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nitric oxide synthase

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https://www.readbyqxmd.com/read/29679858/anti-inflammatory-effect-of-apo-9-fucoxanthinone-via-inhibition-of-mapks-and-nf-kb-signaling-pathway-in-lps-stimulated-raw-264-7-macrophages-and-zebrafish-model
#1
Eun-A Kim, Seo-Young Kim, Bo-Ram Ye, Junseong Kim, Seok-Chun Ko, Won Woo Lee, Kil-Nam Kim, Il-Whan Choi, Won-Kyo Jung, Soo-Jin Heo
In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in in vitro RAW 264.7 cells and in vivo zebrafish model. In lipopolysaccharide (LPS)-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of inducible nitric oxide synthase (iNOS), suppressed cyclooxygenase-2 (COX-2) and an inflammatory cytokines; IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264...
April 18, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29679855/rutin-and-rutin-conjugated-gold-nanoparticles-ameliorate-collagen-induced-arthritis-in-rats-through-inhibition-of-nf-%C3%AE%C2%BAb-and-inos-activation
#2
Anum Gul, Bimal Kunwar, Maryam Mazhar, Shaheen Faizi, Dania Ahmed, Muhammad Raza Shah, Shabana U Simjee
Numerous studies have suggested that nuclear factor-κB (NF-κB) and inducible nitric oxide synthase (iNOS) are important mediators of inflammatory response in human and animal models of arthritis. Besides, oxidative stress markers, nitric oxide (NO) and peroxide (PO) are also major contributors in the pathogenesis of rheumatoid arthritis (RA). Over expression of these inflammatory mediators leads to the extracellular matrix degradation, and excessive cartilage and bone resorption, ultimately leading to the irreversible damage to joints...
April 18, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29678838/rho-associated-protein-kinase-1-mediates-the-regulation-of-inflammatory-markers-in-diabetic-retina-and-in-retinal-m%C3%A3-ller-cells
#3
Ghulam Mohammad, Heba Mowafak AlSharif, Mohammad Mairaj Siddiquei, Ajmal Ahmad, Kaiser Alam, Ahmed M Abu El-Asrar
GOAL: To investigate the effects of blocking Rho kinase pathway on the expression of inflammatory signaling pathways in the retina of diabetic mice and in human retinal Müller glial cells stimulated with high-glucose to replicate hyperglycemia. PROCEDURES: Retinas from diabetic mice and human retinal Müller glial cells (MIO-M1) were studied. Western blot analysis, immunofluorescence, and enzyme-linked immunosorbent assay were utilized to study the effect of the Rho kinase inhibitor fasudil on the expression of Rho-associated protein kinase-1 (ROCK-1), extracellular signal-regulated kinases1&2(ERK ½), phosphorylated nuclear factor kappa-light-chain-enhancer of activated B cells (p-NF-κB), inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1/CCL2)...
March 2018: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29677563/polysaccharide-of-hericium-erinaceus-attenuates-colitis-in-c57bl-6-mice-via-regulation-of-oxidative-stress-inflammation-related-signaling-pathways-and-modulating-the-composition-of-the-gut-microbiota
#4
Yilin Ren, Yan Geng, Yan Du, Wang Li, Zhen-Ming Lu, Hong-Yu Xu, Guo-Hua Xu, Jin-Song Shi, Zheng-Hong Xu
Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology. Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders. In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice. Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO)...
March 16, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29677454/combined-but-not-the-single-administration-of-vitamin-c-and-l-carnitine-ameliorates-cisplatin-induced-gastric-mucosa-damage-in-male-rats
#5
Modinat Adebukola Adefisayo, Wale Johnson Adeyemi, Quadri Kunle Alabi
Although cisplatin is a potent anticancer drug, it instigates oxidative and pro-inflammatory reactions which poses significant and distressing clinical symptoms in patients including nausea and vomiting which is related to damage of the gastric mucosa. This study investigated the effects of vitamin C and/or L-carnitine on cisplatin-induced gastric mucosa damage in rat. The rats were allocated into groups (n=5): a control group received distilled water and treatment groups received cisplatin (CIP) alone, cisplatin followed by vitamin C, L-carnitine or their combination...
April 20, 2018: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/29676937/effect-of-magnesium-acetyltaurate-and-taurine-on-endothelin1-induced-retinal-nitrosative-stress-in-rats
#6
Natasha Najwa Nor Arfuzir, Renu Agarwal, Igor Iezhitsa, Puneet Agarwal, Sabrilhakim Sidek, Alexander Spasov, Alexander Ozerov, Nafeeza Mohd Ismail
PURPOSE: Retinal ganglion cell apoptosis in glaucoma is associated with elevated levels of endothelin-1 (ET1), a potent vasoconstrictor. ET1-induced retinal ischemia leads to altered expression of nitric oxide synthase (NOS) isoforms leading to increased formation of nitric oxide (NO) and retinal nitrosative stress. Since magnesium (Mg) is known to improve endothelial functions and reduce oxidative stress and taurine (TAU) possesses potent antioxidant properties, we investigated the protective effects of magnesium acetyltaurate (MgAT) against ET1-induced nitrosative stress and retinal damage in rats...
April 20, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29676291/pancreatic-kininogenase-improves-erectile-function-in-streptozotocin-induced-type-2-diabetic-rats-with-erectile-dysfunction
#7
Guo-Tao Chen, Bai-Bing Yang, Jian-Huai Chen, Zheng Zhang, Lei-Lei Zhu, He-Song Jiang, Wen Yu, Yun Chen, Yu-Tian Dai
Erectile dysfunction (ED) associated with type 2 diabetes is a severe problem that requires effective treatment. Pancreatic kininogenase (PK) has the potential to improve the erectile function of ED patients. This study aims to investigate the effect of PK on erectile function in streptozotocin-induced type 2 diabetic ED rats. To achieve this goal, we divided male Sprague-Dawley rats into five groups. One group was not treated, and the other four groups were treated with saline, sildenafil, PK or sildenafil, and PK, respectively, for 4 weeks after the induction of type 2 diabetic ED...
April 20, 2018: Asian Journal of Andrology
https://www.readbyqxmd.com/read/29675883/aurantio-obtusin-an-anthraquinone-from-cassiae-semen-ameliorates-lung-inflammatory-responses
#8
Ki Sun Kwon, Ju Hee Lee, Kyung Su So, Byung Kyu Park, Hyun Lim, Jae Sue Choi, Hyun Pyo Kim
The purpose of the present study is to find the natural compound(s) having a therapeutic potential to treat lung inflammatory disorders. In our screening procedure, the methanol extract of the seeds of Cassia obtusifolia (cassiae semen) inhibited inducible nitric oxide synthase-catalyzed nitric oxide production in alveolar macrophages (MH-S). From the extract, 8 major anthraquinone derivatives were successfully isolated. They are chrysophanol, physcion, 2-hydroxy-emodin 1-methyl ether, obtusifolin, obtusin, aurantio-obtusin, chryso-obtusin, and gluco-obtusifolin, among which aurantio-obtusin (IC50  = 71...
April 20, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29675576/gintonin-mitigates-mptp-induced-loss-of-nigrostriatal-dopaminergic-neurons-and-accumulation-of-%C3%AE-synuclein-via-the-nrf2-ho-1-pathway
#9
Min Gi Jo, Muhammad Ikram, Myeung Hoon Jo, Lang Yoo, Kwang Chul Chung, Seung-Yeol Nah, Hongik Hwang, Hyewhon Rhim, Myeong Ok Kim
Gintonin, a ginseng-derived glycolipoprotein isolated from ginseng, has been shown to be neuroprotective in several neurological disorders such as Alzheimer's disease models and depressive-like behaviors. In this study, we sought to investigate the potential protective mechanisms of gintonin in an in vivo MPTP and in vitro MPP+ -mediated Parkinson's disease (PD) model. We hypothesized that activation of nuclear factor erythroid 2-related factor 2/heme oxygenase-1 (Nrf2/HO-1, potential therapeutic targets for neurodegeneration) with gintonin could abrogate PD-associated neurotoxicity by modulating the accumulation of α-synuclein, neuroinflammation, and apoptotic cell death in an MPTP/MPP+ models of PD...
April 19, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29675001/-leishmania-infantum-lipophosphoglycan-deficient-mutants-a-tool-to-study-host-cell-parasite-interplay
#10
Milena Lázaro-Souza, Christine Matte, Jonilson B Lima, Guillermo Arango Duque, Graziele Quintela-Carvalho, Áislan de Carvalho Vivarini, Sara Moura-Pontes, Cláudio P Figueira, Flávio H Jesus-Santos, Ulisses Gazos Lopes, Leonardo P Farias, Théo Araújo-Santos, Albert Descoteaux, Valéria M Borges
Lipophosphoglycan (LPG) is the major surface glycoconjugate of metacyclic Leishmania promastigotes and is associated with virulence in various species of this parasite. Here, we generated a LPG-deficient mutant of Leishmania infantum , the foremost etiologic agent of visceral leishmaniasis in Brazil. The L. infantum LPG-deficient mutant (Δ lpg1 ) was obtained by homologous recombination and complemented via episomal expression of LPG1 (Δ lpg1 + LPG1 ). Deletion of LPG1 had no observable effect on parasite morphology or on the presence of subcellular organelles, such as lipid droplets...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29674475/effect-of-sfrp5-secreted-frizzled-related-protein-5-on-the-wnt5a-wingless-type-family-member-5a-induced-endothelial-dysfunction-and-its-relevance-with-arterial-stiffness-in-human-subjects
#11
Yun Kyung Cho, Yu Mi Kang, Seung Eun Lee, Yoo La Lee, So Mi Seol, Woo Je Lee, Joong-Yeol Park, Chang Hee Jung
OBJECTIVE: SFRP5 (secreted frizzled-related protein 5) is an endogenous inhibitor of WNT5A (wingless-type family member 5a), which has been implicated in atherosclerosis. However, contradictory results have been reported about the role of SFRP5 in atherosclerosis. We aimed to investigate whether SFRP5 could restore WNT5A-induced endothelial dysfunction in vitro and ex vivo. In addition, we sought to determine whether the serum concentration of SFRP5 is associated with atherosclerosis in humans...
April 19, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29673954/oxidative-stress-in-osteoarthritis-and-antioxidant-effect-of-polysaccharide-from-angelica-sinensis
#12
Chao Zhuang, Yuji Wang, Yunkun Zhang, Nanwei Xu
This study aimed to investigate the oxidative stress in human osteoarthritis (OA) chondrocytes and the antioxidant effect of angelica sinensis polysaccharide (ASP) on it. Human OA chondrocytes and human normal chondrocytes induced by hydrogen peroxide (H2 O2 ) alone or treated with ASP were cultured, then they were measured with cell viability, levels of reactive oxygen species (ROS), nitric oxide (NO) and malondialdehyde (MDA) production, activities of inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD) and catalase (CAT), expression of related genes, such as collagen type II (Col2a1), Aggrecan, iNOS, SOD, CAT and peroxisome proliferator-activated receptor gamma (PPARγ)...
April 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29673643/use-of-gasotransmitters-for-the-controlled-release-of-polymer-based-nitric-oxide-carriers-in-medical-applications
#13
REVIEW
Chungmo Yang, Soohyun Jeong, Seul Ku, Kangwon Lee, Min Hee Park
Nitric Oxide (NO) is a small molecule gasotransmitter synthesized by nitric oxide synthase in almost all types of mammalian cells. NO is synthesized by NO synthase by conversion of l-arginine to l-citrulline in the human body. NO then stimulates soluble guanylate cyclase, from which various physiological functions are mediated in a concentration-dependent manner. High concentrations of NO induce apoptosis or antibacterial responses whereas low NO circulation leads to angiogenesis. The bidirectional effect of NO has attracted considerable attention, and efforts to deliver NO in a controlled manner, especially through polymeric carriers, has been the topic of much research...
April 16, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29673161/biflavonoids-isolated-from-selaginella-tamariscina-and-their-anti-inflammatory-activities-via-erk-1-2-signaling
#14
Sun-Yup Shim, Seul-Gi Lee, Mina Lee
Selaginella tamariscina ( S. tamariscina ) (Beauv.) Spring (Selaginellaceae) has been used in oriental medicine for the treatment of dysmenorrhea, chronic hepatitis, hyperglycemia, amenorrhea, hematuria, prolapse of the anus and metrorrhagia. In the present study, we isolated two strong anti-inflammatory compounds, the biflavonoids hinokiflavone (H) and 7′- O -methyl hinokiflavone (mH), from S. tamariscina and examined their anti-inflammatory activities in lipopolysaccharide (LPS)-mediated murine macrophages (RAW 264...
April 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29672270/role-of-atrial-natriuretic-peptide-in-controlling-diabetic-nephropathy-in-rats
#15
Lakhwinder Singh, Atul Arya, Sumeet Gupta
BACKGROUND: Diabetes is a downregulator of atrial natriuretic peptide (ANP), resulting in reduced nitric oxide level and low expression of endothelial nitric oxide synthase by which nitric oxide level get reduced. In the present study, we examined the role of ANP in reduced nitric oxide level, which may be responsible in controlling diabetic nephropathy in rats. METHODS: Serum nitrite/nitrate ratio, blood urea nitrogen, protein in urine, urinary output, serum creatinine, serum cholesterol, kidney weight, kidney hypertrophy, renal cortical collagen content, thiobarbituric acid level, and antioxidant enzymatic activities were assessed...
April 19, 2018: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29671019/lixisenatide-a-novel-glp-1-analog-protects-against-cerebral-ischemia-reperfusion-injury-in-diabetic-rats
#16
Rania G Abdel-Latif, Gehan H Heeba, Ashraf Taye, Mohamed M A Khalifa
Type 2 diabetes mellitus (T2DM) is a major risk factor for ischemic stroke accompanied by vascular dysfunction and poor cerebrovascular outcome. Lixisenatide is a glucagon like peptide-1 (GLP-1) analog that is recently used for T2DM treatment with established neuroprotective properties. This study investigated and compared the neuroprotective effect of lixisenatide against glimepiride on diabetic rats subjected to global cerebral ischemia/reperfusion (I/R) injury. T2DM-induced adult male Wistar rats were administered lixisenatide or glimepiride prior to induction of global cerebral I/R-induced injury...
April 18, 2018: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29669713/heme-oxygenase-1-in-macrophages-drives-septic-cardiac-dysfunction-via-suppressing-lysosomal-degradation-of-inducible-nitric-oxide-synthase
#17
Liangliang Jia, Yaping Wang, Yidong Wang, Yuankun Ma, Jian Shen, Zurong Fu, Yue Wu, Sheng'an Su, Yuhao Zhang, Zhejun Cai, Jian'an Wang, Meixiang Xiang
<u>Rationale:</u> To date, our understanding of the role of heme oxygenase-1 (HO-1) in inflammatory diseases has mostly been limited to its catalytic function and the potential for its heme-related catabolic products to suppress inflammation and oxidative stress. Whether and how HO-1 in macrophages plays a role in the development of septic cardiac dysfunction has never been explored. <u>Objective:</u> Here, we investigated the role of macrophage-derived HO-1 in septic cardiac dysfunction...
April 18, 2018: Circulation Research
https://www.readbyqxmd.com/read/29669031/auxin-acts-downstream-of-ethylene-and-nitric-oxide-to-regulate-magnesium-deficiency-induced-root-hair-development-in-arabidopsis-thaliana
#18
Miao Liu, Haihua Zhang, Xianzhi Fang, Yongsong Zhang, Chongwei Jin
This study examines the association of auxin with ethylene and nitric oxide (NO) in regulating the magnesium-deficiency-induced root hair development in Arabidopsis thaliana. With magnesium (Mg) deficiency, both ethylene and NO promoted the elevation of root auxin levels in roots by inducing the expression of AUX1, PIN1, and PIN2 transporters. In turn, auxin stimulated ethylene and NO production by activating the activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase (ACO), ACC synthase (ACS), nitrate reductase (NR), and NO synthase-like (NOS-L)...
April 16, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29668503/tetrahydroxystilbene-glycoside-antagonizes-%C3%AE-amyloid-induced-inflammatory-injury-in-microglia-cells-by-regulating-pu-1-expression
#19
Chenli Jiao, Feng Gao, Li Ou, Jinhua Yu, Min Li, Peifeng Wei, Feng Miu
Inhibiting β-amyloid (Aβ)-induced microglial activation is proposed as an effective strategy for the treatment of Alzheimer's disease. Tetrahydroxystilbene glycoside (TSG) is the main active ingredient of Polygonum multiflorum and has a wide range of biological properties, including antiinflammation. Here, we focused on the function and regulatory mechanism of TSG in Aβ-induced N9 and BV2 cells. The results showed that Aβ treatment induced the activation of microglia cells and the production of inflammatory molecules, including inducible nitric oxide synthase, nitric oxide, cyclooxygenase 2, and prostaglandin E2, which were significantly inhibited by TSG pretreatment...
April 17, 2018: Neuroreport
https://www.readbyqxmd.com/read/29668263/neuroprotective-effect-of-alpha-linolenic-acid-against-a%C3%AE-mediated-inflammatory-responses-in-c6-glial-cell
#20
Ah Young Lee, Myoung Hee Lee, Sanghyun Lee, Eun Ju Cho
Therapeutic approaches for neurodegeneration, such as Alzheimer's disease (AD), have been widely studied. One of the critical hallmarks of AD is accumulation of amyloid beta (Aβ). Aβ induces neurotoxicity and releases inflammatory mediators or cytokines through activation of glial cell, and these pathological features are observed in AD patient's brain. The purpose of this study is to investigate the protective effect of alpha-linolenic acid (ALA) on Aβ25-35 -induced neurotoxicity in C6 glial cells. Exposure of C6 glial cells to 50 μM 25-35 caused cell death, over-production of nitric oxide (NO), and pro-inflammatory cytokines release [interleukin (IL)-6 and tumor necrosis factor-α], while treatment of ALA increased cell viability and markedly attenuated Aβ25-35 -induced excessive production of NO and those inflammatory cytokines...
April 18, 2018: Journal of Agricultural and Food Chemistry
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