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https://www.readbyqxmd.com/read/29352398/efficient-biosynthesis-of-l-phenylglycine-by-an-engineered-escherichia-coli-with-a-tunable-multi-enzyme-coordinate-expression-system
#1
Qiaoli Liu, Junping Zhou, Taowei Yang, Xian Zhang, Meijuan Xu, Zhiming Rao
Whole-cell catalysis with co-expression of two or more enzymes in a single host as a simple low-cost biosynthesis method has been widely studied and applied but hardly with regulation of multi-enzyme expression. Here we developed an efficient whole-cell catalyst for biosynthesis of L-phenylglycine (L-Phg) from benzoylformic acid through co-expression of leucine dehydrogenase from Bacillus cereus (BcLeuDH) and NAD+-dependent mutant formate dehydrogenase from Candida boidinii (CbFDHA10C) in Escherichia coli with tunable multi-enzyme-coordinate expression system...
January 19, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29351732/nutrient-depletion-and-tor-inhibition-induce-18s-and-25s-ribosomal-rnas-resistant-to-a-5-phosphate-dependent-exonuclease-in-candida-albicans-and-other-yeasts
#2
Jacob Fleischmann, Miguel A Rocha
BACKGROUND: Messenger RNA (mRNA) represents a small percentage of RNAs in a cell, with ribosomal RNA (rRNA) making up the bulk of it. To isolate mRNA from eukaryotes, typically poly-A selection is carried out. Recently, a 5´-phosphate-dependent, 5´→3´ processive exonuclease called Terminator has become available. It will digest only RNA that has a 5´-monophosphate end and therefore it is very useful to eliminate most of rRNAs in cell. RESULTS: We have found that in the pathogenic yeast Candida albicans, while 18S and 25S components isolated from yeast in robust growth phase are easily eliminated by Terminator, those isolated from cells in the nutritionally diminished stationary phase, become resistant to digestion by this enzyme...
January 19, 2018: BMC Molecular Biology
https://www.readbyqxmd.com/read/29350294/biocatalysis-of-aromatic-benzyl-propionate-ester-by-different-immobilized-lipases
#3
Amanda Gomes Almeida Sá, Alessandra Cristina de Meneses, Lindomar Alberto Lerin, Pedro Henrique Hermes de Araújo, Cláudia Sayer, Débora de Oliveira
Benzyl propionate is an aromatic ester that possesses a fruity odor and is usually found in nature in the composition of some fruits such as plums and melons. This work aimed for the benzyl propionate synthesis by esterification using a new immobilized enzyme preparation with low-cost material from Candida antarctica (NS 88011) and three commercial immobilized lipases (Novozym 435, Lipozyme TL-IM and Lipozyme RM-IM). Novozym 435 had the best performance even when the solvent tert-butanol was absent of the reaction medium...
January 19, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29348997/diagnosis-and-management-of-a-fatal-case-of-sepsis-caused-by-candida-parapsilosis-sensu-stricto-in-a-neonate-with-omphalocele
#4
Simone Santana, Tania Salci, Patricia Andriato, Patricia Bonfim-Mendonça, Silvana Caparroz-Assef, Melyssa Negri, Terezinha Svidzinski
We present a fatal case of persistent neonatal candidemia by Candida parapsilosis following omphalocele, without other anomalies. Despite an encouraging initial prognosis, after surgical correction and closure of the abdominal wall the case became difficult to treat, as in addition to the exposure of the patient to multiple risk factors for candidemia, antifungal therapy apparently was not adequate.
June 2018: Medical Mycology Case Reports
https://www.readbyqxmd.com/read/29348527/the-human-muscarinic-acetylcholine-receptor-antagonist-dicyclomine-targets-signal-transduction-genes-and-inhibits-the-virulence-factors-in-the-human-pathogen-candida-albicans
#5
Awad Ali, Ashwini Jadhav, Priyanka Jangid, Rajendra Patil, Amruta Shelar, Sankunny Mohan Karuppayil
Dicyclomine is a human muscarinic acetylcholine receptor antagonist used for the treatment of abdominal cramps. We are reporting here that dicyclomine can inhibit the in vitro growth and virulence factors of the human pathogen Candida albicans very effectively. Dicyclomine inhibited adhesion, early biofilm, mature biofilm, and planktonic growth. Yeast to hyphal form transition of C. albicans in various inducer media such as serum, proline, glucose, and N-acetylglucosamine was inhibited. Dicyclomine also could kill C...
January 18, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29346584/in-vitro-interactions-between-iap-antagonist-at406-and-azoles-against-planktonic-cells-and-biofilms-of-pathogenic-fungi-candida-albicans-and-exophiala-dermatitidis
#6
Yi Sun, Lujuan Gao, Chengyan He, Ming Li, Tongxiang Zeng
In vitro interactions of AT406, a novel IAP antagonist, and azoles including itraconazole, voriconazole, and fluconazole against planktonic cells and biofilms of Candida albicans and Exophiala dermatitidis were assessed via broth microdilution checkerboard technique. AT406 alone exhibited limited antifungal activity. However, synergistic effect between AT406 and fluconazole was observed against both planktonic cells and biofilms of C. albicans, including one fluconazole-resistant strain. Moreover, synergism was also demonstrated between AT406 and itraconazole against both planktonic cells and biofilms of E...
January 13, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29346565/apigenin-induces-cell-shrinkage-in-candida-albicans-by-membrane-perturbation
#7
Heejeong Lee, Eun-Rhan Woo, Dong Gun Lee
Apigenin, a natural flavone, has been well characterized for its their anticarcinogenic property; however, its bioactivity against pathogenic fungi has not been investigated in detail. In the present study, we examined the antifungal activity and mode of action of apigenin. Apigenin inhibited the growth of fungal pathogens, which induced superficial infection and reduced biofilm mass. Three-dimensional flow cytometric analysis demonstrated that apigenin induced morphological changes, especially cell shrinkage, in Candida albicans...
January 16, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29346561/floricolin-c-elicits-intracellular-ros-accumulation-and-disrupts-mitochondria-to-exert-fungicidal-action
#8
Ming Zhang, Wenqiang Chang, Hongzhuo Shi, Ying Li, Sha Zheng, Wei Li, Hongxiang Lou
Candida albicans, one most prevalent fungal pathogen, causes severe mucosal and invasive infections in predisposed individuals. The rise of fungal infection and drug-resistance demands the development of novel antifungal agents. In this study, we observed that floricolin C (FC), a p-terphenyl pigment from an endolichenic fungus, killed C. albicans cells at planktonic state or within biofilms through reactive oxygen species (ROS) accumulation. Further test revealed that FC could directly damage the mitochondria to cause ROS accumulation...
January 15, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29346493/antimicrobial-studies-using-the-therapeutic-tissue-cross-linking-agent-sodium-hydroxymethylglycinate-implication-for-treating-infectious-keratitis
#9
Patrick B Rapuano, Alexandra H Scanameo, Daeryl E Amponin, Sefy A Paulose, Mariya Zyablitskaya, Anna Takaoka, Leejee H Suh, Takayuki Nagasaki, Stephen L Trokel, David C Paik
Purpose: Our recent studies raise the possibility of using sodium hydroxymethylglycinate (SMG), for pharmacologic therapeutic tissue cross-linking (TXL) of the cornea. The present study was performed to evaluate the antimicrobial effects of SMG for potential use in treating infectious keratitis. Methods: In initial (group 1) experiments, methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa (PA) were treated with SMG (10-40 mM) for 10 to 120 minutes...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29346398/dual-function-of-edta-with-silver-nanoparticles-for-root-canal-treatment-a-novel-modification
#10
Juan M Martinez-Andrade, Miguel Avalos-Borja, Alfredo R Vilchis-Nestor, Luis O Sanchez-Vargas, Ernestina Castro-Longoria
The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively...
2018: PloS One
https://www.readbyqxmd.com/read/29346071/identification-of-potential-inhibitors-against-nuclear-dam1-complex-subunit-ask1-of-candida-albicans-using-virtual-screening-and-md-simulations
#11
Himanshu Tripathi, Feroz Khan
Identification of hit compounds against specific target form the starting point for a drug discovery program. A consistent decline of new chemical entities (NCEs) in recent years prompted a challenge to explore newer approaches to discover potential hit compounds that in turn can be converted into leads, and ultimately drug with desired therapeutic efficacy. The vast amount of omics and activity data available in public databases offers an opportunity to identify novel targets and their potential inhibitors...
January 1, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29345117/candida-auris-a-systematic-review-and-meta-analysis-of-current-updates-on-an-emerging-multidrug-resistant-pathogen
#12
John Osei Sekyere
From 2009, Candida auris has emerged as a multidrug-resistant ascomycete yeast pathogen with the capacity for easy transmission between patients and hospitals, as well as persistence on environmental surfaces. Its association with high mortalities, breakthrough and persistent candidaemia, inconsistencies in susceptibility testing results, misidentification by available commercial identification systems and treatment failure, complicates its management and detection. Within the last nine years, C. auris has been increasingly reported from far-Eastern Asia, the Middle East, Africa, Europe, South and North America with substantial fatalities and misidentification...
January 18, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29344749/diagnosis-of-sleep-apnea-in-patients-with-stable-chronic-heart-failure-using-a-portable-sleep-test-diagnostic-device
#13
Inês Araújo, Filipa Marques, Sandra André, Manuel Araújo, Sara Marques, Rita Ferreira, Patrícia Moniz, Margarida Proença, Pedro Borrego, Cândida Fonseca
PURPOSE: ApneaLink is a portable device for the screening of sleep apnea, a prevalent and underdiagnosed comorbidity in heart failure patients. A prospective cross-sectional study in patients with chronic heart failure was carried out to assess the sensitivity and specificity of apnea-hypopnea index (AHI) measurements using ApneaLink against the standard polysomnography test. METHODS: Adult patients with a prior hospitalization in an acute heart failure hospital unit were recruited for the study...
January 17, 2018: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/29343748/sac7-and-rho1-regulate-the-white-to-opaque-switching-in-candida-albicans
#14
Siwy Ling Yang, Guisheng Zeng, Fong Yee Chan, Yan-Ming Wang, Dongliang Yang, Yue Wang
Candida albicans cells homozygous at the mating-type locus stochastically undergo the white-to-opaque switching to become mating-competent. This switching is regulated by a core circuit of transcription factors organized through interlocking feedback loops around the master regulator Wor1. Although a range of distinct environmental cues is known to induce the switching, the pathways linking the external stimuli to the central control mechanism remains largely unknown. By screening a C. albicans haploid gene-deletion library, we found that SAC7 encoding a GTPase-activating protein of Rho1 is required for the white-to-opaque switching...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29342119/novel-hybrid-formulations-based-on-thiourea-derivatives-and-core-shell-fe%C3%A2-o%C3%A2-c18-nanostructures-for-the-development-of-antifungal-strategies
#15
Carmen Limban, Alexandru Vasile Missir, Miron Teodor Caproiu, Alexandru Mihai Grumezescu, Mariana Carmen Chifiriuc, Coralia Bleotu, Luminita Marutescu, Marius Toma Papacocea, Diana Camelia Nuta
The continuously increasing global impact of fungal infections is requiring the rapid development of novel antifungal agents. Due to their multiple pharmacological activities, thiourea derivatives represent privileged candidates for shaping new drugs. We report here the preparation, physico-chemical characterization and bioevaluation of hybrid nanosystems based on new 2-((4-chlorophenoxy)methyl)-N-(substituted phenylcarbamo-thioyl)benzamides and Fe₃O₄@C18 core@shell nanoparticles. The new benzamides were prepared by an efficient method, then their structure was confirmed by spectral studies and elemental analysis and they were further loaded on Fe₃O₄@C18 nanostructures...
January 17, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29342100/modulation-of-the-fungal-host-interaction-by-the-intra-species-diversity-of-c-albicans
#16
REVIEW
Christina Braunsdorf, Salomé LeibundGut-Landmann
The incidence of human infections caused by the opportunistic fungal pathogen Candida albicans is on the rise due to increasing numbers of immunosuppressed patients. The importance of the immune system in preventing overgrowth of the colonizing fungus and thereby limiting infection is well recognized and host protective mechanisms widely investigated. Only recently, it was recognized that the natural diversity in the fungal species could also influence the outcome of the interaction between the fungus and the host...
January 17, 2018: Pathogens
https://www.readbyqxmd.com/read/29341887/molecular-basis-of-substrate-polyspecificity-of-the-candida-albicans-mdr1p-multidrug-h-antiporter
#17
Archana Kumari Redhu, Atanu Banerjee, Abdul Haseeb Shah, Alexis Moreno, Manpreet Kaur Rawal, Remya Nair, Pierre Falson, Rajendra Prasad
The molecular basis of polyspecificity of Mdr1p, a major drug/H+ antiporter of Candida albicans, is not elucidated. We have probed the nature of the drug-binding pocket by performing systematic mutagenesis of the 12 transmembrane segments. Replacement of the 252 amino acid residues with alanine or glycine yielded 2/3 neutral mutations while 1/3 led to the complete or selective lost of resistance to drugs or substrates transported by the pump. Using the GlpT-based 3D-model of Mdr1p, we roughly categorized these critical residues depending on their type and localization 1°/ main structural impact ("S" group), 2°/ exposure to the lipid interface ("L" group), 3°/ buried but not facing the main central pocket, inferred as critical for the overall H+/drug antiport mechanism ("M" group) and finally 4°/ buried and facing the main central pocket ("B" group)...
January 13, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29340981/nanolipid-gel-of-an-antimycotic-drug-for-treating-vulvovaginal-candidiasis-development-and-evaluation
#18
Dnyanesh Takalkar, Namita Desai
This paper focuses on the development and evaluation of mucoadhesive vaginal gel of fluconazole using nanolipid carriers to enhance tissue deposition in treating vulvovaginal candidiasis. Treatment of vulvovaginal candidiasis includes antimycotic agents prescribed for 1 to 7 days or longer, in relapse either orally or topically. The delivery of fluconazole as nanolipid carriers in vaginal gel can be proposed as suitable alternative to the existing conventional formulations to improve the patient acceptability, compliance and localized drug action...
January 16, 2018: AAPS PharmSciTech
https://www.readbyqxmd.com/read/29340656/antagonistic-effect-of-saccharomyces-cerevisiae-ktp-and-issatchenkia-occidentalis-apc-on-hyphal-development-and-adhesion-of-candida-albicans
#19
K Lohith, K A Anu-Appaiah
The morphological transition from yeast to a hyphal form, as well as the adhesion capability to the gastrointestinal tract, are implicated virulent determinant in Candida albicans and could be potential targets for prevention of the opportunistic pathogen. Based on this rationale, two yeast strains Saccharomyces cerevisiae KTP and Issatchenkia occidentalis ApC along with reference strain Saccharomyces boulardii NCDC 363 were screened for the probiotic potential. Characters like pH, temperature, bile, simulated gastrointestinal juice tolerance tests, and Caco-2 cell line adhesion assay were determined in the present study...
January 11, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29340465/variability-in-the-clinical-distributions-of-candida-species-and-the-emergence-of-azole-resistant-non-candida-albicans-species-in-public-hospitals-in-the-midwest-region-of-brazil
#20
Karine Mattos, Luana Carbonera Rodrigues, Kelly Mari Pires de Oliveira, Pedro Fernando Diniz, Luiza Inahê Marques, Adriana Almeida Araujo, Marilene Rodrigues Chang
INTRODUCTION: Incidence and antifungal susceptibility of Candida spp. from two teaching public hospitals are described. METHODS: The minimum inhibitory concentrations of fluconazole, voriconazole, itraconazole, and amphotericin B were determined using Clinical Laboratory Standard Institute broth microdilution and genomic differentiation using PCR. RESULTS: Of 221 Candida isolates, 50.2% were obtained from intensive care unit patients; 71.5% were recovered from urine and 9...
November 2017: Revista da Sociedade Brasileira de Medicina Tropical
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