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https://www.readbyqxmd.com/read/27933362/coordination-induced-decomposition-of-luminescent-gold-nanoparticles-sensitive-detection-of-h2o2-and-glucose
#1
Xiaoqi Lai, Fengxian Luo, Yaping Wang, Xuxian Su, Jinbin Liu
The surface inert of luminescent gold nanoparticles (AuNPs) toward biomolecules set a challenge to further exploit their bioanalytical applications using the direct luminescence response. Herein, we report a novel approach to induce significant luminescence quenching of the AuNPs upon the interaction with a metal coordination ligand tris(2-carboxyethyl)phosphine (TCEP), providing a strategy for the detection of H2O2 with a limit of detection (LOD) of 14 nM through the reaction between H2O2 and TCEP to protect the luminescence quenching of the AuNPs...
December 8, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27925254/relative-tissue-distribution-and-excretion-studies-of-gastrodin-and-parishin-from-powder-and-extract-of-gastrodiae-rhizoma-in-rat-by-uhplc-esi-ms-ms
#2
Zhengmeng Jiang, Xiuyan Zheng, Xiaojian Gong, Chao Zhao, Xin Zhou, Yang Zhao, Yanfang Yan
New research indicated that Gastrodiae rhizome (GR) has potential anti-diabetic and anti-asthmatic effects in mice models. On the basis of our previously study of the relative bioavailability of gastrodin (GAS) and parishin (PA) from extract and powder of GR., we make further research on the tissue distribution and excretion studies of the two analytes. A reliable bioanalytical method for the quantification of GAS and PA in rat tissues and excretion is required. Chromatographic separation was carried out on a gradient mobile phase of acetonitrile-water with 0...
December 7, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27924550/detection-of-transcriptionally-active-mycotoxin-gene-clusters-dna-microarray
#3
Tamás Emri, Anna Zalka, István Pócsi
Various bioanalytical tools including DNA microarrays are frequently used to map global transcriptional changes in mycotoxin producer filamentous fungi. This effective hybridization-based transcriptomics technology helps researchers to identify genes of secondary metabolite gene clusters and record concomitant gene expression changes in these clusters initiated by versatile environmental conditions and/or gene deletions. Such transcriptional data are of great value when future mycotoxin control technologies are considered and elaborated...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27915473/urine-proteomics-in-the-era-of-mass-spectrometry
#4
REVIEW
Ashley Beasley-Green
With the technological advances of mass spectrometry (MS)-based platforms, clinical proteomics is one of the most rapidly growing areas in biomedical research. Urine proteomics has become a popular subdiscipline of clinical proteomics because it is an ideal source for the discovery of noninvasive disease biomarkers. The urine proteome offers a comprehensive view of the local and systemic physiology since the proteome is primarily composed of proteins/peptides from the kidneys and plasma. The emergence of MS-based proteomic platforms as prominent bioanalytical tools in clinical applications has enhanced the identification of protein-based urinary biomarkers...
November 2016: International Neurourology Journal
https://www.readbyqxmd.com/read/27907869/is-a-deuterated-internal-standard-appropriate-for-the-reliable-determination-of-olmesartan-in-human-plasma
#5
Edyta Piórkowska, Jacek Musijowski, Katarzyna Buś-Kwaśnik, Piotr J Rudzki
A right choice of the internal standard is one of the most challenging tasks during bioanalytical method development. Surprisingly, among the HPLC-MS methods for the determination of a cardiovascular drug olmesartan in plasma only structural analogues or similar compounds were used as internal standards. We have tried to answer the question whether the stable isotope labelled (deuterated) internal standard, as recommended by regulatory agencies, can be used for the reliable determination of olmesartan in human plasma...
November 16, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27907855/lc-ms-ms-assay-for-the-quantitation-of-the-tyrosine-kinase-inhibitor-neratinib-in-human-plasma
#6
Brian F Kiesel, Robert A Parise, Alvin Wong, Kiana Keyvanjah, Samuel Jacobs, Jan H Beumer
Neratinib is an orally available tyrosine kinase inhibitor targeting HER2 (ERBB2) and EGFR (ERBB). It is being clinically evaluated for the treatment of breast and other solid tumors types as a single agent or in combination with other chemotherapies. In support of several phase I/II clinical trials investigating neratinib combinations, we developed and validated a novel LC-MS/MS assay for the quantification of neratinib in 100μL of human plasma with a stable isotopic internal standard. Analytes were extracted from plasma using protein precipitation and evaporation of the resulting supernatant followed by resuspension...
November 22, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27898364/liquid-chromatography-tandem-mass-spectrometry-assay-for-the-quantification-of-niraparib-and-its-metabolite-m1-in-human-plasma-and-urine
#7
L van Andel, Z Zhang, S Lu, V Kansra, S Agarwal, L Hughes, M M Tibben, A Gebretensae, H Rosing, J H M Schellens, J H Beijnen
Niraparib (MK-4827) is a novel poly(ADP-Ribose) polymerase (PARP) inhibitor currently investigated in phase III clinical trials to treat cancers. The development of a new drug includes the characterisation of absorption, metabolism and excretion (AME) of the compound. AME studies are a requirement of regulatory agencies and for this purpose bioanalytical assays are essential. This article describes the development and validation of a bioanalytical assay for niraparib and its carboxylic acid metabolite M1 in human plasma and urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
November 19, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27888305/reversed-phase-separation-methods-for-glycan-analysis
#8
REVIEW
Gerda C M Vreeker, Manfred Wuhrer
Reversed-phase chromatography is a method that is often used for glycan separation. For this, glycans are often derivatized with a hydrophobic tag to achieve retention on hydrophobic stationary phases. The separation and elution order of glycans in reversed-phase chromatography is highly dependent on the hydrophobicity of the tag and the contribution of the glycan itself to the retention. The contribution of the different monosaccharides to the retention strongly depends on the position and linkage, and isomer separation may be achieved...
November 25, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27888146/a-rhodium-iii-based-inhibitor-of-autotaxin-with-antiproliferative-activity
#9
Tian-Shu Kang, Wanhe Wang, Hai-Jing Zhong, Jia-Xin Liang, Chung-Nga Ko, Jin-Jian Lu, Xiu-Ping Chen, Dik-Lung Ma, Chung-Hang Leung
BACKGROUND: Cancer of the skin is by far the most common of all cancers. Melanoma accounts for only about 1% of skin cancers but causes a large majority of skin cancer deaths. Autotaxin (ATX), also known as ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2), regulates physiological and pathological functions of lysophosphatidic acid (LPA), and is thus an important therapeutic target. METHODS: We synthesized ten metal-based complexes and a novel cyclometalated rhodium(III) complex 1 was identified as an ATX enzymatic inhibitor using multiple methods, including ATX enzymatic assay, thermal shift assay, western immunoblotting and so on...
November 23, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27885684/interference-free-mass-spectrometric-detection-of-capillary-isoelectric-focused-proteins-including-charge-variants-of-a-model-monoclonal-antibody
#10
Jens Hühner, Kevin Jooß, Neusüß Christian
CIEF represents an elegant technique especially for the separation of structural similar analytes, whereas MS is a state of the art instrumentation for the identification and characterization of biomolecules. The combination of both techniques can be realized by hyphenating CIEF with CZE-ESI-MS applying a mechanical valve. During the CZE step, the remaining ESI-interfering components of the CIEF electrolyte are separated from the analytes prior to MS detection. In this work, a multiple heart-cut approach is presented expanding our previous single heart-cut concept resulting in a dramatical reduction of analysis time...
November 25, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27884472/impact-of-untreated-wastewater-on-a-major-european-river-evaluated-with-a-combination-of-in%C3%A2-vitro-bioassays-and-chemical-analysis
#11
Maria König, Beate I Escher, Peta A Neale, Martin Krauss, Klára Hilscherová, Jiří Novák, Ivana Teodorović, Tobias Schulze, Sven Seidensticker, Muhammad Arslan Kamal Hashmi, Jörg Ahlheim, Werner Brack
Complex mixtures of micropollutants, including pesticides, pharmaceuticals and industrial chemicals emitted by wastewater effluents to European rivers may compromise the quality of these water resources and may pose a risk to ecosystem health and abstraction of drinking water. In the present study, an integrated analytical and bioanalytical approach was applied to investigate the impact of untreated wastewater effluents from the city of Novi Sad, Serbia, into the River Danube. The study was based on three on-site large volume solid phase extracted water samples collected upstream and downstream of the untreated wastewater discharge...
January 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27884077/from-patient-to-tube-the-importance-of-physiologically-relevant-quantitative-bioanalytical-assays
#12
Scott Summerfield, Matthew Barfield, Neil Spooner, Steve White
Circulating drug concentrations (clinical or preclinical) underly many interactions between industry and regulators; expressing safety coverage, pharmacokinetic-pharmacodynamic relationships or defining bioequivalence and dosing regimens. Accurate and precise measurement of these circulating concentrations is pivotal to the evolution and validation of any bioanalytical method that supports regulatory interactions. Since the bioanalyst is presented with a sub-aliquot of sampled biological matrix, how do they ensure this aliquot reflects the concentration in the subject at the time of collection? Here we share experiences from project support (internal and at CROs) that suggests we need to be ever vigilant translating the needs of bioanalysis with those of project teams...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27883257/determining-the-binding-affinities-of-prostate-specific-antigen-to-lectins-spr-and-microarray-approaches
#13
Pavel Damborský, Martina Zámorová, Jaroslav Katrlík
Prostate cancer (PCa) is one of the most common newly diagnosed cancer among men and we focused on its traditional biomarker, prostate-specific antigen (PSA), using targeted glycomics-based strategies. The aberrant glycosylation pattern of PSA may serve as valuable tool for improving PCa diagnosis including its early-stage. In the present work we evaluated the usability of two techniques, surface plasmon resonance and protein microarray assay, for the study and characterization of interactions of PSA (both free and complexed) with 6 lectins (SNA, ConA, RCA, AAL, WGA and MAA II)...
November 24, 2016: Proteomics
https://www.readbyqxmd.com/read/27882699/controlled-retention-and-release-of-biomolecular-transport-systems-using-shape-changing-polymer-bilayers
#14
Georgi Stoychev, Cordula Reuther, Stefan Diez, Leonid Ionov
Biomolecular transport systems based on cytoskeletal filaments and motor proteins have become promising tools for a wide range of nanotechnological applications. In this paper, we report control of such transport systems using substrates with switchable shape. We demonstrate this approach on the example of microtubules gliding on surfaces of self-folding polymer bilayers with adsorbed kinesin motors. The polymer bilayers are able to undergo reversible transitions between flat and tube-like shapes that allow the externally controlled retention and release of gliding microtubules...
November 24, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27868374/automated-coating-procedures-to-produce-poly-ethylene-glycol-brushes-in-fused-silica-capillaries
#15
Nicklas N Poulsen, Jesper Østergaard, Nickolaj J Petersen, Kim Daasbjerg, Joseph Iruthayaraj, Andra Dedinaite, Ricardas Makuska, Henrik Jensen
Many bioanalytical methods rely on electrophoretic separation of structurally labile and surface active biomolecules such as proteins and peptides. Often poor separation efficiency is due to surface adsorption processes leading to protein denaturation and surface fouling in the separation channel. Flexible and reliable approaches for preventing unwanted protein adsorption in separation science are thus in high demand. We therefore present new coating approaches based on an automated in-capillary surface initiated atom transfer radical polymerization process (covalent coating) as well as by electrostatically adsorbing a pre-synthesized polymer leading to functionalized molecular brushes...
November 21, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27862093/bioanalytical-method-for-the-estimation-of-co-administered-esomeprazole-leflunomide-and-ibuprofen-in-human-plasma-and-in-pharmaceutical-dosage-forms-using-micellar-liquid-chromatography
#16
Wael Talaat
The present study represents a connection between basic science and clinical applied science through providing a bioanalytical method for the analysis of certain co-administered drugs used for the treatment of rheumatoid arthritis. The studied drugs are esomeprazole, leflunomide and ibuprofen. The proposed bioanalytical method is a simple reversed phase high performance liquid chromatographic method using micellar mobile phase. The method is conducted using a Shim-pack VP-ODS (150 mm × 4.6 mm ID) stainless steel column at ambient temperature with ultraviolet detection at 285 nm...
October 13, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859537/development-of-a-targeted-method-for-quantification-of-gypenoside-xlix-in-rat-plasma-using-spe-and-lc-ms-ms
#17
Song Guo, Chengxu Sui, Ying Ma
A sensitive, selective and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of gypenoside XLIX, a naturally occurring gypenoside of Gynostemma pentaphyllum in rat plasma and then validated according to FDA Guidance for industry-bioanalytical method validation. Plasma samples were prepared by a simple solid-phase extraction. Separation was performed on a Waters XBridgeTM BEH C18 chromatography column (4.6 mm × 50 mm, 2.5 µm) using a mobile phase of acetonitrile and water (62...
November 16, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859448/nanotechnology-in-glycomics-applications-in-diagnostics-therapy-imaging-and-separation-processes
#18
REVIEW
Erika Dosekova, Jaroslav Filip, Tomas Bertok, Peter Both, Peter Kasak, Jan Tkac
This review comprehensively covers the most recent achievements (from 2013) in the successful integration of nanomaterials in the field of glycomics. The first part of the paper addresses the beneficial properties of nanomaterials for the construction of biosensors, bioanalytical devices, and protocols for the detection of various analytes, including viruses and whole cells, together with their key characteristics. The second part of the review focuses on the application of nanomaterials integrated with glycans for various biomedical applications, that is, vaccines against viral and bacterial infections and cancer cells, as therapeutic agents, for in vivo imaging and nuclear magnetic resonance imaging, and for selective drug delivery...
November 15, 2016: Medicinal Research Reviews
https://www.readbyqxmd.com/read/27859436/tissue-distribution-of-capilliposide-b-capilliposide-c-and-their-bioactive-metabolite-in-mice-using-liquid-chromatography-tandem-mass-spectrometry
#19
Zhongzhe Cheng, Xing Zhou, Bingying Hu, Wenyi Li, Guiying Chen, Yang Zhang, Jingkui Tian, Lin Zhang, Ming Li, Hongliang Jiang
Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activities in vivo and vitro. Capilliposide B (LC-B) and Capilliposide C (LC-C) were the main bioactive components in this plant. To explore their tissue distribution, a reliable bioanalytical method for the quantification of LC-B, LC-C, and their bioactive metabolite, Capilliposide A (LC-A) in mice tissues was developed and validated. In this study, the tissue distribution profiles of the three compounds were examined after intravenous administration of pure LC-B and oral administration of total saponins of L...
November 14, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27855972/transformation-products-in-the-water-cycle-and-the-unsolved-problem-of-their-proactive-assessment-a-combined-in-vitro-in-silico-approach
#20
Jakob Menz, Anju Priya Toolaram, Tushar Rastogi, Christoph Leder, Oliver Olsson, Klaus Kümmerer, Mandy Schneider
Transformation products (TPs) emerging from incomplete degradation of micropollutants in aquatic systems can retain the biological activity of the parent compound, or may even possess new unexpected toxic properties. The chemical identities of these substances remain largely unknown, and consequently, the risks caused by their presence in the water cycle cannot be assessed thoroughly. In this study, a combined approach for the proactive identification of hazardous elements in the chemical structures of TPs, comprising analytical, bioanalytical and computational methods, was assessed by the example of the pharmaceutically active micropollutant propranolol (PPL)...
November 15, 2016: Environment International
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