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https://www.readbyqxmd.com/read/28707625/bioprinting-of-functional-vascularized-mouse-thyroid-gland-construct
#1
Elena A Bulanova, Elizaveta V Koudan, Jonathan Degosserie, Charlotte Heymans, Frederico DAS Pereira, Vladislav A Parfenov, Yi Sun, Qi Wang, Suraya A Akhmedova, Irina K Sviridova, Natalia S Sergeeva, Georgy A Frank, Yusef D Khesuani, Christophe E Pierreux, Vladimir A Mironov
Bioprinting can be defined as additive biofabrication of 3D tissues and organ constructs using tissue spheroids, capable of self-assembly, as building blocks. Thyroid gland, a relatively simple endocrine organ, is suitable for testing the proposed bioprinting technology. Here we report the bioprinting of functional vascularized mouse thyroid gland construct from embryonic tissue spheroids as a proof of concept. Based on the self-assembly principle, we generated thyroid tissue starting from thyroid spheroids (TS) and allantoic spheroids (AS), as a source of thyrocytes and endothelial cells (EC), respectively...
July 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28704481/renal-auto-transplantation-promotes-cortical-microvascular-network-remodeling-in-a-preclinical-porcine-model
#2
Souleymane Maïga, Geraldine Allain, Thierry Hauet, Jerome Roumy, Edouard Baulier, Michel Scepi, Manuel Dierick, Luc Van Hoorebeke, Patrick Hannaert, Franck Guy, Frederic Favreau
The vascular network is a major target of ischemia-reperfusion, but has been poorly investigated in renal transplantation. The aim of this study was to characterize the remodeling of the renal vascular network that follows ischemia-reperfusion along with the most highly affected cortex section in a preclinical renal transplantation model. There were two experimental groups. The first was a grafted kidney group consisting of large white pigs for which the left kidney was harvested, cold flushed, preserved for 24 h in the University of Wisconsin's preservation solution, and then auto-transplanted (n = 5); the right kidney was removed to mimic the situation of human kidney transplantation...
2017: PloS One
https://www.readbyqxmd.com/read/28686597/altered-paracellular-cation-permeability-due-to-a-rare-cldn10b-variant-causes-anhidrosis-and-kidney-damage
#3
Joakim Klar, Jörg Piontek, Susanne Milatz, Muhammad Tariq, Muhammad Jameel, Tilman Breiderhoff, Jens Schuster, Ambrin Fatima, Maria Asif, Muhammad Sher, Katrin Mäbert, Anja Fromm, Shahid M Baig, Dorothee Günzel, Niklas Dahl
Claudins constitute the major component of tight junctions and regulate paracellular permeability of epithelia. Claudin-10 occurs in two major isoforms that form paracellular channels with ion selectivity. We report on two families segregating an autosomal recessive disorder characterized by generalized anhidrosis, severe heat intolerance and mild kidney failure. All affected individuals carry a rare homozygous missense mutation c.144C>G, p.(N48K) specific for the claudin-10b isoform. Immunostaining of sweat glands from patients suggested that the disease is associated with reduced levels of claudin-10b in the plasma membranes and in canaliculi of the secretory portion...
July 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28656614/automatic-renal-segmentation-for-mr-urography-using-3d-grabcut-and-random-forests
#4
Umit Yoruk, Brian A Hargreaves, Shreyas S Vasanawala
PURPOSE: To introduce and evaluate a fully automated renal segmentation technique for glomerular filtration rate (GFR) assessment in children. METHODS: An image segmentation method based on iterative graph cuts (GrabCut) was modified to work on time-resolved 3D dynamic contrast-enhanced MRI data sets. A random forest classifier was trained to further segment the renal tissue into cortex, medulla, and the collecting system. The algorithm was tested on 26 subjects and the segmentation results were compared to the manually drawn segmentation maps using the F1-score metric...
June 27, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28644734/high-throughput-phenotypic-screening-of-kinase-inhibitors-to-identify-drug-targets-for-polycystic-kidney-disease
#5
Tijmen H Booij, Hester Bange, Wouter N Leonhard, Kuan Yan, Michiel Fokkelman, Steven J Kunnen, Johannes G Dauwerse, Yu Qin, Bob van de Water, Gerard J P van Westen, Dorien J M Peters, Leo S Price
Polycystic kidney disease (PKD) is a prevalent disorder characterized by renal cysts that lead to kidney failure. Various signaling pathways have been targeted to stop disease progression, but most interventions still focus on alleviating PKD-associated symptoms. The mechanistic complexity of the disease, as well as the lack of functional in vitro assays for compound testing, has made drug discovery for PKD challenging. To identify modulators of PKD, Pkd1(-/-) kidney tubule epithelial cells were applied to a scalable and automated 3D cyst culture model for compound screening, followed by phenotypic profiling to determine compound efficacy...
June 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28643936/phenotypic-analysis-of-organoids-by-proteomics
#6
REVIEW
Alexis Gonneaud, Claude Asselin, François Boudreau, François-Michel Boisvert
The development of 3D cell cultures into self-organizing organ-like structures named organoids provides a model that better reflects in vivo organ physiology and their functional properties. Organoids have been established from several organs, such as the intestine, prostate, brain, liver, kidney and pancreas. With recent advances in high-throughput and -omics profiling technologies, it is now possible to study the mechanisms of cellular organisation at the systems level. It is therefore not surprising that these methods are now used to characterize organoids at the transcriptomic, proteomic, chromatin state and transcription factor DNA-binding levels...
June 23, 2017: Proteomics
https://www.readbyqxmd.com/read/28632265/3d-printed-conformal-microfluidics-for-isolation-and-profiling-of-biomarkers-from-whole-organs
#7
Manjot Singh, Yuxin Tong, Kelly Webster, Ellen Cesewski, Alexander P Haring, Sahil Laheri, Bill Carswell, Timothy J O'Brien, Charles H Aardema, Ryan S Senger, John L Robertson, Blake N Johnson
The ability to interface microfluidic devices with native complex biological architectures, such as whole organs, has the potential to shift the paradigm for the study and analysis of biological tissue. Here, we show 3D printing can be used to fabricate bio-inspired conformal microfluidic devices that directly interface with the surface of whole organs. Structured-light scanning techniques enabled the 3D topographical matching of microfluidic device geometry to porcine kidney anatomy. Our studies show molecular species are spontaneously transferred from the organ cortex to the conformal microfluidic device in the presence of fluid flow through the organ-conforming microchannel...
June 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28615647/differential-synchrotron-x-ray-imaging-markers-based-on-the-renal-microvasculature-for-tubulointerstitial-lesions-and-glomerulopathy
#8
Yu-Chuan Lin, Yeukuang Hwu, Guo-Shu Huang, Michael Hsiao, Tsung-Tse Lee, Shun-Min Yang, Ting-Kuo Lee, Nan-Yow Chen, Sung-Sen Yang, Ann Chen, Shuk-Man Ka
High resolution synchrotron microtomography capable of revealing microvessels in three dimensional (3D) establishes distinct imaging markers of mouse kidney disease strongly associated to renal tubulointerstitial (TI) lesions and glomerulopathy. Two complementary mouse models of chronic kidney disease (CKD), unilateral ureteral obstruction (UUO) and focal segmental glomerulosclerosis (FSGS), were used and five candidates of unique 3D imaging markers were identified. Our characterization to differentially reflect the altered microvasculature of renal TI lesions and/or glomerulopathy demonstrated these image features can be used to differentiate the disease status and the possible cause therefore qualified as image markers...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28599598/uniform-neural-tissue-models-produced-on-synthetic-hydrogels-using-standard-culture-techniques
#9
Christopher Barry, Matthew T Schmitz, Nicholas E Propson, Zhonggang Hou, Jue Zhang, Bao K Nguyen, Jennifer M Bolin, Peng Jiang, Brian E McIntosh, Mitchell D Probasco, Scott Swanson, Ron Stewart, James A Thomson, Michael P Schwartz, William L Murphy
The aim of the present study was to test sample reproducibility for model neural tissues formed on synthetic hydrogels. Human embryonic stem (ES) cell-derived precursor cells were cultured on synthetic poly(ethylene glycol) (PEG) hydrogels to promote differentiation and self-organization into model neural tissue constructs. Neural progenitor, vascular, and microglial precursor cells were combined on PEG hydrogels to mimic developmental timing, which produced multicomponent neural constructs with 3D neuronal and glial organization, organized vascular networks, and microglia with ramified morphologies...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28593430/the-prevalence-and-significance-of-renal-perfusion-defects-in-early-kidney-transplants-quantified-using-3d-contrast-enhanced-ultrasound-ceus
#10
Ben Stenberg, M Wilkinson, S Elliott, N Caplan
OBJECTIVES: Vascular complications are one of the most common causes of early kidney transplant dysfunction. Contrast enhanced ultrasound increases sensitivity to vascular changes. The aim of this study was to assess the prevalence and size of vascular abnormalities in early renal transplants using 3D CEUS and the significance of perfusion defects on renal function. METHODS: Ninety-nine renal transplant patients underwent 3D CEUS after surgery to quantify perfusion defects as percentage total renal volume (TRV)...
June 7, 2017: European Radiology
https://www.readbyqxmd.com/read/28587402/establishment-and-evaluation-of-a-reversible-two-kidney-one-clip-renovascular-hypertensive-rat-model
#11
Li-Qiang Li, Jian Zhang, Rong Wang, Jian-Xin Li, Yong-Quan Gu
The aim of the present study was to establish and evaluate a novel and reversible two-kidney, one-clip renovascular hypertensive rat model with a titanium vascular clip. A total of 40 male Sprague-Dawley rats were evenly and randomly divided into a sham-operated group, and 3, 7, 12 and 28D groups (namely removing the vascular clip in the renovascular hypertensive model after 3, 7, 12 and 28 days, respectively). The systolic blood pressure (SBP) and plasma renin activity (PRA) were measured, and color duplex imaging was conducted before placing the clips, as well as before and after removing them...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28576040/engineered-3d-scaffolds-of-photocrosslinked-chitosan-gelatin-hydrogel-hybrids-for-chronic-wound-dressings-and-regeneration
#12
Isadora C Carvalho, Herman S Mansur
Wound repair is one of the most complex biological processes in human life. To date, no ideal biomaterial solution has been identified, which that encompasses all functions and properties of real skin tissue. Thus, this study focused on the synthesis of new biocompatible hybrid hydrogel scaffolds based on methacrylate-functionalized high molecular mass chitosan with gelatin-A photocrosslinked with UV radiation to tailor matrix network properties. These hybrid hydrogels were produced via freeze-drying and were extensively characterized by swelling and degradation measurements, Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM-EDS), and micro-computed tomography (micro-CT)...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28539333/endothelial-markers-expressing-stromal-cells-are-critical-for-kidney-formation
#13
Elina Mukherjee, Katherine Virginia Maringer, Emily Papke, Daniel S Bushnell, Caitlin M Schaefer, Rafael Kramann, Jacqueline Ho, Benjamin D Humphreys, Carlton M Bates, Sunder Sims-Lucas
Kidneys are highly vascularized and contain many distinct vascular beds. However, the origins and roles of developing renal endothelial cells in the formation of the kidney are unclear. We have shown that the Foxd1-positive renal stroma gives rise to endothelial marker expressing progenitors that are incorporated within a subset of peritubular capillaries; however, the significance of these cells is unclear. The purpose of this study was to determine whether deletion of Flk1 in the Foxd1 stroma was important for renal development...
May 24, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28534535/the-potential-of-organoids-in-urological-cancer-research
#14
REVIEW
Shangqian Wang, Dong Gao, Yu Chen
Technical advances in the development of organoid systems enable cell lines, primary adult cells, or stem or progenitor cells to develop into diverse, multicellular entities, which can self-renew, self-organize, and differentiate. These 3D organoid cultures have proven to be of value in increasing our understanding of the biology of disease and offer the potential of regenerative and genetic therapies. The successful application of 3D organoids derived from adult tissue into urological cancer research can further our understanding of these diseases and could also provide preclinical cancer models to realize the precision medicine paradigm by therapeutic screening of individual patient samples ex vivo...
July 2017: Nature Reviews. Urology
https://www.readbyqxmd.com/read/28520334/kidney-anatomy-three-dimensional-3d-printed-pelvicalyceal-system-models-of-the-collector-system-improve-the-diagnosis-and-treatment-of-stone-disease
#15
EDITORIAL
Luciano A Favorito
No abstract text is available yet for this article.
May 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28515297/attenuation-of-foot-and-mouth-disease-virus-by-engineering-viral-polymerase-fidelity
#16
Devendra K Rai, Fayna Diaz-San Segundo, Grace Campagnola, Anna Keith, Elizabeth A Schafer, Anna Kloc, Teresa de Los Santos, Olve Peersen, Elizabeth Rieder
Foot-and-mouth disease virus (FMDV) RNA-dependent RNA polymerase (RdRp or 3D(pol)) catalyzes viral RNA synthesis. Its characteristic low fidelity and absence of proofreading activity allows FMDV to rapidly mutate and adapt to dynamic environments. In this study, we used the structure of FMDV 3D(pol) in combination with prior results from similar picornaviral polymerases to design point mutations that would alter replication fidelity. In particular, we targeted Trp237 within conserved polymerase motif A because of the low reversion potential inherent in the single UGG codon...
May 17, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28498800/locally-applied-5-fluorouracil-loaded-slow-release-patch-prevents-pancreatic-cancer-growth-in-an-orthotopic-mouse-model
#17
In Kyong Shim, Hye-Jin Yi, Hee-Gyeong Yi, Chan Mi Lee, Yu Na Lee, Yeong-Jin Choi, Seong-Yun Jeong, Eunsung Jun, Robert M Hoffman, Dong-Woo Cho, Song Cheol Kim
To obtain improved efficacy against pancreatic cancer, we investigated the efficacy and safety of a locally-applied 5-fluorouracil (5-FU)-loaded polymeric patch on pancreatic tumors in an orthotopic nude-mouse model. The 5-FU-releasing polymeric patch was produced by 3D printing. After application of the patch, it released the drug slowly for 4 weeks, and suppressed BxPC-3 pancreas cancer growth. Luciferase imaging of BxPC3-Luc cells implanted in the pancreas was performed longitudinally. The drug patch delivered a 30...
June 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28497498/cryoprotection-and-banking-of-living-cells-in-a-3d-multiple-emulsion-based-carrier
#18
Ewa Dluska, Zhanfeng Cui, Agnieszka Markowska-Radomska, Agata Metera, Konrad Kosicki
This paper addresses the problem of the low viability of cells during traditional cryopreservation by developing multiple emulsion-based carriers for the encapsulation and cryopreservation of cells. Multiple emulsions are hierarchically structured dispersed systems of "droplets in drops", which allow bioactive ingredients to be encapsulated. The problem of low survival rates refers to the stage of their freezing-thawing in traditional suspensions. During freezing-thawing processes, irreversible damage to cells occurs due to the formation of ice crystals, cell dehydration, and cryoprotectants' toxicity...
May 12, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28484293/advantages-of-t2-weighted-three-dimensional-and-t1-weighted-three-dimensional-contrast-medium-enhanced-magnetic-resonance-urography-in-examination-of-the-child-population
#19
Adnan Sehic, Fuad Julardzija, Sandra Vegar-Zubovic, Irmina Sefic-Pasic
AIM: The aim of this study is to prove the advantages of combined use of T2 weighted three dimensional (T2 W 3D) and T1 weighted three dimensional contrast medium enhanced (T1 W 3D CE) magnetic resonance (MR) urography in displaying urinary tract in child population. MATERIAL AND METHODS: Total of 120 patients were included in the study, 71 (59%) male patients and 49 (41%) female patients. The study was conducted on the Radiology clinic, University of Sarajevo Clinical Center, during the period from February to November 2016...
March 2017: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/28478915/indentation-of-heterogeneous-soft-tissue-local-constitutive-parameter-mapping-using-an-inverse-method-and-an-automated-rig
#20
B Pierrat, D B MacManus, J G Murphy, M D Gilchrist
In the domain of soft tissue biomechanics, the development of numerical simulations has raised the experimental challenge of identifying local internal mechanical constitutive data of heterogeneous organs (e.g. brain tissue). In this context, this paper presents an ex-vivo alternative characterization method to full-field imaging techniques. It is based on automated, multiple indentations of an organ section using a custom-built rig, effectively allowing to map the viscoelastic and hyperelastic constitutive parameters of the tissue at the millimetre scale, under dynamic conditions...
April 2, 2017: Journal of the Mechanical Behavior of Biomedical Materials
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