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Hongbo Chen, Yinan Yao, Yifan Wang, Hua Zhou, Tianxiang Xu, Jing Liu, Guocheng Wang, Yongfeng Zhang, Xiang Chen, Qingwei Liu, Peng Huang, Rongbin Yu
BACKGROUND: HLA-DM gene, which is related to antigen processing and presentation and located in the non-classical class-II region of human leukocyte antigen (HLA) region, may play a crucial role in chronic hepatitis C virus (HCV) infection treatment outcomes. The study was conducted to evaluate the role of the variant of several single nucleotide polymorphisms (SNPs) in HLA-DM gene in HCV treatment outcomes. METHODS: We genotyped four SNPs from the candidate genes (HLA-DMA and DMB) in 336 patients who were treated with pegylated interferon-alpha and ribavirin (PEG IFN-α/RBV)...
October 20, 2016: International Journal of Environmental Research and Public Health
Theodoric A Mattes, Jorge C Escalante-Semerena
5,6-Dimethylbenzimidazolyl-(DMB)-α-ribotide, [α-ribazole-5'-phosphate (α-RP)] is an intermediate in the biosynthesis of adenosylcobalamin (AdoCbl) in many prokaryotes. In such microbes, α-RP is synthesized by nicotinate mononucleotide (NaMN):DMB phosphoribosyltransferases (CobT in S. enterica), in a reaction that is considered to be the canonical step for the activation of the base of the nucleotide present in adenosylcobamides. Some Firmicutes lack CobT-type enzymes, but have a two-protein system comprised of a transporter (i...
October 17, 2016: Molecular Microbiology
Caili Fu, Chang Liu, Ying Li, Yajing Guo, Fang Luo, Peilong Wang, Longhua Guo, Bin Qiu, Zhenyu Lin
Abasic site (AP site) in the triplex structure can recognize specific target with high selectivity. In this study, this character was first applied to develop a simple, sensitive, and selective homogeneous electrochemical biosensor for melamine determination. The assay combines the advantage of the high selectivity of the DNA triplex structure containing an AP site to melamine and high efficiency of exonuclease (Exo) III-assisted recycling amplification. DNA-1 (T1), DNA-2 (T2), poly[dA] probe containing an AP site (8A) and methylene blue-labeled DNA probe (dMB probe) were carefully designed...
October 3, 2016: Analytical Chemistry
N Gottschall, E Topp, M Edwards, M Payne, S Kleywegt, D R Lapen
Dewatered municipal biosolids (DMB) were applied at a rate of 22Mgdwha(-1) to an agricultural field in fall 2008. Concentrations of polybrominated diphenyl ethers (PBDEs; BDE-47, -99, -100, -153, -154, -183, -197, -207, -209), other brominated flame retardants (BFRs; HBB, PBEB, DBDPE, BTBPE) and perfluoroalkyl acids (PFAAs; PFHxS, PFOS, PFDS, PFOSA, PFHpA, PFOA, PFNA, PFDA, PFUnA, PFDoA, PFTA) were monitored in tile drainage, groundwater (2m, 4m and 6m depth), soil cores (0-0.3m) pre- and post-application, DMB aggregates incorporated into the soil post-application, and in wheat (Triticum spp...
September 16, 2016: Science of the Total Environment
Rahulkumar Maurya, Tonmoy Ghosh, Hitesh Saravaia, Chetan Paliwal, Arup Ghosh, Sandhya Mishra
Non-isothermal (β=5, 10, 20, 35°C/min) pyrolysis of de-oiled microalgal biomass (DMB) of Chlorella variabilis was investigated by TGA-MS (30-900°C, Argon atmosphere) to understand thermal decomposition and evolved gas analysis (EGA). The results showed that three-stage thermal decomposition and three volatilization zone (100-400°C, 400-550°C and 600-750°C) of organic matters during pyrolysis. The highest rate of weight-loss is 8.91%/min at 302°C for 35°C/min heating-rate. Kinetics of pyrolysis were investigated by iso-conversional (KAS, FWO) and model-fitting (Coats-Redfern) method...
December 2016: Bioresource Technology
Kate H Dinh, Ariana F Harris, Jennifer LaFemina, Giles F Whalen, Mary Sullivan, Robert Licho, Thomas Hill, Laura A Lambert
BACKGROUND AND OBJECTIVES: Lymphatic mapping (LM) and blue dye injections are essential to identification of sentinel lymph nodes (SLN) for melanoma. LM is performed the day before (DB) or the same day (SD) of surgery, but the optimal timing is unknown. Similarly, methylene blue (MB), used during SLN biopsy (SLNB), is administered diluted (dMB) or undiluted (uMB), but the relative efficacies are unknown. METHODS: Patients who underwent SLNB for melanoma from 2009 to 2013 at our institution were evaluated...
September 16, 2016: Journal of Surgical Oncology
Yata Praveen Kumar, C Shobha Devi, A Srishailam, N Deepika, V Ravi Kumar, P Venkat Reddy, K Nagasuryaprasad, Surya S Singh, Penumaka Nagababu, S Satyanarayana
This article describes the synthesis and characterization of three new Ru(II) polypyridyl complexes including [Ru(phen)2(dpphz)](2+) (1), [Ru(bpy)2(dpphz)](2+) (2) and [Ru(dmb)2(dpphz)](2+) (3) where dpphz = dipyrido[3,2-a:2',3'-c] phenazine-11-hydrazide, phen =1,10-phenanthroline, bpy = 2,2'-bipyridine and dmb = 4,4'-dimethyl2,2'-bipyridine. The binding behaviors of these complexes to calf thymus DNA (CT-DNA) were explored by spectroscopic titrations, viscosity measurements. Results suggest that these complexes can bind to CT-DNA through intercalation...
September 2, 2016: Journal of Fluorescence
Christiane Auray-Blais, Pamela Lavoie, Shunji Tomatsu, Vassili Valayannopoulos, John J Mitchell, Julian Raiman, Maxime Beaudoin, Bruno Maranda, Joe T R Clarke
Mucopolysaccharidoses (MPSs) are a group of disorders resulting from primary defects in lysosomal enzymes involved in the degradation of glycosaminoglycans (GAGs). Depending on the specific enzyme defect, the catabolism of one or more GAGs is blocked leading to accumulation in tissues and biological fluids. GAG measurements are important for high-risk screening, diagnosis, monitoring treatment efficacy, and patient follow up. The dimethylmethylene blue (DMB) spectrophotometric method commonly used in most biochemical genetics laboratories relies on a non-specific total GAG analysis which has led to false positive results, and even false negative results (mainly for MPS III and IV patients)...
September 14, 2016: Analytica Chimica Acta
Yusuke Aihara, Kyohei Sato, Kazuteru Shinozaki
We synthesized neutral Ru(II) complexes cis-Ru(bpy)2(CN)2 (bpy = 2,2'-bipyridine), cis-Ru(dmb)2(CN)2 (dmb = 4,4'-dimethyl-2,2'-bipyridine), cis-Ru(dbb)2(CN)2 (dbb = 4,4'-di-tert-butyl-2,2'-bipyridine), and cis-Ru(phen)2(CN)2 (phen = 1,10-phenanthroline) and optically resolved them into respective enantiomers using high-performance liquid chromatography with a chiral column. The absolute configuration of enantiomer of cis-Ru(dbb)2(CN)2 was determined by an X-ray crystallography. Upon photoirradiation, the entire enantiomers of the complexes underwent the racemization with considerably slow rates (k = 1 × 10(-6) to 1 × 10(-5) s(-1)) and small quantum yields (ϕ = 1 × 10(-6) to 1 × 10(-5))...
September 6, 2016: Inorganic Chemistry
Sara M Elkashef, Mark Sutherland, Laurence H Patterson, Paul M Loadman, Robert A Falconer
The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.
October 21, 2016: Analyst
Claudio Chiastra, Francesco Iannaccone, Maik J Grundeken, Frank J H Gijsen, Patrick Segers, Matthieu De Beule, Patrick W Serruys, Joanna J Wykrzykowska, Antonius F W van der Steen, Jolanda J Wentzel
BACKGROUND: Coronary hemodynamics and physiology specific for bifurcation lesions was not well understood. To investigate the influence of the bifurcation angle on the intracoronary hemodynamics of side branch (SB) lesions computational fluid dynamics simulations were performed. METHODS: A parametric model representing a left anterior descending-first diagonal coronary bifurcation lesion was created according to the literature. Diameters obeyed fractal branching laws...
2016: Biomedical Engineering Online
Lara Massai, Damiano Cirri, Elena Michelucci, Gianluca Bartoli, Annalisa Guerri, Maria A Cinellu, Fabio Cocco, Chiara Gabbiani, Luigi Messori
In the last few years gold(III) complexes have attracted growing attention in the medicinal chemistry community as candidate anticancer agents. In particular some organogold(III) compounds manifested quite attractive pharmacological behaviors in preclinical studies. Here we compare the chemical and biological properties of the novel organogold(III) complex [Au(bipy(dmb)-H)(NH(CO)CH3)][PF6] (Aubipy(aa)) with those of its parent compounds [Au(bipy(dmb)-H)(OH)][PF6] (Aubipy(c)) and [Au2(bipy(dmb)-H)2)(μ-O)][PF6]2 (Au2bipy(c)), previously synthesized and characterized...
October 2016: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Miao-Miao Xue, Chen-Chao Huang, Yi Yuan, Lin-Song Cui, Yong-Xi Li, Bo Wang, Zuo-Quan Jiang, Man-Keung Fung, Liang-Sheng Liao
Borane is an excellent electron-accepting species, and its derivatives have been widely used in a variety of fields. However, the use of borane derivatives as host materials in OLEDs has rarely reported because the device performance is generally not satisfactory. In this work, two novel spiro-bipolar hosts with incorporated borane were designed and synthesized. The strategies used in preparing these materials were to increase the spatial separation of the highest occupied molecular orbitals (HOMOs) and lowest unoccupied molecular orbitals (LUMOs) in the molecules, tune the connecting positions of functional groups, and incorporate specific functional groups with desirable thermal stability...
August 10, 2016: ACS Applied Materials & Interfaces
Marissa R Civic, Peter H Dinolfo
Electrochemical charge transfer through multilayer thin films of zinc and nickel 5,10,15,20-tetra(4-ethynylphenyl) porphyrin constructed via copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) "click" chemistry was examined. Current rectification toward various outer-sphere redox probes is revealed with increasing numbers of layers, as these films possess insulating properties over the neutral potential range of the porphyrin, then become conductive upon reaching its oxidation potential. Interfacial electron transfer rates of mediator-dye interactions toward [Co(bpy)3](2+), [Co(dmb)3](2+), [Co(NO2-phen)3](2+), [Fe(bpy)3](2+), and ferrocene (Fc), all outer-sphere redox species, were measured by hydrodynamic methods...
August 10, 2016: ACS Applied Materials & Interfaces
Peer Schrapers, Stefan Mebs, Sebastian Goetzl, Sandra E Hennig, Holger Dau, Holger Dobbek, Michael Haumann
A cobalamin (Cbl) cofactor in corrinoid iron-sulfur protein (CoFeSP) is the primary methyl group donor and acceptor in biological carbon oxide conversion along the reductive acetyl-CoA pathway. Changes of the axial coordination of the cobalt ion within the corrin macrocycle upon redox transitions in aqua-, methyl-, and cyano-Cbl bound to CoFeSP or in solution were studied using X-ray absorption spectroscopy (XAS) at the Co K-edge in combination with density functional theory (DFT) calculations, supported by metal content and cobalt redox level quantification with further spectroscopic methods...
2016: PloS One
Yongchen Wang, Zheng Zhao, Yan Yan, Xiaoyan Qiang, Cuisong Zhou, Ruiyan Li, Huan Chen, Yubin Zhang
Demethyleneberberine (DMB) is an essential metabolite of Berberine (BBR) in vivo. Recent reports have revealed multiple novel therapeutic applications of BBR. However, the pharmacological activities of DMB remain to be elucidated. This study aimed to demonstrate the hepatoprotective and anti-fibrotic effects of DMB both in vitro and in vivo. Here we showed that DMB protects against thioacetamide (TAA)-induced hepatic fibrosis in mice and exhibits a higher safety profile as compared to BBR. Flow cytometry and Western blotting analysis showed that DMB is able to suppress the activation of hepatic stellate cells (HSCs) and induce cell apoptosis through the nuclear factor-κB (NF-κB) cascade...
2016: International Journal of Molecular Sciences
Hong-Xiu Diao, Shuai Zhang, Xue-Yuan Hu, Wei Guan, Li Luan, Hai-Yu Liu, Hong-Gang Fan
OBJECTIVE: To evaluate the behavior and some cardiopulmonary variables of dexmedetomidine-midazolam or dexmedetomidine-midazolam-butorphanol in the silver fox (Vulpes vulpes). STUDY DESIGN: Blinded, randomized design. ANIMALS: Sixteen adult silver foxes, aged 7-9 months, weighing 6.0-9.2 kg. METHODS: Animals were randomly assigned to dexmedetomidine (50 μg kg(-1) ) and midazolam (0.45 mg kg(-1) ) (group DM) or to dexmedetomidine (30 μg kg(-1) ), midazolam (0...
June 28, 2016: Veterinary Anaesthesia and Analgesia
Chuan-Chuan Zeng, Shang-Hai Lai, Jun-Hua Yao, Cheng Zhang, Hui Yin, Wei Li, Bing-Jie Han, Yun-Jun Liu
Four new ruthenium(II) polypyridyl complexes [Ru(N-N)2(dhbn)](ClO4)2 (N-N = dmb: 4,4'-dimethyl-2,2'-bipyridine 1; bpy = 2,2'-bipyridine 2; phen = 1,10-phenanthroline 3; dmp = 2,9-dimethyl-1,10-phenanthroline 4) were synthesized and characterized. The cytotoxicity in vitro of the ligand and complexes toward HepG-2, HeLa, MG-63 and A549 were assayed by MTT method. The IC50 values of the complexes against the above cells range from 17.7 ± 1.1 to 45.1 ± 2.8 μM. The cytotoxic activity of the complexes against HepG-2 cells follows the order of 4 > 2 > 3 > 1...
October 21, 2016: European Journal of Medicinal Chemistry
Ryuichi Mashima, Eri Sakai, Misa Tanaka, Motomichi Kosuga, Torayuki Okuyama
Glycosaminoglycans (GAGs) play important roles on the regulation of extracellular signaling, neuronal development, and cartilage maintenance. The extracellular concentration of total GAGs has been used as an established measure for the diagnosis of mucopolysaccharidoses (MPSs). Heparan sulfate (HS), Dermatan sulfate (DS) and chondroitin sulfate are known to be elevated in the GAGs under pathological conditions associated with MPS. Furthermore, the selective accumulation of disease-specific one of, or a combination of, them has also been used for the estimation of subtypes of MPS...
June 2016: Molecular Genetics and Metabolism Reports
Junheon Kim, Miyeon Jang, Kyoung-Tae Lee, Kyungjae Andrew Yoon, Chung Gyoo Park
Drosophila suzukii is an economically important pest in America and Europe as well as in Asia. Sparassol and methyl orsellinate are naturally produced by the cultivating mushrooms Sparassis cripta and Sparassis latifolia. Fumigant and contact toxicities of synthetic sparassol and its analogues, methyl orsellinate and methyl 2,4-dimethoxy-6-methylbenzoate (DMB), were investigated. Negligible fumigant activity was observed from the tested compounds. However, DMB showed the strongest contact toxicity, followed by sparassol and methyl orsellinate...
July 13, 2016: Journal of Agricultural and Food Chemistry
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