keyword
MENU ▼
Read by QxMD icon Read
search

serin protease inhibitor

keyword
https://www.readbyqxmd.com/read/29452445/suppressive-role-of-tissue-factor-pathway-inhibitor-%C3%AE-in-platelet-dependent-fibrin-formation-under-flow-is-restricted-to-low-procoagulant-strength
#1
Stella Thomassen, Tom G Mastenbroek, Frauke Swieringa, Kristien Winckers, Marion A H Feijge, Roy Schrijver, Judith M E M Cosemans, Susan A Maroney, Alan E Mast, Tilman M Hackeng, Johan W M Heemskerk
Tissue factor pathway inhibitor-alpha (TFPI-α) is a Kunitz-type serine protease inhibitor, which suppresses coagulation by inhibiting the tissue factor (TF)/factor VIIa complex as well as factor Xa. In static plasma-phospholipid systems, TFPI-α thus suppresses both factor Xa and thrombin generation. In this article, we used a microfluidics approach to investigate how TFPI-α regulates fibrin clot formation in platelet thrombi at low wall shear rate. We therefore hypothesized that the anticoagulant effect of TFPI-α in plasma is a function of the local procoagulant strength-defined as the magnitude of thrombin generation under flow, due to local activities of TF/factor VIIa and factor Xa...
February 16, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29451201/tackling-dipeptidyl-peptidase-iv-in-neurological-disorders
#2
REVIEW
Ghaith Al-Badri, Gian Marco Leggio, Giuseppe Musumeci, Rubina Marzagalli, Filippo Drago, Alessandro Castorina
Dipeptidyl peptidase IV (DPP-IV) is a serine protease best known for its role in inactivating glucagon-like peptide-1 (GLP-1), pituitary adenylate cyclase-activating polypeptide (PACAP) and glucose-dependent insulinotropic peptide (GIP), three stimulators of pancreatic insulin secretion with beneficial effects on glucose disposal. Owing to the relationship between DPP-IV and these peptides, inhibition of DPP-IV enzyme activity is considered as an attractive treatment option for diabetic patients. Nonetheless, increasing studies support the idea that DPP-IV might also be involved in the development of neurological disorders with a neuroinflammatory component, potentially through its non-incretin activities on immune cells...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29448066/single-step-purification-via-magnetic-nanoparticles-of-new-broad-ph-active-protease-from-penicillium-aurantiogriseum
#3
José Manoel Wanderley Duarte Neto, Maria Carolina de Albuquerque Wanderley, Carolina de Albuquerque Lima, Ana Lúcia Figueiredo Porto
A new set of applications can be achieved when using high stability proteases. Industrially, high costs can be related to production medium and purification process. Magnetic nanoparticles have been successfully used for rapid and scalable purification. In this work, azocasein were immobilized on magnetite nanoparticles and applied in a single step purification of protease produced by Penicillium aurantiogriseum using soybean flour medium, and the new purified enzyme was characterized. Glutaraldehyde activated nanoparticles were used in azocasein immobilization and then incubated with dialyzed 60-80% saline precipitation fraction of crude extract for purification...
February 12, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29442501/exploiting-the-s4-s5-specificity-of-human-neutrophil-proteinase-3-to-improve-the-potency-of-peptidyl-di-chlorophenyl-phosphonate-ester-inhibitors-a-kinetic-and-molecular-modeling-analysis
#4
Carla Guarino, Natalia Gruba, Renata Grzywa, Edyta Dyguda-Kazimierowicz, Yveline Hamon, Monika Legowska, Marcin Skoreński, Sandrine Dallet-Choisy, Sylvain Marchand-Adam, Christine Kellenberger, Dieter E Jenne, Marcin Sieńczyk, Adam Lesner, Francis Gauthier, Brice Korkmaz
The neutrophilic serine protease proteinase 3 (PR3) is involved in inflammation and immune response and thus appears as a therapeutic target for a variety of infectious and inflammatory diseases. Here we combined kinetic and molecular docking studies to increase the potency of peptidyl-diphenyl phosphonate PR3 inhibitors. Occupancy of the S1 subsite of PR3 by a nVal residue and of the S4-S5 subsites by a biotinylated Val residue as obtained in biotin-VYDnVP(O-C6H4-4-Cl)2 enhanced the second order inhibition constant kobs/[I] towards PR3 by more than ten times (kobs/[I] = 73...
February 14, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29440562/myb52-negatively-regulates-pectin-demethylesterification-in-seed-coat-mucilage
#5
Dachuan Shi, Angyan Ren, Xianfeng Tang, Guang Qi, Zongchang Xu, Guohua Chai, Ruibo Hu, Gongke Zhou, Yingzhen Kong
Pectins are synthesized and methyl-esterified in the Golgi apparatus and then demethylesterified by pectin methylesterases (PMEs) located in the cell wall. The degree of methylesterification affects the functional properties of pectin, and thereby influences plant growth and development, as well as defense responses. However, little is known about the mechanisms that regulate pectin demethylesterification. Here, we show that in seed coat mucilage the absence of the MYB52 transcription factor is correlated with an increase in PME activity and a decrease in the degree of pectin methylesterification...
February 9, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29439245/downregulation-of-spink13-promotes-metastasis-by-regulating-upa-in-ovarian-cancer-cells
#6
Shengyun Cai, Pei Zhang, Suhe Dong, Li Li, Jianming Cai, Mingjuan Xu
BACKGROUND/AIMS: Ovarian cancer (OC) is the fifth leading cause of cancer-related death in women, and it is difficult to diagnose at an early stage. The purpose of this study was to explore the prognostic biological markers of OC. METHODS: Univariate Cox regression analysis was used to identify genes related to OC prognosis from the Cancer Genome Atlas(TCGA) database. Immunohistochemistry was used to analyse the level of SPINK13 in OC and normal tissues. Cell proliferation, apoptosis and invasion were performed using MTT assay, flow cytometric analysis and Transwell assay, respectively...
February 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29438412/tissue-distribution-and-subcellular-localizations-determine-in-vivo-functional-relationship-among-prostasin-matriptase-hai-1-and-hai-2-in-human-skin
#7
Shiao-Pieng Lee, Chen-Yu Kao, Shun-Cheng Chang, Yi-Lin Chiu, Yen-Ju Chen, Ming-Hsing G Chen, Chun-Chia Chang, Yu-Wen Lin, Chien-Ping Chiang, Jehng-Kang Wang, Chen-Yong Lin, Michael D Johnson
The membrane-bound serine proteases prostasin and matriptase and the Kunitz-type protease inhibitors HAI-1 and HAI-2 are all expressed in human skin and may form a tightly regulated proteolysis network, contributing to skin pathophysiology. Evidence from other systems, however, suggests that the relationship between matriptase and prostasin and between the proteases and the inhibitors can be context-dependent. In this study the in vivo zymogen activation and protease inhibition status of matriptase and prostasin were investigated in the human skin...
2018: PloS One
https://www.readbyqxmd.com/read/29437593/%C3%AE-1-antitrypsin-infusion-for-treatment-of-steroid-resistant-acute-graft-versus-host-disease
#8
John M Magenau, Steven C Goldstein, Dan Peltier, Robert J Soiffer, Thomas Braun, Attaphol Pawarode, Mary M Riwes, Maggi Kennel, Joseph H Antin, Corey S Cutler, Vincent T Ho, Edwin P Alyea, Brian L Parkin, Gregory A Yanik, Sung Won Choi, Eli C Lewis, Charles A Dinarello, John Koreth, Pavan Reddy
Corticosteroid resistance following acute GVHD (SR-aGVHD) results in high morbidity and mortality after allogeneic hematopoietic cell transplantation (HCT). Current immunosuppressive therapies for SR-aGVHD provide marginal effectiveness due to poor response or excessive toxicity, primarily from infection. Alpha-1 antitrypsin (AAT), a naturally abundant serine protease inhibitor, is capable of suppressing experimental GVHD by down-modulation of inflammation and increasing ratios of regulatory to effector T cells...
February 2, 2018: Blood
https://www.readbyqxmd.com/read/29434593/-leishmania-donovani-inhibitor-of-serine-peptidases-2-mediated-inhibition-of-lectin-pathway-and-upregulation-of-c5ar-signaling-promote-parasite-survival-inside-host
#9
Sudha Verma, Abhishek Mandal, Md Yousuf Ansari, Ajay Kumar, Kumar Abhishek, Ayan Kumar Ghosh, Ashish Kumar, Vinod Kumar, Sushmita Das, Pradeep Das
Leishmania donovani , the causative agent of Indian visceral leishmaniasis has to face several barriers of the immune system inside the mammalian host for its survival. The complement system is one of the first barriers and consists of a well-balanced network of proteases including S1A family serine proteases (SPs). Inhibitor of serine peptidases (ISPs) is considered as inhibitor of S1A family serine peptidases and is reported to be present in trypanosomes, including Leishmania . In our previous study, we have deciphered the role of ISPs [LdISP1 and L...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29432934/alpha-1-antitrypsin-deficiency-from-the-lung-to-the-heart
#10
Ivan Curjuric, Medea Imboden, Robert Bettschart, Seraina Caviezel, Julia Dratva, Marco Pons, Thomas Rothe, Arno Schmidt-Trucksäss, Daiana Stolz, Gian Andri Thun, Arnold von Eckardstein, Florian Kronenberg, Ilaria Ferrarotti, Nicole M Probst-Hensch
BACKGROUND AND AIMS: Alpha-1 antitrypsin (A1AT) is the most abundant serine protease inhibitor in human blood and exerts important anti-inflammatory and immune-modulatory effects. In combination with smoking or other long-term noxious exposures such as occupational dust and fumes, genetic A1AT deficiency can cause chronic obstructive pulmonary disease, a condition with elevated cardiovascular risk. The effects of A1AT deficiency on cardiovascular risk have hardly been studied today. METHODS: Using data from 2614 adults from the population-based SAPALDIA cohort, we tested associations of serum A1AT and SERPINA1 mutations with carotid intima-media thickness (CIMT, measured by B-mode ultrasonography) or self-reported arterial hypertension or cardiovascular disease in multiple regression models using a Mendelian Randomization like analysis design...
January 31, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29431273/hepatocyte-growth-factor-activator-inhibitors-hai-1-and-hai-2-emerging-key-players-in-epithelial-integrity-and-cancer
#11
REVIEW
Hiroaki Kataoka, Makiko Kawaguchi, Tsuyoshi Fukushima, Takeshi Shimomura
The growth, survival, and metabolic activities of multicellular organisms at the cellular level are regulated by intracellular signaling, systemic homeostasis and the pericellular microenvironment. Pericellular proteolysis has a crucial role in processing bioactive molecules in the microenvironment and thereby has profound effects on cellular functions. Hepatocyte growth factor activator inhibitor type 1 (HAI-1) and HAI-2 are type I transmembrane serine protease inhibitors expressed by most epithelial cells...
February 12, 2018: Pathology International
https://www.readbyqxmd.com/read/29428543/synergistic-strategies-of-predominant-toxins-in-snake-venoms
#12
REVIEW
Shengwei Xiong, Chunhong Huang
Synergism is a significant phenomenon present in snake venoms that may be an evolving strategy to potentiate toxicities. Synergism exists between different toxins or toxin complexes in various snake venoms, with phospholipaseA 2 s (PLA 2 s) (toxins or subunits) the main enablers. The predominant toxins, snake venom PLA 2 s, metalloproteases (SVMPs), serine proteases (SVSPs) and three-finger toxins (3FTxs), play essential roles in synergistic processes. The hypothetical mechanisms of synergistic effect can be generalized under the effects of amplification and chaperoning...
February 8, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29427715/two-i84-family-protease-inhibitors-from-chinese-razor-clams-sinonovacula-constricta-expressed-in-response-to-environmental-challenges
#13
Xiarong Wang, Qinggang Xue, Xiaowei Mao, Yinghui Dong, Chenhua Li, Zhihua Lin
Protease inhibitors play critical roles in numerous biological processes including host defense in all multicellular organisms. Eighty three evolutionary families of protease inhibitors are currently accommodated in the MEROPS database and the I84 family currently consists of 3 novel serine protease inhibitors from the eastern oyster Crassostrea virginica. In this study, we identified 2 new I84 family members from the Chinese razor clam Sinonovacula constricta, scSI-1 and scSI-2, using cDNA cloning and sequencing...
February 7, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29425229/schistosoma-mansoni-smki-1-serine-protease-inhibitor-binds-to-elastase-and-impairs-neutrophil-function-and-inflammation
#14
Suellen B Morais, Barbara C Figueiredo, Natan R G Assis, Debora M Alvarenga, Mariana T Q de Magalhães, Rafaela S Ferreira, Angélica T Vieira, Gustavo B Menezes, Sergio C Oliveira
Protease inhibitors have important function during homeostasis, inflammation and tissue injury. In this study, we described the role of Schistosoma mansoni SmKI-1 serine protease inhibitor in parasite development and as a molecule capable of regulating different models of inflammatory diseases. First, we determine that recombinant (r) SmKI-1 and its Kunitz domain but not the C-terminal region possess inhibitory activity against trypsin and neutrophil elastase (NE). To better understand the molecular basis of NE inhibition by SmKI-1, molecular docking studies were also conducted...
February 9, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29414786/the-serine-protease-htra1-contributes-to-the-formation-of-an-extracellular-25-kda-apolipoprotein-e-fragment-that-stimulates-neuritogenesis
#15
Sonia Sanz Muñoz, Hongyun Li, Kalani Ruberu, Qian Chu, Alan Saghatelian, Lezanne Ooi, Brett Garner
Apolipoprotein-E (apoE) is a glycoprotein highly expressed in the brain, where it appears to play a role in lipid transport, β-amyloid clearance, and neuronal signaling. ApoE proteolytic fragments are also present in the brain, but the enzymes responsible for apoE fragmentation are unknown, and the biological activity of specific apoE fragments remains to be determined. Here we utilised SK-N-SH neuroblastoma cells differentiated into neurons with all-trans retinoic acid (ATRA) to study extracellular apoE proteolysis...
February 2, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29413719/role-of-ulinastatin-a-trypsin-inhibitor-in-severe-acute-pancreatitis-in-critical-care-setting-a-retrospective-analysis
#16
Jui Yeshavant Lagoo, Moses Charles D'Souza, Anandajith Kartha, Appanervanda Muthanna Kutappa
PURPOSE: To evaluate the clinical utility of Ulinastatin, a multifunctional serine protease inhibitor, in the management of severe acute pancreatitis. MATERIALS AND METHODS: We conducted a retrospective analysis of the archived data of adult patients diagnosed with acute pancreatitis and admitted to surgical intensive care unit with one or more end organ dysfunction. The patients were divided into two groups depending on whether they did or did not receive ulinastatin...
January 25, 2018: Journal of Critical Care
https://www.readbyqxmd.com/read/29412815/dipeptidyl-peptidase-inhibitor-therapy-in-type-2-diabetes-control-of-the-incretin-axis-and-regulation-of-postprandial-glucose-and-lipid-metabolism
#17
REVIEW
Erin E Mulvihill
Dipeptidyl peptidase 4 (DPP4) is a widely expressed, serine protease which regulates the bioactivity of many peptides through cleavage and inactivation including the incretin hormones, glucagon like peptide -1 (GLP-1) and glucose dependent insulinotropic polypeptide (GIP). Inhibitors of DPP4 are used therapeutically to treat patients with Type 2 Diabetes Mellitus (T2DM) as they potentiate incretin action to regulate islet hormone secretion and improve glycemia and post-prandial lipid excursions. The widespread clinical use of DPP4 inhibitors has increased interest in the molecular mechanisms by which these drugs mediate their beneficial effects...
February 2018: Peptides
https://www.readbyqxmd.com/read/29412075/pcsk9-inhibition-for-ldl-lowering-and-beyond-implications-for-patients-with-peripheral-artery-disease
#18
Katrin Gebauer, Holger Reinecke
Low-density lipoprotein cholesterol (LDL-C) has been proven to be a causal factor of atherosclerosis and, along with other triggers like inflammation, the most frequent reason for peripheral arterial disease. Moreover, a linear correlation between LDL-C concentration and cardiovascular outcome in high-risk patients could be established during the past century. After the development of statins, numerous randomized trials have shown the superiority for LDL-C reduction and hence the decrease in cardiovascular outcomes including mortality...
February 7, 2018: VASA. Zeitschrift Für Gefässkrankheiten
https://www.readbyqxmd.com/read/29408179/kinetic-studies-of-serine-protease-inhibitors-in-simple-and-rapid-active-barrier-model-systems-diffusion-through-an-inhibitor-barrier
#19
Erika Billinger, Gunnar Johansson
A model based on gelatin for protease activity studies was designed. The model is also extended to study the efficiency of inhibitors in a separate protective layer covering the layer containing the target substrate. A good correlation between protease concentration and the size of erosion wells formed in a plain gelatin layer was observed. Similarly, increased concentration of inhibitors gave a systematic decrease in well area. Kinetic analyses of the two-layer model in a spectrophotometric plate reader with a fixed concentration of substrate in the bottom layer displayed a strict dependence of both inhibitor concentration and thickness of the top "protective" layer...
January 30, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29406699/multifunctional-serine-protease-inhibitor-coated-water-soluble-gold-nanoparticles-as-a-novel-targeted-approach-for-the-treatment-of-inflammatory-skin-diseases
#20
David Limon, Maria Jose Fabrega, Ana C Calpena, Josefa Badía, Laura Baldoma, Lluïsa Pérez-García
The overexpression and increased activity of the serine protease Kallikrein 5 (KLK5) is characteristic of inflammatory skin diseases such as Rosacea. The use of inhibitors of this enzyme - such as 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF·HCl) or the anti-human recombinant Kallikrein 5 (anti-KLK5) antibody - in the treatment of the disease has been limited due to their low bioavailability, for which their immobilization in drug delivery agents can contribute to making serine protease inhibitors clinically useful...
February 6, 2018: Bioconjugate Chemistry
keyword
keyword
88561
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"