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https://www.readbyqxmd.com/read/29327589/transition-metal-dichalcogenide-nanosheets-for-visual-monitoring-pcr-rivaling-a-real-time-pcr-instrument
#1
Liu Wang, Zhicheng Huang, Rui Wang, Yibo Liu, Cheng Qian, Jian Wu, Juewen Liu
Monitoring the progress of polymerase chain reactions (PCR) is of critical importance in bioanalytical chemistry and molecular biology. While real-time PCR thermocyclers are ideal for this purpose, its high cost has limited applications in resource-poor areas. Direct visual detection would be a more attractive alternative. To monitor PCR amplification, DNA staining dyes such as SYBR Green I (SG) are often used. Although these dyes give higher fluorescence when binding to double-stranded (ds) DNA products, they also yield strong background fluorescence in the presence of a high concentration of single-stranded (ss) DNA primers...
January 12, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29322229/bioanalytical-verification-of-v-type-nerve-agent-exposure-simultaneous-detection-of-phosphonylated-tyrosines-and-cysteine-containing-disulfide-adducts-derived-from-human-albumin
#2
Andreas Kranawetvogl, Jim Küppers, Markus Siegert, Michael Gütschow, Franz Worek, Horst Thiermann, Paul W Elsinghorst, Harald John
Nerve agents still represent a serious threat to civilian and military personnel as demonstrated by the violent conflict in the Middle East. For verification of poisoning, covalent adducts with endogenous proteins (e.g., human serum albumin, HSA) are valuable long-term biomarkers. Accordingly, we developed a microbore liquid chromatography-electrospray ionization mass spectrometry/high-resolution mass spectrometry (μLC-ESI MS/HR MS) method for simultaneous detection of HSA-adducts with the V-type nerve agents VX, Chinese VX (CVX), and Russian VX (RVX)...
January 10, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29319234/comparative-pharmacokinetics-of-trandolapril-its-active-metabolite-and-verapamil-in-human-plasma-of-egyptian-population-using-hplc-ms-ms
#3
Ragaa Magdy, Ahmed H El-Khatib, Ahmed Hemdan, Omar Abd Elaziz, Maha Farouk, Michael W Linscheid
Trandolapril and verapamil are commonly used antihypertensive drugs. However, there is a lack of available data on the change of their pharmacokinetics in patients with liver or kidney impairment and hence the need for dose adjustment. In the presented work, a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the monitoring of trandolapril, its active metabolite trandolaprilat, and verapamil in human plasma of patients with renal impairment and/or liver insufficiency...
January 10, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29315954/development-and-validation-of-an-assay-to-analyze-atazanavir-in-human-hair-via-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms
#4
Nhi Phung, Karen Kuncze, Hideaki Okochi, Alexander Louie, Leslie Z Benet, Igho Ofokotun, David W Haas, Judith S Currier, Tariro D Chawana, Anandi N Sheth, Peter Bacchetti, Monica Gandhi, Howard Horng
Assays to quantify antiretrovirals in hair samples are increasingly used to monitor adherence and exposure in both HIV prevention and treatment studies. Atazanavir (ATV) is a protease inhibitor used in combination antiretroviral therapy (ART). We developed and validated a liquid chromatography tandem mass spectrometry (LC-MS/MS)-based method to quantify ATV in human hair, per the NIH Division of AIDS' Clinical Pharmacology Quality Assurance (CPQA) program and the FDA bioanalytical method validation guidelines...
January 9, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29315887/all-in-one-electroactive-nanocarbon-as-simultaneous-platform-and-label-for-single-step-biosensing
#5
Zhao Xuan Cheng, Alessandra Bonanni
We demonstrate here that an electroactive nanocarbon material can simultaneously work as both platform and label for the detection of mycotoxins. The versatility of the material for the immobilization of biorecognition elements was combined with its ability to provide an intrinsic electrochemical signal upon reduction of the oxygen functionalities on its surface. The intensity of peak current reflects the availability of oxygen functionalities for reduction, which can be directly correlated to the specific biorecognition event...
January 5, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29314090/development-and-validation-of-lc-ms-ms-method-for-simultaneous-determination-of-sofosbuvir-and-daclatasvir-in-human-plasma-application-to-pharmacokinetic-study
#6
Ola M Abdallah, Ahmed M Abdel-Megied, Amira S Gouda
The first simple and highly sensitive Liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalytical method was developed and fully validated for the simultaneous determination of newly discovered antiviral drugs namely, Sofosbuvir (SOF) and Daclatasvir (DAC) in human plasma. Tadalafil (TAD) was used as internal standard (IS). SOF, DAC and TAD (IS) were extracted from plasma using liquid-liquid extraction technique with methyl tert-butyl ether. The chromatographic separation was carried out using ZorbaxSB-C18 column (4...
January 3, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29313446/monoclonal-antibody-higher-order-structure-analysis-by-high-throughput-protein-conformational-array
#7
Yuanli Song, Deqiang Yu, Mukesh Mayani, Nesredin Mussa, Zheng Jian Li
The elucidation of antibody higher order structure (HOS) is critical in therapeutic antibody development. Since HOS determines the protein bioactivity and chemo-physical properties, this knowledge can help to ensure that the safety and efficacy attributes are not compromised. Protein conformational array (PCA) is a novel method for determining the HOS of monoclonal antibodies. Previously, we successfully utilized an enzyme-linked immunosorbent assay (ELISA)-based PCA along with other bioanalytical tools to elucidate the structures of antibody aggregates...
January 9, 2018: MAbs
https://www.readbyqxmd.com/read/29310217/uhplc-ms-and-maldi-ms-study-of-aluminum-phthalocyanine-chloride-and-development-of-a-bioanalytical-method-for-its-quantification-in-nanoemulsions-and-biological-matrices
#8
Karen R Py-Daniel, Javier Calvo, Carlos M Infante C, Osmindo R Pires Junior, Sergio E Moya, Ricardo B Azevedo
Metal phthalocyanines are promising components in photodynamic therapy. Aluminum phthalocyanine chloride (AlClPc) has been used to treat oral cancer in mice, human carious tissue, lung cancer cells and other conditions. To overcome the high hydrophobicity of AlClPc, phthalocyanine is often encapsulated in nanoformulations. Despite increased usage, little is known about the pharmacokinetics and biodistribution of AlClPc. The aim of this study was the development and validation of a UHPLC-MS method for the determination of AlClPc in solution after extraction from nanoformulations and biological matrices such as plasma and tissue...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29286476/using-extraordinary-optical-transmission-to-quantify-cardiac-biomarkers-in-human-serum
#9
Abhijeet Patra, Tao Ding, Minghui Hong, Arthur Mark Richards, Ten It Wong, Xiaodong Zhou, Chester Lee Drum
For a biosensing platform to have clinical relevance in point-of-care (POC) settings, assay sensitivity, reproducibility, and ability to reliably monitor analytes against the background of human serum are crucial. Nanoimprinting lithography (NIL) was used to fabricate, at a low cost, sensing areas as large as 1.5 mm x 1.5 mm. The sensing surface was made of high-fidelity arrays of nanoholes, each with an area of about 140 nm2. The great reproducibility of NIL made it possible to employ a one-chip, one-measurement strategy on 12 individually manufactured surfaces, with minimal chip-to-chip variation...
December 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29284083/ecotoxicology-goes-on-a-chip-embracing-miniaturized-bioanalysis-in-aquatic-risk-assessment
#10
Olivia Campana, Donald Wlodkowic
Biological and environmental sciences are, more than ever, becoming highly dependent on technological and multidisciplinary approaches that warrant advanced analytical capabilities. Microfluidic Lab-on-a-Chip technologies are perhaps one the most groundbreaking offshoots of bioengineering, enabling design of an entirely new generation of bioanalytical instrumentation. They represent a unique approach to combine micro-scale engineering and physics with specific biological questions, providing technological advances that allow for fundamentally new capabilities in the spatio-temporal analysis of molecules, cells, tissues and even small metazoan organisms...
December 28, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29283556/nanochannels-photoelectrochemical-biosensor
#11
Nan Zhang, Yi-Fan Ruan, Li-Bin Zhang, Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Nanochannels have brought new opportunities for biosensor development. Herein we present the novel concept of nanochannels photoelectrochemical (PEC) biosensor based on the integration of a unique CuxO nanopyramid islands (NPIs) photocathode, the anodic aluminum oxide (AAO) membrance, and the alkaline phosphatase (ALP) catalytic chemistry. The CuxO NPIs photocathode possessed good performance, and further assembly with AAO will yield the designed architecture composed of vertically-aligned, highly-ordered nanoarrays bottomed with CuxO NPIs film...
December 28, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29261171/fluorescence-sensing-using-dna-aptamers-in-cancer-research-and-clinical-diagnostics
#12
REVIEW
Domenica Musumeci, Chiara Platella, Claudia Riccardi, Federica Moccia, Daniela Montesarchio
Among the various advantages of aptamers over antibodies, remarkable is their ability to tolerate a large number of chemical modifications within their backbone or at the termini without losing significant activity. Indeed, aptamers can be easily equipped with a wide variety of reporter groups or coupled to different carriers, nanoparticles, or other biomolecules, thus producing valuable molecular recognition tools effective for diagnostic and therapeutic purposes. This review reports an updated overview on fluorescent DNA aptamers, designed to recognize significant cancer biomarkers both in soluble or membrane-bound form...
December 20, 2017: Cancers
https://www.readbyqxmd.com/read/29258686/bioprofiling-of-salvia-miltiorrhiza-via-planar-chromatography-linked-to-bio-assays-high-resolution-mass-spectrometry-and-nuclear-magnetic-resonance-spectroscopy
#13
Ebrahim Azadniya, Gertrud E Morlock
An affordable bioanalytical workflow supports the collection of data on active ingredients, required for the understanding of health-related food, superfood and traditional medicines. Targeted effect-directed responses of single compounds in a complex sample highlight this powerful bioanalytical hyphenation of planar chromatography with (bio)assays. Among many reports about biological properties of Salvia miltiorrhiza Bunge root (Danshen) and their analytical methods, the highly efficient direct bioautography (DB) workflow has not been considered so far...
December 6, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29258285/point-of-care-diagnostics-recent-advances-and-trends
#14
EDITORIAL
Sandeep Kumar Vashist
Recent years have witnessed tremendous advances in point-of-care diagnostics (POCD), which are a result of continuous developments in biosensors, microfluidic, bioanalytical platforms, assay formats, lab-on-a-chip technologies, and complementary technologies. This special issue targets the critical advances in POCD and provides guided insights and directions for future research.
December 18, 2017: Biosensors
https://www.readbyqxmd.com/read/29248770/development-and-validation-of-an-uhplc-esi-qtof-ms-method-for-quantification-of-the-highly-hydrophilic-amyloid-%C3%AE-oligomer-eliminating-all-d-enantiomeric-peptide-rd2-in-mouse-plasma
#15
Michelle Hupert, Anne Elfgen, Elena Schartmann, Sarah Schemmert, Brigitte Buscher, Janine Kutzsche, Dieter Willbold, Beatrix Santiago-Schübel
During preclinical drug development, a method for quantification of unlabeled compounds in blood plasma samples from treatment or pharmacokinetic studies in mice is required. In the current work, a rapid, specific, sensitive and validated liquid chromatography mass-spectrometric UHPLC-ESI-QTOF-MS method was developed for the quantification of the therapeutic compound RD2 in mouse plasma. RD2 is an all-D-enantiomeric peptide developed for the treatment of Alzheimer's disease, a progressive neurodegenerative disease finally leading to dementia...
December 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29248769/an-improved-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms-method-for-quantification-of-dexmedetomidine-concentrations-in-samples-of-human-plasma
#16
Seyed Mojtaba Moosavi, Kiran Shekar, John F Fraser, Maree T Smith, Sussan Ghassabian
Dexmedetomidine (DMET) is a sedative, analgesic and anxiolytic with minimum adverse respiratory effects. An LC-MS/MS bioanalytical method has been developed and validated to accurately measure DMET concentrations in samples of human plasma. The method overcomes difficulties in the extraction and quantification of DMET due to the fact that it binds strongly to glass and plastic tubes, as well as solid phase extraction (SPE) cartridges. Human plasma (50 μL) was mixed with the internal standard (IS) (DMET-d4) solution (100 μL) and 0...
December 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29241088/development-of-an-lc-ms-method-to-quantify-coproporphyrin-i-and-iii-as-endogenous-biomarkers-for-drug-transporter-mediated-drug-drug-interactions
#17
Emmanuel Njumbe Ediage, Lieve Dillen, Ann Vroman, Luc Diels, Annett Kunze, Jan Snoeys, Tom Verhaeghe
Coproporphyrins are proposed as endogenous biomarkers of hepatic Organic Anion Transporting Polypeptide (OATP)1B functional activity. In this study, a new sample extraction method based on a mixed-mode anion exchange sorbent (SPE clean-up using Oasis 30mg Max 96 well plates) was developed for absolute quantification of coproporphyrin I and III (CP-I and CP-III) in human plasma. Chromatographic separation was performed with an Ace Excel 2 C18 PFP, 3μm, 2.1×150mm, maintained at 60°C. A 10mM ammonium formate containing 0...
December 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29239666/highly-selective-and-sensitive-measurement-of-active-forms-of-fgf21-using-novel-immunocapture-enrichment-with-lc-ms-ms
#18
Yue Zhao, Guowen Liu, Suk Kwok, Barry R Jones, Jane Liu, David Marchisin, Philip E Joyce, Jon Peterson, Jim X Shen
AIM: Recombinant FGF21 analogs are under wide ranging investigations as a potential therapeutic agent for Type 2 diabetes, as well as other metabolic disorders. The endogenous FGF21 is often used as a surrogate pharmacodynamic(PD) biomarker to assess drug efficacy and safety. Results & methodology: Immunocapture was performed using a monoclonal antibody which had been generated to bind to specific domain of native FGF21 as the capture reagent. After immunocapture, enzymatic digestion was performed and a native FGF21-specific tryptic peptide was monitored using LC-MS/MS by selective reaction monitoring...
January 2018: Bioanalysis
https://www.readbyqxmd.com/read/29236519/supercritical-fluid-chromatography-a-promising-alternative-to-current-bioanalytical-techniques
#19
Amandine Dispas, Hugues Jambo, Sébastien André, Eva Tyteca, Philippe Hubert
During the last years, chemistry was involved in the worldwide effort toward environmental problems leading to the birth of green chemistry. In this context, green analytical tools were developed as modern Supercritical Fluid Chromatography in the field of separative techniques. This chromatographic technique knew resurgence a few years ago, thanks to its high efficiency, fastness and robustness of new generation equipment. These advantages and its easy hyphenation to MS fulfill the requirements of bioanalysis regarding separation capacity and high throughput...
December 13, 2017: Bioanalysis
https://www.readbyqxmd.com/read/29231136/stability-of-drugs-of-abuse-in-urine-samples-at-room-temperature-by-use-of-a-salt-mixture
#20
Manuela Pellegrini, Silvia Graziano, Luisa Mastrobattista, Adele Minutillo, Francesco Paolo Busardo, Gianfranco Scarsella
BACKGROUND: It has been long recognized that ensuring analyte stability is of crucial importance in the use of any quantitative bioanalytical method. As analyses are usually not performed directly after collection of the biological samples, but after these have been processed and stored, it is essential that analyte stability be maintained over the relevant storage conditions in order to ensure that the obtained concentration results adequately reflect those directly after sampling. The conservation of urine samples in refrigerated/frozen conditions is strongly recommended; but not always feasible...
December 11, 2017: Current Pharmaceutical Biotechnology
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