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Carbohydrate metabolism

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https://www.readbyqxmd.com/read/28738195/proteomic-and-genetics-insights-on-the-response-of-the-bacteriocinogenic-lactobacillus-sakei-crl1862-during-biofilm-formation-on-stainless-steel-surface-at-10%C3%A2-c
#1
Mariana Pérez-Ibarreche, Lucía M Mendoza, Graciela Vignolo, Silvina Fadda
Some lactic acid bacteria have the ability to form biofilms on food-industry surfaces and this property could be used to control food pathogens colonization. Lactobacillus sakei CR1862 was selected considering its bacteriocinogenic nature and ability to adhere to abiotic surfaces at low temperatures. In this study, the proteome of L. sakei CRL1862 grown either under biofilm on stainless steel surface and planktonic modes of growth at 10°C, was investigated. Using two-dimensional gel electrophoresis, 29 out of 43 statistically significant spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
July 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28737859/association-between-dietary-glycemic-load-and-metabolic-syndrome-in-obese-children-and-adolescents
#2
Angela Cornejo-Monthedoro, Isel Negreiros-Sánchez, Carlos Del Águila, Marlit Ysla-Marquillo, Percy Mayta-Tristán
OBJETIVE: To study the association between the metabolic syndrome (MS) and the glycemic load (GL) of food intake among obese children and adolescents seen in consultation by the endocrinology team in a pediatric referral hospital in Lima, Peru. POPULATION AND METHOD: Cross-sectional study among obese children and adolescents (body mass index ≥ 95 percentile), 10-15 years old. The MS was classified according to criteria of the International Diabetes Federation...
August 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28737202/-euglycemic-ketosis-in-an-adolescent-with-type-1-diabetes-on-insulin-and-dapaglifozin-case-report
#3
Marcia Pereyra A, Cristhian Ramírez, Rossana Román
Dapagliflozin, an insulin-independent sodium-glucose cotransporter 2 inhibitor (SGLT2-I) induces glycosuria and reduces hyperglycemia in adults with type 2 diabetes. OBJECTIVE: To present an “euglycemic” diabetic ketosis in an adolescent with type 1 diabetes (T1D) receiving dapagliflozin, to alert about the risk of a drug not approved in children nor in T1D. CASE REPORT: A 17 years old adolescent with T1D during 9 years, was started on dapagliflozin 10 mg / day to reduce insulin dose and weight...
June 2017: Revista Chilena de Pediatría
https://www.readbyqxmd.com/read/28735404/porous-graphitic-carbon-liquid-chromatography-mass-spectrometry-analysis-of-drought-stress-responsive-raffinose-family-oligosaccharides-in-plant-tissues
#4
Tiago F Jorge, Maria H Florêncio, Carla António
Drought is a major limiting factor in agriculture and responsible for dramatic crop yield losses worldwide. The adjustment of the metabolic status via accumulation of drought stress-responsive osmolytes is one of the many strategies that some plants have developed to cope with water deficit conditions. Osmolytes are highly polar compounds, analysis of whcih is difficult with typical reversed-phase chromatography. Porous graphitic carbon (PGC) has shown to be a suitable alternative to reversed-phase stationary phases for the analysis of highly polar compounds typically found in the plant metabolome...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28733872/metabolic-profiles-of-flooding-tolerant-mechanism-in-early-stage-soybean-responding-to-initial-stress
#5
Xin Wang, Wei Zhu, Akiko Hashiguchi, Minoru Nishimura, Jingkui Tian, Setsuko Komatsu
Metabolomic analysis of flooding-tolerant mutant and abscisic acid-treated soybeans suggests that accumulated fructose might play a role in initial flooding tolerance through regulation of hexokinase and phosphofructokinase. Soybean is sensitive to flooding stress, which markedly reduces plant growth. To explore the mechanism underlying initial-flooding tolerance in soybean, mass spectrometry-based metabolomic analysis was performed using flooding-tolerant mutant and abscisic-acid treated soybeans. Among the commonly-identified metabolites in both flooding-tolerant materials, metabolites involved in carbohydrate and organic acid displayed same profile at initial-flooding stress...
July 21, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28733241/live-imaging-using-a-fret-glucose-sensor-reveals-glucose-delivery-to-all-cell-types-in-the-drosophila-brain
#6
Anne Volkenhoff, Johannes Hirrlinger, Johannes M Kappel, Christian Klämbt, Stefanie Schirmeier
All complex nervous systems are metabolically separated from circulation by a blood-brain barrier (BBB) that prevents uncontrolled leakage of solutes into the brain. Thus, all metabolites needed to sustain energy homeostasis must be transported across this BBB. In invertebrates, such as Drosophila, the major carbohydrate in circulation is the disaccharide trehalose and specific trehalose transporters are expressed by the glial BBB. Here we analyzed whether glucose is able to contribute to energy homeostasis in Drosophila...
July 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28733104/comparative-shoot-proteome-analysis-of-two-potato-solanum-tuberosum-l-genotypes-contrasting-in-nitrogen-deficiency-responses-in-vitro
#7
Philipp Meise, Anna Maria Jozefowicz, Ralf Uptmoor, Hans-Peter Mock, Frank Ordon, Annegret Schum
Aiming at a better understanding of the physiological and biochemical background of nitrogen use efficiency, alterations in the shoot proteome under N-deficiency were investigated in two contrasting potato genotypes grown in vitro with 60 and 7.5mM N, respectively. A gel based proteomic approach was applied to identify candidate proteins associated with genotype specific responses to N-deficiency. 21% of the detected proteins differed in abundance between the two genotypes. Between control and N-deficiency conditions 19...
July 18, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28731470/a-genomic-perspective-on-stoichiometric-regulation-of-soil-carbon-cycling
#8
Wyatt H Hartman, Rongzhong Ye, William R Horwath, Susannah G Tringe
Similar to plant growth, soil carbon (C) cycling is constrained by the availability of nitrogen (N) and phosphorus (P). We hypothesized that stoichiometric control over soil microbial C cycling may be shaped by functional guilds with distinct nutrient substrate preferences. Across a series of rice fields spanning 5-25% soil C (N:P from 1:12 to 1:70), C turnover was best correlated with P availability and increased with experimental N addition only in lower C (mineral) soils with N:P⩽16. Microbial community membership also varied with soil stoichiometry but not with N addition...
July 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28729282/draft-genome-sequence-of-actinomycessucciniciruminis-strain-am4-t-isolated-from-cow-rumen-fluid
#9
Susakul Palakawong Na Ayudthaya, Bastian Hornung, Adithi Ravikumar Varadarajan, Wendy Plugge, Caroline M Plugge
Actinomyces succiniciruminis strain Am4(T), isolated from cow rumen fluid, can metabolize a range of substrates including complex carbohydrates to organic acids. Here, we report a 3.33-Mbp draft genome of Actinomyces succiniciruminis.
July 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28728708/effect-of-partitioning-the-nonfiber-carbohydrate-fraction-and-neutral-detergent-fiber-method-on-digestibility-of-carbohydrates-by-dairy-cows
#10
A W Tebbe, M J Faulkner, W P Weiss
Many nutrition models rely on summative equations to estimate feed and diet energy concentrations. These models partition feed into nutrient fractions and multiply the fractions by their estimated true digestibility, and the digestible mass provided by each fraction is then summed and converted to an energy value. Nonfiber carbohydrate (NFC) is used in many models. Although it behaves as a nutritionally uniform fraction, it is a heterogeneous mixture of components. To reduce the heterogeneity, we partitioned NFC into starch and residual organic matter (ROM), which is calculated as 100 - CP - LCFA - ash - starch - NDF, where crude protein (CP), long-chain fatty acids (LCFA), ash, starch, and neutral detergent fiber (NDF) are a percentage of DM...
August 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28727805/turgor-responsive-starch-phosphorylation-in-oryza-sativa-stems-a-primary-event-of-starch-degradation-associated-with-grain-filling-ability
#11
Hiroshi Wada, Chisato Masumoto-Kubo, Koichi Tsutsumi, Hiroshi Nonami, Fukuyo Tanaka, Haruka Okada, Rosa Erra-Balsells, Kenzo Hiraoka, Taiken Nakashima, Makoto Hakata, Satoshi Morita
Grain filling ability is mainly affected by the translocation of carbohydrates generated from temporarily stored stem starch in most field crops including rice (Oryza sativa L.). The partitioning of non-structural stem carbohydrates has been recognized as an important trait for raising the yield ceiling, yet we still do not fully understand how carbohydrate partitioning occurs in the stems. In this study, two rice subspecies that exhibit different patterns of non-structural stem carbohydrates partitioning, a japonica-dominant cultivar, Momiroman, and an indica-dominant cultivar, Hokuriku 193, were used as the model system to study the relationship between turgor pressure and metabolic regulation of non-structural stem carbohydrates, by combining the water status measurement with gene expression analysis and a dynamic prefixed 13C tracer analysis using a mass spectrometer...
2017: PloS One
https://www.readbyqxmd.com/read/28727777/8-oxoguanine-dna-glycosylase-ogg1-deficiency-elicits-coordinated-changes-in-lipid-and-mitochondrial-metabolism-in-muscle
#12
Vladimir Vartanian, Jana Tumova, Pawel Dobrzyn, Agnieszka Dobrzyn, Yusaku Nakabeppu, R Stephen Lloyd, Harini Sampath
Oxidative stress resulting from endogenous and exogenous sources causes damage to cellular components, including genomic and mitochondrial DNA. Oxidative DNA damage is primarily repaired via the base excision repair pathway that is initiated by DNA glycosylases. 8-oxoguanine DNA glycosylase (OGG1) recognizes and cleaves oxidized and ring-fragmented purines, including 8-oxoguanine, the most commonly formed oxidative DNA lesion. Mice lacking the OGG1 gene product are prone to multiple features of the metabolic syndrome, including high-fat diet-induced obesity, hepatic steatosis, and insulin resistance...
2017: PloS One
https://www.readbyqxmd.com/read/28727091/endogenous-fat-loss-and-true-total-tract-digestibility-of-poultry-fat-in-adult-dogs
#13
F R Marx, L Trevizan, F M O B Saad, K G Lisenko, J S Reis, A M Kessler
Fat plays an important role in the carnivore diet. It has high digestibility and high energy content and likely improves digestibility of the diet when present in greater amounts, suggesting that endogenous fat adversely affects the apparent digestibility of fat in low-fat diets. This study aimed to determine the endogenous fat loss (EFL) and true total tract digestibility (TTTD) of fat in dogs by using diets having different fat:carbohydrate ratios. Furthermore, the apparent total tract digestibility (ATTD) of macronutrients and energy, food intake, fecal output, and stool quality were evaluated...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726105/changes-in-levels-of-metabolic-pathway-gene-expression-under-conditions-of-clear-cell-renal-carcinoma
#14
V Yu Bashmakov, T M Gorbacheva, A V Panevina, S A Solodskikh, I P Moshurov, A A Mikhaylov, A Yu Maslov, V N Popov
A whole-transcriptome analysis of gene expression in six samples of clear cell renal cancer was performed. Using bioinformatics methods, we established a relationship between gene expression data and changes in activity of metabolic pathways: in this cancer type, the expression of genes involved in the metabolism of carbohydrates, lipids, and amino acids as well as in energy metabolism changed most significantly.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28724970/classification-of-skin-phenotypes-caused-by-diabetes-mellitus-using-complex-scattering-parameters-in-the-millimeter-wave-frequency-range
#15
Fabian Dornuf, Pedro Martín-Mateos, Blanca Duarte, Bernhard Hils, Oscar Elias Bonilla-Manrique, Fernando Larcher, Pablo Acedo, Viktor Krozer
The pathological skin phenotype caused by hyperglycemia is an important indicator for the progress of diabetes mellitus. An early detection of diabetes assures an early intervention to regulate the carbohydrate metabolism. In this publication a non-invasive detection principle based on the measurement of complex scattering parameters in the millimeter-wave frequency range is presented. The measurement principle provides evidence of the applicability for the identification of different glycemic states in animal models...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724349/16s-rrna-gene-based-association-study-identified-microbial-taxa-associated-with-pork-intramuscular-fat-content-in-feces-and-cecum-lumen
#16
Shaoming Fang, Xingwei Xiong, Ying Su, Lusheng Huang, Congying Chen
BACKGROUND: Intramuscular fat (IMF) that deposits among muscle fibers or within muscle cells is an important meat quality trait in pigs. Previous studies observed the effects of dietary nutrients and additives on improving the pork IMF. Gut microbiome plays an important role in host metabolism and energy harvest. Whether gut microbiota exerts effect on IMF remains unknown. RESULTS: In this study, we investigated the microbial community structure of 500 samples from porcine cecum and feces using high-throughput 16S rRNA gene sequencing...
July 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28724173/a-comparative-study-between-myo-inositol-and-metformin-in-the-treatment-of-insulin-resistant-women
#17
K Nas, L Tűű
OBJECTIVE: Insulin resistance (IR) is a common disorder, which can impair female fertility and is characterized by phenotypic heterogeneity. Life-style treatment and insulin sensitizers are commonly used in the management of women with IR and consequent hyperinsulinemia, in order to restore the normal endocrinological and clinical parameters. Metformin (MET) is considered one of the first approaches to this pathology but new evidences are showing promising results using myo-inositol (MYO) in the management of this pathology...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28724055/bone-turnover-after-bariatric-surgery
#18
Thalita Lima Melo, Leila Froeder, Leandro da Cunha Baia, Ita Pfeferman Heilberg
Objective: The aim of the present study was to evaluate parameters of bone and mineral metabolism after bariatric surgery. Subjects and methods: This sectional study included data from medical records from 61 bariatric surgery (BS) patients (minimum period of 6 months after the procedure) and from 30 class II and III obese patients as a control group (Cont), consisting of daily dietary intake of macronutrients, calcium and sodium, serum 25(OH)D and parathyroid hormone (PTH) and other biochemical serum and urinary parameters...
July 13, 2017: Archives of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28723843/no-superior-adaptations-to-carbohydrate-periodization-in-elite-endurance-athletes
#19
Kasper Degn Gejl, Line Thams, Mette Hansen, Torben Rokkedal-Lausch, Peter Plomgaard, Lars Nybo, Filip J Larsen, Daniele A Cardinale, Kurt Jensen, Hans-Christer Holmberg, Kristian Vissing, Niels Ørtenblad
PURPOSE: The present study investigated the effects of periodic CHO restriction on endurance performance and metabolic markers in elite endurance athletes. METHODS: Twenty-six male elite endurance athletes (VO2max: 65.0 ml O2[BULLET OPERATOR]kg[BULLET OPERATOR]min) completed 4 weeks of regular endurance training, while matched and randomized into two groups training with (Low) or without (High) carbohydrate (CHO) manipulation three days a week. The CHO manipulation days consisted of a 1-hr high intensity bike session in the morning, recovery for 7 hrs while consuming isocaloric diets containing either high CHO (414±2...
July 19, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28722406/possible-molecular-mechanisms-by-which-an-essential-oil-blend-from-star-anise-rosemary-thyme-and-oregano-and-saponins-increase-the-performance-and-ileal-protein-digestibility-of-growing-broilers
#20
Henry Reyer, Jürgen Zentek, Klaus Männer, Ibrahim M I Youssef, Tobias Aumüller, Julian Weghuber, Klaus Wimmers, Andreas Müller
Phytogenic feed additives represent a potential alternative to antibiotics with attributed health and growth promoting effects. Chickens supplemented with an essential oil blend, a Quillaja saponin blend or a combination of both phytogenic preparations showed a comprehensively and significantly improved apparent ileal digestibility of crude protein and amino acids compared to control birds. Accordingly, holistic transcriptomic analyses of jejunum and liver samples indicated alterations of macromolecule transporters and processing pathways likely culminating in an increased uptake and metabolizing of carbohydrates and fatty acids...
July 19, 2017: Journal of Agricultural and Food Chemistry
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