keyword
MENU ▼
Read by QxMD icon Read
search

bioanalytical

keyword
https://www.readbyqxmd.com/read/28231575/individual-biomarkers-using-molecular-personalized-medicine-approaches
#1
Hans P Zenner
Molecular personalized medicine tries to generate individual predictive biomarkers to assist doctors in their decision making. These are thought to improve the efficacy and lower the toxicity of a treatment. The molecular basis of the desired high-precision prediction is modern "omex" technologies providing high-throughput bioanalytical methods. These include genomics and epigenomics, transcriptomics, proteomics, metabolomics, microbiomics, imaging, and functional analyses. In most cases, producing big data also requires a complex biomathematical analysis...
February 24, 2017: ORL; Journal for Oto-rhino-laryngology and its related Specialties
https://www.readbyqxmd.com/read/28223873/development-and-validation-of-bioanalytical-uhplc-uv-method-for-simultaneous-analysis-of-unchanged-fenofibrate-and-its-metabolite-fenofibric-acid-in-rat-plasma-application-to-pharmacokinetics
#2
Rayan G Alamri, Kazi Mohsin, Ajaz Ahmad, Mohammad Raish, Fars K Alanazi
A simple, precise, selective and fast ultra-high performance liquid chromatography (UHPLC-UV) method has been developed and validated for the simultaneous determination of a lipid regulating agent fenofibrate and its metabolite fenofibric acid in rat plasma. The chromatographic separation was carried out on a reversed-phase Acquity® BEH C18 column using methanol-water (65:35, v/v) as the mobile phase. The isocratic flow was 0.3 ml/min with rapid run time of 2.5 min and UV detection was at 284 nm. The method was validated over a concentration range of 100-10000 ng/ml (r(2) ⩾ 0...
January 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28220712/a-novel-quantitative-method-for-recovering-precipitated-uric-acid-in-urine-and-analysis-by-lc-ms-ms
#3
Lifang Sun, Dongmei Zhou, David M Wilson
AIM: Precipitation of uric acid (UA) in human urine had caused poor recovery and underestimation of UA in study samples. RESULTS: A UA recovery method was developed for analyzing frozen urine samples that potentially contain precipitated UA. Samples were treated with 3% v/v of a solution containing 8 M potassium hydroxide and 20% v/v of ammonium thioglycolate. The high pH enables rapid dissolution of UA crystals, while the thioglycolate protects UA from oxidation at high pH...
March 2017: Bioanalysis
https://www.readbyqxmd.com/read/28219796/simultaneous-quantitation-of-abiraterone-enzalutamide-n-desmethyl-enzalutamide-and-bicalutamide-in-human-plasma-by-lc-ms-ms
#4
Kyu-Pyo Kim, Robert A Parise, Julianne L Holleran, Lionel D Lewis, Leonard Appleman, Nielka van Erp, Michael J Morris, Jan H Beumer
Inhibiting the androgen receptor (AR) pathway is an important clinical strategy in metastatic prostate cancer. Novel agents including abiraterone acetate and enzalutamide have been shown to prolong life in men with metastatic, castration-resistant prostate cancer (mCRPC). To evaluate the pharmacokinetics of AR-targeted agents, we developed and validated an LC-MS/MS assay for the quantitation of enzalutamide, N-desmethyl enzalutamide, abiraterone and bicalutamide in 0.05mL human plasma. After protein precipitation, chromatographic separation was achieved with a Phenomenex Synergi Polar-RP column and a linear gradient of 0...
February 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28213176/lc-ms-ms-assay-for-the-simultaneous-quantitation-of-the-atm-inhibitor-az31-and-the-atr-inhibitor-azd6738-in-mouse-plasma
#5
Brian F Kiesel, Jeffrey C Shogan, Madani Rachid, Robert A Parise, Frank P Vendetti, Christopher J Bakkenist, Jan H Beumer
The ATM kinase inhibitor AZ31 and ATR kinase inhibitor AZD6738 are in various phases of preclinical and clinical evaluation for their ability to potentiate chemoradiation. To support the preclinical evaluation of their pharmacokinetics, we developed and validated an LC-MS/MS assay for the simultaneous quantification of AZ31 and AZD6738 in mouse plasma. A "dilute and shoot" method was used to precipitate proteins from a sample volume of 50μL. Chromatographic separation was achieved using a Phenomenex Polar-RP column and a gradient mobile phase consisting of methanol-water with 0...
February 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28211898/rapid-prototyping-of-all-solution-processed-multi-lengthscale-electrodes-using-polymer-induced-thin-film-wrinkling
#6
Christine M Gabardo, Robert C Adams-McGavin, Barnabas C Fung, Eric J Mahoney, Qiyin Fang, Leyla Soleymani
Three-dimensional electrodes that are controllable over multiple lengthscales are very important for use in bioanalytical systems that integrate solid-phase devices with solution-phase samples. Here we present a fabrication method based on all-solution-processing and thin film wrinkling using smart polymers that is ideal for rapid prototyping of tunable three-dimensional electrodes and is extendable to large volume manufacturing. Although all-solution-processing is an attractive alternative to vapor-based techniques for low-cost manufacturing of electrodes, it often results in films suffering from low conductivity and poor substrate adhesion...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210984/effect-of-solution-ionic-strength-on-the-pka-of-the-nitroxide-ph-epr-probe-2-2-3-4-5-5-hexamethylimidazolidin-1-oxyl
#7
Kaleigh Margita, Maxim A Voinov, Alex I Smirnov
Spin probe and spin labeling Electron Paramagnetic Resonance methods are indispensable research tools for solving a wide range of bioanalytical problems-from measuring microviscosity and polarity of phase-separated liquids to oxygen concentrations in tissues. One of the emerging uses of spin probes are the studies of proton transfer-related and surface electrostatic phenomena. The latter Electron Paramagnetic Resonance methods rely on molecular probes containing an additional functionality capable of reversible ionization (protonation, in particular) in the immediate proximity to an Electron Paramagnetic Resonance-active reporter group, such as (N-O(•)) for nitroxides...
February 17, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28199895/a-rapid-and-robust-uhplc-dad-method-for-the-quantification-of-amphotericin-b-in-human-plasma
#8
Sebastiano Barco, Alessia Zunino, Antonio D'Avolio, Laura Barbagallo, Angelo Maffia, Gino Tripodi, Elio Castagnola, Giuliana Cangemi
Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency...
February 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28198806/arrays-of-individual-dna-molecules-on-nanopatterned-substrates
#9
Roland Hager, Alma Halilovic, Jonathan R Burns, Friedrich Schäffler, Stefan Howorka
Arrays of individual molecules can combine the advantages of microarrays and single-molecule studies. They miniaturize assays to reduce sample and reagent consumption and increase throughput, and additionally uncover static and dynamic heterogeneity usually masked in molecular ensembles. However, realizing single-DNA arrays must tackle the challenge of capturing structurally highly dynamic strands onto defined substrate positions. Here, we create single-molecule arrays by electrostatically adhering single-stranded DNA of gene-like length onto positively charged carbon nanoislands...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28193072/semiautomated-support-photoelectrochemical-immunosensing-platform-for-portable-and-high-throughput-immunoassay-based-on-au-nanocrystal-decorated-specific-crystal-facets-bivo4-photoanode
#10
Jian Shu, Zhenli Qiu, Zhenzhen Lin, Guoneng Cai, Huanghao Yang, Dianping Tang
Photoelectrochemical (PEC) measurement has been developed rapidly for bioanalysis in recent years. However, the actual application for most existed PEC bioanalytical systems is still a challenge because the perfect solutions for sensing surface design, high-throughput detection, and portability are lacked. To successfully overcome these limitations and realize accurate, continuous screening and assessing on prognostic indicator of early stage cancer on the spot, an innovative and portable semiautomated support power-free photoelectrochemical (SP-PEC) immunosensing platform consisted with a miniature semiautomatic injection system and digital multimeter (DMM) readout is designed (prostate specific antigen, PSA, was used as the proof-of-concept analyte)...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28192952/how-actuated-particles-effectively-capture-biomolecular-targets
#11
Alexander van Reenen, Arthur M de Jong, Menno W J Prins
Particles are widely used in bioanalytical methods to capture molecular targets, because of their high surface-to-volume ratio and adaptable surface functionalization. In this paper, a comprehensive study is reported of the effectiveness of protein capture by actuated magnetic particles. Association rate constants are quantified in experiments as well as in Brownian dynamics simulations, for different particle actuation configurations. The data reveal how the association rate depends on the particle velocity, the particle density, and particle assembly characteristics...
February 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192911/open-port-probe-sampling-interface-for-the-direct-coupling-of-biocompatible-solid-phase-microextraction-to-atmospheric-pressure-ionization-mass-spectrometry
#12
Germán Augusto Gómez-Ríos, Chang Liu, Marcos Tascon, Nathaly Reyes-Garcés, Don W Arnold, Thomas R Covey, Janusz Pawliszyn
In recent years, the direct coupling of solid phase microextraction (SPME) and mass spectrometry (MS) has shown its great potential to improve limits of quantitation, accelerate analysis throughput, and diminish potential matrix effects when compared to direct injection to MS. In this study, we introduce the open port probe (OPP) as a robust interface to couple biocompatible SPME (Bio-SPME) fibers to MS systems for direct electrospray ionization. The presented design consisted of minimal alterations to the front-end of the instrument and provided better sensitivity, simplicity, speed, wider compound coverage, and high-throughput in comparison to the LC-MS based approach...
February 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192759/precise-simultaneous-quantification-of-methadone-and-cocaine-in-rat-serum-and-brain-tissue-samples-following-their-successive-i-p-administration
#13
David S Nakhla, Lobna A Hussein, N Magdy, Inas A Abdallah, Hazem E Hassan
A sensitive high-performance liquid chromatography (HPLC) assay with dual UV detection has been developed and validated for the simultaneous quantification of methadone and cocaine in rat serum and brain tissue samples. Liquid-liquid extraction using hexanes was applied for samples extraction with Levo-Tetrahydropalmatine (L-THP) as the internal standard. Chromatographic separation of the analytes was achieved on a reversed-phase Waters Symmetry(®) C18 column (150mm×4.6mm, 5μm). A gradient elution was employed with a mobile phase consisting of 5mM potassium phosphate containing 0...
February 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28188298/comparison-between-radioanalysis-and-19-f-nuclear-magnetic-resonance-spectroscopy-in-the-determination-of-mass-balance-metabolism-and-distribution-of-pefloxacin
#14
Haitao Hu, Kishore Kumar Katyayan, Boris A Czeskis, Everett J Perkins, Palaniappan Kulanthaivel
Mass balance and metabolism studies using radiolabeled substances are well recognized as an important part of the drug development process. Herein, we directly assessed the use of (19)F NMR to achieve quantitative mass balance, metabolism and distribution information for fluorinated compounds without the need for radiolabeled synthesis/study. As a test case, the disposition of pefloxacin, a fluoroquinolone antibiotic, was evaluated in rats using quantitative (19)F NMR in parallel with a radiolabeled study. Urine, bile, and feces samples were collected over specific periods following oral administration of either 25 mg/kg [(14)C]pefloxacin or 25 mg/kg pefloxacin and were subsequently profiled by radioactivity or (19)F NMR, respectively...
February 10, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28177131/dispersive-liquid-liquid-microextraction-of-quinolones-in-porcine-blood-validation-of-a-ce-method-using-univariate-calibration-or-multivariate-curve-resolution-alternating-least-squares-for-overlapped-peaks
#15
Carla M Teglia, María S Cámara, Luciana Vera-Candioti
In the previously published part of this study, we detailed a novel strategy based on dispersive liquid-liquid microextraction to extract and preconcentrate nine fluoroquinolones in porcine blood. Moreover, we presented the optimized experimental conditions to obtain complete CE separation between target analytes. Consequently, this second part reports the validation of the developed method to determine flumenique, difloxacin, enrofloxacin, marbofloxacin, ofloxacin, ciprofloxacin, through univariate calibration, and enoxacin, danofloxacin, and gatifloxacin through multivariate curve resolution analysis...
February 8, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28176528/lc-ms-ms-determination-of-alectinib-and-its-major-human-metabolite-m4-in-human-urine-prevention-of-nonspecific-binding
#16
Katja Heinig, Denis Herzog, Luca Ferrari, Daniela Fraier, Kazuhiro Miya, Peter N Morcos
AIM: Alectinib (Alecensa(®)) is an anaplastic lymphoma kinase inhibitor for the treatment of anaplastic lymphoma kinase positive non-small-cell lung cancer, and M4 is its major pharmacologically active metabolite. To characterize the pharmacokinetics and excretion of alectinib and M4 in human urine, a bioanalytical method was required. RESULTS: An LC-MS/MS method using supported liquid extraction was developed for the determination of alectinib and M4 in human urine over the concentration range 0...
February 8, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28167558/pharmacokinetic-and-tissue-distribution-of-benznidazole-after-oral-administration-in-mice
#17
Luísa Perin, Rodrigo Moreira da Silva, Kátia da Silva Fonseca, Jamille Mirelle de Oliveira Cardoso, Fernando Augusto Siqueira Mathias, Levi Eduardo Soares Reis, Israel Molina, Rodrigo Correa-Oliveira, Paula Melo de Abreu Vieira, Cláudia Martins Carneiro
Specific chemotherapy using Benznidazole (BNZ) for Chagas disease during the chronic stage is controversial due to its limited efficacy and toxic effects. Although BNZ has been used to treat Chagas disease since the 1970s, few studies about the biodistribution of this drug exist. In this study, BNZ tissue biodistribution in a murine model and its pharmacokinetic profile in plasma were monitored. A bioanalytical high-performance liquid chromatography with an ultraviolet detector (HPLC-UV) method was developed and validated according to the European Medicines Agency for quantification of BNZ in organs and plasma samples prepared by liquid-liquid extraction using ethyl acetate...
February 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28167419/comparative-pharmacokinetic-profiles-of-selected-irreversible-tyrosine-kinase-inhibitors-neratinib-and-pelitinib-with-apigenin-in-rat-plasma-by-uplc-ms-ms
#18
Hadir M Maher, Nourah Z Alzoman, Shereen M Shehata, Ashwag O Abahussain
Neratinib (NER) and pelitinib (PEL) are irreversible tyrosine kinase inhibitors (TKIs) that have been recently employed in cancer treatment. Apigenin (API), among other flavonoids, is known to have antioxidant, anti-proliferative, and carcinogenic effect. API can potentiate the antitumor effect of chemotherapeutic agents and/or alleviate the side effects of many anticancer agents. Since TKIs are mostly metabolized by CYP3A4 enzymes and that API could alter the enzymatic activity, potential drug interactions could be expected following their co-aministration...
January 31, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28161751/miltefosine-a-novel-internal-standard-approach-to-lysophospholipid-quantitation-using-lc-ms-ms
#19
A Ruth Godfrey, Lewis Jones, Mairead Davies, Rachel Townsend
Understanding and determining levels of lysophospholipids (LPLs) is of increasing interest to the bioanalytical community as they may be targeted for preparative removal as a matrix interference or as a lead substance as a biomarker of disease. Studies monitoring levels of LPLs have used a range of approaches for quantitation whereby those using an internal standard have used either deuterated analogues of the target LPL or alternative LPLs containing an odd number of carbon atoms within its chain, which can be expensive and difficult to distinguish with other LPLs, respectively...
February 4, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28159531/a-validated-lc-ms-ms-method-for-the-estimation-of-glimepiride-and-pitavastatin-in-rat-plasma-application-to-drug-interaction-studies
#20
Shruti Surendran, David Paul, Ratna Sushmita, Lavanya Krishna, Nirbhay Kumar Tiwari, Sanjeev Giri, Nanjappan Satheeshkumar
Glimepiride (GLI) is prescribed for the management of type-2 diabetes where as pitavastatin (PIT) for the treatment of diabetes associated dyslipidemia. Both the drugs are metabolized by CYP2C9 and have the potential of altering the enzyme through either inhibition or induction. In this respect, we present a simple, fast and validated bioanalytical LC-MS/MS method for the simultaneous estimation of GLI and PIT from rat plasma. Waters XTerra RP HPLC column (4.6×100mm, 5μm) with mobile phase consisting of acetonitrile and 10mM ammonium acetate (pH-6...
January 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
keyword
keyword
88645
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"