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visual microbiology

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https://www.readbyqxmd.com/read/29666286/viable-but-nonculturable-listeria-monocytogenes-and-salmonella-enterica-serovar-thompson-induced-by-chlorine-stress-remain-infectious
#1
Callum J Highmore, Jennifer C Warner, Steve D Rothwell, Sandra A Wilks, C William Keevil
The microbiological safety of fresh produce is monitored almost exclusively by culture-based detection methods. However, bacterial food-borne pathogens are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses such as chlorine, which is commonly used for fresh produce decontamination. Here, complete VBNC induction of green fluorescent protein-tagged Listeria monocytogenes and Salmonella enterica serovar Thompson was achieved by exposure to 12 and 3 ppm chlorine, respectively...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29656089/microbiology-catches-the-cryo-em-bug
#2
REVIEW
Lesley A Earl, Veronica Falconieri, Sriram Subramaniam
Over the past few years, the advances in technology and methods that have revolutionized cryo-EM are allowing for key insights in a variety of areas in biology, and microbiology is no exception. A wide range of important macromolecular assemblies in prokaryotic and eukaryotic cells, as well as intact viruses, have now become accessible to investigation by new methods in 3D electron microscopy. We focus here on selected examples that illustrate this breadth, and review the application of methods in single particle cryo-EM and cryo-electron tomography to progress in the structural biology of CRISPR systems, visualization of small molecule drugs in membrane proteins, in situ visualization of bacterial nanomachines, and the analysis of antigen-antibody interactions to drive vaccine design...
April 12, 2018: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29625693/-implementation-and-assessment-of-anticancer-drugs-batch-preparation-circuit-in-a-teaching-hospital
#3
M Moine, L Hachon, D Sankhare, A Ade, I Madelaine, N Jourdan
INTRODUCTION: In 2011, the anticancer preparation unit in a teaching hospital implemented a circuit for batch preparation of anticancer drugs. In this circuit, a manual and a semi-automatic production process coexist. The aim of the study is to describe the circuit in 2016 and to assess the impact of batch preparation in a anticancer preparation unit. MATERIAL AND METHODS: Eligible anticancer drugs for batch preparation satisfy two conditions: solution stability up to 28 days, prescription frequency up to one every two days...
April 3, 2018: Annales Pharmaceutiques Françaises
https://www.readbyqxmd.com/read/29595947/unique-fluorescent-probe-molecule-for-bacterial-surface-localization-and-resistant-enzyme-imaging
#4
Hui Ling Chan, Linna Lyu, Junxin Aw, Wenmin Zhang, Juan Li, Huang-Hao Yang, Hirohito Hayashi, Shunsuke Chiba, Bengang Xing
Emergence of antibiotic bacterial resistance has caused serious clinical issues worldwide due to increasingly difficult treatment. Development of a specific approach for selective visualization of resistant bacteria will be highly significant for clinical investigations to promote timely diagnosis and treatment of bacterial infections. In this article, we present an effective method that not only is able to selectively recognize drug resistant AmpC β-lactamases enzyme, but more importantly, is able to interact with bacterial cell wall components resulting in a desired localization effect on the bacterial surface...
March 29, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29593685/the-use-of-a-chimeric-rhodopsin-vector-for-the-detection-of-new-proteorhodopsins-based-on-color
#5
Alina Pushkarev, Gur Hevroni, Sheila Roitman, Jin-Gon Shim, Ahreum Choi, Kwang-Hwan Jung, Oded Béjà
Student microbial ecology laboratory courses are often conducted as condensed courses in which theory and wet lab work are combined in a very intensive short time period. In last decades, the study of marine microbial ecology is increasingly reliant on molecular-based methods, and as a result many of the research projects conducted in such courses require sequencing that is often not available on site and may take more time than a typical course allows. In this work, we describe a protocol combining molecular and functional methods for analyzing proteorhodopsins (PRs), with visible results in only 4-5 days that do not rely on sequencing...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29580152/epidemiology-risk-factors-and-clinical-outcomes-in-severe-microbial-keratitis-in-south-india
#6
Jaya Devi Chidambaram, Namperumalsamy Venkatesh Prajna, Palepu Srikanthi, Shruti Lanjewar, Manisha Shah, Shanmugam Elakkiya, Prajna Lalitha, Matthew J Burton
PURPOSE: Here, we report risk factors associated with outcome in severe bacterial keratitis (BK), fungal keratitis (FK), and Acanthamoeba keratitis (AK) in India. METHODS: Prospective observational cohort study conducted in Aravind Eye Hospital, India. Adults presenting with severe microbial keratitis (MK) were enrolled (size ≥3 mm) and followed to 21 days post-enrolment. Ulcer clinical features were recorded at presentation. Outcomes by final visit were classified as good (completely healed or reduced infiltrate size) or poor (enlarged infiltrate size, perforated, or surgery performed)...
March 26, 2018: Ophthalmic Epidemiology
https://www.readbyqxmd.com/read/29526216/shelf-life-evaluation-of-fresh-cut-red-chicory-subjected-to-different-minimal-processes
#7
Antonio Alfonzo, Raimondo Gaglio, Alessandro Miceli, Nicola Francesca, Rosalia Di Gerlando, Giancarlo Moschetti, Luca Settanni
Microbiological, chemical and physical parameters of minimally processed red chicory (Cichorium intybus L.) subjected to two different transformation processes were investigated. A classic ready-to-eat (RTE) process (P1) and a production without cutting (P2) were monitored during refrigerated (4 °C) storage (15 d). Total mesophilic microorganisms, total psychrotrophic microorganisms and pseudomonads were detected at the highest cell densities in all samples. Presumptive Pseudomonas population dominated the cultivable microbial community of RTE red chicory and were characterized genetically...
August 2018: Food Microbiology
https://www.readbyqxmd.com/read/29474356/management-of-severe-strongyloidiasis-attended-at-reference-centers-in-spain
#8
Angela Martinez-Perez, Silvia Roure Díez, Moncef Belhassen-Garcia, Diego Torrús-Tendero, Jose Luis Perez-Arellano, Teresa Cabezas, Cristina Soler, Marta Díaz-Menéndez, Miriam Navarro, Begoña Treviño, Fernando Salvador
INTRODUCTION: Strongyloides stercoralis is a globally distributed nematode that causes diverse clinical symptoms in humans. Spain, once considered an endemic country, has experienced a recent increase in imported cases. The introduction of serology helps diagnosis and is currently replacing microbiological techniques in some settings, but its sensitivity is variable and can be low in immunocompromised patients. Diagnosis can only be confirmed by identification of larvae. Often, this "gold standard" can only be achieved in severe cases, such as disseminated S...
February 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29472513/stability-of-an-extemporaneously-compounded-minoxidil-oral-suspension
#9
Yunmei Song, Zen Whey Chin, David Ellis, Ei Mon Phyo Lwin, Sean Turner, Desmond Williams, Sanjay Garg
PURPOSE: Results of a study to determine the stability of an extemporaneously compounded minoxidil oral suspension under various temperature and stress conditions are reported. METHODS: Commercially available minoxidil tablets (10 mg) were crushed to a fine powder, and predetermined amounts of 2 suspending vehicles were added to produce a 1-mg/mL suspension, which was stored in glass bottles at room temperature (25 ± 2 °C) or in a refrigerator (4 ± 2 °C). To simulate daily patient use, 5 days weekly 1 bottle of the suspension was removed from refrigerated storage and shaken and 0...
March 1, 2018: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/29468213/acute-onset-endophthalmitis-caused-by-staphylococcus-lugdunensis
#10
Robert B Garoon, Darlene Miller, Harry W Flynn
Purpose: To report a series of patients with acute-onset bacterial endophthalmitis caused by culture proven Staphylococcus lugdunensis , and to characterize clinical outcomes and microbiologic susceptibilities of this organism. Observations: The study included six eyes of 6 patients. The etiologies included cataract surgery (3), open globe injury (2), and intravitreal injection (1). Isolates of S. lugdunensis demonstrated sensitivity to vancomycin in all cases reported...
March 2018: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29426368/oxford-screening-csf-and-respiratory-samples-oscar-results-of-a-pilot-study-to-screen-clinical-samples-from-a-diagnostic-microbiology-laboratory-for-viruses-using-illumina-next-generation-sequencing
#11
Colin Sharp, Tanya Golubchik, William F Gregory, Anna L McNaughton, Nicholas Gow, Mathyruban Selvaratnam, Alina Mirea, Dona Foster, Monique Andersson, Paul Klenerman, Katie Jeffery, Philippa C Matthews
OBJECTIVES: There is increasing interest in the use of metagenomic (next generation sequencing, NGS) approaches for diagnosis of infection. We undertook a pilot study to screen samples submitted to a diagnostic microbiology laboratory in a UK teaching hospital using Illumina HiSeq. In the short-term, this small dataset provides insights into the virome of human respiratory and cerebrospinal fluid (CSF) samples. In the longer term, assimilating metagenomic data sets of this nature can inform optimization of laboratory and bioinformatic methods, and develop foundations for the interpretation of results in a clinical context...
February 9, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29426303/a-rare-case-of-scleral-buckle-infection-with-curvularia-species
#12
Shalini Singh, Ankita Shrivastav, Manisha Agarwal, Arpan Gandhi, Rahul Mayor, Lagan Paul
BACKGROUND: Scleral buckling is an established modality of treating retinal detachment. Being an external implant the buckle may be prone to infections. We report such a case with a delayed presentation and a rare etiology. CASE PRESENTATION: A 45 year old male presented with redness, foreign body sensation and discharge for one month in his right eye. The patient had undergone a retinal detachment surgery elsewhere 14 years back without any visual gain. Right eye demonstrated no perception of light and the best corrected visual acuity in the left eye was 6/6, N6...
February 9, 2018: BMC Ophthalmology
https://www.readbyqxmd.com/read/29421548/bacteria-responsive-intelligent-wound-dressing-simultaneous-in-situ-detection-and-inhibition-of-bacterial-infection-for-accelerated-wound-healing
#13
Jin Zhou, Danyu Yao, Zhiyong Qian, Sen Hou, Linhao Li, A Tobias A Jenkins, Yubo Fan
The evolved resistance of antibiotics exhibited by some dreaded clinical pathogens and formation of biofilms has caused life-threatening problems for patients with burns and other wounds. Here, in order to avoid antibiotic overuse, and thus decreasing the occurrence of antimicrobial-resistant bacteria, a theranostic wound dressing, composed of biocompatible UV-photocrosslinkable methacrylated gelatin (GelMA) encapsulating both antimicrobial and fluorescent vesicles, has been developed. The system can respond to the microbiological environment of the wound via a simple color change and antimicrobials release only when require and this is in essence passive as they do not respond to their local environments and benign bacteria, and only operates when pathogenic bacteria exist...
April 2018: Biomaterials
https://www.readbyqxmd.com/read/29414444/correlative-microscopy-for-structural-microbiology
#14
REVIEW
Stuart C Howes, Roman I Koning, Abraham J Koster
Understanding how microbes utilize their environment is aided by visualizing them in their natural context at high resolution. Correlative imaging enables efficient targeting and identification of labelled viral and bacterial components by light microscopy combined with high resolution imaging by electron microscopy. Advances in genetic and bioorthogonal labelling, improved workflows for targeting and image correlation, and large-scale data collection are increasing the applicability of correlative imaging methods...
January 29, 2018: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29388184/imaging-the-unimaginable-desorption-electrospray-ionization-imaging-mass-spectrometry-desi-ims-in-natural-product-research
#15
Delphine Parrot, Stefano Papazian, Daniel Foil, Deniz Tasdemir
Imaging mass spectrometry (IMS) has recently established itself in the field of "spatial metabolomics." Merging the sensitivity and fast screening of high-throughput mass spectrometry with spatial and temporal chemical information, IMS visualizes the production, location, and distribution of metabolites in intact biological models. Since metabolite profiling and morphological features are combined in single images, IMS offers an unmatched chemical detail on complex biological and microbiological systems. Thus, IMS-type "spatial metabolomics" emerges as a powerful and complementary approach to genomics, transcriptomics, and classical metabolomics studies...
January 31, 2018: Planta Medica
https://www.readbyqxmd.com/read/29384903/outpatient-treatment-of-acute-bacterial-skin-and-skin-structure-infections-absssi-with-tedizolid-phosphate-and-linezolid-in-patients-in-the-united-states-subgroup-analysis-of-2-randomized-phase-3-trials
#16
Carisa De Anda, Steven Anuskiewicz, Philippe Prokocimer, Jose Vazquez
BACKGROUND: Acute bacterial skin and skin structure infections (ABSSSI) are a frequent cause of hospital admissions in the United States. Safe and effective outpatient treatments may lower ABSSSI-associated health care costs by reducing unnecessary hospital admissions. Using data from 2 phase 3 trials (ESTABLISH-1, NCT01170221; ESTABLISH-2, NCT01421511), this post-hoc analysis explored the efficacy and safety of tedizolid in an outpatient setting. METHODS: Subgroup analysis was performed on US outpatients (defined as patients who were not in hospital at the time of treatment initiation) with ABSSSI caused by presumed or proven gram-positive pathogens...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29380771/ocular-manifestations-of-isolated-corneal-bee-sting-injury-management-strategies-and-clinical-outcomes
#17
Hemalatha Gudiseva, Madhu Uddaraju, Sayali Pradhan, Manoranjan Das, Jeena Mascarenhas, M Srinivasan, N Venkatesh Prajna
PURPOSE: The purpose of this study is to describe the presenting features, management strategies, and clinical outcome following bee sting injury to the cornea. METHODS: Retrospective case series involving 11 eyes of 11 patients with corneal bee sting injuries who presented over a period of 2 years. Nine of these 11 eyes had the presence of intact bee stinger in the cornea, which was removed immediately under an operating microscope and sent for microbiological and histopathological evaluation...
February 2018: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29371064/metagenomic-analysis-for-detecting-fusarium-solani-in-a-case-of-fungal-keratitis
#18
Chika Shigeyasu, Masakazu Yamada, Kotaro Aoki, Yoshikazu Ishii, Kazuhiro Tateda, Takashi Yaguchi, Yukinobu Okajima, Yuichi Hori
PURPOSE: To report a difficult-to-identify case of keratitis due to Fusarium solani, diagnosed with the help of exhaustive gene analysis. CASE: A 47-year-old woman attended our hospital with a refractory corneal ulcer associated with contact lens wear in her left eye that had appeared two weeks earlier. On her initial visit, slit lamp examination revealed a small double-ringed irregular hyphate ulcer in the center of the cornea, which had no epithelial defect, suggesting fungal infection...
January 20, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29369229/stenotrophomonas-maltophilia-a-case-series-of-a-rare-keratitis-affecting-patients-with-bandage-contact-lens
#19
Henry D J Hogg, We Fong Siah, Arthur Okonkwo, Manjusha Narayanan, Francisco C Figueiredo
OBJECTIVES: Stenotrophomonas maltophilia is an opportunistic pathogen known to form biofilms on contact lens and case surfaces that may result in permanent visual loss in cases of microbial keratitis. Because of its multiple drug resistance and extremely low incidence, there is little consensus on treatment. We investigated the predisposing factors, management, and visual outcomes in a small case series of patients to better inform the management of this rarely reported keratitis. METHODS: Retrospective analysis of medical records was performed at a single tertiary referral center between 2011 and 2017...
January 22, 2018: Eye & Contact Lens
https://www.readbyqxmd.com/read/29353020/ultrasound-improves-antimicrobial-effect-of-sodium-dichloroisocyanurate-to-reduce-salmonella-typhimurium-on-purple-cabbage
#20
Ana Lúcia Almeida Duarte, Denes Kaic Alves do Rosário, Syllas Borburema Silva Oliveira, Hygor Lendell Silva de Souza, Raquel Vieira de Carvalho, Joel Camilo Souza Carneiro, Pollyanna Ibrahim Silva, Patrícia Campos Bernardes
The consumer's interest in ready-to-eat, fast-ready, nutritious and fresh foods is a major challenge for the food industry. Thus, studies on new sanitization methods are relevant. The effect of Sodium Hypochlorite (SH), Benzalkonium Chloride (BC), and Sodium Dichloroisocyanurate (SD) isolated or combined with Ultrasound (US) in purple cabbage quality over 7 days of storage at 8 ± 1 °C was evaluated. The natural microbiota (mesophilic aerobic bacteria, lactic acid bacteria and coliforms), intentionally inoculated Salmonella Typhimurium, physicochemical quality (anthocyanins, pH, total titratable acidity, instrumental color and mass loss) and sensorial quality (multiple comparison test and visual sensory acceptance) were analyzed...
March 23, 2018: International Journal of Food Microbiology
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