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Vibrio parahaemolyticus

Zhong-Guo Shan, Ke-Xin Zhu, Fang-Yi Chen, Jie Liu, Bei Chen, Kun Qiao, Hui Peng, Ke-Jian Wang
A new gene homologous to the reported antimicrobial peptide (AMP) hyastatin from Hyas araneus was screened in the SSH library constructed from the hemocytes of Scylla paramamosain, and named SpHyastatin. In vivo study showed that SpHyastatin was predominantly expressed in hemocytes of S. paramamosain. With the challenge of either Vibrio parahaemolyticus or lipopolysaccharide (LPS), SpHyastatin showed a positive response, meaning that it was probably involved in the immune reaction against bacterial infection in vivo...
October 14, 2016: Fish & Shellfish Immunology
Xinfeng Liu, Yuyao Guan, Shiliang Cheng, Yidan Huang, Qin Yan, Jun Zhang, Guanjun Huang, Jian Zheng, Tianqiang Liu
Vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. It is also a foodborne pathogen that causes diarrhea in humans. The aim of this study is to develop an immunochromatographic lateral flow assay (LFA) for rapid detection of V. parahaemolyticus in both aquatic products and human feces of diarrheal patients. Two monoclonal antibody (MAb) pairs, GA1a-IC9 and IC9-KB4c, were developed and proven to be highly specific and sensitive to V. parahaemolyticus...
October 14, 2016: Journal of Microbiological Methods
H W Kim, Y J Hong, J I Jo, S D Ha, S H Kim, H J Lee, M S Rhee
Microbiological quality of 206 raw ready-to-eat seafood samples were investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster, and squid) and distribution channels (fishery, hyper, and online market). Enumeration of Aerobic Plate Count (APC) and total coliforms (TC) and pathogenic bacteria (Bacillus cereus, Staphylococcus aureus, and Vibrio parahaemolyticus) were performed, and level of microbiological quality were classified into four groups: satisfactory, acceptable, unsatisfactory, and unacceptable...
October 16, 2016: Letters in Applied Microbiology
Ying Huang, Wen Wang, Qian Ren
C1q is the target recognition sequence of the classical complement pathway and a major link that connects innate and acquired immunity. In this study, a C1qDC homolog, HcC1qDC5, from the triangle-shell pearl mussel (Hyriopsis cumingii) was identified. The complete nucleotide sequence of HcC1qDC5 cDNA consists of a 5'-untranslated terminal region (UTR) of 123 bp, a 3'-UTR of 105 bp with a poly(A) tail, and an open reading frame (ORF) of 1344 bp, which encodes a polypeptide of 447 amino acids. HcC1qDC5 contains a signal peptide and three typical C1q domains...
October 7, 2016: Fish & Shellfish Immunology
Guadalupe Barrera-Escorcia, Irma Wong-Chang, Carlos Leopoldo Fernández-Rendón, Alfonso Vázquez Botello, Bruno Gómez-Gil, Marcial Leonardo Lizárraga-Partida
Oysters can accumulate potentially pathogenic water bacteria. The objective of this study was to compare two procedures to quantify Vibrio species present in oysters to determine the most sensitive method. We analyzed oyster samples from the Gulf of Mexico, commercialized in Mexico City. The samples were inoculated in tubes with alkaline peptone water (APW), based on three tubes and four dilutions (10(-1) to 10(-4)). From these tubes, the first quantification of Vibrio species was performed (most probable number (MPN) from tubes) and bacteria were inoculated by streaking on thiosulfate-citrate-bile salts-sucrose (TCBS) petri dishes...
November 2016: Environmental Monitoring and Assessment
Vengadesh Letchumanan, Hooi-Leng Ser, Kok-Gan Chan, Bey-Hing Goh, Learn-Han Lee
No abstract text is available yet for this article.
2016: Frontiers in Microbiology
P Li, X F Jiang, W B Guo, J Yan, K Y Zhou
Two heat-shock protein (HSP) 70 family transcripts, heat-shock protein 70 cognate 5 and heat-shock protein 70 cognate 3 (designated as EsHSC70-5 and EsHSC70-3, respectively), were isolated from the Chinese mitten crab Eriocheir sinensis and their expression profiles were evaluated for their responsiveness to larval development and immune challenge in adult crabs. The HSPs exhibited 45-89% identity with other heat-shock proteins, and they shared similar structural features. EsHSC70 mRNA expression was detected not only during infection but also during the developmental larval stages...
September 16, 2016: Genetics and Molecular Research: GMR
Hui Li, Xiao Chu, Bo Peng, Xuan-Xian Peng
Molecular breeding via DNA shuffling directs the evolution of vaccines with desired traits. In the present study, polyvalent OmpA vaccines were generated by DNA shuffling of five ompA genes from four species of bacteria Vibrio parahaemolyticus, V. alginolyticus, Edwardsiella tarda and Escherichia coli. First, a new hybrid OmpA was constructed using VA0764 primers and used for construction of a prokaryotic expressing library PompAs-FV containing 84 ompAs, which were validated by PCR and SDS/PAGE. Then, the 84 ompAs were used to construct a eukaryotic expressing library EompAs-FV for preparing DNA vaccines...
September 30, 2016: Fish & Shellfish Immunology
Nattan Stalin, Pappu Srinivasan
Phage therapy is an alternative and eco-friendly biocontrol agent to prevent and control multidrug resistant bacteria in the aquatic system. The aim of this study is to isolate and characterize the Vibrio parahaemolyticus and its potential lytic phage from Penaeus monodon growing-out by rearing in shrimp ponds in Palk Strait, South East coast of India. The conventional phenotypic characteristics and molecular identification was confirmed using 16S rRNA sequence and to determine the antibiotic resistant profiles...
September 30, 2016: Biologicals: Journal of the International Association of Biological Standardization
Kewen Cheng, Daodong Pan, Jun Teng, Li Yao, Yingwang Ye, Feng Xue, Fan Xia, Wei Chen
Rapid detection of pathogens is of great significance for food safety and disease diagnosis. A new colorimetric method for rapid and easy detection of Vibrio parahaemolyticus (V. parahaemolyticus or Vp) has been developed in this research. A specific sequence was designed and integrated with the forward primer for molecular detection of Vp. This specific sequence was tested and treated as the horseradish peroxidase (HRP)-mimicking DNAzyme and could be amplified during the polymerase chain reaction (PCR) process...
2016: Sensors
Ruichao Li, Lianwei Ye, Zhiwei Zheng, Edward Wai Chi Chan, Sheng Chen
This study reports the first detection of blaVEB-2 gene in Vibrio parahaemolyticus strain isolated from a shrimp sample. The blaVEB-2 was carried on a novel Inc type plasmid, was likely to originate from aquatic organisms upon comparison with other known genetic elements in the GenBank. However, the plasmid contains resistance elements usually harbored by members of Enterobacteriaceae, suggesting that gene transfer events occurred and contributed to the formation of this multidrug resistance-encoding plasmid...
September 19, 2016: Antimicrobial Agents and Chemotherapy
Kajal Chakraborty, Bini Thilakan, Rekha Devi Chakraborty, Vamshi Krishna Raola, Minju Joy
The brown seaweed, Sargassum myriocystum associated with heterotrophic bacterium, Bacillus subtilis MTCC 10407 (JF834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria Aeromonas hydrophila, Vibrio vulnificus, and Vibrio parahaemolyticus. B. subtilis MTCC 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. Using bioassay-guided fractionation, two new antibacterial O-heterocyclic compounds belonging to pyranyl benzoate analogs of polyketide origin, with activity against pathogenic bacteria, have been isolated from the ethyl acetate extract of B...
September 13, 2016: Applied Microbiology and Biotechnology
Xingxing Dong, Zhi Li, Xiaohong Wang, Min Zhou, Li Lin, Yang Zhou, Jinquan Li
Vibrio parahaemolyticus usually occurs in coastal areas and is generally recognized as a marine bacterium. It has become the leading cause of gastroenteritis worldwide. In the present study, 96 V. parahaemolyticus isolates were obtained from freshwater crayfish (Procambarus clarkii) and classified by multilocus sequence typing. Fifty-three sequence types (STs) were identified among the 96 isolates analyzed, 38 of which were novel STs. These isolates fell into six groups and 42 singletons, suggesting a high level of genetic diversity...
September 8, 2016: International Journal of Food Microbiology
Xin Huang, Jin-Ling Feng, Min Jin, Qian Ren, Wen Wang
C-type lectins (CTLs) play important roles in the innate immunity of invertebrates. In this study, a novel CTL with a single carbohydrate recognition domain (CRD) containing an EPN (Glu-Pro-Asn) motif was identified from the giant freshwater prawn Macrobrachium rosenbergii. This CTL was designated as MrCTL. The cDNA of MrCTL is 1788 bp with a 657 bp open reading frame that encodes a protein of 218 amino acids. The cDNA and genome sequences of MrCTL show a polymorphism that leads to MrCTL isoforms. MrCTL was highly expressed in the gills and intestine of normal prawn, and its transcription increased after Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge...
September 9, 2016: Fish & Shellfish Immunology
Xin Lu, Haijian Zhou, Xiaoli Du, Sha Liu, Jialiang Xu, Zhigang Cui, Bo Pang, Biao Kan
Vibrio parahaemolyticus is a common seafood-borne pathogenic bacterium which causes gastroenteritis in humans. Continuous surveillance on the molecular characters of the clinical and environmental V. parahaemolyticus strains needs to be conducted for the epidemiological and genetic purposes. To generate a picture of the population distribution of V. parahaemolyticus in eastern China isolated from clinical cases of gastroenteritis and environmental samples, we investigated the genetic and evolutionary relationships of the strains using the commonly used multi-locus sequence typing (MLST, in which seven house-keeping genes are used in the protocol)...
September 5, 2016: Infection, Genetics and Evolution
T J Cho, N H Kim, S A Kim, J H Song, M S Rhee
Knowing the survival characteristics of foodborne pathogens in raw ready-to-eat (RTE) seafood is the key to predicting whether they pose a microbiological hazard. The present study examined the survival of Escherichia coli O157:H7, Salmonella Typhimurium, Vibrio parahaemoliticus, Listeria monocytogenes, and Staphylococcus aureus in raw RTE crab marinated in soy sauce. Inoculated crabs (initial bacterial population=4.1-4.4logCFU/g) were immersed in soy sauce and then stored at refrigeration (5°C) or room temperature (22°C) for up to 28days...
August 30, 2016: International Journal of Food Microbiology
Leda Restrepo, Bonny Bayot, Irma Betancourt, Andres Pinzón
Vibrio parahaemolyticus is a pathogenic bacteria which has been associated to the early mortality syndrome (EMS) also known as hepatopancreatic necrosis disease (AHPND) causing high mortality in shrimp farms. Pathogenic strains contain two homologous genes related to insecticidal toxin genes, PirA and PirB, these toxin genes are located on a plasmid contained within the bacteria. Genomic sequences have allowed the finding of two strains with a divergent structure related to the geographic region from where they were found...
September 2016: Genomics Data
Sasiwipa Tinwongger, Yuki Nochiri, Jumroensri Thawonsuwan, Reiko Nozaki, Hidehiro Kondo, Sharda Prasad Awasthi, Atsushi Hinenoya, Shinji Yamasaki, Ikuo Hirono
AIMS: To investigate the virulence of the Vp_PirAB-like genes in Vibrio parahaemolyticus-AHPND causing strain and factors that associate with the virulence level. METHODS AND RESULTS: The virulence of Vp_PirAB-like were examined using a non-virulent strain FP11 of V. parahaemolyticus transformed with a plasmid harboring Vp_PirAB-like genes and then used it to challenge shrimp Litopenaeus vannamei and Marsupenaeus japonicus. Both species experienced 100% mortality 10 days post-infection...
August 13, 2016: Journal of Applied Microbiology
Hui Yang, Shihao Li, Fuhua Li, Kuijie Yu, Fusheng Yang, Jianhai Xiang
Anti-lipopolysaccharide factors (ALFs) with a LPS-binding domain (LBD) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. FcALF2 was one isoform of ALFs isolated from the Chinese shrimp Fenneropenaeus chinensis. Our previous study showed that a modified LBD domain (named LBDv) of FcALF2 exhibited a highly enhanced antimicrobial activity. In the present study, a modified FcALF2 gene (mFcALF2), in which the LBD was substituted by LBDv, was designed and synthesized...
2016: Marine Drugs
D Isui Aguilar-Salvador, Guy Tran Van Nhieu
Bacterial type three secretion systems (T3SSs) are central to virulence, but the processes stimulating their activation are unclear. In this issue of Cell Host & Microbe, Blondel et al. (2016) perform a genome-wide screen using CRISPR/Cas technology to identify a role for cell surface sulfation and fucosylation in cytotoxicity mediated by Vibrio parahaemolyticus T3SSs.
August 10, 2016: Cell Host & Microbe
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