Rui Yang, Amanda S Meyer, Ilia A Droujinine, Namrata D Udeshi, Yanhui Hu, Jinjin Guo, Jill A McMahon, Dominique K Carey, Charles Xu, Qiao Fang, Jihui Sha, Shishang Qin, David Rocco, James Wohlschlegel, Alice Y Ting, Steven A Carr, Norbert Perrimon, Andrew P McMahon
Organ functions are highly specialized and interdependent. Secreted factors regulate organ development and mediate homeostasis through serum trafficking and inter-organ communication. Enzyme-catalysed proximity labelling enables the identification of proteins within a specific cellular compartment. Here, we report a BirA*G3 mouse strain that enables CRE-dependent promiscuous biotinylation of proteins trafficking through the endoplasmic reticulum. When broadly activated throughout the mouse, widespread labelling of proteins was observed within the secretory pathway...
August 2022: Open Biology