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serin protease inhibitor

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https://www.readbyqxmd.com/read/28315440/optimized-production-and-characterization-of-a-detergent-stable-protease-from-lysinibacillus-fusiformis-c250r
#1
REVIEW
Sondes Mechri, Mouna Kriaa, Mouna Ben Elhoul Berrouina, Maroua Omrane Benmrad, Nadia Zaraî Jaouadi, Hatem Rekik, Khelifa Bouacem, Amel Bouanane-Darenfed, Alif Chebbi, Sami Sayadi, Mohamed Chamkha, Samir Bejar, Bassem Jaouadi
In this study, we aimed to optimize the cultural and nutritional conditions for protease production by Lysinibacillus fusiformis strain C250R in submerged fermentation process using statistical methodology. The most significant factors (gruel, wheat bran, yeast extract, and FeSO4) were identified by Plackett-Burman design. Response surface methodology (RSM) was used to determine the optimum levels of the screened factors and their interaction. Under the optimized conditions, protease yield 3100U/mL was 4.5 folds higher than those obtained by the use of the initial conditions (680U/ml)...
March 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28303356/the-association-of-plasminogen-activator-inhibitor-type-1-pai-1-level-and-pai-1-4g-5g-gene-polymorphism-with-the-formation-and-the-grade-of-endometrial-cancer
#2
Malik Ejder Yıldırım, Savas Karakuş, Hande Küçük Kurtulgan, Hasan Kılıçgün, Serpil Erşan, Sevtap Bakır
Plasminogen activator inhibitor type 1 (PAI-1) is a serine protease inhibitor (Serpine 1), and it inhibits both tissue plasminogen activator and urokinase plasminogen activator which are important in fibrinolysis. We aimed to find whether there is a possible association between PAI-1 level, PAI-1 4G/5G polymorphism, and endometrial cancer. PAI-1 levels in peripheral blood were determined in 82 patients with endometrial carcinoma and 76 female healthy controls using an enzyme-linked immunoassay (ELISA). Then, the genomic DNA was extracted and screened by reverse hybridization procedure (Strip assay) to detect PAI 1 4G/5G polymorphism...
March 16, 2017: Biochemical Genetics
https://www.readbyqxmd.com/read/28296156/pai1-a-novel-pp1-interacting-protein-that-mediates-human-plasma-s-anti-apoptotic-effect-in-endothelial-cells
#3
Hui Yao, Guangchun He, Chao Chen, Shichao Yan, Lu Lu, Liujiang Song, K Vinod Vijayan, Qinglong Li, Li Xiong, Xiongying Miao, Xiyun Deng
Activation of apoptotic signalling in endothelial cells contributes to the detrimental effects of a variety of pathological stimuli. In investigating the molecular events underlying the anti-apoptotic effect of human plasma in cultured human endothelial cells, we unexpectedly uncovered a novel mechanism of apoptosis suppression by human plasma through an interaction between two previously unrelated proteins. Human plasma inhibited hypoxia-serum deprivation-induced apoptosis and stimulated BAD(S136) and Akt(S473) phosphorylation...
March 11, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28289587/polymorphisms-of-%C3%AE-1-antitrypsin-and-interleukin-6-genes-and-the-progression-of-hepatic-cirrhosis-in-patients-with-a-hepatitis-c-virus-infection
#4
T Motawi, O G Shaker, R M Hussein, M Houssen
Hepatitis C virus (HCV) infection represents a serious health problem. The -174 G/C mutation in the pro inflammatory cytokine interleukin-6 (IL-6) is associated with developing liver diseases. Likewise, the S and Z mutations in the serine protease inhibitor α1-antitrypsin (A1AT) are associated with pulmonary emphysema and/or liver cirrhosis. We explored the distribution of the single nucleotide polymorphisms (SNPs) of IL-6 and A1AT genes in chronic HCV-infected patients and evaluated their impact on the progression of liver cirrhosis...
December 1, 2016: Balkan Journal of Medical Genetics: BJMG
https://www.readbyqxmd.com/read/28283430/identification-of-a-small-pacifastin-protease-inhibitor-from-nasonia-vitripennis-venom-that-inhibits-humoral-immunity-of-host-musca-domestica
#5
Cen Qian, Dan Liang, Ya Liu, Pei Wang, Saima Kausar, Guoqing Wei, Baojian Zhu, Lei Wang, Chaoliang Liu
Nasonia vitripennis is an important natural enemy of many flies. Pacifastin protease inhibitors (PPIs) play an important role in development and innate immunity of insects. In this study, cDNA sequences of a small pacifastin protease inhibitor in N. vitripennis (NvSPPI) was characterized and its open reading frame (ORF) contains 243bp. Real-time quantitative PCR (RT-qPCR) results revealed that NvSPPI mRNA were detected specifically in the venom apparatus, while they were expressed at low levels in other tissues tested...
March 7, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28278112/purification-and-characterization-of-a-novel-trypsin-like-protease-from-green-seeded-chickpea-cicer-arientum
#6
Tooba Naz Shamsi, Romana Parveen, Priyankar Sen, Sadaf Fatima
The present study describes the purification and physicochemical and biochemical characterization of trypsin-like protease from green-seeded chickpea (Cicer arientum). The crude extract of chickpea trypsin (CpT) was obtained by homogenization followed by differential ammonium sulfate precipitation. The CpT was purified by ion-exchange chromatography on diethylaminoethyl (DEAE) column, pre-equilibrated with 20 mM tris-CaCl2 buffer (pH 8.2) with a flow rate of 0.5 mL min(-1). The molecular weight and purity of ∼23 kDa of CpT were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis...
February 10, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28273904/methionine-aminopeptidase-2-is-a-key-regulator-of-apoptotic-like-cell-death-in-leishmania-donovani
#7
Ritesh Kumar, Kartikeya Tiwari, Vikash Kumar Dubey
We investigate the role of methionine aminopeptidase 2 (MAP2) in miltefosine induced programmed cell death (PCD) in promastigote form of L. donovani. We report that TNP-470, an inhibitor of MAP2, inhibits programmed cell death in miltefosine treated promastigotes. It inhibits the biochemical features of metazoan apoptosis, including caspase3/7 protease like activity, oligonucleosomal DNA fragmentation, collapse of mitochondrial transmembrane potential, and increase in cytosolic pool of calcium ions but did not prevent the cell death and phosphatidyl serine externalization...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28270225/post-transcriptional-regulation-of-fruit-ripening-and-disease-resistance-in-tomato-by-the-vacuolar-protease-slvpe3
#8
Weihao Wang, Jianghua Cai, Peiwen Wang, Shiping Tian, Guozheng Qin
BACKGROUND: Proteases represent one of the most abundant classes of enzymes in eukaryotes and are known to play key roles in many biological processes in plants. However, little is known about their functions in fruit ripening and disease resistance, which are unique to flowering plants and required for seed maturation and dispersal. Elucidating the genetic mechanisms of fruit ripening and disease resistance is an important goal given the biological and dietary significance of fruit. RESULTS: Through expression profile analyses of genes encoding tomato (Solanum lycopersicum) cysteine proteases, we identify a number of genes whose expression increases during fruit ripening...
March 7, 2017: Genome Biology
https://www.readbyqxmd.com/read/28262971/the-opportunity-of-precision-medicine-for-breast-cancer-with-context-sensitive-tumor-suppressor-maspin
#9
Margarida M Bernardo, Sijana H Dzinic, Maria J Matta, Ivory Dean, Lina Saker, Shijie Sheng
To improve the precision of molecular diagnosis and to develop and guide targeted therapies of breast cancer, it is essential to determine the mechanisms that underlie the specific tumor phenotypes. To this end, the application of a snapshot of gene expression profile for breast cancer diagnosis and prognosis is fundamentally challenged since the tissue-based data are derived from heterogonous cell types and are not likely to reflect the dynamics of context-dependent tumor progression and drug sensitivity. The intricate network of epithelial differentiation program can be concertedly controlled by tumor suppressor maspin, a homologue of clade B serine protease inhibitors (serpin), through its multifaceted molecular interactions in multiple subcellular localizations...
March 6, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28260498/treatment-with-proprotein-convertase-subtilisin-kexin-type-9-pcsk9-inhibitors-to-reduce-cardiovascular-inflammation-and-outcomes
#10
Aldo Bonaventura, Federico Carbone, Alessandra Vecchié, Franco Dallegri, Giovanni G Camici, Fabrizio Montecucco, Luca Liberale
Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) is a serine protease involved in cholesterol homeostasis. After binding to the complex low-density lipoprotein (LDL)-receptor, PCSK9 induces its intracellular degradation, thus reducing serum LDL clearance. PCSK9 is mainly secreted by the liver, but it is also expressed to a lesser extent in other organs. Apart from the well-known activity concerning hepatic LDL receptor-mediated pathway, PCSK9 has been supposed to potentially interfere with vascular inflammation in atherogenesis...
March 3, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28258200/endogenous-natural-complement-inhibitor-regulates-cardiac-development
#11
Simon A Mortensen, Louise L Skov, Kasper Kjaer-Sorensen, Annette G Hansen, Søren Hansen, Frederik Dagnæs-Hansen, Jens C Jensenius, Claus Oxvig, Steffen Thiel, Søren E Degn
Congenital heart defects are a major cause of perinatal mortality and morbidity, affecting >1% of all live births in the Western world, yet a large fraction of such defects have an unknown etiology. Recent studies demonstrated surprising dual roles for immune-related molecules and their effector mechanisms during fetal development and adult homeostasis. In this article, we describe the function of an endogenous complement inhibitor, mannan-binding lectin (MBL)-associated protein (MAp)44, in regulating the composition of a serine protease-pattern recognition receptor complex, MBL-associated serine protease (MASP)-3/collectin-L1/K1 hetero-oligomer, which impacts cardiac neural crest cell migration...
March 3, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28248990/a-transcriptomic-survey-of-migdolus-fryanus-sugarcane-rhizome-borer-larvae
#12
Darlan Gonçalves Nakayama, Célio Dias Santos Júnior, Luciano Takeshi Kishi, Rafael Pedezzi, Adelita Carolina Santiago, Andrea Soares-Costa, Flavio Henrique-Silva
Sugarcane, a major crop grown in the tropical and subtropical areas of the world, is produced mainly for sucrose, which is used as a sweetener or for the production of bioethanol. Among the numerous pests that significantly affect the yield of sugarcane, the sugarcane rhizome borer (Migdolus fryanus, a cerambycidae beetle) is known to cause severe damage to the crops in Brazil. The absence of molecular information about this insect reinforces the need for studies and an effective method to control this pest...
2017: PloS One
https://www.readbyqxmd.com/read/28245763/recent-advances-of-hepsin-targeted-inhibitors
#13
Hongmok Kwon, JooYeon Han, Ki-Yong Lee, Sang-Hyun Son, Youngjoo Byun
Hepsin is a type II transmembrane serine protease (TTSP) that plays a crucial role in cell growth and development. Hepsin is highly expressed in prostate cancer (PCa) and associated with its progression and metastasis. Therefore, it has been considered as an attractive biomarker of PCa. Recently, low molecular weight inhibitors targeting hepsin have been developed. Based on the key chemical scaffold, they can be classified into four classes: Indolecarboxamidines, benzamidines, peptide-based analogs, and 2,3-dihydro-1H-perimidines...
February 27, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28243911/effect-of-sulfation-and-molecular-weight-on-anticoagulant-activity-of-dextran
#14
N N Drozd, Yu S Logvinova, M A Torlopov, E V Udoratina
Sulfation (to 2.8) of dextrans with molecular weight of 150 and 20 kDa was followed by the appearance of anticoagulant activity that increased with decreasing their molecular weight and did not depend on antithrombin, plasma inhibitor of serine proteases of the blood coagulation system. Antithrombin activity of dextran sulfate with a molecular weight of 20 kDa reached 12.6-15.3 U/mg. Dextran sulfates with molecular weights of 20 and 150 kDa did not potentiate ADP-induced human platelet aggregation.
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28242925/serine-protease-inhibitors-from-squid-ovary-extraction-and-its-effect-on-proteolysis-and-gel-properties-of-surimi
#15
Avtar Singh, Soottawat Benjakul
Serine protease inhibitors from squid ovary (SOSPI) were extracted using different extraction media and conditions. Optimal condition included 0.45 M NaCl for 1 h. After heat treatment at 70 °C for 10 min, the highest specific activity was obtained. Based on Sephadex G-75 gel filtration and activity staining, the major SOSPIs were present as monomer with molecular weight of 9.10 and 10.27 kDa. Impact of SOSPI on autolysis of bigeye snapper surimi was studied. Myosin heavy chain was more retained with coincidentally lower trichloroacetic acid-soluble peptide content as the level of SOSPI increased...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28242427/purification-and-characterization-of-a-collagenase-from-penicillium-sp-ucp-1286-by-polyethylene-glycol-phosphate-aqueous-two-phase-system
#16
Maria Carolina de Albuquerque Wanderley, José Manoel Wanderley Duarte Neto, Wendell Wagner Campos Albuquerque, Daniela de Araújo Viana Marques, Carolina de Albuquerque Lima, Sara Isabel da Cruz Silvério, José Luiz de Lima Filho, José António Couto Teixeira, Ana Lúcia Figueiredo Porto
Collagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including low-processing time, low-cost material and low-energy consumption. The collagenase produced by Penicillium sp...
February 24, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28238878/characterization-and-expression-analysis-of-serpinb3-the-first-clade-b-serine-protease-inhibitor-in-pacific-white-shrimp-litopenaeus-vannamei
#17
Yongjie Liu, Fujun Hou, Xiaolin Liu
Currently, about nine serpin clades (A-I) were preferentially observed in higher animals and clustered on the basis of function. Of these, eight clades contain extracellular proteins, while clade B contains predominantly intracellular proteins. In the present study, the first clade B serpin (named LvserpinB3) was identified from the Pacific white shrimp Litopenaeus vannamei. LvserpinB3 encoded a 412-amino acid protein with a 19-amino acid signal peptide and a serpin domain. Moreover, a transmembrane helix (TMHs) was predicted to be located on the N-terminal of LvserpinB3...
February 24, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28238440/the-proteolytic-system-of-pineapple-stems-revisited-purification-and-characterization-of-multiple-catalytically-active-forms
#18
André Matagne, Laetitia Bolle, Rachida El Mahyaoui, Danielle Baeyens-Volant, Mohamed Azarkan
Crude pineapple proteases extract (aka stem bromelain; EC 3.4.22.4) is an important proteolytic mixture that contains enzymes belonging to the cysteine proteases of the papain family. Numerous studies have been reported aiming at the fractionation and characterization of the many molecular species present in the extract, but more efforts are still required to obtain sufficient quantities of the various purified protease forms for detailed physicochemical, enzymatic and structural characterization. In this work, we describe an efficient strategy towards the purification of at least eight enzymatic forms...
February 23, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28238104/microbial-carcinogenic-toxins-and-dietary-anti-cancer-protectants
#19
REVIEW
Trevor W Stone, L Gail Darlington
Several toxins are known which account for the ability of some bacteria to initiate or promote carcinogenesis. These ideas are summarised and evidence is discussed for more specific mechanisms involving chymotrypsin and the bacterial chymotryptic enzyme subtilisin. Subtilisin and Bacillus subtilis are present in the gut and environment and both are used commercially in agriculture, livestock rearing and meat processing. The enzymes deplete cells of tumour suppressors such as deleted in colorectal cancer (DCC) and neogenin, so their potential presence in the food chain might represent an important link between diet and cancer...
February 25, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28236540/a-critical-review-on-serine-protease-key-immune-manipulator-and-pathology-mediator
#20
REVIEW
S Patel
Proteolytic activity is fundamental to survival, so it is not surprising that all living organisms have proteases, especially seine protease. This enzyme in its numerous isoforms and homologues, constitutes the quintessential offence and defence factors, in the form of surface proteins, secreted molecules, gut digestive enzymes, venom in specialised glands or plant latex, among other manifestations. Occurring as trypsin, chymotrypsin, elastase, collagenase, thrombin, subtilisin etc., it mediates a diverse array of functions, including pathological roles as inflammatory, coagulatory to haemorrhagic...
February 21, 2017: Allergologia et Immunopathologia
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