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Fibronectin assembly

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https://www.readbyqxmd.com/read/28327179/pterostilbene-prevents-akt-erk-axis-mediated-polymerization-of-surface-fibronectin-on-suspended-lung-cancer-cells-independently-of-apoptosis-and-suppresses-metastasis
#1
Ying-Jan Wang, Jing-Fang Lin, Li-Hsin Cheng, Wen-Tsan Chang, Ying-Hsien Kao, Ming-Min Chang, Bour-Jr Wang, Hung-Chi Cheng
BACKGROUND: Polymeric fibronectin (polyFN) assembled on suspended breast cancer cells is required for metastasis. Conceivably, drugs that target such polyFN may fight against cancer metastasis. While stilbene analogs trigger pro-apoptotic effect on attached cancer cells, whether they prevent polyFN assembly and metastasis of suspended cancer cells via an apoptosis-independent manner remains unexplored. METHODS: We depleted suspended Lewis lung carcinoma (LLC) cells of polyFN by silencing the endogenous FN expression or pterostilbene (PS) to examine whether metastasis of lung cancer cells could thus be suppressed...
March 21, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28316141/dasatinib-inhibits-actin-fiber-reorganization-and-promotes-endothelial-cell-permeability-through-rhoa-rock-pathway
#2
Swapan K Dasgupta, Anhquyen Le, K Vinod Vijayan, Perumal Thiagarajan
Treatment with dasatinib, a tyrosine kinase inhibitor, is associated with edema, pleural effusion, and pulmonary edema. We investigated the effect of dasatinib on the barrier function of human microvascular endothelial cells-1 (HMEC-1) in vitro and in vivo. The permeability of HMEC-1 to fluorescein isothiocyante (FITC)-dextran increased in Transwell chambers within 5 min following the addition of therapeutic concentrations of dasatinib. The change in permeability was associated with increased activation of RhoA GTPase and its effector Rho-associated coiled-coil kinase 1(ROCK1)...
March 18, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28279265/engineered-microenvironments-for-synergistic-vegf-integrin-signalling-during-vascularization
#3
Vladimíra Moulisová, Cristina Gonzalez-García, Marco Cantini, Aleixandre Rodrigo-Navarro, Jessica Weaver, Mercedes Costell, Roser Sabater I Serra, Matthew J Dalby, Andrés J García, Manuel Salmerón-Sánchez
We have engineered polymer-based microenvironments that promote vasculogenesis both in vitro and in vivo through synergistic integrin-growth factor receptor signalling. Poly(ethyl acrylate) (PEA) triggers spontaneous organization of fibronectin (FN) into nanonetworks which provide availability of critical binding domains. Importantly, the growth factor binding (FNIII12-14) and integrin binding (FNIII9-10) regions are simultaneously available on FN fibrils assembled on PEA. This material platform promotes synergistic integrin/VEGF signalling which is highly effective for vascularization events in vitro with low concentrations of VEGF...
May 2017: Biomaterials
https://www.readbyqxmd.com/read/28273941/arterial-graft-with-elastic-layer-structure-grown-from-cells
#4
Utako Yokoyama, Yuta Tonooka, Ryoma Koretake, Taisuke Akimoto, Yuki Gonda, Junichi Saito, Masanari Umemura, Takayuki Fujita, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, Yoshihiro Ishikawa
Shortage of autologous blood vessel sources and disadvantages of synthetic grafts have increased interest in the development of tissue-engineered vascular grafts. However, tunica media, which comprises layered elastic laminae, largely determines arterial elasticity, and is difficult to synthesize. Here, we describe a method for fabrication of arterial grafts with elastic layer structure from cultured human vascular SMCs by periodic exposure to extremely high hydrostatic pressure (HP) during repeated cell seeding...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28257580/a-novel-antifibrotic-mechanism-of-nintedanib-and-pirfenidone-inhibition-of-collagen-fibril-assembly
#5
Larissa Knüppel, Yoshihiro Ishikawa, Michaela Aichler, Katharina Heinzelmann, Rudolf Hatz, Jürgen Behr, Axel Walch, Hans Peter Bächinger, Oliver Eickelberg, Claudia A Staab-Weijnitz
RATIONALE: Idiopathic pulmonary fibrosis (IPF) is characterized by excessive deposition of extracellular matrix, in particular collagens. Two IPF therapeutics, nintedanib and pirfenidone, decelerate lung function decline, but their underlying mechanisms of action are poorly understood. In this study we sought to analyze their effects on collagen synthesis and maturation at important regulatory levels. METHODS: Primary human fibroblasts from IPF patients and healthy donors were treated with nintedanib (0...
March 3, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28230931/spatially-assembled-bilayer-cell-sheets-of-stem-cells-and-endothelial-cells-using-thermosensitive-hydrogels-for-therapeutic-angiogenesis
#6
Indong Jun, Taufiq Ahmad, Seongwoo Bak, Joong-Yup Lee, Eun Mi Kim, Jinkyu Lee, Yu Bin Lee, Hongsoo Jeong, Hojeong Jeon, Heungsoo Shin
Although the coculture of multiple cell types has been widely employed in regenerative medicine, in vivo transplantation of cocultured cells while maintaining the hierarchical structure remains challenging. Here, a spatially assembled bilayer cell sheet of human mesenchymal stem cells and human umbilical vein endothelial cells on a thermally expandable hydrogel containing fibronectin is prepared and its effect on in vitro proangiogenic functions and in vivo ischemic injury is investigated. The expansion of hydrogels in response to a temperature change from 37 to 4 °C allows rapid harvest and delivery of the bilayer cell sheet to two different targets (an in vitro model glass surface and in vivo tissue)...
February 23, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28193541/a-nano-scaled-and-multi-layered-recombinant-fibronectin-cadherin-chimera-composite-selectively-concentrates-osteogenesis-related-cells-and-factors-to-aid-bone-repair
#7
Junchao Xing, Tieniu Mei, Keyu Luo, Zhiqiang Li, Aijun Yang, Zhilin Li, Zhao Xie, Zehua Zhang, Shiwu Dong, Tianyong Hou, Jianzhong Xu, Fei Luo
Easily accessible and effective bone grafts are in urgent need in clinic. The selective cell retention (SCR) strategy, by which osteogenesis-related cells and factors are enriched from bone marrow into bio-scaffolds, holds great promise. However, the retention efficacy is limited by the relatively low densities of osteogenesis-related cells and factors in marrow; in addition, a lack of satisfactory surface modifiers for scaffolds further exacerbates the dilemma. To address this issue, a multi-layered construct consisting of a recombinant fibronectin/cadherin chimera was established via a layer-by-layer self-assembly technique (LBL-rFN/CDH) and used to modify demineralised bone matrix (DBM) scaffolds...
February 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28192862/long-term-dietary-quercetin-enrichment-as-a-cardioprotective-countermeasure-in-mdx-mice
#8
Christopher Ballmann, Thomas Denney, Ronald J Beyers, Tiffany Quindry, Matthew Romero, Joshua T Selsby, John C Quindry
Duchenne Muscular Dystrophy (DMD) causes declines in cardiac health resulting in premature mortality. As a potential countermeasure, quercetin is a polyphenol possessing inherent anti-inflammatory and antioxidant effects that activate proliferator-activated γ coactivator 1α (PGC-1α) increasing mitochondrial biogenesis protein abundance. We investigated the extent to which lifelong 0.2% dietary quercetin enrichment attenuates dystrophic cardiopathology in mdx mice. Dystrophic animals were fed quercetin or control diet for 12 months while control C57 mice were fed a control diet...
February 13, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28192654/self-assembly-of-recombinant-silk-as-a-strategy-for-chemical-free-formation-of-bioactive-coatings-a-real-time-study
#9
Linnea Nilebäck, Jesper Hedin, Mona Widhe, Lotta S Floderus, Annika Krona, Helena Bysell, My Hedhammar
Functionalization of biomaterials with biologically active peptides can improve their performance after implantation. By genetic fusion to self-assembling proteins, the functional peptides can easily be presented on different physical formats. Herein, a chemical-free coating method based on self-assembly of the recombinant spider silk protein 4RepCT is described and used to prepare functional coatings on various biomaterial surfaces. The silk assembly was studied in real-time, revealing the occurrence of continuous assembly of silk proteins onto surfaces and the formation of nanofibrillar structures...
March 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28163239/enhanced-elastin-synthesis-and-maturation-in-human-vascular-smooth-muscle-tissue-derived-from-induced-pluripotent-stem-cells
#10
Joon H Eoh, Nian Shen, Jacqueline A Burke, Svenja Hinderer, Zhiyong Xia, Katja Schenke-Layland, Sharon Gerecht
Obtaining vascular smooth muscle tissue with mature, functional elastic fibers is a key obstacle in tissue-engineered blood vessels. Poor elastin secretion and organization leads to a loss of specialization in contractile smooth muscle cells, resulting in over proliferation and graft failure. In this study, human induced-pluripotent stem cells (hiPSCs) were differentiated into early smooth muscle cells, seeded onto a hybrid poly(ethylene glycol) dimethacrylate/poly (L-lactide) (PEGdma-PLA) scaffold and cultured in a bioreactor while exposed to pulsatile flow, towards maturation into contractile smooth muscle tissue...
February 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28159817/tissue-transglutaminase-modulates-vascular-stiffness-and-function-through-crosslinking-dependent-and-crosslinking-independent-functions
#11
Jochen Steppan, Yehudit Bergman, Kayla Viegas, Dinani Armstrong, Siqi Tan, Huilei Wang, Sean Melucci, Daijiro Hori, Sung Yong Park, Sebastian F Barreto, Abraham Isak, Sandeep Jandu, Nicholas Flavahan, Mark Butlin, Steven S An, Alberto Avolio, Dan E Berkowitz, Marc K Halushka, Lakshmi Santhanam
BACKGROUND: The structural elements of the vascular wall, namely, extracellular matrix and smooth muscle cells (SMCs), contribute to the overall stiffness of the vessel. In this study, we examined the crosslinking-dependent and crosslinking-independent roles of tissue transglutaminase (TG2) in vascular function and stiffness. METHODS AND RESULTS: SMCs were isolated from the aortae of TG2-/- and wild-type (WT) mice. Cell adhesion was examined by using electrical cell-substrate impedance sensing and PicoGreen assay...
February 3, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28143748/a-three-dimensional-neural-spheroid-model-for-capillary-like-network-formation
#12
Molly E Boutin, Liana L Kramer, Liane L Livi, Tyler Brown, Christopher Moore, Diane Hoffman-Kim
BACKGROUND: In vitro three-dimensional neural spheroid models have an in vivo-like cell density, and have the potential to reduce animal usage and increase experimental throughput. The aim of this study was to establish a spheroid model to study the formation of capillary-like networks in a three-dimensional environment that incorporates both neuronal and glial cell types, and does not require exogenous vasculogenic growth factors. NEW METHOD: We created self-assembled, scaffold-free cellular spheroids using primary-derived postnatal rodent cortex as a cell source...
January 29, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28128308/%C3%AE-v-class-integrins-exert-dual-roles-on-%C3%AE-5%C3%AE-1-integrins-to-strengthen-adhesion-to-fibronectin
#13
Mitasha Bharadwaj, Nico Strohmeyer, Georgina P Colo, Jonne Helenius, Niko Beerenwinkel, Herbert B Schiller, Reinhard Fässler, Daniel J Müller
Upon binding to the extracellular matrix protein, fibronectin, αV-class and α5β1 integrins trigger the recruitment of large protein assemblies and strengthen cell adhesion. Both integrin classes have been functionally specified, however their specific roles in immediate phases of cell attachment remain uncharacterized. Here, we quantify the adhesion of αV-class and/or α5β1 integrins expressing fibroblasts initiating attachment to fibronectin (≤120 s) by single-cell force spectroscopy. Our data reveals that αV-class integrins outcompete α5β1 integrins...
January 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28120882/haemodynamics-regulate-fibronectin-assembly-via-pecam
#14
Zhongming Chen, Chris Givens, John S Reader, Ellie Tzima
Fibronectin (FN) assembly and fibrillogenesis are critically important in both development and the adult organism, but their importance in vascular functions is not fully understood. Here we identify a novel pathway by which haemodynamic forces regulate FN assembly and fibrillogenesis during vascular remodelling. Induction of disturbed shear stress in vivo and in vitro resulted in complex FN fibril assembly that was dependent on the mechanosensor PECAM. Loss of PECAM also inhibited the cell-intrinsic ability to remodel FN...
January 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28109813/amyloid-like-aggregates-formation-by-blood-plasma-fibronectin
#15
Rümeyza Bascetin, Khadija Admane, Rémy Agniel, Thomas Boudou, Tristan Doussineau, Rodolphe Antoine, Olivier Gallet, Johanne Leroy-Dudal, Charlotte Vendrely
Fibronectin (FN) is a multifunctional glycoprotein of the extracellular matrix (ECM) playing critical roles in physiological and pathological cell processes like adhesion, migration, growth, and differentiation. These various functions of FN are modulated by its supramolecular state. Indeed, FN can polymerize into different types of assemblies like fibrils and aggregates. However, the mechanism of polymerization and the effects of such assemblies on cell behaviors still remain to be elucidated. Here we show that upon irreversible thermal denaturation, human blood plasma fibronectin forms high molecular weight aggregates...
April 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109697/fibronectin-fibrils-regulate-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition
#16
Lauren A Griggs, Nadiah T Hassan, Roshni S Malik, Brian P Griffin, Brittany A Martinez, Lynne W Elmore, Christopher A Lemmon
Epithelial-Mesenchymal Transition (EMT) is a dynamic process through which epithelial cells transdifferentiate from an epithelial phenotype into a mesenchymal phenotype. Previous studies have demonstrated that both mechanical signaling and soluble growth factor signaling facilitate this process. One possible point of integration for mechanical and growth factor signaling is the extracellular matrix. Here we investigate the role of the extracellular matrix (ECM) protein fibronectin (FN) in this process. We demonstrate that inhibition of FN fibrillogenesis blocks activation of the Transforming Growth Factor-Beta (TGF-β) signaling pathway via Smad2 signaling, decreases cell migration and ultimately leads to inhibition of EMT...
January 19, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28102238/fibronectin-guided-migration-of-carcinoma-collectives
#17
Sandeep Gopal, Laurence Veracini, Dominique Grall, Catherine Butori, Sébastien Schaub, Stéphane Audebert, Luc Camoin, Emilie Baudelet, Agata Radwanska, Stéphanie Beghelli-de la Forest Divonne, Shelia M Violette, Paul H Weinreb, Samah Rekima, Marius Ilie, Anne Sudaka, Paul Hofman, Ellen Van Obberghen-Schilling
Functional interplay between tumour cells and their neoplastic extracellular matrix plays a decisive role in malignant progression of carcinomas. Here we provide a comprehensive data set of the human HNSCC-associated fibroblast matrisome. Although much attention has been paid to the deposit of collagen, we identify oncofetal fibronectin (FN) as a major and obligate component of the matrix assembled by stromal fibroblasts from head and neck squamous cell carcinomas (HNSCC). FN overexpression in tumours from 435 patients corresponds to an independent unfavourable prognostic indicator...
January 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28030649/whole-genome-sequence-and-comparative-genomics-of-the-novel-lyme-borreliosis-causing-pathogen-borrelia-mayonii
#18
Luke C Kingry, Dhwani Batra, Adam Replogle, Lori A Rowe, Bobbi S Pritt, Jeannine M Petersen
Borrelia mayonii, a Borrelia burgdorferi sensu lato (Bbsl) genospecies, was recently identified as a cause of Lyme borreliosis (LB) among patients from the upper midwestern United States. By microscopy and PCR, spirochete/genome loads in infected patients were estimated at 105 to 106 per milliliter of blood. Here, we present the full chromosome and plasmid sequences of two B. mayonii isolates, MN14-1420 and MN14-1539, cultured from blood of two of these patients. Whole genome sequencing and assembly was conducted using PacBio long read sequencing (Pacific Biosciences RSII instrument) followed by hierarchical genome-assembly process (HGAP)...
2016: PloS One
https://www.readbyqxmd.com/read/28024362/controlled-sub-nanometer-epitope-spacing-in-a-three-dimensional-self-assembled-peptide-hydrogel
#19
E Thomas Pashuck, Benoît J R Duchet, Catherine S Hansel, Stephanie A Maynard, Lesley W Chow, Molly M Stevens
Cells in the body use a variety of mechanisms to ensure the specificity and efficacy of signal transduction. One way that this is achieved is through tight spatial control over the position of different proteins, signaling sequences, and biomolecules within and around cells. For instance, the extracellular matrix protein fibronectin presents RGDS and PHSRN sequences that synergistically bind the α5β1 integrin when separated by 3.2 nm but are unable to bind when this distance is >5.5 nm.1 Building biomaterials to controllably space different epitopes with subnanometer accuracy in a three-dimensional (3D) hydrogel is challenging...
December 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/28005397/tns1-is-essential-for-myofibroblast-differentiation-and-extracellular-matrix-formation
#20
Ksenija Bernau, Elizabeth E Torr, Michael D Evans, Jason K Aoki, Caitlyn R Ngam, Nathan Sandbo
Myofibroblasts, the primary effector cells that mediate matrix remodeling during pulmonary fibrosis, rapidly assemble an extracellular fibronectin matrix1. Tensin1 (TNS1) is a key component of specialized cellular adhesions (fibrillar adhesions) that bind to extracellular fibronectin fibrils. We hypothesized that TNS1 may play a role in modulating myofibroblast-mediated matrix formation. We found that TNS1 expression is increased in fibroblastic foci from lungs with idiopathic pulmonary fibrosis. TGF-β profoundly upregulates TNS1 expression with kinetics that parallel the expression of the myofibroblast marker, ACTA2...
December 22, 2016: American Journal of Respiratory Cell and Molecular Biology
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