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Vibrio parahaemolyticus

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https://www.readbyqxmd.com/read/27932023/molecular-cloning-and-expression-analysis-of-a-fish-specific-interferon-regulatory-factor-irf11-in-orange-spotted-grouper-epinephelus-coioides
#1
Wen Shu Huang, Mei Hua Zhu, Shan Chen, Zhi Xuan Wang, Ying Liang, Bei Huang, P Nie
Interferon regulatory factors (IRFs) are transcription mediators which play vital roles in multiple biological processes, such as antiviral defense, immune response, cell growth regulation and apoptosis. A fish specific IRF, termed IRF11, has been identified in previous study through searching fish genome databases. Herein, a transcript of IRF11, EcIRF11 was cloned from orange-spotted grouper, Epinephelus coioides. The EcIRF11 cDNA sequence has 1573 bp in length, encoding a putative protein of 261 amino acids, with a high degree of similarity found between EcIRF11 and its teleost counterparts...
December 5, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27923189/sensitive-immunoassay-based-detection-of-vibrio-parahaemolyticus-using-capture-and-labeling-particles-in-a-stationary-liquid-phase-lab-on-a-chip
#2
Byunghee Park, Suk-Jung Choi
In the present study, a method was developed for detection of Vibrio parahaemolyticus based on a stationary liquid phase lab-on-a-chip (SLP LOC). The present SLP LOC comprises a sample chamber, washing chamber, and detection chamber connected by two channels. The method utilizes two types of particles: capture particles (CPs), which are magnetic nanoparticles functionalized with antibody; and labeling particles (LPs), which are silica nanoparticles functionalized with horseradish peroxidase and antibody. Samples were added to the sample chamber with CPs and LPs, forming a CP-bacteria-LP complex, and the complex was transported to the detection chamber containing chromogenic substrate solution...
December 1, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27922123/lptd-is-a-promising-vaccine-antigen-and-potential-immunotherapeutic-target-for-protection-against-vibrio-species-infection
#3
Zhenzhong Zha, Chuchu Li, Weiyan Li, Zhicang Ye, Jianyi Pan
Outer membrane proteins (OMPs) are unique to Gram-negative bacteria. Several features, including surface exposure, conservation among strains and ability to induce immune responses, make OMPs attractive targets for using as vaccine antigens and immunotherapeutics. LptD is an essential OMP that mediates the final transport of lipopolysaccharide (LPS) to outer leaflet. The protein in Vibrio parahaemolyticus was identified to have immunogenicity in our previous report. In this study, broad distribution, high conservation and similar surface-epitopes of LptD were found among the major Vibrio species...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27919756/molecular-identification-and-functional-delineation-of-a-glutathione-reductase-homologue-from-disk-abalone-haliotis-discus-discus-insights-as-a-potent-player-in-host-antioxidant-defense
#4
H M L P B Herath, P D S U Wickramasinghe, S D N K Bathige, R G P T Jayasooriya, Gi-Young Kim, Myoung Ae Park, Chul Kim, Jehee Lee
Glutathione reductase (GSR) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (GSSG) into the reduced form (GSH). Since the ratio between the two forms of glutathione (GSH/GSSG) is important for the optimal function of GSH to act as an antioxidant against H2O2, the contribution of GSR as an enzymatic regulatory agent to maintain the proper ratio is essential. Abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduction of reactive oxygen species (ROS) such as H2O2...
December 2, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27911360/development-of-a-more-sensitive-and-specific-chromogenic-agar-medium-for-the-detection-of-vibrio-parahaemolyticus-and-other-vibrio-species
#5
Marie Yeung, Trevor Thorsen
Foodborne infections in the US caused by Vibrio species have shown an upward trend. In the genus Vibrio, V. parahaemolyticus is responsible for the majority of Vibrio-associated infections. Thus, accurate differentiation among Vibrio spp. and detection of V. parahaemolyticus is critically important to ensure the safety of our food supply. Although molecular techniques are increasingly common, culture-depending methods are still routinely done and they are considered standard methods in certain circumstances...
November 8, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27903451/fatty-acid-synthase-plays-a-positive-role-in-shrimp-immune-responses-against-vibrio-parahaemolyticus-infection
#6
Hongliang Zuo, Jiefeng Gao, Jia Yuan, Hengwei Deng, Linwei Yang, Shaoping Weng, Jianguo He, Xiaopeng Xu
Fatty acid synthase (FAS) is an important enzyme that catalyzes the synthesis of fatty acids. In this study, the role of the FAS gene from pacific white shrimp Litopenaeus vannamei (LvFAS) in immune responses against Vibrio parahaemolyticus infection was studied. The expression of LvFAS could be up-regulated upon infection of V. parahaemolyticus and stimulation of lipopolysaccharide and poly (I:C). The promoter of LvFAS was predicted to harbor a NF-κB binding site and dual-luciferase reporter assays demonstrated that the NF-κB family proteins Relish, sRelish and Dorsal could activate the transcription of LvFAS...
November 27, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27901504/evaluation-of-medicinal-plants-and-colloidal-silver-efficiency-against-vibrio-parahaemolyticus-infection-in-litopenaeus-vannamei-cultured-at-low-salinity
#7
María Soledad Morales-Covarrubias, Noemí García-Aguilar, María Del Bolan-Mejía, Ana Carmela Puello-Cruz
In shrimp aquaculture, reduction in the use of synthetic antibiotics is a priority due to the high incidence of resistant bacteria (Vibrio) in the white shrimp Litopenaeus vannamei. An increasing number of studies show bactericidal activity of natural treatments in aquaculture. The effectiveness of neem (Azadirachta indica) and oregano (Lippia berlandieri) aqueous extracts and colloidal silver against V. parahaemolyticus were evaluated in low salinity shrimp culture. Results show that aqueous extracts of oregano and neem each present a minimum inhibitory concentration (MIC) of 62...
November 22, 2016: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/27891183/complete-genome-of-vibrio-parahaemolyticus-forc014-isolated-from-the-toothfish
#8
Sojin Ahn, Han Young Chung, Sooyeon Lim, Kwondo Kim, Suyeon Kim, Eun Jung Na, Kelsey Caetano-Anolles, Ju-Hoon Lee, Sangryeol Ryu, Sang Ho Choi, Heebal Kim
BACKGROUND: Foodborne illness can occur due to various pathogenic bacteria such as Staphylococcus aureus, Escherichia coli and Vibrio parahaemolyticus, and can cause severe gastroenteritis symptoms. In this study, we completed the genome sequence of a foodborne pathogen V. parahaemolyticus FORC_014, which was isolated from suspected contaminated toothfish from South Korea. Additionally, we extended our knowledge of genomic characteristics of the FORC_014 strain through comparative analysis using the complete sequences of other V...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27891182/case-control-study-of-diarrheal-disease-etiology-in-individuals-over-5%C3%A2-years-in-southwest-china
#9
Shun-Xian Zhang, Chun-Li Yang, Wen-Peng Gu, Lin Ai, Emmanuel Serrano, Pin Yang, Xia Zhou, Shi-Zhu Li, Shan Lv, Zhi-Sheng Dang, Jun-Hu Chen, Wei Hu, Li-Guang Tian, Jia-Xu Chen, Xiao-Nong Zhou
BACKGROUND: Acute diarrhea is one of the major public health problems worldwide. Most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in China. METHODS: 271 diarrhea cases and 149 healthy controls over 5 years were recruited from four participating hospitals between June 2014 and July 2015. Each stool specimen was collected to detect a series of enteric pathogens, involving five viruses (Rotavirus group A, RVA; Norovirus, NoV; Sapovirus, SaV; Astrovirus, As; and Adenovirus, Ad), seven bacteria (diarrheagenic Escherichia coli, DEC; non-typhoidal Salmonella, NTS; Shigella spp...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27890800/metabolic-responses-of-haliotis-diversicolor-to-vibrio-parahaemolyticus-infection
#10
Jie Lu, Yanyan Shi, Shuhui Cai, Jianghua Feng
Vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone Haliotis diversicolor. Elucidation of metabolic mechanisms of abalones in responding to V. parahemolyticus infection is essential for controlling the epidemic. In this work, (1)H NMR-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of H. diversicolor after V...
November 25, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27890799/enhancing-gilthead-seabream-immune-status-and-protection-against-bacterial-challenge-by-means-of-antigens-derived-from-vibrio-parahaemolyticus
#11
Martha Reyes-Becerril, Crystal Guluarte, Diana Ceballos-Francisco, Carlos Angulo, M Ángeles Esteban
In an attempt to control the proliferation of the pathogenic bacterium Vibrio parahaemolyticus in gilthead seabream (Sparus aurata), the immunostimulant effect of lysate and ToxA from this bacterium was evaluated. Fish were intraperitoneally injected twice (first injection, day 1 of the experiment; second injection, day 7) and sampled after one week (on days 8 and 15). Afterwards, all fish specimens were experimentally infected with V. parahaemolyticus and mortality was recovered for 1 week. Fish injected with lysate, ToxA and phosphate buffer saline (control) showed 100%, 50% and 0% survival, respectively, when challenged with the pathogen...
November 25, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27888717/the-role-of-ficolin-like-protein-pcflp1-in-the-antibacterial-immunity-of-red-swamp-crayfish-procambarus-clarkii
#12
Yun-Jia Dai, Yu-Qing Wang, Ying-Hao Zhang, Yan Liu, Jin-Quan Li, Shun Wei, Li-Juan Zhao, Yong-Can Zhou, Li Lin, Jiang-Feng Lan
In invertebrates, ficolin-like proteins (FLPs) play important roles in innate immunity against pathogens. Previous studies primarily investigated the functions of FLPs in immune recognition, activation, and regulation. However, limited research has examined the functions of FLPs as immune effectors. In this work, a ficolin-like protein was identified in red swam crayfish (Procambarus clarkii) and designated as PcFLP1. Quantitative RT-PCR and western blot were employed to analyze the distribution and expression profiles of PcFLP1 in the tissues of the crayfish...
November 23, 2016: Molecular Immunology
https://www.readbyqxmd.com/read/27888131/hsp70-and-hsp90-are-involved-in-shrimp-penaeus-vannamei-tolerance-to-ahpnd-causing-strain-of-vibrio-parahaemolyticus-after-non-lethal-heat-shock
#13
Wisarut Junprung, Premruethai Supungul, Anchalee Tassanakajon
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in farmed penaeid shrimp throughout Asia. Previous studies reported that a non-lethal heat shock (NLHS) could enhance disease tolerance in aquatic animals. Here, we investigate whether the NLHS could enhance the survival of shrimp Penaeusvannamei upon challenge with an AHPND-causing strain of V. Parahaemolyticus (VPAHPND). Two NLHS conditions, acute and chronic NLHSs, were used...
November 22, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27886032/prevalence-and-factors-associated-with-different-pathogens-of-acute-diarrhea-in-adults-in-beijing-china
#14
Lei Jia, Changying Lin, Zhiyong Gao, Mei Qu, Junyong Yang, Jingyi Sun, He Chen, Quanyi Wang
INTRODUCTION: Only a small proportion of patients with diarrhea are diagnosed with laboratory tests in China, and most are diagnosed based on clinical symptoms. Therefore, understanding the prevalence of different diarrheal pathogens and their specific symptoms is important. METHODOLOGY: Data from a prospective study in Beijing of acute diarrhea and the related pathogens were used to study the association between different pathogen groups and the infected patients' characteristics...
November 24, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27871750/biogeochemical-and-hydrological-drivers-of-the-dynamics-of-vibrio-species-in-two-patagonian-estuaries
#15
Germán A Kopprio, M Eugenia Streitenberger, Kentaro Okuno, Mónica Baldini, Florencia Biancalana, Anna Fricke, Ana Martínez, Sucharit B Neogi, Boris P Koch, Shinji Yamasaki, Rubén J Lara
The ecology of the most relevant Vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in Argentinian Patagonia. Vibrio cholerae and Vibrio parahaemolyticus were reported in >29% of cases at the Río Colorado and Río Negro estuaries. Neither the pandemic serogroups of Vibrio cholerae O1, Vibrio cholerae O139 nor the cholera toxin gene were detected in this study. However, several strains of V. cholerae (not O1 or O139) are able to cause human disease or acquire pathogenic genes by horizontal transfer...
November 18, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27868074/cd63-promotes-hemocyte-mediated-phagocytosis-in-the-clam-paphia-undulata
#16
Mingjia Yu, Shanjun Yang, Hongxia Sun, Qiang Xia
As one of the surface membrane proteins of tetraspanin family, CD63 plays a crucial role in cellular trafficking and endocytosis, which also is associated with activation of a wide variety of immune cells. Here, the homolog of CD63 was characterized from one marine mollusk, Paphia undulata, which is designated as Pu-CD63. The complete cDNA of Pu-CD63 is 1,738 bp in length with an open reading frame (ORF) of 849 bp, encoding a 282 amino acid protein with four putative hydrophobic transmembrane helixes. Bioinformatic analysis revealed that Pu-CD63 contains one putative YXXØ consensus motif of "110-YVII-113" and one N-glycosylation site "155-NGT-157" within the large extracellular loop (LEL) region, supporting its conserved function in plasma membrane and endosomal/lysosomal trafficking...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/27854308/characterization-of-chemically-induced-bacterial-ghosts-bgs-using-sodium-hydroxide-induced-vibrio-parahaemolyticus-ghosts-vpgs
#17
Hyun Jung Park, Sung Oh, Nagarajan Vinod, Seongmi Ji, Han Byul Noh, Jung Mo Koo, Su Hyeong Lee, Sei Chang Kim, Ki-Sung Lee, Chang Won Choi
Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene E of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were generated by chemically-induced lysis and the method is based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH), acetic acid, boric acid, citric acid, maleic acid, hydrochloric acid, and sulfuric acid. The MIC values of the respective chemicals were 3.125, 6...
November 15, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27853457/differential-localization-of-chemotactic-signaling-arrays-during-the-lifecycle-of-vibrio-parahaemolyticus
#18
Jan Heering, Simon Ringgaard
When encountering new environments or changes to their external milieu, bacteria use elaborate mechanisms to respond accordingly. Here, we describe how Vibrio parahaemolyticus coordinates two such mechanisms - differentiation and chemotaxis. V. parahaemolyticus differentiates between two distinct cell types: short rod-shaped swimmer cells and highly elongated swarmer cells. We show that the intracellular organization of chemotactic signaling arrays changes according to the differentiation state. In swimmer cells chemotaxis arrays are strictly polarly localized, but in swarmer cells arrays form both at the cell poles and at irregular intervals along the entire cell length...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27843109/non-cholera-vibrios-the-microbial-barometer-of-climate-change
#19
REVIEW
Craig Baker-Austin, Joaquin Trinanes, Narjol Gonzalez-Escalona, Jaime Martinez-Urtaza
There is a growing interest in the role of climate change in driving the spread of waterborne infectious diseases, such as those caused by bacterial pathogens. One particular group of pathogenic bacteria - vibrios - are a globally important cause of diseases in humans and aquatic animals. These Gram-negative bacteria, including the species Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio cholerae, grow in warm, low-salinity waters, and their abundance in the natural environment mirrors ambient environmental temperatures...
November 11, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27836844/season-specific-occurrence-of-potentially-pathogenic-vibrio-spp-on-the-southern-coast-of-south-korea
#20
Doris Y W Di, Anna Lee, Jeonghwan Jang, Dukki Han, Hor-Gil Hur
: Vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. In this study, we aimed to isolate and observe the distribution of enteropathogenic Vibrio spp. from environments of the southern coast of South Korea over a season cycle. A total of 10,983 Vibrio spp. were obtained from tidal water and mud samples over a one-year period from five sampling sites along the southern west coast of South Korea...
November 11, 2016: Applied and Environmental Microbiology
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