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https://www.readbyqxmd.com/read/28905094/recent-advances-on-the-difructose-anhydride-iv-preparation-from-levan-conversion
#1
REVIEW
Hua Hang
Difructose anhydride IV (DFA IV) is a cyclic disaccharide consisting of two fructose residues, which is obtained from levan conversion with levan fructotransferase (LFTase) and rarely found in nature as a low-calorie sugar substitute. Some beneficial effects of DFA IV connected with its consumption have been described. The article reviews the properties and physiological functions of DFA IV, levan conversion, resources and properties of LFTase and the produced methods of DFA IV. LFTase as a relatively novel enzyme and its molecular evolution are discussed as well...
September 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28871528/co-production-of-fructooligosaccharides-and-levan-by-levansucrase-from-bacillus-subtilis-natto-with-potential-application-in-the-food-industry
#2
Gabrielly Terassi Bersaneti, Nicole Caldas Pan, Cristiani Baldo, Maria Antonia Pedrine Colabone Celligoi
Fructooligosaccharides and levan have a wide range of applications in the food industry due to their physiological and functional properties. The enzymatic synthesis of these molecules exhibits great advantages when compared with microbial fermentation. In this study, the production of levansucrase from Bacillus subtilis natto and its utilization in fructooligosaccharides and levan syntheses using different reaction conditions were described. The best condition for levansucrase production was 420.7 g L(-1) of sucrose at pH 7...
September 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28831661/bariatric-surgery-is-gaining-ground-as-treatment-of-obesity-after-heart-transplantation-report-of-two-cases
#3
Levan Tsamalaidze, Enrique F Elli
Experience with bariatric surgery in patients after orthotopic heart transplantation (OHT) is still limited. We performed a retrospective review of patients who underwent bariatric surgery after OHT from January 1, 2010 to December 31, 2016. Two post-OHT patients with BMI of 37.5 and 36.2 kg/m² underwent laparoscopic robotic-assisted Roux-en-Y gastric bypass and laparoscopic sleeve gastrectomy, respectively. Quality of life substantially improved for both patients. Bariatric surgery is safe and feasible in OHT patients, despite numerous risk factors...
August 22, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28803941/fast-imaging-for-mapping-dynamic-networks
#4
REVIEW
Pierre LeVan, Burak Akin, Jürgen Hennig
The development of highly accelerated fMRI acquisition techniques has led to novel possibilities to monitor cerebral activity non-invasively and with unprecedented temporal resolutions. With the emergence of dynamic connectivity and its ability to provide a much richer characterization of brain function compared to static measures, fast fMRI may yet play a crucial role in tracking dynamically varying networks. In spite of the dominance of slow hemodynamic contributions to the BOLD signal, high temporal sampling rates nevertheless improve the measurement of physiological noise, yielding an exceptional sensitivity for the detection of periods of transient connectivity at time scales of a few tens of seconds...
August 10, 2017: NeuroImage
https://www.readbyqxmd.com/read/28802013/effects-of-levan-type-fructan-on-growth-performance-nutrient-digestibility-diarrhea-scores-fecal-shedding-of-total-lactic-acid-bacteria-and-coliform-bacteria-and-fecal-gas-emission-in-weaning-pigs
#5
Xin Jian Lei, Yong Min Kim, Jae Hong Park, Dong Heon Baek, Charles Martin Nyachoti, In Ho Kim
BACKGROUND: The use of antibiotics as growth promoters in feed has been fully or partially banned in several countries. The objective of this study was to evaluate effects of levan-type fructan on growth performance, nutrient digestibility, fecal shedding of lactic acid bacteria and coliform bacteria, diarrhea scores, and fecal gas emission in weaning pigs. A total of 144 weaning pigs [(Yorkshire × Landrace) × Duroc] were randomly allocated to 4 diets: corn-soybean meal-based diets supplemented with 0, 0...
August 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28799827/laparoscopic-splenectomy-for-massive-splenomegaly-does-size-matter
#6
Levan Tsamalaidze, John A Stauffer, Samantha L Permenter, Horacio J Asbun
BACKGROUND: Laparoscopic splenectomy (LS) has become the most common approach for elective splenectomy, but use of LS for patients with massive splenomegaly (MS) remains controversial. By the 2008 European guidelines, LS for MS (spleen size >20 cm) is generally not recommended. METHODS: We performed a retrospective analysis of 229 consecutive patients undergoing LS, hand-assist (HALS), and open splenectomy (OS) at our institution from January 1, 1995 to December 2016...
August 11, 2017: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/28766265/laparoscopic-sleeve-gastrectomy-for-morbid-obesity-in-patients-after-orthotopic-liver-transplant-a-matched-case-control-study
#7
Levan Tsamalaidze, John A Stauffer, Lisa C Arasi, Diego E Villacreses, Jose Salvador Serrano Franco, Steven Bowers, Enrique F Elli
INTRODUCTION: Obesity is frequently encountered in patients with orthotopic liver transplant (OLT). The role of bariatric surgery is still unclear for this specific population. The aim of this study was to review our experience with laparoscopic sleeve gastrectomy (LSG) after OLT. MATERIAL AND METHODS: We performed a retrospective case-control study of patients undergoing LSG after OLT from 2010 to 2016. OLT-LSG patients were matched by age, sex, body mass index (BMI), and year to non-OLT patients undergoing LSG...
August 1, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28746029/early-tissue-damage-and-microstructural-reorganization-predict-disease-severity-in-experimental-epilepsy
#8
Philipp Janz, Niels Schwaderlapp, Katharina Heining, Ute Häussler, Jan G Korvink, Dominik von Elverfeldt, Jürgen Hennig, Ulrich Egert, Pierre LeVan, Carola A Haas
Mesial temporal lobe epilepsy (mTLE) is the most common focal epilepsy in adults and is often refractory to medication. So far, resection of the epileptogenic focus represents the only curative therapy. It is unknown whether pathological processes preceding epilepsy onset are indicators of later disease severity. Using longitudinal multi-modal MRI, we monitored hippocampal injury and tissue reorganization during epileptogenesis in a mouse mTLE model. The prognostic value of MRI biomarkers was assessed by retrospective correlations with pathological hallmarks Here, we show for the first time that the extent of early hippocampal neurodegeneration and progressive microstructural changes in the dentate gyrus translate to the severity of hippocampal sclerosis and seizure burden in chronic epilepsy...
July 26, 2017: ELife
https://www.readbyqxmd.com/read/28732036/an-accurate-coarse-grained-model-for-chitosan-polysaccharides-in-aqueous-solution
#9
Levan Tsereteli, Andrea Grafmüller
Computational models can provide detailed information about molecular conformations and interactions in solution, which is currently inaccessible by other means in many cases. Here we describe an efficient and precise coarse-grained model for long polysaccharides in aqueous solution at different physico-chemical conditions such as pH and ionic strength. The Model is carefully constructed based on all-atom simulations of small saccharides and metadynamics sampling of the dihedral angles in the glycosidic links, which represent the most flexible degrees of freedom of the polysaccharides...
2017: PloS One
https://www.readbyqxmd.com/read/28717996/patterns-of-oribatid-mite-species-diversity-testing-the-effects-of-elevation-area-and-sampling-effort
#10
Levan Mumladze, Maka Murvanidze, Mark Maraun
Elevational gradients in species diversity and species area relationships are two well established patterns that are not mutually exclusive in space and time. Elevation and area are both considered as good proxies to detect and characterize the patterns of species diversity distribution. However, such studies are hampered by the incomplete biodiversity data available for ecologists, which may affect the pattern perceptions. Using the large dataset of oribatid mite communities sampled in Georgia, we tested the effects of altitude and area on species distribution using various approaches, while explicitly considering the biases from sampling effort...
July 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28714842/molecular-and-biochemical-characteristics-of-the-inulosucrase-hugo-from-streptomyces-viridochromogenes-dsm40736-t%C3%A3-494
#11
Hans-Jörg Frasch, Sander S van Leeuwen, Lubbert Dijkhuizen
Polyfructans are synthesized from sucrose by plants (mostly inulin) and by both Gram-negative and Gram-positive bacteria (mostly levan). In the phylum Actinobacteria only levan synthesis by Actinomyces species has been reported. We have identified a putative fructansucrase gene (hugO) in Streptomyces viridochromogenes DSM40736 (Tü494). HugO was heterologously expressed and biochemically characterized. HPSEC-MALLS and 2D-1H-13C nuclear magnetic resonance (NMR) spectroscopy analysis showed that the fructan polymer produced in vitro has an Molecular Weight of 2...
July 17, 2017: Microbiology
https://www.readbyqxmd.com/read/28637539/laparoscopic-excision-of-remnant-gallbladder-after-subtotal-cholecystectomy
#12
Levan Tsamalaidze, Tariq Almerey, Lucio Pereira, Samantha Permenter, John A Stauffer
No abstract text is available yet for this article.
June 1, 2017: American Surgeon
https://www.readbyqxmd.com/read/28635269/reduction-potentials-of-fefe-hydrogenase-accessory-iron-sulfur-clusters-provide-insights-into-the-energetics-of-proton-reduction-catalysis
#13
Jacob H Artz, David W Mulder, Michael W Ratzloff, Carolyn E Lubner, Oleg A Zadvornyy, Axl X LeVan, S Garrett Williams, Michael W W Adams, Anne K Jones, Paul W King, John W Peters
An [FeFe]-hydrogenase from Clostridium pasteurianum, CpI, is a model system for biological H2 activation. In addition to the catalytic H-cluster, CpI contains four accessory iron-sulfur [FeS] clusters in a branched series that transfer electrons to and from the active site. In this work, potentiometric titrations have been employed in combination with electron paramagnetic resonance (EPR) spectroscopy at defined electrochemical potentials to gain insights into the role of the accessory clusters in catalysis...
July 6, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28602989/brachybacterium-sp-ch-kov3-isolated-from-an-oil-polluted-environment-a-new-producer-of-levan
#14
Aleksandra Djurić, Gordana Gojgić-Cvijović, Dragica Jakovljević, Branka Kekez, Jovana Stefanović Kojić, Maija-Liisa Mattinen, Inka Elina Harju, Miroslav M Vrvić, Vladimir P Beškoski
Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced...
November 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28595337/response-to-the-association-of-progressive-tension-sutures-and-drains-seems-to-decrease-the-drainage-output-and-seroma-formation-in-abdominoplasty
#15
Samer Jabbour, Rani Makhoul, Cyril Awaida, Philippe Levan, Taliah Schmitt, Marwan Nasr
No abstract text is available yet for this article.
June 8, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28588563/insight-into-potential-probiotic-markers-predicted-in-lactobacillus-pentosus-mp-10-genome-sequence
#16
Hikmate Abriouel, Beatriz Pérez Montoro, Carlos S Casimiro-Soriguer, Antonio J Pérez Pulido, Charles W Knapp, Natacha Caballero Gómez, Sonia Castillo-Gutiérrez, María D Estudillo-Martínez, Antonio Gálvez, Nabil Benomar
Lactobacillus pentosus MP-10 is a potential probiotic lactic acid bacterium originally isolated from naturally fermented Aloreña green table olives. The entire genome sequence was annotated to in silico analyze the molecular mechanisms involved in the adaptation of L. pentosus MP-10 to the human gastrointestinal tract (GIT), such as carbohydrate metabolism (related with prebiotic utilization) and the proteins involved in bacteria-host interactions. We predicted an arsenal of genes coding for carbohydrate-modifying enzymes to modify oligo- and polysaccharides, such as glycoside hydrolases, glycoside transferases, and isomerases, and other enzymes involved in complex carbohydrate metabolism especially starch, raffinose, and levan...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28575294/impaired-coordination-of-nucleophile-and-increased-hydrophobicity-in-the-1-subsite-shift-levansucrase-activity-towards-transfructosylation
#17
Maria Elena Ortiz-Soto, Christian Possiel, Julian Görl, Andreas Vogel, Ramona Schmiedel, Jürgen Seibel
Bacterial levansucrases produce β(2,6)-linked levan-type polysaccharides using sucrose or sucrose analogues as donor/acceptor substrates. However, the dominant reaction of Bacillus megaterium levansucrase (Bm-LS) is hydrolysis. Single domain levansucrases from Gram-positive bacteria display a wide substrate-binding pocket with open access to water, challenging engineering for transfructosylation-efficient enzymes. We pursued a shift in reaction specificity by either modifying the water distribution in the active site or the coordination of the catalytic acid/base (E352) and the nucleophile (D95), thus affecting the fructosyl-transfer rate and allowing acceptors other than water to occupy the active site...
May 30, 2017: Glycobiology
https://www.readbyqxmd.com/read/28554522/engineering-aspects-of-microbial-exopolysaccharide-production
#18
Filomena Freitas, Cristiana A V Torres, Maria A M Reis
Although the ability to secrete exopolysaccharides (EPS) is widespread among microorganisms, only a few bacterial (e.g. xanthan, levan, dextran) and fungal (e.g. pullulan) EPS have reached full commercialization. During the last years, other microbial EPS producers have been the subject of extensive research, including endophytes, extremophiles, microalgae and Cyanobacteria, as well as mixed microbial consortia. Those studies have demonstrated the great potential of such microbial systems to generate biopolymers with novel chemical structures and distinctive functional properties...
May 18, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28535808/effect-of-bacteria-type-and-sucrose-concentration-on-levan-yield-and-its-molecular-weight
#19
Álvaro González-Garcinuño, Antonio Tabernero, José Mª Sánchez-Álvarez, Miguel A Galán, Eva M Martin Del Valle
BACKGROUND: Levan has been traditionally produced from microorganism. However, there is a continuous effort in looking for new strains that improve levan production yield and uses alternative sugar sources for growth. Despite having a wide range of data about levan yield, there are not papers which allow controlling molecular weight, and that plays an essential role for further applications. RESULTS: The effect of the sucrose concentration on levan yield (and its molecular weight) from Bacillus atrophaeus and Acinetobacter nectaris (Gram positive and Gram negative respectively) was studied in this work...
May 23, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28522999/fermentation-ph-modulates-the-size-distributions-and-functional-properties-of-gluconobacter-albidus-tmw-2-1191-levan
#20
Tharalinee Ua-Arak, Frank Jakob, Rudi F Vogel
Bacterial levan has gained an increasing interest over the last decades due to its unique characteristics and multiple possible applications. Levan and other exopolysaccharides (EPSs) production are usually optimized to obtain the highest concentration or yield while a possible change of the molecular size and mass during the production process is mostly neglected. In this study, the molar mass and radius of levan samples were monitored during fermentations with the food-grade, levan-producing acetic acid bacterium Gluconobacter (G...
2017: Frontiers in Microbiology
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