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nitric oxide synthase

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https://www.readbyqxmd.com/read/28088561/mutant-dd-genotype-of-nfkb1-gene-is-associated-with-the-susceptibility-and-severity-of-coronary-artery-disease
#1
Jun-Yi Luo, Xiao-Mei Li, Yun Zhou, Qiang Zhao, Bang-Dang Chen, Fen Liu, Xiao-Cui Chen, Hong Zheng, Yi-Tong Ma, Xiao-Ming Gao, Yi-Ning Yang
Nuclear factor κappa B (NF-κB) is an important transcription factor in the development and progression of coronary artery disease (CAD). Recent evidence suggests that -94 ATTG ins/del mutant in the promoter of NFKB1 gene is an essential functional mutant. The present study demonstrated the frequencies of the del/del (DD) genotype and del (D) allele were significantly higher in CAD patients than in controls. CAD patients carrying mutant DD genotype had worse stenosis of diseased coronary arteries compared to those carrying ins/ins (II) or ins/del (ID) genotype...
January 11, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28088559/increased-expression-of-carbon-monoxide-producing-enzymes-in-the-mpoa-after-sexual-experience-in-male-rats
#2
Christopher L Robison, Jenna A McHenry, Elaine M Hull
The hypothalamus contains numerous nuclei involved in the regulation of reproductive, stress, circadian, and homeostatic behaviors, with many of these nuclei concentrated within the preoptic and anterior regions. The gaseous neurotransmitter, nitric oxide (NO), has already been shown to have an important regulatory role within the medial preoptic area (MPOA) of the anterior hypothalamus, where it facilitates sexual behaviors. However, little is known about the role of other gaseous neurotransmitters in this area...
January 11, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28087909/-tripotolide-ameliorates-inflammation-and-apoptosis-induced-by-focal-cerebral-ischemia-reperfusion-in-rats
#3
Shi Bai, Yayi Sun, Lijuan Wu, Zhongmin Wu, Marong Fang
Objective: To investigate the effects of triptolide on inflammation and apoptosis induced by focal cerebral ischemia/reperfusion in rats. Methods: The rat model of focal cerebral ischemia/reperfusion injury was established according to Longa's method. A total of 80 SD rats were randomly divided into 5 groups:normal control, sham group, DMSO group, middle cerebral artery occlusion (MCAO) group, and MCAO with tripolide treatment group. TTC staining was used to examine the site and volume of cerebral infarction, and Longa score was employed for neurological disorders measurement...
May 25, 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/28087411/aspirin-prevents-tnf-%C3%AE-induced-endothelial-cell-dysfunction-by-regulating-the-nf-%C3%AE%C2%BAb-dependent-mir-155-enos-pathway-role-of-a-mir-155-enos-axis-in-preeclampsia
#4
Joohwan Kim, Kyu-Sun Lee, Ji-Hee Kim, Dong-Keon Lee, Minsik Park, Seunghwan Choi, Wonjin Park, Suji Kim, Yoon Kyoung Choi, Jong Yun Hwang, Jongseon Choe, Moo-Ho Won, Dooil Jeoung, Hansoo Lee, Sungwoo Ryoo, Kwon-Soo Ha, Young-Guen Kwon, Young-Myeong Kim
Preeclampsia is an inflammatory disease with endothelial cell dysfunction that occurs via decreased endothelial nitric oxide synthase/nitric oxide (eNOS/NO) activity. Aspirin reduces the incidence of hypertensive pregnancy complications. However, the underlying mechanism has not been clearly explained. Here, we found that tumor necrosis factor (TNF)-α, microRNA (miR)-155, and eNOS levels as well as endothelial redox phenotype were differentially regulated in preeclamptic patients, implying the involvement of TNF-α- and redox signal-mediated miR-155 biogenesis and eNOS downregulation in the pathogenesis of preeclampsia...
January 10, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28087254/anti-diabetic-drug-metformin-dilates-retinal-blood-vessels-through-activation-of-amp-activated-protein-kinase-in-rats
#5
Asami Mori, Eriko Ishikawa, Tomoyo Amano, Kenji Sakamoto, Tsutomu Nakahara
The aim of this study was to examine whether metformin, a biguanide anti-hyperglycemic drug, dilates retinal blood vessels in rats. Ocular fundus images were captured with an original high-resolution digital fundus camera in vivo and diameters of retinal blood vessels were measured. Both systemic blood pressure and heart rate were continuously recorded. Metformin (0.01-0.3mg/kg/min) increased diameters of retinal blood vessels in a dose-dependent manner. This retinal vasodilator effect of metformin was abolished by compound C, an inhibitor of AMP-activated protein kinase (AMPK), and N(G)-nitro-L-arginine methyl ester, an inhibitor of nitric oxide (NO) synthase...
January 10, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28086859/cheongsangbangpung-tang-ameliorated-the-acute-inflammatory-response-via-the-inhibition-of-nf-%C3%AE%C2%BAb-activation-and-mapk-phosphorylation
#6
Seon Young Kim, Sang Mi Park, Min Hwangbo, Jong Rok Lee, Sung Hui Byun, Sae Kwang Ku, Il Je Cho, Sang Chan Kim, Seon Young Jee, Sook Jahr Park
BACKGROUND: Cheongsangbangpung-tang (CBT) is a traditional herbal formula used in Eastern Asia to treat heat-related diseases and swellings in the skin. The present study was conducted to evaluate the anti-inflammatory effects of cheongsangbangpung-tang extract (CBTE) both in vitro and in vivo. METHODS: The in vitro effects of CBTE on the lipopolysaccharide (LPS)-induced production of inflammation-related proteins were examined in RAW 264.7 cells. The levels of nitric oxide (NO) were measured with the Griess reagent...
January 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28086773/pleurotus-giganteus-berk-karun-hyde-the-giant-oyster-mushroom-inhibits-no-production-in-lps-h2o2-stimulated-raw-264-7-cells-via-stat-3-and-cox-2-pathways
#7
Asweni Baskaran, Kek Heng Chua, Vikineswary Sabaratnam, Mani Ravishankar Ram, Umah Rani Kuppusamy
BACKGROUND: Pleurotus giganteus (Berk. Karunarathna and K.D. Hyde), has been used as a culinary mushroom and is known to have medicinal properties but its potential as an anti-inflammatory agent to mitigate inflammation triggered diseases is untapped. In this study, the molecular mechanism underlying the protective effect of ethanol extract of P. giganteus (EPG) against lipopolysaccharide (LPS) and combination of LPS and hydrogen peroxide (H2O2)-induced inflammation on RAW 264.7 macrophages was investigated...
January 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28080141/low-concentration-of-oleic-acid-exacerbates-lps-induced-cell-death-and-inflammation-in-human-alveolar-epithelial-cells
#8
Altug Kucukgul, Suat Erdogan
PURPOSE: The current study aimed to investigate in vitro effects of oleic acid on lipopolysaccharide (LPS)-induced acute lung injury in the human lung epithelial cells (A549). MATERIALS AND METHODS: The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests. Selected gene expression levels were analyzed by Real-Time Quantitative-Polymerase Chain Reaction (RT-qPCR). RESULTS: 24 hours of LPS (100 ng/mL) exposure decreased the cells viability by 44...
January 12, 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/28079268/renoprotective-effect-of-hypericum-perforatum-against-diabetic-nephropathy-in-rats-insights-in-the-underlying-mechanisms
#9
Dalia M Abd El Motteleb, Dalia I Abd El Aleem
Oxidative stress and inflammation play a key role in the initiation and progression of diabetic nephropathy (DN). The present study aimed to investigate the possible protective effect of hypericum perforatum (HP) against DN. Rats were allocated into six groups; control: received normal saline, diabetic untreated (DM): received single dose of streptozotocin (STZ) after injection of nicotinamide (NA), gliclazide: received STZ,NA+ gliclazide (10mg/kg), DM+HP50, DM+HP100, DM+HP200:received STZ,NA and HP 50, 100, 200 mg/kg respectively...
January 12, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28077855/the-nox2-derived-reactive-oxygen-species-damaged-endothelial-nitric-oxide-system-via-suppressed-bkca-skca-in-preeclampsia
#10
Jie Chen, Qinqin Gao, Lin Jiang, Xueqin Feng, Xiaolin Zhu, Xiaorong Fan, Caiping Mao, Zhice Xu
The endothelial nitric oxide (NO) system may be damaged in preeclampsia; however, the involved mechanisms are unclear. In this study, we used primary human umbilical vein endothelial cells (HUVECs) to evaluate the endothelial NO system in preeclampsia and to determine the underlying mechanisms that are involved. We isolated and cultured HUVECs from normal and preeclamptic pregnancies and evaluated endothelial NO synthase enzyme (eNOS) expression and NO production. Whole-cell K(+) currents and oxidative stress were also determined in normal and preeclamptic HUVECs...
January 12, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28077315/inhibition-of-inos-alleviates-cognitive-deficits-and-depression-in-diabetic-mice-through-downregulating-the-no-sgc-cgmp-pkg-signal-pathway
#11
Xiao Yan Zhou, Fang Zhang, Chang Jiang Ying, Jing Chen, Ling Chen, Jing Dong, Yue Shi, Mang Tang, Xiao Tong Hu, Zhi Hua Pan, Na Na Xu, Kui Yang Zheng, Ren Xian Tang, Yuan Jian Song
Diabetes mellitus often results in a number of complications involving impaired brain function, including cognitive deficits and depression. However, the potential mechanisms for diabetes-related cognitive deficits and depression are not fully understood. Neurons in the hippocampal, cortical and amygdala functional regions are more susceptible to damage during hyperglycemia. Neuroprotection in the brain can rescue cognitive deficits and depression induced by hyperglycemia. This study investigated the potential mechanisms underlying diabetes-related congnitive deficits and depression, determined whether the inflammatory factor inducible nitric oxide synthase (iNOS) and the nitric oxide (NO)/soluble guanylyl cyclases (sGC)/cyclic guanosine monophosphate (cGMP)/protein kinase G (PKG) pathway, play key roles in cognitive deficits and depression associated...
January 8, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28076881/constitutive-nos-uncoupling-and-nadph-oxidase-upregulation-in-the-penis-of-type-2-diabetic-men-with-erectile-dysfunction
#12
B Musicki, A L Burnett
Erectile dysfunction (ED) associated with type 2 diabetes mellitus (T2DM) involves dysfunctional nitric oxide (NO) signaling and increased oxidative stress in the penis. However, the mechanisms of endothelial NO synthase (eNOS) and neuronal NO synthase (nNOS) dysregulation, and the sources of oxidative stress, are not well defined, particularly at the human level. The objective of this study was to define whether uncoupled eNOS and nNOS, and NADPH oxidase upregulation, contribute to the pathogenesis of ED in T2DM men...
January 11, 2017: Andrology
https://www.readbyqxmd.com/read/28075010/stimulatory-actions-of-a-novel-thiourea-derivative-on-large-conductance-calcium-activated-potassium-channels
#13
Sheng-Nan Wu, Jyh-Haur Chern, Santai Shen, Hwei-Hisen Chen, Ying-Ting Hsu, Chih-Chin Lee, Ming-Huan Chan, Ming-Chi Lai, Feng-Shiun Shie
AIM: In this study, we examine whether an anti-inflammatory thiourea derivative, compound #326, actions on ion channels. METHODS: The effects of compound #326 on Ca(2+) -activated K(+) channels were evaluated by patch-clamp recordings obtained in cell-attached, inside-out or whole-cell configuration. RESULTS: In pituitary GH3 cells, compound #326 increased the amplitude of Ca(2+) -activated K(+) currents (IK(Ca) ) with an EC50 value of 11...
January 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28074952/upregulation-of-caveolin-1-by-mulberry-leaf-extract-and-its-major-components-chlorogenic-acid-derivatives-attenuates-alcoholic-steatohepatitis-via-inhibition-of-oxidative-stress
#14
Yi-Ju Lee, Jeng-Dong Hsu, Wea-Lung Lin, Shao-Hsuan Kao, Chau-Jong Wang
Excessive alcohol uptake exerts hepatocellular toxicity, ultimately leading to multiple liver diseases such as steatohepatitis and liver cirrhosis. Here, we aimed to explore the effects of mulberry leaf extract (MLE) and its major components chlorogenic acid (CGA) and neochlorogenic acid (nCGA) on alcoholic steatohepatitis. We determined the composition of MLE using liquid chromatography-mass spectrometric (LC-MS) analysis, which showed that MLE consisted of mainly chlorogenic acid derivatives and other polyphenols...
January 11, 2017: Food & Function
https://www.readbyqxmd.com/read/28074650/revisiting-the-val-ile-mutation-in-mammalian-and-bacterial-no-synthases-a-spectroscopic-and-kinetic-study
#15
Marine Weisslocker-Schaetzel, Mehdi Lembrouk, Jerome Santolini, Pierre Dorlet
Nitric oxide (NO) is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a biopterin cofactor. Through genome sequencing, proteins with high homology to the oxygenase domain of NOSs have been identified, in particular in bacteria. The active site is highly conserved except for a valine residue in the distal pocket that is replaced by an isoleucine in bacteria. This switch was previously reported to influence the kinetics of the reaction. We have used the V346I mutant of the mouse inducible NOS (iNOS) as well as the I224V mutant of the NOS from Bacillus subtilis (bsNOS) to study their spectroscopic signatures in solution and look for potential structural differences compared to their respective wild types...
January 11, 2017: Biochemistry
https://www.readbyqxmd.com/read/28074232/vasoactive-actions-of-nitroxyl-hno-are-preserved-in-resistance-arteries-in-diabetes
#16
Marianne Tare, Rushita S R Kalidindi, Kristen J Bubb, Helena C Parkington, Wee-Ming Boon, Xiang Li, Christopher G Sobey, Grant R Drummond, Rebecca H Ritchie, Barbara K Kemp-Harper
Endothelial dysfunction is a major risk factor for the vascular complications of diabetes. Increased reactive oxygen species (ROS) generation, a hallmark of diabetes, reduces the bioavailability of endothelial vasodilators, including nitric oxide (NO·). The vascular endothelium also produces the one electron reduced and protonated form of NO·, nitroxyl (HNO). Unlike NO·, HNO is resistant to scavenging by superoxide anions (·O2(─)). The fate of HNO in resistance arteries in diabetes is unknown. We tested the hypothesis that the vasodilator actions of endogenous and exogenous HNO are preserved in resistance arteries in diabetes...
January 10, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28071183/5-aminosalicylic-acid-inhibits-inflammatory-responses-by-suppressing-jnk-and-p38-activity-in-murine-macrophages
#17
Tingli Qu, Erbing Wang, Baofen Jin, Weiping Li, Ruiling Liu, Zheng-Bao Zhao
CONTEXT: 5-Aminosalicylic acid (5-ASA), as an anti-inflammatory drug, has been extensively used for the treatment of mild to moderate active ulcerative colitis (UC), but the possible mechanisms of action remain unclear. OBJECTIVE: To investigate the effects of 5-ASA on the production of inflammatory mediators by murine macrophages stimulated with lipopolysaccharide (LPS), and determine the underlying pharmacological mechanism of action. MATERIALS AND METHODS: The levels of nitric oxide (NO) and interleukin-6 (IL-6) were measured by Varioskan Flash and IL-6 Enzyme-Linked Immunosorbent Assay sets...
February 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/28070931/anti-rheumatoid-arthritis-effect-of-kaejadan-via-analgesic-and-antiinflammatory-activity-in-vivo-and-in-vitro
#18
Jung Jae Yoon, Eun Jung Sohn, Jung Hyo Kim, Jai Wha Seo, Sung-Hoon Kim
Although Kaejadan (KJD), an herbal cocktail of three medicinal plants (Lithospermum erythrorhizon, Cinnamomum loureirii, and Salvia miltiorrhiza), has been traditionally used for the treatment of rheumatoid arthritis, its scientific evidence is not fully understood. Hence, we investigated antiinflammatory and analgesic mechanism of KJD in vivo and in vitro. Kaejadan suppressed the number of writhing responses in mice treated by acetic acid and showed antinociceptive effect by tail-flick test. Kaejadan abrogated serotonin or carrageenan or Freund's complete adjuvant (FCA)-induced paw edema and also reduced the level of Evans Blue for vascular permeability...
January 9, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28070318/piceatannol-exhibits-anti-inflammatory-effects-on-macrophages-interacting-with-adipocytes
#19
Takayuki Yamamoto, Yongjia Li, Yuki Hanafusa, Yu-Sheng Yeh, Hiroko Maruki-Uchida, Shinpei Kawakami, Masahiko Sai, Tsuyoshi Goto, Tatsuhiko Ito, Teruo Kawada
Piceatannol (PIC), a natural analog of resveratrol (RES), is a phytochemical found in passion fruit seeds. To clarify the effects of PIC on obesity-induced inflammation in adipose tissue, we investigated the anti-inflammatory activity of PIC-related compounds (PIC, RES, and metabolites from PIC) in culture models of obese adipose tissue. Lipopolysaccharide (LPS) and conditioned medium from 3T3-L1 adipocytes (3T3-L1-CM) enhanced proinflammatory gene expression and synthesis of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in RAW264...
January 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28070018/curcumin-supplementation-improves-vascular-endothelial-function-in-healthy-middle-aged-and-older-adults-by-increasing-nitric-oxide-bioavailability-and-reducing-oxidative-stress
#20
Jessica R Santos-Parker, Talia R Strahler, Candace J Bassett, Nina Z Bispham, Michel B Chonchol, Douglas R Seals
We hypothesized that curcumin would improve resistance and conduit artery endothelial function and large elastic artery stiffness in healthy middle-aged and older adults. Thirty-nine healthy men and postmenopausal women (45-74 yrs) were randomized to 12 weeks of curcumin (2000 mg/day Longvida®; n=20) or placebo (n=19) supplementation. Forearm blood flow response to acetylcholine infusions (FBFACh; resistance artery endothelial function) increased 37% following curcumin supplementation (107±13 vs. 84±11 AUC at baseline, P=0...
January 3, 2017: Aging
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