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Vibrio parahaemolyticus

Songzhe Fu, Huiqin Tian, Dawei Wei, Xiaojun Zhang, Ying Liu
Acute hepatopancreatic necrosis disease (AHPND) is an emerging penaeid shrimp disease caused by Vibrio parahaemolyticus. Although V. parahaemolyticus has been isolated and sequenced from several Asia countries, the epidemiological links among the AHPND outbreaks in different locations remain unclear. In this study, we sequenced the genomes of nine strains isolated in China between 2014 and 2016 from four sampling sites in three provinces. Analysis of single nucleotide polymorphisms (SNPs) among the nine isolates yielded an average of 35,519 SNPs per isolate, ranging from 35,001 SNPs to 35,889 SNPs relative to the reference genome FDA_R31...
2017: Frontiers in Microbiology
Ming Li, Chunxia Ma, Haoyang Li, Jinxia Peng, Digang Zeng, Xiaohan Chen, Chaozheng Li
Antimicrobial peptides (AMPs) are the most important players in the innate immune system, providing a principal first-line of defense against the invading pathogens. Crustin, a type of whey acidic protein (WAP) domain-containing and cationic cysteine-rich AMP, can function in a protease inhibition or an effector molecule manner. In the present study, a new Crustin was cloned and identified from Pacific white shrimp Litopenaeus vannamei and designated as LvCrustinA. The full-length cDNA of LvCrustinA was 687 bp, with a 519 bp open reading frame (ORF) that encoded a peptide of 172 amino acids...
December 5, 2017: Fish & Shellfish Immunology
Vairavan Girija, Balasubramanian Malaikozhundan, Baskaralingam Vaseeharan, Sekar Vijayakumar, Narayanan Gopi, Marian Del Valle Herrera, Jiann-Chu Chen, Perumal Santhanam
In vitro antagonistic activity and the protective effect of probiotic Bacillus licheniformis Dahb1 in zebrafish (Danio rerio) challenged with GFP tagged Vibrio parahaemolyticus Dahv2 was studied. The cell free extract of probiotic B. licheniformis Dahb1 at 100 μg mL-1 showed growth inhibition of V. parahaemolyticus Dahv2 in vitro. B. licheniformis Dahb1 also inhibited the biofilm growth of GFP tagged V. parahaemolyticus Dahv2 at 100 μg mL-1 in vitro. The growth and survival of zebrafish was tested using probiotic B...
November 30, 2017: Microbial Pathogenesis
Shin-Jen Lin, Kai-Cheng Hsu, Hao-Ching Wang
In aquaculture, shrimp farming is a popular field. The benefits of shrimp farming include a relatively short grow-out time, high sale price, and good cost recovery. However, outbreaks of serious diseases inflict serious losses, and acute hepatopancreatic necrosis disease (AHPND) is an emerging challenge to this industry. In South American white shrimp (Penaeus vannamei) and grass shrimp (Penaeus monodon), this disease has a 70-100% mortality. The pathogenic agent of AHPND is a specific strain of Vibrio parahaemolyticus which contains PirAvp and PirBvp toxins encoded in the pVA1 plasmid...
December 1, 2017: Marine Drugs
Benjaporn Phupet, Thanawat Pitakpornpreecha, Nuntaporn Baowubon, Phanthipha Runsaeng, Prapaporn Utarabhand
Lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) existed in diversity of invertebrates including shrimp plays a crucial role in an innate immunity via mediating the recognition of invading pathogens. In this study, LGBP was cloned and characterized from the hepatopancreas of Litopenaeus vannamei, named as LvLGBP. Its full-length cDNA of 1282 bp contained an open reading frame (1101 bp) encoding a peptide of 367 amino acids. The LGBP primary structure contained a glycosyl hydrolase domain, two integrin binding motifs, two kinase C phosphorylation sites, and two polysaccharide recognition motifs which were identified as a polysaccharide binding motif and a β-1,3-glucan recognition motif...
November 27, 2017: Developmental and Comparative Immunology
Qingsong Huang, Mingjia Yu, Hongmei Chen, Manhong Zeng, Yan Sun, Tusar T Saha, Dongbo Chen
In bivalve mollusks, circulating hemocyte mediated phagocytosis is one of the primary ways to eliminate invading microbes. Here, we have identified one CgLRFN (leucine-rich repeat and fibronectin type-III domain-containing protein) in the Crassostrea gigas as a novel transmembrane LRR (Leucine-rich repeat) domain containing protein in C. gigas, homologous to the jawless fish VLR protein, that plays an important role in recognizing bacteria and promoting hemocytic phagocytosis. Tissue distribution analysis of CgLRFN in Pacific oyster showed that it is widely expressed in various tissues like the gills, adductor muscles, digestive glands, gonads, heart and in the hemocytes...
November 27, 2017: Fish & Shellfish Immunology
Xiaoxi Zhang, Xiaojun Zhang, Jianbo Yuan, Jiangli Du, Fuhua Li, Jianhai Xiang
Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs belong to the slow-type skeletal muscle actins, and 18 belong to the fast-type skeletal muscle actins; they were designated LvActinSSK and LvActinFSK, respectively...
November 30, 2017: Molecular Genetics and Genomics: MGG
Xiaoxia Wang, Wei Xu, Huihui Zhou, Yanjiao Zhang, Weihua Gao, Wenbing Zhang, Kangsen Mai
The present study was conducted to investigate the effects of reduced glutathione (GSH) supplementation in practical diet on growth performance, anti-oxidative response, disease resistance and intestine morphology of shrimp Litopenaeus vannamei. Two control diets based on the commercial formulation were designed with high level (27%) and low level (22%) of fish meal, respectively. Based on the control diet with low level of fish meal, 75, 150 and 225 mg/kg of GSH were added, respectively, to make the other three experimental diets...
November 25, 2017: Fish & Shellfish Immunology
Le Dinh Hung, Bui Minh Ly, Vo Thi Hao, Dinh Thanh Trung, Vo Thi Dieu Trang, Phan Thi Hoai Trinh, Ngo Thi Duy Ngoc, Thai Minh Quang
SFL, a lectin from the marine sponge Stylissa flexibilis was purified by cold ethanol precipitation followed by ion exchange chromatography on DEAE Sepharose column and Sephacryl S-200 gel filtration. SFL is a dimeric glycoprotein of 32kDa subunits linked by a disulfide bridge with a molecular mass of 64kDa by SDS-PAGE and 65kDa by Sephacryl S-200 gel filtration. SFL preferentially agglutinated enzyme treated human A erythrocytes. The activity of lectin was strongly inhibited by monosaccharide d-galactose and glycoproteins asialo-porcine stomach mucin and asialo-fetuin...
November 22, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Ji-Fang Yin, Ming-Yi Wang, Ying-Jian Chen, Hong-Qiu Yin, Yan Wang, Ming-Qiang Lin, Ai-Yan Liu, Cheng-Jin Hu
BACKGROUND: Vibrio vulnificus, V. alginolyticus, and V. parahaemolyticus are commonly and opportunistically pathogenic to humans. METHODS: In this study, a novel multiple touchdown polymerase chain reaction method (MT-PCR) was developed to benefit rapid and simultaneous detection of the presence of the three Vibrio species from the enriched clinical and environmental samples. RESULTS: The method showed a sensitivity of 104 colony forming units (CFU)/mL for V...
November 27, 2017: Surgical Infections
Jesús A Pérez-Acosta, Marcel Martínez-Porchas, José M Elizalde-Contreras, Juan Manuel Leyva, Eliel Ruiz-May, Teresa Gollas-Galván, Luis R Martínez-Córdova, José Ángel Huerta-Ocampo
Vibrio parahaemolyticus has been recognized as causal agent of the early mortality syndrome and is currently considered an emerging shrimp disease causing millionaire loses to aquaculture industry. Integral membrane proteins are widely recognized as pathogenicity factors involved in essential mechanisms which allow V. parahaemolyticus infection and make them attractive as therapeutic targets. However, physico-chemical properties and low expression levels results usually into underrepresentation of these proteins in conventional bottom-up proteomic, making integral membrane proteomics a challenging task...
November 24, 2017: Microbiology and Immunology
Chutima Tanapichatsakul, Sakon Monggoot, Eleni Gentekaki, Patcharee Pripdeevech
Fifty-two strains of endophytic fungi were isolated from flowers of the medicinal plant Melodorum fruticosum. Seven genera were identified including Alternaria, Aspergillus, Colletotrichum, Diaporthe, Fusarium, Greeneria and Nigrospora. All strains were cultured for 30 days and further macerated in ethyl acetate solvent for 3 days. The obtained fungal extracts were examined for antibacterial activity using agar disc diffusion against nine pathogenic bacteria: Staphylococcus aureus, Bacillus subtilis, B. cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Shigella flexneri, Vibrio cholerae and V...
November 20, 2017: Current Microbiology
Mohammad M Obaidat, Alaa E Bani Salman, Amira A Roess
Vibrio parahaemolyticus is a leading cause of seafood-associated illness. This study investigated the prevalence, virulence, and antibiotic resistance of V. parahaemolyticus in three low- and middle-income countries. Freshly caught fish samples (n = 330) imported to Jordan from Yemen, India, and Egypt were tested. The overall prevalence of V. parahaemolyticus was 15% (95% confidence interval: 11 to 19%). Three isolates (6%) were positive for the thermostable direct hemolysin-related (trh) gene, and all isolates was negative for the thermostable direct hemolysin (tdh) gene...
November 20, 2017: Journal of Food Protection
Sara Elmahdi, Salina Parveen, Sylvia Ossai, Ligia V DaSilva, Michael Jahncke, John Bowers, John Jacobs
Vibrio parahaemolyticus (Vp) and Vibrio vulnificus (Vv) are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the USA. Though multiple-antibiotic resistant Vp and Vv have been reported resistance patterns in vibrios are not as well documented as other food-borne bacterial pathogens. Salinity relaying (SR) is a Post-Harvest Processing (PHP) treatment to reduce the abundance of these pathogens in shellfish harvested during the warmer months. The purpose of this study was to evaluate the antimicrobial susceptibility (AMS), pathogenicity and genetic profiles of Vp and Vv recovered from oysters during an oyster relay study...
November 17, 2017: Applied and Environmental Microbiology
Ryu Okada, Shigeaki Matsuda, Tetsuya Iida
Vibrio parahaemolyticus is a Gram-negative pathogen that causes food-borne gastroenteritis. A major virulence determinant of the organism is a type III secretion system (T3SS2) encoded on a pathogenicity island, Vp-PAI. Vp-PAI gene expression is regulated by two transcriptional regulators, VtrA and VtrB, whose N-terminal regions share homology with an OmpR-family DNA-binding domain. VtrA activates the gene expression of VtrB, which in turn activates Vp-PAI gene expression; however, the mechanism of this transcriptional activation by VtrA is not well understood...
2017: PloS One
Alessandro Delli Paoli Carini, Ellen Ariel, Jacqueline Picard, Lisa Elliott
This study aimed to test multidrug resistant isolates from hospitalised green turtles (Chelonia mydas) and their environment in North Queensland, Australia, for in vitro susceptibility to bacteriophages. Seventy-one Gram-negative bacteria were isolated from green turtle eye swabs and water samples. Broth microdilution tests were used to determine antibiotic susceptibility. All isolates were resistant to at least two antibiotics, with 24% being resistant to seven of the eight antibiotics. Highest resistance rates were detected to enrofloxacin (77%) and ampicillin (69...
2017: International Journal of Microbiology
Arivalagan Pugazhendhi, Desika Prabakar, Jaya Mary Jacob, Indira Karuppusamy, Rijuta Ganesh Saratale
Microfouling is evolving at a fast rate causing augmented mortality rates and damage worldwide. Until now, several remedial measures have been exploited to overcome microfouling, amongst them nanoparticles play a superior role. Currently, green synthesized nanoparticles have been centered owing to its eco-friendly, cost effectively and non-toxic nature which has also increased its industrial applications (biomedicine, food and textile). In the present research Silver Nanoparticles (Ag NPs) synthesized using marine red algae Gelidium amansii...
November 13, 2017: Microbial Pathogenesis
Guang Yang, Yunji Xiu, Yadong Chen, Li Bai, Zhenxia Sha
Complement component C8, which mediates membrane attack complex formation and bacterial lysis, plays important roles in the complement system. The cDNA sequences of the C8α, C8β and C8γ genes were cloned from half-smooth tongue sole (Cynoglossus semilaevis). Full-length cDNA of CsC8α (C8α of C. semilaevis), CsC8β and CsC8γ was 1990, 2219 and 886 bp, respectively, which contained open reading frames of 1797, 1749 and 666 bp, encoding 598, 582 and 221 amino acids, respectively. The deduced proteins of CsC8α, CsC8β and CsC8γ showed the closest amino acid similarity to C8α (73%) of Siniperca chuatsi, C8β (76%) of Oryzias latipes and C8γ (72%) of Takifugu rubripes, respectively...
November 13, 2017: Fish & Shellfish Immunology
Defu Yao, Huimin Su, Jinghua Zhu, Xianliang Zhao, Jude Juventus Aweya, Fan Wang, Mingqi Zhong, Yueling Zhang
Tolls and Toll-like receptors (TLRs) are important regulators in the innate immune system and their genetic variations usually affect the host's susceptibility/resistance to pathogen infections. In this study, we report on the single nucleotide polymorphisms (SNPs) of Toll1 in Litopenaeus vannamei (LvToll1) and how this is associated with immune response. PCR-DGGE analysis revealed genetic polymorphisms in LvToll1 at both the genomic DNA (gDNA) and cDNA levels. Using high-throughput sequencing, 223 SNPs were identified at the gDNA level, of which 145 were non-synonymous SNP (nsSNP), with 3 nsSNPs having frequency over 1%...
November 13, 2017: Fish & Shellfish Immunology
Hongkuan Zhang, Dewei Cheng, Hongxing Liu, Huaiping Zheng
Being lack of specific immune system, both enzymes and non-enzymatic antioxidants play crucial roles in immune of invertebrates. In the present study, in order to investigate immune roles of enzyme (thioredoxin, TRX) and antioxidants (carotenoids), Golden scallops with golden shell and golden muscle rich in carotenoids content and Brown scallops with brown shell and white muscle less carotenoids content of the noble scallop Chlamys nobilis were challenged by Vibrio parahaemolyticus for 48 h. Firstly, a cDNA of TRX protein gene from the scallop (named as CnTRX) was cloned and characterized...
November 13, 2017: Fish & Shellfish Immunology
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