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https://www.readbyqxmd.com/read/28207310/microbiological-quality-and-prevalence-of-%C3%AE-lactam-antibiotic-resistance-genes-in-oysters-crassostrea-rhizophorae
#1
Maria Aparecida da RessurreiÇão Brandão, Amanda Teixeira Sampaio Lopes, Maria Tereza da Silva Neta, Rhyan Barros Farias de Oliveira, Rachel Passos Rezende, George Rêgo Albuquerque, Verônica Dias Gonçalves, Dália Dos Prazeres Rodrigues, Guisla Boehs, Bianca Mendes Maciel
The microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. The objective of this study was to assess the bacterial composition of the edible oyster Crassostrea rhizophorae in urban and preserved estuaries. Particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blaTEM, blaSHV, and blaKPC) via quantitative PCR of oyster DNA...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28204862/effect-of-constant-predictable-and-unpredictable-motor-tasks-on-motor-performance-and-blood-markers-of-stress
#2
Laura Kyguoliene, Albertas Skurvydas, Nerijus Eimantas, Neringa Baranauskienė, Dalia Mickeviciene, Daiva Urboniene, Margarita Cernych, Marius Brazaitis
An unfamiliar or novel physical stimulus induces activation of dopaminergic neurons within the brain and greater activity in areas involved in emotion; considering this, we aimed to establish whether unpredictable prolonged (fatiguing) motor task (vs. constant vs. predictable) evokes greater dopaminergic activity, enhances neuromuscular performance, motor accuracy, and perception of effort, and delays overall central fatigue. Fifteen healthy male volunteers (aged 22 ± 4 years) were required to perform 1 of 3 exercise trials (at least 1 week apart) of 100 intermittent isometric contraction (IIC) tasks involving knee extensions at 60° flexion...
February 16, 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/28196401/systematic-genetic-dissection-of-pts-in-vibrio-cholerae-uncovers-a-novel-glucose-transporter-and-a-limited-role-for-pts-during-infection-of-a-mammalian-host
#3
Chelsea A Hayes, Triana N Dalia, Ankur B Dalia
A common mechanism for high affinity carbohydrate uptake in microbial species is the phosphoenolpyruvate-dependent phosphotransferase system (PTS). This system consists of a shared component, EI, which is required for all PTS transport, and numerous carbohydrate uptake transporters. In V. cholerae, there are 13 distinct PTS transporters. Due to genetic redundancy within this system, the carbohydrate specificity of each of these transporters is not currently defined. Here, using multiplex genome editing by natural transformation (MuGENT), we systematically dissect PTS transport in V...
February 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28194056/prevalence-and-prognostic-value-of-idh1-r132-mutation-in-newly-diagnosed-aml-egyptian-patients-with-normal-karyotype
#4
Dalia Salem, Sherin Abd El-Aziz, Nadia El-Menshawy, Tarek Abouzeid, Mohamed Ebrahim
Mutation in IDH1 gene was suggested to be associated with bad prognosis in cytogenetically normal AML (CN-AML). However, there are conflicting data about its prognostic impact. Besides, its prevalence and prognostic significance in Egyptian patients still not fully stated. We aimed to assess the prevalence of IDH1(R132) mutation in Egyptian CN-AML patients, its correlation with FAB subtypes, and clinical outcome of those patients. Sequencing of amplified IDH1 gene exon four from 50 patients was performed to detect codon R132 point mutation...
March 2017: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/28194042/does-mmp-9-gene-polymorphism-play-a-role-in-pituitary-adenoma-development
#5
Brigita Glebauskiene, Rasa Liutkeviciene, Alvita Vilkeviciute, Loresa Kriauciuniene, Silvija Jakstiene, Egle Zlatkute, Abdonas Tamosiunas, Reda Zemaitiene, Paulina Vaitkiene, Dalia Zaliuniene
Purpose. To determine if the MMP-9 genotype has an influence on development of pituitary adenoma (PA). Methodology. The study enrolled n = 86 patients with PA and n = 526 healthy controls (reference group). The genotyping of MMP-9 was carried out using the real-time polymerase chain reaction method. Results. Our data demonstrated that the MMP-9 (-1562) C/C genotype was more frequent in PA group than in healthy controls (81.4% versus 64.6%, p = 0.002); C/C genotype was more frequently present in PA females compared to healthy control females, 81...
2017: Disease Markers
https://www.readbyqxmd.com/read/28192446/range-extension-for-the-common-dolphin-delphinus-sp-to-the-colombian-caribbean-with-taxonomic-implications-from-genetic-barcoding-and-phylogenetic-analyses
#6
Nohelia Farías-Curtidor, Dalia C Barragán-Barrera, Paula Alejandra Chávez-Carreño, Cristina Jiménez-Pinedo, Daniel M Palacios, Dalila Caicedo, Fernando Trujillo, Susana Caballero
The nearest known population of common dolphins (Delphinus sp.) to the Colombian Caribbean occurs in a fairly restricted range in eastern Venezuela. These dolphins have not been previously reported in the Colombian Caribbean, likely because of a lack of study of the local cetacean fauna. We collected cetacean observations in waters of the Guajira Department, northern Colombia (~11°N, 73°W) during two separate efforts: (a) a seismic vessel survey (December 2009-March 2010), and (b) three coastal surveys from small boats (May-July 2012, May 2013, and May 2014)...
2017: PloS One
https://www.readbyqxmd.com/read/28185016/association-between-paraoxonase-1-gene-q192r-and-l55m-polymorphisms-in-systemic-lupus-erythematosus-sle-and-anti-phospholipid-syndrome-aps-in-a-population-from-cairo-of-egypt
#7
Alshaymaa Ahmed Ibrahim, Dalia El-Lebedy, Ingy Ashmawy, Maha Abdel Hady
Paraoxonase-1 (PON1) is involved in the oxidative stress process that cause tissue damage observed in systemic lupus erythematosus (SLE) and anti-phospholipid syndrome (APS). The aim of the present study was to investigate the association of PON1 Q192R and L55M polymorphisms with risk of SLE and associated APS among Egyptian sample. The study included 120 SLE patients (45 without APS and 75 with APS) and 120 healthy subjects. PON1 Q192R and L55M polymorphisms were genotyped by real-time PCR. No significant differences in Q192R genotypes or allele frequencies were found between patients and controls (p = 0...
February 9, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28179889/predictors-of-stress-in-college-students
#8
REVIEW
Dalia Saleh, Nathalie Camart, Lucia Romo
University students often face different stressful situations and preoccupations: the first contact with the university, the freedom of schedule organization, the selection of their master's degree, very selective fields, etc. The purpose of this study is to evaluate a model of vulnerability to stress in French college students. Stress factors were evaluated by a battery of six scales that was accessible online during 3 months. A total of 483 students, aged between 18 and 24 years (Mean = 20.23, standard deviation = 1...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28178239/eprs-is-a-critical-mtorc1-s6k1-effector-that-influences-adiposity-in-mice
#9
Abul Arif, Fulvia Terenzi, Alka A Potdar, Jie Jia, Jessica Sacks, Arnab China, Dalia Halawani, Kommireddy Vasu, Xiaoxia Li, J Mark Brown, Jie Chen, Sara C Kozma, George Thomas, Paul L Fox
Metabolic pathways that contribute to adiposity and ageing are activated by the mammalian target of rapamycin complex 1 (mTORC1) and p70 ribosomal protein S6 kinase 1 (S6K1) axis. However, known mTORC1-S6K1 targets do not account for observed loss-of-function phenotypes, suggesting that there are additional downstream effectors of this pathway. Here we identify glutamyl-prolyl-tRNA synthetase (EPRS) as an mTORC1-S6K1 target that contributes to adiposity and ageing. Phosphorylation of EPRS at Ser999 by mTORC1-S6K1 induces its release from the aminoacyl tRNA multisynthetase complex, which is required for execution of noncanonical functions of EPRS beyond protein synthesis...
February 16, 2017: Nature
https://www.readbyqxmd.com/read/28177750/modulatory-role-of-co-enzyme-q10-on-methionine-and-choline-deficient-diet-induced-non-alcoholic-steatohepatitis-nash-in-albino-rats
#10
Dalia O Saleh, Rania F Ahmed, Mohamed M Amin
The present study aimed to evaluate the hepato-protective and neuro-protective activity of Co-enzyme Q10 (CoQ10) on non-alcoholic steatohepatitis (NASH) in albino rats induced by methionine and choline-deficient (MCD) diet. Rats were fed an MCD diet for 8 weeks to induce non-alcoholic steatohepatitis. CoQ10 (10 mg/(kg·day)(-1)) was orally administered for 2 consecutive weeks. Twenty-four hours after the last dose of the drug, the behavioral test, namely the activity cage test, was performed and the activity counts were recorded...
November 9, 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28177745/pre-and-within-meal-effects-of-fluid-dairy-products-on-appetite-food-intake-glycemia-and-regulatory-hormones-in-children
#11
Shirley Vien, Bohdan L Luhovyy, Barkha P Patel, Shirin Panahi, Dalia El Khoury, Rebecca C Mollard, Jill K Hamilton, G Harvey Anderson
The effect of beverages commonly consumed by children in-between or with meals on short-term food intake (FI) and glycemic control has received little attention. Therefore, two experiments were conducted in 9-14 y old children following a randomized repeated measures design. Experiment 1 (<i>n</i>=32) compared the effects of water (control) and isocaloric (130 kcal) amounts of 2% milk, chocolate milk, yogurt drink and fruit punch on subjective appetite and FI. Experiment 2 (<i>n</i>=20) compared the effects of isocaloric (130 kcal) amounts of 2% milk and fruit punch on subjective appetite, FI, glycemic and appetite hormone responses...
November 21, 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28174439/the-effect-of-kinesio-taping-with-exercise-compared-with-exercise-alone-on-pain-range-of-motion-and-disability-of-the-shoulder-in-postmastectomy-females-a-randomized-control-trial
#12
Sayed A Tantawy, Dalia M Kamel
[Purpose] The aim of the study was to investigate the effect of kinesio tape on pain, range of motion, and disability of the shoulder. [Subjects and Methods] Seventy-four female patients who underwent modified radical mastectomy participated in this study. They were randomly divided into two groups, an experimental group that received kinesio tape for the shoulder joint in addition to a conventional physiotherapy program and a control group that received the physiotherapy program only. Outcome measures included the Visual Analogue Scale, shoulder range of motion, and Shoulder Pain and Disability Index...
December 2016: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28164635/microrna-122-as-a-novel-non-invasive-marker-of-liver-fibrosis-in-hepatitis-c-virus-patients
#13
Ahmed A S Omran, Khalid S Osman, Hanan M Kamel, Emad A Abdel-Naem, Dalia E El-Deen Hasan
BACKGROUND: Noninvasive evaluation of hepatic fibrosis is a growing scientific effort in medicine and is of particular interest as early diagnosis is important for the timely prevention and treatment of liver fibrosis and cirrhosis. Non-invasive markers of liver fibrosis have recently been developed as an alternative to liver biopsy. Aim of the work: The aim of this study was to assess the diagnostic value of serum microRNA 122 in Egyptian chronic hepatitis C virus infected patients. This may be a useful tool as a non invasive diagnostic test to detect early stages of fibrosis in comparison to 4 non invasive indexes (APRI, Forns, FIB-4, and FI) vs...
July 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28160335/targeting-human-set1-mll-family-of-proteins
#14
REVIEW
Masoud Vedadi, Levi Blazer, Mohammad S Eram, Dalia Barsyte-Lovejoy, Cheryl H Arrowsmith, Taraneh Hajian
The SET1 family of proteins, and in particular MLL1, are essential regulators of transcription and key mediators of normal development and disease. Here, we summarize the detailed characterization of the methyltransferase activity of SET1 complexes and the role of the key subunits, WDR5, RbBP5, ASH2L and DPY30. We present new data on full kinetic characterization of human MLL1, MLL3, SET1A and SET1B trimeric, tetrameric and pentameric complexes to elaborate on substrate specificities and compare our findings with what has been reported before...
February 4, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28157763/effect-of-procedural-related-variables-on-melanocyte-keratinocyte-suspension-transplantation-in-nonsegmental-stable-vitiligo-a-clinical-and-immunocytochemical-study
#15
Bakr Mohamed El-Zawahry, Samia Esmat, Dalia Bassiouny, Naglaa Sameh Zaki, Rehab Sobhi, Marwah A Saleh, Dalia Abdel-Halim, Rehab Hegazy, Heba Gawdat, Nesrin Samir, Marwa El-Hawary, Zeinab El Maadawi, Heba Gouda, Mervat Khorshied
BACKGROUND: Melanocyte-keratinocyte suspension (M-K susp) is gaining popularity for vitiligo treatment. Few studies have addressed procedure-related variables. OBJECTIVE: To assess the effect of different M-K susp procedure-related variables on the clinical outcome in stable vitiligo. METHODS: This prospective multicenter comparative study included 40 cases with nonsegmental stable vitiligo. Donor site was either a skin graft in noncultured epidermal cell suspension (NCECS) or hair follicle units in outer root sheath hair follicle suspension (ORSHFS)...
February 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28157387/trigonelline-attenuates-hepatic-complications-and-molecular-alterations-in-high-fat-high-fructose-diet-induced-insulin-resistance-in-rats
#16
Nehal A Afifi, Amer Ramadan, Emad Y Erian, Dalia O Saleh, Ahmed A Sedik, Manal Badawi, Walid El Hotaby
The present study aimed to evaluate the effect of trigonelline (TRG) on the hepatic complications associated with high-fat high-fructose (HFHF) diet-induced insulin resistance (IR) in rats. IR was induced by giving a saturated fat diet and 10% fructose in drinking water to rats for 8 weeks. Insulin-resistant rats were orally treated with TRG (50 and 100 mg/kg), sitagliptin (SIT; 5 mg/kg), or a combination of TRG (50 mg/kg) and SIT (5 mg/kg) for 14 days. Liver homogenates were used for assessment of hepatic lipids, oxidative stress biomarkers, and inflammatory cytokines...
October 12, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28154192/streptococcus-pyogenes-endopeptidase-o-contributes-to-evasion-from-complement-mediated-bacteriolysis-via-binding-to-human-complement-factor-c1q
#17
Mariko Honda-Ogawa, Tomoko Sumitomo, Yasushi Mori, Dalia Talat Hamd, Taiji Ogawa, Masaya Yamaguchi, Masanobu Nakata, Shigetada Kawabata
Streptococcus pyogenes secrets various virulence factors for evasion from complement-mediated bacteriolysis. However, full understanding of the molecules possessed by this organism that interact with complement C1q, an initiator of the classical complement pathway, remains elusive. In this study, we identified an endopeptidase of S. pyogenes, PepO, as an interacting molecule, and investigated its effects on complement immunity and pathogenesis. Enzyme-linked immunosorbent assay and surface plasmon resonance analysis findings revealed that S...
January 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28149821/usefulness-of-full-outline-of-unresponsiveness-score-to-predict-extubation-failure-in-intubated-critically-ill-patients-a-pilot-study
#18
Tarek Said, Anis Chaari, Karim Abdel Hakim, Dalia Hamama, William Francis Casey
OBJECTIVE: To assess the usefulness of the full outline of unresponsiveness (FOUR) score in predicting extubation failure in critically ill intubated patients admitted with disturbed level of conscious in comparison with the Glasgow coma scale (GCS). PATIENTS AND METHODS: All intubated critically ill patients with a disturbed level of consciousness were assessed using both the FOUR score and the GCS. The FOUR score and the GCS were compared regarding their predictive value for successful extubation at 14 days after intubation as a primary outcome measure...
October 2016: International Journal of Critical Illness and Injury Science
https://www.readbyqxmd.com/read/28149778/epidemiological-clinical-and-laboratory-findings-of-infectious-keratitis-at-mansoura-ophthalmic-center-egypt
#19
Amani E Badawi, Dalia Moemen, Nora L El-Tantawy
AIM: To analyze the epidemiological, clinical and laboratory findings of infectious keratitis. METHODS: A retrospective study on cases of infective keratitis, attended our institution from Mar. 2013 to Feb. 2015, was done at Mansoura Ophthalmic Center, Egypt. Corneal scrapings were performed and processed for direct microscopy and culture in appropriate media using standard laboratory protocols. RESULTS: Out of 245 patients enrolled for study, 247 corneal scrapings were obtained...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28144903/meeting-the-aichi-targets-pushing-for-zero-extinction-conservation
#20
REVIEW
Stephan M Funk, Dalia Conde, John Lamoreux, John E Fa
Effective protection of the ~19 000 IUCN-listed threatened species has never been more pressing. Ensuring the survival of the most vulnerable and irreplaceable taxa and places, such as those identified by the Alliance for Zero Extinction (AZE) species and their associated sites (AZEs&s), is an excellent opportunity to achieve the Aichi 2020 Targets T11 (protected areas) and T12 (preventing species extinctions). AZE taxa have small, single-site populations that are especially vulnerable to human-induced extinctions, particularly for the many amphibians...
January 31, 2017: Ambio
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