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visual microbiology

David T Truong, Minh-Thuy Bui, H Dwight Cavanagh
PURPOSE: To review the epidemiology, risk factors, microbiologic spectrum, and treatment of microbial keratitis during a 5-year period at an urban public hospital in comparison with an adjacent private university practice. METHODS: Retrospective chart review in the 5-year interval, 2009 through 2014. Primary outcome measures included patient age at presentation, best-corrected visual acuity (BCVA), risk factors, culture and sensitivities, treatment, and complication occurrence...
October 7, 2016: Eye & Contact Lens
Linnea K Honeker, Robert A Root, Jon Chorover, Raina M Maier
Metal(loid)-contamination of the environment due to anthropogenic activities is a global problem. Understanding the fate of contaminants requires elucidation of biotic and abiotic factors that influence metal(loid) speciation from molecular to field scales. Improved methods are needed to assess micro-scale processes, such as those occurring at biogeochemical interfaces between plant tissues, microbial cells, and metal(loid)s. Here we present an advanced method that combines fluorescence in situ hybridization (FISH) with synchrotron-based multiple-energy micro-focused X-ray fluorescence microprobe imaging (ME μXRF) to examine colocalization of bacteria and metal(loid)s on root surfaces of plants used to phytostabilize metalliferous mine tailings...
September 29, 2016: Journal of Microbiological Methods
Shreesha Kumar Kodavoor, Ramamurthy Dandapani, Ajay Ramesh Kaushik
A 32-year-old female patient underwent deep anterior lamellar keratoplasty (DALK) in both eyes for advanced keratoconus. She developed an infiltrate in the nasal paracentral interface of the left eye. In view of the paracentral location, localized epithelial removal/stromal scraping was done and the infiltrate was removed using forceps and sent for microbiological assay, which revealed fungal filaments. The patient successfully responded to intensive topical antifungal therapy with the maintenance of visual acuity...
August 2016: Indian Journal of Ophthalmology
Cindy X Zheng, Marlene R Moster, M Ali Khan, Allen Chiang, Sunir J Garg, Yang Dai, Michael Waisbourd
PURPOSE: To report the clinical features, microbial spectrum, and treatment outcomes of endophthalmitis after glaucoma drainage implant (GDI) surgery. METHODS: Records of patients diagnosed with endophthalmitis after GDI surgery were reviewed. Data on clinical course, microbiological laboratory results, and treatment were analyzed. RESULTS: Of 1,891 eyes that underwent GDI surgery, 14 eyes (0.7%) developed endophthalmitis. The mean time interval between GDI surgery and diagnosis of endophthalmitis was 2...
September 26, 2016: Retina
M J Mendell, K Kumagai
An important proportion of respiratory illness is considered attributable to residential dampness or mold (D/M). Developing health-protective D/M guidelines has been challenging, in part because unhealthy levels of indoor D/M cannot be defined using available microbiological measurements. This review paper explores reported multilevel, observation-based (eg visual or olfactory) D/M metrics for potential in defining unhealthy levels of residential D/M. For many of the 33 multilevel residential D/M metrics identified, health risks generally increased as observed D/M increased...
September 24, 2016: Indoor Air
Ella H Leung, Ajay E Kuriyan, Harry W Flynn, Nidhi Relhan, Laura C Huang, Darlene Miller
PURPOSE: To report the clinical settings, microbiological isolates, and best corrected visual acuities (BCVA) of patients with persistently culture-positive exogenous fungal endophthlamitis. DESIGN: Retrospective, consecutive, case series. METHODS: Setting: Tertiary referral center. PATIENT POPULATION: 16 eyes of 16 patients with at least two consecutive positive vitreous cultures between 1981-2015. INTERVENTIONS: Intravitreal antifungal injections, pars plana vitrectomy (PPV) Main Outcome Measure: Clinical settings, microbiologic isolates, BCVA RESULTS: The most common clinical settings were after cataract surgery (9/16, 56%), glaucoma surgery (4/16, 25%), and trauma (2/16, 13%)...
September 16, 2016: American Journal of Ophthalmology
Chongcong Wu, Sravya Nakka, Sepahdar Mansouri, Torbjörn Bengtsson, Tayeb Nayeri, Fariba Nayeri
BACKGROUND: There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host's defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth of other microorganisms. Current antimicrobial strategies often fail due to poor diagnosis of key pathogens in biofilms. The study aims to develop anti-bacterial human antibodies in vitro from patients who had recently undergone a systemic infection by pathogenic bacteria and to use these antibodies as a tool for detecting bacteria in biofilms...
2016: BMC Microbiology
Waleed M Sweileh, Sa'ed H Zyoud, Samah W Al-Jabi, Ansam F Sawalha, Naser Y Shraim
BACKGROUND: Water - related diseases are worldwide health concern. Microbial contamination and contaminant products in water are a source of disease outbreaks and development of cumulative toxic effects. Ensuring safe water is one of the goals to be achieved at the global level. The aim of this study was to assess publications on drinking and recreational water from a health point of view to understand current problems and future research trends in this field. METHODS: Scopus, the largest scientific electronic database, was used to retrieve related articles and present the results as bibliometric tables and maps...
2016: Annals of Occupational and Environmental Medicine
Giorgio Lombardo, Antonio D'Agostino, Lorenzo Trevisiol, Maria Grazia Romanelli, Anna Mascellaro, Macarena Gomez-Lira, Alessia Pardo, Vittorio Favero, Pier Francesco Nocini
OBJECTIVES: To assess the clinical, microbiologic, and radiologic status of soft and hard tissues surrounding zygomatic implants. STUDY DESIGN: Patients who had at least two zygomatic implants were eligible for the study. Their soft tissues were analyzed, and microbial samples were collected. Cone beam computed tomography (CBCT) and orthopantomography were used to measure bone levels. The patients were also asked to complete a Visual Analogue Scale (VAS) questionnaire assessing their satisfaction...
November 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Jennifer L Guthrie, David C Alexander, Alex Marchand-Austin, Karen Lam, Michael Whelan, Brenda Lee, Colin Furness, Elizabeth Rea, Rebecca Stuart, Julia Lechner, Monali Varia, Jennifer McLean, Frances B Jamieson
OBJECTIVE: Develop a tool to disseminate integrated laboratory, clinical, and demographic case data necessary for improved contact tracing and outbreak detection of tuberculosis (TB). METHODS: In 2007, the Public Health Ontario Laboratories implemented a universal genotyping program to monitor the spread of TB strains within Ontario. Ontario Universal Typing of TB (OUT-TB) Web utilizes geographic information system (GIS) technology with a relational database platform, allowing TB control staff to visualize genotyping matches and microbiological data within the context of relevant epidemiological and demographic data...
September 2, 2016: Journal of the American Medical Informatics Association: JAMIA
I-Huang Lin, Yi-Sheng Chang, Sung-Huei Tseng, Yi-Hsun Huang
Infectious keratitis after penetrating keratoplasty (PK) is a devastating condition that may result in graft failure and poor visual outcome. In this study, we retrospectively reviewed the medical records of patients who underwent PK between 2009 and 2014, and recorded those who developed infectious keratitis. We compared the predisposing factors and organisms isolated to those identified in our previous study, conducted between 1989 and 1994. The incidence of post-PK infectious keratitis decreased from 11...
September 2, 2016: Scientific Reports
Joël Schlatter, Elodie Bourguignon, Elyes Majoul, Sofiane Kabiche, Issa-Bella Balde, Salvatore Cisternino, Jean-Eudes Fontan
Adapted forms for administration to infants are limited. The proposed study was performed to propose oral liquid formulations of idebenone in Ora-Plus and either Ora-Sweet or Ora-Sweet SF, Ora-Blend, Ora-Blend SF, and Inorpha. Each formulation was stored in 30-ml amber glass bottle at 5 or 25°C for 90 days. Idebenone content in these suspensions, determined by a stability-indicating high-performance liquid chromatography method, remained stable at least 90 days in Inorpha when stored at the two temperatures...
September 1, 2016: Pharmaceutical Development and Technology
Miguel Gil, Pedro Pinto, Alexandra S Simões, Pedro Póvoa, Miguel Mira Da Silva, Luís Velez Lapão
About 37 thousand people die per year in Europe due to infections by resistant bacteria. Fighting antimicrobial resistances (AR) is a top priority to save lives and reduce costs. AR is triggered mostly by uncritical antibiotic prescription. This paper presents HAITool, a decision-making information system to support antibiotic prescription. The system was co-developed together with health professionals using Design Science Research Methodology, empowered with innovative data visualization techniques to improve AR management...
2016: Studies in Health Technology and Informatics
Oleci P Frota, Adriano M Ferreira, Rodrigo Koch, Denise de Andrade, Marcelo A Rigotti, Najla M A Borges, Margarete T G Almeida
BACKGROUND: Cleaning of surfaces is essential in reducing environmental bioburdens and health care-associated infection in emergency units. However, there are few or no studies investigating cleaning surfaces in these scenarios. Our goal was to determine the influence of a multifaceted intervention on the effectiveness of routine cleaning of surfaces in a walk-in emergency care unit. METHODS: This prospective, before-and-after interventional study was conducted in 4 phases: phase I (situational diagnosis), phase II (implementation of interventions-feedback on results, standardization of cleaning procedures, and training of nursing staff), phase III (determination of the immediate influence of interventions), and phase IV (determination of the late influence of interventions)...
August 24, 2016: American Journal of Infection Control
Capucine Odouard, Daini Ong, Parth R Shah, Thomas Gin, Penelope J Allen, John Downie, Lyndell L Lim, Peter McCluskey
BACKGROUND: Endogenous Klebsiella pneumoniae endophthalmitis (EKPE) is a well-known entity in South-East Asia. We demonstrate a range of differing clinical features and outcomes of EKPE, and highlight the increasing incidence of EKPE in major centres in Sydney and Melbourne, Australia. DESIGN: Retrospective observational case study and case series in the hospital setting. PARTICIPANTS: Four cases of EKPE. METHODS: EKPE cases from 2005 to 2015 were identified through established endophthalmitis databases as well as hospital-based microbiological searches...
August 26, 2016: Clinical & Experimental Ophthalmology
X Flores, M Vicente, S Haddad, C Amat, L Carrera, P S Corona
BACKGROUND: Bone loss in the distal femur is a common problem in knee revision surgeries. The problem is exacerbated in the context of an active infection. In extreme cases this bone loss could compromise the feasibility of a two-stage exchange protocol using dynamic spacers due to the inherent instability of this type of spacers. Use of a hip prefabricated spacer in a "reverse" way forming a ball-and- socket joint is a therapeutic option in cases of massive bone defect and infection...
August 18, 2016: Revista Española de Cirugía Ortopédica y Traumatología
Carla Sofia Ferreira, Luis Figueira, Nuno Moreira-Gonçalves, Raúl Moreira, Luis Torrão, Fernando Falcão-Reis
OBJECTIVES: To study the microbial profile, antibiotic susceptibility pattern, risk factors, therapeutic trends, and clinical outcomes for microbial keratitis (MK) in a tertiary health care center. METHODS: All cases with suspected bacterial keratitis that were followed at consultation from September 2007 to August 2015 were included. Microbial cultures were obtained and patients were managed following an internal protocol. RESULTS: Two hundred thirty-five patients were included, with a mean age of 50...
July 12, 2016: Eye & Contact Lens
Joanne Wong, Adam Sutherland
AIM: Ketamine is used for post-operative analgesia. There has been recent disruption in it's supply. It is usually prescribed by patient's weight (3 mg/kg in 50 ml 0.9% saline) at a rate of 1-5 ml/hr (1-5 microgram/kg/minute). To conserve ketamine supplies our policy was changed to a concentrated "standardised" concentration of ketamine (250 mg in 50 ml 0.9% sodium chloride) that could be run for a maximum of 72 hrs. There is evidence demonstrating no relationship between duration of infusion and microbiological contamination for 72 hrs...
September 2016: Archives of Disease in Childhood
Rickard Hammarén, Chandan Pal, Johan Bengtsson-Palme
: With decreasing costs of generating DNA sequence data, genome and metagenome projects have become accessible to a wider scientific community. However, to extract meaningful information and visualize the data remain challenging. We here introduce FARAO, a highly scalable software for organization, visualization and integration of annotation and read coverage data that can also combine output data from several bioinformatics tools. The capabilities of FARAO can greatly aid analyses of genomic and metagenomic datasets...
August 4, 2016: Bioinformatics
Eric S Ahn, Matthew J Hauck, Jonathan Kirk Harris, Charles E Robertson, Roger A Dailey
PURPOSE: To investigate the presence and microbiology of bacterial biofilms on Jones tubes (JTs) by direct visualization with scanning electron microscopy and polymerase chain reaction (PCR) of representative JTs, and to correlate these findings with inflammation and/or infection related to the JT. METHODS: In this study, prospective case series were performed. JTs were recovered from consecutive patients presenting to clinic for routine cleaning or recurrent irritation/infection...
August 1, 2016: Ophthalmic Plastic and Reconstructive Surgery
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