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https://www.readbyqxmd.com/read/28629039/time-dependent-subcellular-structure-injuries-induced-by-nano-micron-sized-calcium-oxalate-monohydrate-and-dihydrate-crystals
#1
Xin-Yuan Sun, Kai Yu, Jian-Ming Ouyang
Comparative studies were conducted to investigate the time effect of cell injury induced by nano-sized (50nm) and micron-sized (10μm) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals in African green monkey renal epithelial (Vero) cells. The effects of nano-/micron-sized COM and COD exposure on Vero cells were investigated by detecting the cell viability, cell morphology, LDH release, reactive oxygen species, mitochondrial membrane potential, cell cycle, and cell apoptosis, as well as the intracellular and extracellular crystal distribution...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627733/response-of-renal-tubular-cells-to-differential-types-and-doses-of-calcium-oxalate-crystals-integrative-proteome-network-analysis-and-functional-investigations
#2
Arada Vinaiphat, Siripat Aluksanasuwan, Juthatip Manissorn, Suchitra Sutthimethakorn, Visith Thongboonkerd
We have previously identified changes in the cellular proteome of renal tubular cells induced by low-dose (100 μg/ml) and high-dose (1,000 μg/ml) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals. However, the functional significance of such expression data remained unclear. In this study, we performed comparative analyses and functional investigations of four proteomic datasets to define potential mechanisms by which renal tubular cells responded to differential crystal types and doses. The data showed that high-dose induced greater changes than low-dose, whereas COM induced more changes than COD...
June 19, 2017: Proteomics
https://www.readbyqxmd.com/read/28623397/different-effects-of-%C3%AE-linolenic-acid-gla-supplementation-on-plasma-and-red-blood-cell-phospholipid-fatty-acid-composition-and-calcium-oxalate-kidney-stone-risk-factors-in-healthy-subjects-from-two-race-groups-with-different-risk-profiles-pose-questions-about
#3
Allen L Rodgers, Dalielah Jappie-Mahomed, Paul J van Jaarsveld
Fatty acid (FA) composition of phospholipids in plasma and red blood cells (RBC) can influence calciuria, oxaluria and renal stone formation. In this regard, the ratio of arachidonic acid (AA) and its precursor linoleic acid (LA) appears to be important. Administration of γ-linolenic acid (GLA) has been shown to increase the concentration of dihomo-gamma linoleic acid (DGLA) relative to AA indicating that it may attenuate biosynthesis of the latter. Such effects have not been investigated in race groups having difference stone occurrence rates...
June 16, 2017: Urolithiasis
https://www.readbyqxmd.com/read/28621639/outcome-of-the-double-j-stent-placement-in-pediatric-kidney-transplant-a-single-center-experience
#4
Hakan Sözen, Onur Özen, Kibriya Fidan, Oğuz Söylemezoğlu, Aydın Dalgıç
OBJECTIVES: Double J stent placement at kidney transplant may reduce stenosis or leakage complication rates. However, placement may also increase risk for early urinary tract infection (ie, < 3 mo after transplant). In children, the usefulness of double J stent placement is not well defined. MATERIALS AND METHODS: We analyzed retrospective data from children who received transplants at the Gazi University Transplantation Center and Pediatric Nephrology (Ankara, Turkey) for outcomes related to double J stents...
June 16, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28616648/protective-effect-of-dietary-polyphenol-caffeic-acid-on-ethylene-glycol-induced-kidney-stones-in-rats
#5
Fauzia Yasir, Atia-Tul- Wahab, M Iqbal Choudhary
Dietary polyphenol caffeic acid (1) has been reported for various pharmacological activities. The aim of the current study was to investigate the effect of caffeic acid (1) on ethylene glycol-induced renal stones in rats. For the study, male Wistar rats were divided into seven groups; normal, pathological, and standard drug controls, and preventive and curative groups. Normal control group received drinking water for 8 weeks. Pathological, standard drug, preventive, and curative groups received 0.75% ethylene glycol in drinking water for the induction of calcium oxalate stone formation, along with the regular diet...
June 14, 2017: Urolithiasis
https://www.readbyqxmd.com/read/28613206/-evolution-from-percutaneous-nephrolithotomy-to-mini-pcnl-in-supine-position-on-the-treatment-of-complex-renal-calculi-feasibility-study
#6
Fernando Ramón de Fata-Chillón, Helena Gimbernat-Díaz, Cristina Redondo-Redondo, Elisa Meilán-Hernández, Erika Mateo-Martínez
OBJECTIVES: Mini-PCNL is a potentially less invasive technique than standard percutaneous nephrolithotomy (PCNL). We present our experience and results comparing both approaches in large burden complex renal calculi. METHODS: Prospective non randomized study comparing PCNL (24/26F nephroscope; Group A) and Mini-PCNL (15/18F; Group B) perioperative and postoperative results, in 40 (20 each group) consecutive patients between 2013 and 2014. We analyze demographic data, hemoglobin drop, urine culture, stone characteristics, operative time, puncture, number and size of the tract, disintegration energy sources, nephrostomy placement, hospital stay, stone free rate and Clavien-Dindo complications...
June 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28613205/-renal-lithiasis-can-we-predict-the-need-for-retreatment-in-retrograde-intrarenal-surgery-rirs
#7
José Luis Palmero-Martí, Jorge Panach-Navarrete, Lorena Valls-González, Amparo Ganau-Ituren, Juan Carlos Pastor-Lence, Antonio Benedicto-Redón
OBJECTIVES: To analyze the predictive factors for retreatment in RIRS to achieve complete lithiasis resolution. METHODS: Retrospective comparative study analyzing 298 cases of RIRS performed in our center over a 3 year period. The cohort was divided in two groups: Resolution in one operation or more than one, evaluating homogeneicity for age and gender. We compared the folowing variables: Hounsfield units, body mass index (BMI), number of stones, size, (on the case of multiple stones, larger stone size), side, location in the kidney and stone biochemistry...
June 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28605509/a-novel-homozygous-umod-mutation-reveals-gene-dosage-effects-on-uromodulin-processing-and-urinary-excretion
#8
Noel Edwards, Eric Olinger, Jennifer Adam, Michael Kelly, Guglielmo Schiano, Simon A Ramsbottom, Richard Sandford, Olivier Devuyst, John A Sayer
Heterozygous mutations in UMOD encoding the urinary protein uromodulin are the most common genetic cause of autosomal dominant tubulointerstitial kidney disease (ADTKD). We describe the exceptional case of a patient from a consanguineous family carrying a novel homozygous UMOD mutation (p.C120Y) affecting a conserved cysteine residue within the EGF-like domain III of uromodulin. Comparison of heterozygote and homozygote mutation carriers revealed a gene dosage effect with unprecedented low levels of uromodulin and aberrant uromodulin fragments in the urine of the homozygote proband...
June 10, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28592876/elongation-factor-tu-on-escherichia-coli-isolated-from-urine-of-kidney-stone-patients-promotes-calcium-oxalate-crystal-growth-and-aggregation
#9
Piyawan Amimanan, Ratree Tavichakorntrakool, Kedsarin Fong-Ngern, Pipat Sribenjalux, Aroonlug Lulitanond, Vitoon Prasongwatana, Chaisiri Wongkham, Patcharee Boonsiri, Jariya Umka Welbat, Visith Thongboonkerd
Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100 stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28589217/a-prospective-randomized-comparison-of-micropercutaneous-nephrolithotomy-microperc-and-retrograde-intrarenal-surgery-rirs-for-the-management-of-lower-pole-kidney-stones
#10
Abdulkadir Kandemir, Selcuk Guven, Mehmet Balasar, Mehmet Giray Sonmez, Hakan Taskapu, Recai Gurbuz
OBJECTIVE: To make a comparison between the safety and efficacy of micropercutaneous nephrolithotomy (microperc) and retrograde intrarenal surgery (RIRS) for the management of lower pole kidney stones up to 15 mm. PATIENTS AND METHODS: 60 patients presenting with solitary lower pole kidney stones up to 15 mm were included in the study between March 2013 and December 2015. Patients were randomized into Microperc or RIRS groups with computer-generated numbers. RESULTS: The mean stone size was 10...
June 6, 2017: World Journal of Urology
https://www.readbyqxmd.com/read/28585182/lime-powder-treatment-reduces-urinary-excretion-of-total-protein-and-transferrin-but-increases-uromodulin-excretion-in-patients-with-urolithiasis
#11
Piyaratana Tosukhowong, Pimsuda Kulpradit, Sakdithep Chaiyarit, Wattanachai Ungjareonwattana, Nuttiya Kalpongnukul, Supoj Ratchanon, Visith Thongboonkerd
Our previous study has shown that lime powder (LP) had an inhibitory effect against calcium oxalate stone formation. However, the precise mechanisms underlying such beneficial effect remained unclear. Our present study thus aimed to address the effect of LP on excretory level and compositions of urinary proteins using a proteomics approach. From a total of 80 calcium oxalate stone formers recruited into our 2-year randomized clinical trial of LP effect, 10 patients with comparable age and clinical parameters were selected for this proteomic study...
June 5, 2017: Urolithiasis
https://www.readbyqxmd.com/read/28585181/which-way-is-best-for-stone-fragments-and-dust-extraction-during-percutaneous-nephrolithotomy
#12
Bulent Kati, Eyyup Sabri Pelit, Ismail Yagmur, Yigit Akin, Halil Ciftci, Ercan Yeni
Percutaneous nephrolithotomy (PCNL) is a commonly used type of minimally invasive treatment in kidney stone surgeries. Surgical success is assessed according to residual stone amount after surgery. The purpose of this study is to compare the two methods' success and practicality that are applied after the fracture of the stone in the patients who applied PCNL and which enable the removal of the residual stones. Among 102 patients who underwent a single-session of PCNL at our department between June 2015 and November 2016 were evaluated...
June 5, 2017: Urolithiasis
https://www.readbyqxmd.com/read/28584897/effects-of-juice-processing-on-oxalate-contents%C3%A2-in-carambola-juice-products
#13
Nha K Huynh, Ha V H Nguyen
Effects of processing methods including pressing, enzyme-assisted extraction, lactic acid fermentation by Lactobacillus acidophilus, and alcohol fermentation by Saccharomyces cerevisiae on total and soluble oxalate contents of carambola juices were studied. In comparison with pressing, the use of enzyme increased juice yields (15.89-17.29%), but resulted in higher total oxalate (1.60-1.73 times) and soluble oxalate contents (1.16-1.49 times). In addition, extension of enzyme incubation periods led to an increase in soluble oxalate contents in the products (p < 0...
June 6, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28567512/total-flavonoids-of-desmodium-styracifolium-attenuates-the-formation-of-hydroxy-l-proline-induced-calcium-oxalate-urolithiasis-in-rats
#14
Jianfu Zhou, Jing Jin, Xiong Li, Zhongxiang Zhao, Lei Zhang, Qian Wang, Jing Li, Qiuhong Zhang, Songtao Xiang
Desmosium styracifolium (D. styracifolium), which is considered as a Chinese herbal medicine, has been reported to treat the kidney stone diseases. However, the potential phytochemically active components and the underlying mechanisms associated with its efficacy in targeting urolithiasis remain to be elucidated. This study aims to investigate the anti-urolithiatic effect of total flavonoids of D. styracifolium (TFDS) on calcium oxalate (CaOx) renal stones in Sprague-Dawley rats. Animal models of CaOx urolithiasis were established in male Sprague-Dawley rats by adding 5% w/w hydroxy-L-proline (HLP) in regular rat chow...
May 31, 2017: Urolithiasis
https://www.readbyqxmd.com/read/28552742/oxalobacter-formigenes-colonization-normalizes-oxalate-excretion-in-a-gastric-bypass-model-of-hyperoxaluria
#15
Benjamin K Canales, Marguerite Hatch
BACKGROUND: Hyperoxaluria and oxalate kidney stones frequently develop after Roux-en-Y gastric bypass (RYGB). Oxalobacter formigenes can degrade ingested oxalate. OBJECTIVES: Examine the effect of O. formigenes wild rat strain (OXWR) colonization on urinary oxalate excretion and intestinal oxalate transport in a hyperoxaluric RYGB model. SETTING: Basic Science Laboratory, United States. METHODS: At 21 weeks of age, 28 obese male Sprague-Dawley rats survived Sham (n = 10) or RYGB (n = 18) surgery and were maintained on a 1...
March 23, 2017: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/28537452/safety-and-effectiveness-of-a-longer-focal-beam-and-burst-duration-in-ultrasonic-propulsion-for-repositioning-urinary-stones-and-fragments
#16
Karmon M Janssen, Timothy C Brand, Bryan W Cunitz, Yak-Nam Wang, Julianna C Simon, Frank Starr, H Denny Liggitt, Jeff Thiel, Mathew D Sorensen, Jonathan D Harper, Michael R Bailey, Barbrina Dunmire
PURPOSE: In the first-in-human trial of ultrasonic propulsion, subjects passed collections of residual stone fragments repositioned with a C5-2 probe. Here, effectiveness and safety in moving multiple fragments are compared between the C5-2 and a custom (SC-50) probe that produces a longer focal beam and burst duration. MATERIALS AND METHODS: Effectiveness was quantified by the number of stones expelled from a calyx phantom consisting of a 30-mm deep, water-filled well in a block of tissue mimicking material...
June 26, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28537418/short-term-changes-in-algometry-inclinometry-stabilometry-and-urinary-ph-analysis-after-a-thoracolumbar-junction-manipulation-in-patients-with-kidney-stones
#17
Ángel Oliva Pascual-Vaca, Ramón Punzano-Rodríguez, Pablo Escribá-Astaburuaga, Juan Carlos Fernández-Domínguez, François Ricard, Maria Angeles Franco-Sierra, Cleofás Rodríguez-Blanco
OBJECTIVES: To determine the efficacy of a high-velocity low-amplitude manipulation of the thoracolumbar junction in different urologic and musculoskeletal parameters in subjects suffering from renal lithiasis. DESIGN: Randomized, controlled blinded clinical study. SETTINGS/LOCATION: The Nephrology departments of two hospitals and one private consultancy of physiotherapy in Valencia (Spain). SUBJECTS: Forty-six patients suffering from renal lithiasis...
May 24, 2017: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/28537042/safety-and-efficacy-of-percutaneous-nephrolithotomy-in-patients-with-severe-skeletal-deformities
#18
Seyed Habibollah Mousavi-Bahar, Shahriar Amirhasani, Maede Mohseni, Rezgar Daneshdoost
PURPOSE: Treatment of renal calculi in patients with severe skeletal deformities can be challenging. We present our experience in order to provide an assessment of technical difficulties, associated complications, and outcomes of percutaneous nephrolithotomy (PCNL) as a treatment option in this special patient group. MATERIALS AND METHODS: Our study included eight patients treated with PCNL for renal stones. All had severe skeletal deformities including six with severe kyphoscoliosis, one with osteogenesis imperfecta, and another withrickets...
May 23, 2017: Urology Journal
https://www.readbyqxmd.com/read/28528955/potential-therapeutic-activity-of-phlogacanthus-thyrsiformis-hardow-mabb-flower-extract-and-its-biofabricated-silver-nanoparticles-against-chemically-induced-urolithiasis-in-male-wistar-rats
#19
Poppy Das, Kiran Kumar, Arunai Nambiraj, Reshma Rajan, Rajendra Awasthi, Kamal Dua, Himaja M
Urolithiasis is a painful disorder in which stones are formed in the kidney, bladder or urethra. There are no proper therapeutic treatments available for kidney stones and people suffering from larger stones have to undergo surgery which has many side effects. A natural remedy with therapeutic effects that can dissipate and remove even the larger stones would eliminate the need of a surgery and the risks associated with it. The flowers of Phlogacanthus thyrsiformis used in culinary recipes in the north eastern India are also widely used as a folklore medicine for the treatment of kidney stones and liver disorders...
May 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28521550/detection-and-evaluation-of-renal-injury-in-burst-wave-lithotripsy-using-ultrasound-and-magnetic-resonance-imaging
#20
Philip Clark May, Wayne Kreider, Adam D Maxwell, Yak-Nam Wang, Bryan W Cunitz, Philip M Blomgren, Cynthia Johnson, Joshua Sh Park, Michael R Bailey, Donghoon Lee, Jonathan D Harper, Mathew D Sorensen
PURPOSE: Burst wave lithotripsy (BWL) is a transcutaneous technique with potential to safely and effectively fragment renal stones. Preclinical investigations of BWL require the assessment of potential renal injury. This study evaluates the capabilities of real-time ultrasound and magnetic resonance imaging (MRI) to detect and evaluate BWL injury that was induced in porcine kidneys. MATERIALS AND METHODS: Ten kidneys from five female farm pigs were treated with either a 170 kHz or 335 kHz BWL transducer using variable treatment parameters and monitored in real time with ultrasound...
May 18, 2017: Journal of Endourology
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