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https://www.readbyqxmd.com/read/27909649/in-silico-profiling-of-cell-growth-and-succinate-production-in-escherichia-coli-nzn111
#1
Xingxing Jian, Ningchuan Li, Cheng Zhang, Qiang Hua
BACKGROUND: Succinic acid is a valuable product due to its wide-ranging utilities. To improve succinate production and reduce by-products formation, Escherichia coli NZN111 was constructed by insertional inactivation of lactate dehydrogenase (LDH) and pyruvate formate lyase (PFL) encoded by the genes ldhA and pflB, respectively. However, this double-deletion mutant is incapable of anaerobically growing on glucose in rich or minimal medium even with acetate supplementation. A widespread hold view is that the inactivation of NADH-dependent LDH limits the regeneration of NAD(+) and consequently disables proper growth under anaerobic conditions...
2016: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/27847372/towards-a-standardized-method-for-broth-microdilution-susceptibility-testing-of-haemophilus-parasuis
#2
Sandra Prüller, Conny Turni, Patrick J Blackall, Martin Beyerbach, Günter Klein, Lothar Kreienbrock, Katrin Strutzberg-Minder, Heike Kaspar, Diana Meemken, Corinna Kehrenberg
Currently, there is no agreed method available for broth microdilution susceptibility testing of Haemophilus parasuis, one of the most important bacterial pathogens in pig production. Therefore, the aim of the study was to develop a method that could be easily performed by diagnostic laboratories and that appears suitable for a harmonized susceptibility testing.Growth determinations using one type strain and three field isolates revealed no visible growth of H. parasuis in media which have proven to be suitable for susceptibility testing of fastidious organisms...
November 9, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27826689/%C3%AE-hydroxybutyrate-in-the-brain-one-molecule-multiple-mechanisms
#3
Lavanya B Achanta, Caroline D Rae
β-Hydroxybutyrate (βOHB), a ketone body, is oxidised as a brain fuel. Although its contribution to energy metabolism in the healthy brain is minimal, it is an interesting metabolite which is not only oxidised but also has other direct and collateral effects which make it a molecule of interest for therapeutic purposes. In brain βOHB can be produced in astrocytes from oxidation of fatty acids or catabolism of amino acids and is metabolised in the mitochondria of all brain cell types although uptake across the blood brain barrier is a metabolic control point...
November 8, 2016: Neurochemical Research
https://www.readbyqxmd.com/read/27812097/investigation-of-factors-affecting-aerobic-and-respiratory-growth-in-the-oxygen-tolerant-strain-lactobacillus-casei-n87
#4
Rocco G Ianniello, Teresa Zotta, Attilio Matera, Francesco Genovese, Eugenio Parente, Annamaria Ricciardi
Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction...
2016: PloS One
https://www.readbyqxmd.com/read/27798266/focally-perfused-succinate-potentiates-brain-metabolism-in-head-injury-patients
#5
Ibrahim Jalloh, Adel Helmy, Duncan J Howe, Richard J Shannon, Peter Grice, Andrew Mason, Clare N Gallagher, Matthew G Stovell, Susan van der Heide, Michael P Murphy, John D Pickard, David K Menon, T Adrian Carpenter, Peter J Hutchinson, Keri Lh Carpenter
Following traumatic brain injury, complex cerebral energy perturbations occur. Correlating with unfavourable outcome, high brain extracellular lactate/pyruvate ratio suggests hypoxic metabolism and/or mitochondrial dysfunction. We investigated whether focal administration of succinate, a tricarboxylic acid cycle intermediate interacting directly with the mitochondrial electron transport chain, could improve cerebral metabolism. Microdialysis perfused disodium 2,3-(13)C2 succinate (12 mmol/L) for 24 h into nine sedated traumatic brain injury patients' brains, with simultaneous microdialysate collection for ISCUS analysis of energy metabolism biomarkers (nine patients) and nuclear magnetic resonance of (13)C-labelled metabolites (six patients)...
October 17, 2016: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/27757702/increasing-dissolved-oxygen-disrupts-iron-homeostasis-in-production-cultures-of-escherichia-coli
#6
Antonino Baez, Joseph Shiloach
The damaging effect of high oxygen concentration on growth of Escherichia coli is well established. Over-oxygenation increases the intracellular concentration of reactive oxygen species (ROS), causing the destruction of the [4Fe-4S] cluster of dehydratases and limiting the biosynthesis of both branched-chain amino acids and nicotinamide adenine dinucleotide. A key enzyme that reduces the damaging effect of superoxide is superoxide dismutase (SOD). Its transcriptional regulation is controlled by global transcription regulators that respond to changes in oxygen and iron concentrations and pH...
October 18, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27714559/caco3-supplementation-alleviates-the-inhibition-of-formic-acid-on-acetone-butanol-ethanol-fermentation-by-clostridium-acetobutylicum
#7
Gaoxiang Qi, Lian Xiong, Xiaoqing Lin, Chao Huang, Hailong Li, Xuefang Chen, Xinde Chen
OBJECTIVE: To investigate the inhibiting effect of formic acid on acetone/butanol/ethanol (ABE) fermentation and explain the mechanism of the alleviation in the inhibiting effect under CaCO3 supplementation condition. RESULTS: From the medium containing 50 g sugars l(-1) and 0.5 g formic acid l(-1), only 0.75 g ABE l(-1) was produced when pH was adjusted by KOH and fermentation ended prematurely before the transformation from acidogenesis to solventogenesis. In contrast, 11...
October 6, 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27637884/metabolism-of-fructophilic-lactic-acid-bacteria-isolated-from-apis-mellifera-l-bee-gut-a-focus-on-the-phenolic-acids-as-external-electron-acceptors
#8
Pasquale Filannino, Raffaella Di Cagno, Rocco Addante, Erica Pontonio, Marco Gobbetti
: Fructophilic lactic acid bacteria (FLAB) are strongly associated to the gastrointestinal tract (GIT) of Apis mellifera L. worker bees due to the consumption of fructose as a major carbohydrate. Seventy-seven presumptive lactic acid bacteria (LAB) were isolated from GIT of healthy A. mellifera L. adults, which were collected from 5 different geographical locations of Apulia region (Italy). Almost all the isolates showed fructophilic tendencies, which were identified as Lactobacillus kunkeei (69%) or Fructobacillus fructosus (31%)...
September 16, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27615794/arabidopsis-root-type-ferredoxin-nadp-h-oxidoreductase-2-is-involved-in-detoxification-of-nitrite-in-roots
#9
Takushi Hachiya, Nanae Ueda, Munenori Kitagawa, Guy Hanke, Akira Suzuki, Toshiharu Hase, Hitoshi Sakakibara
Ferredoxin : NADP(H) oxidoreductase (FNR) plays a key role in redox metabolism in plastids. Whereas leaf FNR (LFNR) is required for photosynthesis, root FNR (RFNR) is believed to provide electrons to ferredoxin (Fd)-dependent enzymes, including nitrite reductase (NiR) and Fd-glutamine-oxoglutarate aminotransferase (Fd-GOGAT) in non-photosynthetic conditions. In some herbal species, however, most nitrate reductase activity is located in photosynthetic organs, and ammonium in roots is assimilated mainly by Fd-independent NADH-GOGAT...
September 11, 2016: Plant & Cell Physiology
https://www.readbyqxmd.com/read/27594836/head-to-head-comparison-of-short-term-treatment-with-the-nad-precursor-nicotinamide-mononucleotide-nmn-and-6-weeks-of-exercise-in-obese-female-mice
#10
Golam M Uddin, Neil A Youngson, David A Sinclair, Margaret J Morris
Obesity is well known to be a major cause of several chronic metabolic diseases, which can be partially counteracted by exercise. This is due, in part, to an upregulation of mitochondrial activity through increased nicotinamide adenine dinucleotide (NAD(+)). Recent studies have shown that NAD(+) levels can be increased by using the NAD(+) precursor, nicotinamide mononucleotide (NMN) leading to the suggestion that NMN could be a useful intervention in diet related metabolic disorders. In this study we compared the metabolic, and especially mitochondrial-associated, effects of exercise and NMN in ameliorating the consequences of high-fat diet (HFD) induced obesity in mice...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27525823/role-of-mitochondrial-complex-i-and-protective-effect-of-coq10-supplementation-in-propofol-induced-cytotoxicity
#11
Christian Bergamini, Noah Moruzzi, Francesco Volta, Laura Faccioli, Jantje Gerdes, Maria Cristina Mondardini, Romana Fato
Propofol (2,6-diisopropylphenol) is an anaesthetic widely used for human sedation. Due to its intrinsic antioxidant properties, rapid induction of anaesthesia and fast recovery, it is employed in paediatric anaesthesia and in the intensive care of premature infants. Recent studies have pointed out that exposure to anaesthesia in the early stage of life might be responsible of long-lasting cognitive impairment. The apoptotic neurodegeneration induced by general anaesthetics (GA) involves mitochondrial impairment due to the inhibition of the OXPHOS machinery...
August 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27343380/-s-ys-51-a-novel-isoquinoline-alkaloid-attenuates-obesity-associated-non-alcoholic-fatty-liver-disease-in-mice-by-suppressing-lipogenesis-inflammation-and-coagulation
#12
Eun Jung Park, Young Min Kim, Hye Jung Kim, Se-Yun Jang, Moo Hyun Oh, Duck-Hyung Lee, Ki Churl Chang
Obesity-associated non-alcoholic fatty liver disease (NAFLD) increases coagulation and inflammation. We hypothesized that (S)YS-51, an agent found to be beneficial in animal models of sepsis, may reduce NAFLD in high-fat diet (HFD) mice by reducing coagulation and inflammation. C57BL/6 mice were fed either a chow diet or HFD and each was supplemented with or without (S)YS-51 (10mg/kg, daily, i.p.) for 16 weeks. The results showed that HFD caused significant increases in lipogenesis [CD36, fatty acid synthase (FAS) and sterol response element binding protein (SREBP)-1c mRNA and protein], inflammation [monocyte chemotactic protein (MCP)-1, tumor necrosis factor (TNF)-α, intercellular cell adhesion molecule-1 (ICAM-1), TGF-β, and procollagen type 1 mRNA, macrophage infiltration] and coagulation [tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) mRNA and thrombin antithrombin complex (TAT)] in the liver, adipose tissue and serum, which were significantly reduced by (S)YS-51...
October 5, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27321153/a-quantitative-metabolomics-study-of-high-sodium-response-in-clostridium-acetobutylicum-atcc-824-acetone-butanol-ethanol-abe-fermentation
#13
Xinhe Zhao, Stefan Condruz, Jingkui Chen, Mario Jolicoeur
Hemicellulose hydrolysates, sugar-rich feedstocks used in biobutanol refinery, are normally obtained by adding sodium hydroxide in the hydrolyze process. However, the resulting high sodium concentration in the hydrolysate inhibits ABE (acetone-butanol-ethanol) fermentation, and thus limits the use of these low-cost feedstocks. We have thus studied the effect of high sodium on the metabolic behavior of Clostridium acetobutyricum ATCC 824, with xylose as the carbon source. At a threshold sodium concentration of 200 mM, a decrease of the maximum cell dry weight (-19...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27260465/a-conjugated-fatty-acid-present-at-high-levels-in-bitter-melon-seed-favorably-affects-lipid-metabolism-in-hepatocytes-by-increasing-nad-nadh-ratio-and-activating-ppar%C3%AE-ampk-and-sirt1-signaling-pathway
#14
Gou-Chun Chen, Hui-Min Su, Yu-Shun Lin, Po-Yen Tsou, Jong-Ho Chyuan, Pei-Min Chao
α-Eleostearic acid (α-ESA), or the cis-9, trans-11, trans-13 isomer of conjugated linolenic acid, is a special fatty acid present at high levels in bitter melon seed oil. The aim of this study was to examine the effect of α-ESA on hepatic lipid metabolism. Using H4IIEC3 hepatoma cell line, we showed that α-ESA significantly lowered intracellular triglyceride accumulation compared to α-linolenic acid (LN), used as a fatty acid control, in a dose- and time-dependent manner. The effects of α-ESA on enzyme activities and mRNA profiles in H4IIEC3 cells suggested that enhanced fatty acid oxidation and lowered lipogenesis were involved in α-ESA-mediated triglyceride lowering effects...
July 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/27245326/increasing-the-production-of-r-3-hydroxybutyrate-in-recombinant-escherichia-coli-by-improved-cofactor-supply
#15
Mariel Perez-Zabaleta, Gustav Sjöberg, Mónica Guevara-Martínez, Johan Jarmander, Martin Gustavsson, Jorge Quillaguamán, Gen Larsson
BACKGROUND: In a recently discovered microorganism, Halomonas boliviensis, polyhydroxybutyrate production was extensive and in contrast to other PHB producers, contained a set of alleles for the enzymes of this pathway. Also the monomer, (R)-3-hydroxybutyrate (3HB), possesses features that are interesting for commercial production, in particular the synthesis of fine chemicals with chiral specificity. Production with a halophilic organism is however not without serious drawbacks, wherefore it was desirable to introduce the 3HB pathway into Escherichia coli...
2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27155796/effective-asymmetric-bioreduction-of-ethyl-4-chloro-3-oxobutanoate-to-ethyl-r-4-chloro-3-hydroxybutanoate-by-recombinant-e-coli-cczu-a13-in-bmim-pf6-hydrolyzate-media
#16
Yu-Cai He, Zhi-Cheng Tao, Jun-Hua Di, Liang Chen, Lin-Bing Zhang, Dan-Ping Zhang, Gang-Gang Chong, Feng Liu, Yun Ding, Chun-Xia Jiang, Cui-Luan Ma
It was the first report that the concentrated hydrolyzates from the enzymatic hydrolysis of dilute NaOH (3wt%)-soaking rice straw at 30°C was used to form [Bmim]PF6-hydrolyzate (50:50, v/v) media for bioconverting ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl (R)-4-chloro-3-hydroxybutanoate [(R)-CHBE] (>99% e.e.) with recombinant E. coli CCZU-A13. Compared with pure glucose, the hydrolyzates could promote both initial reaction rate and the intracellular NADH content. Furthermore, emulsifier OP-10 (20mM) was employed to improve the reductase activity...
August 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27098689/l-lactate-mediated-neuroprotection-against-glutamate-induced-excitotoxicity-requires-aralar-agc1
#17
Irene Llorente-Folch, Carlos B Rueda, Irene Pérez-Liébana, Jorgina Satrústegui, Beatriz Pardo
UNLABELLED: ARALAR/AGC1/Slc25a12, the aspartate-glutamate carrier from brain mitochondria, is the regulatory step in the malate-aspartate NADH shuttle, MAS. MAS is used to oxidize cytosolic NADH in mitochondria, a process required to maintain oxidative glucose utilization. The role of ARALAR was analyzed in two paradigms of glutamate-induced excitotoxicity in cortical neurons: glucose deprivation and acute glutamate stimulation. ARALAR deficiency did not aggravate glutamate-induced neuronal death in vitro, although glutamate-stimulated respiration was impaired...
April 20, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27064085/chitin-enhances-biocontrol-of-rhodotorula-mucilaginosa-to-postharvest-decay-of-peaches
#18
Hongyin Zhang, Qiya Yang, Lingling Ge, Guochao Zhang, Xiaoli Zhang, Xiaoyun Zhang
Biological control using microbial antagonists is a promising alternative approach to synthetic fungicides. However, effective biological control requires enhancing the consistency and efficacy of the antagonists used to control postharvest diseases. This study investigated the effect of chitin on the biocontrol efficacy of Rhodotorula mucilaginosa against blue mold and Rhizopus decay of peaches and on the protein expression profiles of R. mucilaginosa. The antagonistic activity of R. mucilaginosa harvested from the nutrient yeast dextrose broth (NYDB) with 0...
July 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27056473/overexpressions-of-xyla-and-xylb-in-klebsiella-pneumoniae-lead-to-enhanced-1-3-propanediol-production-by-cofermentation-of-glycerol-and-xylose
#19
Xinyao Lu, Xiaomeng Fu, Hong Zong, Bin Zhuge
1,3-Propanediol (1,3-PD) is a valuable platform compound. Many studies have shown that the supplement of NADH plays a key role in the bioproduction of 1,3-PD from Klebsiella pneumoniae. In this study, the xylA and xylB genes from Escherichia coli were overexpressed individually or simultaneously in K. pneumoniae to improve the production of 1,3-PD by cofermentation of glycerol and xylose. Compared with the parent strain, the xylose consumption was significantly increased by the introduction of these two genes...
July 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27048556/nicotinic-acid-supplementation-in-diet-favored-intramuscular-fat-deposition-and-lipid-metabolism-in-finishing-steers
#20
Zhu-Qing Yang, Lin-Bin Bao, Xiang-Hui Zhao, Can-Yu Wang, Shan Zhou, Lu-Hua Wen, Chuan-Bian Fu, Jian-Ming Gong, Ming-Ren Qu
Nicotinic acid (NA) acting as the precursor of NAD(+)/NADH and NADP(+)/NADPH, participates in many biochemical processes, e.g. lipid metabolism. The main purpose of this study was to investigate the effects of dietary NA on carcass traits, meat quality, blood metabolites, and fat deposition in Chinese crossbred finishing steers. Sixteen steers with the similar body weight and at the age of 24 months were randomly allocated into control group (feeding basal diet) and NA group (feeding basal diet + 1000 mg/kg NA)...
June 2016: Experimental Biology and Medicine
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