keyword
MENU ▼
Read by QxMD icon Read
search

bioanalytical

keyword
https://www.readbyqxmd.com/read/28636139/review-of-hplc-and-lc-ms-ms-assays-for-the-determination-of-various-non-steroidal-anti-androgens-nsaa-used-in-the-treatment-of-prostate-cancer
#1
REVIEW
P S Suresh, Nuggehally R Srinivas, Ramesh Mullangi
Prostate cancer is the most common cancer and one of the leading causes for cancer deaths in men. One of the commonly used approaches to treat metastatic prostate cancer was via first generation non-steroidal anti-androgens (NSAA) namely flutamide, nilutamide, bicalutamide and topilutamide. Most of the prostate cancer patients who are initially responsive develop a most aggressive form of disease called castration-resistant prostate cancer (CRPC). Second generation NSAA receptor antagonists (enzalutamide, apalutamide and darolutamide) are emerging as additional new options to treat CRPC...
June 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28628861/enhancement-in-recovery-of-drugs-with-high-protein-binding-efficiency-from-human-plasma-using-magnetic-nanoparticles
#2
Aniruddha Bhati, Rucha P Desai, C N Ramchand
In this paper, we propose an alternate method for bioanalytical extraction of drugs from human plasma samples using bare magnetic nanoparticles. The magnetic nanoparticles (MNPs) were used for deproteination of biological samples that further assist in extraction of plasma bound drugs for bioanalytical studies. The method uses basic solvents (ethanol, methanol, etc.) rather than the expensive and toxic solvents. The MNPs provide several advantages like avoiding the use of centrifuge machine, and making extraction time effective...
June 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28628730/comparative-study-on-ambient-ionization-methods-for-direct-analysis-of-navel-orange-tissues-by-mass-spectrometry
#3
H Zhang, A Bibi, H Lu, J Han, H Chen
It is of sustainable interest to improve the sensitivity and selectivity of the ionization process, especially for direct analysis of complex samples without matrix separation. Herein, four ambient ionization methods including desorption atmospheric pressure chemical ionization (DAPCI), heat-assisted desorption atmospheric pressure chemical ionization (heat-assisted DAPCI), microwave plasma torch (MPT), and internal extractive electrospray ionization (iEESI) were employed for comparative analysis of the navel orange tissue samples by mass spectrometry...
June 19, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28627276/comprehensive-graphical-presentation-of-data-from-incurred-sample-reanalysis
#4
Piotr J Rudzki, Przemysław Biecek, Michał Kaza
AIM: Incurred sample reanalysis (ISR) contributes to the reliability of pharmacokinetic studies. Despite regulatory guidelines having adopted ISR methodology, graphical presentation of data has been overlooked. MATERIALS & METHODS: Different graphs were tested for datasets including limited, standard and large numbers of ISR pairs. The datasets covered both passed and failed cases. RESULTS: We have developed a combination of complementary plots enabling the visual inspection of ISR data quality: %difference versus mean concentration and cumulative ISR plot...
June 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28623772/development-and-validation-of-a-highly-sensitive-lc-esi-ms-ms-method-for-estimation-of-iiim-mcd-211-a-novel-nitrofuranyl-methyl-piperazine-derivative-with-potential-activity-against-tuberculosis-application-to-drug-development
#5
Asmita Magotra, Anjna Sharma, Ajai Prakash Gupta, Priya Wazir, Shweta Sharma, Parvinder Pal Singh, Manoj Kumar Tikoo, Ram A Vishwakarma, Gurdarshan Singh, Utpal Nandi
In the present study, a simple, sensitive, specific and rapid liquid chromatography (LC) tandem mass spectrometry (MS/MS) method was developed and validated according to the Food and Drug Administration (FDA) guidelines for estimation of IIIM-MCD-211 (a potent oral candidate with promising action against tuberculosis) in mice plasma using carbamazepine as internal standard (IS). Bioanalytical method consisted of one step protein precipitation for sample preparation followed by quantitation in LC-MS/MS using positive electrospray ionization technique (ESI) operating in multiple reaction monitoring (MRM) mode...
June 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28621256/detection-and-analysis-of-microbes-bioanalytes-and-micropollutants-focusing-on-food-and-environmental-samples-using-nanoparticle-based-detection-systems-microfluidic-analytical-devices-and-related-techniques
#6
https://www.readbyqxmd.com/read/28617495/thiol-ene-micropillar-array-electrospray-ionization-platform-for-zeptomole-level-bioanalysis
#7
K Lipponen, S Tähkä, T Sikanen, V Jokinen, A Tatikonda, S Franssila, R Kostiainen, T Kotiaho
A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10(-20) mol) for a peptide standard...
June 15, 2017: Analyst
https://www.readbyqxmd.com/read/28617037/quantitative-determination-of-a-sirna-ad00370-in-rat-plasma-using-peptide-nucleic-acid-probe-and-hplc-with-fluorescence-detection
#8
Qingguo Tian, Juan Rogness, Min Meng, Zheng Li
AIM: Toxicokinetic and pharmacokinetic studies of therapeutic oligonucleotides require validated bioanalytical methods for sensitive and specific quantification of oligonucleotide drug candidates in biological samples. RESULTS: A peptide nucleic acid (PNA) hybridization-based HPLC-fluorescence assay was developed and validated for quantification of Arrowhead Pharmaceuticals' proprietary siRNA in rat plasma samples via hybridization and anion-exchange-HPLC (AEX-HPLC) with fluorescence detection...
June 15, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28616944/acquiring-multiple-signals-along-with-the-reaction-time-improving-recognition-capability-of-a-multidimensional-colorimetric-sensor-array-for-sensitive-protein-detection
#9
Jiaoe Yang, Liuying He, Yuexiang Lu, Xinxin Gao, Feiyang Wang, Wenjie Jing, Yueying Liu
The development of sensitive and cheap sensor arrays for identification of proteins plays an important role in many bioanalytical and clinical investigations. Here, we introduce a multidimensional colorimetric sensor array for the detection of multiple proteins based on acquiring multiple signals along with the reaction time to enhance the discrimination ability. In a single experiment, the unique fingerprint for each protein against the sensor array is generated from a response absorbance signal at three reaction time points (at 10 min, 15 min, and 20 min)...
June 15, 2017: Analyst
https://www.readbyqxmd.com/read/28604104/intranasal-delivery-of-quercetin-loaded-mucoadhesive-nanoemulsion-for-treatment-of-cerebral-ischaemia
#10
Niyaz Ahmad, Rizwan Ahmad, Atta Abbas Naqvi, Md Aftab Alam, Mohammad Ashafaq, Rehan Abdur Rub, Farhan Jalees Ahmad
BACKGROUND: Quercetin (QUR), as an antioxidant flavonoid, exhibits potential role in the amelioration of cerebral ischaemia; however, poor solubility as well as oral absorption results low serum and tissue levels for this drug. PURPOSE OF THE STUDY: To enhance bioavailability, this study aims to prepare QUR nanoemulsions and administer via non-invasive nasal route in order to evaluate the drug targeting in brain. METHODS: Quercetin mucoadhesive nanoemulsion (QMNE) was prepared (ionic gelation method) and optimized using various parameters, that is, particle size, entrapment efficiency, zeta potential and ex vivo permeation study...
June 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28603847/tight-molecular-recognition-of-benzo-a-pyrene-by-a-high-affinity-antibody
#11
Andreas Eichinger, Irmgard Neumaier, Michael Pschenitza, Reinhard Nießner, Dietmar Knopp, Arne Skerra
Benzo[a]pyrene, produced during the combustion of organic material, is an abundant noxious pollutant because of its carcinogenic metabolic degradation products. The high affinity (KD ≈ 3 nM) monoclonal antibody 22F12 allows facile bioanalytical quantification of benzo[a]pyrene even in complex matrices. Here, we report its functional and X-ray crystallographic analysis in complex with 3-hydroxybenzo[a]pyrene after cloning of the V-genes and production as a recombinant Fab fragment. The polycyclic aromatic hydrocarbon is bound in a deep pocket beween the light and heavy chains surrounded mainly by aromatic and aliphatic amino acid side chains...
June 11, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28602609/satellite-rats-are-redundant-in-embryo-fetal-development-studies
#12
Manon Beekhuijzen, Yaomi de Lange, Ankie Lambregts, Birgit Peter, Mira Wenker, Harry Emmen
Routinely in many laboratories, satellite rats are added to embryo-fetal development (EFD) studies for pharmaceuticals to assess toxicokinetic (TK) properties, because it is assumed that collection of multiple blood samples with relatively large volumes might affect the study outcome. With recent refinement of blood sampling techniques, this belief requires reevaluation. The current work showed successful implementation of jugular vein blood sampling in an EFD rat study without satellite animals, thereby reducing the number of rats in standard EFD studies for pharmaceuticals by 20%...
June 7, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28595106/development-and-validation-of-a-bioanalytical-method-based-on-lc-ms-ms-analysis-for-the-quantitation-of-cigb-814-peptide-in-plasma-from-rheumatoid-arthritis-patients
#13
Ania Cabrales-Rico, Yassel Ramos, Vladimir Besada, María Del Carmen Domínguez, Noraylis Lorenzo, Osmel García, Jorge Alexis, Dinhora Prada, Yusimí Reyes, Ana Marta López, Yordanka Masforrol, Hilda Garay, Luis Javier González
CIGB-814, originally named as E18-3 APL1 or APL1 in preclinical experiments, is a novel therapeutic peptide candidate for Rheumatoid Arthritis (RA). It is an altered peptide ligand containing a novel CD4+ T-cell epitope of human heat shock protein 60 (83-109, MW 2988.38g/mol) with a mutation (D(100)→L) that increases its affinity for HLA-II type molecules associated to RA. A bioanalytical method, based on LC-MS/MS analysis, in the SRM mode was developed and fully validated to quantify this peptide in human plasma...
June 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28589510/singlicate-ligand-binding-assay-using-an-automated-microfluidic-system-a-clinical-case-study
#14
Hao Jiang, Alex Kozhich, Jennifer Cummings, Janice Gambardella, Frank Zambito, Craig Titsch, Jonathan Haulenbeek, Kelli Phillips, Ross Fergus, Heather Myler
The bioanalytical strategy for monoclonal antibody therapeutics, intended for multiple oncology indications, includes multiple integrated measurements of pharmacologically relevant therapeutics from discovery through development. Three ligand binding assays were cohesively developed and validated, as applicable, using the Gyrolab microfluidic system for the measurement of a free monoclonal antibody BMS-986207. Accuracy and precision demonstrate %bias from -6.3 to 4.4%, percent coefficient of variation (%CV) from 2...
June 6, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28578848/study-on-pharmacokinetics-and-bioequivalence-of-vonoprazan-pyroglutamate-in-rats-by-liquid-chromatography-with-tandem-mass-spectrometry
#15
Yuan Qiao, Jun Zhao, Xuanfeng Yue, Yang Zhang, Ruitao Zhang, Yuan Xu, Xiuling Tang, Xueying Liu, Qingwei Wang
Vonoprazan Fumarate (TAK-438F) is a new and effective drug approved in Japan in 2014 for treatment and prevention of acid-related diseases (ARDs), which exhibits many advantages compared with traditional proton-pump inhibitors (PPIs). However, the clinical applications of TAK0-438F suffers limitation due to the lack of injection dosage form. Efforts to overcome this limitation lead to the systhesis of Vonoprazan pyroglutamate (TAK-438P) for its high water solubility and more potent antisecretory effect. This was the first report to establish and validate a reliable and sensitive LC-MS/MS method for the quantification of TAK-438P in rat plasma and tissues (heart, liver, spleen, liver, kidney, rain, stomach and small intestine)...
August 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28578404/a-simple-and-rapid-lc-ms-ms-method-for-therapeutic-drug-monitoring-of-cetuximab-a-gpco-unicancer-proof-of-concept-study-in-head-and-neck-cancer-patients
#16
François Becher, Joseph Ciccolini, Diane-Charlotte Imbs, Clémence Marin, Claire Fournel, Charlotte Dupuis, Nicolas Fakhry, Bertrand Pourroy, Aurélie Ghettas, Alain Pruvost, Christophe Junot, Florence Duffaud, Bruno Lacarelle, Sebastien Salas
Administration of first-in-class anti-EGFR monoclonal antibody cetuximab is contingent upon extensive pharmacogenomic testing. However in addition to tumor genomics, drug exposure levels could play a critical, yet largely underestimated role, because several reports have demonstrated that cetuximab pharmacokinetic parameters, in particular clearance values, were associated with survival in patients. Here, we have developed an original bioanalytical method based upon the use of LC-MS/MS technology and a simplified sample preparation procedure to assay cetuximab in plasma samples from patients, thus meeting the requirements of standard Therapeutic Drug Monitoring in routine clinical practice...
June 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28577486/an-accurate-and-robust-lc-ms-ms-method-for-the-quantification-of-chlorfenvinphos-ethion-and-linuron-in-liver-samples
#17
Ali Kadar, Ludovic Peyre, Georges de Souza, Henri Wortham, Pierre Doumenq, Roger Rahmani
A method for the determination of chlorfenvinphos, ethion and linuron in liver samples by LC-MS/MS is described. Sample treatment was performed by using Sola™ polymeric reverse phase SPE cartridges after protein precipitation. Gradient elution using 10 mM ammonium formate in methanol (A) and 10 mM ammonium formate in water (B) was used for chromatographic separation of analytes on a Hypersil™ end-capped Gold PFP reverse phase column (100 mm × 2.1 mm, 3 μm). All analytes were quantified without interference, in positive ionization mode using multiple reaction monitoring (MRM) with chlorfenvinphos-d10 as internal standard...
May 29, 2017: Chemosphere
https://www.readbyqxmd.com/read/28571028/integration-of-the-forensic-dimension-into-anti-doping-strategies
#18
François Marclay, Martial Saugy
Traditionally, research in anti-doping has been stimulated by the need for technological improvements to accommodate the expansion of the list of prohibited substances and methods. Nevertheless, in recent years, anti-doping found itself at a crossroads due to the increasing complexity and constant refinement of doping methods. As illustrated by the 2012 USADA (United States Anti-Doping Agency) versus Lance Armstrong case, a change in paradigm was necessary. The exploration of new scientific avenues to understand the mechanisms of doping and pinpoint its practice was most needed to allow designing more efficient preventive or disruptive strategies...
2017: Medicine and Sport Science
https://www.readbyqxmd.com/read/28567056/rapid-determination-of-isomeric-benzoylpaeoniflorin-and-benzoylalbiflorin-in-rat-plasma-by-lc-ms-ms-method
#19
Chuanqi Zhou, Xiaoke Wang
Benzoylpaeoniflorin (BP) is a potential therapeutic agent against oxidative stress related Alzheimer's disease. In this study, a more rapid, selective, and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to determine BP in rat plasma distinguishing with a monoterpene isomer, benzoylalbiflorin (BA). The method showed a linear response from 1 to 1000 ng/mL (r > 0.9950). The precision of the interday and intraday ranged from 2.03 to 12.48% and the accuracy values ranged from -8...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28565916/ubiquitin-immobilized-on-mesoporous-mcm41-silica-surfaces-analysis-by-solid-state-nmr-with-biophysical-and-surface-characterization
#20
Nurit Adiram-Filiba, Avital Schremer, Eli Ohaion, Merav Nadav-Tsubery, Tammi Lublin-Tennenbaum, Keren Keinan-Adamsky, Gil Goobes
Deriving the conformation of adsorbed proteins is important in the assessment of their functional activity when immobilized. This has particularly important bearings on the design of contemporary and new encapsulated enzyme-based drugs, biosensors, and other bioanalytical devices. Solid-state nuclear magnetic resonance (NMR) measurements can expand our molecular view of proteins in this state and of the molecular interactions governing protein immobilization on popular biocompatible surfaces such as silica...
May 31, 2017: Biointerphases
keyword
keyword
88645
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"