Journal of Investigative Dermatology
Zhenlai Zhu, Yang Luo, Jinlei Yu, Jixin Gao, Yueqiang Zhang, Chunying Xiao, Chen Zhang, Gang Wang, Yufeng Liu, Meng Fu, Xu Yao, Wei Li
Originally recognized as a regulator of axon guidance in the nervous system, Semaphorin 4D (Sema4D, CD100) also participates in various immune responses and many immune-related diseases. However, whether Sema4D is involved in the pathogenesis of contact hypersensitivity (CHS) remains unclear. In this study, we explored the role of Sema4D in oxazolone-induced CHS using Sema4D knockout (KO) mice. We found that Sema4D KO mice developed attenuated CHS responses, as indicated by milder ear-swelling, lower expression of IL-1β, IL-6, CXCL2 and CXCL5, and decreased recruitment of neutrophils, CD8(+) T cells and CD4(+) T cells...
October 2016: Molecular Immunology
Xiaoli Meng, Caroline J Earnshaw, Arun Tailor, Rosalind E Jenkins, James C Waddington, Paul Whitaker, Neil S French, Dean J Naisbitt, B Kevin Park
Amoxicillin-clavulanate (AC) is one of the most common causes of drug induced liver injury (DILI). The association between AC-DILI and HLA alleles and the detection of drug-specific T cells in patients with AC-DILI indicate that the adaptive immune system is involved in the disease pathogenesis. In this study, mass spectrometric methods were employed to characterize the antigen formed by AC in exposed patients and the antigenic determinants that stimulate T cells. Amoxicillin formed penicilloyl adducts with lysine residues on human serum albumin (HSA) in vitro, with K190 and K199 being the most reactive sites...
October 17, 2016: Chemical Research in Toxicology
Mats Bemark, Helena Hazanov, Anneli Strömberg, Rathan Komban, Joel Holmqvist, Sofia Köster, Johan Mattsson, Per Sikora, Ramit Mehr, Nils Y Lycke
Understanding how memory B cells are induced and relate to long-lived plasma cells is important for vaccine development. Immunity to oral vaccines has been considered short-lived because of a poor ability to develop IgA B-cell memory. Here we demonstrate that long-lived mucosal IgA memory is readily achieved by oral but not systemic immunization in mouse models with NP hapten conjugated with cholera toxin and transfer of B1-8(high)/GFP(+) NP-specific B cells. Unexpectedly, memory B cells are poorly related to long-lived plasma cells and less affinity-matured...
2016: Nature Communications
Yali Zhou, Pingshi Wang, Jie Xiong, Huan Yue, Yong He, Hui Ouyang, Lin Wang, Zhifeng Fu
It is of great importance to measure antibody affinity in the course of screening monoclonal antibody (McAb) for immunotherapy, immunoassay and immunological purification. Herein, by using terbutaline mouse McAb as a model, a novel label-free strategy based on on-line microdialysis (MD) sampling combined with flow injection chemiluminescent detection was designed for measuring antibody affinity to hapten in a homogeneous system. After this McAb incubated with its hapten, the unbound hapten was sampled on-line by the MD probe and injected into the chemiluminescent detection system for quantification...
August 20, 2016: Biosensors & Bioelectronics
Paola Lucia Minciullo, Giovanni Paolino, Maddalena Vacca, Sebastiano Gangemi, Eustachio Nettis
The oral mucosa including the lips is constantly exposed to several noxious stimuli, irritants and allergens. However, oral contact pathologies are not frequently seen because of the relative resistance of the oral mucosa to irritant agents and allergens due to anatomical and physiological factors. The spectrum of signs and symptoms of oral contact allergies (OCA) is broad and a large number of condition can be the clinical expression of OCA such as allergic contact stomatitis, allergic contact cheilitis, geographic tongue, oral lichenoid reactions, burning mouth syndrome...
2016: Clinical and Molecular Allergy: CMA
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"