keyword
MENU ▼
Read by QxMD icon Read
search

4EBP1

keyword
https://www.readbyqxmd.com/read/27899988/mtor-downstream-effectors-4ebp1-and-eif4e-are-overexpressed-and-associated-with-hpv-status-in-precancerous-lesions-and-carcinomas-of-the-uterine-cervix
#1
Aristidis Asimomytis, Maria Karanikou, Alexander Rodolakis, Anna Vaiopoulou, Paraskevi Tsetsa, George Creatsas, Theodoros Stefos, Aristidis Antsaklis, Efstratios Patsouris, George Z Rassidakis
The present study aims to investigate the expression levels of two critical mammalian target of rapamycin (mTOR) downstream effectors, 4E binding protein 1 (4EBP1) and eukaryotic initiation factor 4E (eIF4E) proteins, in precancerous squamous intraepithelial lesions and cancer of the uterine cervix, and their association with human papilloma virus (HPV) infection status. Uterine cervical biopsies from 73 patients were obtained, including 40 fresh-frozen samples and 42 archival formalin-fixed, paraffin-embedded tissue specimens...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27888132/vitamin-e-deficiency-depressed-fish-growth-disease-resistance-and-the-immunity-and-structural-integrity-of-immune-organs-in-grass-carp-ctenopharyngodon-idella-referring-to-nf-%C3%AE%C2%BAb-tor-and-nrf2-signaling
#2
Jia-Hong Pan, Lin Feng, Wei-Dan Jiang, Pei Wu, Sheng-Yao Kuang, Ling Tang, Yong-An Zhang, Xiao-Qiu Zhou, Yang Liu
This study investigated the effects of dietary vitamin E on growth, disease resistance and the immunity and structural integrity of head kidney, spleen and skin in grass carp (Ctenopharyngodon idella). The fish were fed six diets containing graded levels of vitamin E (0, 45, 90, 135, 180 and 225 mg/kg diet) for 10 weeks. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila. The results showed that compared with optimal vitamin E supplementation, vitamin E deficiency caused depressed growth, poor survival rates and increased skin lesion morbidity in grass carp...
November 22, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27832967/kinetic-modelling-of-in-vitro-data-of-pi3k-mtor1-pten-enzymes-and-on-target-inhibitors-rapamycin-bez235-and-ly294002
#3
Alexey Goltsov, Ghassan Tashkandi, Simon P Langdon, David J Harrison, James L Bown
The phosphatidylinositide 3-kinases (PI3K) and mammalian target of rapamycin-1 (mTOR1) are two key targets for anti-cancer therapy. Predicting the response of the PI3K/AKT/mTOR1 signalling pathway to targeted therapy is made difficult because of network complexities. Systems biology models can help explore those complexities but the value of such models is dependent on accurate parameterisation. Motivated by a need to increase accuracy in kinetic parameter estimation, and therefore the predictive power of the model, we present a framework to integrate kinetic data from enzyme assays into a unified enzyme kinetic model...
November 8, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27779207/hbv-core-promoter-mutations-and-akt-upregulate-s-phase-kinase-associated-protein-2-to-promote-postoperative-hepatocellular-carcinoma-progression
#4
Lubiao Chen, Lin Gu, Yurong Gu, Hongbo Wang, Meihai Deng, Zania Stamataki, Ye Htun Oo, Yuehua Huang
Mutations in the hepatitis B virus (HBV) core promoter (CP) have been shown to be associated with hepatocellular carcinoma (HCC). The CP region overlaps HBV X gene, which activates AKT to regulate hepatocyte survival. However, the cooperation between these two cascades in HCC progression remains poorly understood. Here, we assayed virological factors and AKT expression in liver tissues from 56 HCC patients with better prognoses (BHCC, ≥5-year survival) and 58 with poor prognoses (PHCC, <5-year survival) after partial liver resection...
October 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27769069/non-immunosuppressive-triazole-based-small-molecule-induces-anticancer-activity-against-human-hormone-refractory-prostate-cancers-the-role-in-inhibition-of-pi3k-akt-mtor-and-c-myc-signaling-pathways
#5
Wohn-Jenn Leu, Sharada Prasanna Swain, She-Hung Chan, Jui-Ling Hsu, Shih-Ping Liu, Mei-Ling Chan, Chia-Chun Yu, Lih-Ching Hsu, Yen-Lin Chou, Wei-Ling Chang, Duen-Ren Hou, Jih-Hwa Guh
A series of triazole-based small molecules that mimic FTY720-mediated anticancer activity but minimize its immunosuppressive effect have been produced. SPS-7 is the most effective derivative displaying higher activity than FTY720 in anti-proliferation against human hormone-refractory prostate cancer (HRPC). It induced G1 arrest of cell cycle and subsequent apoptosis in thymidine block-mediated synchronization model. The data were supported by a decrease of cyclin D1 expression, a dramatic increase of p21 expression and an associated decrease in RB phosphorylation...
October 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/27729552/impaired-coordination-between-signaling-pathways-is-revealed-in-human-colorectal-cancer-using-single-cell-mass-cytometry-of-archival-tissue-blocks
#6
Alan J Simmons, Cherié R Scurrah, Eliot T McKinley, Charles A Herring, Jonathan M Irish, M Kay Washington, Robert J Coffey, Ken S Lau
Cellular heterogeneity poses a substantial challenge to understanding tissue-level phenotypes and confounds conventional bulk analyses. To analyze signaling at the single-cell level in human tissues, we applied mass cytometry using cytometry time of flight to formalin-fixed, paraffin-embedded (FFPE) normal and diseased intestinal specimens. This technique, called FFPE-DISSECT (disaggregation for intracellular signaling in single epithelial cells from tissue), is a single-cell approach to characterizing signaling states in embedded tissue samples...
October 11, 2016: Science Signaling
https://www.readbyqxmd.com/read/27699948/reduced-phosphorylation-of-ribosomal-protein-s6-is-associated-with-sensitivity-to-mek-inhibition-in-gastric-cancer-cells
#7
Yuka Hirashita, Yoshiyuki Tsukamoto, Kazuyoshi Yanagihara, Shoichi Fumoto, Naoki Hijiya, Chisato Nakada, Tomohisa Uchida, Keiko Matsuura, Masaaki Kodama, Tadayoshi Okimoto, Tsutomu Daa, Masataka Seike, Hidekatsu Iha, Kuniaki Shirao, Kazunari Murakami, Masatsugu Moriyama
Since gastric cancer (GC) is characterized by amplifications of receptor tyrosine kinases (RTK) and KRAS, targeting of the RTK/KRAS-downstream pathways could help to broaden the applicability of molecular targeted therapy for GC. Here, we assembled a panel of 48 GC cell lines and screened predictors of responsiveness to inhibition of the RAF/MEK/ERK pathway, one of the RTK/KRAS-downstream pathways. We found that GC cells with MET amplification or KRAS mutation, but not amplification, tended to be sensitive to MEK inhibition...
October 3, 2016: Cancer Science
https://www.readbyqxmd.com/read/27650250/effects-of-high-epa-and-high-dha-fish-oils-on-changes-in-signaling-associated-with-protein-metabolism-induced-by-hindlimb-suspension-in-rats
#8
Gabriel Nasri Marzuca-Nassr, Kaio Fernando Vitzel, Luís Gustavo De Sousa, Gilson M Murata, Amanda Rabello Crisma, Carlos Flores Rodrigues Junior, Phablo Abreu, Rosângela Pavan Torres, Jorge Mancini-Filho, Sandro M Hirabara, Philip Newsholme, Rui Curi
The effects of either eicosapentaenoic (EPA)- or docosahexaenoic (DHA)-rich fish oils on hindlimb suspension (HS)-induced muscle disuse atrophy were compared. Daily oral supplementations (0.3 mL/100 g b.w.) with mineral oil (MO) or high EPA or high DHA fish oils were performed in adult rats. After 2 weeks, the animals were subjected to HS for further 2 weeks. The treatments were maintained alongside HS At the end of 4 weeks, we evaluated: body weight gain, muscle mass and fat depots, composition of fatty acids, cross-sectional areas (CSA) of the soleus muscle and soleus muscle fibers, activities of cathepsin L and 26S proteasome, and content of carbonylated proteins in the soleus muscle...
September 2016: Physiological Reports
https://www.readbyqxmd.com/read/27624100/leucine-supplementation-stimulates-protein-synthesis-and-reduces-degradation-signal-activation-in-muscle-of-newborn-pigs-during-acute-endotoxemia
#9
Adriana D Hernandez-García, Daniel A Columbus, Rodrigo Manjarín, Hanh V Nguyen, Agus Suryawan, Renán A Orellana, Teresa A Davis
Sepsis disrupts skeletal muscle proteostasis and mitigates the anabolic response to leucine (Leu) in muscle of mature animals. We have shown that Leu stimulates muscle protein synthesis (PS) in healthy neonatal piglets. To determine if supplemental Leu can stimulate PS and reduce protein degradation (PD) signaling in neonatal muscle during endotoxemia, overnight-fasted neonatal pigs were infused for 8 h with LPS or saline while plasma amino acids, glucose, and insulin were maintained at fasting levels during pancreatic-substrate clamps...
October 1, 2016: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27618686/proline-starvation-induces-unresolved-er-stress-and-hinders-mtorc1-dependent-tumorigenesis
#10
Nisebita Sahu, Darlene Dela Cruz, Min Gao, Wendy Sandoval, Peter M Haverty, Jinfeng Liu, Jean-Philippe Stephan, Benjamin Haley, Marie Classon, Georgia Hatzivassiliou, Jeff Settleman
The role of essential amino acids in metabolic reprogramming of cancer cells is now well established, whereas the role of non-essential amino acids (NEAAs) in malignancy remains less clear. Here, we have identified an important role for the NEAA proline in the tumorigenic potential of a subset of cancer cells. By profiling a large panel of cancer cell lines, we observed that proline consumption and expression of proline biosynthesis enzymes were well correlated with clonogenic and tumorigenic potential. Moreover, proline starvation or inhibition of proline biosynthesis enzymes impaired clonogenic/tumorigenic potential...
September 3, 2016: Cell Metabolism
https://www.readbyqxmd.com/read/27613409/gcn2-and-eif2%C3%AE-phosphorylation-independent-but-atf4-dependent-induction-of-care-containing-genes-in-methionine-deficient-cells
#11
Kevin M Mazor, Martha H Stipanuk
Amino-acid deprivation is sensed by the eIF2α kinase GCN2. Under conditions of essential amino-acid limitation, GCN2 phosphorylates eIF2α, inhibiting the formation of a new ternary complex and hence mRNA translation initiation. While decreasing global mRNA translation, eIF2α phosphorylation also increases the translation of the integrated stress response (ISR) transcription factor ATF4, which increases the expression of many stress response genes that contain a C/EBP-ATF response element (CARE), including Atf4, 4Ebp1, Asns, and Chop...
December 2016: Amino Acids
https://www.readbyqxmd.com/read/27613305/decreased-activation-of-placental-mtor-family-members-is-associated-with-the-induction-of-intrauterine-growth-restriction-by-secondhand-smoke-in-the-mouse
#12
Camilo Mejia, Josh Lewis, Clinton Jordan, Juan Mejia, Connor Ogden, Troy Monson, Duane Winden, Marc Watson, Paul R Reynolds, Juan A Arroyo
Cigarette smoke is known to be a risk for the development of intrauterine growth restriction (IUGR). Our objective was to assess the effects of secondhand smoke (SHS) during pregnancy and to what extent it regulates the activation of mTOR family members and murine trophoblast invasion. Mice were treated to SHS for 4 days. Placental and fetal weights were recorded at the time of necropsy. Immunohistochemistry was used to determine the level of placental trophoblast invasion. Western blots were utilized to assess the activation of caspase 3, XIAP, mTOR, p70 and 4EBP1 in treated and control placental lysates...
September 9, 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27599148/alterations-in-braf-gene-and-enhanced-mtor-and-mapk-signaling-in-dysembryoplastic-neuroepithelial-tumors-dnts
#13
Aanchal Kakkar, Atreye Majumdar, Anupam Kumar, Manjari Tripathi, Pankaj Pathak, Mehar C Sharma, Vaishali Suri, Vivek Tandon, Sarat P Chandra, Chitra Sarkar
OBJECTIVE: Recently, BRAF V600E mutation, and activation of mTOR and MAPK pathways have been identified in various glial/glioneuronal tumors. Dysembryoplastic neuroepithelial tumors (DNTs) are epilepsy-associated glioneuronal neoplasms which have not been analyzed extensively in this respect. METHODS: Sequencing for BRAF V600E mutation, analysis of BRAF copy number by qRT-PCR, and immunohistochemistry for mTOR (p-S6, p-4EBP1) and MAPK (p-MAPK) pathways were performed...
August 28, 2016: Epilepsy Research
https://www.readbyqxmd.com/read/27577068/sirolimus-and-metformin-synergistically-inhibit-hepatocellular-carcinoma-cell-proliferation-and-improve-long-term-survival-in-patients-with-hcc-related-to-hepatitis-b-virus-induced-cirrhosis-after-liver-transplantation
#14
Chuan Shen, Chenghong Peng, Baiyong Shen, Zhecheng Zhu, Ning Xu, Tao Li, Junjie Xie
Immunosuppressive agents used postoperatively after liver transplantation (LT) for hepatocellular carcinoma (HCC) favor recurrence and metastasis. Therefore, new effective immunosuppressants are needed. This retrospective study assessed combined sirolimus and metformin on survival of HCC patients after LT. In 2001-2013, 133 HCC patients with LT were divided into four groups: sirolimus and metformin combination (Sir+Met), sirolimus monotherapy (Sir), other immunosuppressants in diabetes mellitus (DM) patients without metformin (No Sir with DM), and other immunosuppressants in patients without DM (No Sir without DM)...
August 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27565232/different-dietary-energy-intake-affects-skeletal-muscle-development-through-an-akt-dependent-pathway-in-dorper-%C3%A3-small-thin-tailed-crossbred-ewe-lambs
#15
J X Zhao, X D Liu, K Li, W Z Liu, Y S Ren, J X Zhang
The objective of this experiment was to investigate the mechanisms through which different levels of dietary energy affect postnatal skeletal muscle development in ewe lambs. Twelve Dorper × Small Thin-Tailed crossbred ewe lambs (100 d of age; 20 ± 0.5 kg BW) were selected randomly and divided into 2 groups in a completely randomized design. Animals were offered identical diets at 100% or 65% of ad libitum intake. Lambs were euthanized when BW in the ad libitum group reached 35 kg and the semitendinosus muscle was sampled...
October 2016: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/27539583/amino-acids-regulate-mtor-pathway-and-milk-protein-synthesis-in-a-mouse-mammary-epithelial-cell-line-is-partly-mediated-by-t1r1-t1r3
#16
YanHong Wang, JunQiang Liu, Hui Wu, XingTang Fang, Hong Chen, ChunLei Zhang
PURPOSE: The mechanism of dietary amino acids in regulating milk protein synthesis at the translational level is not well understood. Numerous studies have shown that the amino acid signal is transferred through the mammalian target of rapamycin (mTOR) pathway; however, the extracellular amino acid-sensing mechanism that activates mTOR complex 1 is unknown. We tested the hypotheses that the T1R1/T1R3 heterodimer functions as a direct sensor of the fed state and amino acid availability preceding the mTOR pathway and affects milk protein synthesis in mammary epithelial cells...
August 18, 2016: European Journal of Nutrition
https://www.readbyqxmd.com/read/27531477/-molecular-mechanisms-of-resistance-to-phosphatidyl-inositol-3-kinase-inhibitors-in-triple-negative-breast-cancer-cells
#17
W L Zhang, W J Ma, S Chen, X Z Wu, H R Zhang, J H Zhang
OBJECTIVE: To explore the molecular mechanisms of resistance to phosphatidyl inositol 3-kinase (PI3K) inhibitors in triple-negative breast cancer (TNBC) cells. METHODS: HCC70 cells (TNBC) were transfected with siFZD7, siWANT5B or siGSK3 using lipofectamine 2000 transfection reagent. The expression levels of key proteins of WNT/β-catenin and PI3K/AKT/mTOR pathways were determined by Western blot analysis. After HCC70, MCF-7 (ER-positive) and SK-BR3 (HER2-positive) cells were treated with PI3K/AKT/mTOR inhibitors, the inhibition rates of cell proliferation were measured by MTT assay, and half maximal inhibitory concentrations (IC50) were calculated...
August 2016: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/27507116/saffron-crocus-sativus-pretreatment-confers-cardioprotection-against-ischemia-reperfusion-injuries-in-isolated-rabbit-heart
#18
Moni Nader, Nathalie Chahine, Charelle Salem, Ramez Chahine
Restoration of blood flow to the ischemic myocardium is imperative to avoid demise of cardiomyocytes, but is paradoxically associated with irreversible damage to cardiac tissues due to the excessive generation of reactive oxygen species (ROS). We have previously reported that saffron, a natural antioxidant, attenuated ischemia-reperfusion (IR) injuries in vitro; however, its role in a meaningful cardiac recovery remains unknown. Here, we show that saffron supplement (oral administration for 6 weeks) reduced myocardial damage and restored cardiac function in an IR model of rabbit hearts...
December 2016: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/27470546/synergistic-cytotoxicity-of-biib021-with-triptolide-through-suppression-of-pi3k-akt-mtor-and-nf-%C3%AE%C2%BAb-signal-pathways-in-thyroid-carcinoma-cells
#19
Si Hyoung Kim, Jun Goo Kang, Chul Sik Kim, Sung-Hee Ihm, Moon Gi Choi, Hyung Joon Yoo, Seong Jin Lee
The effec.t of BIIB021, a novel heat shock protein 90 (hsp90) inhibitor, on survival of thyroid carcinoma cells has not been evaluated. In this study, the impact of BIIB021 alone or in combination with the histone acetyltransferase inhibitor triptolide on survival of thyroid carcinoma cells was identified. In 8505C and TPC-1 thyroid carcinoma cells, BIIB021 caused cell death in conjunction with alterations in expression of hsp90 client proteins. Cotreatment of both BIIB021 and triptolide, compared with treatment of BIIB021 alone, decreased cell viability, and increased the percentage of dead cells and cytotoxic activity...
June 18, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27430283/mtor-signaling-in-osteosarcoma-oncogenesis-and-therapeutic-aspects-review
#20
Kai Hu, Hai-Bo Dai, Zhi-Long Qiu
The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase that belongs to the phosphoinositide-3-kinase (PI3K)-related kinase family. Oncogenic activation of mTOR signaling significantly contributes to the progression of different types of cancers including osteosarcoma (OS; the most common primary malignant tumor of bone). In the present study, we review the association of the mTOR signaling pathway with OS, and the possible effective treatment strategies by targeting this pathway. In the metastatic behavior of OS, one of the most common actionable aberrations was found in the PI3K/Akt/mTOR pathway...
September 2016: Oncology Reports
keyword
keyword
88459
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"