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https://www.readbyqxmd.com/read/29209953/diagnostic-value-of-99mtc-ethambutol-scintigraphy-in-tuberculosis-compared-to-microbiological-and-histopathological-tests
#1
A H S Kartamihardja, Y Kurniawati, R Gunawan
OBJECTIVE: Tuberculosis (TB) still remains the world's endemic infection. TB affects the lungs and any part of the body other than the lung. The diagnosis of TB has not changed much over the decades. Ethambutol is one of the first line treatments for TB. It can be labeled using 99mTc. 99mTc-ethambutol will be accumulated in the site of TB lesion and can be imaged using gamma camera. The aim of this study was to evaluate the diagnostic value of 99mTc-ethambutol scintigraphy in detecting and localizing of TB...
December 5, 2017: Annals of Nuclear Medicine
https://www.readbyqxmd.com/read/29190624/extreme-surgical-maneuvers-in-fungal-endophthalmitis
#2
María Isabel Relimpio-López, María Gessa-Sorroche, Antonio Manuel Garrido-Hermosilla, Concepción Díaz-Ruiz, Jesús Montero-Iruzubieta, Jaime Etxebarría-Ecenarro, Diego Ruiz-Casas, Enrique Rodríguez-de-la-Rúa-Franch
PURPOSE: To present the different evolution of 2 cases of endophthalmitis caused by Fusarium solani, an aggressive filamentous fungus, depending on the medical and surgical treatment performed. METHODS: We present 2 cases of endophthalmitis caused by Fusarium solani. Topical, intrastromal, intravitreal, and systemic antifungal treatment (natamycin, voriconazole, amphotericin B) failed in both cases. Corneal perforation took place in one of them, being unsuccessfully treated with cyanoacrylate and several amniotic membrane transplants...
November 30, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/29160477/efficiency-of-cleaning-and-disinfection-of-surfaces-correlation-between-assessment-methods
#3
Oleci Pereira Frota, Adriano Menis Ferreira, Odanir Garcia Guerra, Marcelo Alessandro Rigotti, Denise de Andrade, Najla Moreira Amaral Borges, Margarete Teresa Gottardo de Almeida
OBJECTIVE: to assess the correlation among the ATP-bioluminescence assay, visual inspection and microbiological culture in monitoring the efficiency of cleaning and disinfection (C&D) of high-touch clinical surfaces (HTCS) in a walk-in emergency care unit. METHOD: a prospective and comparative study was carried out from March to June 2015, in which five HTCS were sampled before and after C&D by means of the three methods. The HTCS were considered dirty when dust, waste, humidity and stains were detected in visual inspection; when ≥2...
November 2017: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/29149278/interdisciplinary-stem-education-reform-dishing-out-art-in-a-microbiology-laboratory
#4
Sarah J Adkins, Rachel K Rock, J Jeffrey Morris
In the modern educational framework, life science and visual art are usually presented as mutually exclusive subjects. Despite this perceived disciplinary contrast, visual art has the ability to engage and provoke students in ways that can have important downstream effects on scientific discovery, especially when applied in a practical setting such as a laboratory course. This review broadly examines the benefit of interdisciplinary fusions of science and art as well as recent ways in which art strategies have been used in undergraduate biology classrooms...
November 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29131507/the-disruptive-effect-of-lysozyme-on-the-bacterial-cell-wall-explored-by-an-in-silico-structural-outlook
#5
Emiliano D Primo, Lisandro H Otero, Francisco Ruiz, Sebastián Klinke, Walter Giordano
The bacterial cell wall, a structural unit of peptidoglycan polymer comprised of glycan strands consisting of a repeating disaccharide motif [N-acetylglucosamine (NAG) and N-acetylmuramylpentapeptide (NAM pentapeptide)], encases bacteria and provides structural integrity and protection. Lysozymes are enzymes that break down the bacterial cell wall and disrupt the bacterial life cycle by cleaving the linkage between the NAG and NAM carbohydrates. Lab exercises focused on the effects of lysozyme on the bacterial cell wall are frequently incorporated in biochemistry classes designed for undergraduate students in diverse fields as biology, microbiology, chemistry, agronomy, medicine, and veterinary medicine...
November 13, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29128932/atomic-force-microscopy-a-promising-tool-for-deciphering-the-pathogenic-mechanisms-of-fungi-in-cystic-fibrosis
#6
Stéphane Cuenot, Jean-Philippe Bouchara
During the past decades, atomic force microscopy (AFM) has emerged as a powerful tool in microbiology. Although most of the works concerned bacteria, AFM also permitted major breakthroughs in the understanding of physiology and pathogenic mechanisms of some fungal species associated with cystic fibrosis. Complementary to electron microscopies, AFM offers unprecedented insights to visualize the cell wall architecture and components through three-dimensional imaging with nanometer resolution and to follow their dynamic changes during cell growth and division or following the exposure to drugs and chemicals...
November 11, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29127252/validation-of-an-ergonomic-method-to-withdraw-99m-tc-radiopharmaceuticals
#7
Sandy Blondeel-Gomes, Solène Marie, Julien Fouque, Sabrina Loyeau, Olivier Madar, François Lokiec
The main objective of the present work was to ensure quality of radiopharmaceuticals syringes withdrawn with a "Spinal needle/obturator In-Stopper" system. Methods: Visual examinations and physicochemical tests are performed at T0 and T+4h for [(99m)Tc]albumin nanocolloid and T+7h for [(99m)Tc]eluate, [(99m)Tc] HydroxyMethylene DiPhosphonate and [(99m)Tc]Human Serum Albumin. Microbiological validation was performed according to European pharmacopoeia. Fingertip radiation exposure was evaluated to confirm the safety of the system...
November 10, 2017: Journal of Nuclear Medicine Technology
https://www.readbyqxmd.com/read/29111996/microbial-keratitis-after-accelerated-corneal-collagen-cross-linking-in-keratoconus
#8
Prafulla K Maharana, Pranita Sahay, M Sujeeth, Deepali Singhal, Anubha Rathi, Jeewan S Titiyal, Namrata Sharma
PURPOSE: To assess the pattern of microbial keratitis after accelerated corneal collagen cross-linkage (aCXL) in patients with keratoconus. METHOD: The medical records of cases of keratoconus that underwent aCXL from June 2014 to May 2017 were reviewed. Cases that developed microbial keratitis after aCXL were included in the study. The clinical, microbiological profile and the treatment outcomes were evaluated. RESULTS: Of 532 eyes that underwent aCXL, 7 cases developed microbial keratitis during the study period...
November 2, 2017: Cornea
https://www.readbyqxmd.com/read/29065223/influence-of-polysaccharide-based-edible-coatings-as-carriers-of-prebiotic-fibers-on-quality-attributes-of-ready-to-eat-fresh-blueberries
#9
María V Alvarez, Alejandra G Ponce, María R Moreira
BACKGROUND: Little information is available regarding the effect of dietary fibers added into edible coatings on quality attributes of ready-to-eat fruits. The aim of this study was to evaluate the effects of sodium alginate (AL) and chitosan (CH) edible coatings enriched with four different dietary fibers (apple fiber, orange fiber, inulin and oligofructose) on microbiological, nutritional, physicochemical and sensorial properties of ready-to-eat fresh blueberries stored for 18 days at 5 °C...
October 24, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29040115/keratitis-after-post-kala-azar-dermal-leishmaniasis
#10
Aditya Pradhan, Samar Basak, Tuhin Chowdhury, Ayan Mohanta, Ananya Chatterjee
PURPOSE: To report 2 cases of keratitis occurring after post-kala-azar dermal leishmaniasis (PKDL) in immunocompetent patients. METHODS: Case report and literature review. RESULTS: Two male patients presented with a dense stromal infiltrate in the temporal and superior aspects of the cornea, respectively. Microbiological workup in both patients did not show any offending organism. Both patients were undergoing treatment for PKDL in the form of oral miltefosine...
October 13, 2017: Cornea
https://www.readbyqxmd.com/read/29036747/prevention-of-multidrug-resistant-infections-from-contaminated-duodenoscopes-position-statement-of-the-european-society-of-gastrointestinal-endoscopy-esge-and-european-society-of-gastroenterology-nurses-and-associates-esgena
#11
Ulrike Beilenhoff, Holger Biering, Reinhard Blum, Jadranka Brljak, Monica Cimbro, Jean-Marc Dumonceau, Cesare Hassan, Michael Jung, Christiane Neumann, Michael Pietsch, Lionel Pineau, Thierry Ponchon, Stanislav Rejchrt, Jean-François Rey, Verona Schmidt, Jayne Tillett, Jeanin van Hooft
Patients should be informed about the benefits and risks of endoscopic retrograde cholangiopancreatography (ERCP)Only specially trained and competent personnel should carry out endoscope reprocessing.Manufacturers of duodenoscopes should provide detailed instructions on how to use and reprocess their equipment.In the case of modifications to their equipment, manufacturers should provide updated instructions for use.Detailed reprocessing protocols based on the manufacturer's instructions for use should clearly lay out the different reprocessing steps necessary for each endoscope model...
November 2017: Endoscopy
https://www.readbyqxmd.com/read/29027017/palladium-bionanoparticles-production-from-acidic-pd-ii-solutions-and-spent-catalyst-leachate-using-acidophilic-fe-iii-reducing-bacteria
#12
Naoko Okibe, Daisuke Nakayama, Takahiro Matsumoto
The acidophilic, Fe(III)-reducing heterotrophic bacteria Acidocella aromatica PFBC(T) and Acidiphilium cryptum SJH were utilized to produce palladium (Pd) bionanoparticles via a simple 1-step microbiological reaction. Monosaccharide (or intracellular NADH)-dependent reactions lead to visualization of intra/extra-cellular enzymatic Pd(0) nucleation. Formic acid-dependent reactions proceeded via the first slow Pd(0) nucleation phase and the following autocatalytic Pd(II) reduction phase regardless of the presence or viability of the cells...
November 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29016425/diagnostic-value-of-99mtc-ubiquicidin-scintigraphy-in-differentiation-between-osteomyelitis-and-bone-tumors
#13
Narjess Ayati, Mohammad Norouzi, Ramin Sadeghi, Mostafa Erfani, Mohammad Gharedaghi, Kamran Aryana
AIM: The differentiation of osteomyelitis from bone tumors is of great importance in clinical decision-making; however, the features of both osteomyelitis and bone tumors are noncontributory. Tc-ubiquicidin scintigraphy is a new promising method with the ability to specifically localize the infection site by bacterial cell membrane binding. This study aimed to evaluate the ability of this radiopeptide for the differentiation of these two entities. PATIENTS AND METHODS: Thirty consecutive patients (mean age=20...
November 2017: Nuclear Medicine Communications
https://www.readbyqxmd.com/read/28993066/clinical-and-microbiological-profile-of-infectious-keratitis-in-an-area-of-madrid-spain
#14
Soraya Mediero, Ana Boto de Los Bueis, Karina Spiess, Mariana Díaz-Almirón, Almudena Del Hierro Zarzuelo, Isabel Villalaín Rodes, Adela García Perea
INTRODUCTION: To study antibiotic susceptibility in bacterial keratitis (BK), its profile over 10 years and its influence on ophthalmological practice. METHODS: Retrospective review of BK with positive corneal scraping over a 10-year period. Risk factors for keratitis, visual acuity (VA), empirical topical treatment, corneal infection characteristics and outcomes were analyzed for BK due to Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa and Propionibacterium acnes...
October 6, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28992262/a-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-reference-database-for-the-identification-of-histoplasma-capsulatum
#15
Clara Valero, María J Buitrago, Sara Gago, Inmaculada Quiles-Melero, Julio García-Rodríguez
The isolation of the pathogenic fungus Histoplasma capsulatum from cultures together with the visualization of typical intracellular yeast in tissues are the gold standard methods for diagnosis of histoplasmosis. However, cultures are time-consuming, require level 3 containment and experienced personnel, and usually call for an additional confirmation test. Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-ToF MS) has been established as a suitable tool for microbial identification in several clinical laboratories...
July 28, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28977417/fluorine-18-fluorodeoxyglucose-positron-emission-tomography-computed-tomography-for-improving-diagnosis-of-infection-in-patients-on-cf-lvad-longing-for-more-insights
#16
Angelo M Dell'Aquila, Nemanja Avramovic, Stefano Mastrobuoni, Arash Motekallemi, Konrad Wisniewski, Mirela Scherer, Jürgen R Sindermann, Christian Wenning
Aim: Presence and consequent extent of infection in patients on continuous-flow left ventricular assist devices (CF-LVADs) can be challenging with the current diagnostic tools. The present study sought to demonstrate the diagnostic power of 18F-Fluorodeoxyglucose-Positron-Emission Tomography/Computed Tomography (18F-FDG PET/CT) in detecting infection in patients supported with CF-LVAD. Background: The present study sought to demonstrate the diagnostic power of 18F-fluorodeoxyglucose-positron-emission tomography/computed tomography (18F-FDG PET/CT) in detecting infection in patients supported with CF-LVAD...
July 12, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28971164/endophthalmitis-caused-by-corynebacterium-species-clinical-features-antibiotic-susceptibility-and-treatment-outcomes
#17
Ajay E Kuriyan, Jayanth Sridhar, Harry W Flynn, Laura C Huang, Nicolas A Yannuzzi, William E Smiddy, Janet L Davis, Thomas A Albini, Audina M Berrocal, Darlene Miller
PURPOSE: To report the clinical features, antibiotic susceptibility profiles, treatment, and visual acuity (VA) outcomes of endophthalmitis caused by Corynebacterium species. DESIGN: Retrospective case series. SUBJECTS: Patients with endophthalmitis caused by Corynebacterium species. METHODS: Microbiology database records were retrospectively reviewed for all patients with endophthalmitis caused by Corynebacterium species from January 1, 1990 to December 31, 2012 at a large university referral center...
May 2017: Ophthalmology Retina
https://www.readbyqxmd.com/read/28969572/quinoline-fluorescent-probes-for-zinc-from-diagnostic-to-therapeutic-molecules-in-treating-neurodegenerative-diseases
#18
Barbara Czaplińsk, Ewelina Spaczyńska, Robert Musioł
Background Fluorescent compounds had gained strong attention due to their wide and appealing applications. Microscopic techniques and visualization are good examples among others. Introduction of fluorescent dyes into microbiology opens the possibility to observe tissues, organisms or organelle with exceptional sensitivity and resolution. Probes for detection of biologically relevant metals as zinc, iron or copper seems to be particularly important for drug design and pharmaceutical sciences. Objective Quinoline derivatives are well known for their good metal affinity and wide spectrum of biological activity...
October 2, 2017: Medicinal Chemistry
https://www.readbyqxmd.com/read/28949429/predictors-of-visual-outcome-and-the-role-of-early-vitrectomy-in-streptococcal-endophthalmitis
#19
Emil D Kurniawan, John R Rocke, Sukhpal S Sandhu, Penelope J Allen
IMPORTANCE: Streptococcal endophthalmitis has devastating sequelae. This study aims to identify factors which may be targeted to optimize patient outcomes. BACKGROUND: This study investigated characteristics influencing visual outcomes and the role of early vitrectomy. DESIGN: Retrospective observational case series of consecutive patients was conducted. PARTICIPANTS: All patients with a culture-positive diagnosis of streptococcal endophthalmitis treated at a tertiary ophthalmology referral centre between July 1997 and February 2012 were included...
September 26, 2017: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/28900359/effectiveness-of-atp-bioluminescence-to-assess-hospital-cleaning-a-review
#20
REVIEW
N Nante, E Ceriale, G Messina, D Lenzi, P Manzi
INTRODUCTION: Contamination of hospital surfaces plays an important role in the transmission of several healthcare-associated microorganisms, therefore methods for evaluating hospital surfaces' cleaning gain particular importance. Among these, there are visual inspection, quantitative microbiology, fluorescent markers and adenosine triphosphate (ATP) bioluminescence. The latter seems to provide interesting features, detecting the presence of ATP on surface (as Relative Light Units, RLU), a proxy of organic matter and microbial contamination...
June 2017: Journal of Preventive Medicine and Hygiene
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