keyword
https://read.qxmd.com/read/38180232/3d-hydrogel-encapsulation-regulates-nephrogenesis-in-kidney-organoids
#21
JOURNAL ARTICLE
Bryan A Nerger, Sumit Sinha, Nathan N Lee, Maria Cheriyan, Pascal Bertsch, Christopher P Johnson, L Mahadevan, Joseph V Bonventre, David J Mooney
Stem cell-derived kidney organoids contain nephron segments that recapitulate morphological and functional aspects of the human kidney. However, directed differentiation protocols for kidney organoids are largely conducted using biochemical signals to control differentiation. Here, the hypothesis that mechanical signals regulate nephrogenesis is investigated in 3D culture by encapsulating kidney organoids within viscoelastic alginate hydrogels with varying rates of stress relaxation. Tubular nephron segments are significantly more convoluted in kidney organoids differentiated in encapsulating hydrogels when compared with those in suspension culture...
January 5, 2024: Advanced Materials
https://read.qxmd.com/read/38149596/a-novel-hydrodynamic-design-greatly-improves-the-debris-clearance-rate-of-flexible-ureteroscopy
#22
JOURNAL ARTICLE
WeiSong Wu, Junyi Yang, Wenlong Wan, RiXiaTi Yi, Xian Li, Jiaqiao Zhang, XueCheng Hu, Wei Liu, Ping Wang, Jinping Li, Shaogang Wang, Xiao Yu
OBJECTIVES: To introduce a novel hydrodynamic design for a flexible ureteroscope that can increase stone debris clearance. METHODS: Based on hydrodynamics, the new design allowed the ureteroscope to have six water jets. Fluid gushed from the six jets and would ultimately converge into an eddy. The safety and stone debris clearance efficiency were tested in a 3D-printed kidney model. Stone fragments between 0.5 and 1 mm were used to mimic the debris. A ureteroscope already approved for marketing was used as a control...
December 27, 2023: Journal of Endourology
https://read.qxmd.com/read/38135963/blobcut-a-contrastive-learning-method-to-support-small-blob-detection-in-medical-imaging
#23
JOURNAL ARTICLE
Teng Li, Yanzhe Xu, Teresa Wu, Jennifer R Charlton, Kevin M Bennett, Firas Al-Hindawi
Medical imaging-based biomarkers derived from small objects (e.g., cell nuclei) play a crucial role in medical applications. However, detecting and segmenting small objects (a.k.a. blobs) remains a challenging task. In this research, we propose a novel 3D small blob detector called BlobCUT. BlobCUT is an unpaired image-to-image (I2I) translation model that falls under the Contrastive Unpaired Translation paradigm. It employs a blob synthesis module to generate synthetic 3D blobs with corresponding masks. This is incorporated into the iterative model training as the ground truth...
November 29, 2023: Bioengineering
https://read.qxmd.com/read/38129901/precision-nephrotoxicity-testing-using-3d-in-vitro-models
#24
REVIEW
Pengfei Yu, Hainan Zhu, Carol Christine Bosholm, Daniella Beiner, Zhongping Duan, Avinash K Shetty, Steve S Mou, Philip Adam Kramer, Luis F Barroso, Hongbing Liu, Kun Cheng, Michael Ihnat, Matthew A Gorris, Joseph A Aloi, Jobira A Woldemichael, Anthony Bleyer, Yuanyuan Zhang
Nephrotoxicity is a significant concern during the development of new drugs or when assessing the safety of chemicals in consumer products. Traditional methods for testing nephrotoxicity involve animal models or 2D in vitro cell cultures, the latter of which lack the complexity and functionality of the human kidney. 3D in vitro models are created by culturing human primary kidney cells derived from urine in a 3D microenvironment that mimics the fluid shear stresses of the kidney. Thus, 3D in vitro models provide more accurate and reliable predictions of human nephrotoxicity compared to existing 2D models...
December 21, 2023: Cell & Bioscience
https://read.qxmd.com/read/38059598/zoledronic-acid-and-ibandronate-induced-nephrotoxicity-in-2d-and-3d-proximal-tubule-cells-derived-from-human-and-rat
#25
JOURNAL ARTICLE
Leslie J Valencia, Min Tseng, Mei-Lan Chu, Lanlan Yu, Adeyemi O Adedeji, Tomomi Kiyota
Drug-induced proximal tubule (PT) injury remains a serious safety concern throughout drug development. Traditional in vitro 2D and preclinical in vivo models often fail to predict drug-related injuries presented in clinical trials. Various 3D microphysiological systems (MPSs) have been developed to mimic physiologically relevant properties, enabling them to be more predictive towards nephrotoxicity. To explore the capabilities of an MPS across species, we compared cytotoxicity in hRPTEC/TERT1s and rat primary proximal epithelial tubule cells (rPPTECs) following exposure to zoledronic acid and ibandronate (62...
December 6, 2023: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://read.qxmd.com/read/38048731/a-fully-automated-machine-learning-based-methodology-for-personalized-radiation-dose-assessment-in-thoracic-and-abdomen-ct
#26
JOURNAL ARTICLE
Eleftherios Tzanis, John Stratakis, Marios Myronakis, John Damilakis
PURPOSE: To develop a machine learning-based methodology for patient-specific radiation dosimetry in thoracic and abdomen CT. METHODS: Three hundred and thirty-one thoracoabdominal radiotherapy-planning CT examinations with the respective organ/patient contours were collected retrospectively for the development and validation of segmentation 3D-UNets. Moreover, 97 diagnostic thoracic and 89 diagnostic abdomen CT examinations were collected retrospectively. For each of the diagnostic CT examinations, personalized MC dosimetry was performed...
December 3, 2023: Physica Medica: PM
https://read.qxmd.com/read/38041911/a-comprehensive-review-on-3d-tissue-models-biofabrication-technologies-and-preclinical-applications
#27
REVIEW
Renjian Xie, Vaibhav Pal, Yanrong Yu, Xiaolu Lu, Mengwei Gao, Shijie Liang, Miao Huang, Weijie Peng, Ibrahim T Ozbolat
The limitations of traditional two-dimensional (2D) cultures and animal testing, when it comes to precisely foreseeing the toxicity and clinical effectiveness of potential drug candidates, have resulted in a notable increase in the rate of failure during the process of drug discovery and development. Three-dimensional (3D) in-vitro models have arisen as substitute platforms with the capacity to accurately depict in-vivo conditions and increasing the predictivity of clinical effects and toxicity of drug candidates...
November 27, 2023: Biomaterials
https://read.qxmd.com/read/38020712/decellularized-diseased-tissues-current-state-of-the-art-and-future-directions
#28
REVIEW
Xiang Li, Jianyang Shan, Xin Chen, Haomin Cui, Gen Wen, Yaling Yu
Decellularized matrices derived from diseased tissues/organs have evolved in the most recent years, providing novel research perspectives for understanding disease occurrence and progression and providing accurate pseudo models for developing new disease treatments. Although decellularized matrix maintaining the native composition, ultrastructure, and biomechanical characteristics of extracellular matrix (ECM), alongside intact and perfusable vascular compartments, facilitates the construction of bioengineered organ explants in vitro and promotes angiogenesis and tissue/organ regeneration in vivo, the availability of healthy tissues and organs for the preparation of decellularized ECM materials is limited...
December 2023: MedComm
https://read.qxmd.com/read/38007296/a-novel-model-based-equation-for-size-dependent-mean-recovery-coefficients-for-spheres-and-other-shapes
#29
JOURNAL ARTICLE
Robin de Nijs
BACKGROUND: In NM-imaging, theoretical curves for the recovery coefficient (RC) of the signal maximum and mean are known for spheres and cubes, if a 3D Gaussian PSF is assumed. The RC of the maximum is also known for cylinders. For these and other shapes empirical equations with one or two fit-parameters have been utilized. METHODS: An equation for the RC for large objects of arbitrary shape is derived and generalized into an empirical equation for smaller objects, which is verified by numerical simulations...
November 24, 2023: Physica Medica: PM
https://read.qxmd.com/read/37998590/3d-model-artificial-intelligence-guided-automatic-augmented-reality-images-during-robotic-partial-nephrectomy
#30
Michele Sica, Pietro Piazzolla, Daniele Amparore, Paolo Verri, Sabrina De Cillis, Federico Piramide, Gabriele Volpi, Alberto Piana, Michele Di Dio, Stefano Alba, Cecilia Gatti, Mariano Burgio, Giovanni Busacca, Angelo Giordano, Cristian Fiori, Francesco Porpiglia, Enrico Checcucci
More than ever, precision surgery is making its way into modern surgery for functional organ preservation. This is possible mainly due to the increasing number of technologies available, including 3D models, virtual reality, augmented reality, and artificial intelligence. Intraoperative surgical navigation represents an interesting application of these technologies, allowing to understand in detail the surgical anatomy, planning a patient-tailored approach. Automatic superimposition comes into this context to optimally perform surgery as accurately as possible...
November 16, 2023: Diagnostics
https://read.qxmd.com/read/37997523/3d-printed-tubulointerstitium-chip-as-an-in-vitro-testing-platform
#31
JOURNAL ARTICLE
Gabriele Addario, Daphne Eussen, Sonja Djudjaj, Peter Boor, Lorenzo Moroni, Carlos Mota
Chronic kidney disease (CKD) ranks as the twelfth leading cause of death worldwide and represents a major global health problem with still rather limited treatment options. The development of new in vitro models replicating defined segments of the kidney functional units, i.e., the nephrons, in a physiologically relevant and reproducible manner could facilitate drug testing and development. We aimed at producing an in vitro organ-on-a-chip platform with extrusion-based 3D printing approach with sacrificial components...
November 23, 2023: Macromolecular Bioscience
https://read.qxmd.com/read/37958709/efficacy-of-mesenchymal-stromal-cell-derived-extracellular-vesicles-in-ameliorating-cisplatin-nephrotoxicity-as-modeled-using-three-dimensional-gravity-driven-two-layer-tubule-on-a-chip-3d-motive-chip
#32
JOURNAL ARTICLE
Eun-Jeong Kwon, Seong-Hye Hwang, Seungwan Seo, Jaesung Park, Seokwoo Park, Sejoong Kim
Mesenchymal stromal cell (MSC)-derived extracellular vesicles (EVs) are known to have a therapeutic effect on nephrotoxicity. As animal models require significant time and resources to evaluate drug effects, there is a need for a new experimental technique that can accurately predict drug effects in humans. We evaluated the therapeutic effect of MSC-derived EVs in cisplatin nephrotoxicity using a three-dimensional, gravity-driven, two-layer tubule-on-a-chip (3D-MOTIVE chip). In the 3D-MOTIVE chip, 10 μM cisplatin decreased the number of attached cells compared to the vehicle...
October 29, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37958530/delivery-and-transcriptome-assessment-of-an-in-vitro-three-dimensional-proximal-tubule-model-established-by-human-kidney-2-cells-in-clinical-gelatin-sponges
#33
JOURNAL ARTICLE
Hui-Yi Hsiao, Tzung-Hai Yen, Fang-Yu Wu, Chao-Min Cheng, Jia-Wei Liu, Yu-Ting Fan, Jung-Ju Huang, Chung-Yi Nien
The high prevalence of kidney diseases and the low identification rate of drug nephrotoxicity in preclinical studies reinforce the need for representative yet feasible renal models. Although in vitro cell-based models utilizing renal proximal tubules are widely used for kidney research, many proximal tubule cell (PTC) lines have been indicated to be less sensitive to nephrotoxins, mainly due to altered expression of transporters under a two-dimensional culture (2D) environment. Here, we selected HK-2 cells to establish a simplified three-dimensional (3D) model using gelatin sponges as scaffolds...
October 24, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37952029/reconfiguration-and-loss-of-peritubular-capillaries-in-chronic-kidney-disease
#34
JOURNAL ARTICLE
Charlotte Gaupp, Benjamin Schmid, Philipp Tripal, Aurélie Edwards, Christoph Daniel, Stefan Zimmermann, Margarete Goppelt-Struebe, Carsten Willam, Seymour Rosen, Gunnar Schley
Functional and structural alterations of peritubular capillaries (PTCs) are a major determinant of chronic kidney disease (CKD). Using a software-based algorithm for semiautomatic segmentation and morphometric quantification, this study analyzes alterations of PTC shape associated with chronic tubulointerstitial injury in three mouse models and in human biopsies. In normal kidney tissue PTC shape was predominantly elongated, whereas the majority of PTCs associated with chronic tubulointerstitial injury had a rounder shape...
November 11, 2023: Scientific Reports
https://read.qxmd.com/read/37942584/ascorbate-protects-human-kidney-organoids-from-damage-induced-by-cell-free-hemoglobin
#35
JOURNAL ARTICLE
Julie Bejoy, Justin M Farry, Eddie S Qian, Curtis H Dearing, Lorraine B Ware, Julie A Bastarache, Lauren E Woodard
Sepsis-associated acute kidney injury is associated with high morbidity and mortality in critically ill patients. Cell-free hemoglobin (CFH) is released into the circulation of patients with severe sepsis and the levels of CFH are independently associated with mortality. CFH treatment increased cytotoxicity in the human tubular epithelial cell line HK-2. To better model the intact kidney, we cultured human kidney organoids derived from induced pluripotent stem cells. We treated human kidney organoids grown by both three-dimensional (3D) and transwell protocols with CFH for 48 hours...
November 9, 2023: Disease Models & Mechanisms
https://read.qxmd.com/read/37915907/development-of-a-multimodal-kidney-age-prediction-based-on-automatic-segmentation-ct-image-in-patients-with-normal-renal-function
#36
JOURNAL ARTICLE
Zuoxian Hou, Gumuyang Zhang, Yixin Ma, Peng Xia, Xiaoxiao Shi, Wenlong She, Tianzuo Zhao, Hao Sun, Zhengguang Chen, Limeng Chen
BACKGROUND: For decades, description of renal function has been of interest to clinicians and researchers. Serum creatinine (Scr) and estimated glomerular filtration rate (eGFR) are familiar but also limited in many circumstances. Meanwhile, the physiological volumes of the kidney cortex and medulla are presumed to change with age and have been proven to change with decreasing kidney function. METHODS: We recruited 182 patients with normal Scr levels between October 2021 and February 2022 in Peking Union Medical College Hospital (PUMCH) with demographic and clinical data...
November 2023: Clinical Kidney Journal
https://read.qxmd.com/read/37910400/nanodiscoidal-nucleic-acids-for-gene-regulation
#37
JOURNAL ARTICLE
Radhika Sharma, Steven Narum, Shuhong Liu, Yixiao Dong, Kyung In Baek, Hanjoong Jo, Khalid Salaita
Therapeutic nucleic acids represent a powerful class of drug molecules to control gene expression and protein synthesis. A major challenge in this field is that soluble oligonucleotides have limited serum stability, and the majority of nucleic acids that enter the cells are trapped within endosomes. Delivery efficiency can be improved using lipid scaffolds. One such example is the nanodisc (ND), a self-assembled nanostructure composed of phospholipids and peptides and modeled after high density lipoproteins (HDLs)...
November 1, 2023: ACS Chemical Biology
https://read.qxmd.com/read/37892658/three-dimensional-printing-and-bioprinting-in-renal-transplantation-and-regenerative-medicine-current-perspectives
#38
REVIEW
Chrysanthos D Christou, Stella Vasileiadou, Georgios Sotiroudis, Georgios Tsoulfas
For patients with end-stage kidney disease (ESKD), renal transplantation is the treatment of choice, constituting the most common solid organ transplantation. This study aims to provide a comprehensive review regarding the application of three-dimensional (3D) printing and bioprinting in renal transplantation and regenerative medicine. Specifically, we present studies where 3D-printed models were used in the training of surgeons through renal transplantation simulations, in patient education where patients acquire a higher understanding of their disease and the proposed operation, in the preoperative planning to facilitate decision-making, and in fabricating customized, tools and devices...
October 14, 2023: Journal of Clinical Medicine
https://read.qxmd.com/read/37869020/associations-between-chronic-kidney-disease-and-thinning-of-neuroretinal-layers-in-multiethnic-asian-and-white-populations
#39
JOURNAL ARTICLE
Shivani Majithia, Crystal Chun Yuen Chong, Miao Li Chee, Marco Yu, Zhi Da Soh, Sahil Thakur, Raghavan Lavanya, Tyler Hyungtaek Rim, Simon Nusinovici, Victor Koh, Charumathi Sabanayagam, Ching-Yu Cheng, Yih-Chung Tham
PURPOSE: To evaluate the relationships between chronic kidney disease (CKD) with retinal nerve fiber layer (RNFL) and ganglion cell-inner plexiform layer (GCIPL) thickness profiles of eyes in Asian and White populations. DESIGN: Cross-sectional analysis. PARTICIPANTS: A total of 5066 Asian participants (1367 Malays, 1772 Indians, and 1927 Chinese) from the Singapore Epidemiology of Eye Diseases Study (SEED) were included, consisting of 9594 eyes for peripapillary RNFL analysis and 8661 eyes for GCIPL analysis...
2024: Ophthalmol Sci
https://read.qxmd.com/read/37866804/lasmiditan-restores-mitochondrial-quality-control-mechanisms-and-accelerates-renal-recovery-after-ischemia-reperfusion-injury
#40
JOURNAL ARTICLE
Kevin A Hurtado, Jaroslav Janda, Rick G Schnellmann
BACKGROUND: Mitochondrial dysfunction is a well-established result of acute kidney injury (AKI). Previously, we identified that 5-hydroxytryptamine 1F (5-HT1F ) receptor agonism with lasmiditan induces mitochondrial biogenesis (MB) and improves renal vasculature and function in an AKI mouse model. We hypothesize that lasmiditan also modulates mitochondrial dynamics and mitophagy in a mouse model of AKI. METHODS: Male mice were subjected to renal ischemia/reperfusion (I/R) and treated daily with lasmiditan (0...
October 20, 2023: Biochemical Pharmacology
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