keyword
MENU ▼
Read by QxMD icon Read
search

Vibrio parahaemolyticus

keyword
https://www.readbyqxmd.com/read/28648882/transcriptome-analysis-of-the-hepatopancreas-in-exopalaemon-carinicauda-infected-with-an-ahpnd-causing-strain-of-vibrio-parahaemolyticus
#1
Qianqian Ge, Jian Li, Jiajia Wang, Jitao Li, Hongxing Ge, Qianqian Zhai
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in multiple shrimp species throughout Asia. In order to understand the immunological response of shrimp to infection by AHPND-causing strain of V. parahaemolyticus (VPAHPND), the transcriptomic profiles of the hepatopancreas from severe AHPND-infected (BS_G), AHPND-survived (KB_G) and non-infected (PBS_G) Exopalaemon carinicauda groups were obtained using HiSeq™ 2500 (Illumina)...
June 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28646477/a-double-quadratic-model-for-predicting-vibrio-species-in-water-environments-of-japan
#2
Hidemasa Izumiya, Masato Furukawa, Kikuyo Ogata, Junko Isobe, Setsu Watanabe, Mari Sasaki, Kazuya Ichinose, Eiji Arakawa, Masatomo Morita, Ichiro Kurane, Makoto Ohnishi
Vibrio spp. are natural inhabitants of marine and estuarine environments. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the major infectious agents for humans. Their densities are affected by environmental factors such as water temperature and salinity. The detailed contribution of each factor still remains to be elucidated. Here we conducted multi-coastal study in a 21-month period to examine relationships between environmental factors and V. cholerae, V. parahaemolyticus and V. vulnificus densities in sea surface water in eight coastal sites of four prefectures in Japan...
June 23, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28642752/a-comprehensive-epidemiological-research-for-clinical-vibrio-parahaemolyticus-in-shanghai
#3
Huan Li, Rong Tang, Yang Lou, Zelin Cui, Wenjing Chen, Qing Hong, Zhaohuan Zhang, Pradeep K Malakar, Yingjie Pan, Yong Zhao
Vibrio parahaemolyticus is one of the most important pathogen for seafood-borne gastroenteritis in Shanghai and the rest of the world. A total of 42 V. parahaemolyticus strains were isolated from 1900 fecal specimens collected from patients in Shanghai hospital presenting from January 2014 to December 2015. All isolates were evaluated for potential virulence factors [tdh, trh, and type three secretion system (T3SS) genes], typed using multilocus sequence typing (MLST) and screened for antimicrobial resistance phenotype and genotype...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28640881/t3ss-effector-vopl-inhibits-the-host-ros-response-promoting-the-intracellular-survival-of-vibrio-parahaemolyticus
#4
Marcela de Souza Santos, Dor Salomon, Kim Orth
The production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (NADPH) oxidase complex is an important mechanism for control of invading pathogens. Herein, we show that the gastrointestinal pathogen Vibrio parahaemolyticus counteracts reactive oxygen species (ROS) production using the Type III Secretion System 2 (T3SS2) effector VopL. In the absence of VopL, intracellular V. parahaemolyticus undergoes ROS-dependent filamentation, with concurrent limited growth. During infection, VopL assembles actin into non-functional filaments resulting in a dysfunctional actin cytoskeleton that can no longer mediate the assembly of the NADPH oxidase at the cell membrane, thereby limiting ROS production...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28638370/removal-of-foodborne-pathogen-biofilms-by-acidic-electrolyzed-water
#5
Qiao Han, Xueying Song, Zhaohuan Zhang, Jiaojiao Fu, Xu Wang, Pradeep K Malakar, Haiquan Liu, Yingjie Pan, Yong Zhao
Biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (EPS), are difficult to remove in food production facilities. In this study, the use of acidic electrolyzed water (AEW) to remove foodborne pathogen biofilms was evaluated. We used a green fluorescent protein-tagged Escherichia coli for monitoring the efficiency of AEW for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm disappeared rapidly and the population of biofilm cells was reduced by more than 67%...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28630016/comparative-transcriptome-analysis-of-sinonovacula-constricta-in-gills-and-hepatopancreas-in-response-to-vibrio-parahaemolyticus-infection
#6
Xuelin Zhao, Xuemei Duan, Zhenhui Wang, Weiwei Zhang, Ye Li, Chunhua Jin, Jinbo Xiong, Chenghua Li
The razor clam Sinonovacula constricta is an important economic species in China. However, bacterial pathogenic diseases limits S. constricta farming industry for large-scale production. In this study, de novo transcriptome sequencing was performed on S. constricta gills and hepatopancreas under Vibrio parahaemolyticus challenge for 12 h and 48 h, respectively. Transcripts assembly constructed 18,330 sequences, each of which was 500 bp long and functionally annotated, and 1781 and 490 transcripts were differentially expressed in the gills and hepatopancreas, respectively...
June 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28625840/survival-of-litopenaeus-vannemei-shrimp-fed-on-diets-supplemented-with-dunaliella-sp-is-improved-after-challenges-by-vibrio-parahaemolyticus
#7
Diana Medina Félix, José Antonio López Elías, Ángel Isidro Campa Córdova, Luis Rafael Martínez Córdova, Antonio Luna González, Edilmar Cortez Jacinto, Nolberta Huerta Aldaz, Fernando Cano Mendoza, María Guadalupe Burboa Zazueta
Survival of Litopenaeus vannamei was evaluated during a Vibrio parahaemolyticus infection. This bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (EMS), also known as acute hepatopancreatic necrosis disease (AHPND). Control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. New methods have been proposed to control and prevent the impact of bacterial infections. The physiological response indicated by plasma biochemical parameters in shrimp can determine their health and stress status...
June 15, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28621716/antibacterial-and-antifungal-activities-of-spices
#8
REVIEW
Qing Liu, Xiao Meng, Ya Li, Cai-Ning Zhao, Guo-Yi Tang, Hua-Bin Li
Infectious diseases caused by pathogens and food poisoning caused by spoilage microorganisms are threatening human health all over the world. The efficacies of some antimicrobial agents, which are currently used to extend shelf-life and increase the safety of food products in food industry and to inhibit disease-causing microorganisms in medicine, have been weakened by microbial resistance. Therefore, new antimicrobial agents that could overcome this resistance need to be discovered. Many spices-such as clove, oregano, thyme, cinnamon, and cumin-possessed significant antibacterial and antifungal activities against food spoilage bacteria like Bacillus subtilis and Pseudomonas fluorescens, pathogens like Staphylococcus aureus and Vibrio parahaemolyticus, harmful fungi like Aspergillus flavus, even antibiotic resistant microorganisms such as methicillin resistant Staphylococcus aureus...
June 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28606862/pathogenicity-profile-of-vibrio-parahaemolyticus-in-farmed-pacific-white-shrimp-penaeus-vannamei
#9
R Ananda Raja, R Sridhar, C Balachandran, A Palanisammi, S Ramesh, K Nagarajan
A pathobiological study was conducted using Vibrio parahaemolyticus (VP) strain isolated from vibriosis affected shrimp (Penaeus vannamei) farms in Kancheepuram and Thiruvallur districts of Tamil Nadu during August 2014 to February 2015. The isolate was identified based on the morphological, physiological, biochemical and molecular characters. LD50 value with intramuscular injection was determined as 2.6 × 10(4) cfu/shrimp and sequential pathology was studied giving 6.1 × 10(3) cfu/shrimp (LD25). Total plate count (TPC) and total Vibrio count (TVC) in water, pond sediment, haemolymph, muscle, HP and gut were found significantly (P < 0...
June 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28602685/isolation-and-characterization-of-antimicrobial-peptides-derived-from-bacillus-subtilis-e20-fermented-soybean-meal-and-its-use-for-preventing-vibrio-infection-in-shrimp-aquaculture
#10
Ann-Chang Cheng, Hsueh-Li Lin, Ya-Li Shiu, Yu-Chang Tyan, Chun-Hung Liu
Bacillus subtilis E20-fermented soybean meal (FSBM) was found to produce antimicrobial peptides (AMPs) with great antimicrobial activity against Vibrio alginolyticus (VA) and V. parahaemolyticus (VP). Three AMPs were purified with a 5 kDa ultrafiltration, Sephadex G-15 column and reverse-phase high-performance liquid chromatography (RP-HPLC). The FSB-AMP, HTSKALLDMLKRLGK, identified by an RP-nano-ultrapure liquid chromatography (UPLC) electrospray ionization (ESI)-tandem mass spectroscopic (MS/MS) analysis exhibited the highest bactericidal activity against VA and VP compared to the others...
June 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28585379/groel-is-a-suitable-genetic-marker-for-detecting-vibrio-parahaemolyticus-by-loop-mediated-isothermal-amplification-lamp-assay
#11
Mahbubul Pratik Siddique, Won Je Jang, Jong Min Lee, Sun-Hee Ahn, Sharmin Suraiya, Chang-Hoon Kim, In-Soo Kong
A groEL gene-based loop-mediated isothermal amplification (LAMP) assay was developed to detect Vibrio parahaemolyticus in contaminated seafood and water. The assay was optimized and conducted at 63°C for 40 min using Bacillus stearothermophilus (Bst) DNA polymerase, large fragment. Amplification was analyzed via multiple detection methods, including opacity, formation of white precipitate, DNA intercalating dyes (ethidium bromide and SYBR Green I), metal ion-binding indicator dye, calcein, and 2% agarose gel electrophoresis...
June 5, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28583064/genomic-and-transcriptomic-analyses-reveal-distinct-biological-functions-for-cold-shock-proteins-vpacspa-and-vpacspd-in-vibrio-parahaemolyticus-chn25-during-low-temperature-survival
#12
Chunhua Zhu, Boyi Sun, Taigang Liu, Huajun Zheng, Wenyi Gu, Wei He, Fengjiao Sun, Yaping Wang, Meicheng Yang, Weicheng Bei, Xu Peng, Qunxin She, Lu Xie, Lanming Chen
BACKGROUND: Vibrio parahaemolyticus causes serious seafood-borne gastroenteritis and death in humans. Raw seafood is often subjected to post-harvest processing and low-temperature storage. To date, very little information is available regarding the biological functions of cold shock proteins (CSPs) in the low-temperature survival of the bacterium. In this study, we determined the complete genome sequence of V. parahaemolyticus CHN25 (serotype: O5:KUT). The two main CSP-encoding genes (VpacspA and VpacspD) were deleted from the bacterial genome, and comparative transcriptomic analysis between the mutant and wild-type strains was performed to dissect the possible molecular mechanisms that underlie low-temperature adaptation by V...
June 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28577391/shrimp-tab1-interacts-with-tak1-and-p38-and-activates-the-host-innate-immune-response-to-bacterial-infection
#13
Sheng Wang, Mengqiao Li, Bin Yin, Haoyang Li, Bang Xiao, Kai Lǚ, Zhijian Huang, Sedong Li, Jianguo He, Chaozheng Li
Mammalian TAB1 has been previously identified as transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1) binding protein, which functions as the activator of TAK1 and p38. This report, for the first time, identified and characterized the homolog of TAB1 in shrimp, to be specific, the homolog gene from Litopenaeus vannamei, containing a 1560-bp open reading frame (ORF) that encoded a putative protein of 519 amino acids with the conserved PP2Cc (Serine/threonine phosphatases, family 2C, catalytic) domain in N-terminal and a TAK1 binding motif in C-terminus, has been cloned and named LvTAB1...
May 31, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28576761/a-natural-vibrio-parahaemolyticus-pir-vp-a-b-mutant-kills-shrimp-but-produces-no-pir-vp-toxins-or-ahpnd-lesions
#14
Kornsunee Phiwsaiya, Walaiporn Charoensapsri, Suwimon Taengphu, Ha T Dong, Pakkakul Sangsuriya, Giang T T Nguyen, Hung Q Pham, Piti Amparyup, Kallaya Sritunyalucksana, Suparat Taengchaiyaphum, Parin Chaivisuthangkura, Siwaporn Longyant, Paisarn Sithigorngul, Saengchan Senapin
Acute hepatopancreatic necrosis disease (AHPND) of shrimp is caused by Vibrio parahaemolyticus (VP) isolates that harbor a pVA plasmid encoding toxins Pir(vp)A and Pir(vp)B (VPAHPND). These are released from VPAHPND that colonize the shrimp stomach and produce pathognomonic AHPND lesions (massive sloughing of hepatopancreatic tubule epithelial cells). PCR results indicated that VP isolate XN87 lacked Pir(vp)A but carried Pir(vp)B Unexpectedly, western blot analysis of proteins from culture broth of XN87 revealed absence of both toxins and the lack of Pir(vp)B was further confirmed by ELISA assay...
June 2, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28573108/spreading-of-pandemic-vibrio-parahaemolyticus-o3-k6-and-its-serovariants-a-re-analysis-of-strains-isolated-from-multiple-studies
#15
Dongsheng Han, Fei Yu, Hui Tang, Chuanli Ren, Caiyun Wu, Pan Zhang, Chongxu Han
In China, V. parahaemolyticus has been a leading cause of foodborne outbreaks and bacterial infectious diarrhea since the 1990s, and most infections have been associated with the pandemic V. parahaemolyticus O3:K6 and its serovariants. However, a comprehensive overview of the sero-prevalence and genetic diversity of the pandemic V. parahaemolyticus clone in China is lacking. To compensate for this deficiency, pandemic isolates in both clinical and environmental Chinese samples collected from multiple studies were analyzed in this study...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28570527/induction-of-cellular-differentiation-and-single-cell-imaging-of-vibrio-parahaemolyticus-swimmer-and-swarmer-cells
#16
Jan Heering, Alejandra Alvarado, Simon Ringgaard
The ability to study the intracellular localization of proteins is essential for the understanding of many cellular processes. In turn, this requires the ability to obtain single cells for fluorescence microscopy, which can be particularly challenging when imaging cells that exist within bacterial communities. For example, the human pathogen Vibrio parahaemolyticus exists as short rod-shaped swimmer cells in liquid conditions that upon surface contact differentiate into a subpopulation of highly elongated swarmer cells specialized for growth on solid surfaces...
May 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28553617/bioluminescence-imaging-to-track-bacteroides-fragilis-inhibition-of-vibrio-parahaemolyticus-infection-in-mice
#17
Zhengchao Li, Huimin Deng, Yazhou Zhou, Yafang Tan, Xiaoyi Wang, Yanping Han, Yangyang Liu, Ye Wang, Ruifu Yang, Yujing Bi, Fachao Zhi
Bacteroides fragilis is an anaerobic, Gram-negative, commensal bacterium of the human gut. It plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. Many recent studies have focused on the relationship between B. fragilis and human immunity, and indicate that B. fragilis has many useful probiotic effects. As inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether B. fragilis could inhibit pathogenic bacteria...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28533087/a-rele-pare-superfamily-toxin-in-vibrio-parahaemolyticus-has-dna-nicking-endonuclease-activity
#18
Jing Zhang, Hironori Ito, Madoka Hino, Makoto Kimura
Type II toxins in toxin-antitoxin (TA) systems fold into a similar fold and belong to the RelE/ParE superfamily. However, they display two distinct biochemical activities: RelE toxins are mRNA interferases, while ParE toxins are DNA gyrase (Gyr) inhibitors. Previously, we found a TA system, vp1842/vp1843, on the Vibrio parahaemolyticus genome whose toxin Vp1843 belongs to the RelE/ParE toxin superfamily. Vp1843, unlike RelE toxins, has neither protein synthesis inhibitory activity nor ribonuclease activity...
July 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28531284/different-abundance-and-correlational-patterns-exist-between-total-and-presumed-pathogenic-vibrio-vulnificus-and-v-parahaemolyticus-in-shellfish-and-waters-along-the-north-carolina-coast
#19
Tiffany C Williams, Brett A Froelich, Britney Phippen, Patricia Fowler, Rachel T Noble, James D Oliver
Monitoring of Vibrio vulnificus and V. parahaemolyticus abundance is pertinent due to the ability of these species to cause disease in humans through aquatic vectors. Previously, we performed a multiyear investigation tracking Vibrio spp. levels in five sites along the southeastern North Carolina coast. From February 2013 to October 2015, total V. vulnificus and V. parahaemolyticus abundance was measured in water, oysters and clams. In the current study, pathogenic subpopulations were identified in these isolates using molecular markers, revealing that 5...
June 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28525966/gene-expression-of-vibrio-parahaemolyticus-growing-in-laboratory-isolation-conditions-compared-to-those-common-in-its-natural-ocean-environment
#20
Katherine García, Cristian Yáñez, Nicolás Plaza, Francisca Peña, Pedro Sepúlveda, Diliana Pérez-Reytor, Romilio T Espejo
BACKGROUND: Vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °C, a condition seldom found in the ocean. However, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. In this context, revealing how gene expression enables V. parahaemolyticus to adapt to this particular condition -common to almost all V. parahaemolyticus isolates- will improve our understanding of the biology of this important pathogen...
May 19, 2017: BMC Microbiology
keyword
keyword
88762
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"