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https://www.readbyqxmd.com/read/29902206/modification-of-n6-methyladenosine-rna-methylation-on-heat-shock-protein-expression
#1
Jiayao Yu, Yi Li, Tian Wang, Xiang Zhong
This study was conducted to investigate effect of N6-methyladenosine (m6A) RNA methylation on Heat shock proteins (HSPs) and dissect the profile of HSP RNA methylation. The results showed that m6A methyltransferases METTL3 mRNA was decreased in responses to heat shock stress in HepG2 cells, but m6A-specific binding protein YTHDF2 mRNA was upregulated in a manner similar to HSP70 induction. Immunofluorescence staining showed that the majority of YTHDF2 was present in the cytosol, however, nearly all YTHDF2 translocated from the cytosol into the nucleus after heat shock...
2018: PloS One
https://www.readbyqxmd.com/read/29892502/beef-tenderness-and-intramuscular-fat-proteomic-biomarkers-muscle-type-effect
#2
Brigitte Picard, Mohammed Gagaoua, Marwa Al-Jammas, Leanne De Koning, Albéric Valais, Muriel Bonnet
Tenderness and intramuscular fat content are key attributes for beef sensory qualities. Recently some proteomic analysis revealed several proteins which are considered as good biomarkers of these quality traits. This study focuses on the analysis of 20 of these proteins representative of several biological functions: muscle structure and ultrastructure, muscle energetic metabolism, cellular stress and apoptosis. The relative abundance of the proteins was measured by Reverse Phase Protein Array (RPPA) in five muscles known to have different tenderness and intramuscular lipid contents: Longissimus thoracis (LT), Semimembranosus (SM), Rectus abdominis (RA), Triceps brachii (TB) and Semitendinosus (ST)...
2018: PeerJ
https://www.readbyqxmd.com/read/29883750/antitumor-activity-of-nanoliposomes-encapsulating-the-novobiocin-analogue-6brcaq-in-a-triple-negative-breast-cancer-model-in-mice
#3
Félix Sauvage, Elias Fattal, Walhan Al-Shaer, Stéphanie Denis, Emilie Brotin, Christophe Denoyelle, Cécile Blanc-Fournier, Balthazar Toussaint, Samir Messaoudi, Mouad Alami, Gillian Barratt, Juliette Vergnaud-Gauduchon
In this study, we investigated the anticancer efficacy of pegylated liposomes containing 6BrCaQ, an hsp90 inhibitor derived from novobiocin. 6BrCaQ has been previously identified as the most potent compound in a series of quinoleic novobiocin analogs but is poorly water-soluble. We investigated, for the first time, the anti-proliferative effects of this drug in vivo in an orthotopic breast cancer model (MDA-MB-231 luc) using pegylated liposomes to allow its administration. Hsp90, hsp70 and hsp27 protein and mRNA expressions were not strongly affected after treatment meaning it did not induce a heat shock response often associated with resistance and poor prognosis...
June 5, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29875161/a-potential-strategy-for-reducing-cysts-in-autosomal-dominant-polycystic-kidney-disease-with-a-cftr-corrector
#4
Murali Yanda, Qiangni Liu, Liudmila Cebotaru
Autosomal dominant polycystic kidney disease (ADPKD) is associated with progressive enlargement of cysts, leading to a decline in function and renal failure that cannot be prevented by current treatments. Mutations in pkd1 and pkd2, encoding the polycystin 1 and 2 proteins, induce growth-related pathways, including heat shock proteins, as occurs in some cancers raising the prospect that pharmacological interventions that target these pathways might alleviate or prevent ADPKD. Here, we demonstrate a role for VX-809, a corrector of cystic fibrosis transmembrane conductance regulator (CFTR) conventionally used to manage cystic fibrosis, in reducing renal cyst growth...
June 6, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29870887/matrix-metalloproteinase-2-a-key-regulator-in-coagulation-proteases-mediated-human-breast-cancer-progression-through-autocrine-signaling
#5
Kaushik Das, Ramesh Prasad, Shabbir Ahmed Ansari, Abhishek Roy, Ashis Mukherjee, Prosenjit Sen
AIMS: Cell invasion is attributed to the synthesis and secretion of proteolytically active matrix-metalloproteinases (MMPs) by tumor cells to degrade extracellular matrix (ECM) and promote metastasis. The role of protease-activated receptor 2 (PAR2) in human breast cancer migration/invasion via MMP-2 up-regulation remains ill-defined; hence we investigated whether TF-FVIIa/trypsin-mediated PAR2 activation induces MMP-2 expression in human breast cancer. MAIN METHODS: MMP-2 expression and the signaling mechanisms were analyzed by western blotting and RT-PCR...
June 2, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29864522/chemokine-cc-motif-ligand-2-participates-in-platelet-function-and-arterial-thrombosis-by-regulating-pkc%C3%AE-p38mapk-hsp27-pathway
#6
Dan Liu, Yu Cao, Xiaolin Zhang, Chengfei Peng, Xiaoxiang Tian, Chenghui Yan, Yanxia Liu, Meili Liu, Yaling Han
BACKGROUND: Studies indicate that chemokine CC-motif ligand 2 (CCL2) is involved in inflammation and atherosclerosis. However, the roles and mechanisms of CCL2 on platelet function and arterial thrombosis are unknown. METHODS: The expressions of CCL2 or CCR2 in the plasma, platelets and coronary thrombus of ST-elevated myocardial infarction (STEMI) patients were examined by ELISA, Western blot, immunohistochemistry and immunofluorescence. The roles of CCL2 on platelet aggregation, activation and secretion were examined by light transmission aggregometry, flow cytometry and ELISA...
June 1, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29845259/shrna%C3%A2-induced-knockdown-of-the-spert-gene-inhibits-proliferation-and-promotes-apoptosis-of-human-colorectal-cancer-rko-cells
#7
Long-Zhi Zheng, Si-Zeng Chen
Colorectal cancer is the third most common type of cancer and the fourth leading cause of cancer‑related deaths worldwide. Although several genes have been identified to contribute to the pathogenesis of colorectal cancer, there are still many genes with unidentified functions in colorectal cancer. This study aimed to investigate the effect of shRNA‑induced knockdown of the SPERT gene on the proliferation and apoptosis of human colorectal cancer RKO cells. SPERT was screened based on the TCGA dataset, and SPERT expression, cell growth, proliferation and apoptosis were detected in shSPERT‑ and shCtrl‑transfected RKO cells...
May 21, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29844172/mesenchymal-mapkapk2-hsp27-drives-intestinal-carcinogenesis
#8
Ana Henriques, Vasiliki Koliaraki, George Kollias
Mesenchymal cells in the microenvironment of cancer exert important functions in tumorigenesis; however, little is known of intrinsic pathways that mediate these effects. MAPK signals, such as from MAPKAPK2 (MK2) are known to modulate tumorigenesis, yet their cell-specific role has not been determined. Here, we studied the cell-specific role of MK2 in intestinal carcinogenesis using complete and conditional ablation of MK2. We show that both genetic and chemical inhibition of MK2 led to decreased epithelial cell proliferation, associated with reduced tumor growth and invasive potential in the Apc min/+ mouse model...
May 29, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29808718/targeting-of-pp2c%C3%AE-by-a-small-molecule-c23-inhibits-high-glucose-induced-breast-cancer-progression-in-vivo
#9
Ke Wu, Xiaoting Yu, Zhimin Huang, Donghui Zhu, Xianghua Yi, Ying-Li Wu, Qiongyu Hao, Kevin T Kemp, Yahya Elshimali, Roshni Iyer, Kytai Truong Nguyen, Shilong Zheng, Guanglin Chen, Qiao-Hong Chen, Guangdi Wang, Jaydutt V Vadgama, Yong Wu
AIMS: Epidemiologic evidence indicates that diabetes may increase risk of breast cancer (BC) and mortality in patients with cancer. The pathophysiological relationships between diabetes and cancer are not fully understood and personalized treatments for diabetes-associated BC are urgently needed. RESULTS: We observed that high glucose (HG), via activation of nuclear phosphatase PP2Cδ, suppresses p53 function and consequently promotes BC cell proliferation, migration and invasion...
May 29, 2018: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29803474/synergistic-action-of-cathepsin-b-l-d-and-calpain-in-disassembly-and-degradation-of-myofibrillar-protein-of-grass-carp
#10
Lihong Ge, Yanshun Xu, Wenshui Xia, Qixing Jiang
The objective of this study was to investigate the differential role of cathepsin B, L, D and calpain in degradation and disassembly of myofilament. Myofibrillar protein of grass carp (Ctenopharyngodon idella) was incubated with proteases monotonously, simultaneously or sequentially. Subsequently, protein degradation were detected using SDS-PAGE and myofilament disassembly induced by changes of non-covalent interactions were measured through SDS-PAGE using l-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide (EDC) as a zero length cross-linker...
July 2018: Food Research International
https://www.readbyqxmd.com/read/29795667/phosphorylated-recombinant-hsp27-protects-the-brain-and-attenuates-blood-brain-barrier-disruption-following-stroke-in-mice-receiving-intravenous-tissue-plasminogen-activator
#11
Yoshiaki Shimada, Hideki Shimura, Ryota Tanaka, Kazuo Yamashiro, Masato Koike, Yasuo Uchiyama, Takao Urabe, Nobutaka Hattori
Loss of integrity of the blood-brain barrier (BBB) in ischemic stroke victims initiates a devastating cascade of events causing brain damage. Maintaining the BBB is important to preserve brain function in ischemic stroke. Unfortunately, recombinant tissue plasminogen activator (tPA), the only effective fibrinolytic treatment at the acute stage of ischemic stroke, also injures the BBB and increases the risk of brain edema and secondary hemorrhagic transformation. Thus, it is important to identify compounds that maintain BBB integrity in the face of ischemic injury in patients with stroke...
2018: PloS One
https://www.readbyqxmd.com/read/29792338/molecular-mechanism-for-the-influence-of-gender-dimorphism-on-alcoholic-liver-injury-in-mice
#12
S-Q Li, P Wang, D-M Wang, H-J Lu, R-F Li, L-X Duan, S Zhu, S-L Wang, Y-Y Zhang, Y-L Wang
It is known that women develop alcoholic liver injury more rapidly and have a lower alcohol toxic threshold than men. However, the detailed molecular mechanisms remain unclear. The precise mechanism responsible for the sex difference needs to be determined. Female and male mice were given ethanol by intragastric infusion every day for 4 weeks. The pathological changes were detected by hematoxylin-eosin, Sirius red, oil red O, periodic acid-Schiff, and Hochest33258 staining in the liver of female and male mice...
January 1, 2018: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29774100/differential-proteomic-profile-of-leukemic-cd34-progenitor-cells-from-chronic-myeloid-leukemia-patients
#13
Maria Rosaria Ricciardi, Valentina Salvestrini, Roberto Licchetta, Simone Mirabilii, Mattia Forcato, Gabriele Gugliotta, Simona Salati, Fausto Castagnetti, Gianantonio Rosti, Massimo Breccia, Giuliana Alimena, Rossella Manfredini, Silvio Bicciato, Roberto Massimo Lemoli, Agostino Tafuri
Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of quiescent cells which are to some extent resistant to tyrosine kinase inhibitors (TKIs). BCR-ABL oncogene activates multiple cross-talking signal transduction pathways (STP), such as RAS/MEK/ERK, PI3K/Akt, Wnt and STAT5, contributing to abnormal proliferation of clonal cells. From this perspective, the aim of this study was to analyze the expression and activation profile of STP involved in the mechanisms of cell proliferation/quiescence and survival of the progenitor CD34+ cells from chronic phase (CP) CML...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29766408/association-between-circulating-levels-of-heat-shock-protein-27-and-aggressive-periodontitis
#14
Frank Kaiser, Nikos Donos, Brian Henderson, Rajesh Alagarswamy, George Pelekos, David Boniface, Luigi Nibali
Heat-shock protein (Hsp) 27 is a major intracellular molecular chaperone and controller of intracellular responses to inflammatory signals. In the extracellular space, recombinant Hsp27 has been described to exert anti-inflammatory activities. The aim of this study was to assess the association between circulating levels of Hsp27 and different types of periodontitis. Pro- and anti-inflammatory cytokines and the stress proteins Hsp27 and Hsp60 with proposed anti- and pro-inflammatory properties, respectively, were measured by two-site ELISA in the serum of patients with aggressive periodontitis (AgP, n = 30), chronic periodontitis (CP, n = 29) and periodontally healthy controls (H, n = 28)...
May 15, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29765151/apatorsen-plus-docetaxel-versus-docetaxel-alone-in-platinum-resistant-metastatic-urothelial-carcinoma-borealis-2
#15
Jonathan E Rosenberg, Noah M Hahn, Meredith M Regan, Lillian Werner, Ajjai Alva, Saby George, Joel Picus, Robert Alter, Arjun Balar, Jean Hoffman-Censits, Petros Grivas, Richard Lauer, Elizabeth A Guancial, Christopher Hoimes, Guru Sonpavde, Constantine Albany, Mark N Stein, Tim Breen, Cindy Jacobs, Kirsten Anderson, Joaquim Bellmunt, Aly-Khan A Lalani, Sumanta Pal, Toni K Choueiri
BACKGROUND: A randomised study to assess the addition of apatorsen, an antisense oligonucleotide that inhibits Hsp27 expression, to docetaxel in patients with metastatic urothelial carcinoma (mUC) relapsed after prior platinum-based chemotherapy. METHODS: Multicentre, phase II study with 1:1 randomisation to apatorsen (three loading doses at 600 mg intravenous followed by weekly doses) plus docetaxel (75 mg/m2 intravenous every 21 days) (A/D) or docetaxel alone...
May 16, 2018: British Journal of Cancer
https://www.readbyqxmd.com/read/29729335/sec6-enhances-cell-migration-and-suppresses-apoptosis-by-elevating-the-phosphorylation-of-p38-mapk-mk2-and-hsp27
#16
Toshiaki Tanaka, Mitsuyoshi Iino, Kaoru Goto
The signaling axis of p38 mitogen-activated protein kinase (p38 MAPK) and MAPK-activated protein kinase 2 (MK2) is the dominant pathway that leads to heat shock protein 27 (HSP27) phosphorylation. After activation of MK2 by p38 MAPK, HSP27 is phosphorylated and depolymerized by MK2, thereby increasing the cell migration and directly interfering with the apoptotic signaling cascades. Sec6 is one of the components of the exocyst complex that is an evolutionarily conserved 8-protein complex. Even though several studies have demonstrated that Sec6 is involved in various cellular physiological functions, the relationship between Sec6 and HSP27 or p38 MAPK during cell migration and apoptosis remains unclear...
May 2, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29717105/long-noncoding-rna-dancr-promotes-colorectal-cancer-proliferation-and-metastasis-via-mir-577-sponging
#17
Yong Wang, Zhi Lu, Ningnin Wang, Jianzhou Feng, Junjie Zhang, Lan Luan, Wei Zhao, Xiandong Zeng
Long non-coding RNAs (lncRNAs) play key roles in various malignant tumors, including colorectal cancer (CRC). Long non-coding RNA differentiation antagonizing non-protein coding RNA (DANCR) is overexpressed in CRC patients, but whether it affects CRC proliferation and metastasis via regulation of heat shock protein 27 (HSP27) remains unclear. In the present study, we found that DANCR was highly expressed and correlated with proliferation and metastasis in CRC. In addition, we demonstrated that DANCR and HSP27 were both targets of microRNA-577 (miR-577) and shared the same binding site...
May 1, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29699584/androgen-receptor-and-heat-shock-protein-27-co-regulate-the-malignant-potential-of-molecular-apocrine-breast-cancer
#18
Xiaozhen Liu, Changyun Feng, Junjun Liu, Lu Cao, Guomin Xiang, Fang Liu, Shuling Wang, Jiao Jiao, Yun Niu
BACKGROUND: The most striking feature of molecular apocrine breast cancer (MABC) is the expression of androgen receptor (AR). We report here the mechanism of the AR in regulating the behavior of MABC. METHODS: The MABC cell line, MDA-MB-453, and the nonMABC cell line, MCF7, were used in this study. The effect of dihydrotestosterone (DHT) and heat shock protein 27 (HSP27) on cell proliferation was quantified using the cell counter kit-8 (CCK8) and clonogenic assays in vitro and by a xenograft tumor model in vivo...
April 27, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29695571/heat-shock-protein-27-expression-in-eus-fna-samples-can-predict-gemcitabine-sensitivity-in-pancreatic-cancer
#19
Michitaka Kawano, Seiji Kaino, Shogo Amano, Shuhei Shinoda, Shigeyuki Suenaga, Manabu Sen-Yo, Isao Sakaida
BACKGROUND/AIM: Gemcitabine (GEM) sensitivity can help select the appropriate treatment for pancreatic cancer. We examined the association between HSP27 expression and GEM sensitivity. MATERIALS AND METHODS: A total of 19 patients with unresectable pancreatic cancer who underwent endoscopic ultrasonography-guided fine needle aspiration (EUS-FNA) were enrolled and treated with GEM alone. We measured the expression of heat shock protein 27 (HSP27) and phosphorylated HSP27(p-HSP27) in EUS-FNA samples and evaluated the effects of GEM treatment...
May 2018: In Vivo
https://www.readbyqxmd.com/read/29682518/association-of-high-cardiovascular-fitness-and-the-rate-of-adaptation-to-heat-stress
#20
Małgorzata Żychowska, Alicja Nowak-Zaleska, Grzegorz Chruściński, Ryszard Zaleski, Jan Mieszkowski, Bartłomiej Niespodziński, Roman Tymański, Andrzej Kochanowicz
This study aimed to compare changes in genes expression associated with inflammation and apoptosis in response to heat stress caused by sauna between people with varying cardiorespiratory fitness levels. We hypothesis that high cardiorespiratory level caused higher positive changes after four weeks of sauna bathing. Blood samples were taken at rest before and after the first and last sauna sessions and 48 hours after the last sauna session and used to assay HSP70 (HSPA1A) , HSP27 (HSPB1) , interleukin 6 (IL6) , and interleukin 10 (IL10) genes expression in blood with quantitative real-time qRT-PCR...
2018: BioMed Research International
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