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prp fat graft

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https://www.readbyqxmd.com/read/27833274/effect-of-external-volume-expansion-on-the-survival-of-fat-grafts
#1
Raghuveer Reddy, Subramania Iyer, Mohit Sharma, Sundeep Vijayaraghavan, P Kishore, Jimmy Mathew, A K K Unni, P Reshmi, Rakesh Sharma, Chaya Prasad
INTRODUCTION: External volume expansion (EVE) is one method, which has been utilised for increasing the survival of adipose tissue grafts. EVE releases positive pressure from the graft and also induces intense levels of edema that decreases diffusion of metabolites essential for graft survival initially. The ideal timing of external volume expansion in relation to the injection of the fat to facilitate survival is not yet clear. AIMS AND OBJECTIVES: This study was undertaken to evaluate and compare the efficacy of external volume expansion applied at variable time points in relation to the injection of the fat...
May 2016: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
https://www.readbyqxmd.com/read/27828600/is-the-combination-of-fat-grafts-and-platelet-rich-plasma-effective-in-rats
#2
Alexandre Roriz Blumenschein, Ruffo Freitas-Junior, Marise Amaral Rebouças Moreira, Maria-Auxiliadora Paula Carneiro Cysneiros, Roseana Netto Pereira, Andrea Thomazine Tufanin, Leonardo Ribeiro Soares
PURPOSE: To investigate if the association of fat grafts and platelet-rich plasma (PRP) improves graft viability in female rats. METHODS: This is an experimental, randomized and blinded study, which involved 47 rats. Fat was harvested from the inguinal region and grafted to the cranial region. The experimental group consisted of PRP-enriched fat grafts (n=22) whilst the control group consisted of fat graft only (n=25). After a 100-day period, the animals were euthanised and the fat grafts were analyzed using scores from 0 (absent) to 4 (abundant), in optical microscopy by two independent and blinded pathologists...
October 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27363761/fat-stem-cells-and-platelet-rich-plasma
#3
REVIEW
Isaac B James, Sydney R Coleman, J Peter Rubin
The ideal filler for aesthetic surgery is inexpensive and easy to obtain, natural in appearance and texture, immunologically compatible, and long lasting without risk of infection. By most metrics, autologous fat grafts meet these criteria perfectly. Although facial fat grafting is now a commonly accepted surgical procedure, there has been a wave of activity applying stem cells and platelet-rich plasma (PRP) therapies to aesthetic practice. This article addresses technical considerations in the use of autologous fat transfer for facial rejuvenation, and also explores the current evidence for these stem cell and PRP therapies in aesthetic practice...
July 2016: Clinics in Plastic Surgery
https://www.readbyqxmd.com/read/26466811/applications-of-platelet-rich-plasma-in-dermatology-a-critical-appraisal-of-the-literature
#4
REVIEW
M D Lynch, S Bashir
Platelet-rich plasma (PRP) is an autologous blood-derived product enriched in platelets, growth factors, chemokines and cytokines. Initial applications were predominantly in musculoskeletal and maxillofacial fields, however in recent years, it has been used for a range of dermatological indications including wound healing, fat grafting, alopecia, scar revision and dermal volume augmentation. Here, we critically appraise the literature relating to the usage of PRP within Dermatology. We have evaluated in vitro data, preclinical animal studies and human trials...
2016: Journal of Dermatological Treatment
https://www.readbyqxmd.com/read/26416350/the-effects-of-platelet-rich-plasma-on-cell-proliferation-and-adipogenic-potential-of-adipose-derived-stem-cells
#5
Han Tsung Liao, Isaac B James, Kacey G Marra, J Peter Rubin
BACKGROUND: Platelet-rich plasma (PRP) contains multiple growth factors and has been shown to enhance fat graft survival after lipotransfer. However, the molecular mechanisms mediating this effect remain unknown. Adipose-derived stem cells (ASCs) play an important role in fat graft survival and are a likely target for PRP-mediated effects. This study seeks to investigate the impact of PRP on ASC proliferation and adipogenic differentiation. METHODS: Human ASCs were isolated using our laboratory protocol...
November 2015: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/26335660/the-safety-and-efficacy-of-cell-assisted-fat-grafting-to-traditional-fat-grafting-in-the-anterior-mid-face-an-indirect-assessment-by-3d-imaging
#6
Gordon H Sasaki
BACKGROUND: Numerous methodologies and algorithms have been suggested to enhance fat graft survival, including the usage of stromal vascular fraction (SVF) and platelet-rich plasma (PRP), but no long-term studies are available. OBJECTIVES: This single-center prospective, case-controlled study investigated the safety and efficacy of combining a modified Baker-designed lateral SMASectomy or plication face lift with simultaneous anterior mid-face grafting into site-specific compartments by (1) conventional Coleman's technique or (2) Yoshimura's cell-assisted lipografting technique...
December 2015: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/26335318/new-and-successful-technique-for-the-management-of-parry-romberg-syndrome-s-soft-tissue-atrophy
#7
Viviana Gómez Ortega, Daniel Sastoque
UNLABELLED: Parry-Romberg syndrome (PRS) is an infrequent, acquired disorder characterized by progressive hemiatrophy of the skin and soft tissue of the face and, in some patients, results in atrophy of muscles, cartilage, and the underlying bony structures.The disease process exhibits varying speeds of development, with onset occurring in early infancy or adolescence. Clinicians have classically reserved treatment until the end of the process. Various treatment modalities have been attempted with differing results...
September 2015: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/26044392/platelet-preparations-for-use-in-facial-rejuvenation-and-wound-healing-a-critical-review-of-current-literature
#8
REVIEW
Anthony P Sclafani, James Azzi
In facial plastic surgery, the potential for direct delivery of growth factors from platelet preparations has been of particular interest for use in facial rejuvenation, recovery after facial surgery, and wound healing. A literature search was conducted through PubMed for the terms PRP, PRFM, platelet-rich plasma, platelet-rich fibrin matrix, platelet preparations, platelet therapy, growth factors, platelet facial, platelet facial rejuvenation, platelet wound healing, platelet plastic surgery. Articles pertaining to the use of platelet preparations in facial surgery and wound healing in plastic surgery after 2001 were included...
August 2015: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/25805284/improved-fat-graft-survival-by-different-volume-fractions-of-platelet-rich-plasma-and-adipose-derived-stem-cells
#9
Feng Li, Weihua Guo, Kun Li, Mei Yu, Wei Tang, Hang Wang, Weidong Tian
BACKGROUND: The success of soft-tissue augmentation is offset by the low survival rates of grafted fat tissue. Research shows that adipose-derived stem cells (ASCs) and platelet-rich plasma (PRP) are beneficial to tissue healing. OBJECTIVES: To evaluate the long-term effects of different volume fractions of PRP combined with ASCs on fat graft. METHODS: ASCs were isolated from human fat tissue, and PRP was obtained from human blood. Cell count kit-8 and real-time polymerase chain reaction (PCR) were used to evaluate the influence of PRP (0%, 10%, 20%, and 30%; volume/volume [v/v]) in medium on ASC proliferation and adipogenic differentiation, respectively...
March 2015: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/25710554/the-effect-of-combined-use-of-platelet-rich-plasma-and-adipose-derived-stem-cells-on-fat-graft-survival
#10
Nevra Seyhan, Dogan Alhan, Ali Ugur Ural, Armagan Gunal, Mustafa Cihad Avunduk, Nedim Savaci
BACKGROUND: Free fat grafts have an unpredictable survival rate that limits their successful use. To increase the viability of fat grafts, it is important to minimize the reabsorption rate. OBJECTIVE: Our aim was to investigate whether the combined use of platelet-rich plasma (PRP) and adipose derived stem cells (ADSCs) would contribute an improvement in lower resorption rates of fat grafts. METHODS: Inbred Fischer 344 rats were randomized into 4 groups (n = 10)...
May 2015: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/25546695/preliminary-study-on-electrophysiological-changes-after-cellular-autograft-in-age-related-macular-degeneration
#11
Paolo Giuseppe Limoli, Enzo Maria Vingolo, Marco Ulisses Morales, Marcella Nebbioso, Celeste Limoli
Evolving atrophic macular degeneration represents at least 80% of all macular degenerations and is currently without a standardized care. Autologous fat transplantation efficacy was demonstrated by several studies, as these cells are able to produce growth factors. The aim of the work was to demonstrate possible therapeutic effect of the joined suprachoroidal graft of adipocytes, adipose-derived stem cells (ADSCs) in stromal vascular fractions (SVFs) of adipose tissue, and platelet-rich plasma (PRP). Twelve eyes in 12 dry age-related macular degeneration (AMD) patients, aged 71...
December 2014: Medicine (Baltimore)
https://www.readbyqxmd.com/read/25489498/platlet-rich-plasma-prp-improves-fat-grafting-outcomes
#12
REVIEW
Ali Modarressi
Autologous fat transfer offers many qualities of a ideal soft tissue filler. Main advantages of fat grafting ensue from the fact that the lipoaspirate tissue is an abundant source of regenerative pluripotential cells. However, the reported rates of fat cell survival vary greatly in the medical literature (10-90%). Different techniques of harvesting, processing, and reinjecting the fat cells are so claimed to be responsible for these differences, without any agreement concerning the best way to process. To address this important disadvantage, we propose the addition of autologous platelet rich plasma (PRP) which is known as a natural reservoir of growth factors stimulating tissue repair and regeneration...
January 2013: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/25028112/platelet-rich-plasma-mixed-fat-grafting-a-reasonable-prosurvival-strategy-for-fat-grafts
#13
REVIEW
José Maria Serra-Mestre, José Maria Serra-Renom, Lourdes Martinez, Aurora Almadori, Francesco D'Andrea
BACKGROUND: One of the most interesting developments in practical applications of fat grafting in recent years is the use of prosurvival strategies to improve maintenance of volume. Platelet-rich plasma (PRP) plays a decisive role in the repair and regeneration of different tissues via the activation and secretion of a great variety of growth factors and other cytokines stored in the alpha-granules of the platelets. This review aimed to assess the efficacy of PRP mixed-fat grafting as a prosurvival strategy for fat grafts...
October 2014: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/24984784/the-effects-of-platelet-rich-plasma-on-recovery-time-and-aesthetic-outcome-in-facial-rejuvenation-preliminary-retrospective-observations
#14
Joep C N Willemsen, Berend van der Lei, Karin M Vermeulen, Hieronymus P J D Stevens
BACKGROUND: This study focused on the possible effect of platelet-rich plasma (PRP) on recovery time and aesthetic outcome after facial rejuvenation. We conducted a retrospective analysis with regard to recovery time and the aesthetic improvement after treatment among four groups of patients: those treated with fat grafting only (Group I), those treated with fat grafting and PRP (Group II), those treated with a minimal access cranial suspension (MACS)-lift and fat grafting (Group III), and those treated with a MACS-lift, fat grafting, and PRP (Group IV)...
October 2014: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/24978937/increased-survival-of-human-free-fat-grafts-with-varying-densities-of-human-adipose-derived-stem-cells-and-platelet-rich-plasma
#15
Kun Li, Feng Li, Jie Li, Hang Wang, Xiaohui Zheng, Jie Long, Weihua Guo, Weidong Tian
The high absorption rate of transplanted fat has limited the application of autogenous fat grafts in the clinical setting. Therefore, this study aimed to evaluate the effects of platelet-rich plasma (PRP) and adipose-derived stem cells (ASCs) on fat regeneration by investigating the impact of PRP and conditioned medium on the biological characteristics of ASCs. Fat grafts were prepared with ASCs at densities of 10(7) /ml, 10(6) /ml, 10(5) /ml, 10(4) /ml and 0/ml with and without PRP and injected subcutaneously into nude mice...
June 30, 2014: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/24868255/fat-graft-laser-co%C3%A2-and-platelet-rich-plasma-synergy-in-scars-treatment
#16
A C Nita, O A Orzan, M Filipescu, D Jianu
RATIONALE: Many treatments have been proposed for cosmetic or functional improvement of scars. It is known that fat grafts and laser treatment can have beneficial effects on the remodeling of scar tissue, and platelet-rich plasma (PRP) can be effective during the wound-healing process. We hypothesized that laser and PRP can enhance fat graft survival and the combination would be effective in improving scars appearance. OBJECTIVE: The purpose of this study was to evaluate the efficacy of these combinations in the treatment of atrophic and contractile scars...
2013: Journal of Medicine and Life
https://www.readbyqxmd.com/read/24406591/adipose-derived-stromal-vascular-fraction-cells-and-platelet-rich-plasma-basic-and-clinical-evaluation-for-cell-based-therapies-in-patients-with-scars-on-the-face
#17
COMPARATIVE STUDY
Pietro Gentile, Barbara De Angelis, Methap Pasin, Giulio Cervelli, Cristiano B Curcio, Micol Floris, Camilla Di Pasquali, Ilaria Bocchini, Alberto Balzani, Fabio Nicoli, Chiara Insalaco, Eleonora Tati, Lucilla Lucarini, Ludovico Palla, Michele Pascali, Pamela De Logu, Chiara Di Segni, Davide J Bottini, Valerio Cervelli
BACKGROUND: Actually, autologous fat grafts have many clinical applications in breast surgery, facial rejuvenation, buttock augmentation, and Romberg syndrome as well as a treatment of liposuction sequelae. OBJECTIVE: The aim of this article was to describe the preparation and isolation procedures for stromal vascular fraction (SVF), the preparation of platelet-rich plasma (PRP), and the clinical application in the treatment of the scar on the face. METHODS: Ten patients with burns sequelae (n = 6) and post-traumatic scars (n = 4) were treated with SVF-enhanced autologous fat grafts obtained by the Celution System...
January 2014: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/24398999/the-synergy-between-lasers-and-adipose-tissues-surgery-in-cervicofacial-rejuvenation-histopathological-aspects
#18
Andreea Cristina Niţă, Dana Mihaela Jianu, I P Florescu, Maria Filipescu, O Cobani, S A Jianu, D A Chiriţă, Adriana Bold
BACKGROUND AND AIMS: Nowadays patients want a long-lasting youthful appearance but through a less invasive approach. Our unique approach, "AdipoLASER reJuvenation (ALJ)", involves a variety of less invasive techniques based on simultaneous laser resurfacing, and lipolysis together with adipose tissue graft and redistribution. Recently, we added to this platelet-rich plasma therapy. We conducted a study aiming to emphasize the histopathological changes occurred following these procedures...
2013: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/24273385/results-and-long-term-patient-satisfaction-after-gluteal-augmentation-with-platelet-rich-plasma-enriched-autologous-fat
#19
Joep C N Willemsen, Nicole Lindenblatt, Hieronymus P J D Stevens
BACKGROUND: Buttock augmentation is gaining increasing popularity in aesthetic surgery. The relatively high incidence of complications after silicone implant placement lead to the increased use of lipofilling techniques, yielding variable results with respect to graft take rate and long-term stability. Platelet-rich plasma (PRP) has been shown to have beneficial effects on wound healing and angiogenesis in the past. Therefore, we aimed at investigating the long-term results and patient satisfaction after PRP-enriched lipofilling for buttock augmentation...
2013: European Journal of Plastic Surgery
https://www.readbyqxmd.com/read/24191244/p-r-l-platelet-rich-lipotransfert-our-experience-and-current-state-of-art-in-the-combined-use-of-fat-and-prp
#20
REVIEW
V Cervelli, I Bocchini, C Di Pasquali, B De Angelis, G Cervelli, C B Curcio, A Orlandi, M G Scioli, E Tati, P Delogu, Pietro Gentile
The authors report their experience about the use of P.R.L. PLATELET RICH LIPOTRANSFERT method (platelet rich plasma mixed fat grafting) in 223 patients affected by soft tissue defects (ulcers, Romberg syndrome, Hemifacial atrophy, loss of substance, and signs of aging). This paper introduces the reader to PRP therapy and reviews the current literature on this emerging treatment modality, showing at the current clinical use of PRP in plastic and reconstructive surgery, with description of innovative methods and future prospects...
2013: BioMed Research International
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