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https://www.readbyqxmd.com/read/28520334/kidney-anatomy-three-dimensional-3d-printed-pelvicalyceal-system-models-of-the-collector-system-improve-the-diagnosis-and-treatment-of-stone-disease
#1
EDITORIAL
Luciano A Favorito
No abstract text is available yet for this article.
May 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28515297/attenuation-of-foot-and-mouth-disease-virus-by-engineering-viral-polymerase-fidelity
#2
Devendra K Rai, Fayna Diaz-San Segundo, Grace Campagnola, Anna Keith, Elizabeth A Schafer, Anna Kloc, Teresa de Los Santos, Olve Peersen, Elizabeth Rieder
Foot-and-mouth disease virus (FMDV) RNA-dependent RNA polymerase (RdRp or 3D(pol)) catalyzes viral RNA synthesis. Its characteristic low fidelity and absence of proofreading activity allows FMDV to rapidly mutate and adapt to dynamic environments. In this study, we used the structure of FMDV 3D(pol) in combination with prior results from similar picornaviral polymerases to design point mutations that would alter replication fidelity. In particular, we targeted Trp237 within conserved polymerase motif A because of the low reversion potential inherent in the single UGG codon...
May 17, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28498800/locally-applied-5-fluorouracil-loaded-slow-release-patch-prevents-pancreatic-cancer-growth-in-an-orthotopic-mouse-model
#3
In Kyong Shim, Hye-Jin Yi, Hee-Gyeong Yi, Chan Mi Lee, Yu Na Lee, Yeong-Jin Choi, Seong-Yun Jeong, Eunsung Jun, Robert M Hoffman, Dong-Woo Cho, Song Cheol Kim
To obtain improved efficacy against pancreatic cancer, we investigated the efficacy and safety of a locally-applied 5-fluorouracil (5-FU)-loaded polymeric patch on pancreatic tumors in an orthotopic nude-mouse model. The 5-FU-releasing polymeric patch was produced by 3D printing. After application of the patch, it released the drug slowly for 4 weeks, and suppressed BxPC-3 pancreas cancer growth. Luciferase imaging of BxPC3-Luc cells implanted in the pancreas was performed longitudinally. The drug patch delivered a 30...
April 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28497498/cryoprotection-and-banking-of-living-cells-in-a-3d-multiple-emulsion-based-carrier
#4
Ewa Dluska, Zhanfeng Cui, Agnieszka Markowska-Radomska, Agata Metera, Konrad Kosicki
This paper addresses the problem of the low viability of cells during traditional cryopreservation by developing multiple emulsion-based carriers for the encapsulation and cryopreservation of cells. Multiple emulsions are hierarchically structured dispersed systems of "droplets in drops", which allow bioactive ingredients to be encapsulated. The problem of low survival rates refers to the stage of their freezing-thawing in traditional suspensions. During freezing-thawing processes, irreversible damage to cells occurs due to the formation of ice crystals, cell dehydration, and cryoprotectants' toxicity...
May 12, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28484293/advantages-of-t2-weighted-three-dimensional-and-t1-weighted-three-dimensional-contrast-medium-enhanced-magnetic-resonance-urography-in-examination-of-the-child-population
#5
Adnan Sehic, Fuad Julardzija, Sandra Vegar-Zubovic, Irmina Sefic-Pasic
AIM: The aim of this study is to prove the advantages of combined use of T2 weighted three dimensional (T2 W 3D) and T1 weighted three dimensional contrast medium enhanced (T1 W 3D CE) magnetic resonance (MR) urography in displaying urinary tract in child population. MATERIAL AND METHODS: Total of 120 patients were included in the study, 71 (59%) male patients and 49 (41%) female patients. The study was conducted on the Radiology clinic, University of Sarajevo Clinical Center, during the period from February to November 2016...
March 2017: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/28478915/indentation-of-heterogeneous-soft-tissue-local-constitutive-parameter-mapping-using-an-inverse-method-and-an-automated-rig
#6
B Pierrat, D B MacManus, J G Murphy, M D Gilchrist
In the domain of soft tissue biomechanics, the development of numerical simulations has raised the experimental challenge of identifying local internal mechanical constitutive data of heterogeneous organs (e.g. brain tissue). In this context, this paper presents an ex-vivo alternative characterization method to full-field imaging techniques. It is based on automated, multiple indentations of an organ section using a custom-built rig, effectively allowing to map the viscoelastic and hyperelastic constitutive parameters of the tissue at the millimetre scale, under dynamic conditions...
April 2, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28471202/-use-of-porous-hydrogel-as-a-3d-scaffold-for-the-growth-of-leukemic-b-lymphocytes
#7
R Studená, D Horák, J Baloun, Z Plichta, Š Pospíšilová
BACKGROUND: Primary human B cells chronic lymphocytic leukemia undergoes apoptosis, from which they can be rescued by contact with stromal cells or by the addition of specific soluble factor, when cultured in vitro. For research purposes of the behavior of CLL cells we created 3D in vitro model in which we simulated appropriate microenvironment for CLL cells to allow study the mechanism of survival of these cells in long-term cultivation. MATERIAL AND METHODS: Our aim was the scaffold structure to be geometrically similar to the 3D morphology of supporting bone marrow tissue in a trabecular bone; the 3D scaffold was also designed to conform to biocompatibility, sufficiently large surface area for cell attachment, high porosity for cell migration, proliferation and transport of nutrients...
2017: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
https://www.readbyqxmd.com/read/28467997/treating-hepatitis-c-in-patients-with-renal-failure
#8
Sabela Lens, Sergio Rodriguez-Tajes, Laura-Patricia Llovet, Francisco Maduell, Maria-Carlota Londoño
BACKGROUND: There is a strong relationship between hepatitis C virus (HCV) infection and the kidney. Approximately 10-16% of the patients with HCV infection develop renal disease, and the prevalence of HCV infection in patients with renal dysfunction is higher than that of the general population (9.5 vs. 1.6%). Moreover, HCV-positive patients on hemodialysis (HD) have higher mortality rates as compared to HCV-negative patients also on HD, not only due to liver-related complications but also owing to cardiovascular disease...
2017: Digestive Diseases
https://www.readbyqxmd.com/read/28447987/live-cell-imaging-and-3d-analysis-of-angiotensin-receptor-type-1a-trafficking-in-transfected-human-embryonic-kidney-cells-using-confocal-microscopy
#9
Parnika Kadam, Ryan McAllister, Jeffrey S Urbach, Kathryn Sandberg, Susette C Mueller
Live-cell imaging is used to simultaneously capture time-lapse images of angiotensin type 1a receptors (AT1aR) and intracellular compartments in transfected human embryonic kidney-293 (HEK) cells following stimulation with angiotensin II (Ang II). HEK cells are transiently transfected with plasmid DNA containing AT1aR tagged with enhanced green fluorescent protein (EGFP). Lysosomes are identified with a red fluorescent dye. Live-cell images are captured on a laser scanning confocal microscope after Ang II stimulation and analyzed by software in three dimensions (3D, voxels) over time...
March 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447108/split-bolus-intravenous-contrast-material-injection-vs-single-bolus-injection-in-patients-following-endovascular-abdominal-aortic-repair-evar
#10
Johannes Boos, Vassilios Raptopoulos, Alexander Brook, Olga R Brook
PURPOSE: To investigate the feasibility and accuracy of a split-bolus contrast injection CTA (SB-CTA) compared to a single-bolus injection CTA (SI-CTA) protocol of post-EVAR patients. MATERIALS AND METHODS: In this IRB-approved study, patients who underwent SB-CTA and SI-CTA after EVAR were evaluated. Attenuation and contrast-to-noise ratio (CNR) of vessels and parenchymal organs were assessed. Subjective enhancement of vessels, quality of 3D reconstructions, and enhancement phase of parenchymal organs were assessed by two independent readers...
April 26, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28442416/molecular-characterization-and-expression-of-interleukin-10-and-interleukin-22-in-golden-pompano-trachinotus-ovatus-in-response-to-streptococcus-agalactiae-stimulus
#11
Yinhui Peng, Xiaohui Cai, Guoyin Zhang, Junlin Wang, Yuan Li, Zhiwen Wang, Bei Wang, Xiangying Xiong, Zaohe Wu, Jichang Jian
In the present study, members of the interleukin (IL)-10 family of cytokines, including IL-10 (TOIL-10) and IL-22 (TOIL-22) of golden pompano (Trachinotus ovatus), were cloned for the first time, and their expression patterns and 3D structures analyzed. The full-length cDNA sequences of TOIL-10 and TOIL-22 contained open reading frames of 564 and 567 bp, respectively. TOIL-10 and TOIL-22 shared higher homology (78%-89%) with the corresponding genes from various fish relative to other species (25%-34%) and contained the IL-10 family signature and four cysteine residues that are well conserved in other vertebrate IL-10 members...
April 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28430635/unsuccessful-mitosis-in-multicellular-tumour-spheroids
#12
Annie Molla, Morgane Couvet, Jean-Luc Coll
Multicellular spheroids are very attractive models in oncology because they mimic the 3D organization of the tumour cells with their microenvironment. We show here using 3 different cell types (mammary TSA/pc, embryonic kidney Hek293 and cervical cancer HeLa), that when the cells are growing as spheroids the frequency of binucleated cells is augmented as occurs in some human tumours.We therefore describe mitosis in multicellular spheroids by following mitotic markers and by time-lapse experiments. Chromosomes alignment appears to be correct on the metaphasic plate and the passenger complex is well localized on centromere...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28429072/kidney-development-and-perspectives-for-organ-engineering
#13
REVIEW
Ganna Reint, Aleksandra Rak-Raszewska, Seppo J Vainio
Organ transplantation is currently the best strategy for treating end stage renal disease (ESRD) but the numbers of donor kidneys available are not sufficient to meet the needs of the ever-increasing ESRD population. Therefore, developments in the field of tissue engineering are necessary to provide alternative treatments. Decellularization and three-dimensional (3D) bioprinting strategies may serve as attractive novel options. Since successful tissue engineering requires an in -depth understanding of organ development and regulatory pathways, we discuss signaling in renal development and the composition of the renal extracellular matrix before presenting progress in the decellularization and 3D bioprinting fields...
April 20, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28422807/clinical-robustness-of-accelerated-and-optimized-abdominal-diffusion-weighted-imaging
#14
Jana Taron, Jakob Weiß, Petros Martirosian, Ferdinand Seith, Alto Stemmer, Fabian Bamberg, Mike Notohamiprodjo
OBJECTIVES: The aim of this study was to assess the robustness of an accelerated and optimized diffusion-weighted sequence in clinical routine abdominal imaging using the simultaneous multislice (SMS) technique for scan time reduction and 3-dimensional (3D) diagonal diffusion mode to optimize image quality. MATERIALS AND METHODS: One hundred fifty consecutive patients received clinically indicated magnetic resonance imaging for abdominal imaging including an optimized SMS diffusion-weighted sequence (DWIOPT: diffusion mode 3D diagonal; SMS factor 2; scan time 1:44 minutes)...
April 19, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28418422/a-disease-model-of-diabetic-nephropathy-in-a-glomerulus-on-a-chip-microdevice
#15
Li Wang, Tingting Tao, Wentao Su, Hao Yu, Yue Yu, Jianhua Qin
Diabetic nephropathy is a major chronic renal complication of diabetes mellitus, and is the leading cause of end-stage kidney diseases. Establishing a disease model of diabetic nephropathy in vitro can accelerate the understanding of its mechanisms and pharmaceutical development. We provide the proof-of-principle for using a glomerulus-on-a-chip microdevice that reconstitutes organ-level kidney functions to create a human disease model of early stage diabetic nephropathy on chip. The microfluidic device, which recapitulates the glomerular microenvironment, consists of parallel channels lined by isolated primary glomerular microtissues that experience fluid flow to mimic the glomerular filtration barrier (GFB), including glomerular endothelial cells, 3D basement membrane and podocytes...
April 18, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28416944/evaluation-of-digital-pcr-as-a-technique-for-monitoring-acute-rejection-in-kidney-transplantation
#16
Hyeseon Lee, Young-Mi Park, Yu-Mee We, Duck Jong Han, Jung-Woo Seo, Haena Moon, Yu-Ho Lee, Yang-Gyun Kim, Ju-Young Moon, Sang-Ho Lee, Jong-Keuk Lee
Early detection and proper management of kidney rejection are crucial for the long-term health of a transplant recipient. Recipients are normally monitored by serum creatinine measurement and sometimes with graft biopsies. Donor-derived cell-free deoxyribonucleic acid (cfDNA) in the recipient's plasma and/or urine may be a better indicator of acute rejection. We evaluated digital PCR (dPCR) as a system for monitoring graft status using single nucleotide polymorphism (SNP)-based detection of donor DNA in plasma or urine...
March 2017: Genomics & Informatics
https://www.readbyqxmd.com/read/28404592/accounting-for-oxygen-in-the-renal-cortex-a-computational-study-of-factors-that-predispose-the-cortex-to-hypoxia
#17
Chang-Joon Lee, Bruce S Gardiner, Jennifer P Ngo, Saptarshi Kar, Roger G Evans, David W Smith
We develop a pseudo-3D model of oxygen transport for the renal cortex of the rat, incorporating both the axial and radial geometry of the pre-glomerular circulation and quantitative information regarding the surface areas and transport from the vasculature and renal corpuscles. The computational model was validated by simulating four sets of published experimental studies of renal oxygenation in rats. Under the control conditions, the predicted cortical tissue oxygen tension (PtO2) or microvascular oxygen tension (µPO2) were within ± 1 standard error of the mean value (SEM) observed experimentally...
April 12, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28398210/out-of-field-doses-from-pediatric-craniospinal-irradiations-using-3d-crt-imrt-helical-tomotherapy-and-electron-based-therapy
#18
Marijke De Saint-Hubert, Dirk Verellen, Kenneth Poels, Wouter Crijns, Federica Magliona, Tom Depuydt, Filip Vanhavere, Lara Struelens
Medulloblastoma treatment involves irradiation of the entire central nervous system, i.e craniospinal irradiation (CSI). This is associated with significant exposure of large volumes of healthy tissue with a growing concern regarding treatment associated side effects. The current study compares out-of-field organ doses in children receiving CSI with three-dimensional-conformal radiotherapy (3D-CRT), intensity modulated radiotherapy (IMRT), helical tomotherapy (HT) and an electron-based technique, including as well radiation doses resulting from imaging performed during treatment...
April 11, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28395417/-dosimetry-verification-of-radioactive-seed-implantation-with-3d-printing-template-and-ct-guidance-for-paravertebral-retroperitoneal-malignant-tumor
#19
Z Ji, Y L Jiang, F X Guo, R Peng, H T Sun, J H Fan, J J Wang
Objective: To compare the dose distributions of postoperative plans with preoperative plans for seeds implantations of paravertebral/retroperitoneal tumors assisted by 3D printing guide template and CT guidance, explore the effects of the technology for seeds implantations in dosimetry level and provide data support for the optimization and standardization in seeds implantation. Methods: Between December 2015 and July 2016, a total of 10 patients with paravertebral/retroperitoneal tumors (12 lesions) received 3D printing template assist radioactive seeds implantations in department of radiation oncology of Peking University Third Hospital, and included in the study...
April 4, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28394950/generation-and-characterization-of-an-antagonistic-monoclonal-antibody-against-an-extracellular-domain-of-mouse-dp2-crth2-gpr44-receptors-for-prostaglandin-d2
#20
Nanae Nagata, Hiroko Iwanari, Hidetoshi Kumagai, Osamu Kusano-Arai, Yuichi Ikeda, Kosuke Aritake, Takao Hamakubo, Yoshihiro Urade
Prostaglandin D2 (PGD2) is a lipid mediator involved in sleep regulation and inflammation. PGD2 interacts with 2 types of G protein-coupled receptors, DP1 and DP2/CRTH2 (chemoattractant receptor homologous molecule expressed on T helper type 2 cells)/GPR44 to show a variety of biological effects. DP1 activation leads to Gs-mediated elevation of the intracellular cAMP level, whereas activation of DP2 decreases this level via the Gi pathway; and it also induces G protein-independent, arrestin-mediated cellular responses...
2017: PloS One
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