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https://www.readbyqxmd.com/read/28421415/a-tyrosine-residue-along-with-a-glutamic-acid-of-the-omega-like-loop-governs-the-beta-lactamase-activity-of-msmeg_4455-in-mycobacterium-smegmatis
#1
Ankita Bansal, Debasish Kar, Satya Deo Pandey, Ashok Matcha, N Ganesh Kumar, Soshina Nathan, Anindya S Ghosh
Mycobacterial beta-lactamases are involved in exerting beta-lactam resistance, though many of these proteins remain uncharacterized. Here, we have characterized MSMEG_4455 of Mycobacterium smegmatis as a beta-lactamase using molecular, biochemical and mutational techniques. To elucidate its nature in vivo and in vitro, and to predict its structure-function relationship in silico analysis is done. The MSMEG_4455 is cloned and expressed ectopically in a beta-lactamase deficient Escherichia coli mutant to establish the in vivo beta-lactamase like nature via minimum inhibitory concentration (MIC) determination...
April 19, 2017: Protein Journal
https://www.readbyqxmd.com/read/28343386/characterization-of-the-potent-odorants-contributing-to-the-characteristic-aroma-of-matcha-by-gas-chromatography-olfactometry-techniques
#2
Ryoko Baba, Yohei Amano, Yoshiyuki Wada, Kenji Kumazawa
The odorants contributing to the characteristic aroma of Matcha were investigated by analysis of the headspace samples and the volatile fractions prepared by a combination of solvent extraction and the SAFE techniques using three Matcha powders of different grades (high, medium, and low). Gas chromatography-olfactometry of the headspace samples (GCO-H) and the aroma extract dilution analysis (AEDA) applied to the volatile fractions revealed 16 (FD factor ≥1) and 39 (FD factor ≥4(3)) odor-active peaks, respectively...
March 25, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28327441/structure-based-design-synthesis-and-biological-evaluation-of-amino-phosphonate-derivatives-as-human-glucokinase-activators
#3
Nanda Kumar Yellapu, Ravendra Babu Kilaru, Nagaraju Chamarthi, Sarma Pvgk, Bhaskar Matcha
Glucokinase (GK) is a potential therapeutic target of type 2 diabetes and GK activators (GKAs) represent a promising class of small organic molecules which enhance GK activity. Based on the configuration and conformation of the allosteric site of GK, we have designed a novel class of amino phosphonate derivatives in order to develop potent GKAs. The QSAR model developed using numerous descriptors revealed its potential with the best effective statistical values of RMSE=1.52 and r(2)=0.30. Moreover, application of this model on the present test set GKAs proved to be worthy to predict their activities as a better linear relationship was observed with RMSE=0...
March 2, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28249298/the-modern-japanese-color-lexicon
#4
Ichiro Kuriki, Ryan Lange, Yumiko Muto, Angela M Brown, Kazuho Fukuda, Rumi Tokunaga, Delwin T Lindsey, Keiji Uchikawa, Satoshi Shioiri
Despite numerous prior studies, important questions about the Japanese color lexicon persist, particularly about the number of Japanese basic color terms and their deployment across color space. Here, 57 native Japanese speakers provided monolexemic terms for 320 chromatic and 10 achromatic Munsell color samples. Through k-means cluster analysis we revealed 16 statistically distinct Japanese chromatic categories. These included eight chromatic basic color terms (aka/red, ki/yellow, midori/green, ao/blue, pink, orange, cha/brown, and murasaki/purple) plus eight additional terms: mizu ("water")/light blue, hada ("skin tone")/peach, kon ("indigo")/dark blue, matcha ("green tea")/yellow-green, enji/maroon, oudo ("sand or mud")/mustard, yamabuki ("globeflower")/gold, and cream...
March 1, 2017: Journal of Vision
https://www.readbyqxmd.com/read/28153346/usefulness-for-predicting-cardiac-events-after-orthotopic-liver-transplantation-of-myocardial-perfusion-imaging-and-dobutamine-stress-echocardiography-preoperatively
#5
David Snipelisky, Jordan Ray, Saraschandra Vallabhajosyula, Gautam Matcha, Samuel Squier, Jacob Lewis, Rex Holliday, Niti Aggarwal, J Wells Askew, Brian Shapiro, Nandan Anavekar
Patients undergoing orthotopic liver transplantation have high rates of cardiac morbidity and mortality. Although guidelines recommend noninvasive stress testing as part of the preoperative evaluation, little data have evaluated clinical outcomes following orthotopic liver transplantation. A retrospective study at 2 high-volume liver transplantation centers was performed. All patients undergoing noninvasive stress testing (myocardial perfusion imaging [MPI] or dobutamine stress echocardiography [DSE]) over a 5-year period were included...
April 1, 2017: American Journal of Cardiology
https://www.readbyqxmd.com/read/28056735/effect-of-green-tea-phytochemicals-on-mood-and-cognition
#6
Christina Dietz, Matthijs Dekker
BACKGROUND: Green tea is traditionally known to induce mental clarity, cognitive function, physical activation and relaxation. Recently, a special green tea, matcha tea, is rapidly gaining popularity throughout the world and is frequently referred to as a mood- and brain food. Matcha tea consumption leads to much higher intake of green tea phytochemicals compared to regular green tea. Previous research on tea constituents caffeine, L-theanine, and epigallocatechin gallate (EGCG) repeatedly demonstrated benefits on mood and cognitive performance...
January 5, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27867678/reduced-dental-plaque-formation-in-dogs-drinking-a-solution-containing-natural-antimicrobial-herbal-enzymes-and-organic-matcha-green-tea
#7
Michael I Lindinger
The results of an exploratory, multicenter clinical study confirmed the hypothesis that a novel, natural, and safe oral care product (OCP) reduced the rate of plaque formation on teeth of dogs consuming the OCP (antimicrobial plant-derived enzymes, organic matcha green tea, cultured dextrose, sodium bicarbonate, and ascorbic acid) compared to controls. Healthy dogs without periodontitis, of varying breeds, sex, and age, were recruited and enrolled, using nonrandomized stratification methods, into a control and treatment groups...
2016: Scientifica
https://www.readbyqxmd.com/read/27712143/mayo-registry-for-telemetry-efficacy-in-arrest-mr-tea-study-an-assessment-of-the-effect-of-admission-diagnosis-on-outcomes-from-in-hospital-cardiopulmonary-arrest
#8
David Snipelisky, Jordan Ray, Gautam Matcha, Archana Roy, Brooke Clark, Adrian Dumitrascu, Veronica Bosworth, Anastasia Whitman, Patricia Lewis, Tyler Vadeboncoeur, Fred Kusumoto, M Caroline Burton
INTRODUCTION: Little data exists evaluating how different risk factors influence outcomes following in-hospital arrests. METHODS: A retrospective review of patients that suffered a cardiopulmonary arrest between 1 May 2008 and 30 June 2014 was performed. Patients were stratified into subsets based on cardiac versus non-cardiac reasons for admission. RESULTS: 199 patients met inclusion criteria, of which 138 (69.3%) had a non-cardiac reason for admission and 61 (30...
December 2015: Acute Cardiac Care
https://www.readbyqxmd.com/read/27077834/the-powdering-process-with-a-set-of-ceramic-mills-for-green-tea-promoted-catechin-extraction-and-the-ros-inhibition-effect
#9
Kouki Fujioka, Takeo Iwamoto, Hidekazu Shima, Keiko Tomaru, Hideki Saito, Masaki Ohtsuka, Akihiro Yoshidome, Yuri Kawamura, Yoshinobu Manome
For serving green tea, there are two prominent methods: steeping the leaf or the powdered leaf (matcha style) in hot water. The purpose of the present study was to reveal chemical and functional differences before and after the powdering process of green tea leaf, since powdered green tea may contribute to expanding the functionality because of the different ingesting style. In this study, we revealed that the powdering process with a ceramic mill and stirring in hot water increased the average extracted concentration of epigallocatechin gallate (EGCG) by more than three times compared with that in leaf tea using high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass Spectrometry (LC-MS/MS) analyses...
April 11, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27015887/descriptive-analysis-of-medication-administration-during-inpatient-cardiopulmonary-arrest-resuscitation-from-the-mayo-registry-for-telemetry-efficacy-in-arrest-study
#10
David Snipelisky, Jordan Ray, Gautam Matcha, Archana Roy, Adrian Dumitrascu, Dana Harris, Veronica Bosworth, Brooke Clark, Colleen S Thomas, Michael G Heckman, Tyler Vadeboncoeur, Fred Kusumoto, M Caroline Burton
Advanced cardiovascular life support guidelines exist, yet there are variations in clinical practice. Our study aims to describe the utilization of medications during resuscitation from in-hospital cardiopulmonary arrest. A retrospective review of patients who suffered a cardiopulmonary arrest from May 2008 to June 2014 was performed. Clinical and resuscitation data, including timing and dose of medications used, were extracted from the electronic medical record and comparisons made. A total of 94 patients were included in the study...
May 15, 2016: American Journal of Cardiology
https://www.readbyqxmd.com/read/26893319/mayo-registry-for-telemetry-efficacy-in-arrest-study-an-assessment-of-the-utility-of-telemetry-in-predicting-clinical-decompensation
#11
David Snipelisky, Jordan Ray, Gautam Matcha, Archana Roy, Dana Harris, Veronica Bosworth, Adrian Dumitrascu, Brooke Clark, Tyler Vadeboncoeur, Fred Kusumoto, Cammi Bowman, M Caroline Burton
INTRODUCTION: Our study assesses the utility of telemetry in identifying decompensation in patients with documented cardiopulmonary arrest. METHODS: A retrospective review of inpatients who experienced a cardiopulmonary arrest from May 1, 2008, until June 30, 2014, was performed. Telemetry records 24 hours prior to and immediately preceding cardiopulmonary arrest were reviewed. Patient subanalyses based on clinical demographics were made as well as analyses of survival comparing patients with identifiable rhythm changes in telemetry to those without...
February 17, 2016: Journal of Intensive Care Medicine
https://www.readbyqxmd.com/read/26541259/39-year-old-woman-with-headache-and-fever
#12
Gautam V Matcha, Hilary P Steele, Dana M Harris
No abstract text is available yet for this article.
November 2015: Mayo Clinic Proceedings
https://www.readbyqxmd.com/read/26448271/the-effects-of-the-aqueous-extract-and-residue-of-matcha-on-the-antioxidant-status-and-lipid-and-glucose-levels-in-mice-fed-a-high-fat-diet
#13
Ping Xu, Le Ying, Gaojie Hong, Yuefei Wang
Matcha is a kind of powdered green tea produced by grinding with a stone mill. In the present study, the preventive effects of the aqueous extract (water-soluble) and residue (water-insoluble) of Matcha on the antioxidant status and lipid and glucose levels in mice fed a high-fat diet were investigated. Mice were fed seven different experimental diets for 4 weeks: a normal diet control (NC), a high-fat diet (HF), a high-fat diet with 0.025% Matcha (MLD), a high-fat diet with 0.05% Matcha (MMD), a high-fat diet with 0...
January 2016: Food & Function
https://www.readbyqxmd.com/read/26357887/multiresidue-determination-of-pesticides-in-tea-by-gas-chromatography-tandem-mass-spectrometry
#14
Shizuka Saito-Shida, Satoru Nemoto, Reiko Teshima
An efficient and reliable GC-MS/MS method for the multiresidue determination of pesticides in tea was developed by modifying the Japanese official multiresidue method. Sample preparation was carefully optimized for the efficient removal of coextracted matrix components. The optimal sample preparation procedure involved swelling of the sample in water; extraction with acetonitrile; removal of water by salting-out; and sequential cleanup by ODS, graphitized carbon black/primary secondary amine (GCB/PSA) and silica gel cartridges prior to GC-MS/MS analysis...
2015: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/26239615/metal-free-oxidative-c-c-bond-formation-through-c-h-bond-functionalization
#15
Rishikesh Narayan, Kiran Matcha, Andrey P Antonchick
The formation of C-C bonds embodies the core of organic chemistry because of its fundamental application in generation of molecular diversity and complexity. C-C bond-forming reactions are well-known challenges. To achieve this goal through direct functionalization of C-H bonds in both of the coupling partners represents the state-of-the-art in organic synthesis. Oxidative C-C bond formation obviates the need for prefunctionalization of both substrates. This Minireview is dedicated to the field of C-C bond-forming reactions through direct C-H bond functionalization under completely metal-free oxidative conditions...
October 12, 2015: Chemistry: a European Journal
https://www.readbyqxmd.com/read/26160459/cgmd-an-integrated-database-of-cancer-genes-and-markers
#16
Jangampalli Adi Pradeepkiran, Sri Bhashyam Sainath, Konidala Kramthi Kumar, Lokanada Balasubramanyam, Kodali Vidya Prabhakar, Matcha Bhaskar
Integrating cancer genes and markers with experimental evidence might provide valuable information for the further investigation of crosstalk between tumor genes and markers in cancer biology. To achieve this objective, we developed a database known as the Cancer Gene Marker Database (CGMD), which integrates data on tumor genes and markers based on experimental evidence. The major goal of CGMD is to provide the following: 1) current systematic treatment approaches and recent advances in different cancer treatments; 2) the aggregation of different genes and markers by their molecular characteristics and pathway associations; and 3) free access to the data compiled by CGMD at http://cgmd...
2015: Scientific Reports
https://www.readbyqxmd.com/read/26004048/design-synthesis-in-silico-and-in-vitro-studies-of-substituted-1-2-3-4-tetrahydro-pyrimidine-phosphorus-derivatives
#17
Kilaru Ravendra Babu, Yellapu Nanda Kumar, Aminedi Raghavendra, Venukadasula Phanindra, Golla Madhava, Nuchu Ravi, Matcha Bhaskar, Chamarthi Naga Raju
Molecular docking studies of the designed two series (4a-l, 6a-l, 9 and 10) of novel substituted phosphorylated 1, 4-dihydropyridine and 1,2,3,4-tetrahydropyrimidine derivatives against the drug targets of DHFR from Bacillus cereus, LpxC from Pseudomonas aeruginosa, IDH from E. coli and MurB from Staphylococcus aureus were encouraged for their synthesis. These compounds were synthesized from substituted aromatic aldehydes, thiourea/urea and ethyl acetoacetate in the presence of polyphosphoric acid (PPA). These were further phosphorylated with diethyl (2-chloroethoxy) methyl phosphonate to get the desired products...
2015: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/25891959/mayo-registry-for-telemetry-efficacy-in-arrest-mr-tea-study-an-analysis-of-code-status-change-following-cardiopulmonary-arrest
#18
David Snipelisky, Jordan Ray, Gautam Matcha, Archana Roy, Razvan Chirila, Michael Maniaci, Veronica Bosworth, Anastasia Whitman, Patricia Lewis, Tyler Vadeboncoeur, Fred Kusumoto, M Caroline Burton
INTRODUCTION: Code status discussions are important during a hospitalization, yet variation in its practice exists. No data have assessed the likelihood of patients to change code status following a cardiopulmonary arrest. METHODS: A retrospective review of all patients that experienced a cardiopulmonary arrest between May 1, 2008 and June 30, 2014 at an academic medical center was performed. The proportion of code status modifications to do not resuscitate (DNR) from full code was assessed...
July 2015: Resuscitation
https://www.readbyqxmd.com/read/25848225/modeling-molecular-dynamics-and-docking-assessment-of-transcription-factor-rho-a-potential-drug-target-in-brucella-melitensis-16m
#19
Jangampalli Adi Pradeepkiran, Konidala Kranthi Kumar, Yellapu Nanda Kumar, Matcha Bhaskar
The zoonotic disease brucellosis, a chronic condition in humans affecting renal and cardiac systems and causing osteoarthritis, is caused by Brucella, a genus of Gram-negative, facultative, intracellular pathogens. The mode of transmission and the virulence of the pathogens are still enigmatic. Transcription regulatory elements, such as rho proteins, play an important role in the termination of transcription and/or the selection of genes in Brucella. Adverse effects of the transcription inhibitors play a key role in the non-successive transcription challenges faced by the pathogens...
2015: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/25834405/complete-genome-wide-screening-and-subtractive-genomic-approach-revealed-new-virulence-factors-potential-drug-targets-against-bio-war-pathogen-brucella-melitensis-16m
#20
Jangampalli Adi Pradeepkiran, Sri Bhashyam Sainath, Konidala Kranthi Kumar, Matcha Bhaskar
Brucella melitensis 16M is a Gram-negative coccobacillus that infects both animals and humans. It causes a disease known as brucellosis, which is characterized by acute febrile illness in humans and causes abortions in livestock. To prevent and control brucellosis, identification of putative drug targets is crucial. The present study aimed to identify drug targets in B. melitensis 16M by using a subtractive genomic approach. We used available database repositories (Database of Essential Genes, Kyoto Encyclopedia of Genes and Genomes Automatic Annotation Server, and Kyoto Encyclopedia of Genes and Genomes) to identify putative genes that are nonhomologous to humans and essential for pathogen B...
2015: Drug Design, Development and Therapy
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