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Leah D McGurn, Maryam Moazami-Goudarzi, Sean A White, Tannu Suwal, Beant Brar, Jason Q Tang, George S Espie, Matthew S Kimber
CcaA is a b-carbonic anhydrase that is a component of the carboxysomes of a subset of b-cyanobacteria. This protein, which has a characteristic C-terminal extension of unknown function, is recruited to the carboxysome via interactions with CcmM, which is itself a γ-carbonic anhydrase homolog with enzymatic activity in many, but not all cyanobacteria. We have determined the structure of CcaA from Synechocystis sp. PCC 6803 at 1.45 Å. In contrast to the dimer of dimers organization of most bacterial β-carbonic anhydrases, or the loose dimer-of-dimers-of-dimers organization found in the plant enzymes, CcaA shows a well-packed trimer-of-dimers organization...
October 11, 2016: Biochemical Journal
J Parker, Y Oh, Y Moazami, J G Pierce, P L Loziuk, R A Dean, D C Muddiman
Ubiquitination is a dynamic process that is responsible for regulation of cellular responses to stimuli in a number of biological systems. Previous efforts to study this post-translational modification have focused on protein enrichment; however, recent research utilizes the presence of the di-glycine (Gly-Gly) remnants following trypsin digestion to immuno-enrich ubiquitinated peptides. Monoclonal antibodies developed to the cleaved ubiquitin modification epitope, (tert-butoxycarbonyl) glycylglycine (Boc-Gly-Gly-NHS)(1), are used to identify the Gly-Gly signature...
November 1, 2016: Analytical Biochemistry
Sangjin Lee, Jason N Katz, Ulrich P Jorde, Nader Moazami, Ranjit John, Kartik S Sundareswaran, David J Farrar, O H Frazier
There is insufficient data on patients with small body size to determine if this should be considered a risk factor for continuous-flow left ventricular assist device (CF-LVAD) support. We sought to evaluate survival outcomes, adverse events, and functional status of CF-LVAD patients with body surface area (BSA)<1.5 m in a large national registry. Adults with BSA<1.5 m (n=128) implanted with a HeartMate II (HMII)-LVAD from the INTERMACs registry from April 2008 to December 2012 formed this cohort. Outcomes were compared to HMII Bridge to Transplant (BTT) and Destination Therapy (DT) Post-Approval Studies...
August 22, 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Jamil Mohammadi, Saied Saeed Hosseiny Davarani, Hamid Reza Moazami
Electromembrane extraction (EME) was carried out using a novel instrumentation capable of impedometric monitoring of the system during the extraction. This instrumentation involves a classical two-electrode assembly fed by two time-resolved potential functions, the first for the extraction of analyte and the second for obtaining the impedance information. The impedometric analysis of the system was achieved by Laplace transformation of the current recorded during the extraction. It has been shown that the obtained impedance information can be converted to very useful knowledge about time dependence of double layer capacitance, kinetics of analyte depletion, total permeability of the SLM and the effect of experimental parameters on system behavior...
August 31, 2016: Analytica Chimica Acta
Mathieu Gautier, Katayoun Moazami-Goudarzi, Hubert Levéziel, Hugues Parinello, Cécile Grohs, Stéphanie Rialle, Rafał Kowalczyk, Laurence Flori
As the largest European herbivore, the wisent (Bison bonasus) is emblematic of the continent wildlife but has unclear origins. Here, we infer its demographic and adaptive histories from two individual whole-genome sequences via a detailed comparative analysis with bovine genomes. We estimate that the wisent and bovine species diverged from 1.7 × 10(6) to 850,000 years before present (YBP) through a speciation process involving an extended period of limited gene flow. Our data further support the occurrence of more recent secondary contacts, posterior to the Bos taurus and Bos indicus divergence (∼150,000 YBP), between the wisent and (European) taurine cattle lineages...
November 2016: Molecular Biology and Evolution
David Horvath, Nicole Byram, Jamshid H Karimov, Barry Kuban, Gengo Sunagawa, Leonard A R Golding, Nader Moazami, Kiyotaka Fukamachi
Cleveland Clinic's continuous-flow total artificial heart (CFTAH) provides systemic and pulmonary circulations using one assembly (one motor, two impellers). The right pump hydraulic output to the pulmonary circulation is self-regulated by the rotating assembly's passive axial movement in response to atrial differential pressure to balance itself to the left pump output. This combination of features integrates a biocompatible, pressure-balancing regulator with a double-ended pump. The CFTAH requires no flow or pressure sensors...
July 12, 2016: Artificial Organs
Shabnam Shamriz, Hamideh Ofoghi, Nasrin Moazami
BACKGROUND: Recombinant protein technology has revolutionized the world of biology and medicine. Following this progress, fusion protein technology, as a novel innovation, has opened new horizons for the development of proteins that do not naturally exist. Fusion proteins are generated via genetically fusing two or more genes coding for separate proteins, thus the product is a single protein having functional properties of both proteins. As an indispensable element in fusion protein construction, linkers are used to separate the functional domains in order to improve their expression, folding and stability...
September 1, 2016: Computers in Biology and Medicine
Mozhagan Moazami-Goudarzi, Majid Farshdousti-Hagh, Abbasali Hoseinpour-Feizi, Mehdi Talebi, Ali Akbar Movassaghpour-Akbari, Karim Shams-Asanjan, Jamal Eyvazi-Ziyaee, Morteza Seifi
BACKGROUND: BCL-2 is the most important anti-apoptotic regulator and Bax is a pro-apoptotic protein. The status of these parameters or the ration of BCL-2 to BAX is important in malignant cell fate as well as normal cells. METHODS: Sixty-two ALL patients and 62 healthy sex-and age-matched controls were studied. After genotyping, the promoter region of the BAX and BCL-2 genes by RFLP-PCR method the patients were classified in nine prognostic groups, after that, the overall survival ratio of each score was compared with others pair-wise or between groups...
2016: Caspian Journal of Internal Medicine
Joshua M Stern, Saman Moazami, Yunping Qiu, Irwin Kurland, Zigui Chen, Ilir Agalliu, Robert Burk, Kelvin P Davies
The trillions of microbes that colonize our adult intestine are referred to as the gut microbiome (GMB). Functionally it behaves as a metabolic organ that communicates with, and complements, our own human metabolic apparatus. While the relationship between the GMB and kidney stone disease (KSD) has not been investigated, dysbiosis of the GMB has been associated with diabetes, obesity and cardiovascular disease. In this pilot study we sought to identify unique changes in the GMB of kidney stone patients compared to patients without KSD...
October 2016: Urolithiasis
Jamshid H Karimov, Gengo Sunagawa, David Horvath, Kiyotaka Fukamachi, Randall C Starling, Nader Moazami
Failure of the right ventricle represents a significant clinical problem and may have different causes, with rates varying between 5% and 50% in patients supported by a left ventricular assist device (LVAD). However, treatment options and device development for right ventricular failure (RVF) have significantly lagged behind those for LVADs. Newer technologies designed or adapted for RV support are needed to provide adequate long-term circulatory support. In this review, we discuss (1) the significance of RVF and its physiologic implications, (2) device constraints affecting treatment options for RVF, and (3) implantable VADs potentially available for RV support...
August 2016: Annals of Thoracic Surgery
Aurélie Allais-Bonnet, Johan Castille, Maëlle Pannetier, Bruno Passet, Maëva Elzaïat, Marjolaine André, Fatemeh Montazer-Torbati, Katayoun Moazami-Goudarzi, Jean-Luc Vilotte, Eric Pailhoux
Three genes of the prion protein gene family are expressed in gonads. Comparative analyses of their expression patterns in mice and goats revealed constant expression of PRNP and SPRN in both species and in both male and female gonads, but with a weaker expression of SPRN. By contrast, expression of PRND was found to be sex-dimorphic, in agreement with its role in spermatogenesis. More importantly, our study revealed that PRND seems to be a key marker of foetal Leydig cells specifically in goats, suggesting a yet unknown role for its encoded protein Doppel during gonadal differentiation in nonrodent mammals...
January 2016: FEBS Open Bio
Robert J Steffen, Kyle G Miletic, Dean P Schraufnagel, Patrick R Vargo, Kiyotaka Fukamachi, Robert D Stewart, Nader Moazami
End-stage heart failure affects thousands of children yearly and mechanical circulatory support is used at many points in their care. Extracorporeal membrane oxygenation supports both the failing heart and lungs, which has led to its use as an adjunct to cardiopulmonary resuscitation as well as in post-operative cardiogenic shock. Continuous-flow ventricular assist devices (VAD) have replaced pulsatile-flow devices in adults and early studies have shown promising results in children. The Berlin paracorporeal pulsatile VAD recently gained U...
May 2016: Expert Review of Medical Devices
Omid Amir-Moazami, Yalin Emre
No abstract text is available yet for this article.
February 2016: Médecine Sciences: M/S
Amanda R Vest, Stanley M Mistak, Rory Hachamovitch, Maria M Mountis, Nader Moazami, James B Young
BACKGROUND: Diabetes mellitus (DM) is a risk factor for mortality among patients with heart failure as well as for patients who undergo cardiothoracic surgery. However it is unknown whether DM is associated with increased mortality or major complications during continuous-flow left ventricular assist device (CF-LVAD) support. METHODS AND RESULTS: We retrospectively reviewed 300 consecutive adults who received CF-LVADs at a single center in the years 2006-2013; 129 patients had DM before LVAD, as defined by American Diabetes Association criteria (HbA1c ≥6...
October 2016: Journal of Cardiac Failure
Gengo Sunagawa, Nicole Byram, Jamshid H Karimov, David J Horvath, Nader Moazami, Randall C Starling, Kiyotaka Fukamachi
BACKGROUND: We recently reported using bench testing that the Thoratec HeartMate II at 6,000 rpm contributed to hemodynamics when the heart had not recovered well, making weaning assessment questionable. In this bench study, we characterized hemodynamics and pump flow of the HeartWare HVAD at 1,800 rpm, the lowest speed commonly used to assess clinical recovery. METHODS: The HVAD was operated in a mock loop at 1,800, 2,400, and 3,000 rpm. We acquired pressure-flow curves in each steady state...
June 2016: Annals of Thoracic Surgery
Gengo Sunagawa, David J Horvath, Jamshid H Karimov, Nader Moazami, Kiyotaka Fukamachi
A total artificial heart (TAH) is the sole remaining option for patients with biventricular failure who cannot be rescued by left ventricular assist devices (LVADs) alone. However, the pulsatile TAH in clinical use today has limitations: large pump size, unknown durability, required complex anticoagulation regimen, and association with significant postsurgical complications. That pump is noisy; its large pneumatic driving lines traverse the body, with bulky external components for its drivers. Continuous-flow pumps, which caused a paradigm shift in the LVAD field, have already contributed to the rapidly evolving development of TAHs...
2016: Expert Review of Medical Devices
Alexander Kantorovich, Jodie M Fink, Michael A Militello, Seth R Bauer, Edward G Soltesz, Nader Moazami
Appropriate early anticoagulation after left ventricular assist device (LVAD) implantation has not been established with practices ranging from no anticoagulation to early heparinization. The goal of this study was to evaluate the efficacy and morbidity of three strategies before initiating oral anticoagulation therapy. This was a noninterventional, retrospective, matched historical control cohort study. The primary and secondary endpoints were thrombotic complications (TCs) and bleeding up to 30 days post-LVAD implantation...
March 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Jamshid H Karimov, Nader Moazami, Gengo Sunagawa, Mariko Kobayashi, Nicole Byram, Shiva Sale, Kimberly A Such, David J Horvath, Leonard A R Golding, Kiyotaka Fukamachi
The choice of optimal operative access technique for mechanical circulatory support device implantation ensures successful postoperative outcomes. In this study, we retrospectively evaluated the median sternotomy and lateral thoracotomy incisions for placement of the Cleveland Clinic continuous-flow total artificial heart (CFTAH) in a bovine model. The CFTAH was implanted in 17 calves (Jersey calves; weight range, 77.0-93.9 kg) through a median sternotomy (n = 9) or right thoracotomy (n = 8) for elective chronic implantation periods of 14, 30, or 90 days...
December 18, 2015: Artificial Organs
Nicholas G Smedira, Eugene H Blackstone, John Ehrlinger, Lucy Thuita, Christopher D Pierce, Nader Moazami, Randall C Starling
BACKGROUND: Data from 3 institutions revealed an abrupt increase in HeartMate II (Thoratec) pump thrombosis starting in 2011, associated with 48% mortality at 6 months without transplantation or pump exchange. We sought to discover if the increase occurred nationwide in Interagency Registry for Mechanically Assisted Circulatory Support (INTERMACS) data, and if so (1) determine if accelerated risk continued, (2) identify predictors, (3) investigate institutional variability, and (4) assess mortality after pump thrombosis...
December 2015: Journal of Heart and Lung Transplantation
Manal Khalifé, Fabienne Reine, Sophie Paquet-Fifield, Johan Castille, Laetitia Herzog, Marthe Vilotte, Mohammed Moudjou, Katayoun Moazami-Goudarzi, Samira Makhzami, Bruno Passet, Olivier Andréoletti, Didier Vilette, Hubert Laude, Vincent Béringue, Jean-Luc Vilotte
UNLABELLED: Mammalian prions are proteinaceous infectious agents composed of misfolded assemblies of the host-encoded, cellular prion protein (PrP). Physiologically, the N-terminal polybasic region of residues 23 to 31 of PrP has been shown to be involved in its endocytic trafficking and interactions with glycosaminoglycans or putative ectodomains of membrane-associated proteins. Several recent reports also describe this PrP region as important for the toxicity of mutant prion proteins and the efficiency of prion propagation, both in vitro and in vivo...
February 2016: Journal of Virology
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