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https://www.readbyqxmd.com/read/28634713/characterization-and-in-vitro-antibacterial-activity-of-saponin-conjugated-silver-nanoparticles-against-bacteria-that-cause-burn-wound-infection
#1
Anbarasan Muniyan, Kalaiselvi Ravi, Udayamathi Mohan, Rajasekar Panchamoorthy
Saponins are glycosides, which destabilize the membrane by altering the membrane permeability. Thus, the present study was aimed to fabricate the silver nanoparticles (AgNPs) using fenugreek derived saponin (SN) against multi-drug resistant bacteria. This study has optimized the concentration of SN (240 mg/mL) for the synthesis of an effective AgNPs against the test organism Escherichia coli. The SN reduced AgNPs produced a reddish-brown colour and displayed UV absorption at 416 nm. The bio-reducing efficiency of SN (62...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28629938/poly-glycidyl-methacrylate-nanoparticle-coated-capillary-with-oriented-antibody-immobilization-for-immunoaffinity-in-tube-solid-phase-microextraction-preparation-and-characterization
#2
Ling-Ling Ying, Yuan-Chun Ma, Bei Xu, Xian-Hua Wang, Lin-Yi Dong, Dong-Mei Wang, Kun Liu, Liang Xu
A combination between modification with nanoparticles (NP) and oriented antibody immobilization (OAI) on the inner face of capillary was for the first time developed for immunoaffinity in-tube solid-phase microextraction (SPME) to promise high antigen extraction capacity. β2-microglobin (β2MG) and cystatin C (Cys-C) were selected as model antigens. Poly(glycidyl methacrylate) (PGMA) NPs were chemically immobilized onto the capillary by a ring-opening reaction. Antibodies for β2MG and Cys-C were immobilized on the NPs through OAI...
June 12, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28629050/novel-bilayer-wound-dressing-based-on-electrospun-gelatin-keratin-nanofibrous-mats-for-skin-wound-repair
#3
Chun-Hsu Yao, Chia-Yu Lee, Chiung-Hua Huang, Yueh-Sheng Chen, Kuo-Yu Chen
A bilayer membrane (GKU) with a commercial polyurethane wound dressing as an outer layer and electrospun gelatin/keratin nanofibrous mat as an inner layer was fabricated as a novel wound dressing. Scanning electron micrographs showed that gelatin/keratin nanofibers had a uniform morphology and bead-free structure with average fiber diameter of 160.4nm. 3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide assay using L929 fibroblast cells indicated that the residues released from the gelatin/keratin composite nanofibrous mat accelerated cell proliferation...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629008/multi-walled-carbon-nanotubes-graphene-oxide-hybrid-and-nanohydroxyapatite-composite-a-novel-coating-to-prevent-dentin-erosion
#4
Sídnei Nahorny, Hudson Zanin, Vinie Abreu Christino, Fernanda Roberta Marciano, Anderson Oliveira Lobo, Luís Eduardo Silva Soares
To date is emergent the development of novel coatings to protect erosion, especially to preventive dentistry and restorative dentistry. Here, for the first time we report the effectiveness of multi-walled carbon nanotube/graphene oxide hybrid carbon-base material (MWCNTO-GO) combined with nanohydroxyapatite (nHAp) as a protective coating for dentin erosion. Fourier transform Raman spectroscopy (FT-Raman), scanning electron (SEM), and transmission electron (TEM) microscopy were used to investigated the coatings and the effect of acidulated phosphate fluoride gel (APF) treatment on bovine teeth root dentin before and after erosion...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629004/performance-evaluation-of-non-ionic-surfactant-based-tazarotene-encapsulated-proniosomal-gel-for-the-treatment-of-psoriasis
#5
Vure Prasad, Sundeep Chaurasia
The study aims to explore the potential of non-ionic surfactant based proniosomal gel (PNG) in improving the topical delivery of tazarotene by in vitro and in vivo studies. The PNG was prepared using coacervation phase separation method composed of span, stearylamine, cholesterol, and lecithin. The PNG demonstrated favorable vesicle size (3.26±0.22μm) and percent encapsulation efficiency (49.50±2.3%). The PNG was evaluated for viscosity which indicated that the ratio of span:cholesterol:stearylamine (64...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628682/radiation-therapy-in-dermatology-non-melanoma-skin-cancer
#6
Nikoo Cheraghi, Armand Cognetta, David Goldberg
<p>Background: Dermatologists were historically well versed in the use of radiation therapy for the management of non-melanoma skin cancers and various inflammatory dermatologic conditions. With the advent of Mohs micrographic surgery and therapeutic discoveries for treating inflammatory dermatoses, radiotherapy assumed loss of a role in the clinical repertoire of the dermatologist. In recent years, its importance has again been realized for the management of non-melanoma skin cancers not amenable to surgical treatment or as adjuvant or palliative therapy...
May 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28627552/graphite-exfoliation-in-cellulose-solutions
#7
E S Ferreira, D S da Silva, T A L Burgo, B C Batista, F Galembeck
Shaking graphite powder dispersed in an aqueous alkaline cellulose solution produces stable dispersions of hydrophilic, thin graphite sheets with lateral dimensions reaching many micrometers. The X-ray diffractogram and Raman spectrum of the exfoliated graphite differ from the well-known graphite patterns. Analytical transmission electron micrographs show cellulose bound to the surface of thin lamellae and this is confirmed by scanning probe micrographs. The dispersant properties of dissolved cellulose are assigned to its adsorption on graphite by juxtaposition of the hydrophobic planes on both substances, forming hydrophilic particles...
June 19, 2017: Nanoscale
https://www.readbyqxmd.com/read/28620619/effects-of-1e-4e-2-methyl-1-5-bis-4-nitrophenyl-penta-1-4-dien-3-one-on-trypanosoma-cruzi-and-its-combinational-effect-with-benznidazole-ketoconazole-or-fluconazole
#8
Francieli Peron, Danielle Lazarin-Bidóia, Zia Ud Din, Edson Rodrigues-Filho, Tânia Ueda-Nakamura, Sueli de Oliveira Silva, Celso Vataru Nakamura
This study reports the activity induced by (1E,4E)-2-methyl-1,5-bis(4-nitrophenyl)penta-1,4-dien-3-one (A3K2A3) against Trypanosoma cruzi. This compound showed trypanocidal activity against the multiplicative epimastigote and amastigote forms of this protozoan, with IC50 values of 1.99 ± 0.17 and 1.20 ± 0.16 μM, respectively, and EC50 value of 15.57 ± 0.34 μM against trypomastigotes. The combination of A3K2A3 with benznidazole or ketoconazole demonstrated strong synergism, increasing effectiveness against trypomastigotes or epimastigotes of T...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28620549/lamination-of-microfibrous-plga-fabric-by-electrospinning-a-layer-of-collagen-hydroxyapatite-composite-nanofibers-for-bone-tissue-engineering
#9
Gi-Wan Kwon, Kailash Chandra Gupta, Kyung-Hye Jung, Inn-Kyu Kang
BACKGROUND: To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen-hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination of collagen-hydroxyapatite composite nanofibers on polydopamine-coated microfibrous PLGA fabrics was carried through electrospinning the solution of collagen containing L-glutamic acid-grafted hydroxyapatite nanorods (nHA-GA) at a flow rate of 1...
2017: Biomaterials Research
https://www.readbyqxmd.com/read/28619614/rapid-ex-vivo-examination-of-mohs-specimens-using-optical-coherence-tomography
#10
D Rashed, D Shah, A Freeman, R J Cook, C Hopper, C M Perrett
BACKGROUND: Mohs micrographic surgery (MMS) is an effective treatment for certain non-melanoma skin cancers. Optical coherence tomography (OCT) is a biomedical imaging modality that permits high-resolution imaging of the epidermis and dermis with the potential to detect both healthy tissue and tumour. OCT may also provide a means of detecting and differentiating between the various histological subtypes of basal cell carcinomas (BCC) in vivo. OBJECTIVE: The aim of this prospective ex vivo study was to evaluate the efficacy of OCT in recognising healthy and pathological margins of excised BCC lesions and detecting different BCC subtypes...
June 12, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28609857/new-records-of-millipedes-and-centipedes-from-bulgaria-with-an-annotated-checklist-of-the-bulgarian-myriapods
#11
Darina Bachvarova, Boyan Vagalinski, Aleksandar Doichinov, Pavel Stoev
The present paper records new and poorly known myriapods (Diplopoda, Chilopoda) collected in Bulgaria in the last 10 years. Four new species are reported as new to the Bulgarian fauna: Lithobius melanops Newport, 1845 (Chilopoda: Lithobiomorpha: Lithodiidae), Polydesmus collaris C.L. Koch, 1847, Polydesmus inconstans Latzel, 1884 and Polydesmus schaessburgensis Verhoeff, 1898 (Diplopoda: Polydesmida: Polydesmidae). Scanning electron micrographs of the gonopods and male leg-pairs 5 and 6 are provided for the first time for Bulgarosoma superficiei Strasser, 1975, a species hitherto known only from its original description...
May 10, 2017: Zootaxa
https://www.readbyqxmd.com/read/28607608/clinical-features-pathological-findings-and-treatment-of-recurrent-dermatofibrosarcoma-protuberans
#12
Yanan Li, Chuan Wang, Bo Xiang, Siyuan Chen, Li Li, Yi Ji
Dermatofibrosarcoma protuberans (DESP) is a relatively rare and low-grade tumor of the skin and soft tissue. We review the clinical features, pathological findings and management of recurrent DESP with the aim to improve our understanding of this rare tumor. Fifty-seven patients were diagnosed with recurrent DFSP. The mean age at the presentation of DFSP was 30.9 years. The mean age at diagnosis of DFSP was 40.2 years. Chest wall was the dominant location. The histopathological diagnosis was ordinary DFSP (O-DFSP) in 46 cases and fibrosarcomatous DFSP (FS-DFSP) in 11 cases...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28606530/conversion-of-cellulose-into-reducing-sugar-by-solution-plasma-process-spp
#13
I Prasertsung, P Chutinate, A Watthanaphanit, N Saito, S Damrongsakkul
In the present study, cellulose colloids are treated with the solution plasma process in order to prepare reducing sugar. The investigated parameters are treatment time, type of electrodes, and applied pulse frequency of the bipolar supply. The reducing sugar was characterized by DNS method and the%yield of total reducing sugar (TRS) was then calculated. The crystal structure and chemical structure of plasma-treated cellulose was measured by XRD and FT-IR, respectively. The%yield of TRS was greatly enhanced by solution plasma treatment using Fe electrode...
September 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28603130/fluconazole-and-its-interaction-with-metal-ii-complexes-sem-spectroscopic-and-antifungal-studies
#14
Mohsin Ali, Mansoor Ahmed, Shakil Ahmed, Syed Imran Ali, Samina Perveen, Majid Mumtaz, Syed Moazzam Haider, Urooj Nazim
The human digestive tract contains some 100 trillion cells and thousands of species of micro-organisms may be present as normal flora of this tract as well as other mucocutaneous junctions of the body. Candida specie is the most common organism residing in these areas and can easily invade the internal tissues in cases of loss of host defenses. Modifications of previously existing antifungal agents may provide new options to fight against these species. Inorganic compounds of different antifungals are under investigations...
January 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28602985/comparative-studies-of-tripolyphosphate-and-glutaraldehyde-cross-linked-chitosan-botanical-pesticide-nanoparticles-and-their-agricultural-applications
#15
Michael Gabriel Paulraj, Savarimuthu Ignacimuthu, Munusamy Rajiv Gandhi, Azeez Shajahan, Pathalam Ganesan, Soosaimanickam Maria Packiam, Naif Abdhullah Al-Dhabi
A nanopesticide formulation was developed using chitosan and a botanical pesticide PONNEEM(®) and its antifeedant, larvicidal and growth regulating activities were screened against Helicoverpa armigera, a major lepidopteran pest. Chitosan nanoparticles (CSNs) were prepared by using two different cross-linking agents namely glutaraldehyde (GLA) and tripolyphosphate (TPP). The effects of cross linking agents on CSNs and the biological properties against the insect pest were also studied. Cross linking of chitosan with either TPP or GLA was confirmed through Fourier Transform Infrared (FTIR) spectroscopy...
June 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28595252/recurrent-tumors-referred-for-mohs-micrographic-surgery-a-12-year-experience-at-a-single-academic-center
#16
H William Higgins, Sara Capobianco, Kachiu C Lee
BACKGROUND: Mohs micrographic surgery (MMS) is used for treatment of primary and recurrent tumors. Compared with primary tumors, recurrent tumors are often more aggressive. OBJECTIVE: To understand differing characteristics between primary versus recurrent tumors treated by MMS. MATERIALS AND METHODS: The authors conducted a retrospective review of a 12-year period at 1 academic center. Recurrent tumors were defined as recurrent if previously treated with cryotherapy, topical chemotherapeutics, electrodesiccation and curettage, or excision...
June 6, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28595243/determinants-and-timeline-of-perioperative-anxiety-in-mohs-surgery
#17
Irèn Kossintseva, David Zloty
BACKGROUND: Patients undergoing Mohs micrographic surgery (MMS) exhibit anxiety relating to cancer cure or the expected cosmetic outcome. OBJECTIVE: To obtain quantitative measurements of perioperative cancer and cosmetic anxiety levels in first-time MMS patients. Parameters influencing anxiety and its natural course were assessed. METHODS: Prospective, single-blinded, questionnaire study of 173 patients undergoing MMS of the face. Anxiety levels were assessed using a visual analog scale preoperatively and postoperatively over 6 months...
June 6, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28595135/soft-nanocomposites-of-gelatin-and-poly-3-hydroxybutyrate-nanoparticles-for-dual-drug-release
#18
Rafael A Bini, Mônica F Silva, Laudemir C Varanda, Marcelo A da Silva, Cécile A Dreiss
We developed a nanocomposite gel composed of gelatin and poly(3-hydroxybutyrate) polymeric nanoparticles (PNP) to be used as an injectable gel for the contemporaneous, dual sustained release of bioactive molecules. The hydrogel matrix was formed by a very simple process, using either the physical gelation of gelatin or the natural enzyme transglutaminase to covalently cross-link the gelatin chains in the presence of embedded PNP. Oscillatory rheological measurements showed that the addition of the PNP induced an increase in the storage modulus compared to pure gelatin gels, for both physical and chemical gels...
May 22, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28594895/atp-sensitive-potassium-katp-channel-openers-diazoxide-and-nicorandil-lower-intraocular-pressure-by-activating-the-erk1-2-signaling-pathway
#19
Uttio Roy Chowdhury, Cindy K Bahler, Bradley H Holman, Michael P Fautsch
Elevated intraocular pressure is the most prevalent and only treatable risk factor for glaucoma, a degenerative disease of the optic nerve. While treatment options to slow disease progression are available, all current therapeutic and surgical treatments have unwanted side effects or limited efficacy, resulting in the need to identify new options. Previous reports from our laboratory have established a novel ocular hypotensive effect of ATP-sensitive potassium channel (KATP) openers including diazoxide (DZ) and nicorandil (NCD)...
2017: PloS One
https://www.readbyqxmd.com/read/28584491/evaluation-of-cassia-tora-linn-against-oxidative-stress-induced-dna-and-cell-membrane-damage
#20
R Sunil Kumar, Ramesh Balenahalli Narasingappa, Chandrashekar G Joshi, Talakatta K Girish, Ummiti Js Prasada Rao, Ananda Danagoudar
OBJECTIVE: The present study aims to evaluate antioxidants and protective role of Cassia tora Linn. against oxidative stress-induced DNA and cell membrane damage. MATERIALS AND METHODS: The total and profiles of flavonoids were identified and quantified through reversed-phase high-performance liquid chromatography. In vitro antioxidant activity was determined using standard antioxidant assays. The protective role of C. tora extracts against oxidative stress-induced DNA and cell membrane damage was examined by electrophoretic and scanning electron microscopic studies, respectively...
January 2017: Journal of Pharmacy & Bioallied Sciences
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