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Aedes cry11

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https://www.readbyqxmd.com/read/24187584/investigating-the-genetics-of-bti-resistance-using-mrna-tag-sequencing-application-on-laboratory-strains-and-natural-populations-of-the-dengue-vector-aedes-aegypti
#1
Margot Paris, Sebastien Marcombe, Eric Coissac, Vincent Corbel, Jean-Philippe David, Laurence Després
Mosquito control is often the main method used to reduce mosquito-transmitted diseases. In order to investigate the genetic basis of resistance to the bio-insecticide Bacillus thuringiensis subsp. israelensis (Bti), we used information on polymorphism obtained from cDNA tag sequences from pooled larvae of laboratory Bti-resistant and susceptible Aedes aegypti mosquito strains to identify and analyse 1520 single nucleotide polymorphisms (SNPs). Of the 372 SNPs tested, 99.2% were validated using DNA Illumina GoldenGate® array, with a strong correlation between the allelic frequencies inferred from the pooled and individual data (r = 0...
November 2013: Evolutionary Applications
https://www.readbyqxmd.com/read/22178674/characterization-of-bacillus-thuringiensis-isolates-with-potential-for-control-of-aedes-aegypti-linnaeus-1762-diptera-culicidae
#2
F P Santos, J Lopes, G T Vilas-Bôas, J A C Zequi
Aedes aegypti (Linnaeus) is the vector of dengue virus in Brazil. Bioinsecticides based on Bacillus thuringiensis have shown satisfactory results in the control of Diptera, due to the production of proteins called Cry and Cyt. The aim of the present study was to select B. thuringiensis isolates carrying the cry and cyt genes, which are efficient in the control of Ae. aegypti. A collection of 27 isolates of B. thuringiensis, derived from various regions in Brazil, was evaluated using selective bioassays against A...
April 2012: Acta Tropica
https://www.readbyqxmd.com/read/15057351/in-vivo-binding-of-the-cry11bb-toxin-of-bacillus-thuringiensis-subsp-medellin-to-the-midgut-of-mosquito-larvae-diptera-culicidae
#3
Lina María Ruiz, César Segura, Judith Trujillo, Sergio Orduz
Bacillus thuringiensis subsp. medellin produces numerous proteins among which 94 kDa known as Cry11Bb, has mosquitocidal activity. The mode of action of the Cry11 proteins has been described as similar to those of the Cry1 toxins, nevertheless, the mechanism of action is still not clear. In this study we investigated the in vivo binding of the Cry11Bb toxin to the midgut of the insect species Anopheles albimanus, Aedes aegypti, and Culex quinquefasciatus by immunohistochemical analysis. Spodoptera frugiperda was included as negative control...
February 2004: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/12957913/diversity-of-bacillus-thuringiensis-strains-from-latin-america-with-insecticidal-activity-against-different-mosquito-species
#4
COMPARATIVE STUDY
Jorge E Ibarra, M Cristina del Rincón, Sergio Ordúz, David Noriega, Graciela Benintende, Rose Monnerat, Leda Regis, Cláudia M F de Oliveira, Humberto Lanz, Mario H Rodriguez, Jorge Sánchez, Guadalupe Peña, Alejandra Bravo
The characterization of selected Bacillus thuringiensis strains isolated from different Latin America countries is presented. Characterization was based on their insecticidal activity against Aedes aegypti, Culex quinquefasciatus, and Anopheles albimanus larvae, scanning electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and plasmid profiles as well as PCR analysis using novel general and specific primers for cry and cyt genes encoding proteins active against mosquitoes (cyt1, cyt2, cry2, cry4A, cry4B, cry10, cry11, cry17, cry19, cry24, cry25, cry27, cry29, cry30, cry32, cry39, and cry40)...
September 2003: Applied and Environmental Microbiology
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