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https://www.readbyqxmd.com/read/28812068/mechanistic-investigations-of-the-2-coumaranone-chemiluminescence
#1
Stefan Schramm, Isabelle Navizet, Durga Prasad Karothu, Pascal Oesau, Veronika Bensmann, Dieter Weiss, Rainer Beckert, Panče Naumov
2-Coumaranones are evolving as a new, efficient, versatile, and synthetically accessible platform for the next generation chemiluminescent probes. Despite the favorable quantum yields, the exact mechanism of their chemiluminescence remains elusive. Here, we analyze the details of the mechanism of the 2-coumaranone chemiluminescence using a combination of experimental and computational methods. By using EPR spectroscopy we show that superoxide radical anions are involved in the reactions, in support of the hypothesis that the mechanism includes a single electron transfer step...
August 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28809064/electrical-textile-valves-for-paper-microfluidics
#2
Alar Ainla, Mahiar M Hamedi, Firat Güder, George M Whitesides
This paper describes electrically-activated fluidic valves that operate based on electrowetting through textiles. The valves are fabricated from electrically conductive, insulated, hydrophobic textiles, but the concept can be extended to other porous materials. When the valve is closed, the liquid cannot pass through the hydrophobic textile. Upon application of a potential (in the range of 100-1000 V) between the textile and the liquid, the valve opens and the liquid penetrates the textile. These valves actuate in less than 1 s, require low energy (≈27 µJ per actuation), and work with a variety of aqueous solutions, including those with low surface tension and those containing bioanalytes...
August 15, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28795582/current-status-of-bioanalytical-outsourcing-a-pharma-perspective
#3
(no author information available yet)
Eric Woolf, Ajai Chaudhary and Sheila Breidinger from Merck, spoke to Sankeetha Nadarajah (Commissioning Editor, Bioanalysis) about outsourcing strategy implementation.
August 10, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28793756/pharmacokinetics-of-rhodamine-110-and-its-organ-distribution-in-rats
#4
Shiau-Han Jiang, Yung-Yi Cheng, Teh-Ia Huo, Tung-Hu Tsai
Rhodamine dyes have been banned as food additives due to their potential tumorigenicity. Rhodamine 110 is illegal as a food additive, although its pharmacokinetics have not been characterized, and no accurate bioanalytical methods are available to quantify rhodamine 110. The aim of this study was to develop and validate a fast, stable, sensitive method to quantify rhodamine 110 using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to assess its pharmacokinetics and organ distribution in awake rats...
August 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28792915/fast-rbc-loading-by-fluorescent-antibodies-and-nuclei-staining-dye-and-their-potential-bioanalytical-applications
#5
Mohamed K Al-Essa, Susanne Melzer, Attila Tarnok, Kamal A Hadidi, Mohammed El-Khateeb
This study was designed to load different antibodies (Abs) and a fluorescent dye onto the red blood cell (RBC) surface. We have used fluorescein isothiocyanate (FITC)-conjugate anti-human Ab, CD22-PE (B-cell marker-phycoerythrin Ab), and 4',6-diamidino-2-phenylindole (DAPI) for insertion over the RBC surface. In a first step, conjugation experiments were performed: in dimethyl sulfoxide (DMSO), RBCs were conserved and modified by succinic anhydride to create an additional -COOH group, and then activated with 3-(3-dimethylaminopropyl)carbodiimide-N-hydroxysuccinimide (EDC-NHS) in 2-(N-morpholino) ethanesulfonic acid hydrate buffer for insertion of labeled Abs or DAPI...
August 9, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28782940/electrokinetic-manipulation-of-silver-and-platinum-nanoparticles-and-their-stochastic-electrochemical-detection
#6
Jason Bonezzi, Tulashi Luitel, Aliaksei Boika
Electrokinetic phenomena such as dielectrophoresis and electrothermal fluid flow are used to increase the rate of mass transfer of silver and platinum nanoparticles and improve their stochastic electrochemical detection. These phenomena are induced by applying a high frequency alternating current (ac) waveform between a counter electrode and a working disk microelectrode. By recording chronoamperograms at room temperature and various ac powers, it is shown that the ac heating leads to an increase in the collision frequency of studied nanoparticles with working electrode surface by a factor of ∼10(1)-10(3) as well as the increase in the magnitude of the measured faradaic response...
August 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28781958/highly-sensitive-protein-detection-by-biospecific-afm-based-fishing-with-pulsed-electrical-stimulation
#7
Tatyana O Pleshakova, Kristina A Malsagova, Anna L Kaysheva, Arthur T Kopylov, Vadim Yu Tatur, Vadim S Ziborov, Sergey L Kanashenko, Rafael A Galiullin, Yuri D Ivanov
We report here the highly sensitive detection of protein in solution at concentrations from 10(-15) to 10(-18) m using the combination of atomic force microscopy (AFM) and mass spectrometry. Biospecific detection of biotinylated bovine serum albumin was carried out by fishing out the protein onto the surface of AFM chips with immobilized avidin, which determined the specificity of the analysis. Electrical stimulation was applied to enhance the fishing efficiency. A high sensitivity of detection was achieved by application of nanosecond electric pulses to highly oriented pyrolytic graphite placed under the AFM chip...
August 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28774619/total-organic-halogen-tox-in-human-urine-a-halogen-specific-method-for-human-exposure-studies
#8
Susana Y Kimura, Weiwei Zheng, Taylor N Hipp, Joshua M Allen, Susan D Richardson
Disinfection by-products (DBPs) are a complex mixture of compounds unintentionally formed as a result of disinfection processes used to treat drinking water. Effects of long-term exposure to DBPs are mostly unknown and were the subject of recent epidemiological studies. However, most bioanalytical methods focus on a select few DBPs. In this study, a new comprehensive bioanalytical method has been developed that can quantify mixtures of organic halogenated compounds, including DBPs, in human urine as total organic chlorine (TOCl), total organic bromine (TOBr), and total organic iodine (TOI)...
August 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28772226/liquid-chromatography-tandem-mass-spectrometric-assay-for-the-quantitative-determination-of-the-tyrosine-kinase-inhibitor-quizartinib-in-mouse-plasma-using-salting-out-liquid-liquid-extraction
#9
Irene A Retmana, Jing Wang, Alfred H Schinkel, Jan H M Schellens, Jos H Beijnen, Rolf W Sparidans
A bioanalytical assay for quizartinib -a potent, and selective FLT3 tyrosine kinase inhibitor- in mouse plasma was developed and validated. Salting-out assisted liquid-liquid extraction (SALLE), using acetonitrile and magnesium sulfate, was selected as sample pretreatment with deuterated quizartinib as internal standard. Separation was performed with reversed-phase liquid chromatography followed by detection with positive electrospray-triple quadrupole mass spectrometry in the selected reaction monitoring mode...
July 23, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28772158/novel-antioxidant-bromophenols-with-acetylcholinesterase-butyrylcholinesterase-and-carbonic-anhydrase-inhibitory-actions
#10
Necla Öztaskın, Parham Taslimi, Ahmet Maraş, İlhami Gülcin, Süleyman Göksu
In this study, a series of novel bromophenols were synthesized from benzoic acids and methoxylated bromophenols. The synthesized compounds were evaluated by using different bioanalytical antioxidant assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(+)) radical scavenging assays. Also, reducing power of novel bromophenols were evaluated by Cu(2+)-Cu(+) reducing, Fe(3+)-Fe(2+) reducing and [Fe(3+)-(TPTZ)2](3+)-[Fe(2+)-(TPTZ)2]2+ reducing and ferrous ions (Fe(2+)) chelating abilities...
July 20, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28766480/development-and-validation-of-an-hplc-uv-method-to-quantify-tavaborole-during-in-vitro-transungual-permeation-studies
#11
Silvia Tampucci, Eleonora Terreni, Susi Burgalassi, Patrizia Chetoni, Daniela Monti
A selective and rapid reversed-phase HPLC-UV method was developed and validated to quantify tavaborole (TAV; AN2690) in biological samples, i.e., in receiving phase and in bovine hoof membrane extract derived from in vitro transungual permeation studies. A simple solid-liquid extraction procedure was used to recover the drug from the bovine hoof slices. TAV chromatographic separation was achieved on a Luna PFP column (150 × 4.6 mm, 5 μm) using a mobile phase consisting of a 70% phosphoric acid solution (10 mM, pH 2...
August 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28766362/a-comparison-study-of-bioanalytical-methods-for-characterization-of-anti-idursulfase-antibodies
#12
Luying Pan, Kelly Hilton, Marie Nadeau, Thomas McCauley, Yongchang Qiu
AIM: Legacy methods with complex testing scheme for characterization of anti-idursulfase antibodies (ADA) were simplified and optimized in order to meet current regulatory guidance and provide more timely and cost-effective support for routine patient care. RESULTS: To compare the performance of the original and updated methods, patient samples receiving commercially prescribed Elaprase treatment were analyzed by both test methods. The ADA and neutralizing antibody results obtained by both methods were highly correlated and the updated method had an overall higher ADA and neutralizing antibody positive rates and higher ADA titers...
August 2, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28765879/mass-spectrometric-identification-of-dystrophin-the-protein-product-of-the-duchenne-muscular-dystrophy%C3%A2-gene-in-distinct-muscle-surface-membranes
#13
Sandra Murphy, Kay Ohlendieck
Supramolecular membrane complexes of low abundance are difficult to study by routine bioanalytical techniques. The plasmalemmal complex consisting of sarcoglycans, dystroglycans, dystrobrevins and syntrophins, which is closely associated with the membrane cytoskeletal protein dystrophin, represents such a high‑molecular‑mass protein assembly in skeletal muscles. The almost complete loss of the dystrophin isoform Dp427‑M and concomitant reduction in the dystrophin‑associated glycoprotein complex is the underlying cause of the highly progressive neuromuscular disorder named Duchenne muscular dystrophy...
July 27, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28763257/bioanalytical-outsourcing-transitioning-from-pharma-to-cro
#14
Roger Hayes
No abstract text is available yet for this article.
August 1, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28763250/bioanalytical-outsourcing-models-in-pharmaceutical-companies
#15
Dieter Zimmer
No abstract text is available yet for this article.
August 1, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28763245/bioanalytical-outsourcing-strategy-in-china
#16
Kelly Dong, Daniel Tang
No abstract text is available yet for this article.
August 1, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28763237/changing-shape-evolving-an-outsourced-bioanalytical-strategy-to-support-the-changing-needs-of-drug-development
#17
Craig Stovold, Fiona Milligan, Glen Hawthorne, Colin Pattison, Amanda Wilson
No abstract text is available yet for this article.
August 1, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28762758/outsourcing-bioanalytical-services-at-janssen-research-and-development-the-sequel-anno-2017
#18
Lieve Dillen, Tom Verhaeghe
The strategy of outsourcing bioanalytical services at Janssen has been evolving over the last years and an update will be given on the recent changes in our processes. In 2016, all internal GLP-related activities were phased out and this decision lead to the re-orientation of the in-house bioanalytical activities. As a consequence, in-depth experience with the validated bioanalytical assays for new drug candidates is currently gained together with the external partner, since development and validation of the assay and execution of GLP preclinical studies are now transferred to the CRO...
August 1, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28762755/astrazeneca-and-covance-laboratories-clinical-bioanalysis-alliance-an-evolutionary-outsourcing-model
#19
Cecilia Arfvidsson, Paul Severin, Victoria Holmes, Richard Mitchell, Christopher Bailey, Stephanie Cape, Yan Li, Tammy Harter
The AstraZeneca and Covance Laboratories Clinical Bioanalysis Alliance (CBioA) was launched in 2011 after a period of global economic recession. In this challenging environment, AstraZeneca elected to move to a full and centralized outsourcing model that could optimize the number of people supporting bioanalytical work and reduce the analytical cost. This paper describes the key aspects of CBioA, the innovative operational model implemented, and our ways of ensuring this was much more than simply a cost reduction exercise...
August 1, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28759057/recent-trends-in-electrochemiluminescence-aptasensors-and-their-applications
#20
Yasamin Nasiri Khonsari, Shiguo Sun
Aptamers are single stranded DNA or RNA ligands which can be selected for different targets from proteins to small organic dyes. In the past few years great progress has been accomplished in the development of aptamer based bioanalytical assays with different detection techniques. Among them, electrochemiluminescence (ECL) aptasensors are very promising because they have the advantages of both electrochemical and chemiluminescence biosensors, such as high sensitivity, low background, cost effectiveness, and ease of control...
July 31, 2017: Chemical Communications: Chem Comm
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