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Vibrio parahaemolyticus

Ling-Ling Zhao, Kaimin Hui, Yue Wang, Hui Wang, Qian Ren, Xin-Cang Li
Galactoside-binding lectins, also known as galectins, play crucial roles in innate immune response in invertebrates. In this study, three cDNA sequences from Hyriopsis cumingii were identified and collectively called HcGalec genes. Each of the three deduced HcGalec proteins contained a galactose-binding lectin domain or a GLECT domain. All the three HcGalec genes are mainly present in the hepatopancreas and gills, and their expression is induced by bacterial challenge. Three recombinant HcGalec proteins can bind and agglutinate (Ca2+ -dependent) various microorganisms, including Gram-positive and Gram-negative bacteria...
February 20, 2018: Fish & Shellfish Immunology
Daniel Castillo, Diliana Pérez-Reytor, Nicolás Plaza, Sebastián Ramírez-Araya, Carlos J Blondel, Gino Corsini, Roberto Bastías, David E Loyola, Víctor Jaña, Leonardo Pavez, Katherine García
Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. As reported in other countries, after the rise and fall of the pandemic strain in Chile, other post-pandemic strains have been associated with clinical cases, including strains lacking the major toxins TDH and TRH. Since the presence or absence of tdh and trh genes has been used for diagnostic purposes and as a proxy of the virulence of V. parahaemolyticus isolates, the understanding of virulence in V. parahaemolyticus strains lacking toxins is essential to detect these strains present in water and marine products to avoid possible food-borne infection...
2018: Frontiers in Microbiology
P Carvajal-Rondanelli, M Aróstica, C A Álvarez, C Ojeda, F Albericio, L F Aguilar, S H Marshall, F Guzmán
Previous work demonstrated that lysine homopeptides adopt a polyproline II (PPII) structure. Lysine homopeptides with odd number of residues, especially with 11 residues (K11), were capable of inhibiting the growth of a broader spectrum of bacteria than those with an even number. Confocal studies also determined that K11 was able to localize exclusively in the bacterial membrane, leading to cell death. In this work, the mechanism of action of this peptide was further analyzed focused on examining the structural changes in bacterial membrane induced by K11, and in K11 itself when interacting with bacterial membrane lipids...
February 21, 2018: Amino Acids
Gao Xiaolong, Zhang Mo, Li Xian, Han Yin, Wu Fucun, Liu Ying
To study the effects of a probiotic (Bacillus lincheniformis) on the survival and growth of Haliotis discus hannai Ino, the expression levels of nonspecific immune genes and the resistance to Vibrio parahaemolyticus infection were assessed. Abalones (shell length: 27.64 ± 1.59 mm, body weight: 4.17 ± 0.32 g) were selected for use in an 8-week culture experiment and a 2-week V. parahaemolyticus artificial infection experiment. In both experiments, the control group (C) was fed with a basal feed and the experimental groups were fed with experimental food prepared by spraying the probiotic on the basal feed at different concentrations: 103 (B1), 105 (B2), and 107 (B3) cfu/mL...
February 17, 2018: Fish & Shellfish Immunology
Md Shamim Ahasan, Thomas B Waltzek, Roger Huerlimann, Ellen Ariel
Stranded green turtles (Chelonia mydas) are often cared for in rehabilitation centers until they recover. Although the specific causal agents of diseases in stranded turtles are difficult to diagnose, we know that gut microbiota of green turtles play a vital role in health as well as a wide range of diseases. The objective of this study was to characterize and compare the gut bacterial communities between pre-hospitalization (PH) and post-rehabilitation (PR) stranded green turtles using high-throughput sequencing analysis targeting V1-V3 regions of the bacterial 16S rRNA gene...
March 2018: Microbiological Research
Ben Niu, Lili Mu, Lili Xiao, Zhaohuan Zhang, Pradeep K Malakar, Haiquan Liu, Yingjie Pan, Yong Zhao
BACKGRROUD: Vibrio parahaemolyticus and Listeria monocytogenes are seafood pathogens of public health significance and predictive models are effective tools for quantitative microbial risk assessment (QMRA) of these pathogens. However, most current predictive models are based on growth of single strains in broth cultures and interactions of two or more bacteria in a food matrix can skew the outcomes of the predictions. Therefore, the impact of V. parahaemolyticus and L. monocytogenes when co-cultured and in mono culture on cooked shrimp at cold storage was investigated in this study...
February 19, 2018: Journal of the Science of Food and Agriculture
Jia-Jun Xie, Xu Chen, Tian-Yu Guo, Shi-Wei Xie, Hao-Hang Fang, Zhen-Lu Liu, Yan-Mei Zhang, Li-Xia Tian, Yong-Jian Liu, Jin Niu
Two trials were conducted to determine the effects of dietary Forsythia suspensa extract (FSE) on shrimp, Penaeus monodon, first on growth performance, second on the immune response and immune related gene expression of shrimp. In trial 1, shrimp (mean initial wet weight about 3.02 g) were fed with five diets containing 0% (basal diet), 0.01%, 0.02%, 0.04% and 0.06% FSE in triplicate for 60 days. Growth performance (final body wet weight, FBW; weight gain, WG; biomass gain, BG) of shrimp fed FSE diets were higher (P < 0...
February 15, 2018: Fish & Shellfish Immunology
Khwaja Salahuddin Siddiqi, Azamal Husen, Rifaqat A K Rao
Use of silver and silver salts is as old as human civilization but the fabrication of silver nanoparticles (Ag NPs) has only recently been recognized. They have been specifically used in agriculture and medicine as antibacterial, antifungal and antioxidants. It has been demonstrated that Ag NPs arrest the growth and multiplication of many bacteria such as Bacillus cereus, Staphylococcus aureus, Citrobacter koseri, Salmonella typhii, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Vibrio parahaemolyticus and fungus Candida albicans by binding Ag/Ag + with the biomolecules present in the microbial cells...
February 16, 2018: Journal of Nanobiotechnology
Ji-Young Ahn, Kyeong-Ah Lee, Moon-Jong Lee, Simranjeet Singh Sekhon, Sung-Keun Rhee, Sung-Jin Cho, Jung Ho Ko, Lyon Lee, Janet Han, Sang Yong Kim, Jiho Min, Yang-Hoon Kim
In this paper, whole-bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Vibrio parahaemolyticus. Round selection for V. parahaemolyticus was conducted 11 rounds, including two negative selection rounds. It was determined through real-time PCR amplification and post-SELEX experiment. The selected aptmers had high binding property and specificity to V. parahaemolyticus. Of 28 aptamers tested, VPCA-apta#1 had the highest binding affinity compared to other aptamer candidates obtained...
March 1, 2018: Journal of Nanoscience and Nanotechnology
Zhi-Xue Cheng, Xiao Chu, Sheng-Nan Wang, Xuan-Xian Peng, Hui Li
Polyvalent vaccines against more than one species of pathogens are especially important due to the complex ecosystem in aquaculture. We have previously shown that the development of polyvalent vaccines by shuffling six ompA genes from different bacteria with V. parahaemolyticus VP0764 primers. Here, we used the same 6 genes, V. alginolyticus VA0764 and VA1186, V. parahaemolyticus VP0764 and VP1186, E. tarda ompA and E. coli ompA, but with E. tarda ompA primers to develop new polyvalent vaccines. By this approach, we identified 7 potential polyvalent vaccines that were effective against both V...
February 10, 2018: Fish & Shellfish Immunology
Ying Li, Shuang Zhang, Mu He, Yanchun Zhang, Yanyan Fu, Hao Liang, Hongbo Jing, Yindong Li, Hongmei Ma, Maojun Zhang
Bacterial pathogens have been confirmed as the major cause of acute diarrhea among outpatients in China. In this study, 370 stool samples from the patients aged from 15 to 87 years old with diarrhea were collected over 12 months (from May 2016 to April 2017) in two hospitals in Shunyi, Beijing. Bacterial isolation was performed for the common enteric pathogens: Campylobacter, Salmonella, Shigella, Diarrheagenic Escherichia coli, and Vibrio parahaemolyticus for 370 samples. The filtration method was used for the Campylobacter isolation in this study...
2018: Frontiers in Microbiology
Jin Woo Jun, Jee Eun Han, Sib Sankar Giri, Kathy F J Tang, Xiaohui Zhou, Luis Fernando Aranguren, Hyoun Joong Kim, Saekil Yun, Cheng Chi, Sang Guen Kim, Se Chang Park
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has been one of the most problematic diseases in marine shrimp aquaculture throughout Southeast Asia and Latin America. To evaluate the effectiveness of a bacteriophage (phage) treatment for AHPND, a series of bioassays were carried out in a marine shrimp (Penaeus vannamei) model using an AHPND-V. parahaemolyticus strain that is highly pathogenic to shrimp. We monitored the mortality and histopathological changes during phage treatment...
March 2018: Indian Journal of Microbiology
Neha Rajpara, Mrinalini Nair, Ashima Kushwaha Bhardwaj
In an earlier study from this laboratory, Vibrio fluvialis BD146, a clinical isolate from Kolkata, India, 2002, was found to be resistant to all the fourteen antibiotics tested. It harboured a high copy number plasmid pBD146 and a low copy number plasmid. In the present study, a more detailed analysis was carried out to unravel different resistance mechanisms in this isolate. Sequencing showed that variable region of class 1 integron located on low copy number plasmid harbored arr3-cmlA-blaOXA10-aadA1 gene cassettes...
March 2018: Indian Journal of Microbiology
Liyuan Liu, Jinzhou Xiao, Mengmeng Zhang, Wanyu Zhu, Xiaoming Xia, Xilin Dai, Yingjie Pan, Shuling Yan, Yongjie Wang
The causative agent of shrimp AHPND was identified as specific Vibrio parahaemolyticus strains, which harbor a virulent plasmid that contains the toxic genes pirA and B (pirAB). Herein, a Vibrio bacterium was isolated from shrimp in Shanghai. This bacterium was identified as Vibrio owensii using 16S rRNA gene phylogeny, whole genome sequencing and comparative analysis. The V. owensii cells are rod-shape (1.86 ± 0.15 µm) with a single polar flagellum (4 µm). In addition, V. owensii form mauve colonies with jagged edges on CHROMagar plates...
February 7, 2018: Journal of Invertebrate Pathology
Lubna Ghenem, Nasreldin Elhadi
No abstract text is available yet for this article.
February 2018: Journal of Water and Health
Yuying Sun, Jiquan Zhang, Jianhai Xiang
Chitin deacetylase (CDA, EC, belonging to a family of extracellular chitin-modifying enzymes, can catalyze the deacetylation of chitin. In this study, the full-length cDNA sequence encoding chitin deacetylase 1 (EcCDA1) was obtained from the Exopalaemon carinicauda. The complete nucleotide sequence of EcCDA1 contained a 1611 bp open reading frame (ORF) encoding EcCDA1 precursor of 536 amino acids. The domain architecture of the deduced EcCDA1 protein contained a signal peptide, a chitin-binding peritrophin-A domain (ChtBD2), a low-density lipoprotein receptor class A domain (LDLa) and a Polysacc_deac_1 domain...
February 5, 2018: Fish & Shellfish Immunology
Xingning Xiao, Haiying Pang, Wen Wang, Weihuan Fang, Yingchun Fu, Yanbin Li
This study aimed to qualify the transfer of Vibrio parahaemolyticus during the shrimp peeling process via gloves under 3 different scenarios. The 1st 2 scenarios provided quantitative information for the probability distribution of bacterial transfer rates from (i) contaminated shrimp (6 log CFU/g) to non-contaminated gloves (Scenario 1) and (ii) contaminated gloves (6 log CFU/per pair) to non-contaminated shrimp (Scenario 2). In Scenario 3, bacterial transfer from contaminated shrimp to non-contaminated shrimp in the shrimp peeling process via gloves was investigated to develop a predictive model for describing the successive bacterial transfer...
February 7, 2018: Journal of Food Science
K Imaizumi, S Tinwongger, H Kondo, I Hirono
No abstract text is available yet for this article.
February 5, 2018: Journal of Fish Diseases
Bao Le, Seung Hwan Yang
Lactobacillus strains have been considered good candidates as biological control agents for prevention or treatment of plant and animal infections. One L. plantarum strain FB003 and three strains (FB011, FB081, and FB110) which closed to L. sakei were isolated from fermented and salted shrimp and their abilities in inhibiting growth of Vibrio parahaemolyticus were characterized. These strains were selected as potential probiotics based on their oro-gastro-intestinal resistance, gut colonization, adhesion to Caco-2 cells, antimicrobial activities, antibiotic resistance, and safety aspects...
February 2018: Journal of Microbiology / the Microbiological Society of Korea
Junhyeok Yu, Jeong-A Lim, Su-Jin Kwak, Jong-Hyun Park, Hyun-Joo Chang
Vibrio parahaemolyticus, a foodborne pathogen, has become resistant to antibiotics. Therefore, alternative bio-control agents such bacteriophage are urgently needed for its control. Six novel bacteriophages specific to V. parahaemolyticus (vB_VpaP_KF1~2, vB_VpaS_KF3~6) were characterized at the molecular level in this study. Genomic similarity analysis revealed that these six bacteriophages could be divided into two groups with different genomic features, phylogenetic grouping, and morphologies. Two groups of bacteriophages had their own genes with different mechanisms for infection, assembly, and metabolism...
February 1, 2018: Archives of Virology
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