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https://www.readbyqxmd.com/read/27909970/over-expression-of-mir158-causes-pollen-abortion-in-brassica-campestris-ssp-chinensis
#1
Zhiming Ma, Jianxia Jiang, Ziwei Hu, Tianqi Lyu, Yang Yang, Jingjing Jiang, Jiashu Cao
We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen contents from the binucleate microspore stage. These results first suggest the role of miR158 in pollen development of Brassica campestris ssp. chinensis. MicroRNAs (miRNAs) play crucial roles in many important growth and development processes both in plants and animals by regulating the expression of their target genes via mRNA cleavage or translational repression...
December 1, 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/27903078/why-prevention-of-repeat-abortion-is-so-challenging-psychosocial-characteristics-of-women-at-risk
#2
Brigitte Leeners, Simone Bieli, Dorothy Huang, Sibil Tschudin
OBJECTIVES: Abortion rates have declined in recent decades; however, the rate of repeat abortion remains high. In order to identify keys to making efficient interventions against repeat abortion, our objectives were: to assess the percentage of repeat abortions in women opting for termination of pregnancy over a period of 1 year; to identify the risk factors for repeat abortion; and to assess the characteristics of women who opted for a further pregnancy termination despite having received standard post-abortion care...
November 30, 2016: European Journal of Contraception & Reproductive Health Care
https://www.readbyqxmd.com/read/27899410/-repeat-abortion-a-phrase-to-be-avoided-qualitative-insights-into-labelling-and-stigma
#3
Lesley Hoggart, Victoria Louise Newton, Louise Bury
BACKGROUND: In recent years there has been growing international interest in identifying risk factors associated with 'repeat abortion', and developing public health initiatives that might reduce the rate. This article draws on a research study looking at young women's abortion experience in England and Wales. The study was commissioned with a specific focus on women who had undergone more than one abortion. We examine what may influence women's post-abortion reproductive behaviour, in addition to exploring abortion-related stigma, in the light of participants' own narratives...
November 29, 2016: Journal of Family Planning and Reproductive Health Care
https://www.readbyqxmd.com/read/27818118/-medical-induced-abortion
#4
K Bettahar, A Pinton, T Boisramé, V Cavillon, S Wylomanski, I Nisand, D Hassoun
OBJECTIVE: Updated clinical recommendations for medical induced abortion procedure. METHODS: A systematic review of French and English literature, reviewing the evidence relating to the provision of medical induced abortion was carried out on PubMed, Cochrane Library and international scientific societies recommendations. RESULTS: The effectiveness of medical abortion is higher than 95% when the protocols are adjusted to gestational age (EL1)...
November 3, 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/27803774/same-site-submucosal-tunneling-for-a-repeat-per-oral-endoscopic-myotomy-a-safe-and-feasible-option
#5
Antonios N Wehbeh, Parit Mekaroonkamol, Qiang Cai
Per oral endoscopic myotomy (POEM) is a novel endoscopic procedure for achalasia treatment. Due to its novelty and high success rates, a repeat procedure is usually not warranted, making the feasibility and safety of such approach unknown. We report the first case of a successful repeat POEM done at the same site of a previously uncompleted POEM. An 84-year-old female with type 2 achalasia presented for a POEM procedure. The procedure was aborted at the end of tunneling and before myotomy due to hypotension, which later resolved spontaneously...
October 16, 2016: World Journal of Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/27779816/key-concepts-in-pregnancy-of-unknown-location-identifying-ectopic-pregnancy-and-providing-patient-centered-care
#6
REVIEW
Loren Fields, Alison Hathaway
Pregnancy of unknown location (PUL) is a descriptive term for when a woman with a positive pregnancy test has a transvaginal ultrasound that cannot determine the site of the pregnancy. While the majority of women with PUL are subsequently diagnosed with a spontaneous abortion or viable intrauterine pregnancy, 7% to 20% of these women have an ectopic pregnancy. The potential for morbidity and mortality related to an ectopic pregnancy means that considerable care is necessary in the evaluation and management of women with PUL...
October 25, 2016: Journal of Midwifery & Women's Health
https://www.readbyqxmd.com/read/27773547/-post-abortion-contraception
#7
A Ohannessian, C Jamin
OBJECTIVE: To establish guidelines of the French National College of Gynecologists and Obstetricians about post-abortion contraception. MATERIALS AND METHODS: A systematic review of the literature about post-abortion contraception was performed on Medline and Cochrane Database between 1978 and March 2016. The guidelines of the French and foreign scientific societies were also consulted. RESULTS AND DISCUSSION: After an abortion, if the woman wishes to use a contraception, it should be started as soon as possible because of the very early ovulation resumption...
October 20, 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/27756611/a-multicenter-international-registry-of-redo-per-oral-endoscopic-myotomy-poem-after-failed-poem
#8
Amy Tyberg, Stefan Seewald, Reem Z Sharaiha, Guadalupe Martinez, Amit P Desai, Nikhil A Kumta, Arnon Lambroza, Amrita Sethi, Kevin M Reavis, Ketisha DeRoche, Monica Gaidhane, Michael Talbot, Payal Saxena, Felipe Zamarripa, Maximilien Barret, Nicholas Eleftheriadis, Valerio Balassone, Haruhiro Inoue, Michel Kahaleh
BACKGROUND: Per-oral endoscopic myotomy (POEM) has become an accepted treatment for patients with achalasia. Despite its excellent efficacy rate of greater than 80%, a small percentage of patients remain symptomatic postprocedure. Limited data exist as to the best management for recurrence of symptoms after POEM. We present the first international, multicenter experience on the efficacy and safety of a repeat POEM in the management of achalasia. METHODS: Patients who underwent a redo POEM from 15 centers in 9 countries were included in a dedicated registry...
October 15, 2016: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/27733165/two-prophylactic-medication-approaches-in-addition-to-a-pain-control-regimen-for-early-medical-abortion%C3%A2-%C3%A2-63-days-gestation-with-mifepristone-and-misoprostol-study-protocol-for-a-randomized-controlled-trial
#9
Monica V Dragoman, Daniel Grossman, Nathalie Kapp, Nguyen My Huong, Ndema Habib, Duong Lan Dung, Anand Tamang
BACKGROUND: Pain is often cited as one of the worst features of medical abortion. Further, inadequate pain management may motivate some women to seek unnecessary clinical care. There is a need to identify effective methods for pain control in this setting. METHODS/DESIGN: We propose a randomized, placebo-controlled trial. 576 participants (288 nulliparous; 288 parous) from study sites in Nepal, South Africa and Vietnam will be randomly allocated to one of three treatments: (1) ibuprofen 400 mg PO and metoclopramide 10 mg PO; (2) tramadol 50 mg PO and a placebo; or (3) two placebo pills, to be taken immediately before misoprostol and repeated once four hours later...
October 12, 2016: Reproductive Health
https://www.readbyqxmd.com/read/27706771/detection-of-aneuploidies-in-spontaneous-abortions-by-quantitative-fluorescent-pcr-with-short-tandem-repeat-markers-a-retrospective-study
#10
F F Coelho, F K Marques, M S Gonçalves, V C O Almeida, E C C Mateo, A C S Ferreira
Approximately 10-15% of all pregnancies end in spontaneous abortions. Many factors can lead to embryonic loss; however, it has been well established that over 50% of all miscarriages result from chromosomal abnormalities, primarily aneuploidies (>96%). Identifying the cause of miscarriage can significantly reduce the psychological stress in women, and enable better genetic counseling for a future pregnancy. Quantitative fluorescent polymerase chain reaction (QF-PCR) has been previously used in the study of chromosomal abnormalities...
September 23, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27689407/contraceptive-counselling-of-women-seeking-abortion-a-qualitative-interview-study-of-health-professionals-experiences
#11
Helena Kilander, Birgitta Salomonsson, Johan Thor, Jan Brynhildsen, Siw Alehagen
OBJECTIVES: A substantial proportion of women who undergo an abortion continue afterwards without switching to more effective contraceptive use. Many subsequently have repeat unintended pregnancies. This study, therefore, aimed to identify and describe health professionalś experiences of providing contraceptive counselling to women seeking an abortion. METHODS: We interviewed 21 health professionals (HPs), involved in contraceptive counselling of women seeking abortion at three differently sized hospitals in Sweden...
September 30, 2016: European Journal of Contraception & Reproductive Health Care
https://www.readbyqxmd.com/read/27688719/improving-the-quality-of-postabortion-care-services-in-togo-increased-uptake-of-contraception
#12
Stembile Mugore, Ntapi Tchiguiri K Kassouta, Boniface Sebikali, Laurel Lundstrom, Abdulmumin Saad
High-quality postabortion care (PAC) services that include family planning counseling and a full range of contraceptives at point of treatment for abortion complications have great potential to break the cycle of repeat unintended pregnancies and demand for abortions. We describe the first application of a systematic approach to quality improvement of PAC services in a West African country. This approach-IntraHealth International's Optimizing Performance and Quality (OPQ) approach-was applied at 5 health care facilities in Togo starting in November 2014...
September 28, 2016: Global Health, Science and Practice
https://www.readbyqxmd.com/read/27678463/morphometric-comparison-by-the-isas-%C3%A2-casa-dnaf-system-of-two-techniques-for-the-evaluation-of-dna-fragmentation-in-human-spermatozoa
#13
Sara Sadeghi, Almudena García-Molina, Ferran Celma, Anthony Valverde, Sogol Fereidounfar, Carles Soler
DNA fragmentation has been shown to be one of the causes of male infertility, particularly related to repeated abortions, and different methods have been developed to analyze it. In the present study, two commercial kits based on the SCD technique (Halosperm ® and SDFA) were evaluated by the use of the DNA fragmentation module of the ISAS ® v1 CASA system. Seven semen samples from volunteers were analyzed. To compare the results between techniques, the Kruskal-Wallis test was used. Data were used for calculation of Principal Components (two PCs were obtained), and subsequent subpopulations were identified using the Halo, Halo/Core Ratio, and PC data...
November 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27664334/molecular-typing-of-isolates-obtained-from-aborted-foetuses-in-brucella-free-holstein-dairy-cattle-herd-after-immunisation-with-brucella-abortus-rb51-vaccine-in-egypt
#14
Gamal Wareth, Falk Melzer, Denny Böttcher, Mohamed El-Diasty, Mohamed El-Beskawy, Nesma Rasheed, Gernot Schmoock, Uwe Roesler, Lisa D Sprague, Heinrich Neubauer
Bovine brucellosis is endemic in Egypt in spite of application of surveillance and control measures. An increase of abortions was reported in a Holstein dairy cattle herd with 600 animals in Damietta governorate in Egypt after immunisation with Brucella (B.) abortus RB51 vaccine. Twenty one (10.6%) of 197 vaccinated cows aborted after 3 months. All aborted cows had been tested seronegative for brucellosis in the past 3 years. B. abortus was isolated from four foetuses. Conventional biochemical and bacteriological identification and polymerase chain reaction (PCR) confirmed two B...
September 21, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27664217/immediate-versus-delayed-insertion-of-an-etonogestrel-releasing-implant-at-medical-abortion-a-randomized-controlled-equivalence-trial
#15
Helena Hognert, Helena Kopp Kallner, Sharon Cameron, Christina Nyrelli, Izabella Jawad, Rebecca Heller, Annette Aronsson, Ingela Lindh, Lina Benson, Kristina Gemzell-Danielsson
STUDY QUESTION: Does a progestin releasing subdermal contraceptive implant affect the efficacy of medical abortion if inserted at the same visit as the progesterone receptor modulator, mifepristone, at medical abortion? SUMMARY ANSWER: A etonogestrel releasing subdermal implant inserted on the day of mifepristone did not impair the efficacy of the medical abortion compared with routine insertion at 2-4 weeks after the abortion. WHAT IS ALREADY KNOWN: The etonogestrel releasing subdermal implant is one of the most effective long acting reversible contraceptive methods...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27621046/serum-human-chorionic-gonadotropin-hcg-trend-within-the-first-few-days-after-medical-abortion-a-prospective-study
#16
Katherine D Pocius, Deborah Bartz, Rie Maurer, Asha Stenquist, Jennifer Fortin, Alisa B Goldberg
OBJECTIVES: To prospectively describe the decline in serum human chorionic gonadotropin (hCG) in the first 5 days after complete medical abortion and evaluate the influence of initial hCG and gestational duration. STUDY DESIGN: We conducted a prospective, physiologic study of women ≤63 days gestation who underwent medical abortion with 200 mg mifepristone and 800 mcg buccal misoprostol. We stratified enrollment into two gestational cohorts, <49 days and 49-63 days, to ensure gestational variability...
September 13, 2016: Contraception
https://www.readbyqxmd.com/read/27614789/intralipid-supplementation-in-women-with-recurrent-spontaneous-abortion-and-elevated-levels-of-natural-killer-cells
#17
Dina M R Dakhly, Yomna A Bayoumi, Mohamed Sharkawy, Sherine H Gad Allah, Mohamed A Hassan, Hisham M Gouda, Ahmed T Hashem, Dina L Hatem, Mona F Ahmed, Waleed El-Khayat
OBJECTIVE: To investigate the efficacy of intralipid supplementation in women with recurrent spontaneous abortion (RSA) and elevated natural killer cell activity undergoing in vitro fertilization/intracytoplasmic sperm injection. METHODS: Between February 10, 2013, and April 30, 2015, a double-blind randomized controlled study was conducted at a center in Egypt. Women with unexplained secondary infertility, RSA, and elevated levels of natural killer cells (>12%) were enrolled and randomly assigned to receive intralipid (2mL diluted at 20% in 250mL saline) or saline (250mL) infusion on the day of oocyte retrieval using random numbers and sealed envelopes...
December 2016: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/27607859/effects-of-depot-medroxyprogesterone-acetate-injection-timing-on-medical-abortion-efficacy-and-repeat-pregnancy-a-randomized-controlled-trial
#18
Elizabeth G Raymond, Mark A Weaver, Karmen S Louie, Yi-Ling Tan, Manuel Bousiéguez, Ana Gabriela Aranguré-Peraza, Elba M Lugo-Hernández, Patricio Sanhueza, Alisa B Goldberg, Kelly R Culwell, Clair Kaplan, Lisa Memmel, Sarita Sonalkar, Roxanne Jamshidi, Beverly Winikoff
OBJECTIVE: To evaluate the effects of timing of depot medroxyprogesterone acetate injection on medical abortion outcome and risk of repeat pregnancy within the subsequent 6 months. METHODS: In a multinational randomized trial, we assigned women undergoing medical abortion who wanted depot medroxyprogesterone acetate to administration either with mifepristone (Quickstart group) or after the abortion (Afterstart group). We ascertained abortion outcome, pregnancies, and contraception use over 7 months...
October 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27583448/clinical-outcomes-and-women-s-experiences-before-and-after-the-introduction-of-mifepristone-into-second-trimester-medical-abortion-services-in-south-africa
#19
Deborah Constant, Jane Harries, Thokozile Malaba, Landon Myer, Malika Patel, Gregory Petro, Daniel Grossman
OBJECTIVE: To document clinical outcomes and women's experiences following the introduction of mifepristone into South African public sector second-trimester medical abortion services, and compare with historic cohorts receiving misoprostol-only. METHODS: Repeated cross-sectional observational studies documented service delivery and experiences of women undergoing second-trimester medical abortion in public sector hospitals in the Western Cape, South Africa. Women recruited to the study in 2008 (n = 84) and 2010 (n = 58) received misoprostol only...
2016: PloS One
https://www.readbyqxmd.com/read/27582740/bacteria-vs-bacteriophages-parallel-evolution-of-immune-arsenals
#20
REVIEW
Muhammad A B Shabbir, Haihong Hao, Muhammad Z Shabbir, Qin Wu, Adeel Sattar, Zonghui Yuan
Bacteriophages are the most common entities on earth and represent a constant challenge to bacterial populations. To fend off bacteriophage infection, bacteria evolved immune systems to avert phage adsorption and block invader DNA entry. They developed restriction-modification systems and mechanisms to abort infection and interfere with virion assembly, as well as newly recognized clustered regularly interspaced short palindromic repeats (CRISPR). In response to bacterial immune systems, bacteriophages synchronously evolved resistance mechanisms, such as the anti-CRISPR systems to counterattack bacterial CRISPR-cas systems, in a continuing evolutionary arms race between virus and host...
2016: Frontiers in Microbiology
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