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miRNA prediction

L-J Hou, J-J Han, Y Liu
OBJECTIVE: The present study is aimed to investigate the regulatory effect of microRNA (miRNA or miR)-503 on endothelial functions, as well as the mechanism by which high glucose leads to injury of endothelial cells. MATERIALS AND METHODS: When reaching 80% confluency, human umbilical vein endothelial cells (HUVECs) were subjected to non-serum synchronization for 12 h, and medium of cells in high-glucose (HG) group was replaced by normal medium supplemented with 25 mmol/L D-glucose...
June 2018: European Review for Medical and Pharmacological Sciences
Bo Wang, Mujun Yin, Cheng Cheng, Hongpeng Jiang, Kewei Jiang, Zhanlong Shen, Yingjiang Ye, Shan Wang
Growing evidence indicates a potential role for miR‑490‑3p in tumorigenesis. However, its function in colorectal carcinoma (CRC) remains undefined. In this study, miR‑490‑3p was markedly downregulated in fifty colorectal cancer tissue samples compared with the corresponding adjacent non‑cancerous specimens, by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression levels of miR‑490‑3p were closely associated with tumor differentiation and distant metastasis. In addition, both Kaplan-Meier and multivariate analyses indicated CRC patients with elevated miR‑490‑3p amounts had prolonged overall survival...
June 19, 2018: International Journal of Oncology
Yaozong Wang, Jianjun Li, Lei Dai, Jueru Zheng, Zhanbo Yi, Liangliang Chen
BACKGROUND: Recurrence following conventional therapies in patients with breast cancer is a major cause of morbidity and mortality. This study aimed to investigate potential predictive biomarkers for breast cancer recurrence especially microRNAs (miRNAs). METHODS: The primary breast cancer patients who were scheduled to undergo curative resection in our hospital from May 2007 to May 2012 were recruited in this study. Clinical and pathological characteristics were compared in patients with or without recurrence...
June 5, 2018: Cancer Biomarkers: Section A of Disease Markers
Xin Zhou, Zipeng Lu, Tongshan Wang, Zebo Huang, Wei Zhu, Yi Miao
The differential expression of microRNAs (miRNAs) in plasma of pancreatic cancer (PC) patients may act as a diagnostic biomarker. A four-stage study was performed to identify plasma miRNAs with potential in detecting PC. Exiqon panels (20 PC vs. 10 normal controls (NCs)) were applied in the screening phase to obtain miRNA profiling. The identified miRNAs were further assessed in the training (40 PC vs. 40 NCs) and testing stages (112 PC vs. 116 NCs) with qRT-PCR assays. A six-miRNA signature including up-regulated miR-122-5p, miR-125b-5p, miR-192-5p, miR-193b-3p, miR-221-3p and miR-27b-3p was identified...
June 15, 2018: Gene
Minhao Yu, Yang Luo, Zhijie Cong, Yifei Mu, Yier Qiu, Ming Zhong
Background/Aims: Hippo signaling is an evolutionarily conserved pathway that controls organ size by regulating cell proliferation, survival, apoptosis, and stem cell self-renewal. In addition, Hippo signaling is profoundly implicated in intestinal regeneration and cancer. However, its roles in the pathogenesis of Crohn's disease (CD) remain largely unexplored. Methods: Quantitative real-time PCR was performed to identify the deregulated molecules in Hippo signaling...
April 18, 2018: Journal of Crohn's & Colitis
Markus Bookland, Min Tang-Schomer, Eileen Gillan, Antonina Kolmakova
BACKGROUND: Juvenile pilocytic astrocytomas represent the largest group of pediatric brain tumors. The ideal management for these tumors is early, total surgical resection. To detect and track treatment response, a screening tool is needed to identify patients for surgical evaluation and assess the quality of treatment. The identification of aberrant miRNA profiles in the sera of juvenile pilocytic astrocytoma patients could provide such a screening tool. METHODS: The authors reviewed the serum profiles of 84 oncologically relevant miRNAs in pediatric juvenile pilocytic astrocytoma patients via qPCR screening...
June 18, 2018: Acta Neurochirurgica
Heng Liu, Yin Hu, Bin Zhuang, Jing Yin, Xiao-Hui Chen, Juan Wang, Meng-Meng Li, Jing Xu, Xing-Yun Wang, Zhang-Bin Yu, Shu-Ping Han
Objectives: To explore and validate the differential expression of circRNAs in the myocardium of congenital ventricular septal defect (VSD) and to explore a new avenue of research regarding the pathological mechanisms of VSD. Methods: We detected circRNAs expression profiles in heart tissues taken from six aborted fetuses with VSD and normal group using circRNA microarray. Some differentially expressed circRNAs were studied by bioinformatics analysis. Finally, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results...
2018: International Journal of Medical Sciences
Marco Siano, Vittoria Espeli, Nicolas Mach, Paolo Bossi, Lisa Licitra, Michele Ghielmini, Milo Frattini, Silvana Canevari, Loris De Cecco
OBJECTIVE: Platinum-based chemotherapy plus the anti-EGFR monoclonal antibody (mAb) cetuximab is used to treat recurrent/metastatic (RM) head-neck squamous cell carcinoma (HNSCC). Recently, we defined Cluster3 gene-expression signature as a potential predictor of favorable progression-free survival (PFS) in cetuximab-treated RM-HNSCC patients and predictor of partial metabolic FDG-PET response in an afatinib window-of-opportunity trial. Another anti-EGFR-mAb (panitumumab) was used as the treatment agent in RM-HNSCC patients in the phase II PANI01trial...
July 2018: Oral Oncology
Alieh Gholaminejad, Hossein Abdul Tehrani, Mohammad Gholami Fesharaki
CONTEXT: The prognostic, diagnostic and therapeutic value of microRNA (miRNA) expression aberrations in renal fibrosis has been studied in recent years. However, the miRNA expression profiling efforts have led to inconsistent results between the studies. OBJECTIVE(S): The aim of this study was to perform a meta-analysis on the renal fibrosis miRNA expression profiling studies to identify candidate diagnostic biomarkers. MATERIALS AND METHODS: We performed comprehensive literature searches in several databases to identify miRNA expression studies of renal fibrosis in animal models and humans...
June 18, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Gregory J Weber, Jaleyea Foster, Sathnur B Pushpakumar, Utpal Sen
Hypertension affects nearly one third of the adult US population and is a significant risk factor for chronic kidney disease (CKD). An expanding body of recent studies indicates that gut microbiome has crucial roles in regulating physiological processes through, among other mechanisms, one mode of short chain fatty acids (SCFA) and their target receptors. In addition, these SCFA receptors are potential targets of regulation by host miRNAs, however, the mechanisms through which this occurs is not clearly defined...
June 14, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Huiyuan Hou, Fan Gao, Hongliang Liang, Yang Lv, Manhong Li, Libo Yao, Jian Zhang, Guorui Dou, Yusheng Wang
Our previous investigations have shown that bone marrow-derived cells (BMCs), including mesenchymal stem cells (MSCs), contribute to the development of choroidal neovascularization (CNV) as sources of cells and angiogenic factors. Two main steps for circulating BMCs to integrate into CNV lesions are extracellular matrix remodeling and consequential cell migration. MicroRNAs (miRNAs) were found to be involved in CNV development; however, little is known about whether miRNAs regulate the contribution of BMCs to CNV...
June 14, 2018: Experimental Eye Research
Jenna Kropp Schmidt, Lindsey N Block, Thaddeus G Golos
OBJECTIVE: Expression of microRNAs (miRNAs) in the human placenta is dynamic across gestation, with expression of miRNAs belonging to the C14MC, C19MC and miR-371-3 clusters. Specifically, miRNAs within the C19MC cluster are exclusively expressed in primates with predominant expression in the placenta. Non-human primates can be utilized to study developmental processes of placentation in vivo that cannot be assessed in the human placenta, however, miRNA expression has not been defined in the macaque placenta...
May 2018: Placenta
Zengkui Lu, Lixin Du, Ruizao Liu, Ran Di, Liping Zhang, Youji Ma, Qing Li, Enmin Liu, Mingxing Chu, Caihong Wei
MicroRNA (miRNA) is a sort of endogenous ~20-25 nt non-coding RNAs, and it can regulate a variety of biological events. We found the miR-378 may involve in regulating the muscle development of sheep during our previous research. However, the molecular mechanism of miR-378 regulating myoblast proliferation is still unclear. In this research, we predicted that BMP2 (Bone morphogenetic protein 2) was the target gene of miR-378 and the BMP-Smad signal pathway that BMP2 participated in playing an important role in the muscle development...
June 13, 2018: Gene
Shigang Tian, Yuan Yuan, Zhuo Li, Ming Gao, Ying Lu, Haiying Gao
BACKGROUND: Recent studies have shown that the long non-coding RNA urothelial carcinoma-associated (UCA1) plays a key role in cardiovascular injury. However, the potential biological role of UCA1 in progression of atherosclerosis (AS) remains unclear. The aim of the present study is to identify the regulation of lncRNA UCA1 on atherosclerosis-related vascular dysfunction via miR-26a targeting phosphatase and tensin homolog (PTEN), and investigate the underlying mechanisms in the development of atherosclerosis...
June 13, 2018: Gene
Yingjie Shao, Dachuan Zhang, Xiaodong Li, Jing Yang, Lujun Chen, Zhonghua Ning, Yun Xu, Guohua Deng, Min Tao, Yibei Zhu, Jingting Jiang
BACKGROUND: It has been reported that B-cell-specific moloney leukemia virus insertion site 1 (Bmi-1) has important roles in various cancers, but its regulation through microRNAs (miRNAs) and its functions in hepatocellular carcinoma (HCC) remains unclear. METHODS: We investigated Bmi-1 expression and its prognostic significance in HCC by using tissue samples and The Cancer Genome Atlas (TCGA) datasets. The relationship between miRNAs and Bmi-1 was verified by bioinformatics prediction and immunofluorescence...
June 15, 2018: Molecular Pharmaceutics
Jing Wang, Shuzhi Wu, Tianren Huang
The aim of the present study was to examine the expression and role of vascular endothelial growth factor A (VEGFA) and microRNA (miRNA or miR)-381 in tumor thrombi from patients with hepatocellular carcinoma and portal vein tumor thrombus (PVTT). Tumor thrombi and adjacent paired tissues were collected from 39 patients with hepatocellular carcinoma with PVTT. VEGFA expression levels were assessed using reverse transcription-quantitative polymerase chain reaction and western blotting. miRNAs that may regulate VEGFA expression were predicted using bioinformatics analysis and confirmed via a dual luciferase reporter assay...
June 2018: Experimental and Therapeutic Medicine
Wendao You, Xing Zhang, Mengyue Ji, Yang Yu, Chen Chen, Yujia Xiong, Yiting Liu, Yibin Sun, Chenhuan Tan, Hanshuo Zhang, Jie Li, Weichang Chen, Rui Li
Gastric cancer (GC) is one of the most common malignancies with high mortality rate. MiR-152 may exert the function of tumor suppressor by regulating its target gene, including PIK3CA. Nevertheless, all of the described functions are referred explicitly to miR-152-3p, while miR-152-5p as a passenger strand is poorly realized and entirely ignored. We previously selected miR-152-5p as a candidate using cell migration inhibition screening for GC cells and predicted that miR-152-5p might also target PIK3CA. In this study, we found an abnormal proportion of miR-152-3p / miR-152-5p in GC (gastric cancer) tissues and cells and demonstrated that miR-152-5p had poorer stability in GC cells, revealing the possibility that miR-152-5p is abnormally "suppressed" in gastric cancer...
2018: International Journal of Biological Sciences
Tao Zhang, Kangfeng Jiang, Xinying Zhu, Gan Zhao, Haichong Wu, Ganzhen Deng, Changwei Qiu
Breast cancer is one of the most lethal cancers in the world. The fight against breast cancer has also become a major task for medical workers. MicroRNAs (miRNAs) are often aberrantly expressed in diverse cancers and are involved in progression and metastasis. Many studies have found that miRNAs can act as oncogenes or as tumor suppressor genes. Here, we show that miR-433 is significantly decreased in breast cancer cells. In addition, we demonstrate the effects of miR-433 on breast cancer cell apoptosis, migration and proliferation in an attempt to elucidate the mechanism of action of miR-433...
2018: International Journal of Biological Sciences
Rikke Karlin Jepsen, Guy Wayne Novotny, Louise Laurberg Klarskov, Claus Heiner Bang-Bertelsen, Ida Trondhjem Haakansson, Anker Hansen, Ib Jarle Christensen, Lene Buhl Riis Ass, Estrid Høgdall
Accurate prediction of regional lymph node metastases (LNM) in endoscopically resected pT1 colorectal cancer (CRC) is crucial in treatment stratification for subsequent radical surgery. Several miRNAs have been linked to CRC invasion and metastasis, including the oncogenic miR-17/92 cluster, and expression levels might have predictive value in the risk assessment of early metastatic progression in CRC. We performed global miRNA microarray using tissue samples from the invasive front of pT1 CRC and investigated associations of the miR-17/92 cluster and presence of LNM...
June 11, 2018: Human Pathology
Xiaoying Wu, Xiaojun Zhao, Xingyu Miao
Accumulating evidence has documented that microRNAs (miRNAs) are critical regulators of neural stem cell (NSC) proliferation and differentiation. MiRNA-374b (miR-374b) has been reported to play an important role in regulating various cellular processes, such as proliferation and differentiation. However, whether miR-374b is involved in NSC proliferation and differentiation remains unclear. In this study, we investigated the potential role of miR-374b in regulating NSC proliferation and differentiation to elucidate the underlying molecular mechanism...
June 11, 2018: Biochemical and Biophysical Research Communications
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