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Vibrio parahaemolyticus

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https://www.readbyqxmd.com/read/28221954/molecular-detection-of-the-three-major-pathogenic-vibrio-species-from-seafood-products-and-sediments-in-tunisia-using-real-time-pcr
#1
Morsi Gdoura, Hanen Sellami, Hanen Nasfi, Rahma Trabelsi, Sabeur Mansour, Touraya Attia, Siwar Nsaibia, Tatiana Vallaeys, Radhouane Gdoura, Mariam Siala
: Vibrio spp. have emerged as a serious threat to human health worldwide. V. parahaemolyticus , V. cholerae , and V. vulnificus pose a considerable public health risk in Tunisia because they cause sporadic and epidemic foodborne infections associated with the consumption of raw or undercooked contaminated seafood. More recently, toxR-positive V. alginolyticus was also reported to be a potential source of contaminated seafood. A total of 247 samples, including 113 fishes ( Labrus viridis , Penaeus kerathurus , Diplodus annularis , Diplodus sparaillon , Scorparna porcus , Sarpa salpa , Dentex dentex , Scorparna scrofa , Sardinella aurita , Trachurus trachurus , Synodus saurus , Pagellus erythrinus , and Metapenaeus monoceros ), 83 clams ( Ruditapes decussatus species), 30 seawater samples, and 21 sediment samples were analyzed using traditional culture methods (ISO/TS 21872-1; International Organization for Standardization 2007) and a conventional PCR method for Vibrio spp...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28214599/hctoll3-was-involved-in-anti-vibrio-defense-in-freshwater-pearl-mussel-hyriopsis-cumingii
#2
Hong-Wei Zhang, Ying Huang, Xin Man, Yue Wang, Kai-Min Hui, Shao-Wu Yin, Xiao-Wen Zhang
Toll-like receptors (TLRs) play an important role in the activation of innate immune response but their functions in bivalves remain largely unknown. In this study, we identified a TLR from the freshwater pearl mussel Hyriopsis cumingii (HcToll3) and investigated its functions in immunity. The full-length cDNA of HcToll3 is 3852 bp and includes an open reading frame (ORF) of 3228 bp that encodes a polypeptide of 1075 amino acids. The predicted HcToll3 protein shares similar structural characteristics with other known Toll family proteins...
February 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28211298/kinneretia-thg-sqi4-mediated-biosynthesis-of-silver-nanoparticles-and-its-antimicrobial-efficacy
#3
Hina Singh, Juan Du, Tae-Hoo Yi
Simple, facile, effective approach for the biosynthesis of silver nanoparticles using Kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. Kinneretia THG-SQI4 has been isolated from soil sample collected from Shangqui, China. The synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (UV-vis), field emission transmission electron microscopy (FE-TEM), energy dispersive X-ray spectroscopy (EDX), elemental mapping, selected area diffraction pattern, and X-ray diffraction (XRD)...
May 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28207310/microbiological-quality-and-prevalence-of-%C3%AE-lactam-antibiotic-resistance-genes-in-oysters-crassostrea-rhizophorae
#4
Maria Aparecida da RessurreiÇão Brandão, Amanda Teixeira Sampaio Lopes, Maria Tereza da Silva Neta, Rhyan Barros Farias de Oliveira, Rachel Passos Rezende, George Rêgo Albuquerque, Verônica Dias Gonçalves, Dália Dos Prazeres Rodrigues, Guisla Boehs, Bianca Mendes Maciel
The microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. The objective of this study was to assess the bacterial composition of the edible oyster Crassostrea rhizophorae in urban and preserved estuaries. Particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blaTEM, blaSHV, and blaKPC) via quantitative PCR of oyster DNA...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28205102/a-simple-portable-electrochemical-biosensor-to-screen-shellfish-for-vibrio-parahaemolyticus
#5
Noordiana Nordin, Nor Azah Yusof, Jaafar Abdullah, Son Radu, Roozbeh Hushiarian
An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28169338/shrimp-ahpnd-causing-plasmids-encoding-the-pirab-toxins-as-mediated-by-pirab-tn903-are-prevalent-in-various-vibrio-species
#6
Jinzhou Xiao, Liyuan Liu, Yiyun Ke, Xiefei Li, Yunfei Liu, Yingjie Pan, Shuling Yan, Yongjie Wang
Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease caused by pirAB toxins encoded by a plasmid found in Vibrio parahaemolyticus. The pirAB toxins are the homologs of the Photorhabdus insect-related (Pir) toxins. Here, we report the complete sequences of the AHPND-causing plasmid isolated from V. owensii, as well as those of its 11 siblings (pVH family). In addition, we also included 13 related plasmids (pVH-r family) without the pirAB genes isolated from a variety of species within the Vibrio Harveyi clade...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28167526/vibrio-phage-kvp40-encodes-a-functional-nad-salvage-pathway
#7
Jae Yun Lee, Zhiqun Li, Eric S Miller
: The genome of T4-type Vibrio bacteriophage KVP40 has five genes predicted to encode proteins of pyridine nucleotide metabolism, of which two, nadV and natV, would suffice for an NAD(+) salvage pathway. NadV is an apparent nicotinamide phosphoribosyltransferase (NAmPRTase) and NatV is an apparent bifunctional nicotinamide mononucleotide adenylyltransferase (NMNATase) and nicotinamide-adenine dinucleotide pyrophosphatase (Nudix hydrolase). Genes encoding the predicted salvage pathway were cloned, expressed in E...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28166465/isolation-and-characterization-of-large-marine-bacteriophage-myoviridae-vhkm4-infecting-vibrio-harveyi
#8
Tamrin M Lal, Motohiko Sano, Julian Ransangan
The causative agent responsible for vibriosis in tropical fish aquaculture, Vibrio harveyi, has become a major bacterial pathogen. Studies suggest that this bacterium has developed resistance to antibiotics commonly used in aquaculture. In view of this situation and the requirement for the proposed postantibiotic era, bacteriophage therapy seems to be a promising control strategy for fish vibriosis. In this study, a lytic Vibrio phage VhKM4 belonging to a member of large, marine Myoviridae was successfully isolated...
March 2017: Journal of Aquatic Animal Health
https://www.readbyqxmd.com/read/28144705/ecological-fitness-and-virulence-features-of-vibrio-parahaemolyticus-in-estuarine-environments
#9
REVIEW
Charles R Lovell
Vibrio parahaemolyticus is a commonly encountered and highly successful organism in marine ecosystems. It is a fast-growing, extremely versatile copiotroph that is active over a very broad range of conditions. It frequently occurs suspended in the water column (often attached to particles or zooplankton), and is a proficient colonist of submerged surfaces. This organism is an important pathogen of animals ranging from microcrustaceans to humans and is a causative agent of seafood-associated food poisoning. This review examines specific ecological adaptations of V...
January 31, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28135311/transcriptome-analysis-reveals-comprehensive-insights-into-the-early-immune-response-of-large-yellow-croaker-larimichthys-crocea-induced-by-trivalent-bacterial-vaccine
#10
Xin Zhang, Yinnan Mu, Pengfei Mu, Jingqun Ao, Xinhua Chen
Vaccination is an effective and safe strategy for combating bacterial diseases in fish, but the mechanisms underlying the early immune response after vaccination remain to be elucidated. In the present study, we used RNA-seq technology to perform transcriptome analysis of spleens from large yellow croaker (Larimichthys crocea) induced by inactivated trivalent bacterial vaccine (Vibrio parahaemolyticus, Vibrio alginolyticus and Aeromonas hydrophila). A total of 2,789 or 1,511 differentially expressed genes (DEGs) were obtained at 24 or 72 h after vaccination, including 1,132 or 842 remarkably up-regulated genes and 1,657 or 669 remarkably down-regulated genes, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28129570/genetic-and-serological-identification-of-three-vibrio-parahaemolyticus-strains-as-candidates-for-novel-provisional-o-serotypes
#11
Xi Guo, Bin Liu, Min Chen, Yuanyuan Wang, Lu Wang, Hongyou Chen, Yao Wang, Lihong Tu, Xi Zhang, Lu Feng
Vibrio parahaemolyticus is a Gram-negative, halophilic Vibrio that naturally inhabits marine and estuarine environments worldwide and has recently been recognized as one of the most important foodborne pathogens. To date, 13 O serotypes and 71 K serotypes of V. parahaemolyticus have been identified. However, untypeable V. parahaemolyticus strains are frequently found during routine detection, indicating that other forms of serotypes exist and suggesting the necessity for extension of the antigenic scheme. In this work, through the genetic analysis of the O serotype genetic determinants (OGDs) and the production of antisera and serological tests, we identified three novel O serotypes of V...
January 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28129014/biochemical-basis-for-activation-of-virulence-genes-by-bile-salts-in-vibrio-parahaemolyticus
#12
Giomar Rivera-Cancel, Kim Orth
Bile salts act as a stressor to bacteria that transit the intestinal tract. Enteric pathogens have hijacked bile as an intestinal signal to regulate virulence factors. We recently demonstrated that Vibrio parahemolyticus senses bile salts via a heterodimeric receptor formed by the periplasmic domains of inner-membrane proteins VtrA and VtrC. Crystal structures of the periplasmic complex reveal that VtrA and VtrC form a β-barrel that binds bile salts in its hydrophobic interior to activate the VtrA cytoplasmic DNA-binding domain...
January 27, 2017: Gut Microbes
https://www.readbyqxmd.com/read/28126556/debaryomyces-hansenii-up-regulates-superoxide-dismutase-gene-expression-and-enhances-the-immune-response-and-survival-in-pacific-red-snapper-lutjanus-peru-leukocytes-after-vibrio-parahaemolyticus-infection
#13
Carlos Angulo, Minerva Maldonado, Karen Delgado, Martha Reyes-Becerril
Application of yeast is increasing to improve welfare and promotes growth in aquaculture. The halotolerant yeast Debaryomyces hansenii is normally a non-pathogenic yeast with probiotic properties and potential source of antioxidant enzymes as superoxide dismutase. Here, first, we characterized the sequence features of MnSOD and icCu/ZnSOD from Pacific red snapper, and second, we evaluated the potential antioxidant immune responses of the marine yeast Debaryomyces hansenii strain CBS004 in leukocytes which were then subjected to Vibrio parahaemolyticus infection...
January 23, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28119146/induction-of-type-i-interferons-in-response-to-bacterial-stimuli-in-large-yellow-croaker-larimichthys-crocea
#14
Yang Ding, Yanyun Guan, Jingqun Ao, Xinhua Chen
In addition to the crucial roles in coordinating antiviral immune responses, type I interferons (IFNs) also play a role in the host immunity against bacterial pathogens. Our previous study identified two type I IFNs from large yellow croaker Larimichthys croaea(Lc), LcIFNd and LcIFNh, and showed their strong induction by poly(I:C) and antiviral activities. In the present study, both LcIFNd and LcIFNh were found to be rapidly induced in head kidney and spleen by mixed bacteria of Vibrio alginolyticus, Vibrio parahaemolyticus, and Aeromonas hydrophila...
January 21, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28118133/detection-enumeration-and-isolation-of-vibrio-parahaemolyticus-and-v-vulnificus-from-seafood-development-of-a-multidisciplinary-protocol
#15
Swapan K Banerjee, Jeffrey M Farber
<em>Vibrio parahaemolyticus</em> and <em>V. vulnificus</em> are bacterial foodborne pathogens that can cause illnesses in humans after ingestion or exposure to contaminated seafood or coastal waters. A procedure that combines microbiological, biochemical, and molecular methods was designed and optimized for the detection, enumeration, isolation, and characterization of these clinically significant <em>Vibrio</em> spp. Initially, microbiological culturing is used to resuscitate and isolate presumptive <em>Vibrio</em> spp...
November 10, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28115262/biological-therapeutics-of-pongamia-pinnata-coated-zinc-oxide-nanoparticles-against-clinically-important-pathogenic-bacteria-fungi-and-mcf-7-breast-cancer-cells
#16
Balasubramanian Malaikozhundan, Baskaralingam Vaseeharan, Sekar Vijayakumar, Karuppiah Pandiselvi, Mohamed Ali Rajamohamed Kalanjiam, Kadarkarai Murugan, Giovanni Benelli
The overuse of antimicrobics and drugs has led to the development of resistance in a number of pathogens and parasites, which leads to great concerns for human health and the environment. Furthermore, breast cancer is the second most common cause of cancer death in women. MCF-7 is a widely used epithelial cancer cell line, derived from breast adenocarcinoma for in vitro breast cancer studies, since the cell line has retained several ideal characteristics particular to the mammary epithelium. In this scenario, the development of novel and eco-friendly drugs are of timely importance...
January 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28110034/transcriptome-profiles-of-the-clam-meretrix-petechialis-hepatopancreas-in-response-to-vibrio-infection
#17
Fengjuan Jiang, Xin Yue, Hongxia Wang, Baozhong Liu
Microbial diseases have received much attention due to their enormous destruction of aquaculture, and Vibrio parahaemolyticus is one of the main pathogens that cause bacterial disease in the clam Meretrix petechialis. To better understand the molecular mechanisms of the immune response to Vibrio in M. petechialis, RNA-Seq was applied to explore global expression changes of hepatopancreas from this clam after Vibrio challenge. There were 199,318,966 clean reads obtained by Illumina sequencing, which were further assembled into 214,577 transcripts, and then 147,255 unigenes with an N50 of 1393 bp were identified...
January 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28110033/purification-characterization-and-functional-analysis-of-the-immune-molecule-lectin-from-the-haemolymph-of-blue-swimmer-crab-portunus-pelagicus-and-their-antibiofilm-properties
#18
Sangily Jayanthi, Ramachandran Ishwarya, Mahalingam Anjugam, Arokiadhas Iswarya, Sivashanmugam Karthikeyan, Baskaralingam Vaseeharan
The present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab Portunus pelagicus (Pp-Lec). The Pp-Lec was purified by affinity chromatography with mannose coupled sepharose CL-4B column and it exhibits single band with a molecular weight of 155 kDa in SDS-PAGE. The surface morphology of purified Pp-Lec displays the homogeneous nature of protein. A distinct peak with a retention time of 3.3 min was appeared in high performance liquid chromatography (HPLC) and X-ray diffraction (XRD) analysis expresses a single peak at 31...
January 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28105799/molecular-characterization-and-functional-analysis-of-a-piscidin-gene-in-large-yellow-croaker-larimichthys-crocea
#19
Jing Yang, Xin-Jiang Lu, Fang-Chao Chai, Jiong Chen
The piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. In this study, we cloned piscidin-5-like type 3 (Lcpis5lt3) in large yellow croaker (Larimichthys crocea). Multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). The phylogenetic tree confirmed that Lcpis5lt3 and large yellow croaker piscidin-5-like proteins were grouped together to form a branch...
18, 2016: Zool Res
https://www.readbyqxmd.com/read/28099766/high-salinity-relaying-to-reduce-vibrio-parahaemolyticus-and-vibrio-vulnificus-in-chesapeake-bay-oysters-crassostrea-virginica
#20
Salina Parveen, Michael Jahncke, Sara Elmahdi, Helen Crocker, John Bowers, Chanelle White, Stephanie Gray, Amanda C Morris, Kathy Brohawn
Cases of Vibrio infections in the United States have tripled from 1996 to 2009 and these infections are most often associated with the consumption of seafood, particularly oysters (Crassostrea virginica). Information is needed on how to reduce numbers of Vibrio parahaemolyticus and Vibrio vulnificus in bi-valve molluscan shellfish (for example, oysters). The purpose of this study was to evaluate the effectiveness of high salinity relaying or treatment in recirculating aquaculture systems (RASs) as methods to reduce the abundance of V...
January 18, 2017: Journal of Food Science
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