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https://www.readbyqxmd.com/read/29157907/expression-and-purification-of-plant-fructan-exohydrolases-and-their-potential-applications-in-fructose-production
#1
Wenyue Zhan, Lijin Jin, Jiao Jiao, Xi Zhang, Yan Zhang, Haiyan Zhao, Mingxiang Liang
Inulinases from microorganisms have been extensively studied for their role in the production of fructose from fructan. Fructan can also be hydrolyzed by plant fructan exohydrolases (FEHs), but these enzymes have not been used to produce fructose commercially. Two Ht1-FEHs (Ht1-FEH I and Ht1-FEH II) were recently characterized in Jerusalem artichoke. In this study, we cloned the third member of the Ht1-FEH family in Jerusalem artichoke (i.e., Ht1-FEH III). When heterologously expressed in Pichia pastoris X-33, Ht1-FEH III not only demonstrated hydrolysis activity towards β (2, 1)-linked fructans and β (2, 6)-linked levan, but also towards sucrose...
November 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29148362/pseudomonas-floridensis-sp-nov-a-bacterial-pathogen-isolated-from-tomato
#2
Sujan Timilsina, Gerald V Minsavage, James Preston, Eric A Newberry, Matthews L Paret, Erica M Goss, Jeffrey B Jones, Gary E Vallad
An unusual fluorescent pseudomonad was isolated from tomato exhibiting leaf spot symptoms similar to bacterial speck. Strains were fluorescent, oxidase- and arginine-dihydrolase-negative, elicited a hypersensitive reaction on tobacco and produced a soft rot on potato slices. However, the strains produced an unusual yellow, mucoid growth on media containing 5 % sucrose that is not typical of levan. Based on multilocus sequence analysis using 16S rRNA, gap1, gltA, gyrB and rpoD, these strains formed a distinct phylogenetic group in the genus Pseudomonas and were most closely related to Pseudomonas viridiflava within the Pseudomonassyringae complex...
November 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29135892/-lower-body-lift-in-the-massive-weight-loss-patient-a-new-classification-and-algorithm-for-gluteal-augmentation
#3
Taliah Schmitt, Samer Jabbour, Rani Makhoul, Warren Noel, Anne-Sophie Reguesse, Philippe Levan
BACKGROUND: An often neglected part of the lower body lift procedure is the gluteal region. The objective of this study is to classify the massive weight loss (MWL) patients undergoing a body lift procedure and provide a safe, standardized approach for gluteal augmentation. METHODS: A retrospective review of all body lift procedures performed between January 2012 and January 2017 was conducted. Patients undergoing a lower body lift with or without gluteal augmentation were included for analysis...
November 8, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29135536/investigating-uranium-isotopic-ratios-and-activity-ratios-in-groundwaters-in-the-state-of-idaho
#4
Levan Tkavadze, Richard R Brey, John Dudgeon
Analyzing uranium isotopic and activity ratios can give valuable information for hydrologic and environmental studies such as insights to weathering processes, estimating water mixing ratios, and identifying water sources. The authors employed an inductively coupled plasma mass spectrometer (ICP-MS) to perform environmental level concentration measurements of isotopic uranium on 380 groundwater samples from various locations within the state of Idaho. The U:U uranium activity ratios (UAR) for these samples range between 0...
November 10, 2017: Health Physics
https://www.readbyqxmd.com/read/29133100/physicochemical-properties-of-a-high-molecular-weight-levan-from-brenneria-sp-enid312
#5
Wei Xu, Qian Liu, Yuxiang Bai, Shuhuai Yu, Tao Zhang, Bo Jiang, Wanmeng Mu
A high molecular weight levan was produced by a novel levansucrase and some properties of this polymer were investigated. The levan exhibited a poroid microstructure as well as series of individual ellipsoidal or spheroidal particles. The weight-average molecular weight (M¯w) of the levan was determined to be 1.41×10(8)Da. In a 0.1% solution, the levan showed a mean diameter of 176nm, while in a 1% solution the diameter was 182nm. The decomposition temperature was determined to be 216.67°C, with an endothermic peak at 147...
November 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29132806/heart-transplantation-in-an-era-of-mechanical-circulatory-support
#6
REVIEW
Jenny Kwak, Michael Majewski, Pierre T LeVan
No abstract text is available yet for this article.
September 28, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/29117186/human-papillomavirus-type-16-e6-and-nfx1-123-mislocalize-immune-signaling-proteins-and-downregulate-immune-gene-expression-in-keratinocytes
#7
Justine Levan, Portia A Vliet-Gregg, Kristin L Robinson, Rachel A Katzenellenbogen
Human papillomavirus (HPV) is the most prevalent sexually transmitted infection, affecting an estimated 11% of the world's population. The high-risk HPV types (HR HPV) account for approximately 5% of the global burden of cancer and thus cause high morbidity and mortality. Although it is known that persistent infection with HR HPV is the greatest risk factor for developing HPV-associated cancer, and that the HPV early proteins E6 and E7 dysregulate immune detection by its host cells, the mechanisms of immune evasion by HR HPV are not well understood...
2017: PloS One
https://www.readbyqxmd.com/read/29103838/cognitive-and-behavioral-comorbidities-in-rolandic-epilepsy-and-their-relation-with-default-mode-network-s-functional-connectivity-and-organization
#8
Isabell Ofer, Julia Jacobs, Nathalie Jaiser, Burak Akin, Jürgen Hennig, Andreas Schulze-Bonhage, Pierre LeVan
OBJECTIVE: Rolandic epilepsy (RE) is characterized by typical interictal-electroencephalogram (EEG) patterns mainly localized in centrotemporal and parietooccipital areas. An aberrant intrinsic organization of the default mode network (DMN) due to repeated disturbances from spike-generating areas may be able to account for specific cognitive deficits and behavioral problems in RE. The aim of the present study was to investigate cognitive development (CD) and socioemotional development (SED) in patients with RE during active disease in relation to DMN connectivity and network topology...
November 2, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/29094694/spectroscopic-identification-of-r-process-nucleosynthesis-in-a-double-neutron-star-merger
#9
E Pian, P D'Avanzo, S Benetti, M Branchesi, E Brocato, S Campana, E Cappellaro, S Covino, V D'Elia, J P U Fynbo, F Getman, G Ghirlanda, G Ghisellini, A Grado, G Greco, J Hjorth, C Kouveliotou, A Levan, L Limatola, D Malesani, P A Mazzali, A Melandri, P Møller, L Nicastro, E Palazzi, S Piranomonte, A Rossi, O S Salafia, J Selsing, G Stratta, M Tanaka, N R Tanvir, L Tomasella, D Watson, S Yang, L Amati, L A Antonelli, S Ascenzi, M G Bernardini, M Boër, F Bufano, A Bulgarelli, M Capaccioli, P Casella, A J Castro-Tirado, E Chassande-Mottin, R Ciolfi, C M Copperwheat, M Dadina, G De Cesare, A Di Paola, Y Z Fan, B Gendre, G Giuffrida, A Giunta, L K Hunt, G L Israel, Z-P Jin, M M Kasliwal, S Klose, M Lisi, F Longo, E Maiorano, M Mapelli, N Masetti, L Nava, B Patricelli, D Perley, A Pescalli, T Piran, A Possenti, L Pulone, M Razzano, R Salvaterra, P Schipani, M Spera, A Stamerra, L Stella, G Tagliaferri, V Testa, E Troja, M Turatto, S D Vergani, D Vergani
The merger of two neutron stars is predicted to give rise to three major detectable phenomena: a short burst of γ-rays, a gravitational-wave signal, and a transient optical-near-infrared source powered by the synthesis of large amounts of very heavy elements via rapid neutron capture (the r-process). Such transients, named 'macronovae' or 'kilonovae', are believed to be centres of production of rare elements such as gold and platinum. The most compelling evidence so far for a kilonova was a very faint near-infrared rebrightening in the afterglow of a short γ-ray burst at redshift z = 0...
November 2, 2017: Nature
https://www.readbyqxmd.com/read/29080817/production-and-structural-characterization-of-exopolysaccharides-from-newly-isolated-probiotic-lactic-acid-bacteria
#10
Yousra Abid, Angela Casillo, Houda Gharsallah, Ichrak Joulak, Rosa Lanzetta, Maria Michela Corsaro, Hamadi Attia, Samia Azabou
In this work, four exopolysaccharide-producing lactic acid bacteria (LAB) strains, newly isolated from Tunisian spontaneously fermented foods and beverages, namely bovine and turkey meat sausages (BMS and TMS), date palm sap (DPS) and cow milk (CM), were identified as Leuconostoc citreum-BMS, Leuconostoc mesenteroides-TMS, Pediococcus pentosaceus-DPS and Leuconostoc pseudomesenteroides-CM, respectively. The isolated strains showed the ability to withstand simulated human gastrointestinal (GI) tract conditions (low pH, lysozyme, bile salts, pepsin and pancreatin) and showed high surface hydrophobicity (79-90%), besides their ability to act against Escherichia coli and Listeria monocytogenes and to produce exopolysaccharides (EPS)...
October 25, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29055698/formation-of-new-cytocompatible-hydrogels-based-on-photochemically-crosslinkable-levan-methacrylates
#11
Albrecht Berg, Ebru Toksoy Oner, Joan Combie, Bernd Schneider, Renate Ellinger, Jürgen Weisser, Ralf Wyrwa, Matthias Schnabelrauch
Levan is a high molecular weight fructose-based biotechnologically available polysaccharide with a range of interesting properties qualifying this molecule for applications in biomedicine. In this study, new levan derivatives containing methacrylate groups attached either via ester or urethane linkages to the fructan backbone could be synthesized and structurally characterized by conventional analytical techniques. The photochemical crosslinking of these substances applying different photo-initiator systems and reaction conditions resulted in hydrogels of diverse properties which were investigated with regard to mechanical behaviour, hydrolytic degradability, and cytocompatibility...
October 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29038371/swift-and-nustar-observations-of-gw170817-detection-of-a-blue-kilonova
#12
P A Evans, S B Cenko, J A Kennea, S W K Emery, N P M Kuin, O Korobkin, R T Wollaeger, C L Fryer, K K Madsen, F A Harrison, Y Xu, E Nakar, K Hotokezaka, A Lien, S Campana, S R Oates, E Troja, A A Breeveld, F E Marshall, S D Barthelmy, A P Beardmore, D N Burrows, G Cusumano, A D'Aì, P D'Avanzo, V D'Elia, M de Pasquale, W P Even, C J Fontes, K Forster, J Garcia, P Giommi, B Grefenstette, C Gronwall, D H Hartmann, M Heida, A L Hungerford, M M Kasliwal, H A Krimm, A J Levan, D Malesani, A Melandri, H Miyasaka, J A Nousek, P T O'Brien, J P Osborne, C Pagani, K L Page, D M Palmer, M Perri, S Pike, J L Racusin, S Rosswog, M H Siegel, T Sakamoto, B Sbarufatti, G Tagliaferri, N R Tanvir, A Tohuvavohu
With the first direct detection of merging black holes in 2015, the era of gravitational wave (GW) astrophysics began. A complete picture of compact object mergers, however, requires the detection of an electromagnetic (EM) counterpart. We report ultraviolet (UV) and x-ray observations by Swift and the Nuclear Spectroscopic Telescope ARray (NuSTAR) of the EM counterpart of the binary neutron star merger GW 170817. The bright, rapidly fading ultraviolet emission indicates a high mass (≈ 0.03 solar masses) wind-driven outflow with moderate electron fraction (Ye ≈ 0...
October 16, 2017: Science
https://www.readbyqxmd.com/read/29022389/the-composition-and-metabolism-of-faecal-microbiota-is-specifically-modulated-by-different-dietary-polysaccharides-and-mucin-an-isothermal-microcalorimetry-study
#13
K Adamberg, K Kolk, M Jaagura, R Vilu, S Adamberg
The metabolic activity of colon microbiota is specifically affected by fibres with various monomer compositions, degree of polymerisation and branching. The supply of a variety of dietary fibres assures the diversity of gut microbial communities considered important for the well-being of the host. The aim of this study was to compare the impact of different oligo- and polysaccharides (galacto- and fructooligosaccharides, resistant starch, levan, inulin, arabinogalactan, xylan, pectin and chitin), and a glycoprotein mucin on the growth and metabolism of faecal microbiota in vitro by using isothermal microcalorimetry (IMC)...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28962785/levan-type-fructooligosaccharides-synthesis-by-a-levansucrase-endolevanase-fusion-enzyme-levb1sacb
#14
Jaime R Porras-Domínguez, María Elena Rodríguez-Alegría, Ángela Ávila-Fernández, Silvia Montiel-Salgado, Agustín López-Munguía
We describe here the enzymatic production of levan type-fructooligosaccharides (L-FOS) with a DP from 2 to 10, through simultaneous synthesis and hydrolysis reactions. This was accomplished by LevB1SacB, a new enzyme resulting from the fusion of SacB, a levansucrase from Bacillus subtilis and LevB1, an endolevanase from B. licheniformis. In the fusion enzyme, SacB retains its catalytic behavior with a decrease in kcat from 164 to 108s(-1). LevB1 in LevB1SacB kinetic behavior improves considerably reaching saturation with levan and following Michaelis-Menten kinetics, quite differently from the previously reported first order kinetic behavior...
December 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28958997/effects-of-difructose-dianhydride-dfa-iv-on-in-vitro-fertilization-in-pigs
#15
Young-Joo Yi, S Kamala-Kannan, Jeong-Muk Lim, Byung-Taek Oh, Sang-Myeong Lee
Difructose dianhydride IV (DFA-IV) is produced from levan, which is a natural polysaccharide that belongs to the fructan family, through the activity of levan fructotransferase (LF) derived from microorganisms. Recently, DFA-IV has been expected to have diverse applications in the food and medical industry. Here, we examined the potential application of DFA-IV forin vitro fertilization (IVF) in pigs. In the assessment of acrosomal integrity during incubation, intact acrosomal or viable spermatozoa were highly sustained in 0...
September 26, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28905094/recent-advances-on-the-difructose-anhydride-iv-preparation-from-levan-conversion
#16
REVIEW
Hua Hang
Difructose anhydride IV (DFA IV) is a cyclic disaccharide consisting of two fructose residues, which is obtained from levan conversion with levan fructotransferase (LFTase) and rarely found in nature as a low-calorie sugar substitute. Some beneficial effects of DFA IV connected with its consumption have been described. The article reviews the properties and physiological functions of DFA IV, levan conversion, resources and properties of LFTase and the produced methods of DFA IV. LFTase as a relatively novel enzyme and its molecular evolution are discussed as well...
October 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28871528/co-production-of-fructooligosaccharides-and-levan-by-levansucrase-from-bacillus-subtilis-natto-with-potential-application-in-the-food-industry
#17
Gabrielly Terassi Bersaneti, Nicole Caldas Pan, Cristiani Baldo, Maria Antonia Pedrine Colabone Celligoi
Fructooligosaccharides and levan have a wide range of applications in the food industry due to their physiological and functional properties. The enzymatic synthesis of these molecules exhibits great advantages when compared with microbial fermentation. In this study, the production of levansucrase from Bacillus subtilis natto and its utilization in fructooligosaccharides and levan syntheses using different reaction conditions were described. The best condition for levansucrase production was 420.7 g L(-1) of sucrose at pH 7...
September 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28831661/bariatric-surgery-is-gaining-ground-as-treatment-of-obesity-after-heart-transplantation-report-of-two-cases
#18
Levan Tsamalaidze, Enrique F Elli
Experience with bariatric surgery in patients after orthotopic heart transplantation (OHT) is still limited. We performed a retrospective review of patients who underwent bariatric surgery after OHT from January 1, 2010 to December 31, 2016. Two post-OHT patients with BMI of 37.5 and 36.2 kg/m² underwent laparoscopic robotic-assisted Roux-en-Y gastric bypass and laparoscopic sleeve gastrectomy, respectively. Quality of life substantially improved for both patients. Bariatric surgery is safe and feasible in OHT patients, despite numerous risk factors...
November 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28803941/fast-imaging-for-mapping-dynamic-networks
#19
REVIEW
Pierre LeVan, Burak Akin, Jürgen Hennig
The development of highly accelerated fMRI acquisition techniques has led to novel possibilities to monitor cerebral activity non-invasively and with unprecedented temporal resolutions. With the emergence of dynamic connectivity and its ability to provide a much richer characterization of brain function compared to static measures, fast fMRI may yet play a crucial role in tracking dynamically varying networks. In spite of the dominance of slow hemodynamic contributions to the BOLD signal, high temporal sampling rates nevertheless improve the measurement of physiological noise, yielding an exceptional sensitivity for the detection of periods of transient connectivity at time scales of a few tens of seconds...
August 10, 2017: NeuroImage
https://www.readbyqxmd.com/read/28802013/effects-of-levan-type-fructan-on-growth-performance-nutrient-digestibility-diarrhea-scores-fecal-shedding-of-total-lactic-acid-bacteria-and-coliform-bacteria-and-fecal-gas-emission-in-weaning-pigs
#20
Xin Jian Lei, Yong Min Kim, Jae Hong Park, Dong Heon Baek, Charles Martin Nyachoti, In Ho Kim
BACKGROUND: The use of antibiotics as growth promoters in feed has been fully or partially banned in several countries. The objective of this study was to evaluate effects of levan-type fructan on growth performance, nutrient digestibility, fecal shedding of lactic acid bacteria and coliform bacteria, diarrhea scores, and fecal gas emission in weaning pigs. A total of 144 weaning pigs [(Yorkshire × Landrace) × Duroc] were randomly allocated to 4 diets: corn-soybean meal-based diets supplemented with 0, 0...
August 12, 2017: Journal of the Science of Food and Agriculture
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