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hydratation, body mass

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https://www.readbyqxmd.com/read/28330086/cloning-expression-purification-and-characterization-of-human-mitochondrial-carbonic-anhydrase-va
#1
Danish Idrees, Sudhir Kumar, Syed Abdul Arif Rehman, Samudrala Gourinath, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3(-) and proton. CAV is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion. Here, we report a new strategy for cloning, expression and purification for CAVA in the bacterial system followed by its biophysical characterization. The cDNA of CAVA, a 801 nucleotide long that encodes a 267-amino acid polypeptide of molecular mass of 30-kDa (excluding signal peptide), was sub-cloned in the expression vector pET21c and transformed into Escherichia coli strain BL21 (DE3) for expression...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28319679/evaluation-of-body-composition-with-bioimpedence-a-comparison-between-athletic-and-non-athletic-children
#2
Domenico Meleleo, Nicola Bartolomeo, Liliana Cassano, Alessandro Nitti, Giovanna Susca, Giuseppina Mastrototaro, Umberto Armenise, Annapaola Zito, Fiorella Devito, Pietro Scicchitano, Marco Matteo Ciccone
PURPOSE: Conventional Bioelectrical Impedance Analysis (BIA) or Bioelectrical Impedance Vector Analysis (BIVA) can provide direct evaluations of body composition. The purpose of this study was to evaluate lean and fat mass (FM), and hydration of children involved in daily competitive sports. METHODS: 190 non-athletic [8.2-10.5 years] and 29 competitive children [8.0-10.5 years] were enrolled. They were evaluated: at baseline (t0), 6 months (t1) and one year (t2)...
March 20, 2017: European Journal of Sport Science
https://www.readbyqxmd.com/read/28315876/angiotensin-converting-enzyme-ins-del-polymorphism-and-body-composition-the-intermediary-role-of-hydration-status
#3
Laura Bordoni, Valerio Napolioni, Francesca Marchegiani, Emilio Amadio, Rosita Gabbianelli
BACKGROUND: The well-known insertion/deletion polymorphism (rs4646994) of the angiotensin-converting enzyme (ACE) gene has been previously associated with obesity, blood flow, muscular strength, and ACE enzyme activity. Despite the relevant role of ACE in homeostasis, few data are currently available on the relationship between rs4646994 and hydration status. Thus, we tested the association between the ACE Ins/Del polymorphism, body composition, and hydration status in a young Italian population...
March 18, 2017: Journal of Nutrigenetics and Nutrigenomics
https://www.readbyqxmd.com/read/28272337/impact-of-isotonic-beverage-on-the-hydration-status-of-healthy-chinese-adults-in-air-conditioned-environment
#4
Phei Ching Siow, Wei Shuan Kimberly Tan, Christiani Jeyakumar Henry
People living in tropical climates spend much of their time in confined air-conditioned spaces, performing normal daily activities. This study investigated the effect of distilled water (W) or isotonic beverage (IB) on the hydration status in subjects living under these conditions. In a randomized crossover design, forty-nine healthy male subjects either consumed beverage or IB over a period of 8 h (8 h) in a controlled air-conditioned environment. Blood, urine, and saliva samples were collected at baseline and after 8 h...
March 7, 2017: Nutrients
https://www.readbyqxmd.com/read/28255913/body-composition-anthropometric-indices-and-hydration-status-of-obstructive-sleep-apnea-patients-can-cachexia-coexist-with-obesity
#5
Barbara Kuźnar-Kamińska, Marcin Grabicki, Tomasz Trafas, Monika Szulińska, Szczepan Cofta, Tomasz Piorunek, Beata Brajer-Luftmann, Agata Nowicka, Barbara Bromińska, Halina Batura-Gabryel
The aim of this study was to elucidate body composition, anthropometric indices, and hydration status in obstructive sleep apnea (OSA) patients, taking into account different disease stages, gender, and the possibility of the presence of cachexia. There were 98 OSA patients and 23 control subjects enrolled into the study. All study participants underwent polysomnography examination. Body mass index (BMI), fat mass index (FMI), fat free mass, muscle mass, body cell mass, total body water, and extracellular and intracellular water were evaluated...
March 3, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28240714/effects-of-exercise-induced-dehydration-and-glycerol-rehydration-on-anaerobic-power-in-male-collegiate-wrestlers
#6
Zachary J McKenna, Trevor L Gillum
Wrestlers attempting to reach a specific weight class often use rapid weight loss (RWL). RWL is associated with high levels of dehydration, which may hinder athletic performance. Thus, there is a need for wrestlers to optimize re-hydration after achieving a specific weight. We sought to observe the effects of RWL on anaerobic power and the impact of glycerol on rehydration and power in male collegiate wrestlers (n=7, 19.75 ± 1.67 years, 76.8 ± 4.32 kg, 11.6 ± 4.32 % body fat, 59.9 ± 6.42 ml/kg/min). Subjects were assessed for body mass (BM), hydration, and mean power output (Wmean) before exercise (pre), immediately post (3% dehydrated), and 60 min post (rehydrated)...
February 23, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28204901/the-effect-of-hydration-status-on-the-measurement-of-lean-tissue-mass-by-dual-energy-x-ray-absorptiometry
#7
Clodagh M Toomey, William G McCormack, Phil Jakeman
PURPOSE: Athletes cycle between exercise and recovery. Exercise invokes changes in total body water from thermal sweating, muscle and hepatic glycogen depletion and metabolic water loss. Recovery from exercise results in rehydration, substrate repletion, and possible glycogen supercompensation. Such changes may corrupt the measurement of hydrated tissues, such as lean tissue mass (LTM), by dual-energy X-ray absorptiometry (DXA). The purpose of this study was to determine the effect of exercise and thermal dehydration and subsequent glycogen supercompensation on DXA-based measurement of body composition...
March 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28203205/intraocular-pressure-is-a-poor-predictor-of-hydration-status-following-intermittent-exercise-in-the-heat
#8
Ian B Stewart, Brittany Dias, David N Borg, Aaron J E Bach, Beatrix Feigl, Joseph T Costello
Current hydration assessments involve biological fluids that are either compromised in dehydrated individuals or require laboratory equipment, making timely results unfeasible. The eye has been proposed as a potential site to provide a field-based hydration measure. The present study evaluated the efficacy and sensitivity of intraocular pressure (IOP) to assess hydration status. Twelve healthy males undertook two 150 min walking trials in 40°C 20% relative humidity. One trial matched fluid intake to body mass loss (control, CON) and the other had fluid restricted (dehydrated, DEH)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28165555/nutritional-status-and-intrarenal-arterial-stiffness-in-cardiorenal-syndrome-a-pilot-study
#9
A Gigante, F Di Mario, B Barbano, E Rosato, G Di Lazzaro Giraldi, R Pofi, M L Gasperini, D Amoroso, R Cianci, A Laviano
OBJECTIVE: Cardio-Renal Syndrome (CRS) is a condition, which is more frequently observed in clinical practice. The aim of this study is to explore nutritional status and intrarenal arterial stiffness in patients affected by CRS. PATIENTS AND METHODS: 14 consecutive CRS patients, screened for anthropometry, biochemistry, nutritional and metabolic status underwent renal Doppler ultrasound and whole-body bioimpedance spectroscopy (BIS). RESULTS: We found a positive correlation between phase angle (PA) and CKD-EPI and MDRD (p=0...
January 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28149344/prevalence-of-dehydration-before-training-sessions-friendly-and-official-matches-in-elite-female-soccer-players
#10
Mauricio Castro-Sepulveda, Javier Astudillo, Pablo Letelier, Hermann Zbinden-Foncea
This study aimed to evaluate and compare the hydration states prior to different sporting events (training sessions, friendly and official matches) in elite female soccer players and relate that to the importance that the player attached to the hydration state as a determinant of sports performance. The hydration state of 17 female elite soccer players (age: 21.5 ± 3 years; body mass: 62 ± 6 kg; body height: 165 ± 9 cm) was determined by measuring their urine specific gravity (USG) prior to three different sports events: training sessions (PT), friendly (PF) and official (PO) matches...
April 1, 2016: Journal of Human Kinetics
https://www.readbyqxmd.com/read/28145415/changes-in-serum-osmotic-pressure-following-haemodialysis-treatments-lead-to-changes-in-bioimpedance-spectroscopy-estimates-of-lean-and-adipose-tissue
#11
M Antonelou, S El-Kateb, N Davies, A Davenport
Haemodialysis (HD) patients are at risk of sarcopenia. Newer bioimpedance devices (BIS) using a three-compartmental body composition model, separate extracellular water overhydration from normo-hydrated lean tissue mass (LTM) and adipose tissue mass (ATM). During HD hydration status changes, along with changes in electrolytes and solutes, and may alter body composition measurements. As such, we measured BIS and serum osmotic pressure (sOP) pre- and post dialysis in 43 patients. There were no significant changes in LTM (39...
February 1, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28129234/inflammation-relates-to-resistance-training-induced-hypertrophy-in-elderly-patients
#12
Kristoffer Larsen Norheim, Christopher Kjaer Cullum, Jesper Loevind Andersen, Michael Kjaer, Anders Karlsen
PURPOSE: Aging is associated with a gradual loss of muscle mass, which some have suggested to be accelerated by short periods of muscle disuse due to medical illness. We investigated the effect of hospitalization on skeletal muscle mass in acutely ill geriatric patients with focus on the relationship between systemic inflammatory marker C-reactive protein (CRP) and changes in muscle mass, as well as the influence of resistance training upon muscle mass. METHOD: Unilateral leg press resistance exercise was conducted daily during the hospital period...
January 26, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28122662/validity-of-bioelectrical-impedance-analysis-in-estimation-of-fat-free-mass-in-colorectal-cancer-patients
#13
Hanna Ræder, Ane Sørlie Kværner, Christine Henriksen, Geir Florholmen, Hege Berg Henriksen, Siv Kjølsrud Bøhn, Ingvild Paur, Sigbjørn Smeland, Rune Blomhoff
BACKGROUND & AIMS: Bioelectrical impedance analysis (BIA) is an accessible and cheap method to measure fat-free mass (FFM). However, BIA estimates are subject to uncertainty in patient populations with altered body composition and hydration. The aim of the current study was to validate a whole-body and a segmental BIA device against dual-energy X-ray absorptiometry (DXA) in colorectal cancer (CRC) patients, and to investigate the ability of different empiric equations for BIA to predict DXA FFM (FFMDXA)...
January 12, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28112821/a-pilot-study-on-the-correlation-between-fat-fraction-values-and-glucose-uptake-values-in-supraclavicular-fat-by-simultaneous-pet-mri
#14
Andrew McCallister, Le Zhang, Alex Burant, Laurence Katz, Rosa Tamara Branca
PURPOSE: To assess the spatial correlation between MRI and 18F-fludeoxyglucose positron emission tomography (FDG-PET) maps of human brown adipose tissue (BAT) and to measure differences in fat fraction (FF) between glucose avid and non-avid regions of the supraclavicular fat depot using a hybrid FDG-PET/MR scanner. METHODS: In 16 healthy volunteers, mean age of 30 and body mass index of 26, FF, R2*, and FDG uptake maps were acquired simultaneously using a hybrid PET/MR system while employing an individualized cooling protocol to maximally stimulate BAT...
January 23, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28112767/nutritional-status-and-habitual-dietary-intake-are-associated-with-frail-skin-conditions-in-community-dwelling-older-people
#15
S Iizaka, S Nagata, H Sanada
OBJECTIVE: Prevention of frail skin is important in older people because frail skin is associated with a risk of injury in this population. In this study, we investigated the association of nutritional status and habitual dietary intake with skin conditions in community-dwelling older people. DESIGN: Cross-sectional study. SETTING: Three community settings in Japan from autumn to winter. PARTICIPANTS: Older people aged ≥65 years without care-need certification (n=118)...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28079435/bioelectrical-impedance-analysis-of-head-and-neck-cancer-patients-at-presentation
#16
Marie Lundberg, Pia Nikander, Katja Tuomainen, Helena Orell-Kotikangas, Antti Mäkitie
CONCLUSION: Finnish head and neck cancer (HNC) patients show signs of severe malnutrition already at presentation, measured by bioelectric impedance analysis (BIA). BIA may be a practical method to detect malnutrition, analyze body composition, and to identify high-risk patients in this population. OBJECTIVES: BIA is a validated method for evaluating body composition and detecting malnutrition. Low phase angle (PA) is associated with increased mortality and morbidity...
January 12, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28009789/heat-strain-is-exacerbated-on-the-second-of-consecutive-days-of-fire-suppression
#17
Zachary J Schlader, Deanna Colburn, David Hostler
PURPOSE: We tested the hypothesis that physiological and perceptual heat strain is exacerbated on the second of back-to-back days of fire suppression work despite evidence of full recovery. METHODS: Twenty-six career and volunteer firefighters (age: 31± 8 y) completed 20 min of near maximal fire suppression work on consecutive days. Dependent variables were core temperature, heart rate, perceived exertion and thermal sensation, which were measured before and after fire suppression...
December 22, 2016: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27990382/general-measures-and-quality-of-life-issues-in-psoriasis
#18
Rashmi Sarkar, Shikha Chugh, Shivani Bansal
Psoriasis generally does not affect survival but has significant detrimental effect on quality of life (QOL), which may be comparable to that of ischemic heart disease, diabetes, depression, and cancer. The foremost important thing in the management of psoriasis is counseling of the patient. The clinician needs to be empathetic and spend adequate time with the patient and educating the patient about psoriasis. Clinicians should make it clear to the patient that the primary goal of treatment is control of the disease rather than cure...
November 2016: Indian Dermatology Online Journal
https://www.readbyqxmd.com/read/27935519/the-role-of-obesity-in-the-relation-between-total-water-intake-and-urine-osmolality-in-us-adults-2009-2012
#19
Asher Y Rosinger, Hannah G Lawman, Lara J Akinbami, Cynthia L Ogden
BACKGROUND: Adequate water intake is critical to physiologic and cognitive functioning. Although water requirements increase with body size, it remains unclear whether weight status modifies the relation between water intake and hydration status. OBJECTIVE: We examined how the association between water intake and urine osmolality, which is a hydration biomarker, varied by weight status. DESIGN: NHANES cross-sectional data (2009-2012) were analyzed in 9601 nonpregnant adults aged ≥20 y who did not have kidney failure...
December 2016: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/27932993/effect-of-permissive-dehydration-on-induction-and-decay-of-heat-acclimation-and-temperate-exercise-performance
#20
Rebecca A Neal, Heather C Massey, Michael J Tipton, John S Young, Jo Corbett
Purpose: It has been suggested that dehydration is an independent stimulus for heat acclimation (HA), possibly through influencing fluid-regulation mechanisms and increasing plasma volume (PV) expansion. There is also some evidence that HA may be ergogenic in temperate conditions and that this may be linked to PV expansion. We investigated: (i) the influence of dehydration on the time-course of acquisition and decay of HA; (ii) whether dehydration augmented any ergogenic benefits in temperate conditions, particularly those related to PV expansion...
2016: Frontiers in Physiology
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