keyword
https://read.qxmd.com/read/38486280/rubisco-activity-assays-a-simplified-biochemical-redox-approach-for-in-vitro-quantification-and-an-rna-sensor-approach-for-in-vivo-monitoring
#1
JOURNAL ARTICLE
Muhammad Faisal, Aditya P Sarnaik, Nandini Kannoju, Nima Hajinajaf, Muhammad Javaid Asad, Ryan W Davis, Arul M Varman
BACKGROUND: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant soluble protein in nature. Extensive studies have been conducted for improving its activity in photosynthesis through approaches like protein engineering. Concurrently, multiple biochemical and radiolabeling assays have been developed for determining its activity. Although these existing assays yield reliable results, they require addition of multiple external components, rendering them less convenient and expensive...
March 14, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38482749/aspirin-mediated-acetylation-of-sirt1-maintains-intestinal-immune-homeostasis
#2
JOURNAL ARTICLE
Liangguo Xie, Chaoqun Li, Chao Wang, Zhen Wu, Changchun Wang, Chunyu Chen, Xiaojian Chen, Dejian Zhou, Qiang Zhou, Ping Lu, Chen Ding, Chen-Ying Liu, Jinzhong Lin, Xumin Zhang, Xiaofei Yu, Wei Yu
Aspirin, also named acetylsalicylate, can directly acetylate the side-chain of lysine in protein, which leads to the possibility of unexplained drug effects. Here, the study used isotopic-labeling aspirin-d3 with mass spectrometry analysis to discover that aspirin directly acetylates 10 HDACs proteins, including SIRT1, the most studied NAD+ -dependent deacetylase. SIRT1 is also acetylated by aspirin in vitro. It is also identified that aspirin directly acetylates lysine 408 of SIRT1, which abolishes SIRT1 deacetylation activity by impairing the substrates binding affinity...
March 14, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/38481042/dual-treatment-with-kynurenine-pathway-inhibitors-and-nad-precursors-synergistically-extends-life-span-in-drosophila
#3
JOURNAL ARTICLE
Mariann M Gabrawy, Reyhan Westbrook, Austin King, Nick Khosravian, Neeraj Ochaney, Tagide DeCarvalho, Qinchuan Wang, Yuqiong Yu, Qiao Huang, Adam Said, Michael Abadir, Cissy Zhang, Pratik Khare, Jennifer E Fairman, Anne Le, Ginger L Milne, Fernando J Vonhoff, Jeremy D Walston, Peter M Abadir
Tryptophan catabolism is highly conserved and generates important bioactive metabolites, including kynurenines, and in some animals, NAD+ . Aging and inflammation are associated with increased levels of kynurenine pathway (KP) metabolites and depleted NAD+ , factors which are implicated as contributors to frailty and morbidity. Contrastingly, KP suppression and NAD+ supplementation are associated with increased life span in some animals. Here, we used DGRP_229 Drosophila to elucidate the effects of KP elevation, KP suppression, and NAD+ supplementation on physical performance and survivorship...
March 13, 2024: Aging Cell
https://read.qxmd.com/read/38474420/nicotinamide-riboside-augments-human-macrophage-migration-via-sirt3-mediated-prostaglandin-e2-signaling
#4
JOURNAL ARTICLE
Jing Wu, Maximilian Bley, Russell S Steans, Allison M Meadows, Rebecca D Huffstutler, Rong Tian, Julian L Griffin, Michael N Sack
NAD+ boosting via nicotinamide riboside (NR) confers anti-inflammatory effects. However, its underlying mechanisms and therapeutic potential remain incompletely defined. Here, we showed that NR increased the expression of CC-chemokine receptor 7 (CCR7) in human M1 macrophages by flow cytometric analysis of cell surface receptors. Consequently, chemokine ligand 19 (CCL19, ligand for CCR7)-induced macrophage migration was enhanced following NR administration. Metabolomics analysis revealed that prostaglandin E2 (PGE2) was increased by NR in human monocytes and in human serum following in vivo NR supplementation...
March 5, 2024: Cells
https://read.qxmd.com/read/38464264/nicotinamide-riboside-activates-renal-metabolism-and-protects-the-kidney-in-a-model-of-alport-syndrome
#5
Bryce A Jones, Debora L Gisch, Komuraiah Myakala, Amber Sadiq, Ying-Hua Cheng, Elizaveta Taranenko, Julia Panov, Kyle Korolowicz, Xiaoxin Wang, Avi Z Rosenberg, Sanjay Jain, Michael T Eadon, Moshe Levi
Chronic kidney disease (CKD) is associated with renal metabolic disturbances, including impaired fatty acid oxidation (FAO). Nicotinamide adenine dinucleotide (NAD + ) is a small molecule that participates in hundreds of metabolism-related reactions. NAD + levels are decreased in CKD, and NAD + supplementation is protective. However, both the mechanism of how NAD + supplementation protects from CKD, as well as the cell types most responsible, are poorly understood. Using a mouse model of Alport syndrome, we show that nicotinamide riboside (NR), an NAD + precursor, stimulates renal peroxisome proliferator-activated receptor α signaling and restores FAO in the proximal tubules, thereby protecting from CKD in both sexes...
February 29, 2024: bioRxiv
https://read.qxmd.com/read/38463014/nampt-nad-parp1-pathway-regulates-cfa-induced-inflammatory-pain-via-nf-%C3%AE%C2%BAb-signaling-in-rodents
#6
JOURNAL ARTICLE
Yi Dai, Jiaqi Lin, Xiangde Chen, Jinxuan Ren, Chengwei Wu, Huihui Shen, Xue Li, Jing Yu, Baochun Jiang, Lina Yu
Emerging evidence has implicated nicotinamide adenine dinucleotide (NAD+ ) metabolism in various inflammatory diseases. In the study, the role of NAD+ metabolism in Complete Freund's Adjuvant (CFA)-evoked inflammatory pain and the underlying mechanisms are investigated. The study demonstrated that CFA induced upregulation of nicotinamide phosphoribosyltransferase (NAMPT) in dorsal root ganglia (DRG) without significant changes in the spinal cord. Inhibition of NAMPT expression by intrathecal injection of NAMPT siRNA alleviated CFA-induced pain-like behavior, decreased NAD+ contents in DRG, and lowered poly-(ADP-ribose) polymerase 1 (PARP1) activity levels...
March 11, 2024: Advanced biology
https://read.qxmd.com/read/38459256/macrod1-suppresses-diabetic-cardiomyopathy-via-regulating-parp1-nad-sirt3-pathway
#7
JOURNAL ARTICLE
Yu-Ting Liu, Hong-Liang Qiu, Hong-Xia Xia, Yi-Zhou Feng, Jiang-Yang Deng, Yuan Yuan, Da Ke, Heng Zhou, Yan Che, Qi-Zhu Tang
Diabetic cardiomyopathy (DCM), one of the most serious long-term consequences of diabetes, is closely associated with oxidative stress, inflammation and apoptosis in the heart. MACRO domain containing 1 (Macrod1) is an ADP-ribosylhydrolase 1 that is highly enriched in mitochondria, participating in the pathogenesis of cardiovascular diseases. In this study, we investigated the role of Macrod1 in DCM. A mice model was established by feeding a high-fat diet (HFD) and intraperitoneal injection of streptozotocin (STZ)...
March 8, 2024: Acta Pharmacologica Sinica
https://read.qxmd.com/read/38452174/differentially-disrupted-spinal-cord-and-muscle-energy-metabolism-in-spinal-and-bulbar-muscular-atrophy
#8
JOURNAL ARTICLE
Danielle DeBartolo, Frederick J Arnold, Yuhong Liu, Elana Molotsky, Hsin-Yao Tang, Diane E Merry
Prior studies showed that polyQ-expanded AR is aberrantly acetylated and that deacetylation of the mutant AR by overexpression of NAD+-dependent sirtuin 1 (SIRT1) is protective in cell models of spinal and bulbar muscular atrophy (SBMA). Based on these observations and reduced NAD+ in muscles of SBMA mouse models, we tested the therapeutic potential of NAD+ restoration in vivo by treating post-symptomatic transgenic SBMA mice with the nicotinamide adenine dinucleotide (NAD+) precursor nicotinamide riboside (NR)...
March 5, 2024: JCI Insight
https://read.qxmd.com/read/38435305/untargeted-metabolomics-unveiled-the-role-of-butanoate-metabolism-in-the-development-of-pseudomonas-aeruginosa-hypoxic-biofilm
#9
JOURNAL ARTICLE
Ahmed G Abdelhamid, Ahmed E Yousef
Pseudomonas aeruginosa is a versatile opportunistic pathogen which causes a variety of acute and chronic human infections, some of which are associated with the biofilm phenotype of the pathogen. We hypothesize that defining the intracellular metabolome of biofilm cells, compared to that of planktonic cells, will elucidate the metabolic pathways and biomarkers indicative of biofilm inception. Disc-shaped stainless-steel coupons (12.7 mm diameter) were employed as a surface for static biofilm establishment. Each disc was immersed in a well, of a 24-well microtiter plate, containing a 1-mL Lysogeny broth (LB) suspension of P...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38430946/towards-personalized-nicotinamide-mononucleotide-nmn-supplementation-nicotinamide-adenine-dinucleotide-nad-concentration
#10
JOURNAL ARTICLE
Ajla Hodzic Kuerec, Weilan Wang, Lin Yi, Rongsheng Tao, Zhigang Lin, Aditi Vaidya, Sohal Pendse, Sornaraja Thasma, Niranjan Andhalkar, Ganesh Avhad, Vidyadhar Kumbhar, Andrea B Maier
Nicotinamide mononucleotide (NMN) is a precursor of nicotinamide adenine dinucleotide (NAD), which declines with age. Supplementation of NMN has been shown to improve blood NAD concentration. However, the optimal NMN dose remains unclear. This is a post-hoc analysis of a double-blinded clinical trial involving 80 generally healthy adults aged 40-65 years. The participants received a placebo or daily 300 mg, 600 mg, or 900 mg NMN for 60 days. Blood NAD concentration, blood biological age, homeostatic model assessment for insulin resistance, 6-minute walk test, and 36-item short-form survey (SF-36) were measured at baseline and after supplement...
February 29, 2024: Mechanisms of Ageing and Development
https://read.qxmd.com/read/38428685/nicotinamide-phosphoribosyltransferase-modulates-pd-l1-in-bladder-cancer-and-enhances-immunotherapeutic-sensitivity
#11
JOURNAL ARTICLE
Kuan-Chou Chen, Trayee Dhar, Chang-Rong Chen, Chang-Yu Chen, Chiung Chi Peng
Bladder cancer (BLCA) is one of the most prevalent malignancies worldwide with a high mortality rate and poor response to immunotherapy in patients expressing lower programmed death ligand 1 (PD-L1) levels. Nicotinamide phosphoribosyltransferase (NAMPT), a rate-limiting enzyme responsible for the biosynthesis of nicotinamide adenine dinucleotide (NAD+ ) from nicotinamide was reported to be overexpressed in various cancers; however, the role of NAMPT in BLCA is obscure. Immunohistochemistry of tissue microarrays, a real-time polymerase chain reaction, Western blotting, proliferation assay, NAD+ quantification, transwell-migration assay, and colony-formation assay were performed to measure NAMPT and PD-L1 expression levels in patients and the effect of NAMPT inhibition on T24 cells...
February 28, 2024: Biochimica et Biophysica Acta. Molecular Basis of Disease
https://read.qxmd.com/read/38422177/glycolysis-mediated-activation-of-v-atpase-by-nicotinamide-mononucleotide-ameliorates-lipid-induced-cardiomyopathy-by-repressing-the-cd36-tlr4-axis
#12
JOURNAL ARTICLE
Shujin Wang, Yinying Han, Ruimin Liu, Mengqian Hou, Dietbert Neumann, Jun Zhang, Fang Wang, Yumeng Li, Xueya Zhao, Francesco Schianchi, Chao Dai, Lizhong Liu, Miranda Nabben, Jan F C Glatz, Xin Wu, Xifeng Lu, Xi Li, Joost J F P Luiken
BACKGROUND: Chronic overconsumption of lipids followed by their excessive accumulation in the heart leads to cardiomyopathy. The cause of lipid-induced cardiomyopathy involves a pivotal role for the proton-pump vacuolar-type H+ -ATPase (v-ATPase), which acidifies endosomes, and for lipid-transporter CD36, which is stored in acidified endosomes. During lipid overexposure, an increased influx of lipids into cardiomyocytes is sensed by v-ATPase, which then disassembles, causing endosomal de-acidification and expulsion of stored CD36 from the endosomes toward the sarcolemma...
March 2024: Circulation Research
https://read.qxmd.com/read/38419707/long-term-follow-up-of-an-attenuated-presentation-of-naxe-related-disease-a-potentially-actionable-neurometabolic-disease-a-case-report
#13
Montaha Almudhry, Chitra Prasad, C Anthony Rupar, Keng Yeow Tay, Asuri N Prasad
BACKGROUND: Early-onset progressive encephalopathy with brain edema and/or leukoencephalopathy (PEBEL-1) is an autosomal recessive disorder whereby a fluctuating clinical course is exacerbated by febrile illnesses. Pathogenic NAD(P)HX epimerase ( NAXE ) gene mutations underpin this disorder. This mutation damages the metabolite repair system involved in regenerating crucial redox carriers. Longer survival has rarely been reported in this potentially actionable entity. OBJECTIVES: This case study aims to report a milder phenotype of a patient with NAXE gene mutation and his longitudinal follow-up of more than 20 years...
2024: Frontiers in Neurology
https://read.qxmd.com/read/38418756/functional-deterioration-of-vascular-mitochondrial-and-glycolytic-capacity-in-the-aortic-rings-of-aged-mice
#14
JOURNAL ARTICLE
Agnieszka Karaś, Anna Bar, Kanchana Pandian, Agnieszka Jasztal, Zuzanna Kuryłowicz, Barbara Kutryb-Zając, Elżbieta Buczek, Stefano Rocchetti, Tasnim Mohaissen, Agata Jędrzejewska, Amy C Harms, Patrycja Kaczara, Stefan Chłopicki
Vascular ageing is associated with increased arterial stiffness and cardiovascular mortality that might be linked to altered vascular energy metabolism. The aim of this study was to establish a Seahorse XFe96 Analyzer-based methodology for the reliable, functional assessment of mitochondrial respiration and glycolysis in single murine aortic rings and to validate this functional assay by characterising alterations in vascular energy metabolism in aged mice. Healthy young and old C57BL/6 mice were used for the analyses...
February 28, 2024: GeroScience
https://read.qxmd.com/read/38417968/a-study-protocol-to-investigate-if-acipimox-improves-muscle-function-and-sarcopenia-an-open-label-uncontrolled-before-and-after-experimental-medicine-feasibility-study-in-community-dwelling-older-adults
#15
JOURNAL ARTICLE
Claire McDonald, Craig Alderson, Matthew G Birkbeck, Laura Brown, Silvia Del Din, Grainne G Gorman, Kieren Hollingsworth, Clare Massarella, Rana Rehman, Lynn Rochester, Avan Ap Sayer, Huizhong Su, Helen Tuppen, Charlotte Warren, Miles D Witham
INTRODUCTION: Sarcopenia is the age-associated loss of muscle mass and strength. Nicotinamide adenine dinucleotide (NAD) plays a central role in both mitochondrial function and cellular ageing processes implicated in sarcopenia. NAD concentrations are low in older people with sarcopenia, and increasing skeletal muscle NAD concentrations may offer a novel therapy for this condition. Acipimox is a licensed lipid-lowering agent known to act as an NAD precursor. This open-label, uncontrolled, before-and-after proof-of-concept experimental medicine study will test whether daily supplementation with acipimox improves skeletal muscle NAD concentrations...
February 27, 2024: BMJ Open
https://read.qxmd.com/read/38399441/the-role-of-nad-and-nad-boosting-therapies-in-inflammatory-response-by-il-13
#16
REVIEW
Anton D Pugel, Alyssa M Schoenfeld, Sara Z Alsaifi, Jocelyn R Holmes, Brad E Morrison
The essential role of nicotinamide adenine dinucleotide+ (NAD+ ) in redox reactions during oxidative respiration is well known, yet the coenzyme and regulator functions of NAD+ in diverse and important processes are still being discovered. Maintaining NAD+ levels through diet is essential for health. In fact, the United States requires supplementation of the NAD+ precursor niacin into the food chain for these reasons. A large body of research also indicates that elevating NAD+ levels is beneficial for numerous conditions, including cancer, cardiovascular health, inflammatory response, and longevity...
February 8, 2024: Pharmaceuticals
https://read.qxmd.com/read/38398511/trimethylamine-n-oxide-improves-exercise-performance-by-reducing-oxidative-stress-through-activation-of-the-nrf2-signaling-pathway
#17
JOURNAL ARTICLE
Hong Zou, Yu Zhou, Lijing Gong, Caihua Huang, Xi Liu, Ruohan Lu, Jingjing Yu, Zhenxing Kong, Yimin Zhang, Donghai Lin
Trimethylamine N-oxide (TMAO) has attracted interest because of its association with cardiovascular disease and diabetes, and evidence for the beneficial effects of TMAO is accumulating. This study investigates the role of TMAO in improving exercise performance and elucidates the underlying molecular mechanisms. Using C2C12 cells, we established an oxidative stress model and administered TMAO treatment. Our results indicate that TMAO significantly protects myoblasts from oxidative stress-induced damage by increasing the expression of Nrf2, heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase (NQO1), and catalase (CAT)...
February 6, 2024: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/38397784/the-role-of-nicotinamide-mononucleotide-supplementation-in-psoriasis-treatment
#18
JOURNAL ARTICLE
Zhengyi Zhang, Baochen Cheng, Wenqian Du, Mengqi Zeng, Ke He, Tingyi Yin, Sen Shang, Tian Su, Dan Han, Xinyi Gan, Ziyang Wang, Meng Liu, Min Wang, Jiankang Liu, Yan Zheng
Psoriasis is one of several chronic inflammatory skin diseases with a high rate of recurrence, and its pathogenesis remains unclear. Nicotinamide mononucleotide (NMN), as an important precursor of nicotinamide adenine dinucleotide (NAD+), has been reported to be a promising agent in treating various diseases, its positive effects including those induced via its anti-inflammatory and antioxidant properties. For this reason, we have aimed to explore the possible role of NMN in the treatment of psoriasis. Psoriasis models were constructed with imiquimod (IMQ) stimulation for 5 days in vivo and with M5 treatment in keratinocyte cell lines in vitro...
February 1, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38397734/mulberry-leaf-polysaccharides-attenuate-oxidative-stress-injury-in-peripheral-blood-leukocytes-by-regulating-endoplasmic-reticulum-stress
#19
JOURNAL ARTICLE
Wenqiang Jiang, Yan Lin, Linjie Qian, Siyue Lu, Huaishun Shen, Xianping Ge, Linghong Miao
The present study assessed the protective effects and underlying mechanisms of mulberry leaf polysaccharides (MLPs) against hydrogen peroxide (H2 O2 )-induced oxidative stress injury in the peripheral blood leukocytes (PBLs) of Megalobrama amblycephala . Five treatment groups were established in vitro: the NC group (PBLs incubated in an RPMI-1640 complete medium for 4 h), the HP group (PBLs incubated in an RPMI-1640 complete medium for 3 h, and then stimulated with 100 μM of H2 O2 for 1 h), and the 50/100/200-MLP pre-treatment groups (PBLs were pre-treated with MLPs (50, 100, and 200 μg/mL) for 3 h, and then stimulated with 100 μM of H2 O2 for 1 h)...
January 23, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38392200/nicotinamide-riboside-regulates-chemotaxis-to-decrease-inflammation-and-ameliorate-functional-recovery-following-spinal-cord-injury-in-mice
#20
JOURNAL ARTICLE
Yan Li, Chunjia Zhang, Zihan Li, Fan Bai, Yingli Jing, Han Ke, Shuangyue Zhang, Yitong Yan, Yan Yu
Changes in intracellular nicotinamide adenine dinucleotide (NAD+ ) levels have been observed in various disease states. A decrease in NAD+ levels has been noted following spinal cord injury (SCI). Nicotinamide riboside (NR) serves as the precursor of NAD+ . Previous research has demonstrated the anti-inflammatory and apoptosis-reducing effects of NR supplements. However, it remains unclear whether NR exerts a similar role in mice after SCI. The objective of this study was to investigate the impact of NR on these changes in a mouse model of SCI...
February 1, 2024: Current Issues in Molecular Biology
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