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https://www.readbyqxmd.com/read/28522999/fermentation-ph-modulates-the-size-distributions-and-functional-properties-of-gluconobacter-albidus-tmw-2-1191-levan
#1
Tharalinee Ua-Arak, Frank Jakob, Rudi F Vogel
Bacterial levan has gained an increasing interest over the last decades due to its unique characteristics and multiple possible applications. Levan and other exopolysaccharides (EPSs) production are usually optimized to obtain the highest concentration or yield while a possible change of the molecular size and mass during the production process is mostly neglected. In this study, the molar mass and radius of levan samples were monitored during fermentations with the food-grade, levan-producing acetic acid bacterium Gluconobacter (G...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28464843/immunohistochemical-evaluation-of-epithelial-ovarian-carcinomas-identifies-three-different-expression-patterns-of-the-mx35-antigen-napi2b
#2
Kristina Levan, Matin Mehryar, Constantina Mateoiu, Per Albertsson, Tom Bäck, Karin Sundfeldt
BACKGROUND: To characterize the expression of the membrane transporter NaPi2b and antigen targeted by the MX35 antibody in ovarian tumor samples. The current interest to develop monoclonal antibody based therapy of ovarian cancer by targeting NaPi2b emphasizes the need for detailed knowledge and characterization of the expression pattern of this protein. For the majority of patients with ovarian carcinoma the risk of being diagnosed in late stages with extensive loco-regional spread disease is substantial, which stresses the need to develop improved therapeutic agents...
May 2, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28449124/labia-majora-augmentation-a-systematic-review-of-the-literature
#3
Samer Jabbour, Elio Kechichian, Barbara Hersant, Philippe Levan, Lena El Hachem, Warren Noel, Marwan Nasr
Background: Currently, there is no standardized approach for labia majora augmentation and controversies still exist regarding this subject. Objectives: This systematic review aimed to assess the evidence in the literature regarding labia majora augmentation. Methods: On November 20, 2016, we conducted an online search of published articles in the Medline, Embase, and Cochrane databases. All articles describing labia majora augmentation were included in this review...
April 26, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28421913/type-vi-secretion-systems-of-erwinia-amylovora-contribute-to-bacterial-competition-virulence-and-exopolysaccharide-production
#4
Yanli Tian, Yuqiang Zhao, Linye Shi, Zhongli Cui, Baishi Hu, Youfu Zhao
The type VI secretion system (T6SS) plays a major role in mediating interbacterial competition and might contribute to virulence in plant pathogenic bacteria. However, the role of T6SS in Erwinia amylovora remains unknown. In this study, 33 deletion mutants within three T6SS clusters were generated in E. amylovora strain NCPPB1665. Our results showed that all 33 mutants displayed reduced antibacterial activities against Escherichia coli as compared with that of the wild-type (WT) strain, indicating that Erwinia amylovora T6SS are functional...
June 2017: Phytopathology
https://www.readbyqxmd.com/read/28412340/levan-promotes-antiproliferative-and-pro-apoptotic-effects-in-mcf-7-breast-cancer-cells-mediated-by-oxidative-stress
#5
Eveline A I F Queiroz, Zuleica B Fortes, Mário A A da Cunha, Hande Kazak Sarilmiser, Aneli M Barbosa Dekker, Ebru Toksoy Öner, Robert F H Dekker, Neelam Khaper
Exopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of levan (a (2→6)-β-d-fructan) produced on sucrose by the halophilic bacterium, Halomonas smyrnensis AAD6(T), in human breast cancer MCF-7 cells. MCF-7 cells were exposed to levan for 24 and 48h...
April 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28400025/influence-of-levan-producing-acetic-acid-bacteria-on-buckwheat-sourdough-breads
#6
Tharalinee Ua-Arak, Frank Jakob, Rudi F Vogel
Buckwheat sourdoughs supplemented with molasses as natural sucrose source were fermented with levan-producing Gluconobacter (G.) albidus TMW 2.1191 and Kozakia (K.) baliensis NBRC 16680. Cell growth, concomitant levan and low-molecular-weight metabolite production were monitored. Sourdough breads were prepared with different sourdoughs from both strains (24, 30 and 48 h fermentation, respectively) and analyzed with respect to bread volume, crumb hardness and sensory characteristics. During fermentation, levan, acetic and gluconic acids were increasingly produced, while spontaneously co-growing lactic acid bacteria additionally formed acetic and lactic acids...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28398515/gluteal-implants-versus-autologous-flaps-in-patients-with-postbariatric-surgery-weight-loss-a-prospective-comparative-study-of-3-dimensional-gluteal-projection-after-lower-body-lift
#7
Philippe Levan, Samer Bassilios Habre
Background: Deformities and excess skin resulting from massive weight loss are corrected with circumferential lower body lift (LBL). The gluteal area is frequently flattened due to aggressive skin excision during LBL. Gluteal implants can circumvent this problem. Objectives: We aimed to objectively evaluate the outcomes of gluteal augmentation with implants versus flap surgery performed simultaneously with LBL. Methods: Between January 2014 and June 2015, twenty-seven patients underwent LBL with gluteal implants (10 patients), flaps (14 patients), or no gluteal augmentation (3 patients) in our hospital...
April 7, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28363576/novel-levan-and-pnipa-temperature-sensitive-hydrogels-for-5-asa-controlled-release
#8
Asila Osman, Ebru Toksoy Oner, Mehmet S Eroglu
Levan based cross-linker was successfully synthesized and used to prepare a series of more biocompatible and temperature responsive levan/N-isopropyl acrylamide (levan/pNIPA) hydrogels by redox polymerization at room temperature. Volume phase transition temperature (VPTT) of the hydrogels were precisely determined by derivative differential scanning calorimetry (DDSC). Incorporation of levan into the pNIPA hydrogel increased the VPTT from 32.8°C to 35.09°C, approaching to body temperature. Swelling behavior and 5-aminosalicylic acid (5-ASA) release of the hydrogels were found to vary significantly with temperature and composition...
June 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28350252/long-term-cognitive-and-neuroanatomical-stability-in-patients-with-anoxic-amnesia-a-case-report
#9
Kacie L Wright, C Brock Kirwan, Shawn D Gale, Ashley J Levan, Ramona O Hopkins
BACKGROUND: Anoxia can result in selective hippocampal damage with associated impairments in declarative memory. Whilst memory impairments and brain structures are thought to be stable, there are little data regarding the effects of ageing or change over time in patients with amnesia from anoxic brain injury. METHODS: To assess change over time, we compared structural magnetic resonance imaging (MRI) with data obtained over ten years previously in two well-characterized patients with amnesia (JRW and RS) who experienced an anoxic brain injury...
March 28, 2017: Brain Injury: [BI]
https://www.readbyqxmd.com/read/28302109/interaction-of-cd14-haplotypes-and-soluble-cd14-on-pulmonary-function-in-agricultural-workers
#10
Tricia D LeVan, Lynette M Smith, Art J Heires, Ted R Mikuls, Jane L Meza, Lisa A Weissenburger-Moser, Debra J Romberger
BACKGROUND: Agricultural environments are contaminated with organic dusts containing bacterial components. Chronic inhalation of organic dusts is implicated in respiratory diseases. CD14 is a critical receptor for gram-negative lipopolysaccharide; however, its association with respiratory disease among agricultural workers is unknown. The objective of this study was to determine if serum soluble CD14 (sCD14) levels are associated with lung function among agricultural workers and if this association is modified by genetic variants in CD14...
March 16, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28274423/a-novel-two-step-enzymatic-synthesis-of-blastose-a-%C3%AE-d-fructofuranosyl-2%C3%A2-6-d-glucopyranose-sucrose-analogue
#11
Alfonso Miranda-Molina, Edmundo Castillo, Agustin Lopez Munguia
Blastose, a natural disaccharide found in honey, is usually found as a byproduct of fructo-oligosaccharide synthesis from sucrose with fructosyltransferases. In this study, we describe a novel two-step biosynthetic route to obtain blastose, designed from a detailed observation of B. subtilis levansucrase (SacB) acceptor structural requirements for fructosylation. The strategy consisted first in the synthesis of the trisaccharide O-β-d-Fruf-(2↔6)-O-α-d-Glcp-(1↔1)-α-d-Glcp, through a regioselective β-d-transfructosylation of trehalose (Tre) which acts as acceptor in a reaction catalyzed by SacB using sucrose or levan as fructosyl donor...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28271385/possible-correlation-between-levansucrase-production-and-probiotic-activity-of-bacillus-sp-isolated-from-honey-and-honey-bee
#12
Abdelhamid A Hamdy, Nouran A Elattal, Magdy A Amin, Amal E Ali, Nahla M Mansour, Ghada E A Awad, Hassan M Awad, Mona A Esawy
Five bacterial isolates from honey and bee gut were selected based on their high levansucrase activity and levan yield which were strongly positively correlated. All isolates showed good tolerance to temperature up to 70 °C, to NaCl up to 3 M and to 0.1% H2O2. They maintained over 59 and 64% survival at pH 9.0 and 2.0 respectively, but showed varying tolerance to 0.1% bile salts and pancreatic enzymes. Most isolates were susceptible to widely used antibiotics, but demonstrated diverse antimicrobial activity...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28197690/improvement-of-levan-production-in-bacillus-amyloliquefaciens-through-metabolic-optimization-of-regulatory-elements
#13
Yanyan Gu, Jiayi Zheng, Jun Feng, Mingfeng Cao, Weixia Gao, Yufen Quan, Yulei Dang, Yi Wang, Shufang Wang, Cunjiang Song
Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous promoters were evaluated for sacB expression, and the Pgrac promoter led to the highest level for both sacB transcription and levansucrase enzyme activity. The levan production in the corresponding recombinant strain ΔLP-pHTPgrac reached 30...
February 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28123183/epistatic-effect-of-tlr-1-6-and-10-polymorphisms-on-organic-dust-mediated-cytokine-response
#14
L M Smith, L A Weissenburger-Moser, A J Heires, K L Bailey, D J Romberger, T D LeVan
Exposure to organic dust from agricultural environments is associated with inflammatory respiratory conditions. The putative causal agents in organic dust include viral, microbial and fungal components, which are recognized by the family of Toll-like receptors (TLRs) and drive host innate and adaptive responses. Our aim in this study was to determine whether responsiveness to organic dust among agricultural workers was dependent on polymorphisms in the TLR10-TLR1-TLR6 gene cluster. We stimulated whole blood from 509 agricultural workers with organic dust, triacyl lipopeptide N-palmitoyl-S-dipalmitoylglyceryl Cys-Ser-(Lys)4 (Pam3CSK4) and the diacyl-lipopeptide peptidoglycan...
March 2017: Genes and Immunity
https://www.readbyqxmd.com/read/28115645/evaluating-the-toxicity-of-cigarette-whole-smoke-solutions-in-an-air-liquid-interface-human-in-vitro-airway-tissue-model
#15
Xuefei Cao, Levan Muskhelishvili, John Latendresse, Patricia Richter, Robert H Heflich
Exposure to cigarette smoke causes a multitude of pathological changes leading to tissue damage and disease. Quantifying such changes in highly differentiated in vitro human tissue models may assist in evaluating the toxicity of tobacco products. In this methods development study, well-differentiated human air-liquid-interface (ALI) in vitro airway tissue models were used to assess toxicological endpoints relevant to tobacco smoke exposure. Whole mainstream smoke solutions (WSSs) were prepared from 2 commercial cigarettes (R60 and S60) that differ in smoke constituents when machine-smoked under International Organization for Standardization conditions...
March 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28115097/development-of-a-nanoparticle-system-based-on-a-fructose-polymer-stability-and-drug-release-studies
#16
Antonio Tabernero, Álvaro González-Garcinuño, José Mª Sánchez-Álvarez, Miguel A Galán, Eva M Martín Del Valle
New drug delivery systems (DDSs) with levan or its carboxymethylated form, as carriers, and 5-fluorouracil as a drug, are produced in this work. Levan is obtained after cultivating A. nectaris and polymer nanoparticles are created in water by a self-assembled process. The effect of pH and the ionic strength on polymer nanoparticles aggregation is studied. Basic pHs produces a particle size between 300 and 400nm with a Z-potential around -20mV because a basic medium promotes repulsion forces. DDSs of 300-400nm and a Z-potential about -25mV are prepared by taking advantage of the amphiphilic properties of the levan...
March 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28103254/a-highly-active-endo-levanase-bt1760-of-a-dominant-mammalian-gut-commensal-bacteroides-thetaiotaomicron-cleaves-not-only-various-bacterial-levans-but-also-levan-of-timothy-grass
#17
Karin Mardo, Triinu Visnapuu, Heiki Vija, Anneli Aasamets, Katrin Viigand, Tiina Alamäe
Bacteroides thetaiotaomicron, an abundant commensal of the human gut, degrades numerous complex carbohydrates. Recently, it was reported to grow on a β-2,6-linked polyfructan levan produced by Zymomonas mobilis degrading the polymer into fructooligosaccharides (FOS) with a cell surface bound endo-levanase BT1760. The FOS are consumed by B. thetaiotaomicron, but also by other gut bacteria, including health-promoting bifidobacteria and lactobacilli. Here we characterize biochemical properties of BT1760, including the activity of BT1760 on six bacterial levans synthesized by the levansucrase Lsc3 of Pseudomonas syringae pv...
2017: PloS One
https://www.readbyqxmd.com/read/28075130/expression-and-characterization-of-levansucrase-from-clostridium-acetobutylicum
#18
Song Gao, Xianghui Qi, Darren J Hart, Herui Gao, Yingfeng An
The Clostridium acetobutylicum gene Ca-SacB encoding levansucrase was cloned and expressed in Escherichia coli. Ca-SacB is composed of 1287 bp and encodes 428 amino acid residues, which could convert 150 mmol/L sucrose to levan with the liberation of glucose. The optimum pH and temperature of this enzyme for levan formation were pH 6 and 60 °C, respectively. Levansucrase activity of Ca-SacB was completely abolished by 5 mmol/L Ag(+) and Hg(2+). The Km and Vmax values for levansucrase were calculated to be 64 mmol/L and 190 μmol/min/mg, respectively...
February 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28017156/sugar-based-biopolymers-in-nanomedicine-new-emerging-era-for-cancer-imaging-and-therapy
#19
Mehmet S Eroglu, Ebru Toksoy Oner, Esra Cansever Mutlu, Muge Sennaroglu Bostan
Since last decade, sugar based biopolymers are recognized in nanomedicine as promising materials for cancer imaging and therapy. Their durable, biocompatible and adhesive properties enable the fine tuning of their molecular weights (MW) and their miscellaneous nature makes the molecules acquire various conformations. These in turn provide effective endocytosis by cancer cell membranes that have already been programmed for internalization of different kinds of sugars. Therefore, biocompatible sugar based nanoparticles (SBNPs) are suitable for both cell-selective delivery of drugs and imaging through the human body...
December 21, 2016: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28004643/electrically-driven-magnetic-antenna-based-on-multiferroic-composites
#20
X-G Wang, A Sukhov, L Chotorlishvili, C-L Jia, G-H Guo, J Berakdar
We suggest and demonstrate via large scale numerical simulations an electrically operated spin-wave inducer based on composite multiferroic junctions. Specifically, we consider an interfacially coupled ferromagnetic/ferroelectric structure that emits controllably spin waves in the ferromagnets if the ferroelectric polarization is poled by an external electric field. The roles of geometry and material properties are discussed.
March 8, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
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