keyword
https://read.qxmd.com/read/38413863/rapid-visual-label-based-biosensor-platform-for-identification-of-hepatitis-c-virus-in-clinical-applications
#21
JOURNAL ARTICLE
Yuanfang Shi, Qingxue Zhou, Shilei Dong, Qi Zhao, Xue Wu, Peng Yang, Xiaoyan Zeng, Xinggui Yang, Yan Tan, Xinhua Luo, Zhenghua Xiao, Xu Chen
OBJECTIVES: In the current study, for the first time, we reported a novel HCV molecular diagnostic approach termed reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticles-based lateral flow biosensor (RT-LAMP-AuNPs-LFB), which we developed for rapid, sensitive, specific, simple, and visual identification of HCV. METHODS: A set of LAMP primer was designed according to 5'untranslated region (5'UTR) gene from the major HCV genotypes 1b, 2a, 3b, 6a, and 3a, which are prevalent in China...
February 28, 2024: BMC Microbiology
https://read.qxmd.com/read/38411083/identification-of-bacterial-dissimilatory-antimonate-reductase-anra-genes-and-proteins-involved-in-antimonate-respiration-and-resistance-in-geobacter-sp-strain-svr
#22
JOURNAL ARTICLE
Ryoya Kambara, Shigeki Yamamura, Seigo Amachi
Geobacter sp. strain SVR uses antimonate [Sb(V)] as a terminal electron acceptor for anaerobic respiration. Here, we visualized a possible key enzyme, periplasmic Sb(V) reductase (Anr), via active staining and non-denaturing gel electrophoresis. Liquid chromatography-tandem mass spectrometry analysis revealed that a novel dimethyl sulfoxide (DMSO) reductase family protein, WP_173201954.1, is involved in Anr. This protein was closely related with AnrA, a protein suggested to be the catalytic subunit of a respiratory Sb(V) reductase in Desulfuribacillus stibiiarsenatis ...
February 27, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38410722/bibliometric-analysis-of-antimalarial-drug-resistance
#23
REVIEW
Jialu Zhang, Muhammad Shahbaz, Muhammad Ijaz, Huimin Zhang
BACKGROUND: Malaria has always been a serious infectious disease prevalent in the world. Antimalarial drugs such as chloroquine and artemisinin have been the main compounds used to treat malaria. However, the massive use of this type of drugs accelerates the evolution and spread of malaria parasites, leading to the development of resistance. A large number of related data have been published by researchers in recent years. CiteSpace software has gained popularity among us researchers in recent years, because of its ability to help us obtain the core information we want in a mass of articles...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38407238/three-dimensional-morphogenesis-in-canine-gut-on-a-chip-using-intestinal-organoids-derived-from-inflammatory-bowel-disease-patients
#24
JOURNAL ARTICLE
Itsuma Nagao, Meg Nakazawa, Yoko M Ambrosini
Canine intestines possess similarities in anatomy, microbiology, and physiology to those of humans, and dogs naturally develop spontaneous intestinal disorders similar to humans. Overcoming the inherent limitation of three-dimensional (3D) organoids in accessing the apical surface of the intestinal epithelium has led to the generation of two-dimensional (2D) monolayer cultures, which expose the accessible luminal surface using cells derived from the organoids. The integration of these organoids and organoid-derived monolayer cultures into a microfluidic Gut-on-a-Chip system has further evolved the technology, allowing for the development of more physiologically relevant dynamic in vitro intestinal models...
February 9, 2024: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/38405363/unveiling-aromas-virtual-reality-and-scent-identification-for-sensory-analysis
#25
JOURNAL ARTICLE
Abdul Hannan Bin Zulkarnain, Dalma Radványi, Dorina Szakál, Zoltán Kókai, Attila Gere
Sensory analysis is crucial for optimizing experiences in various fields, including food, cosmetics, and product design. Traditional methods can be inefficient and imprecise. This study introduces a novel approach by blending Virtual Reality (VR) technology with scent identification techniques. The aim is to investigate whether the visual representation of food products affects scent perception. Limited research has explored the use of VR in scent identification, which is especially relevant when altering the food environment setting...
2024: Current research in food science
https://read.qxmd.com/read/38399808/select-cover-crop-residue-and-soil-microbiomes-contribute-to-suppression-of-fusarium-root-and-crown-rot-in-barley-and-soybean
#26
JOURNAL ARTICLE
Harini S Aiyer, Andrew McKenzie-Gopsill, Aaron Mills, Adam John Foster
Fusarium root and crown rot (FRCR) negatively impact several economically important plant species. Cover crops host different soil and residue microbiomes, thereby potentially influencing pathogen load and disease severity. The carryover effect of cover crops on FRCR in barley and soybean was investigated. Field trials were conducted in Prince Edward Island, Canada. Two cover crops from each plant group, including forbs, brassicas, legumes, and grasses, were grown in a randomized complete block design with barley and soybean planted in split plots the following year...
February 17, 2024: Microorganisms
https://read.qxmd.com/read/38396332/overcoming-the-egress-block-of-plasmodium-sporozoites-expressing-fluorescently-tagged-circumsporozoite-protein
#27
JOURNAL ARTICLE
Carolina Thieleke-Matos, Kevin Walz, Friedrich Frischknecht, Mirko Singer
Plasmodium sporozoites are the highly motile and invasive forms of the malaria parasite transmitted by mosquitoes. Sporozoites form within oocysts at the midgut wall of the mosquito, egress from oocysts and enter salivary glands prior to transmission. The GPI-anchored major surface protein, the circumsporozoite protein (CSP) is important for Plasmodium sporozoite formation, egress, migration and invasion. To visualize CSP, we previously generated full-length versions of CSP internally tagged with the green fluorescent protein, GFP...
February 23, 2024: Molecular Microbiology
https://read.qxmd.com/read/38394394/an-unreported-case-of-intraoperative-frozen-section-diagnosis-of-a-sclerosing-stromal-tumor-of-the-ovary-a-report-with-a-review
#28
JOURNAL ARTICLE
Pavithra Ayyanar, Mukund N Sable, Pruthwiraj Sethi, Niraj Prasad, Pritinanda Mishra
Sclerosing stromal tumor is a benign sex cord-stromal tumor, that commonly occurs in the second and third decades of age. Intraoperative diagnosis of this entity poses a great challenge because of the rare occurrence and can mimic malignant lesions. A 15-year-old female presented with a right ovarian mass. Serum markers were within normal limits. The radiological evaluation showed a large heterogeneously enhancing solid cystic abdominopelvic mass of size 16 × 14 × 9 cm with non-visualization of both ovaries separately and a few areas of calcification with mild ascites...
November 9, 2023: Indian Journal of Pathology & Microbiology
https://read.qxmd.com/read/38392906/comparing-the-fate-and-transport-of-ms2-bacteriophage-and-sodium-fluorescein-in-a-karstic-chalk-aquifer
#29
JOURNAL ARTICLE
Daniel Matthews, Simon Bottrell, Landis Jared West, Louise Maurice, Andrew Farrant, Sarah Purnell, Danny Coffey
Groundwater flow and contaminant migration tracing is a vital method of identifying and characterising pollutant source-pathway-receptor linkages in karst aquifers. Bacteriophages are an attractive alternative tracer to non-reactive fluorescent dye tracers, as high titres (>1012 pfu mL-1 ) can be safely released into the aquifer, offering improved tracer detectability. However, the interpretation of bacteriophage tracer breakthrough curves is complicated as their fate and transport are impacted by aquifer physicochemical conditions...
February 13, 2024: Pathogens
https://read.qxmd.com/read/38391307/orbital-spindle-cell-hemangioma-with-acute-presentation
#30
JOURNAL ARTICLE
Aishwarya Anand, Sridhar Shanmugam, Anand Veluswamy
We report an unusual presentation of an orbital spindle cell hemangioma in a 40-year-old male, who noted sudden redness and swelling of the left eye on waking up. On examination, the patient was found to have edema of upper eyelid edema, periorbital ecchymosis, and subconjunctival hemorrhage in the left eye at presentation. On treatment with topical medications, patient had transient symptomatic relief; however, he later developed blurring of vision. When seen 10 days later, the patient's left eye showed axial proptosis...
July 19, 2023: Indian Journal of Pathology & Microbiology
https://read.qxmd.com/read/38389158/effect-of-l-carnitine-supplementation-on-osteoarthritis-a-systematic-review
#31
REVIEW
Haiyang Kou, Bo Li, Zhili Wang, Jianbing Ma
SCOPE: Comprehensive assessment of l-carnitine's safety and effectiveness in reducing inflammatory markers in osteoarthritis (OA) patients. METHODS AND RESULTS: Journal articles on l-carnitine for OA are gathered using computer searches of PubMed, Embase, the Cochrane Library, and Web of Science. The kind of literature that is found is restricted to clinical randomized controlled trials (RCTs). The Cochrane Handbook risk of bias assessment tool RevMan 5.4 software is used to conduct a meta-analysis...
February 22, 2024: Molecular Nutrition & Food Research
https://read.qxmd.com/read/38387861/rates-of-endophthalmitis-before-and-after-transition-from-povidone-iodine-to-aqueous-chlorhexidine-asepsis-for-intravitreal-injection
#32
JOURNAL ARTICLE
Kirk Aj Stephenson, Andrew Merkur, Andrew Kirker, David Albiani, Kaivon Pakzad-Vaezi
OBJECTIVE: To assess the rate of post-intravitreal injection endophthalmitis between 2 asepsis groups: aqueous chlorhexidine 0.1% and povidone-iodine 5%. DESIGN: Retrospective, observational cohort study. PARTICIPANTS: Patients with infectious endophthalmitis post intravitreal injection (n = 58) at a single centre from July 2009 to July 2022. METHODS: Retrospective chart review of all patients receiving intravitreal injections (216 593 injections) at a single centre over 14 years...
February 19, 2024: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://read.qxmd.com/read/38380098/characterization-and-toxicological-potential-of-alternaria-alternata-associated-with-post-harvest-fruit-rot-of-prunus-avium-in-china
#33
JOURNAL ARTICLE
Tanvir Ahmad, Fuguo Xing, Changyu Cao, Yang Liu
Post-harvest fruit rot caused by Alternaria species is one of the most important threats to the fruit industry. Post-harvest rot on sweet cherry ( Prunus avium ) fruit was observed in the fruit markets of the Haidian district of Beijing, China. The fungal isolates obtained from the infected sweet cherry fruits matched the descriptions of Alternaria alternata based on the morphology and multi-gene (ITS, endo-PG, and Alta1) sequence analysis. Pathogenicity tests indicated that ACT-3 was the most virulent isolate, exhibiting typical post-harvest fruit rot symptoms...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38376829/effects-of-flooring-types-on-teat-end-bacteria-counts-milk-quality-hygiene-and-behaviour-of-dairy-cows-housed-in-tie-stall-closed-barn
#34
JOURNAL ARTICLE
Recep Aydın, Abdülkerim Diler, Veysel Fatih Ozdemir, Mete Yanar, Rıdvan Koçyiğit
This study aimed to investigate environmental mastitis causing bacteria counts in the teat end, somatic cell counts (SCC) of milk samples, cleanliness scores and behavior of cows kept on concrete and rubber mat floorings. For this purpose, 19 Holstein-Friesian dairy cows were allocated into concrete and rubber mat groups. Swab samples were taken from the teat ends to determine the bacterial counts causing environmental mastitis. Milk samples were collected from a composite of all four quarters to determine the SCC...
July 31, 2023: Veterinaria Italiana
https://read.qxmd.com/read/38367023/cell-free-supernatants-of-probiotic-consortia-impede-hyphal-formation-and-disperse-biofilms-of-vulvovaginal-candidiasis-causing-candida-in-an-ex-vivo-model
#35
JOURNAL ARTICLE
Sudaarsan Aruna Senthil Kumar, Dhesiga Krishnan, Sowndarya Jothipandiyan, Ramyadevi Durai, B Narayanan Vedha Hari, Paramasivam Nithyanand
Vulvovaginal candidiasis is the second most common vaginal infection caused by drug-resistant Candida species that affects about 70-75% of reproductive age group women across the globe. As current-day antifungal drugs are ineffective against the biofilms formed by the drug-resistant Candida strains, several natural compounds and antagonistic microbes are being explored as alternative antifungal agents. In the present study, we investigated the anti-biofilm activity of Cell-Free Supernatant (CFS) extracted from the commercially available probiotics VSL-3 against the biofilms of Candida species and also evaluated their efficacy in curbing the yeast-to-hyphal transition...
February 17, 2024: Antonie Van Leeuwenhoek
https://read.qxmd.com/read/38365249/specialized-digestive-mechanism-for-an-insect-bacterium-gut-symbiosis
#36
JOURNAL ARTICLE
Junbeom Lee, Bohyun Jeong, Jeongtae Kim, Jae H Cho, Jin H Byeon, Bok L Lee, Jiyeun K Kim
In Burkholderia-Riptortus symbiosis, the host bean bug Riptortus pedestris harbors Burkholderia symbionts in its symbiotic organ, M4 midgut, for use as a nutrient source. After occupying M4, excess Burkholderia symbionts are moved to the M4B region, wherein they are effectively digested and absorbed. Previous studies have shown that M4B has strong symbiont-specific antibacterial activity, which is not because of the expression of antimicrobial peptides but rather because of the expression of digestive enzymes, mainly cathepsin L protease...
January 8, 2024: ISME Journal
https://read.qxmd.com/read/38364098/evaluation-of-chromagar-candida-plus-for-the-detection-of-c-auris-with-a-panel-of-206-fungal-isolates-and-83-colonization-screening-skin-swabs
#37
JOURNAL ARTICLE
Meghan L Bentz, Ngoc Le, Brian Min, Natalie S Nunnally, Vickie Sullivan, Mike Tran, Shawn R Lockhart, Ana Litvintseva, Elizabeth L Berkow, D Joseph Sexton
CHROMagar Candida Plus is a new formulation of chromogenic media designed for the detection and differentiation of major clinical Candida species, including Candida auris . The objective of this study is to evaluate CHROMagar Candida Plus when used according to manufacturer's instructions with a panel of 206 fungal isolates and 83 skin-swab specimens originally collected for C. auris colonization screening. Of the 68 C . auris isolates tested, 66/68 displayed the expected light-blue colony morphology and blue halo within 48 h...
February 16, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38363936/elucidation-of-the-mechanism-of-zhenbao-pills-for-the-treatment-of-spinal-cord-injury-by-network-pharmacology-and-molecular-docking-a-review
#38
REVIEW
Mengru Xu, Wenwen Zhang, Sheng Xu, Xiaochen Niu, Li Wang, Xiaohui Wang, Haihu Hao
To explore the mechanism of the Zhenbao pill (ZBP) in treating spinal cord injury (SCI). The TCMSP Database, HERB Database and literature search were used to screen the effective ingredients and targets of ZBP; SCI-related genes were searched in GeneCards, OMIM, PharmGkb, TTD and DrugBank databases; the potential targets of ZBP for treating SCI were predicted and Venn diagrams were drawn, and the "herb-ingredient-target" network was constructed by Cytoscape software. The PPI network was constructed by STRING software, and the core targets were screened by cytoNCA plug-in; GO enrichment and KEGG pathway analysis were performed on the predicted targets using the DAVID Platform, and visualized with the Microbiology Network Platform...
February 16, 2024: Medicine (Baltimore)
https://read.qxmd.com/read/38363687/protocol-for-analysis-of-intracellular-conversion-of-artezomib-molecules-into-new-proteasome-inhibitors-in-plasmodium-falciparum-parasites
#39
JOURNAL ARTICLE
Wenhu Zhan, Yi Jing Liu, Laura A Kirkman, Gang Lin
Artezomibs (ATZs), dual-pharmacophore molecules comprising of artemisinin and a parasite proteasome inhibitor, hijack parasite ubiquitin proteasome system to transform into new proteasome inhibitors following the activation of artemisinin by heme.1 Here, we present a protocol for using a fluorescent activity-based broad-spectrum proteasome inhibitor probe to study intracellular conversion of ATZ molecules into new proteasome inhibitors in malaria parasites. We describe steps for drug treatment and washout, parasite lysis, proteasome labeling, and visualization...
February 14, 2024: STAR protocols
https://read.qxmd.com/read/38362496/use-of-a-candida-albicans-sc5314-pacbio-hifi-reads-dataset-to-close-gaps-in-the-reference-genome-assembly-reveal-a-subtelomeric-gene-family-and-produce-accurate-phased-allelic-sequences
#40
JOURNAL ARTICLE
Lois L Hoyer, Brian A Freeman, Elizabeth K Hogan, Alvaro G Hernandez
Candida albicans SC5314 is the most-often used strain for molecular manipulation of the species. The SC5314 reference genome sequence is the result of considerable effort from many scientists and has advanced research into fungal biology and pathogenesis. Although the resource is highly developed and presented in a phased diploid format, the sequence includes gaps and does not extend to the telomeres on its eight chromosome pairs. Accurate SC5314 genome assembly is complicated by the presence of extensive repeated sequences and considerable allelic length variation at some loci...
2024: Frontiers in Cellular and Infection Microbiology
keyword
keyword
88575
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.