keyword
MENU ▼
Read by QxMD icon Read
search

Nuclear pore complex

keyword
https://www.readbyqxmd.com/read/28642229/evolutionary-dynamics-of-the-kinetochore-network-in-eukaryotes-as-revealed-by-comparative-genomics
#1
Jolien Je van Hooff, Eelco Tromer, Leny M van Wijk, Berend Snel, Geert Jpl Kops
During eukaryotic cell division, the sister chromatids of duplicated chromosomes are pulled apart by microtubules, which connect via kinetochores. The kinetochore is a multiprotein structure that links centromeres to microtubules, and that emits molecular signals in order to safeguard the equal distribution of duplicated chromosomes over daughter cells. Although microtubule-mediated chromosome segregation is evolutionary conserved, kinetochore compositions seem to have diverged. To systematically inventory kinetochore diversity and to reconstruct its evolution, we determined orthologs of 70 kinetochore proteins in 90 phylogenetically diverse eukaryotes...
June 22, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28636640/targeted-nanodiamonds-for-identification-of-subcellular-protein-assemblies-in-mammalian-cells
#2
Michael P Lake, Louis-S Bouchard
Transmission electron microscopy (TEM) can be used to successfully determine the structures of proteins. However, such studies are typically done ex situ after extraction of the protein from the cellular environment. Here we describe an application for nanodiamonds as targeted intensity contrast labels in biological TEM, using the nuclear pore complex (NPC) as a model macroassembly. We demonstrate that delivery of antibody-conjugated nanodiamonds to live mammalian cells using maltotriose-conjugated polypropylenimine dendrimers results in efficient localization of nanodiamonds to the intended cellular target...
2017: PloS One
https://www.readbyqxmd.com/read/28624666/the-nuclear-pore-complex-core-scaffold-and-permeability-barrier-variations-of-a-common-theme
#3
REVIEW
Ryo Hayama, Michael P Rout, Javier Fernandez-Martinez
The study of the nuclear pore complex (NPC) is a fascinating endeavor, as it not only implies uncovering the 'engineering marvel' of its architecture and function, but also provides a key window into a significant evolutionary event: the origin of the eukaryotic cell. The combined efforts of many groups in the field, with the help of novel methodologies and new model organisms, are facilitating a much deeper understanding of this complex assembly. Here we cover recent advances on the characterization of the structure of the NPC scaffold and of the biophysical mechanisms that define the permeability barrier...
June 15, 2017: Current Opinion in Cell Biology
https://www.readbyqxmd.com/read/28611252/the-primary-enveloped-virion-of-herpes-simplex-virus-1-its-role-in-nuclear-egress
#4
William W Newcomb, Juan Fontana, Dennis C Winkler, Naiqian Cheng, J Bernard Heymann, Alasdair C Steven
Many viruses migrate between different cellular compartments for successive stages of assembly. The HSV-1 capsid assembles in the nucleus and then transfers into the cytoplasm. First, the capsid buds through the inner nuclear membrane, becoming coated with nuclear egress complex (NEC) protein. This yields a primary enveloped virion (PEV) whose envelope fuses with the outer nuclear membrane, releasing the capsid into the cytoplasm. We investigated the associated molecular mechanisms by isolating PEVs from US3-null-infected cells and imaging them by cryo-electron microscopy and tomography...
June 13, 2017: MBio
https://www.readbyqxmd.com/read/28607146/epigenetic-transcriptional-memory-of-gal-genes-depends-on-growth-in-glucose-and-the-tup1-transcription-factor-in-saccharomyces-cerevisiae
#5
Varun Sood, Ivelisse Cajigas, Agustina D'Urso, William H Light, Jason H Brickner
Previously expressed inducible genes can remain poised for faster reactivation for multiple cell divisions, a conserved phenomenon called epigenetic transcriptional memory. The GAL genes in Saccharomyces cerevisiae show faster reactivation for up to seven generations after being repressed. During memory, previously produced Gal1 protein enhances the rate of reactivation of GAL1, GAL10, GAL2 and GAL7 These genes also interact with the nuclear pore complex (NPC) and localize to the nuclear periphery both when active and during memory...
June 12, 2017: Genetics
https://www.readbyqxmd.com/read/28602187/desi-ms-imaging-and-nmr-spectroscopy-to-investigate-the-influence-of-biodiesel-in-the-structure-of-commercial-rubbers
#6
Lorena M A Silva, Elenilson G Alves Filho, André J Simpson, Marcos R Monteiro, Elaine Cabral, Demian Ifa, Tiago Venâncio
Biodiesel has been introduced as an energetic matrix in several countries around the world. However, the affinity of biodiesel with the components of petrodiesel engines is a growing concern. In order to obtain information regarding the effect of biodiesel on the rubber structure, nuclear magnetic resonance technics under a new technology named as comprehensive multiphase (CMP NMR) and the imaging through desorption electrospray ionization mass spectrometry (DESI-MS imaging) were used. The (1)H CMP-DOSY NMR showed the entrapped fuel into the rubber cavities, which the higher constraint caused by the rubber structure is related to the smaller diffusion coefficient...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28587976/mechanosensing-of-matrix-by-stem-cells-from-contractility-and-matrix-heterogeneity-to-cardiogenesis-and-muscle-stem-cells
#7
REVIEW
Lucas Smith, Sangkyun Cho, Dennis E Discher
Stem cells are particularly 'plastic' cell types that are induced by various cues to become specialized, tissue-functional lineages by switching on the expression of specific gene programs. Matrix stiffness is among the cues that multiple stem cell types can sense and respond to. This seminar-style review focuses on mechanosensing of matrix elasticity in the differentiation or early maturation of a few illustrative stem cell types, with an intended audience of biologists and physical scientists. Contractile forces applied by a cell's acto-myosin cytoskeleton are often resisted by the extracellular matrix and transduced through adhesions and the cytoskeleton ultimately into the nucleus to modulate gene expression...
June 3, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28586646/nuclear-pores-regulate-muscle-development-and-maintenance-by-assembling-a-localized-mef2c-complex
#8
Marcela Raices, Lucas Bukata, Stephen Sakuma, Joana Borlido, Leanora S Hernandez, Daniel O Hart, Maximiliano A D'Angelo
Nuclear pore complexes (NPCs) are multiprotein channels connecting the nucleus with the cytoplasm. NPCs have been shown to have tissue-specific composition, suggesting that their function can be specialized. However, the physiological roles of NPC composition changes and their impacts on cellular processes remain unclear. Here we show that the addition of the Nup210 nucleoporin to NPCs during myoblast differentiation results in assembly of an Mef2C transcriptional complex required for efficient expression of muscle structural genes and microRNAs...
June 5, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28579449/structural-dynamics-of-the-nuclear-pore-complex
#9
REVIEW
Yusuke Sakiyama, Radhakrishnan Panatala, Roderick Y H Lim
Nuclear pore complexes (NPCs) are the sole conduits that facilitate macromolecular exchange between the nucleus and cytosol. Recent advancements have led to a more highly resolved NPC structure. However, our understanding of the NPC modus operandi that facilitates transport selectivity, and speed, of diverse cargoes remains incomplete. For the most part, assorted cargo-complexes of different sizes traverse the NPC central channel in milliseconds, yet little is known about the nanoscopic movements of its barrier-forming Phe-Gly nucleoporins (FG Nups) and related sub-structures at transport-relevant time and length scales...
June 1, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28576968/localisation-of-nup153-and-senp1-to-nuclear-pore-complexes-is-required-for-53bp1-mediated-dna-double-strand-break-repair
#10
Vincent Duheron, Nadine Nilles, Sylvia Pecenko, Valérie Martinelli, Birthe Fahrenkrog
The nuclear basket of nuclear pore complexes (NPCs) is composed of three nucleoporins: Nup153, Nup50 and Tpr. Nup153 has a role in DNA double-strand break (DSB) repair by promoting nuclear import of 53BP1, a mediator of DNA damage response. Here we provide evidence that loss of Nup153 compromises 53BP1 sumoylation, prerequisite for efficient accumulation of 53BP1 at DSBs. Depletion of Nup153 resulted in reduced SUMO1 modification of 53BP1 and the displacement of the SUMO protease SENP1 from NPCs. Artificial tethering of SENP1 to NPCs restored non-homologous end joining (NHEJ) in the absence of Nup153 and re-established 53BP1 sumoylation...
June 2, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28562428/single-nucleotide-variant-in-nucleoporin-107-may-be-predictive-of-sensitivity-to-chemotherapy-in-patients-with-ovarian-cancer
#11
Shaheen Alanee, Kristin Delfino, Andrew Wilber, Kathy Robinson, Laurent Brard, Assaad Semaan
BACKGROUND: Alterations in nuclear pore complex (NPC) genes have been previously associated with response to chemotherapy. Using agnostic exome sequencing, we envisioned that new alleles in NPC genes, predictive of sensitivity to platinum treatment, could be discovered. METHODS: Twenty-two platinum-sensitive and six platinum-resistant ovarian cancer patients were tested. Platinum sensitivity was defined as disease-free survival greater than 6 months. Next-generation sequencing of exomes was used to compare platinum-sensitive and platinum-resistant patients...
July 2017: Pharmacogenetics and Genomics
https://www.readbyqxmd.com/read/28561834/organoindium-modified-monodisperse-ellipsoid-platelet-like-periodic-mesoporous-silicas
#12
Yucang Liang, Martin Meixner
In this study, an indium(iii) silylamide complex was respectively grafted onto monodisperse ellipsoid-like (E) and platelet-like (P) large-pore hexagonal periodic mesoporous silicas (PMSs) SBA-15 to afford hybrid materials In[N(SiMe3)2]3@SBA-15E and In[N(SiMe3)2]3@SBA-15P with well-defined surface species ([triple bond, length as m-dash]SiO)2In[N(SiMe3)2], [triple bond, length as m-dash]SiOIn[N(SiMe3)2]2, and [triple bond, length as m-dash]SiOSiMe3. Surface ligand exchange between silylamido and alkoxyl group led to the conversion of surface species ([triple bond, length as m-dash]SiO)2In[N(SiMe3)2] and [triple bond, length as m-dash]SiOIn[N(SiMe3)2]2 into ([triple bond, length as m-dash]SiO)2In(OR) and [triple bond, length as m-dash]SiOIn(OR)2 (R = Me, Et, iPr) with donor ligands thf or NH3, respectively, revealing that silylamido coordinated to the indium centre could be completely exchanged with alkoxyl groups (-OMe, OEt or OiPr)...
June 13, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28560031/nuclear-roles-for-cilia-associated-proteins
#13
REVIEW
Tristan D McClure-Begley, Michael W Klymkowsky
Cilia appear to be derived, evolutionarily, from structures present in the ancestral (pre-ciliary) eukaryote, such as microtubule-based vesicle trafficking and chromosome segregation systems. Experimental observations suggest that the ciliary gate, the molecular complex that mediates the selective molecular movement between cytoplasmic and ciliary compartments, shares features with nuclear pores. Our hypothesis is that this shared transport machinery is at least partially responsible for the observation that a number of ciliary and ciliogenesis-associated proteins are found within nuclei where they play roles in the regulation of gene expression, DNA repair, and nuclear import and export...
2017: Cilia
https://www.readbyqxmd.com/read/28553638/torsin-atpases-harnessing-dynamic-instability-for-function
#14
Anna R Chase, Ethan Laudermilch, Christian Schlieker
Torsins are essential, disease-relevant AAA+ (ATPases associated with various cellular activities) proteins residing in the endoplasmic reticulum and perinuclear space, where they are implicated in a variety of cellular functions. Recently, new structural and functional details about Torsins have emerged that will have a profound influence on unraveling the precise mechanistic details of their yet-unknown mode of action in the cell. While Torsins are phylogenetically related to Clp/HSP100 proteins, they exhibit comparatively weak ATPase activities, which are tightly controlled by virtue of an active site complementation through accessory cofactors...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/28537556/the-dynamic-three-dimensional-organization-of-the-diploid-yeast-genome
#15
Seungsoo Kim, Ivan Liachko, Donna G Brickner, Kate Cook, William S Noble, Jason H Brickner, Jay Shendure, Maitreya J Dunham
The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. However, even in this well-studied model, it is unclear how homolog pairing in diploids or environmental conditions influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged Saccharomyces hybrid diploids to obtain the first global view of chromosome conformation in diploid yeasts. After controlling for the Rabl-like orientation using a polymer model, we observe significant homolog proximity that increases in saturated culture conditions...
May 24, 2017: ELife
https://www.readbyqxmd.com/read/28530826/high-speed-atomic-force-microscopy-reveals-loss-of-nuclear-pore-resilience-as-a-dying-code-in-colorectal-cancer-cells
#16
Mahmoud Shaaban Mohamed, Akiko Kobayashi, Azuma Taoka, Takahiro Watanabe-Nakayama, Yosuke Kikuchi, Masaharu Hazawa, Toshinari Minamoto, Yoshihiro Fukumori, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Richard W Wong
Nuclear pore complexes (NPCs) are the sole turnstile implanted in the nuclear envelope (NE), acting as a central nano-regulator of transport between the cytosol and the nucleus. NPCs consist of ~30 proteins, termed nucleoporins. About one-third of nucleoporins harbour natively unstructured, intrinsically disordered phenylalanine-glycine strings (FG-Nups), which engage in transport selectivity. Because the barriers insert deeply in the NPC, they are nearly inaccessible. Several in vitro barrier models have been proposed; however, the dynamic FG-Nups protein molecules themselves are imperceptible in vivo...
May 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28528776/structural-basis-of-tpr-mediated-oligomerization-and-activation-of-oncogenic-fusion-kinases
#17
Kuntal Pal, Abhishek Bandyopadhyay, X Edward Zhou, Qingping Xu, David P Marciano, Joseph S Brunzelle, Smitha Yerrum, Patrick R Griffin, George Vande Woude, Karsten Melcher, H Eric Xu
The nuclear pore complex subunit TPR is found in at least five different oncogenic fusion kinases, including TPR-MET, yet how TPR fusions promote activation of kinases and their oncogenic activities remains poorly understood. Here we report the crystal structure of TPR(2-142), the MET fusion partner of oncogenic TPR-MET. TPR(2-142) contains a continuous 124-residue α helix that forms an antiparallel tetramer from two leucine zipper-containing parallel coiled coils. Remarkably, single mutations cause strikingly different conformations of the coiled coil, indicating its highly dynamic nature...
June 6, 2017: Structure
https://www.readbyqxmd.com/read/28515232/the-nuclear-export-factor-crm1-controls-juxta-nuclear-microtubule-dependent-virus-transport
#18
I-Hsuan Wang, Christoph J Burckhardt, Artur Yakimovich, Matthias K Morf, Urs F Greber
Transport of large cargo through the cytoplasm requires motor proteins and polarized filaments. Viruses that replicate in the nucleus of post-mitotic cells use microtubules and the dynein/dynactin motor to traffic to the nuclear membrane, and deliver their genome through nuclear pore complexes (NPCs) into the nucleus. How virus particles (virions) or cellular cargo are transferred from microtubules to the NPC is unknown. Here, we analyzed trafficking of incoming cytoplasmic adenoviruses by single particle tracking and super-resolution microscopy...
May 17, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28506894/the-functional-versatility-of-the-nuclear-pore-complex-proteins
#19
REVIEW
Mohammed Hezwani, Birthe Fahrenkrog
Over the past few decades, it is increasingly evident that nucleoporins are multi-functional proteins that are not only pivotal for the formation of the nuclear pore complex. They also have key roles in mitosis, gene expression, development and disease. In this review, the versatility and functions of nucleoporins outside the NPC will be discussed.
May 12, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28506892/the-roles-of-the-nuclear-pore-complex-in-cellular-dysfunction-aging-and-disease
#20
REVIEW
Stephen Sakuma, Maximiliano A D'Angelo
The study of the Nuclear Pore Complex (NPC), the proteins that compose it (nucleoporins), and the nucleocytoplasmic transport that it controls have revealed an unexpected layer to pathogenic disease onset and progression. Recent advances in the study of the regulation of NPC composition and function suggest that the precise control of this structure is necessary to prevent diseases from arising or progressing. Here we discuss the role of nucleoporins in a diverse set of diseases, many of which directly or indirectly increase in occurrence and severity as we age, and often shorten the human lifespan...
May 12, 2017: Seminars in Cell & Developmental Biology
keyword
keyword
88873
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"