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https://www.readbyqxmd.com/read/27931515/autologous-adipose-derived-mesenchymal-stromal-cells-for-the-treatment-of-psoriasis-vulgaris-and-psoriatic-arthritis-a-case-report
#1
Miguel M De Jesus, Jayson S Santiago, Camille V Trinidad, Melvin E See, Kimberly R Semon, Manuel O Fernandez, Francisco S Chung
Psoriasis is a dermatologic disease of immune origins with no definitive cure. We report the Makati Medical Center experience of utilizing autologous mesenchymal stromal cells (MSCs) for one patient with psoriasis vulgaris (PV) and another with psoriatic arthritis (PA). Patients were educated and gave informed consent, according to the principles of the Declaration of Helsinki. The protocol was approved by the Cellular Transplantation Ethics Committee of the Makati Medical Center. Autologous MSCs were cultured from lipoaspirate and expanded in a clean room class 100 facility (Cellular Therapeutics Center, Makati Medical Center)...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27931179/structural-and-functional-integrity-of-the-intraparietal-sulcus-in-moderate-and-severe-traumatic-brain-injury
#2
Chandler Sours, Prashant Raghavan, Alexandre Medina de Jesus, Steven R Roys, Li Jiang, Jiachen Zhuo, Rao P Gullapalli
Severe and Moderate TBI (sTBI) often results in long-term cognitive deficits such as reduced processing speed and attention. The intraparietal sulcus (IPS) is a neocortical structure that plays a crucial role in the deeply interrelated processes of multisensory processing and top down attention. Therefore, we hypothesized that disruptions in the functional and structural connections of the IPS may play a role in the development of such deficits. To examine these connections we used resting state fMRI (rsfMRI) and diffusion kurtosis imaging (DKI) in a cohort of 27 sTBI patients (29...
December 8, 2016: Journal of Neurotrauma
https://www.readbyqxmd.com/read/27928864/vaginal-misoprostol-and-cervical-ripening-balloon-for-induction-of-labor-in-late-term-pregnancies
#3
Jorge Duro Gómez, María Fernanda Garrido Oyarzún, Ana Belén Rodríguez Marín, Antonio Jesús de la Torre González, José Eduardo Arjona Berral, Camil Castelo-Branco
AIM: The aim of this study was to compare vaginal misoprostol with the Cook cervical ripening balloon (CCRB) for induction of labor in late-term nulliparous women. METHODS: This open, quasi-experimental, prospective study included 109 nulliparous women with late-term pregnancies and Bishop scores < 7. Fifty-five women were allocated to receive vaginal misoprostol 25 mcg and 54 received the CCRB to induce labor. The primary outcome was the time until delivery...
December 8, 2016: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/27927163/phenotypic-and-molecular-detection-of-the-bla-kpc-gene-in-clinical-isolates-from-inpatients-at-hospitals-in-s%C3%A3-o-luis-ma-brazil
#4
Patricia Cristina Saldanha Ribeiro, Andrea Souza Monteiro, Sirlei Garcia Marques, Sílvio Gomes Monteiro, Valério Monteiro-Neto, Martina Márcia Melo Coqueiro, Ana Cláudia Garcia Marques, Rosimary de Jesus Gomes Turri, Simone Gonçalves Santos, Maria Rosa Quaresma Bomfim
BACKGROUND: Bacteria that produce Klebsiella pneumoniae carbapenemases (KPCs) are resistant to broad-spectrum β-lactam antibiotics. The objective of this study was to phenotypically and genotypically characterize the antibiotic susceptibility to carbapenems of 297 isolates recovered from clinical samples obtained from inpatients at 16 hospitals in São Luis (Maranhão, Brazil). METHODS: The study was conducted using phenotypic tests and molecular methods, including polymerase chain reaction (PCR), sequencing and enterobacterial repetitive intergenic consensus (ERIC)-PCR...
December 7, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27926846/alamethicin-supramolecular-organization-in-lipid-membranes-from-19-f-solid-state-nmr
#5
Evgeniy S Salnikov, Jesus Raya, Marta De Zotti, Ekaterina Zaitseva, Cristina Peggion, Gema Ballano, Claudio Toniolo, Jan Raap, Burkhard Bechinger
Alamethicins (ALMs) are antimicrobial peptides of fungal origin. Their sequences are rich in hydrophobic amino acids and strongly interact with lipid membranes, where they cause a well-defined increase in conductivity. Therefore, the peptides are thought to form transmembrane helical bundles in which the more hydrophilic residues line a water-filled pore. Whereas the peptide has been well characterized in terms of secondary structure, membrane topology, and interactions, much fewer data are available regarding the quaternary arrangement of the helices within lipid bilayers...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27925073/frequency-of-gastrointestinal-parasites-in-cats-seen-at-the-university-of-s%C3%A3-o-paulo-veterinary-hospital-brazil
#6
Solange Maria Gennari, Juliana Isabel Giuli da Silva Ferreira, Hilda Fátima de Jesus Pena, Marcelo Bahia Labruna, Sérgio Dos Santos Azevedo
The frequency of gastrointestinal infections in 502 cats seen at the Veterinary Hospital of the University of São Paulo, SP, Brazil, between 2005 and 2014, was measured. The samples were analyzed using methods of flotation and sedimentation. The results were compared with those from studies published previously using fecal samples from the same hospital at different times. Associations between the frequency of positivity for each parasite and age, breed, sex, diarrhea and use of anthelmintic were investigated (chi-square or Fisher's exact tests)...
December 1, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27923147/validation-of-the-portuguese-version-of-the-pittsburgh-sleep-quality-index-psqi-pt
#7
Karine Alexandra Del Rio João, Nathália Brandolim Becker, Saul de Neves Jesus, Rute Isabel Santos Martins
The present study was realised to validate the Portuguese version of the PSQI. The instrument PSQI-PT was applied to 347 Portuguese community-dwelling adults aged 18-69 years old. The resulting data was used to perform the psychometric analysis to validate the instrument. No structural modifications to the questionnaire were necessary during the adaptation process. The scores for the PSQI-PT showed an adequate internal consistency. The principal component analysis (PCA) produced good factor loading for all items...
November 28, 2016: Psychiatry Research
https://www.readbyqxmd.com/read/27922332/effect-of-moderate-and-high-intensity-chronic-exercise-on-the-pancreatic-islet-morphometry-in-healthy-rats-bdnf-receptor-participation
#8
Alberto Jiménez-Maldonado, Adolfo Virgen-Ortiz, Valery Melnikov, Alejandrina Rodríguez-Hernández, Armando Gamboa-Domínguez, Sergio Montero, Jesús Muñiz-Murguía, Mónica Lemus, Elena Roces de Álvarez-Buylla
A B S T R A C T The function and morphology of β-cells is largely dependent on insulin demand. As β-cells cover a bigger cell proportion in pancreas islets, changes of insulin producer cells affect the whole pancreatic islet morphology. Growth factors as the neurotrophins regulate the pancreas physiology, besides; physical exercise increases insulin sensitivity, and further modifies brain derived neurotrophic factor (BDNF) concentration in plasma. The aim of this study was to investigate the effects of chronic exercise (running in a treadmill for eight weeks) intensity on pancreatic islet morphometry in healthy state...
December 6, 2016: Islets
https://www.readbyqxmd.com/read/27922211/transrectal-ultrasonography-of-equine-lumbosacral-nerves-pilot-study-in-28-healthy-warmblood-horses
#9
Pablo Espinosa, Philippe Benoit, Isabel Salazar, Jesús de la Fuente, Philippe Heiles
A noninvasive method for visualizing lumbosacral nerves would be helpful for horses with suspected lumbosacral plexopathy or injury. The aim of this prospective descriptive pilot study was to characterize the ultrasonographic appearance of the lumbosacral nerves in a sample of healthy horses, and expand on the technique for image acquisition. Horses were recruited for inclusion if they were determined to be healthy and sound based on clinical and lameness evaluation. Transrectal ultrasound images of the lumbosacral nerves (L6, S1, and S2) were obtained for both sides...
December 6, 2016: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/27920729/transcriptional-network-architecture-of-breast-cancer-molecular-subtypes
#10
Guillermo de Anda-Jáuregui, Tadeo E Velázquez-Caldelas, Jesús Espinal-Enríquez, Enrique Hernández-Lemus
Breast cancer heterogeneity is evident at the clinical, histological and molecular level. High throughput technologies allowed the identification of intrinsic subtypes that capture transcriptional differences among tumors. A remaining question is whether said differences are associated to a particular transcriptional program which involves different connections between the same molecules. In other words, whether particular transcriptional network architectures can be linked to specific phenotypes. In this work we infer, construct and analyze transcriptional networks from whole-genome gene expression microarrays, by using an information theory approach...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27919214/hydrogel-based-drug-delivery-systems-a-review-with-special-emphasis-on-challenges-associated-with-decontamination-of-hydrogels-and-biomaterials
#11
Irene Satiko Kikuchi, Raquel Galante, Kamal Dua, Daniela Dal Molim Ghisleni, Terezinha de Jesus Andreoli Pinto, Venkata Ramana Malipeddi, Rajendra Awasthi
Many researches involving the development of new techniques and biomaterials to formulate a suitable drug delivery systems and tissue engineering have been conducted. The majority of published literature from these researches emphasizes the production and materials characterization. However there is very limited information regarding their sterilization methods. The present review article has summarized various different literatures that encompasses the difficulties encountered with sterilization methods in the preparations of hydrogels for biological applications...
December 5, 2016: Current Drug Delivery
https://www.readbyqxmd.com/read/27916844/effect-of-thermoultrasound-on-the-antioxidant-compounds-and-fatty-acid-profile-of-blackberry-rubus-fruticosus-spp-juice
#12
José de Jesús Manríquez-Torres, José Antonio Sánchez-Franco, Esther Ramírez-Moreno, Nelly Del Socorro Cruz-Cansino, José Alberto Ariza-Ortega, Jesús Martín Torres-Valencia
Blackberry (Rubus fruticosus spp.) fruit has high antioxidant activity due to its significant content of anthocyanins and antioxidant compounds. Among emerging technologies for food preservation, thermoultrasound is a technique that reduces microbial loads and releases compounds with antioxidant properties. The objective of this study was to determine the antioxidant content and fatty acid profile of blackberry juice subjected to thermoultrasound treatment in comparison to pasteurized juice. Blackberry juice and n-hexane extracts from a control (untreated juice), pasteurized, and thermoultrasonicated samples were evaluated for antioxidant activity, fatty acid profile, and antioxidant content...
November 29, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27916690/acyl-homoserine-lactone-changes-the-abundance-of-proteins-and-the-levels-of-organic-acids-associated-with-stationary-phase-in-salmonella-enteritidis
#13
Felipe Alves de Almeida, Natan de Jesus Pimentel Filho, Lanna Clícia Carrijo, Cláudia Braga Pereira Bento, Maria Cristina Baracat-Pereira, Uelinton Manoel Pinto, Leandro Licursi de Oliveira, Maria Cristina Dantas Vanetti
Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12-HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella...
December 1, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27914990/presence-of-melanopsin-in-human-crystalline-lens-epithelial-cells-and-its-role-in-melatonin-synthesis
#14
Hanan Awad Alkozi, Xiaoyu Wang, Maria Jesus Perez de Lara, Jesus Pintor
Melanopsin is a non-image forming photoreceptor known to be present in the retina and it is considered to have light regulated tasks among other functions. In the present work, melanopsin presence in human lens epithelial cells as well as in human lens tissue is described for the first time. Moreover, studying the concentration of melatonin and its synthesising enzyme AANAT proved a clear link between melanopsin activation and the suppression of melatonin synthesis. Melanopsin sensitivity to specific wavelength (465-480 nm, blue) was confirmed after making temporal studies incubating lens epithelial cells under light, red, green, blue and total darkness for 2, 4, 8, 12 h and analysing the concentration of both melatonin and its synthesising enzyme AANAT, discovering that melatonin levels after submitting cells to total darkness are significantly higher to ones submitted to white or specifically blue light (***p < 0...
November 30, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27914752/utility-of-bronchoalveolar-lavage-for-the-diagnosis-of-asbestos-related-diseases
#15
María Jesús Cruz, Victor Curull, Lara Pijuan, Daniel Álvarez-Simón, Albert Sánchez-Font, Javier de Gracia, Mario Culebras, Jaume Ferrer
INTRODUCTION: Bronchoalveolar lavage (BAL) analysis has been proposed as an objective technique for confirming asbestos exposure. However, the reliability and diagnostic yield of this procedure has not been studied in Spain. The aim of this study was to assess the usefulness of the analysis of asbestos bodies (AB) in bronchoalveolar lavage (BAL) for the diagnosis of asbestos-related diseases (ARD). METHODS: BAL samples from 72 patients (66 male, mean age 66 years) undergoing bronchoscopy were analyzed...
November 30, 2016: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/27914749/-development-and-pilot-study-of-a-questionnaire-to-assess-child-and-teenager-satisfaction-with-their-stay-in-hospital
#16
Montserrat Gómez de Terreros Guardiola, José Francisco Lozano Oyola, Isabel Avilés Carvajal, Rafael Jesús Martínez Cervantes
OBJECTIVE: To develop an instrument to assess the satisfaction of children and teenagers with their stay in hospital. METHODS: A qualitative analysis of hospitalisation satisfaction dimensions based on the feedback of hospitalised children and teenagers; a content validation study by a group of experts of the items generated for the different satisfaction dimensions; and a pilot study to assess the usefulness of the questionnaire with a sample of 84 children and teenagers hospitalised in Andalusia...
November 30, 2016: Gaceta Sanitaria
https://www.readbyqxmd.com/read/27914065/computational-design-of-membrane-curvature-sensing-peptides
#17
Armando Jerome de Jesus, Hang Yin
Computer simulations have become an indispensable tool in studying molecular biological systems. The unmatched spatial and temporal resolution that it offers enables for microscopic-level views into the dynamics and mechanics of biological systems. Recent advances in hardware resources have also opened up to computer simulations the investigation of longer timescale biological processes and larger systems. The study of membrane proteins or peptides especially benefits from simulations due to difficulties related to crystallization of such proteins in a membrane environment...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27912150/calea-uniflora-less-attenuates-the-inflammatory-response-to-carrageenan-induced-pleurisy-in-mice
#18
Julia Salvan da Rosa, Silvana Virginia Gagliotti Vigil de Mello, Geison Vicente, Yeo Jim K Moon, Felipe Perozzo Daltoé, Tamires Cardoso Lima, Rafaela de Jesus Souza, Maique Weber Biavatti, Tânia Silvia Fröde
Calea uniflora Less. (family Asteraceae), also named "arnica" and "erva-de-lagarto", is a native plant to the South and Southeast of Brazil. This species was used to treat rheumatism, respiratory diseases, and digestive problems in Brazilian folk medicine. In vitro studies have shown the important biological effects of C. uniflora. However no studies have focused on the mechanism of action of anti-inflammatory activity of C. uniflora. The aim of this study was to evaluate the anti-inflammatory effects of the crude extract, its fractions, and isolated compounds obtained from of C...
November 29, 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27912077/nlrp3-activation-induces-il-18-synthesis-and-affects-the-epithelial-barrier-function-in-reactive-cholangiocytes
#19
Luca Maroni, Laura Agostinelli, Stefania Saccomanno, Claudio Pinto, Debora Giordano, Chiara Rychlicki, Samuele De Minicis, Luciano Trozzi, Jesus M Banales, Espen Melum, Tom H Karlsen, Antonio Benedetti, Gianluca Svegliati Baroni, Marco Marzioni
Microbial products are thought to influence the progression of cholangiopathies, in particular primary sclerosing cholangitis (PSC). Inflammasomes are molecular platforms that respond to microbial products through the synthesis of proinflammatory cytokines. We investigated the role of inflammasome activation in cholangiocyte response to injury. Nucleotide-binding oligomerization domain (NOD)-like receptor family, pyrin domain-containing protein 3 (Nlrp3) expression was tested in cholangiocytes of normal and cholestatic livers...
November 29, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27903619/interleukin-10-mediated-regenerative-postnatal-tissue-repair-is-dependent-on-regulation-of-hyaluronan-metabolism-via-fibroblast-specific-stat3-signaling
#20
Swathi Balaji, Xinyi Wang, Alice King, Louis D Le, Sukanta S Bhattacharya, Chad M Moles, Manish J Butte, Vinicio A de Jesus Perez, Kenneth W Liechty, Thomas N Wight, Timothy M Crombleholme, Paul L Bollyky, Sundeep G Keswani
The cytokine IL-10 has potent antifibrotic effects in models of adult fibrosis, but the mechanisms of action are unclear. Here, we report a novel finding that IL-10 triggers a signal transducer and activator of transcription 3 (STAT3)-dependent signaling pathway that regulates hyaluronan (HA) metabolism and drives adult fibroblasts to synthesize an HA-rich pericellular matrix, which mimics the fetal regenerative wound healing phenotype with reduced fibrosis. By using cre-lox-mediated novel, inducible, fibroblast-, keratinocyte-, and wound-specific STAT3 knockdown postnatal mice-plus syngeneic fibroblast cell-transplant models-we demonstrate that the regenerative effects of IL-10 in postnatal wounds are dependent on HA synthesis and fibroblast-specific STAT3-dependent signaling...
November 30, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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