Xiaohan Yang, Timothy J Tschaplinski, Gregory B Hurst, Sara Jawdy, Paul E Abraham, Patricia K Lankford, Rachel M Adams, Manesh B Shah, Robert L Hettich, Erika Lindquist, Udaya C Kalluri, Lee E Gunter, Christa Pennacchio, Gerald A Tuskan
Small proteins (10-200 amino acids [aa] in length) encoded by short open reading frames (sORF) play important regulatory roles in various biological processes, including tumor progression, stress response, flowering, and hormone signaling. However, ab initio discovery of small proteins has been relatively overlooked. Recent advances in deep transcriptome sequencing make it possible to efficiently identify sORFs at the genome level. In this study, we obtained ~2.6 million expressed sequence tag (EST) reads from Populus deltoides leaf transcriptome and reconstructed full-length transcripts from the EST sequences...
April 2011: Genome Research