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https://www.readbyqxmd.com/read/28631591/comparative-evaluation-of-veriflow-%C3%A2-listeria-species-to-usda-culture-based-method-for-the-detection-of-listeria-spp-in-food-and-environmental-samples
#1
Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Amrita Puri, Benjamin J Pascal, Zara E Gaudioso, Nicholas A Siciliano
Veriflow(®)Listeria species (Veriflow LS) isa molecular-based assay for the presumptive detection of Listeria spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile) and ready-to-eat (RTE) food matrixes (hot dogs and deli meat). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only a 24 h enrichment for maximum sensitivity. The Veriflow LS system eliminates the need for sample purification, gel electrophoresis, or fluorophore-based detection of target amplification and does not require complex data analysis...
June 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28631590/comparative-evaluation-of-veriflow-%C3%A2-salmonella-species-to-usda-and-fda-culture-based-methods-for-the-detection-of-salmonella-spp-in-food-and-environmental-samples
#2
Amrita Puri, Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Zara E Gaudioso, Nicholas A Siciliano
Veriflow(®)Salmonella species (Veriflow SS)is a molecular-based assay for the presumptive detection of Salmonella spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile), dairy (2% milk), raw meat (20% fat ground beef), chicken carcasses, and ready-to-eat (RTE) food (hot dogs). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only an 18 h enrichment for maximum sensitivity...
June 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28604577/pain-after-cochlear-implantation-an-unusual-complication
#3
Charlotte Celerier, Isabelle Rouillon, Marion Blanchard, Marine Parodi, Françoise Denoyelle, Natalie Loundon
OBJECTIVE: To analyze clinical signs and better define the underestimated long-term pain after implantation, to discuss etiological hypothesis, and to propose our department treatment algorithm and results. STUDY DESIGN AND SETTING: Retrospective review of children implanted with complaints of atypical pain in the area of device implantation, not in the immediate postoperative period, more than or equal to 4 on the Visual Analog Pain Scale (VAPS: 0-10) in the ENT pediatric department of Trousseau and Necker Enfants Malades Hospitals between 1998 and 2015...
June 9, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28573992/nocardia-infection-following-intraocular-surgery-report-of-seven-cases-from-a-tertiary-eye-hospital
#4
Devesh Sharma, Umang Mathur, Abha Gour, Manisha Acharya, Nidhi Gupta, Neelam Sapra
PURPOSE: To study the outcome of ocular nocardiosis following intraocular surgery. MATERIALS AND METHODS: A retrospective review of medical records of all postoperative cases of culture proven Nocardia infection over a period of 3 years, from October 2010 to September 2013, was performed. Microbiological analysis was performed for all cases and included smears and cultures. Fortified 2% amikacin eye drops were the mainstay of treatment. Surgical intervention was performed in case of nonresponse to medical therapy or suspected endophthalmitis...
May 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28561954/evidence-for-the-presence-of-a-bacterial-endosymbiont-in-the-pecan-scab-pathogen-venturia-effusa-basyonym-fusicladium-effusum
#5
E G Medrano, L J Grauke, R L Stanford, T Thompson
AIMS: To determine whether Venturia effusa the causative fungal agent of pecan scab harbors a bacterial symbiont. METHODS AND RESULTS: Venturia effusa isolates were maintained on potato dextrose agar amended with antibiotics (chloramphenicol 100 μg ml(-1) , and tetracycline 100 μg ml(-1) ). Genomic DNA extracted from mycelia was used to target eubacterial 16S rDNA. A 1(.) 4 kbp PCR amplified product using 16S rDNA degenerate primers was cloned, sequenced and found to have 99% identities with Actinobacteria representatives...
May 31, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28547176/metabolic-volume-performs-better-than-suvmax-in-the-detection-of-left-ventricular-assist-device-driveline-infection
#6
Nemanja Avramovic, Angelo Maria Dell'Aquila, Matthias Weckesser, Danka Milankovic, Alexis Vrachimis, Jürgen R Sindermann, Christian Wenning
PURPOSE: A continuous-flow left ventricular assist device (LVAD) is a new and highly promising therapy in supporting end-stage heart failure patients, either bridging them to heart transplantation or as a destination therapy. Infection is one of the major complications associated with LVAD implants. (18)F-FDG PET/CT has already been shown to be useful in the detection of LVAD infection. The goal of this study was to compare the diagnostic accuracy of different PET analysis techniques (visual grading versus SUVmax and metabolic volume)...
May 25, 2017: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28541964/role-of-early-vitrectomy-in-the-treatment-of-fungal-endophthalmitis
#7
Umesh Chandra Behera, Monica Budhwani, Taraprasad Das, Soumyava Basu, Tapas Ranjan Padhi, Manas Ranjan Barik, Savitri Sharma
PURPOSE: To study whether immediate pars plana vitrectomy at the time of sampling vitreous for culture alter the visual and structural outcomes in fungal endophthalmitis. METHODS: Surgical results of 66 consecutive patients with culture-positive fungal endophthalmitis attending a tertiary eye care hospital in eastern India were analyzed. Based on the timing of vitrectomy, they were divided into 2 groups: Group 1-immediate vitrectomy (n = 31) and Group 2-diagnostic vitrectomy alone (n = 35) as the first-line intervention...
May 23, 2017: Retina
https://www.readbyqxmd.com/read/28534320/infectious-keratitis-and-orthokeratology-lens-use-a-systematic-review
#8
REVIEW
Ka Wai Kam, Wing Yung, Gabriel Ka Hin Li, Li Jia Chen, Alvin L Young
PURPOSE: Myopia is a prevalent condition among Asians. Orthokeratology lens has gained popularity as a method of myopia control. This systematic review is to summarize the clinical profile of infectious keratitis in association with orthokeratology lens wear. METHODS: We searched in the PubMed and EMBASE for articles adopting the search strategy "(orthokeratology lens OR orthokeratology) AND (bacterial eye infection OR keratitis OR cornea ulcer OR microbial keratitis OR bacterial keratitis)", from the start date of the databases to August 23, 2016...
May 22, 2017: Infection
https://www.readbyqxmd.com/read/28524886/fungal-keratitis-in-the-republic-of-ireland
#9
S Farrell, E McElnea, S Moran, S Knowles, C C Murphy
PurposeFungal keratitis is relatively rare in temperate climates with only one previous population-based study, which reported a minimum annual incidence of 0.32 per million population in the United Kingdom. We performed a nationwide study in the Republic of Ireland of cases over a 6-year period to establish the incidence, risk factors, treatment, and outcomes of fungal keratitis.Patients and methodsAll corneal scraping specimens positive for fungal species over a 6-year period were identified at the microbiology departments of the seven main ophthalmology units in the Republic of Ireland...
May 19, 2017: Eye
https://www.readbyqxmd.com/read/28518116/the-wincf-model-an-inexpensive-and-tractable-microcosm-of-a-mucus-plugged-bronchiole-to-study-the-microbiology-of-lung-infections
#10
William J Comstock, Edwin Huh, Reiley Weekes, Connor Watson, Tianyang Xu, Pieter C Dorrestein, Robert A Quinn
Many chronic airway diseases result in mucus plugging of the airways. Lungs of an individual with cystic fibrosis are an exemplary case where their mucus-plugged bronchioles create a favorable habitat for microbial colonization. Various pathogens thrive in this environment interacting with each other and driving many of the symptoms associated with CF disease. Like any microbial community, the chemical conditions of their habitat have a significant impact on the community structure and dynamics. For example, different microorganisms thrive in differing levels of oxygen or other solute concentrations...
May 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28513490/coexistence-of-herpes-simplex-virus-infection-in-microsporidial-stromal-keratitis-associated-with-granulomatous-inflammation
#11
Ruchi Mittal, Praveen K Balne, Srikant Sahu, Sujata Das, Savitri Sharma
BACKGROUND: Microsporidial stromal keratitis poses several diagnostic challenges. Patients may present with corneal ulceration, marked stromal thinning, or even as a quite corneal scar. The presentation of microsporidial stromal keratitis commonly mimics viral keratitis. Microbiology scrapings are usually helpful; however, scraping and culture-negative cases pose a significant diagnostic dilemma. Histopathological examination is diagnostic but shows varying degree of inflammation, predominantly composed of polymorphonuclear leukocytes...
April 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28513297/former-food-products-safety-microbiological-quality-and-computer-vision-evaluation-of-packaging-remnants-contamination
#12
M Tretola, A R Di Rosa, E Tirloni, M Ottoboni, C Giromini, F Leone, C E M Bernardi, V Dell'Orto, V Chiofalo, L Pinotti
The use of alternative feed ingredients in farm animal's diets can be an interesting choice from several standpoints, including safety. In this respect, the aim of this study was to investigate the safety features of selected Former Food Products (FFPs) intended for animal nutrition produced in the framework of IZS PLV 06/14 RC project by a FFP processing plant. Six FFP samples, both mash and pelleted, were analysed for the enumeration of Total Viable Count (TVC) (ISO 4833), Enterobacteriaceae (ISO 21528-1), Escherichia coli (ISO 16649-1), coagulase-positive Staphylococci (CPS) (ISO 6888), presumptive B...
May 17, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28512715/management-of-rainwater-harvesting-and-its-impact-on-the-health-of-people-in-the-middle-east-case-study-from-yatta-town-palestine
#13
Ilke Celik, Lina M A Tamimi, Issam A Al-Khatib, Defne S Apul
Water-related diseases are a primary problem in Palestine where many residents revert to harvested rainwater as their primary water source due to water shortages within the area. From an environmental engineering perspective, it is already well known that certain situations (e.g., cross contamination) reduce drinking water quality and ultimately cause diseases in a population. In this study, we investigated the social practices and situations that may lead to lower disease occurrence. Towards this goal, we surveyed 382 residents in Yatta to collect data on the water-related diseases that they experienced and the specific situations that might affect the disease occurrences such as the residents' practices (i) for maintaining a high quality of cistern water, (ii) for maintaining the environment around the cistern, and (iii) for managing the wastewater...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28486311/free-living-amoebae-keratitis
#14
Antonio Pinna, Tiziana Porcu, Francesco Boscia, Antonella Cano, Giuseppe Erre, Antonella Mattana
PURPOSE: To describe the diagnostic and clinical features and treatment results in 43 consecutive patients with microbiologically proven free-living amoebae (FLA) keratitis. METHODS: In this hospital-based, prospective case series, corneal scrapings from 43 patients with presumed amoebic keratitis were plated on nonnutrient agar. Amoebic isolates were identified morphologically and by the polymerase chain reaction. All patients with culture-proven FLA keratitis were treated with polyhexamethylene biguanide (PHMB) 0...
July 2017: Cornea
https://www.readbyqxmd.com/read/28476771/signature-of-microbial-dysbiosis-in-periodontitis
#15
Vincent Meuric, Sandrine Le Gall-David, Emile Boyer, Luis Acuña-Amador, Bénédicte Martin, Shao Bing Fong, Frederique Barloy-Hubler, Martine Bonnaure-Mallet
Periodontitis is driven by a disproportionate host inflammatory immune response induced by an imbalance in the composition of oral bacteria. It instigates microbial dysbiosis along with failed resolution of the chronic destructive inflammation.The objective of this study is to identify microbial signatures for health and chronic periodontitis at the genus level and to propose a model of dysbiosis including the calculation of bacterial ratios. DESIGN AND METHODS: Published sequencing data obtained from several different studies (196 sub-gingival samples from patients with chronic periodontitis and 422 sub-gingival samples from healthy subjects) were pooled and submitted to a new microbiota analysis using the same VAMPS (Visualization and Analysis of Microbial Population Structures) analysis pipeline to identify microbiota specific to health and disease...
May 5, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28448296/the-use-of-the-moleculight-i-x-in-managing-burns-a-pilot-study
#16
Emily Blumenthal, Steven L A Jeffery
The MolecuLight i:X Imaging Device is a portable, noninvasive, real-time camera used to visualize the bacterial load in a wound. It uses violet light illumination and a dual bandpass optical filter to capture the fluorescence of endogenous structures in the tissue matrix and harmful bacteria. The MolecuLight i:X captures images of wounds and highlights potentially detrimental levels of bacteria. This is an initial evaluation of using the MolecuLight i:X camera in the management of burns to demonstrate the following: the ability of the device to guide clinicians in their management of the burn (ie, detect, identify, and specify swabbing locations)...
April 21, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28439137/compatibility-of-ceftazidime-avibactam-ceftolozane-tazobactam-and-piperacillin-tazobactam-with-vancomycin-in-dextrose-5-in-water
#17
Kevin Meyer, Maressa Santarossa, Larry H Danziger, Eric Wenzler
Objectives: The compatibility of vancomycin with existing and novel β-lactam/β-lactamase inhibitors at clinically relevant concentrations in 5% dextrose in water has not been fully explored to date. Methods: Vancomycin concentrations tested ranged from 5 to 20 mg/mL. Ceftazidime-avibactam was tested at 8, 20, and 40 mg/mL, ceftolozane-tazobactam at 15 mg/mL, and piperacillin-tazobactam at 28 mg/mL. Compatibility of drug admixtures were tested via both simulated and actual y-site infusion. For the simulated y-site compatibility assessment, 1:1 mixtures of each respective drug were analyzed over 24 hours...
March 2017: Hospital Pharmacy
https://www.readbyqxmd.com/read/28406861/clinical-presentation-microbiologic-profile-and-factors-predicting-outcomes-in-bacillus-endophthalmitis
#18
Vivek P Dave, Avinash Pathengay, Ina Budhiraja, Savitri Sharma, Rajeev R Pappuru, Mudit Tyagi, Raja Narayanan, Subhadra Jalali, Jay Chhablani, Taraprasad Das
PURPOSE: To describe the clinical presentation, microbiologic profile, and factors predicting outcomes in Bacillus endophthalmitis. METHODS: Retrospective interventional case series. Eighty-six patients with culture-proven Bacillus endophthalmitis, from January 2001 to December 2015, underwent vitrectomy/vitreous biopsy and intravitreal antibiotic with or without steroid as appropriate. The undiluted vitreous biopsy was subjected to microbiologic evaluation. The duration of symptoms, presenting visual acuity, organisms isolated, influence of intravitreal dexamethasone with intravitreal antibiotics, and type of initial intervention were examined for any clinical and statistical correlation in terms of odds ratio with the final visual outcome...
April 11, 2017: Retina
https://www.readbyqxmd.com/read/28404759/paving-the-way-for-dose-banding-of-chemotherapy-an-analytical-approach
#19
Heike Reinhardt, Rainer Trittler, Alison G Eggleton, Stefan Wöhrl, Thomas Epting, Marion Buck, Sabine Kaiser, Daniel Jonas, Justus Duyster, Manfred Jung, Martin J Hug, Monika Engelhardt
Background: In an interdepartmental cooperation, we investigated the feasibility and benefits of implementing dose banding of chemotherapy at our medical center. Based on this concept, chemotherapy doses are clustered into bands of similar dosage levels, thereby allowing the preproduction of frequently used standard doses of drugs, with sufficient physicochemical stability. Although established practice in the United Kingdom, there is little published evidence of its introduction elsewhere. Methods: We performed an analysis of local prescribing practice (22,310 chemotherapies) and identified gemcitabine, 5-fluorouracil, and carboplatin, among various others, as cytotoxic drugs suitable for dose banding...
April 2017: Journal of the National Comprehensive Cancer Network: JNCCN
https://www.readbyqxmd.com/read/28385473/the-utility-of-repeat-culture-in-fungal-corneal-ulcer-management-a-secondary-analysis-of-the-mutt-i-randomized-clinical-trial
#20
Kathryn J Ray, Prajna Lalitha, N Venkatesh Prajna, Revathi Rajaraman, Tiruvengada Krishnan, Muthiah Srinivasan, Peter Ryg, Stephen McLeod, Nisha R Acharya, Thomas M Lietman, Jennifer Rose-Nussbaumer
PURPOSE: To determine whether patients who had a positive repeated culture was predictive of worse clinical outcome than those who achieved microbiological cure at 6 days in the Mycotic Ulcer Treatment Trial I (MUTT-I). DESIGN: Secondary analysis from a multicenter, double-masked, randomized clinical trial. METHODS: setting: Multiple hospital sites of the Aravind Eye Care System, India. STUDY POPULATION: Patients with culture-positive filamentous fungal ulcers and visual acuity of 20/40 to 20/400 reexamined 6 days after initiation of treatment...
June 2017: American Journal of Ophthalmology
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