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miRNA Bladder

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https://www.readbyqxmd.com/read/28109084/mir-5195-3p-inhibits-proliferation-and-invasion-of-human-bladder-cancer-cells-by-directly-targeting-oncogene-klf5
#1
Zhangjie Jiang, Yida Zhang, Runfu Cao, Li Li, Kezhao Zhong, Qingsheng Chen, Jianjun Xiao
MiRNAs played key roles in carcinogenesis of many cancers including bladder cancer. In current study, the role of miR-5195-3p, a quite recently discovered and poorly studied miRNA, in the proliferation and invasion of human bladder cancer cells was investigated. Our data displayed that, compared with the healthy volunteers (control) and SU-HUC-1 human normal bladder epithelial cells, miR-5195-3p was sharply down-regulated in the bladder cancer patients and 5 human bladder cancer cell lines. Then, the oligo miR-5195-3p mimic or miR-5195-3p antagomir was respectively transfected into both T24 and BIU-87 bladder cancer cell lines...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28060766/opposing-roles-of-pik3ca-gene-alterations-to-ezh2-signaling-in-non-muscle-invasive-bladder-cancer
#2
Cristina Segovia, Mónica Martínez-Fernández, Marta Dueñas, Carolina Rubio, Fernando F López-Calderón, Clotilde Costa, Cristina Saiz-Ladera, María Fernández-Grajera, José Duarte, Huberto García Muñoz, Federico de la Rosa, Felipe Villacampa, Daniel Castellano, Jesús M Paramio
The high rates of tumor recurrence and progression represent a major clinical problem in non-muscle invasive bladder cancer. Previous data showed that EZH2-dependent signaling mediates these processes, whereas the frequent alterations of PIK3CA gene (copy gains and mutations) are predictive of reduced recurrence. Here we show, using clinical samples and bladder cancer cell lines, a functional interaction between EZH2- and PIK3CA-dependent signaling pathways. PIK3CA alterations mediated, on the one hand, the increased expression of two miRNAs, miR-101 and miR-138, which posttranscriptionally downregulate EZH2 expression...
January 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28042869/genome-wide-screen-of-mirnas-and-targeting-mrnas-reveals-the-negatively-regulatory-effect-of-mir-130b-3p-on-pten-by-pi3k-and-integrin-%C3%AE-1-signaling-pathways-in-bladder-carcinoma
#3
Mengxin Lv, Zhenyu Zhong, Hong Chi, Mengge Huang, Rong Jiang, Junxia Chen
miRNAs have emerged as promising markers for tumors. However, the underlying mechanism of specific miRNAs in bladder cancer (BC) remains largely unknown. Here, a comprehensive miRNA/mRNA expression profile was executed by microarray assay for four pairs of bladder carcinoma and para-carcinoma tissues from patients with grade 2 (G2) T2. A total of 99 miRNAs and 4416 mRNAs were discovered to be significantly differentially expressed in BC tissues compared with controls. Five microRNAs and two mRNAs were validated by qRT-PCR in 30 pairs of samples, including G1-G3/T1-T4...
December 31, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28000900/mir-24-3p-regulates-bladder-cancer-cell-proliferation-migration-invasion-and-autophagy-by-targeting-dedd
#4
Guoqiang Yu, Zhaohui Jia, Zhongling Dou
microRNAs (miRNAs), a class of small non-coding RNA molecules, can regulate gene expression by interacting with the 3'-untranslated regions (3'UTR) of target genes and influence various biological processes. We investigated the potential role of miR-24-3p in the development of bladder cancer by regulating DEDD, a member of the death effector domain-containing protein family. First, we found that miR-24-3p was highly expressed and that DEDD was expressed at a low level in bladder cancer tissues compared with that in adjacent bladder tissues by qRT-PCR (P<0...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/27998761/lncrna-spry4-it1-sponges-mir-101-3p-to-promote-proliferation-and-metastasis-of-bladder-cancer-cells-through-up-regulating-ezh2
#5
Dong Liu, Yawei Li, Gang Luo, Xingyuan Xiao, Dan Tao, Xinchao Wu, Miao Wang, Chao Huang, Liang Wang, Fuqing Zeng, Guosong Jiang
Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4-IT1 predicates poor prognosis and promotes tumor progress in several cancers. However, the underlying mechanism of SPRY4-IT1 in bladder cancer remains unknown. In this study, we found that SPRY4-IT1 knockdown induced inhibition of cell proliferation, cell migration and invasion ability, and caused promotion of apoptosis in bladder cancer both in vitro and in vivo...
December 18, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27983635/new-cross-talk-layer-between-ultraconserved-non-coding-rnas-micrornas-and-polycomb-protein-yy1-in-bladder-cancer
#6
Sara Terreri, Montano Durso, Vincenza Colonna, Alessandra Romanelli, Daniela Terracciano, Matteo Ferro, Sisto Perdonà, Luigi Castaldo, Ferdinando Febbraio, Filomena de Nigris, Amelia Cimmino
MicroRNAs (miRNAs) are highly conserved elements in mammals, and exert key regulatory functions. Growing evidence shows that miRNAs can interact with another class of non-coding RNAs, so-called transcribed ultraconserved regions (T-UCRs), which take part in transcriptional, post-transcriptional and epigenetic regulation processes. We report here the interaction of miRNAs and T-UCRs as a network modulating the availability of these non-coding RNAs in bladder cancer cells. In our cell system, antagomiR-596 increased the expression of T-UCR 201+...
December 14, 2016: Genes
https://www.readbyqxmd.com/read/27978829/mir-138-5p-contributes-to-cell-proliferation-and-invasion-by-targeting-survivin-in-bladder-cancer-cells
#7
Rong Yang, Minghui Liu, Hongwei Liang, Suhan Guo, Xu Guo, Min Yuan, Huibo Lian, Xiang Yan, Shiwei Zhang, Xi Chen, Feng Fang, Hongqian Guo, Chenyu Zhang
BACKGROUND: Survivin (encoded by the gene BIRC5) plays an important role in the carcinogenesis of bladder cancer. Identifying miRNAs that target Survivin in the setting of bladder cancer will help to develop Survivin-based therapies for bladder cancer. METHODS: The expression levels of miR-138-5p and Survivin protein were measured in 12 resected bladder cancer specimens. The correlation between miR-138-5p and Survivin was further examined by evaluating Survivin expression in human bladder cancer cell lines that either overexpressed or knocked down miR-138-5p...
December 15, 2016: Molecular Cancer
https://www.readbyqxmd.com/read/27973407/comparative-mirna-analysis-of-urine-extracellular-vesicles-isolated-through-five-different-methods
#8
Felix Royo, Izzuddin Diwan, Michael R Tackett, Patricia Zuñiga, Pilar Sanchez-Mosquera, Ana Loizaga-Iriarte, Aitziber Ugalde-Olano, Isabel Lacasa, Amparo Perez, Miguel Unda, Arkaitz Carracedo, Juan M Falcon-Perez
Urine extracellular vesicles are a valuable low-invasive source of information, especially for the cells of the genitourinary tract. In the search for biomarkers, different techniques have been developed to isolate and characterize the cargo of these vesicles. In the present work, we compare five of these different isolation methods (three commercial isolation kits, ultracentrifugation, and lectin-based purification) and perform miRNA profiling using a multiplex miRNA assay. The results showed high correlation through all isolation techniques, and 48 out of 68 miRNAs were detected above the detection limit at least 10 times...
December 10, 2016: Cancers
https://www.readbyqxmd.com/read/27922690/microrna-152-inhibits-tumor-cell-growth-by-directly-targeting-rtkn-in-hepatocellular-carcinoma
#9
Jiejing Zhou, Yanjun Zhang, Yuhong Qi, Dequan Yu, Qiuju Shao, Jun Liang
Hepatocellular carcinoma (HCC) is the most common form of adult liver cancer and accounts for approximately 90% of all cases of primary liver cancer annually. Rhotekin (RTKN), which functions as a cancer promoter, can be frequently detected in many human cancers, including gastric cancer, colorectal carcinoma and bladder carcinoma. The aim of this study was to investigate the role of RTKN in HCC. Using HCC cells and tissues from patients with liver cancer, we demonstrated that RTKN was significantly increased in HCC...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/27904689/mir-4295-promotes-cell-growth-in-bladder-cancer-by-targeting-btg1
#10
Yong-Hao Nan, Jun Wang, Yao Wang, Peng-Hao Sun, Yu-Ping Han, Li Fan, Kai-Chen Wang, Fu-Jun Shen, Wei-Hua Wang
microRNAs (miRNAs) have been demonstrated to contribute to tumor progression and metastasis, and have been proposed to be key regulators of diverse biological processes. In this study, we report that miR-4295 is deregulated in bladder cancer tissues and cell lines. To characterize the role of miR-4295 in bladder cancer cells, we performed functional assays. The overexpression of miR-4295 significantly promoted bladder cancer cell proliferation, colony formation, and migration. Moreover, its downregulation induced cell cycle arrest and apoptosis of bladder cancer cells...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904673/microrna-206-acts-as-a-tumor-suppressor-in-bladder-cancer-via-targeting-yrdc
#11
Bisheng Huang, Wei Zhai, Guanghui Hu, Chi Huang, Tiancheng Xie, Jingwei Zhang, Yunfei Xu
Accumulating evidence suggested that microRNA (miRNA) plays important regulatory roles in the initiation and development of various cancers. Previous study showed that microRNA-206 (miR-206) is dysregulated in human bladder cancer tissues, however, the biological function and underlying mechanisms of miR-206 in human bladder cancer remain unknown. In the present study, we aimed to investigate the clinical significance of miR-206 and its target gene YRDC in human bladder cancer, and to determine its effects on oncogenic phenotypes of this disease...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27863388/comprehensive-analysis-of-aberrantly-expressed-profiles-of-lncrnas-and-mirnas-with-associated-cerna-network-in-muscle-invasive-bladder-cancer
#12
Hanbo Wang, Leilei Niu, Shaobo Jiang, Jing Zhai, Ping Wang, Feng Kong, Xunbo Jin
Although initially thought to be transcriptional noise, long noncoding RNAs (lncRNAs) are gaining increased attention in human cancers as its diversity function. At present, lncRNAs are regarded as the main part of competing endogenous RNA (ceRNA) network due to its regulation on protein-coding gene expression by acting as miRNA sponges. However, functional roles of lncRNA-mediated ceRNAs in muscle-invasive bladder cancer remain unclear. To clarify relevant potential mechanisms, here we comprehensively compared the expression profiles of mRNAs, lncRNAs and miRNAs between 322 muscle-invasive bladder cancer tissues and 19 non-tumor bladder tissues, based on the Cancer Genome Atlas (TCGA)...
November 15, 2016: Oncotarget
https://www.readbyqxmd.com/read/27845906/specific-micro-rna-expression-patterns-distinguish-the-basal-and-luminal-subtypes-of-muscle-invasive-bladder-cancer
#13
Andrea E Ochoa, Woonyoung Choi, Xiaoping Su, Arlene Siefker-Radtke, Bogdan Czerniak, Colin Dinney, David J McConkey
The roles of non-coding RNAs in controlling clinical and biological heterogeneity in bladder cancer remain unclear. We used TCGA's published dataset (n = 405 tumors) as a discovery cohort and created a new validation cohort to define the miRNA expression patterns in the basal and luminal molecular subtypes of muscle-invasive bladder cancer (MIBC). We identified 63 miRNAs by PAM, which optimally identified basal and luminal tumors. The targets of the top luminal miRNAs were activators of EMT (ZEB1, ZEB2) and basal subtype transcription (IL-6, EGFR, STAT3), whereas the targets of the top basal miRNAs were involved in adipogenesis pathways and luminal breast cancer (ERBB2, ERBB3)...
November 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27820650/upregulation-of-mir-3658-in-bladder-cancer-and-tumor-progression
#14
Y J Chen, H F Wang, M Liang, R C Zou, Z R Tang, J S Wang
Despite increasing advances in surgical techniques and adjuvant chemotherapies, bladder cancer remains the ninth leading cause of male malignancy-associated deaths worldwide. Several microRNAs (miRNAs) have been identified to be closely associated with the progression and prognosis of, and response to treatments in various human cancers. However, few studies have investigated the role of miR-3658 in bladder cancer. In this study, we examined the expression of miR-3658 in 96 pairs of bladder cancer tissues and adjacent non-tumor tissues via quantitative reverse-transcription polymerase chain reaction...
November 3, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27814720/mir-199a-5p-suppresses-human-bladder-cancer-cell-metastasis-by-targeting-ccr7
#15
Mi Zhou, Shuai Wang, Linyi Hu, Feng Liu, Qi Zhang, Dahong Zhang
BACKGROUND: C-C chemokine receptor type 7 (CCR7) overexpression correlated with lymphatic metastasis and poor prognosis is a major obstacle to bladder cancer treatment. Recent studies have revealed that miR-199a-5p was significantly abnormal expressed in several solid tumors and functioned as oncogene or tumor suppressor. This study was aimed to further investigate the effects of miR-199a-5p on the cell metastasis mediated by CCR7 in bladder cancer. METHODS: Quantitative Real Time PCR (qRT-PCR) was firstly performed to identified the expression of miR-199a-5p and CCR7 in human bladder cancer samples and cell lines...
November 4, 2016: BMC Urology
https://www.readbyqxmd.com/read/27804986/the-translational-potential-of-micrornas-as-biofluid-markers-of-urological-tumours
#16
REVIEW
Annika Fendler, Carsten Stephan, George M Yousef, Glen Kristiansen, Klaus Jung
MicroRNAs (miRNAs) are secreted by cells in vesicles, bound in a ribonucleoprotein complex or as free molecules. These miRNA secretion pathways are dysregulated in cancer, making miRNAs attractive candidate molecules for liquid biopsies. A number of studies have investigated the regulation of miRNA secretion into blood and urine and suggested that miRNAs are noninvasive diagnostic, prognostic and surveillance markers in urological carcinomas, and research in this area has increased over the past 5 years. However, methodological and analytical pitfalls exist and require addressing to enable future translation of the laboratory findings regarding miRNAs as biomarkers into clinical practice in bladder cancer, kidney cancer, prostate cancer and testicular cancer...
December 2016: Nature Reviews. Urology
https://www.readbyqxmd.com/read/27789288/bladder-overactivity-involves-overexpression-of-microrna-132-and-nerve-growth-factor
#17
Mahendra Kashyap, Subrata Pore, Michael Chancellor, Naoki Yoshimura, Pradeep Tyagi
AIM: Here, we assessed the expression of non-protein coding microRNAs (miRs), nerve growth factor and inflammatory molecules in the rat model of acetic acid induced bladder overactivity. MAIN METHODS: Under isoflurane anesthesia, adult female Sprague-Dawley rats were instilled for 30min with either saline or NGF antisense oligonucleotide complexed with liposomes. 24h later, treated rats were exposed to either intravesical infusion of saline or saline containing 0...
December 15, 2016: Life Sciences
https://www.readbyqxmd.com/read/27753026/comparison-of-microrna-content-in-plasma-and-urine-indicates-the-existence-of-a-transrenal-passage-of-selected-micrornas
#18
Eva Pazourkova, Sarka Pospisilova, Iveta Svobodova, Ales Horinek, Antonin Brisuda, Viktor Soukup, Jan Hrbacek, Otakar Capoun, Jaroslav Mares, Tomas Hanus, Marek Babjuk, Marie Korabecna
MicroRNAs (miRNAs) in urine are examined as potential biomarkers. We examined the urine samples from 70 individuals (45 males, 25 females, mean age 65 years, range 20-84 years). Of the urine donors, 15 were healthy volunteers, 5 were patients with non-cancer diseases, 50 were patients with different stages of bladder cancer. To examine the spectrum of miRNAs in the cell-free fraction of urine, TaqMan Human miRNA Array Card A v.2.1 was used. A set of 30 miRNAs were found that are constantly present in urine supernatants independently of sex, age and health status of the subjects...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27752905/expression-of-mirna-630-in-bladder-urothelial-carcinoma-and-its-clinical-significance
#19
Zhi-Yu Wang, Wei Zhang, Jin-Jian Yang, Dong-Kui Song, Jin-Xing Wei
Many studies informed that microRNAs (miRNAs) could function as diagnostic and prognostic indicators in several cancers. The aims of this study were to explore the expression of miR-630 in bladder urothelial carcinoma and its clinical significance for the evaluation of cancer prognosis. A total of 116 patients with bladder urothelial carcinoma were obtained in this retrospective study between May, 2012 and Sep. 2015. Quantitative real-time PCR (qRT-PCR) was conducted to evaluate the expression level of miR-630...
October 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/27751843/extracellular-vesicles-as-a-source-for-non-invasive-biomarkers-in-bladder-cancer-progression
#20
Zoraida Andreu, Renan Otta Oshiro, Alberto Redruello, Soraya López-Martín, Cristina Gutiérrez-Vázquez, Esperanza Morato, Ana Isabel Marina, Carlos Olivier Gómez, María Yáñez-Mó
Bladder cancer is the second most frequent malignancy of the urinary tract after prostate cancer. Current diagnostic techniques, such as cystoscopy and biopsies are highly invasive and accompanied of undesirable side effects. Moreover, there are no suitable biomarkers for relapse or progression prognosis. We analysed whether the specific composition of microRNAs (miRNAs) and proteins of extracellular vesicles (EVs) that urothelial tumor cells of bladder mucosa release into the urine, could reflect their pathologic condition...
October 14, 2016: European Journal of Pharmaceutical Sciences
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