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Vibrio parahaemolyticus

George Osei-Adjei, He Gao, Ying Zhang, Lingyu Zhang, Wenhui Yang, Huiying Yang, Zhe Yin, Xinxiang Huang, Yiquan Zhang, Dongsheng Zhou
Vibrio parahaemolyticus is the leading cause of seafood-associated gastroenteritis. Type III secretion system 1 (T3SS1) is one of the virulence determinants of this bacteria. T3SS1 expression is regulated by ToxR and CalR. ToxR represses the transcription of T3SS1 genes via activation of CalR, which acts as a transcriptional repressor of T3SS1 genes. However, the transcriptional regulation mechanisms have not been elucidated. As showing in the present work, ToxR binds to the promoter DNA region of calR to activate its transcription...
September 12, 2017: Oncotarget
Nor Azman Kasan, Nurarina Ayuni Ghazali, Mhd Ikhwanuddin, Zaharah Ibrahim
BACKGROUND AND OBJECTIVE: A new green technology to reduce environmental damages while optimizing production of Pacific Whiteleg shrimp, Litopenaeus vannamei was developed known as "Biofloc technology". Microbial communities in biofloc aggregates are responsible in eliminating water exchange and producing microbial proteins that can be used as supplemented feed for L. vannamei. This study aimed to isolate and identify potential bioflocculant-producing bacteria to be used as inoculum for rapid formation of biofloc...
2017: Pakistan Journal of Biological Sciences: PJBS
Jake D Thickman, Christopher J Gobler
Food safety is a major concern in the shellfish industry, as severe illness can result from consuming shellfish that have accumulated waterborne pathogens. Shellfish harvesting areas are typically monitored for indicator bacteria such as fecal coliforms that serve as proxies for enteric pathogens although these indicators have shown little relation to some naturally occurring pathogenic bacteria such as Vibrio parahaemolyticus. To examine the dynamics and ecology of pathogenic and non-pathogenic strains of V...
2017: PloS One
Peng-Hsun Chase Chen, Sheng-Yang Ho, Pin-Lung Chen, Tzu-Chiao Hung, An-Jou Liang, Tang-Feng Kuo, Hsiao-Chun Huang, Tsung-Shing Andrew Wang
Siderophores are small molecules used to specifically transport iron into bacteria via related receptors. By adapting siderophores and hijacking their pathways, we may discover an efficient and selective way to target microbes. Herein, we report the synthesis of a siderophore-fluorophore conjugate VF-FL derived from vibrioferrin (VF). Using flow cytometry and fluorescence microscopy, the probe selectively labeled vibrios, including V. parahaemolyticus, V. cholerae, and V. vulnificus, even in the presence of other species such as S...
October 16, 2017: ACS Chemical Biology
Carlos Angulo, Erika Alamillo, Felipe Ascencio, Martha Reyes-Becerril
The nuclear factor of activated T cells (NFAT) proteins have crucial roles in the development and function of the immune system since they not only regulate activation of T cells but are also involved in the control of thymocyte development and T-cell differentiation. In this study, NFATc3 was characterized from the Pacific red snapper, Lutjanus peru. LpNFAtc3, which contains an open reading of 3300 bp frame coding for a protein of 1100 aa with a predicted molecular weight of 118.52 kDa. The predicted protein showed a conserved NFAT family structure with signature motifs and domains, sharing high identity (up to 76%) compared to other fish sequences...
October 3, 2017: Developmental and Comparative Immunology
Diliana Pérez-Reytor, Katherine García
No abstract text is available yet for this article.
October 5, 2017: Virulence
Lakkakula Satish, Sivasubramanian Santhakumari, Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian, Arumugam Veera Ravi, Manikandan Ramesh
The present study explores the non-bactericidal anti-virulence efficacy of green synthesized silver nanoparticles (AgNPs) from Gelidiella acerosa against multi-drug resistant Vibrio spp. Spectral characterization of AgNPs was performed through UV-Visible, FT-IR, and energy-dispersive spectroscopic techniques followed by X-ray crystallography and zeta potential analysis. Further, the structural characterization was done by electron and atomic force microscopic techniques. AgNPs profoundly quelled the quorum sensing mediated violacein production in Chromobacterium violaceum and CV026...
September 30, 2017: Environmental Science and Pollution Research International
Xiaoze Xie, Mengnan Chen, Aiyi Zhu
Glutathione peroxidases, a vital family of antioxidant enzymes in oxybiotic organisms, are involved in anti-pathogen immune response. In this study, two complete selenium-dependent glutathione peroxidase 1 cDNAs (designated as LcGPx1a and LcGPx1b) were obtained from the large yellow croaker Larimichthys crocea by rapid amplification of cDNA ends. The full-length sequence of LcGPx1a was 917 bp with a 5'-untranslated region (UTR) of 52 bp, a 3'-UTR of 289 bp, and an open reading frame of 576 bp encoding 191 amino acid (aa) polypeptides...
September 27, 2017: Fish & Shellfish Immunology
Yuying Sun, Jiquan Zhang, Jianhai Xiang
Chitinase, belonging to family 18 glycosyl hydrolase, is a multi-gene family and it has many functions. Generation of loss-of-function mutant targeting an interesting gene is a common way to clarify its function based on reverse genetics. In this study, we first reported the immune defense of a chitinase gene (EcChi4) in Exopalaemon carinicauda using its EcChi4-deletion mutant. EcChi4 was predominantly expressed in hepatopancreas and was upregulated after challenge with Vibrio parahaemolyticus or Aeromonas hydrophila...
September 28, 2017: Fish & Shellfish Immunology
Sariqa Wagley, Richard Borne, Jamie Harrison, Craig Baker-Austin, Donatella Ottaviani, Francesca Leoni, Varaporn Vuddhakul, Richard W Titball
Non-toxigenic V. parahaemolyticus isolates (tdh(-)/trh(-)/T3SS2(-)) have recently been isolated from patients with gastroenteritis. In this study we report that the larvae of the wax moth (Galleria mellonella) are susceptible to infection by toxigenic or non-toxigenic clinical isolates of V. parahaemolyticus. In comparison larvae inoculated with environmental isolates of V. parahaemolyticus did not succumb to disease. Whole genome sequencing of clinical non-toxigenic isolates revealed the presence of a gene encoding a nudix hydrolase, identified as mutT...
September 29, 2017: Virulence
Maryse Bonnin-Jusserand, Stéphanie Copin, Cédric Le Bris, Thomas Brauge, Mélanie Gay, Anne Brisabois, Thierry Grard, Graziella Midelet-Bourdin
Seafood products are widely consumed all around the world and play a significant role on the economic market. Bacteria of the Vibrio genus can contaminate seafood and thus pose a risk to human health. Three main Vibrio species, V. cholerae, V. parahaemolyticus and V. vulnificus, are potentially pathogenic to humans. These species are responsible for a dramatic increase of seafood-borne infections, worldwide. Hence, early detection of total and pathogenic Vibrio is needed and should rely on quick and effective methods...
September 28, 2017: Critical Reviews in Food Science and Nutrition
Justine Fri, Roland Ndip Ndip, Henry Akum Njom, Anna Maria Clarke
Background: Seafood-borne Vibrio infections, often linked to contaminated seafood and water, are of increasing global public health concern. The aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and Kareiga estuary, South Africa. Methods: A total of 200 samples including dusky kob fish (n = 120) and seawater (n = 80) were subjected to Vibrio screening on thiosulfate-citrate-bile salts-sucrose agar (TCBS)...
September 25, 2017: International Journal of Environmental Research and Public Health
Behnam Mahdavi, Wan A Yaacob, Laily B Din
OBJECTIVE: To report the chemical composition and bioactivity (including antioxidant and antimicrobial activity) of essential oils from the rhizomes, stems, and leaves of Etlingera sayapensis (E. sayapensis) A.D. Poulsen & Ibrahim for the first time. METHODS: First, the essential oils were obtained using a Clevenger-type apparatus. Then, the essential oils compositions were identified by chromatography methods including GC-FID and GC-MS. For the next step, DPPH radical scavenging activity (RSA), β-carotene bleaching (BCB), and ferrous ion chelating ability (FIC) were chosen to evaluate the essential oils antioxidant activity...
August 2017: Asian Pacific Journal of Tropical Medicine
Yuting Li, Tao Zhang, Xin Zhang, Guodong Wang, Yilei Wang, Ziping Zhang
Heat shock cognate 70 (HSC70) is a class of highly conserved proteins which functions as a molecular chaperon, participates in tolerance processes, and is involved in protein folding, degradation, targeting, translocation, and protein complex remodeling. In this study, the mRNA expression level of the Haliotis diversicolor HSC70 (HdHSC70) gene was detected by quantitative real-time PCR in different tissues and under different stresses. The results showed that the HdHSC70 gene was ubiquitously expressed in seven selected tissues...
September 23, 2017: Cell Stress & Chaperones
Sheng Wang, Bin Yin, Haoyang Li, Bang Xiao, Kai Lǚ, Chiguang Feng, Jianguo He, Chaozheng Li
LvMKK4, a homologue of the mammalian mitogen-activated protein kinase kinase 4 (MKK4), was isolated and identified from Litopenaeus vannamei in the present study. The full-length cDNA of LvMKK4 is 1947 bp long, with an open reading frame (ORF) of 1185 bp encoding a putative protein of 388 amino acids. LvMKK4 contains several characteristic domains such as D domain, SIAKT motif and kinase domain, all of which are conserved in MAP kinase kinase family. Like mammalian MKK4 but not Drosophila MKK4, LvMKK4 could bind to, phosphorylate and activate p38 MAPK, which provided some insights into the signal transduction mechanism of MKK4-p38 cascade in invertebrates...
September 19, 2017: Developmental and Comparative Immunology
Yao-Meng Wu, Lei Yang, Xue-Jie Li, Lu Li, Qun Wang, Wei-Wei Li
Scavenger receptors (SRs) are important pattern recognition receptors (PRRs), which play significant roles in host defense against pathogens by identifying pathogen-associated molecular patterns (PAMPs). In this study, we report the cloning and characterization of a SR from Eriocheir sinensis (EsSR-B1) which is a 500 amino acid protein encoded by a gene comprised of 2726 nucleotides with a 1503 bp open reading frame. The domains of EsSR-B1 were found to be evolutionarily conserved. EsSR-B1 was widely detected in different tissues of E...
September 13, 2017: Fish & Shellfish Immunology
Huamin Zhong, Yukui Zhong, Qiulian Deng, Zhenwen Zhou, Xiaoshan Guan, Muxia Yan, Tingting Hu, Mingyong Luo
In the Viable but Non-Culturable (VBNC) state, microorganisms may survive under severe external environment. In this study, the specificity and sensitivity of PMA-LAMP assay on the detection of Vibrio Parahemolyticus (V. parahemolyticus) has been developed and evaluated, with further application on a number of food-borne V. parahemolyticus strains. Six primers were designed for recognizing 8 distinct targeting on tlh, tdh and trh gene. Through specific penetration through the damaged cell membrane of dead cells and intercalating into DNA, PMA could prevent DNA amplification of dead bacteria from LAMP, which enabled the differentiation of bacteria between VBNC state and dead state...
October 2017: Microbial Pathogenesis
Y Xing, C Jiang, W Y Hua, F Liu, Z Zhao, Y J Ding, L Wang, J Li
Objective: To study the epidemiological characteristics of an outbreak caused by norovirus infection in a school in Haidian district, Beijing. Methods: Basic information of the school and data related to patients in the fields survey were collected and analyzed descriptively. Laboratory tests were performed to test the stool and anal swab specimens of both patients and cooks as well as the environmental specimens. Risk factors related to the incidence were analyzed through a case-control study. Results: A total number of 119 patients were identified in the school...
September 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Cancan Qi, Lei Wang, Mei Liu, Keyong Jiang, Mengqiang Wang, Wei Zhao, Baojie Wang
The white shrimp Litopenaeus vannamei has been greatly impacted by Vibrio infection. In this study, we investigated the intestinal barrier response of L vannamei following challenge with Vibrio parahaemolyticus E1, by examining morphological changes and transcriptome expression levels. A total of 16,4420 unigenes were obtained from RNAseq data after quality control and assembly, and 4646 differentially expressed genes (DEGs) were identified following Vibrio challenge, of which 2469 unigenes were significantly up-regulated and 2177 were significantly down-regulated...
September 6, 2017: Fish & Shellfish Immunology
Yanmei Li, Ling Yang, Jie Fu, Muxia Yan, Dingqiang Chen, Li Zhang
As a self-protection mechanism, the viable but non-culturable (VBNC) state provides the ability against conventional detection methods among various foodborne pathogens. The ability of forming colonies is lost while metabolism is still maintaining in VBNC state cells. Recently, ethidium monoazide (EMA) and propidium monoazide (PMA) have been widely applied on the detection of foodborne pathogens in VBNC state. Combined with loop-mediated isothermal amplification (LAMP), the PMA/EMA-LAMP showed a significant priority in high sensitivity, specificity and rapidity over conventional PCR based assays...
October 2017: Microbial Pathogenesis
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