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Marica Bakovic

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https://www.readbyqxmd.com/read/27188900/oat-%C3%AE-glucan-depresses-sglt1-and-glut2-mediated-glucose-transport-in-intestinal-epithelial-cells-iec-6
#1
Nazanin N Abbasi, Peter P Purslow, Susan M Tosh, Marica Bakovic
Oat β-glucan consumption is linked to reduced risk factors associated with diabetes and obesity by lowering glycemic response and serum level of low-density lipoproteins. The purpose of this study was to identify the mechanism of action of oat β-glucan at the interface between the gut wall and the lumen responsible for attenuating glucose levels. We proposed that viscous oat β-glucan acts as a physical barrier to glucose uptake in normally absorptive gut epithelial cells IEC-6 by affecting the expression of intestinal glucose transporters...
June 2016: Nutrition Research
https://www.readbyqxmd.com/read/26242921/choline-supplementation-restores-substrate-balance-and-alleviates-complications-of-pcyt2-deficiency
#2
Laila C Schenkel, Sugashan Sivanesan, Junzeng Zhang, Birgitte Wuyts, Adrian Taylor, Adronie Verbrugghe, Marica Bakovic
Choline plays a critical role in systemic lipid metabolism and hepatic function. Here we conducted a series of experiments to investigate the effect of choline supplementation on metabolically altered Pcyt2(+/-) mice. In Pcyt2(+/-) mice, the membrane phosphatidylethanolamine (PE) turnover is reduced and the formation of fatty acids (FA) and triglycerides (TAG) increased, resulting in hypertriglyceridemia, liver steatosis and obesity. One month of choline supplementation reduced the incorporation of FA into TAG and facilitated TAG degradation in Pcyt2(+/-) adipocytes, plasma and liver...
November 2015: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/25986609/male-specific-cardiac-dysfunction-in-ctp-phosphoethanolamine-cytidylyltransferase-pcyt2-deficient-mice
#3
Poulami Basu, Faisal J Alibhai, Elena V Tsimakouridze, Ratnesh K Singh, Sabina Paglialunga, Graham P Holloway, Tami A Martino, Marica Bakovic
Phosphatidylethanolamine (PE) is the most abundant inner membrane phospholipid. PE synthesis from ethanolamine and diacylglycerol is regulated primarily by CTP:phosphoethanolamine cytidylyltransferase (Pcyt2). Pcyt2(+/-) mice have reduced PE synthesis and, as a consequence, perturbed glucose and fatty acid metabolism, which gradually leads to the development of hyperlipidemia, obesity, and insulin resistance. Glucose and fatty acid uptake and the corresponding transporters Glut4 and Cd36 are similarly impaired in male and female Pcyt2(+/-) hearts...
August 2015: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/25826667/translation-attenuation-via-3-terminal-codon-usage-in-bovine-csn1s2-is-responsible-for-the-difference-in-%C3%AE-s2-and-%C3%AE-casein-profile-in-milk
#4
Julie J Kim, Jaeju Yu, Jnanankur Bag, Marica Bakovic, John P Cant
The rate of secretion of αs2-casein into bovine milk is approximately 25% of that of β-casein, yet mammary expression of their respective mRNA transcripts (csn1s2 and csn2) is not different. Our objective was to identify molecular mechanisms that explain the difference in translation efficiency between csn1s2 and csn2. Cell-free translational efficiency of csn2 was 5 times that of csn1s2. Transcripts of csn1s2 distributed into heavier polysomes than csn2 transcripts, indicating an attenuation of elongation and/or termination...
2015: RNA Biology
https://www.readbyqxmd.com/read/25466896/mechanism-of-choline-deficiency-and-membrane-alteration-in-postural-orthostatic-tachycardia-syndrome-primary-skin-fibroblasts
#5
COMPARATIVE STUDY
Laila C Schenkel, Ratnesh K Singh, Vera Michel, Steven H Zeisel, Kerry-Ann da Costa, Amy R Johnson, Harvey S Mudd, Marica Bakovic
Fibroblasts from a patient with postural orthostatic tachycardia syndrome (POTS), who presented with low plasma choline and betaine, were studied to determine the metabolic characteristics of the choline deficiency. Choline is required for the synthesis of the phospholipid phosphatidylcholine (PC) and for betaine, an important osmoregulator. Here, choline transport, lipid homeostasis, and mitochondria function were analyzed in skin fibroblasts from POTS and compared with control cells. The choline transporter-like protein 1/solute carrier 44A1 (CTL1/SLC44A1) and mRNA expression were 2-3 times lower in POTS fibroblasts, and choline uptake was reduced 60% (P < 0...
May 2015: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/25244368/dietary-regulation-of-keap1-nrf2-are-pathway-focus-on-plant-derived-compounds-and-trace-minerals
#6
REVIEW
Amanda L Stefanson, Marica Bakovic
It has become increasingly evident that chronic inflammation underpins the development of many chronic diseases including cancer, cardiovascular disease and type 2 diabetes. Oxidative stress is inherently a biochemical dysregulation of the redox status of the intracellular environment, which under homeostatic conditions is a reducing environment, whereas inflammation is the biological response to oxidative stress in that the cell initiates the production of proteins, enzymes, and other compounds to restore homeostasis...
September 19, 2014: Nutrients
https://www.readbyqxmd.com/read/24972900/palmitic-acid-and-oleic-acid-differentially-regulate-choline-transporter-like-1-levels-and-glycerolipid-metabolism-in-skeletal-muscle-cells
#7
Laila Cigana Schenkel, Marica Bakovic
Choline is an essential nutrient required for the biosynthesis of membrane lipid phosphatidylcholine (PtdCho). Here we elucidate the mechanism of how palmitic acid (PAM) and oleic acid (OLA) regulate choline transporter-like protein 1 (CTL1/SLC44A1) function. We evaluated the mechanism of extracellular and intracellular transport of choline, and their contribution to PtdCho and other glycerolipid-diacylglycerol (DAG) and triacylglycerol (TAG) homeostasis in differentiated skeletal muscle cells. PAM reduces total and plasma membrane CTL1/SLC44A1 protein by lysosomal degradation, and limits the choline uptake while increasing DAG and TAG synthesis...
August 2014: Lipids
https://www.readbyqxmd.com/read/24703999/a-novel-murine-ctp-phosphoethanolamine-cytidylyltransferase-splice-variant-is-a-post-translational-repressor-and-an-indicator-that-both-cytidylyltransferase-domains-are-required-for-activity
#8
Zvezdan Pavlovic, Ratnesh Kumar Singh, Marica Bakovic
CTP:phosphoethanolamine cytidylyltransferase (Pcyt2) has an important regulatory function in biosynthesis of the membrane phospholipid phosphatidylethanolamine. We previously determined that the full-length Pcyt2α and its splice variant Pcyt2β are the main active isoforms of this enzyme. Here we report that mouse Pcyt2 could be spliced at Introns 7 and 8 to produce a unique third isoform, Pcyt2γ, in which the second cytidylyltransferase domain at the C-terminus becomes deleted. Pcyt2γ is ubiquitously expressed in embryonic and adult mouse tissues, and is the most abundant in the kidney, skeletal muscle and testis...
June 10, 2014: Gene
https://www.readbyqxmd.com/read/24578708/formation-and-regulation-of-mitochondrial-membranes
#9
REVIEW
Laila Cigana Schenkel, Marica Bakovic
Mitochondrial membrane phospholipids are essential for the mitochondrial architecture, the activity of respiratory proteins, and the transport of proteins into the mitochondria. The accumulation of phospholipids within mitochondria depends on a coordinate synthesis, degradation, and trafficking of phospholipids between the endoplasmic reticulum (ER) and mitochondria as well as intramitochondrial lipid trafficking. Several studies highlight the contribution of dietary fatty acids to the remodeling of phospholipids and mitochondrial membrane homeostasis...
2014: International Journal of Cell Biology
https://www.readbyqxmd.com/read/24519946/isoform-specific-and-protein-kinase-c-mediated-regulation-of-ctp-phosphoethanolamine-cytidylyltransferase-phosphorylation
#10
Zvezdan Pavlovic, Lin Zhu, Leanne Pereira, Ratnesh Kumar Singh, Rosemary B Cornell, Marica Bakovic
CTP:phosphoethanolamine cytidylyltransferase (Pcyt2) is the main regulatory enzyme for de novo biosynthesis of phosphatidylethanolamine by the CDP-ethanolamine pathway. There are two isoforms of Pcyt2, -α and -β; however, very little is known about their specific roles in this important metabolic pathway. We previously demonstrated increased phosphatidylethanolamine biosynthesis subsequent to elevated activity and phosphorylation of Pcyt2α and -β in MCF-7 breast cancer cells grown under conditions of serum deficiency...
March 28, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24142790/meclizine-inhibits-mitochondrial-respiration-through-direct-targeting-of-cytosolic-phosphoethanolamine-metabolism
#11
Vishal M Gohil, Lin Zhu, Charli D Baker, Valentin Cracan, Abbas Yaseen, Mohit Jain, Clary B Clish, Paul S Brookes, Marica Bakovic, Vamsi K Mootha
We recently identified meclizine, an over-the-counter drug, as an inhibitor of mitochondrial respiration. Curiously, meclizine blunted respiration in intact cells but not in isolated mitochondria, suggesting an unorthodox mechanism. Using a metabolic profiling approach, we now show that treatment with meclizine leads to a sharp elevation of cellular phosphoethanolamine, an intermediate in the ethanolamine branch of the Kennedy pathway of phosphatidylethanolamine biosynthesis. Metabolic labeling and in vitro enzyme assays confirmed direct inhibition of the cytosolic enzyme CTP:phosphoethanolamine cytidylyltransferase (PCYT2)...
December 6, 2013: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/23877091/peculiarities-of-one-carbon-metabolism-in-the-strict-carnivorous-cat-and-the-role-in-feline-hepatic-lipidosis
#12
REVIEW
Adronie Verbrugghe, Marica Bakovic
Research in various species has indicated that diets deficient in labile methyl groups (methionine, choline, betaine, folate) produce fatty liver and links to steatosis and metabolic syndrome, but also provides evidence of the importance of labile methyl group balance to maintain normal liver function. Cats, being obligate carnivores, rely on nutrients in animal tissues and have, due to evolutionary pressure, developed several physiological and metabolic adaptations, including a number of peculiarities in protein and fat metabolism...
July 2013: Nutrients
https://www.readbyqxmd.com/read/23354482/regulation-of-phosphatidylethanolamine-homeostasis-8212-the-critical-role-of-ctp-phosphoethanolamine-cytidylyltransferase-pcyt2
#13
Zvezdan Pavlovic, Marica Bakovic
Phosphatidylethanolamine (PE) is the most abundant lipid on the protoplasmatic leaflet of cellular membranes. It has a pivotal role in cellular processes such as membrane fusion, cell cycle regulation, autophagy, and apoptosis. CTP:phosphoethanolamine cytidylyltransferase (Pcyt2) is the main regulatory enzyme in de novo biosynthesis of PE from ethanolamine and diacylglycerol by the CDP-ethanolamine Kennedy pathway. The following is a summary of the current state of knowledge on Pcyt2 and how splicing and isoform specific differences could lead to variations in functional properties in this family of enzymes...
2013: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/22845000/editorial-choline-and-brain-function
#14
EDITORIAL
Vera Michel, Marica Bakovic
No abstract text is available yet for this article.
June 2012: Central Nervous System Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/22820672/liver-x-receptor-agonists-inhibit-the-phospholipid-regulatory-gene-ctp-phosphoethanolamine-cytidylyltransferase-pcyt2
#15
Lin Zhu, Marica Bakovic
Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2). In the mouse embryonic fibroblasts C3H10T1/2, the LXR synthetic agonist TO901317 lowered Pcyt2 promoter-luciferase activity in a concentration-dependent manner. Furthermore, 25-OH and TO901317 reduced mouse Pcyt2 mRNA and protein levels by 35-60%...
2008: Research Letters in Biochemistry
https://www.readbyqxmd.com/read/22764088/mechanism-of-hypertriglyceridemia-in-ctp-phosphoethanolamine-cytidylyltransferase-deficient-mice
#16
Ratnesh Kumar Singh, Morgan D Fullerton, Donna Vine, Marica Bakovic
Phosphatidylethanolamine is an important inner-leaflet phospholipid, and CTP:phosphoethanolamine cytidylyltransferase-Pcyt2 acts as the main regulator of the de novo phosphatidylethanolamine synthesis from ethanolamine and diacylglycerol. Complete deletion of the mouse Pcyt2 gene is embryonic lethal, and the single-allele deficiency leads to development of the metabolic syndrome phenotype, including liver steatosis, hypertriglyceridemia, obesity, and insulin resistance. This study aimed to specifically elucidate the mechanisms of hypertriglyceridemia in Pcyt2 heterozygous mice (Pcyt2(+/-))...
September 2012: Journal of Lipid Research
https://www.readbyqxmd.com/read/22483272/the-ubiquitous-choline-transporter-slc44a1
#17
REVIEW
Vera Michel, Marica Bakovic
Choline is a zwitter ion that is positively charged at certain pH, which necessitates transport systems to allow this amine to cross the phospholipid bilayer of cellular membranes. The solute carrier 44A1 (SLC44A1), also referred to as choline transporter-like protein 1 (CTL1), is a recently discovered choline transporter with an intermediate affinity for choline; this transport is Na(+)-independent and sensitive to inhibition by the drug hemicholinium-3. We highlight in this review the discovery and characterization of SLC44A1, describe its expression patterns and subcellular localization, and summarize evidence for the role of this choline transporter in the central nervous system...
June 2012: Central Nervous System Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/22339418/breast-cancer-cells-adapt-to-metabolic-stress-by-increasing-ethanolamine-phospholipid-synthesis-and-ctp-ethanolaminephosphate-cytidylyltransferase-pcyt2-activity
#18
Lin Zhu, Marica Bakovic
The significance of phosphatidylethanolamine (PE) in breast cancer cell metabolism was investigated under stress conditions caused by serum deficiency. Serum deficient MCF-7 cells adapt to stress conditions by increasing synthesis and content of PE and diacylglycerol (DAG). The biosynthesis of PE from DAG and ethanolamine was regulated at the level of formation of CDP-ethanolamine, the metabolic step catalyzed by Pcyt2. The catalytic activity of Pcyt2 was elevated 2-3-fold, yet the enzyme remained rate-limiting in serum-deficient cells...
April 2012: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/22291708/forms-crosstalks-and-the-role-of-phospholipid-biosynthesis-in-autophagy
#19
Leanne Pereira, John Paul Girardi, Marica Bakovic
Autophagy is a highly conserved cellular process occurring during periods of stress to ensure a cell's survival by recycling cytosolic constituents and making products that can be used in energy generation and other essential processes. Three major forms of autophagy exist according to the specific mechanism through which cytoplasmic material is transported to a lysosome. Chaperone-mediated autophagy is a highly selective form of autophagy that delivers specific proteins for lysosomal degradation. Microautophagy is a less selective form of autophagy that occurs through lysosomal membrane invaginations, forming tubes and directly engulfing cytoplasm...
2012: International Journal of Cell Biology
https://www.readbyqxmd.com/read/21773586/the-impact-of-choline-availability-on-muscle-lipid-metabolism
#20
Vera Michel, Ratnesh Kumar Singh, Marica Bakovic
Consumption of choline-rich foods is essential to ensure membrane integrity, neurotransmission and genomic methylation pathways. Insufficient dietary choline supply can cause choline deficiency (CD) which manifests in the development of non-alcoholic fatty liver disease. There is very limited information regarding the effect of CD on non-hepatic tissues such as muscle. In this study, we induced CD in muscle cells and investigated the effect on choline transport, phosphatidylcholine (PC), fatty acid and triacylglycerol (TAG, fat) metabolism...
January 2011: Food & Function
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