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https://www.readbyqxmd.com/read/28344828/lc-ms-ms-assay-for-quantitation-of-enalapril-and-enalaprilat-in-plasma-for-bioequivalence-study-in-indian-subjects
#1
Dhiman Halder, Shubhasis Dan, Murari Mohun Pal, Easha Biswas, Nilendra Chatterjee, Pradipta Sarkar, Umesh Chandra Halder, Tapan Kumar Pal
BACKGROUND: Enalapril (EPL) is an angiotensin-converting enzyme inhibitor for the treatment of hypertension and chronic heart failure. Enalaprilat (EPLT) is an active metabolite that contributes to the overall activity of EPL. AIM: To quantitate EPL along with its metabolite EPLT using LC-MS/MS, a bioanalytical method was developed and validated with tolbutamide in human plasma using a protein precipitation technique. RESULTS: The sensitive and selective method has an LLOQ of 1 ng/ml with a linearity range of 1-500 ng/ml for both EPL and EPLT using 300 µl of plasma without any matrix effect...
March 2017: Future Science OA
https://www.readbyqxmd.com/read/28344632/dynamic-and-temporal-assessment-of-human-dried-blood-spot-ms-ms-all-shotgun-lipidomics-analysis
#2
Fei Gao, Justice McDaniel, Emily Y Chen, Hannah E Rockwell, Jeremy Drolet, Vivek K Vishnudas, Vladimir Tolstikov, Rangaprasad Sarangarajan, Niven R Narain, Michael A Kiebish
BACKGROUND: Real-time and dynamic assessment of an individual's lipid homeostatic state in blood is complicated due to the need to collect samples in a clinical environment. In the context of precision medicine and population health, tools that facilitate sample collection and empower the individual to participate in the process are necessary to complement advanced bioanalytical analysis. The dried blood spot (DBS) methodology via finger prick or heel prick is a minimally invasive sample collection method that allows the relative ease and low cost of sample collection as well as transport...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28344361/the-interaction-of-fluorescent-nanodiamond-probes-with-cellular-media
#3
Simon R Hemelaar, Andreas Nagl, François Bigot, Melissa M Rodríguez-García, Marcel P de Vries, Mayeul Chipaux, Romana Schirhagl
Fluorescent nanodiamonds (FNDs) are promising tools to image cells, bioanalytes and physical quantities such as temperature, pressure, and electric or magnetic fields with nanometer resolution. To exploit their potential for intracellular applications, the FNDs have to be brought into contact with cell culture media. The interactions between the medium and the diamonds crucially influence sensitivity as well as the ability to enter cells. The authors demonstrate that certain proteins and salts spontaneously adhere to the FNDs and may cause aggregation...
2017: Mikrochimica Acta
https://www.readbyqxmd.com/read/28343420/portable-devices-and-mobile-instruments-for-infectious-diseases-point-of-care-testing
#4
Luc Bissonnette, Michel G Bergeron
Rapidity, simplicity, and portability are highly desirable characteristics of tests and devices designed for performing diagnostics at the point of care (POC), either near patients managed in healthcare facilities or to offer bioanalytical alternatives in external settings. By reducing the turnaround time of the diagnostic cycle, POC diagnostics can reduce the dissemination, morbidity, and mortality of infectious diseases and provide tools to control the global threat of antimicrobial resistance. Areas covered: A literature search of PubMed and Google Scholar, and extensive mining of specialized publications, Internet resources, and manufacturers' websites have been used to organize and write this overview of the challenges and requirements associated with the development of portable sample-to-answer diagnostics, and showcase relevant examples of handheld devices, portable instruments, and less mobile systems which may or could be operated at POC...
March 27, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28343347/bioanalysis-of-farnesyl-pyrophosphate-in-human-plasma-by-high-performance-liquid-chromatography-coupled-to-triple-quadrupole-tandem-mass-spectrometry-and-hybrid-quadrupole-orbitrap-high-resolution-mass-spectrometry
#5
Hiroshi Sugimoto, Mie Iguchi, Fumihiro Jinno
The isoprenoids farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP) are pivotal intermediates for cholesterol homeostasis and cell signaling in the mevalonate pathway. We developed a sensitive and selective high-performance liquid chromatography tandem triple quadrupole mass spectrometry (LC-QQQ-MS) method for FPP in human plasma without the need for a derivatization process. We optimized the sample preparation procedure to extract FPP and (13)C5-FPP (as internal standard) from sample fluids using methanol...
March 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28341989/a-fully-validated-bioanalytical-method-using-an-uhplc-ms-ms-system-for-quantification-of-dna-and-rna-oxidative-stress-biomarkers
#6
Barbora Cervinkova, Lenka Kujovska Krcmova, Veronika Sestakova, Dagmar Solichova, Petr Solich
A new, rapid and effective ultra-high-performance liquid chromatography method with mass spectrometry detection is described for the separation and quantification of 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine and creatinine in human urine. The present study uses an isotope-labelled internal standard ([(15)N]5-8-hydroxy-2-deoxyguanosine), a BIO core-shell stationary phase and an isocratic elution of methanol and water. Sample preparation of human urine was performed by solid-phase extraction (SPE) on Oasis HLB cartridges with methanol/water 50:50 (v/v) elution...
March 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28341309/polymorphic-distribution-of-proteins-in-solution-by-mass-spectrometry-the-analysis-of-insulin-analogues
#7
Maely P Fávero-Retto, Luiz Henrique Guerreiro, Cássio M Pessanha, Leonardo C Palmieri, Luís Maurício T R Lima
The characterization of conformational and oligomeric distribution of proteins is of paramount importance for the understanding of the correlation between structure and function. Among the bioanalytical approaches currently available, the electrospray ionization-mass spectrometry (ESI-MS) coupled to ion mobility spectrometry (IMS) is the best suited for high resolution identification with high sensitivity, allowing the in situ separation of oligomeric and conformational species. We tested the performance of the ESI-MS technique along with the IMS separation approach on a broad variety of insulin and insulin analogues with distinct oligomeric distribution pattern...
January 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28340309/analytical-techniques-in-lipidomics-state-of-the-art
#8
Kamil Jurowski, Kamila Kochan, Justyna Walczak, Małgorzata Barańska, Wojciech Piekoszewski, Bogusław Buszewski
Current studies related to lipid identification and determination, or lipidomics in biological samples, are one of the most important issues in modern bioanalytical chemistry. There are many articles dedicated to specific analytical strategies used in lipidomics in various kinds of biological samples. However, in such literature, there is lack of articles dedicated to a comprehensive review of the actual analytical methodologies used in lipidomics. The aim of this article is to characterize the lipidomics methods used in modern bioanalysis according to the methodological point of view: 1) chromatography/separation methods, 2) spectroscopic methods and 3) mass spectrometry and also hyphenated methods...
March 24, 2017: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/28339299/the-10th-gcc-closed-forum-rejected-data-gcp-in-bioanalysis-extract-stability-bav-processed-batch-acceptance-matrix-stability-critical-reagents-eln-and-data-integrity-and-counteracting-fraud
#9
Stephanie Cape, Rafiq Islam, Corey Nehls, John Allinson, Afshin Safavi, Patrick Bennett, James Hulse, Chris Beaver, Masood Khan, Shane Karnik, Maria Cruz Caturla, Steve Lowes, Adriana Iordachescu, Luigi Silvestro, Rabab Tayyem, Ron Shoup, Stephanie Mowery, Anahita Keyhani, Andrea Wakefield, Yinghe Li, Jennifer Zimmer, Javier Torres, Philippe Couerbe, Ardeshir Khadang, James Bourdage, Nicola Hughes, Kayode Awaiye, Brent Matthews, Saadya Fatmi, Rhonda Johnson, Christina Satterwhite, Mathilde Yu, Jenny Lin, Laura Cojocaru, Michele Fiscella, Eric Thomas, Kai Kurylak, John Kamerud, Zhongping John Lin, Wei Garofolo, Natasha Savoie, Mike Buonarati, Nadine Boudreau, Clark Williard, Yansheng Liu, Dominic Warrino, Prashant Kale, Neil Adcock, Radha Shekar, Edward O'Connor, Hanna Ritzen, Christina Sanchez, Roger Hayes, Mohammed Bouhajib, Simona Rizea Savu, Bruce Stouffer, Edward Tabler, Jing Tu, Chad Briscoe, Barry van der Strate, Paul Rhyne, Phyllis Conliffe, Ira DuBey, Jim Yamashita, Daniel Tang, Elizabeth Groeber, Jenifer Vija, Michele Malone, Mohamed Osman
The 10th Global CRO Council (GCC) Closed Forum was held in Orlando, FL, USA on 18 April 2016. In attendance were decision makers from international CRO member companies offering bioanalytical services. The objective of this meeting was for GCC members to meet and discuss scientific and regulatory issues specific to bioanalysis. The issues discussed at this closed forum included reporting data from failed method validation runs, GCP for clinical sample bioanalysis, extracted sample stability, biomarker assay validation, processed batch acceptance criteria, electronic laboratory notebooks and data integrity, Health Canada's Notice regarding replicates in matrix stability evaluations, critical reagents and regulatory approaches to counteract fraud...
March 24, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28332837/molecular-recognition-based-dna-nanoassemblies-on-the-surfaces-of-nanosized-exosomes
#10
Shuo Wan, Liqin Zhang, Sai Wang, Yuan Liu, Cuichen Wu, Cheng Cui, Hao Sun, Muling Shi, Ying Jiang, Long Li, Liping Qiu, Weihong Tan
Exosomes are membrane-enclosed extracellular vesicles derived from cells, carrying biomolecules that include pro-teins and nucleic acids for intercellular communication. Owning to their advantages of size, structure, stability and biocompatibility, exosomes have been used widely as natu-ral nanocarriers for intracellular delivery of theranostic agents. Meanwhile, surface modifications needed to endow exosomes with additional functionalities remain challeng-ing by their small size and the complexity of their mem-brane surfaces...
March 23, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28322497/single-molecule-force-spectroscopy-to-compare-natural-versus-artificial-antibody-antigen-interaction
#11
Congzhou Wang, Rong Hu, Jeremiah J Morrissey, Evan D Kharasch, Srikanth Singamaneni
Biorecognition is central to various biological processes and finds numerous applications in virtually all areas of chemistry, biology, and medicine. Artificial antibodies, produced by imprinting synthetic polymers, are designed to mimic the biological recognition capability of natural antibodies, while exhibiting superior thermal, chemical, and environmental stability compared to their natural counterparts. The binding affinity of the artificial antibodies to their antigens characterizes the biorecognition ability of these synthetic nanoconstructs and their ability to replace natural recognition elements...
March 21, 2017: Small
https://www.readbyqxmd.com/read/28316235/evaluation-of-nanobody-conjugates-and-protein-fusions-as-bioanalytical-reagents
#12
Virginia J Bruce, Brian R McNaughton
Enzyme-linked immunosorbent assay (ELISA), flow cytometry, and Western blot are common bioanalytical techniques. Successful execution traditionally requires the use of one or more commercially available antibody-small-molecule dye, or antibody-reporter protein conjugates that recognize relatively short peptide tags (<15 amino acids). However, the size of antibodies, and their molecular complexity (by virtue of post-translational disulfide formation and glycosylation) typically requires either expression in mammalian cells or purification from immunized mammals...
March 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28315316/label-free-and-rapid-detection-of-atp-based-on-structure-switching-of-aptamers
#13
Danyang Ji, Hongqi Wang, Jia Ge, Lin Zhang, Jianjun Li, Dongmei Bai, Juan Chen, Zhaohui Li
In this work, an aptamer-based fluorescent strategy for label-free detection of ATP was developed by using Thioflavin T (ThT) as a fluorescence indicator, which can specifically bind with G-quadruplex DNAs to generate enhanced fluorescence intensity. In the absence of ATP, the folded structure of ATP aptamer allows the intercalation of ThT to produce strong fluorescence signal. However, upon ATP binding to the aptamer where ThT intercalated, the conformational change or distortion of the aptamer is large enough to cause much less intercalation of ThT and consequently drastic suppression of the fluorescence intensity...
March 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28301754/bioanalytical-measurements-enabled-by-surface-enhanced-raman-scattering-sers-probes
#14
Lauren E Jamieson, Steven M Asiala, Kirsten Gracie, Karen Faulds, Duncan Graham
Since its discovery in 1974, surface-enhanced Raman scattering (SERS) has gained momentum as an important tool in analytical chemistry. SERS is used widely for analysis of biological samples, ranging from in vitro cell culture models, ex vivo tissue and blood samples, and direct in vivo application. New insights have been gained into biochemistry, with an emphasis on biomolecule detection, from small molecules such as glucose and amino acids to larger biomolecules such as DNA, proteins, and lipids. These measurements have increased our understanding of biological systems, and significantly, they have improved diagnostic capabilities...
February 23, 2017: Annual Review of Analytical Chemistry
https://www.readbyqxmd.com/read/28300420/a-new-lc-ms-ms-bioanalytical-method-for-atenolol-in-human-plasma-and-milk
#15
Ei Mon Phyo Lwin, Cobus Gerber, Yunmei Song, Catherine Leggett, Usha Ritchie, Sean Turner, Sanjay Garg
AIM: A new sensitive LC-MS/MS method for the quantification of atenolol in human plasma and milk has been developed for clinical lactation studies. METHODS & RESULTS: Atenolol and the internal standard, phenazone, were extracted from biological matrices by protein precipitation. A Phenomenex(®) C-18 column and gradient chromatographic conditions were used for separation of the analyte, followed by detection with MS. Stability of samples was confirmed for atenolol in human plasma and milk for up to 3 months...
March 16, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28300418/incurred-sample-reanalysis-of-fingolimod-and-fingolimod-phosphate-in-blood-stability-evaluation-and-application-to-a-rat-pharmacokinetic-study
#16
Ramakrishna Nirogi, Nagasurya Prakash Padala, Devender Reddy Ajjala, Rajesh Kumar Boggavarapu, Praveen Kunduru
BACKGROUND: Incurred sample reanalysis (ISR) is an in-study validation parameter, which reinforces that the validated bioanalytical methods are reproducible. ISR of whole blood samples is complex when the test compounds can interconvert, ex vivo. Fingolimod and fingolimod phosphate are highly distributed in the blood cellular components and undergo rapid interconversion, both in vivo and ex vivo. An LC-MS/MS method capable of simultaneous quantification of fingolimod and fingolimod phosphate with the controlled sample preparation procedure is essential...
March 16, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28295098/optofluidic-biomolecule-sensors-based-on-a-si-h-microrings-embedded-in-silicon-glass-microchannels
#17
T Lipka, L Moldenhauer, L Wahn, H K Trieu
The large-scale and low-cost fabrication of high sensitivity sensors for the real-time detection of biochemicals and molecular substances opens up new opportunities in the areas of bioanalytic screening and medical diagnostics. Planar integrated photonic resonators that can be fabricated with a low footprint, in spatial and wavelength multiplexed arrangements, and that enable integration with microfluidics on the wafer scale have emerged as a promising sensing platform for these application fields. We realized an optofluidic and label-free biosensor that is based on hydrogenated amorphous silicon microring resonators embedded in silicon/glass microfluidic channels for analyte injection and biomolecule immobilization...
March 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28294502/the-role-of-anion-adsorption-in-the-effect-of-electrode-potential-on-spr-response
#18
Veronika K Laurinavichyute, Shavkat Nizamov, Vladimir M Mirsky
Surface plasmon resonance, being widely used in bioanalytics and biotechnology, is influenced by electrical potential of the resonant gold layer. To evaluate the mechanism of this effect, we have studied it in the solution of various inorganic electrolytes. The magnitude of the effect was decreased according to the series: KBr > KCl > KF > NaClO4. The data were treated using different models of the interface. Quantitative description was obtained for the model which takes into account local dielectric function of gold affected by free electron charge, diffuse ionic layer near gold / water interface and specific adsorption of halides to the gold surface with partial charge transfer...
March 11, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28291630/ceftriaxone-pharmacokinetics-by-new-simple-and-sensitive-ultra-high-performance-liquid-chromatography-method
#19
Michel Leandro Campos, Juliana de Moura Alonso, Evelin Dos Santos Martins, Jonata Augusto Oliveira, Carlos Alberto Hussni, Rosângela Gonçalves Peccinini
Ceftriaxone is a cephalosporin antibiotic with a potent antimicrobial activity and excellent penetration in most body fluids such as pleural, peritoneal, spinal and brain. These facts contribute to the application of ceftriaxone in the treatment of bacterial peritonitis, an abdominal disorder in veterinary medicine, with potential risk of death. The determination of ceftriaxone levels in plasma and peritoneal fluid may be used to assess the pharmacokinetic profile at various instances of administration and allows observing if the concentrations needed are being achieved...
February 27, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28281873/resolving-the-micro-heterogeneity-and-structural-integrity-of-monoclonal-antibodies-by-hybrid-mass-spectrometric-approaches
#20
Yang Yang, Guanbo Wang, Ting Song, Carlito B Lebrilla, Albert J R Heck
For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control. Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb. As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels...
February 14, 2017: MAbs
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