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amine oxidase

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https://www.readbyqxmd.com/read/29198609/synthesis-and-evaluation-of-biaryl-derivatives-for-structural-characterization-of-selective-monoamine-oxidase-b-inhibitors-toward-parkinson-s-disease-therapy
#1
Seul Ki Yeon, Ji Won Choi, Jong-Hyun Park, Ye Rim Lee, Hyeon Jeong Kim, Su Jeong Shin, Bo Ko Jang, Siwon Kim, Yong-Sun Bahn, Gyoonhee Han, Yong Sup Lee, Ae Nim Pae, Ki Duk Park
Benzyloxyphenyl moiety is a common structure of highly potent, selective and reversible inhibitors of monoamine oxidase B (MAO-B), safinamide and sembragiline. We synthesized 4-(benzyloxy)phenyl and biphenyl-4-yl derivatives including halogen substituents on the terminal aryl unit. In addition, we modified the carbon linker between amine group and the biaryl linked unit. Among synthesized compounds, 12c exhibited the most potent and selective MAO-B inhibitory effect (hMAO-B IC50: 8.9 nM; >10,000-fold selectivity over MAO-A) as a competitive inhibitor...
November 24, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29193544/characterisation-of-a-bacterial-galactokinase-with-high-activity-and-broad-substrate-tolerance-for-chemoenzymatic-synthesis-of-6-aminogalactose-1-phosphate-and-analogues
#2
Kun Huang, Fabio Parmeggiani, Edward Pallister, Chuen-Jiuan Huang, Fang-Fang Liu, Qian Li, William R Birmingham, Peter Both, Baptiste Thomas, Li Liu, Josef Voglmeir, Sabine Flitsch
Glycosyl phosphates are important intermediates in many metabolic pathways and are substrates for diverse carbohydrate active enzymes. There is a need to develop libraries of structurally similar analogues that can be used as selective chemical probes in glycomics. Here we explore chemoenzymatic cascades for the fast generation of glycosyl phosphate libraries without protecting group strategies. The key enzyme is a new bacterial galactokinase (LgGalK) cloned from Leminorella grimontii which was produced in E...
November 29, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29187599/oligomannosidic-glycans-at-asn110-are-essential-for-secretion-of-human-diamine-oxidase
#3
Elisabeth Gludovacz, Daniel Maresch, Leonor Lopes de Carvalho, Verena Puxbaum, Laurenz J Baier, Leander Sützl, Gabriela Guédez, Clemens Grünwald-Gruber, Barbara Ulm, Sophie Pils, Robin Ristl, Friedrich Altmann, Bernd Jilma, Tiina A Salminen, Nicole Borth, Thomas Boehm
N-glycosylation plays a fundamental role in many biological processes. Human diamine oxidase (hDAO), required for hista-mine catabolism, has multiple N-glycosylation sites, but their roles, for example in DAO secretion, are unclear. We recently reported that the N-glycosylation sites Asn168, Asn538, and Asn745 in recombinant hDAO (rhDAO) carry complex-type glycans, where-as Asn110 carries only mammalian-atypical oligomannosidic glycans. Here, we show that Asn110 in native hDAO from amniotic fluid and Caco-2 cells, DAO from porcine kidneys, and rhDAO produced in two different HEK293 cell lines is also consistently occu-pied by oligomannosidic glycans...
November 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29184001/pace4-proteolytically-processes-loxl2-with-little-impact-on-its-catalytic-activity
#4
Kazushi Okada, Hee-Jung Moon, Joel Finney, Frédéric Couture, Robert Day, Minae Mure
Lysyl oxidase-like 2 (LOXL2) belongs to the LOX family of amine oxidases, which play a critical role in extracellular matrix remodeling by crosslinking collagen and elastin. Overexpression of LOX and/or LOXL2 drives fibrosis in several hepatic and vascular diseases, and tumor progression in some aggressive cancers. Because LOX and LOXL2 have a conserved Cterminal catalytic domain, LOXL2 is generally presumed to have the same biological activity as LOX. However, their N-termini are remarkably different and hint at potentially discrete functions and regulation...
November 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29175057/simvastatin-blocks-soluble-ssao-vap-1-release-in-experimental-models-of-cerebral-ischemia-possible-benefits-for-stroke-induced-inflammation-control
#5
Ping Sun, Mar Hernandez-Guillamón, Mireia Campos-Martorell, Alba Simats, Joan Montaner, Mercedes Unzeta, Montse Solé
Beyond cholesterol reduction, statins mediate their beneficial effects on stroke patients through pleiotropic actions. They have shown anti-inflammatory properties by a number of different mechanisms, including the inhibition of NF-κB transcriptional activity and the consequent increase and release of adhesion molecules. We have studied simvastatin's effects on the vascular enzyme semicarbazide-sensitive amine oxidase/vascular adhesion protein 1 (SSAO/VAP-1), which is involved in stroke-mediated brain injury...
November 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29158336/severe-fertility-effects-of-sheepish%C3%A2-sperm-caused-by-failure-to-enter-female-sperm-storage-organs-in-drosophila-melanogaster
#6
Masatoshi Tomaru, Takashi Ohsako, Masahide Watanabe, Naoto Juni, Hiroshi Matsubayashi, Hiromi Sato, Ayako Takahashi, Masa-Toshi Yamamoto
In Drosophila, mature sperm are transferred from males to females during copulation, stored in the sperm storage organs of females, and then utilized for fertilization. Here, we report a gene named sheepish (shps) of D. melanogaster that is essential for sperm storage in females. shps mutant males, although producing morphologically normal and motile sperm that are effectively transferred to females, produce very few offspring. Direct counts of sperm indicated that the primary defect was correlated to failure of shps sperm to migrate into the female sperm storage organs...
November 20, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29155974/transcript-profiling-of-a-bitter-variety-of-narrow-leafed-lupin-to-discover-alkaloid-biosynthetic-genes
#7
Ting Yang, Istvan Nagy, Davide Mancinotti, Sophie Lisa Otterbach, Trine Bundgaard Andersen, Mohammed Saddik Motawia, Torben Asp, Fernando Geu-Flores
Lupins (Lupinus spp.) are nitrogen-fixing legumes that accumulate toxic alkaloids in their protein-rich beans. These anti-nutritional compounds belong to the family of quinolizidine alkaloids (QAs), which are of interest to the pharmaceutical and chemical industries. To unleash the potential of lupins as protein crops and as sources of QAs, a thorough understanding of the QA pathway is needed. However, only the first enzyme in the pathway, lysine decarboxylase (LDC), is known. Here, we report the transcriptome of a high-QA variety of narrow-leafed lupin (L...
November 16, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29148316/recognition-dynamics-of-trinuclear-copper-cluster-and-associated-histidine-residues-through-conserved-or-semi-conserved-water-molecules-in-human-ceruloplasmin-the-involvement-of-aspartic-and-glutamic-acid-gates
#8
Bishnu Prasad Mukhopadhyay
Human Ceruloplasmin belongs to the family of multi-copper oxidases and it is involved in different physiological processes, copper ion transport, iron metabolism, iron homeostasis, and biogenic amine metabolism. MD-simulation studies have indicated the higher hydrophilic susceptibility of the trinuclear copper cluster in native CP compared to its oxygen bound form. The copper (T2/T3) atom Cu3047 of the cluster, which is close to T1 copper center Cu3052 (~13 Å) has a higher affinity for water molecules compared to other copper centers...
November 17, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29144021/quantitative-extracellular-matrix-proteomics-suggests-cell-wall-reprogramming-in-host-specific-immunity-during-vascular-wilt-caused-by-fusarium-oxysporum-in-chickpea
#9
Eman Elagamey, Kanika Narula, Arunima Sinha, Sudip Ghosh, Magdi A E Abdellatef, Niranjan Chakraborty, Subhra Chakraborty
Extracellular matrix (ECM) is the unique organelle that perceives stress signals and reprograms molecular events of host cell during patho-stress. However, our understanding of how ECM dictates plant immunity is largely unknown. Vascular wilt caused by the soil borne filamentous fungus Fusarium oxysporum is a major impediment for global crop productivity. To elucidate the role of ECM proteins and molecular mechanism associated with cell wall mediated immunity, the temporal changes of ECM proteome was studied in vascular wilt resistant chickpea cultivar upon F...
November 16, 2017: Proteomics
https://www.readbyqxmd.com/read/29135319/crosslinking-enzyme-lysyl-oxidase-modulates-scleral-remodeling-in-form-deprivation-myopia
#10
Ying Yuan, Min Li, Qingzhong Chen, Rao Me, Yunjie Yu, Qing Gu, Guangsen Shi, Bilian Ke
PURPOSE: Scleral remodeling causes the excessive ocular elongation that underlies myopia. Lysyl oxidase (LOX), a copper-containing amine oxidase, can catalyze collagen and elastin crosslinking. The purpose of this study was to investigate the role of LOX in scleral remodeling in form-deprivation myopia (FDM). METHODS: Seventy-five guinea pigs were randomly divided into five groups as follows: a normal control group, an FDM group, an FDM plus β-aminopropionitrile (BAPN) group, an FDM plus TGF-β1 (TGF-β1) group, and an FDM plus vehicle group...
November 14, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29127553/identification-of-histidine-303-as-the-catalytic-base-of-lysyl-oxidase-via-site-directed-mutagenesis
#11
Rachel N Oldfield, Kathryn A Johnston, Jeanette Limones, Caitlin Ghilarducci, Karlo M Lopez
Lysyl oxidase (LOX) is a copper-dependent amine oxidase enzyme that catalyzes the formation of crosslinkages of collagen and elastin in connective tissues by oxidative deamination of lysine. Using site-directed mutagenesis, Histidine 303 has been shown to be a key residue that acts as the necessary catalytic base for this enzyme to function properly. Histidine 303 was mutated to isoleucine to remove catalytic activity and to aspartate and glutamate, respectively, in order to provide alternate residues that could act as a general base that could maintain catalytic activity...
November 10, 2017: Protein Journal
https://www.readbyqxmd.com/read/29116116/urinary-sulphatoxymelatonin-as-a-biomarker-of-serotonin-status-in-biogenic-amine-deficient-patients
#12
Marta Batllori, Marta Molero-Luis, Luisa Arrabal, Javier de Las Heras, Joaquín-Alejandro Fernandez-Ramos, Luis González Gutiérrez-Solana, Salvador Ibáñez-Micó, Rosario Domingo, Jaume Campistol, Aida Ormazabal, Frederic Sedel, Thomas Opladen, Basiliki Zouvelou, Roser Pons, Angels Garcia-Cazorla, Eduardo Lopez-Laso, Rafael Artuch
Melatonin is synthesized from serotonin and it is excreted as sulphatoxymelatonin in urine. We aim to evaluate urinary sulphatoxymelatonin as a biomarker of brain serotonin status in a cohort of patients with mutations in genes related to serotonin biosynthesis. We analized urinary sulphatoxymelatonin from 65 healthy subjects and from 28 patients with genetic defects. A total of 18 patients were studied: 14 with autosomal dominant and recessive guanosine triphosphate cyclohydrolase-I deficiency; 3 with sepiapterin reductase deficiency; and 1 with aromatic L-amino acid decarboxylase deficiency...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29100099/synthesis-of-chiral-amines-using-redox-biocatalysis
#13
REVIEW
Gideon Grogan
Chiral amines feature in a large number of small molecule pharmaceuticals, and thus methods for their asymmetric synthesis are of considerable interest. Biocatalytic approaches have come to the fore in recent years as these offer advantages of superior atom economy, mild reaction conditions and excellent stereoselectivity. Advances in redox cofactor process technology have meant that oxidoreductase enzymes in particular now have growing potential as industrial catalysts for amine formation. In this review we survey recent developments in the discovery and application of oxidoreductase enzymes for amine production, including Monoamine Oxidases (MAOs), engineered and natural Amine Dehydrogenases (AmDHs), Imine Reductases (IREDs) and Reductive Aminases (RedAms), in addition to their application in enzyme cascades...
October 31, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/29080184/real-time-in-vivo-monitoring-of-reactive-oxygen-species-in-guard-cells
#14
Ky Young Park, Kalliopi A Roubelakis-Angelakis
The intra-/intercellular homeostasis of reactive oxygen species (ROS), and especially of superoxides (O2(.-)) and hydrogen peroxide (O2(.-)) participate in signalling cascades which dictate developmental processes and reactions to biotic/abiotic stresses. Polyamine oxidases terminally oxidize/back convert polyamines generating H2O2. Recently, an NADPH-oxidase/Polyamine oxidase feedback loop was identified to control oxidative burst under salinity. Thus, the real-time localization/monitoring of ROS in specific cells, such as the guard cells, can be of great interest...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29080170/analysis-of-the-intracellular-localization-of-transiently-expressed-and-fluorescently-labeled-copper-containing-amine-oxidases-diamine-oxidase-and-n-methylputrescine-oxidase-in-tobacco-using-an-agrobacterium-infiltration-protocol
#15
Tsubasa Shoji
The intracellular localization of enzymes provides key information for understanding complex metabolic pathways. Based on enzyme localization data, the involvement of multiple organelles and the movement of metabolites between cellular compartments have been suggested for a number of pathways. Transient expression of fluorescently tagged proteins in the leaves of Nicotiana benthamiana through Agrobacterium infiltration is a simple and versatile way to examine the intracellular localization of proteins of interest...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29080163/determination-of-copper-amine-oxidase-activity-in-plant-tissues
#16
Riccardo Angelini, Alessandra Cona, Paraskevi Tavladoraki
Copper amine oxidases (CuAOs) involved in polyamine catabolism are emerging as physiologically relevant enzymes for their involvement in plant growth, differentiation and defence responses to biotic and abiotic stress. In this chapter, we describe two spectrophotometric and one polarographic method for determining CuAO activity in plant tissues. Some aspects related to cell wall association of apoplastic CuAOs and possible interference of plant metabolites with the enzymatic activity assays are also considered...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29080157/development-of-amine-oxidase-based-biosensors-for-spermine-and-spermidine-analysis
#17
Cristina Tortolini, Gabriele Favero, Franco Mazzei
In this work a detailed description of the development of amine oxidase-based electrochemical biosensors for the selective determination of the biogenic amines is presented. The enzymes required for this operation are Polyamine Oxidase (PAO) and Spermine Oxidase (SMO) which are physically entrapped in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ), a photo-cross-linkable gel, onto screen printed electrode (SPE) surface. The developed biosensors are deeply characterized in the analysis of biogenic amines by using flow injection amperometric (FIA) technique...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29065711/vascular-adhesion-protein-1-a-cell-surface-amine-oxidase-in-translation
#18
Sirpa Jalkanen, Marko Salmi
SIGNIFICANCE: Vascular adhesion protein-1 (VAP-1) is an ectoenzyme, which oxidates primary amines in a reaction producing also hydrogen peroxide. VAP-1 on the blood vessel endothelium regulates leukocyte extravasation from the blood into tissues under physiological and pathological conditions. Recent advances: Inhibition of VAP-1 by neutralizing antibodies and by several novel small molecule enzyme inhibitors interferes with leukocyte trafficking and alleviates inflammation in many experimental models...
October 25, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29054367/monoamine-oxidase-b-inhibitor-protects-degenerating-spinal-neurons-enhances-nerve-regeneration-and-functional-recovery-in-sciatic-nerve-crush-injury-model
#19
Al-Shimali M Hussain, Waleed M Renno, Hanaa L Sadek, Noura M Kayali, Aseel Al-Salem, Muddanna S Rao, Khalid M Khan
Monoamine oxidase-B (MAOB), a flavin adenine dinucleotide (FAD), is an enzyme which catalyzes the oxidation of amines. MAOB is proposed to play a major role in the pathogenesis of neurodegeneration through the production of reactive oxygen species (ROS) and neurotoxins. The present study was designed to outline the effects of the MAOB inhibitor (MAOB-I) on neuroprotection of spinal neurons, regeneration of sciatic nerve fibers, and recovery of sensory-motor functions in the sciatic nerve crush injury model...
October 17, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29054071/inactivation-of-bovine-plasma-amine-oxidase-by-1-1-1-trihalo-3-aminopropanes
#20
Jisook Kim, Irene N Lee
In this paper, we report the inactivation of copper containing bovine plasma amine oxidase (BPAO) by a series of saturated alkylamines containing halogen atoms at γ-position, which are 1,1,1-trihalo-3-aminopropane, 1,1,1-trifluoro-2-hydroxy-3-aminopropane, 1,1,1-trichloro-2-hydroxy-3-aminopropane, and 1,1,1-trichloro-2-(2-phenethyloxy)-3-aminopropane. The trihalo-2-hydroxypropylamine analogs exhibited a time-dependent inactivation behavior of BPAO, with 1,1,1-trifluoro-2-hydroxy-3-aminopropane as the most efficient inactivator...
October 16, 2017: Bioorganic Chemistry
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