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https://www.readbyqxmd.com/read/28934870/a-bioanalytical-approach-of-chemical-composition-bioactivity-and-cytotoxicity-of-berteroa-incana-l-herb
#1
Oana Ungureanu, Florentina Gatea, Ana Maria Seciu, Eugenia Dumitra Teodor, Isabela Madalina Nicorescu, Gabriel Lucian Radu
Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects. Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect. The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4...
September 21, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28931321/interstitial-glucose-and-lactate-levels-are-inversely-correlated-with-the-body-mass-index-need-for-in-vivo-calibration-of-glucose-sensor-results-with-blood-values-in-obese-patients
#2
Barbara Enderle, Isabella Moser, Cecil Kannan, Karl Otfried Schwab, Gerald Urban
BACKGROUND: Continuously measured glucose and lactate levels in interstitial fluid (ISF) may markedly differ from their respective blood levels. METHODS: Combining microdialysis with a bioanalytical microsystem, the interstitial glucose and lactate concentrations of eight male volunteers with different body mass index (BMI) were monitored during a 2-fold glucose tolerance test over the period of three hours. RESULTS: Significant correlations were found between abdominally measured sensor results and reference measurements ( R(2) = ...
September 1, 2017: Journal of Diabetes Science and Technology
https://www.readbyqxmd.com/read/28930438/supported-planar-mammalian-membranes-as-models-of-in-vivo-cell-surface-architectures
#3
Han-Yuan Liu, Hannah Grant, Hung-Lun Hsu, Raya Sorkin, Filip Bošković, G J L Wuite, Susan Daniel
Emerging technologies use cell plasma membrane vesicles or 'blebs' as an intermediate to form molecularly complete, planar cell surface mimetics that are compatible with a variety of characterization tools and microscopy methods. This approach enables direct incorporation of membrane proteins into supported lipid bilayers without using detergents and reconstitution and preserves native lipids and membrane species. Such a system can be advantageous as in vitro models of in vivo cell surfaces for study of the roles of membrane proteins as drug targets in drug delivery, host-pathogen interactions, tissue engineering, and many other bioanalytical and sensing applications...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28926208/ionic-liquids-solvents-and-sorbents-in-sample-preparation
#4
REVIEW
Kevin D Clark, Miranda N Emaus, Marcelino Varona, Ashley N Bowers, Jared L Anderson
The applications of ionic liquids (ILs) and IL-derived sorbents are rapidly expanding. By careful selection of the cation and anion components, the physicochemical properties of ILs can be altered to meet the requirements of specific applications. Reports of IL solvents possessing high selectivity for specific analytes are numerous and continue to motivate the development of new IL-based sample preparation methods that are faster, more selective, and environmentally benign compared to conventional organic solvents...
September 19, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28920457/implementing-a-tiered-approach-to-bioanalytical-method-validation-for-large-molecule-ligand-binding-assay-methods-in-pharmacokinetic-assessments
#5
Rebecca G Watson, Adrienne Clements-Egan, Allen Schantz, Mark Ware, Bonnie Wu, Tong-Yuan Yang, Gopi Shankar, Joseph C Marini
Bioanalytical methods must enable the delivery of data that meet sound, scientifically justified, fit-for-purpose criteria. At early phases of biotherapeutic drug development, suitable criteria of a ligand-binding assay could be met for pharmacokinetic (PK) in-study sample testing without a full validation defined by regulatory guidelines. To ensure fit-for-purpose methods support PK testing through all phases of biotherapeutic development, three tiers of method validation - regulatory, scientific and research validations - are proposed...
September 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28920452/challenges-and-opportunities-in-bioanalytical-support-for-gene-therapy-medicinal-product-development
#6
Mark Ma, Nanda Balasubramanian, Robert Dodge, Yan Zhang
Gene and nucleic acid therapies have demonstrated patient benefits to address unmet medical needs. Beside considerations regarding the biological nature of the gene therapy, the quality of bioanalytical methods plays an important role in ensuring the success of these novel therapies. Inconsistent approaches among bioanalytical labs during preclinical and clinical phases have been observed. There are many underlying reasons for this inconsistency. Various platforms and reagents used in quantitative methods, lacking of detailed regulatory guidance on method validation and uncertainty of immunogenicity strategy in supporting gene therapy may all be influential...
September 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28920451/demonstrating-biosimilar-and-originator-antidrug-antibody-binding-comparability-in-antidrug-antibody-assays-a-practical-approach
#7
Janka Ryding, Michael Stahl, Martin Ullmann
Biosimilar drug development has brought new challenges to bioanalytical ligand-binding assays used to determine drug concentration, antidrug antibodies and neutralizing antibodies. One particular challenge is how to demonstrate that the antidrug antibody assay can adequately detect antibodies against both biosimilar and originator. In this paper, we review the current guidelines and literature for practical recommendations and present a gap analysis. Case examples of antibody binding comparability testing are presented, and the challenges and implications are discussed...
September 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28918323/quantitative-determination-of-carfilzomib-in-mouse-plasma-by-liquid-chromatography-tandem-mass-spectrometry-and-its-application-to-a-pharmacokinetic-study
#8
Jee Sun Min, Jiseon Kim, Jung Ho Kim, Doyun Kim, Yu Fen Zheng, Ji Eun Park, Wooin Lee, Soo Kyung Bae
A highly sensitive and rapid LC-MS/MS method was developed and validated to determine the levels of carfilzomib in mice plasma by using chlorpropamide as an internal standard. Carfilzomib and chlorpropamide were extracted from 5 μL of plasma after protein precipitation with acetonitrile. Chromatographic separation was performed on Phenomenex Luna C18 column (50×2.0mm id, 3μm). The mobile phase consisted of 0.1% formic acid in acetonitrile -0.1% formic acid in water (1:1v/v) and the flow rate was 0.3mL/min...
September 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28915419/sensitivity-improvement-of-the-lc-ms-ms-quantification-of-carbidopa-in-human-plasma-and-urine-by-derivatization-with-2-4-pentanedione
#9
Nico C van de Merbel, Kees J Bronsema, Steven H Gorman, Ray Bakhtiar
The reliable quantification of carbidopa in biological samples at low concentrations is challenging because of the polar and highly unstable nature of the compound. In this paper, LC-MS/MS methods are described for the determination of carbidopa in 50μL of human plasma and 25μL of human urine in the concentration ranges 1-1,000ng/mL and 100-50,000ng/mL, respectively. After a simple protein precipitation (plasma) or dilution (urine) step, carbidopa is derivatized at its hydrazine moiety by reaction for one hour with 2,4-pentanedione under acidic conditions and at 40°C...
September 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28913580/in-vitro-tools-for-the-toxicological-evaluation-of-sediments-and-dredged-materials-intra-and-inter-laboratory-comparisons-of-chemical-and-bioanalytical-methods
#10
Kathrin Eichbaum, Markus Brinkmann, Leonie Nuesser, Carolin Gembé, Marina Ohlig, Sebastian Buchinger, Georg Reifferscheid, Markus Hecker, John P Giesy, Henner Hollert
The implementation of in vitro bioassays for the screening of dioxin-like compounds (DLCs) into management guidelines of dredged material is of increasing interest to regulators and risk assessors. This study reports on an intra- and inter-laboratory comparison study between four independent laboratories. A bioassay battery consisting of RTL-W1 (7-ethoxy-resorufin-O-deethylase; EROD), H4IIE (micro-EROD), and H4IIE-luc cells was used to assess aryl hydrocarbon receptor-mediated effects of sediments from two major European rivers, differently contaminated with DLCs...
September 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28911662/pharmacokinetic-interactions-of-herbal-medicines-for-the-treatment-of-chronic-hepatitis
#11
REVIEW
Tun-Pin Hsueh, Wan-Ling Lin, Tung-Hu Tsai
Chronic liver disease is a serious global health problem, and an increasing number of patients are seeking alternative medicines or complementary treatment. Herbal medicines account for 16.8% of patients with chronic liver disease who use complementary and alternative therapies. A survey of the National Health Insurance Research Database in Taiwan reported that Long-Dan-Xie-Gan-Tang, Jia-Wei-Xia-Yao-San, and Xiao-Chai-Hu-Tang (Sho-saiko-to) were the most frequent formula prescriptions for chronic hepatitis used by traditional Chinese medicine physicians...
April 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28903089/simultaneous-determination-of-pentoxifylline-metabolites-m1-lisofylline-m4-and-m5-and-caffeine-in-plasma-and-dried-blood-spots-for-pharmacokinetic-studies-in-preterm-infants-and-neonates
#12
Madhu Page-Sharp, Tobias Strunk, Sam Salman, Julie Hibbert, Sanjay K Patole, Laurens Manning, Kevin T Batty
Advances in bioanalytical methods are facilitating micro-volume and dried blood spot (DBS) analysis of drugs in biological matrices for pharmacokinetic studies in children and neonates. We sought to develop a UPLC-MS/MS assay for simultaneous measurement of caffeine, pentoxifylline (PTX) and three metabolites of PTX in both plasma and DBS. Caffeine, PTX, the metabolites M1 (lisofylline), M4 and M5, and the internal standards (caffeine-d9 and PTX-d6) were separated using a Waters Aquity T3 UPLC C18 column and gradient mobile phase (water-methanol-formic acid)...
August 26, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28901171/recent-china-food-and-drug-administration-reform-impact-on-the-present-and-future-of-bioanalytical-contract-research-organization-laboratories-in-china
#13
https://www.readbyqxmd.com/read/28894966/targeting-cancer-specific-glycans-by-cyclic-peptide-lectinomimics
#14
Maria C Rodriguez, Austin B Yongye, Mihael Cudic, Karina Martinez Mayorga, Enbo Liu, Barbara M Mueller, Jon Ainsley, Tatyana Karabencheva-Christova, Christo Z Christov, Mare Cudic, Predrag Cudic
The transformation from normal to malignant phenotype in human cancers is associated with aberrant cell-surface glycosylation. Thus, targeting glycosylation changes in cancer is likely to provide not only better insight into the roles of carbohydrates in biological systems, but also facilitate the development of new molecular probes for bioanalytical and biomedical applications. In the reported study, we have synthesized lectinomimics based on odorranalectin 1; the smallest lectin-like cyclic peptide isolated from the frog Odorrana grahami skin, and assessed the ability of these peptides to bind specific carbohydrates on molecular and cellular levels...
September 11, 2017: Amino Acids
https://www.readbyqxmd.com/read/28894622/a-liquid-chromatography-tandem-mass-spectrometry-method-for-evaluation-of-two-brands-of-enalapril-20%C3%A2-mg-tablets-in-healthy-human-volunteers
#15
Wael Abu Dayyih, Mohammed Hamad, Ahmad Abu Awwad, Eyad Mallah, Zainab Zakarya, Alice Abu Dayyih, Tawfiq Arafat
Enalapril is an angiotensin-converting enzyme inhibitor used for treatment of hypertension and chronic heart disease. Enalaprilat is its active metabolite responsible for the activity. This study aimed to develop and validate a method for enalapril and enalaprilat analysis and to determine the bioequivalence of two tablet formulae of enalapril. LC-MS/MS bioanalytical method was developed and validated and then applied to evaluate the bioavailability of two enalapril formulae. Antihyperglycemic sitagliptin was used as internal standard (IS)...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28888173/lc-ms-ms-assay-for-the-quantitation-of-the-atr-kinase-inhibitor-vx-970-in-human-plasma
#16
Brian F Kiesel, Jonas Scemama, Robert A Parise, Liza Villaruz, Andre Iffland, Austin Doyle, Percy Ivy, Edward Chu, Christopher J Bakkenist, Jan H Beumer
DNA damaging chemotherapy and radiation are widely used standard-of-care modalities for the treatment of cancer. Nevertheless, the outcome for many patients remains poor and this may be attributed, at least in part, to highly effective DNA repair mechanisms. Ataxia-telangiectasia mutated and Rad3-related (ATR) is a key regulator of the DNA-damage response (DDR) that orchestrates the repair of damaged replication forks. ATR is a serine/threonine protein kinase and ATR kinase inhibitors potentiate chemotherapy and radiation...
August 31, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28886522/quantification-of-the-antimalarial-drug-pyronaridine-in-whole-blood-using-lc-ms-ms-increased-sensitivity-resulting-from-reduced-non-specific-binding
#17
Daniel Blessborn, Karnrawee Kaewkhao, Lijiang Song, Nicholas J White, Nicholas P J Day, Joel Tarning
Malaria is one of the most important parasitic diseases of man. The development of drug resistance in malaria parasites is an inevitable consequence of their widespread and often unregulated use. There is an urgent need for new and effective drugs. Pyronaridine is a known antimalarial drug that has received renewed interest as a partner drug in artemisinin-based combination therapy. To study its pharmacokinetic properties, particularly in field settings, it is necessary to develop and validate a robust, highly sensitive and accurate bioanalytical method for drug measurements in biological samples...
August 26, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28881312/lc-ms-ms-assay-for-the-quantitation-of-the-ribonucleotide-reductase-inhibitor-triapine-in-human-plasma
#18
Julia Matsumoto, Brian F Kiesel, Robert A Parise, Jianxia Guo, Sarah Taylor, Marilyn Huang, Julie L Eiseman, S Percy Ivy, Charles Kunos, Edward Chu, Jan H Beumer
The ribonucleotide reductase inhibitor and radiosensitizer triapine (3-aminopyridine-2-carboxaldehyde thiosemicarbazone (3-AP), NSC 663249) is clinically being evaluated via the intravenous (IV) route for the treatment of cervical and vulvar cancer in combination with primary cisplatin chemoradiation. The need for a 2-h infusion and frequent administration of triapine is logistically challenging, prompting us to pursue oral (PO) administration. In support of the clinical trial investigating oral triapine in combination with chemoradiation, we developed and validated a novel LC-MS/MS assay for the quantification of triapine in 50μL human plasma...
August 31, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28881273/investigating-the-androgenic-activity-of-ozonation-transformation-products-of-testosterone-and-androstenedione
#19
Paul Westlund, Siavash Isazadeh, Viviane Yargeau
This study investigates the impact of ozonation on the concentration of two androgen steroid hormones, testosterone and androstenedione, and on androgenic activity as measured using the YAS assay. While these compounds are remarkably similar in chemical structure and showed comparable removal profiles in the presence and absence of a hydroxyl scavenger (isopropanol), large differences between percent removal of target compounds using chemical analysis and percent removal of bioactivity was observed when small ozone doses were applied...
August 18, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28880034/quantum-dots-from-fluorescence-to-chemiluminescence-bioluminescence-electrochemiluminescence-and-electrochemistry
#20
REVIEW
Jun Yao, Lin Li, Pingfan Li, Mei Yang
During the past decade, nanotechnology has become one of the major forces driving basic and applied research. As a novel class of inorganic fluorochromes, research into quantum dots (QDs) has become one of the fastest growing fields of nanotechnology today. QDs are made of a semiconductor material with tunable physical dimensions as well as unique optoelectronic properties, and have attracted multidisciplinary research efforts to further their potential bioanalytical applications. Recently, numerous optical properties of QDs, such as narrow emission band peaks, broad absorption spectra, intense signals, and remarkable resistance to photobleaching, have made them biocompatible and sensitive for biological assays...
September 21, 2017: Nanoscale
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