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https://www.readbyqxmd.com/read/28524367/transplantation-of-human-villous-trophoblasts-preserves-cardiac-function-in-mice-with-acute-myocardial-infarction
#1
Zegen Wang, Ningzheng Dong, Yayan Niu, Zhiwei Zhang, Ce Zhang, Meng Liu, Tiantian Zhou, Qingyu Wu, Ke Cheng
Over the past decade, cell therapies have provided promising strategies for the treatment of ischaemic cardiomyopathy. Particularly, the beneficial effects of stem cells, including bone marrow stem cells (BMSCs), endothelial progenitor cells (EPCs), mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs), have been demonstrated by substantial preclinical and clinical studies. Nevertheless stem cell therapy is not always safe and effective. Hence, there is an urgent need for alternative sources of cells to promote cardiac regeneration...
May 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28520507/gold-nanoparticles-enhance-the-differentiation-of-embryonic-stem-cells-into-dopaminergic-neurons-via-mtor-p70s6k-pathway
#2
Min Wei, Song Li, Zhaofei Yang, Wei Zheng, Weidong Le
AIM: This study aimed to investigate the effect of gold nanoparticles (AuNPs) on differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DA) neurons and explore the possible underlying molecular mechanisms. MATERIALS & METHODS: The efficiency of AuNPs on DA neuron differentiation was evaluated by observing fluorescence in TH promoter-engineered GFP-reporter ESCs, western blot and real-time PCR. The possible signal pathway was determined by western blot...
May 18, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28518115/analysis-of-retinoic-acid-induced-neural-differentiation-of-mouse-embryonic-stem-cells-in-two-and-three-dimensional-embryoid-bodies
#3
Junning Yang, Chuanshen Wu, Ioana Stefanescu, Arie Horowitz
Mouse embryonic stem cells (ESCs) isolated from the inner mass of the blastocyst (typically at day E3.5), can be used as in vitro model system for studying early embryonic development. In the absence of leukemia inhibitory factor (LIF), ESCs differentiate by default into neural precursor cells. They can be amassed into a three dimensional (3D) spherical aggregate termed embryoid body (EB) due to its similarity to the early stage embryo. EBs can be seeded on fibronectin-coated coverslips, where they expand by growing two dimensional (2D) extensions, or implanted in 3D collagen matrices where they continue growing as spheroids, and differentiate into the three germ layers: endodermal, mesodermal, and ectodermal...
April 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28515460/chlamydia-trachomatis-infection-of-human-endometrial-stromal-cells-induces-defective-decidualisation-and-chemokine-release
#4
Sevi Giakoumelou, Nick Wheelhouse, Jeremy Brown, Jean Wade, Ioannis Simitsidellis, Douglas Gibson, Philippa T K Saunders, Patrick Horner, Gary Entrican, Sarah E M Howie, Andrew W Horne
Miscarriage affects ~20% of pregnancies and maternal infections account for ~15% of early miscarriages. Chlamydia trachomatis (Ct) has been associated with miscarriage but the underlying mechanisms are unknown. Successful implantation requires endometrial stromal cell (ESC) decidualisation. Maintenance of pregnancy requires angiogenesis, establishment of the correct cellular milieu and trophoblast invasion, all of which involve the action of chemokines. Our objective was to determine whether Ct infection impacts upon ESC decidualisation and chemokine secretion...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28511663/basic-fibroblast-growth-factor-reduces-scar-by-inhibiting-the-differentiation-of-epidermal-stem-cells-to-myofibroblasts-via-the-notch1-jagged1-pathway
#5
Peng Wang, Bin Shu, Yingbin Xu, Jiayuan Zhu, Jian Liu, Ziheng Zhou, Lei Chen, Jingling Zhao, Xusheng Liu, Shaohai Qi, Kun Xiong, Julin Xie
BACKGROUND: Basic fibroblast growth factor (bFGF) plays an important role in promoting wound healing and reducing scar, but the possible molecular mechanisms are still unclear. Our previous studies have found that activating the Notch1/Jagged1 pathway can inhibit the differentiation of epidermal stem cells (ESCs) to myofibroblasts (MFB). Herein, we document that bFGF reduces scar by inhibiting the differentiation of ESCs to MFB via activating the Notch1/Jagged1 pathway. METHODS: In in-vitro study, ESCs were isolated from 10 neonatal SD rats (1-3 days old), cultured in keratinocyte serum-free medium, and divided into six groups: bFGF group, bFGF + SU5402 group, bFGF + DAPT group, siJagged1 group, bFGF + siJagged1 group, and control group...
May 16, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28511325/-feasibility-of-the-2014-european-guidelines-risk-prediction-model-for-sudden-cardiac-death-in-hypertrophic-cardiomyopathy-in-chinese-patients
#6
S H Zhu, Y Li, W Huang, H Jiang, S N Li, L Chen, S Huang, H S Yu, B Xu
Objective: Exploring the feasibility of the 2014 European Society of Cardiology(ESC)guideline's risk prediction model for sudden cardiac death in hypertrophic cardiomyopathy (HCM Risk-SCD) in Chinese patients. Methods: The study population consisted of a consecutive cohort of 172 Chinese patients with HCM without prior sudden cardiac death (SCD) event who were in patients in Nanjing Drum Tower Hospital from December 2010 to October 2015.The endpoint event was a composite of SCD and appropriate implantable cardioverter-defibrillator (ICD) therapy...
May 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28509913/optimization-of-a-polydopamine-pd-based-coating-method-and-polydimethylsiloxane-pdms-substrates-for-improved-mouse-embryonic-stem-cell-esc-pluripotency-maintenance-and-cardiac-differentiation
#7
Jiayin Fu, Yon Jin Chuah, Wee Tong Ang, Nan Zheng, Dong-An Wang
Myocardiocyte derived from pluripotent stem cells, such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), is a promising cell source for cardiac tissue engineering. Combined with microfluidic technologies, a heart-on-a-chip is very likely to be developed and function as a platform for high throughput drug screening. Polydimethylsiloxane (PDMS) silicone elastomer is a widely-used biomaterial for the investigation of cell-substrate interactions and biochip fabrication. However, the intrinsic PDMS surface hydrophobicity inhibits cell adhesion on the PDMS surface, and PDMS surface modification is required for effective cell adhesion...
May 16, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28508269/erratum-to-guidelines-in-review-comparison-between-aha-acc-and-esc-guidelines-for-the-management-of-patients-with-ventricular-arrhythmias-and-the-prevention-of-sudden-cardiac-death
#8
Alejandro Velasco, Jim Stirrup, Eliana Reyes, Fadi G Hage
No abstract text is available yet for this article.
May 15, 2017: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/28500370/long-term-prognostic-performance-of-low-dose-coronary-computed-tomography-angiography-with-prospective-electrocardiogram-triggering
#9
Olivier F Clerc, Basil P Kaufmann, Mathias Possner, Riccardo Liga, Jan Vontobel, Fran Mikulicic, Christoph Gräni, Dominik C Benz, Tobias A Fuchs, Julia Stehli, Aju P Pazhenkottil, Oliver Gaemperli, Philipp A Kaufmann, Ronny R Buechel
OBJECTIVES: To assess long-term prognosis after low-dose 64-slice coronary computed tomography angiography (CCTA) using prospective electrocardiogram-triggering. METHODS: We included 434 consecutive patients with suspected or known coronary artery disease referred for low-dose CCTA. Patients were classified as normal, with non-obstructive or obstructive lesions, or previously revascularized. Coronary artery calcium score (CACS) was assessed in 223 patients. Follow-up was obtained regarding major adverse cardiac events (MACE): cardiac death, myocardial infarction and elective revascularization...
May 12, 2017: European Radiology
https://www.readbyqxmd.com/read/28498092/barriers-to-esc-guideline-implementation-results-of-a-survey-from-the-european-council-on-cardiovascular-nursing-and-allied-professions-ccnap
#10
Gabrielle McKee, Mary Kerins, Glenys Hamilton, Tina Hansen, Jeroen Hendriks, Eleni Kletsiou, Ekaterini Lambrinou, Catriona Jennings, Donna Fitzsimons
BACKGROUND: The European Society of Cardiology (ESC) has a comprehensive clinical guideline development programme, relevant for all clinicians. However, implementation of guidelines is not always optimal. AIM: The aim of this study was to determine nurses' and allied professionals' awareness and barriers regarding clinical guideline implementation. METHODS: A cross-sectional survey was administrated online and in print at EuroHeartCare 2015...
May 1, 2017: European Journal of Cardiovascular Nursing
https://www.readbyqxmd.com/read/28496549/phosphodiesterase-5-inhibitors-improve-clinical-outcomes-exercise-capacity-and-pulmonary-hemodynamics-in-patients-with-heart-failure-with-reduced-left-ventricular-ejection-fraction-a-meta-analysis
#11
Renato De Vecchis, Arturo Cesaro, Carmelina Ariano, Anna Giasi, Carmela Cioppa
BACKGROUND: Several studies have compared the use of phosphodiesterase-5 (PDE5) inhibitors sildenafil or udenafil with the placebo in patients suffering from pulmonary hypertension (PH) due to left chronic heart failure (CHF), corresponding to group 2 (PH due to left heart disease) of the PH classification (according to 2015 ESC/ERS guidelines for the diagnosis and treatment of PH). The results of the use of PDE5 inhibitors in the PH due to left heart disease were inconsistent and heterogeneous...
June 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/28495881/dynamic-expression-and-regulatory-mechanism-of-tgf-%C3%AE-signaling-in-chicken-embryonic-stem-cells-differentiating-into-spermatogonial-stem-cells
#12
Qisheng Zuo, Kai Jin, Yani Zhang, Jiuzhou Song, Bichun Li
This study investigated the dynamic expression and regulatory mechanism of TGF-β signaling involved in embryonic stem cells (ESCs) differentiation into male germ cells. Candidate genes involved in TGF-β signaling pathway were screened from RNA-seq, which were further validated by quantitative real time PCR(qRT-PCR). Bone morphogenetic protein 4 (BMP4) was used to induce differentiation of ESCs in vitro Inhibition of TGF-β signaling pathway was reflected by western blot of SMAD 2 and SMAD 5 expression. Differentiating efficiency of germ cells was evaluated by immunofluorescence and fluorescence activated cell sorting(FACS)...
May 11, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28495691/screening-for-pulmonary-arterial-hypertension-in-an-unselected-prospective-systemic-sclerosis-cohort
#13
Els Vandecasteele, Benny Drieghe, Karin Melsens, Kristof Thevissen, Michel De Pauw, Ellen Deschepper, Saskia Decuman, Carolien Bonroy, Yves Piette, Filip De Keyser, Guy Brusselle, Vanessa Smith
Screening for pulmonary arterial hypertension (PAH) in systemic sclerosis (SSc) improves outcomes. The DETECT screening algorithm is recommended in a high-risk SSc subgroup. This study aims to compare prospectively the positive predictive value of screening using the DETECT algorithm and the 2009 European Society of Cardiology/European Respiratory Society (ESC/ERS) guidelines, and to compare their cost-effectiveness in an unselected, day-to-day SSc population. Post hoc, screening according to the 2015 ESC/ERS guidelines using echocardiographic parameters alone ("2015 echo screening") or combined with the DETECT algorithm ("2015 combined screening") in high-risk subjects was analysed...
May 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28494860/overexpression-of-trophoblast-stem-cell-enriched-micrornas-promotes-trophoblast-fate-in-embryonic-stem-cells
#14
Ursula Nosi, Fredrik Lanner, Tsu Huang, Brian Cox
The first cell fate choice of the preimplantation embryo generates the extraembryonic trophoblast and embryonic epiblast lineages. Embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) can be utilized to investigate molecular mechanisms of this first cell fate decision. It has been established that ESCs can be induced to acquire trophoblast lineage characteristics upon manipulation of lineage-determining transcription factors. Here, we have interrogated the potential of microRNAs (miRNAs) to drive trans-differentiation of ESCs into the trophoblast lineage...
May 9, 2017: Cell Reports
https://www.readbyqxmd.com/read/28494789/micrornas-important-regulators-of-stem-cells
#15
REVIEW
Na Li, Bo Long, Wei Han, Shumin Yuan, Kun Wang
Stem cells are undifferentiated cells and have multi-lineage differentiation potential. Generally, stem cells are classified into adult stem cells, embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Stem cells have great potential in clinical therapy due to their pluripotency and self-renewal ability. microRNAs (miRNAs) are small non-coding RNAs which are evolutionarily conserved and participate in the pathogenesis of many diseases, cell cycle regulation, apoptosis, aging, cell fate decisions, and different signaling pathways...
May 11, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28494174/human-endometrial-stromal-cell-plasticity-reversible-sflt1-expression-negatively-coincides-with-decidualization
#16
Hanh N Cottrell, JuanJuan Wu, Bassam H Rimawi, Juan Manuel Duran, Jessica B Spencer, Neil Sidell, Augustine Rajakumar
Preeclampsia (PE) is a major complication of pregnancy in which the placenta is known to have shallow implantation into the uterine decidua. Studies have implicated soluble fms-like tyrosine kinase-1 (sFlt1), a soluble vascular endothelial growth factor (VEGF) receptor protein, in the pathogenesis of PE. sFlt1 has the ability to bind to and neutralize the angiogenic functions of VEGF and placental growth factor (PlGF). The presence of sFlt1 and its action in the endometrium is yet to be determined. We hypothesize that endometrial stromal cells (ESC) at the maternal-fetal interface may play a role in sFlt-1 regulation during pregnancy...
May 11, 2017: Hypertension in Pregnancy
https://www.readbyqxmd.com/read/28487853/promising-approaches-to-optimize-the-biological-properties-of-the-antimicrobial-peptide-esculentin-1a-1-21-nh2-amino-acids-substitution-and-conjugation-to-nanoparticles
#17
REVIEW
Bruno Casciaro, Floriana Cappiello, Mauro Cacciafesta, Maria Luisa Mangoni
Antimicrobial peptides (AMPs) represent an interesting class of molecules with expanding biological properties which make them a viable alternative for the development of future antibiotic drugs. However, for this purpose, some limitations must be overcome: (i) the poor biostability due to enzymatic degradation; (ii) the cytotoxicity at concentrations slightly higher than the therapeutic dosages; and (iii) the inefficient delivery to the target site at effective concentrations. Recently, a derivative of the frog skin AMP esculentin-1a, named esculentin-1a(1-21)NH2, [Esc(1-21): GIFSKLAGKKIKNLLISGLKG-NH2] has been found to have a potent activity against the Gram-negative bacterium Pseudomonas aeruginosa; a slightly weaker activity against Gram-positive bacteria and interesting immunomodulatory properties...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28487382/nf45-and-nf90-nf110-coordinately-regulate-esc-pluripotency-and-differentiation
#18
Julia Ye, Hu Jin, Aleksandr Pankov, Jun Song, Robert Blelloch
While years of investigation have elucidated many aspects of embryonic stem cell (ESC) regulation, the contributions of post-transcriptional and translational mechanisms to the pluripotency network remain largely unexplored. In particular, little is known in ESCs about the function of RNA binding proteins (RBPs), the protein agents of post-transcriptional regulation. We performed an unbiased RNAi screen of RBPs in an ESC differentiation assay and identified two related genes, NF45 (Ilf2) and NF90/NF110 (Ilf3), whose knockdown promoted differentiation to an epiblast-like state...
May 9, 2017: RNA
https://www.readbyqxmd.com/read/28487017/yap-mediates-human-decidualization-of-the-uterine-endometrial-stromal-cells
#19
Hengxi Chen, Yong Song, Shiyuan Yang, Jing Fu, Xue Feng, Wei Huang
INTRODUCTION: The decidualization of uterine endometrial stromal cells (ESCs) is critical for the successful establishment and maintenance of pregnancy and involves extensive cell proliferation and differentiation. A newly established signaling pathway, the Hippo/Yes-associated protein (YAP) pathway, plays a critical role in these proliferation processes. Our previous study demonstrated that YAP is expressed in human ESCs. However, its role in decidualization remains unclear. The objective of the present study was to explore the role of YAP in the decidualization of human ESCs...
May 2017: Placenta
https://www.readbyqxmd.com/read/28483982/akt3-is-responsible-for-the-survival-and-proliferation-of-embryonic-stem-cells
#20
Ling Wang, Delun Huang, Zongliang Jiang, Yan Luo, Carol Norris, Ming Zhang, Xiuchun Tian, Young Tang
The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB/Akt) pathway plays an important role in regulating cell proliferation, metabolism, and survival. However, the distinct roles of Akt isoforms - Akt1, Akt2, and Akt3 in pluripotent stem cell maintenance are not fully defined. Using mouse embryonic stem cells (ESCs), we show that direct inhibition of Akt activity leads to ESC apoptosis. The Akt3, but not Akt1 or Akt2 activity specifically regulates this effect. Inhibiting Akt3 also leads to a cell cycle arrest at G1 phase...
May 8, 2017: Biology Open
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