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https://www.readbyqxmd.com/read/28334300/flight-capacity-of-the-walnut-twig-beetle-coleoptera-scolytidae-on-a-laboratory-flight-mill
#1
Aubree M Kees, Andrea R Hefty, Robert C Venette, Steven J Seybold, Brian H Aukema
The walnut twig beetle, Pityophthorus juglandis Blackman, and associated fungus Geosmithia morbida Kolařík, Freeland, Utley, & Tisserat constitute the insect-fungal complex that causes thousand cankers disease in walnut, Juglans spp., and wingnut, Pterocarya spp. Thousand cankers disease is responsible for the decline of Juglans species throughout the western United States and more recently, the eastern United States and northern Italy. We examined the flight capacity of P. juglandis over 24-h trials on a flight mill in the laboratory...
March 16, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/28315215/detection-of-food-allergens-by-phage-displayed-produced-antibodies
#2
Raquel Madrid, Silvia de la Cruz, Aina García, Rosario Martín, Isabel González, Teresa García
Phage display is a powerful tool to produce recombinant antibodies against a given antigen without animal immunization. This technology employs libraries of recombinant bacteriophages that display billions of different functional antibody fragments on their surface. They are selected by panning in vitro against the target antigen in search for specific binders. In this chapter, we describe the selection of single chain variable fragment (scFv) antibodies to be used for detection of allergenic proteins from nuts in food products...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28315214/detection-of-food-allergens-by-taqman-real-time-pcr-methodology
#3
Aina García, Raquel Madrid, Teresa García, Rosario Martín, Isabel González
Real-time PCR (polymerase chain reaction) has shown to be a very effective technology for the detection of food allergens. The protocol described herein consists on a real-time PCR assay targeting the plant ITS (Internal Transcribed Spacer) region, using species-specific primers and hydrolysis probes (Taqman) dual labeled with a reporter fluorophore at the 5' end (6-carboxyfluorescein, FAM) and a quencher fluorophore at the 3' end (Blackberry, BBQ). The species-specific real-time PCR systems (primers/probe) described in this work allowed the detection of different nuts (peanut, hazelnut, pistachio, almond, cashew, macadamia, walnut and pecan), common allergens present in commercial food products, with a detection limit of 0...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28303393/effective-nut-dispersal-by-magpies-pica-pica-l-in-a-mediterranean-agroecosystem
#4
Jorge Castro, Mercedes Molina-Morales, Alexandro B Leverkus, Loreto Martínez-Baroja, Lorenzo Pérez-Camacho, Pedro Villar-Salvador, Salvador Rebollo, José M Rey-Benayas
Scatter-hoarding animals such as corvids play a crucial role in the dispersal of nut-producing tree species. This interaction is well known for some corvids, but remains elusive for other species such as the magpie (Pica pica), an abundant corvid in agroecosystems and open landscapes of the Palearctic region. In addition, the establishment of the individual dispersed seeds-a prerequisite for determining seed-dispersal effectiveness-has never before been documented for the interaction between corvids and nut-producing trees...
March 16, 2017: Oecologia
https://www.readbyqxmd.com/read/28289857/-neuronal-like-differentiation-of-murine-mesenchymal-stem-cell-line-stimulation-by-juglans-regia-l-oil
#5
Varsha Singh, Mingma Sherpa
Mesenchymal stem cells have been extensively used for cell-based therapies especially in neuronal diseases. Studies still continue to delineate mechanisms involved in differentiating mesenchymal stem cells into neuronal cells under experimental conditions as they have low mortality rate and hence, the number of cells available for experiments is much more limited. Culturing and differentiating of neuronal cell is more challenging as they do not undergo cell division thus, bringing them to differentiate proves to be a difficult task...
March 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28287049/impact-of-processing-conditions-on-the-phytoprostanes-profile-of-three-types-of-nut-kernels
#6
Ana María Carrasco-Del Amor, Encarna Aguayo, Jacinta Collado-González, Alexandre Guy, Jean-Marie Galano, Thierry Durand, Ángel Gil-Izquierdo
The goal of this work was the identification and quantification of phytoprostanes (PhytoPs) in three types of nuts: "Walnut", "Macadamia", and "Pecan". This study represents a first approach to the relationship between the quantitative and qualitative PhytoP profiles in the "Macadamia" and "Pecan" nuts subjected to fried salt or fried honey processing. The kernels were found to contain 9-F1t-PhytoP, 9-epi-9-F1t-PhytoP, 16-B1-PhytoP, ent-16-B1-PhytoP, 9-L1-PhytoP, and ent-9-L1-PhytoP. "Macadamia" fried salt nuts were the only ones that produced 9-epi-9-D1-PhytoP and 9-D1-PhytoP...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28286541/an-evaluation-of-inexpensive-methods-for-root-image-acquisition-when-using-rhizotrons
#7
Awaz Mohamed, Yogan Monnier, Zhun Mao, Guillaume Lobet, Jean-Luc Maeght, Merlin Ramel, Alexia Stokes
BACKGROUND: Belowground processes play an essential role in ecosystem nutrient cycling and the global carbon budget cycle. Quantifying fine root growth is crucial to the understanding of ecosystem structure and function and in predicting how ecosystems respond to climate variability. A better understanding of root system growth is necessary, but choosing the best method of observation is complex, especially in the natural soil environment. Here, we compare five methods of root image acquisition using inexpensive technology that is currently available on the market: flatbed scanner, handheld scanner, manual tracing, a smartphone application scanner and a time-lapse camera...
2017: Plant Methods
https://www.readbyqxmd.com/read/28266839/walnut-like-porous-core-shell-tio2-with-hybridized-phases-enabling-fast-and-stable-lithium-storage
#8
Yi Cai, Hongen Wang, Xu Zhao, Fei Huang, Chao Wang, Zhao Deng, Yu Li, Guozhong Cao, Bao-Lian Su
TiO2 is a promising and safe anode material for lithium ion batteries (LIBs). However, its practical application has been plagued by its poor rate capability and cycling properties. Herein, we successfully demonstrate a novel structured TiO2 anode with excellent rate capability and ultra-long cycle life. The TiO2 material reported here shows a walnut-like porous core/shell structure with hybridized anatase/amorphous phases. The effective synergy of the unique walnut-like porous core/shell structure, the phase hybridization with nanoscale coherent heterointerfaces, as well as the presence of minor carbon species endows the TiO2 material with superior lithium storage properties in terms of high capacity (~177 mA h g-1 at 1 C, 1 C=170 mA g-1), good rate capability (62 mA h g-1 at 100 C), and excellent cycling stability (~83 mA h g-1 was retained over 10000 cycles at 10 C with a capacity decay of 0...
March 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28265357/antioxidant-potential-of-juglans-nigra-black-walnut-husks-extracted-using-supercritical-carbon-dioxide-with-an-ethanol-modifier
#9
Jonathan Wenzel, Cheryl Storer Samaniego, Lihua Wang, Laron Burrows, Evan Tucker, Nathan Dwarshuis, Michelle Ammerman, Ali Zand
The black walnut, Junglas nigra, is indigenous to eastern North America, and abscission of its fruit occurs around October. The fruit consists of a husk, a hard shell, and kernel. The husk is commonly discarded in processing, though it contains phenolic compounds that exhibit antioxidant and antimicrobial properties. For this study, black walnut husks were extracted using supercritical carbon dioxide with an ethanol modifier. The effects of temperature, ethanol concentration, and drying of walnut husks prior to extraction upon antioxidant potential were evaluated using a factorial design of experiments...
March 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28261916/probable-walnut-induced-anaphylactic-reaction-in-a-dog
#10
Ana Rostaher, Nina Maria Fischer, Claudia Kümmerle-Fraune, Nicolas Couturier, Sandrine Jacquenet, Claude Favrot
BACKGROUND: Anaphylaxis due to nuts is frequent in humans; to the best of the authors' knowledge, it has not been reported previously in dogs. CASE REPORT: A 5-year-old female, intact, Vizsla dog was presented with acute diarrhoea, vomiting, respiratory distress and erythematous wheals. The dog had eaten walnuts, which she had been fed in small amounts for years, hours before the onset of clinical signs. A diagnosis of generalized anaphylaxis was made. Skin testing and Western blotting revealed positive results with walnuts and hazelnuts...
April 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28257470/rethinking-the-history-of-common-walnut-juglans-regia-l-in-europe-its-origins-and-human-interactions
#11
Paola Pollegioni, Keith Woeste, Francesca Chiocchini, Stefano Del Lungo, Marco Ciolfi, Irene Olimpieri, Virginia Tortolano, Jo Clark, Gabriel E Hemery, Sergio Mapelli, Maria Emilia Malvolti
Common walnut (Juglans regia L) is an economically important species cultivated worldwide for its high-quality wood and nuts. It is generally accepted that after the last glaciation J. regia survived and grew in almost completely isolated stands in Asia, and that ancient humans dispersed walnuts across Asia and into new habitats via trade and cultural expansion. The history of walnut in Europe is a matter of debate, however. In this study, we estimated the genetic diversity and structure of 91 Eurasian walnut populations using 14 neutral microsatellites...
2017: PloS One
https://www.readbyqxmd.com/read/28250581/genome-wide-comparative-analysis-of-leafy-promoter-sequence-in-angiosperms
#12
Zhichao Sun, Zheng Li, Jianqin Huang, Bingsong Zheng, Liangsheng Zhang, Zhengjia Wang
Regulation of the flowering mechanism is influenced by many environmental factors. Dissecting the regulatory processes upstream of the LFY (LEAFY) gene will help us to understand the molecular mechanisms of floral induction. In total, 53 LFY sequences were identified in 37 species. Among the 53 selected LFY promoters and after eliminating the short sequences, 47 LFY promoters were analyzed. Comparative genome studies for LFY promoters among plants showed that TATA-box existed in all herbaceous plants. The 1345-bp promoter sequence upstream to hickory LFY gene was cloned and analyzed, together with functional studies...
January 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28243724/in-vitro-gene-expression-and-mrna-translocation-from-transformed-walnut-juglans-regia-rootstocks-expressing-dsred-fluorescent-protein-to-wild-type-scions
#13
Xiaochen Liu, Sriema L Walawage, Charles A Leslie, Abhaya M Dandekar, David M Tricoli, Hengkang Hu, Youjun Huang, Jiaqi Zhang, Chuanmei Xv, Jianqin Huang, Qixiang Zhang
An in vitro grafting method was developed for examining gene translocation from rootstock to scion in walnut. Results showed the DsRED gene itself was not translocated but expressed mRNA was. Grafting is widely used in plants, especially in fruit and nut crops. Selected rootstocks can control scion growth and physiological traits, including shortening of the juvenile phase and controlling tree size. Rootstocks also can provide improved soil adaptation and pathogen resistance. Development of genetically modified (GM) fruit crops has progressed recently, but commercial cultivation is still limited due to the time required for evaluation and issues with deregulation...
February 27, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28242460/assessing-the-biodegradability-of-microparticles-disposed-down-the-drain
#14
Kathleen McDonough, Nina Itrich, Kenneth Casteel, Jennifer Menzies, Tom Williams, Kady Krivos, Jason Price
Microparticles made from naturally occurring materials or biodegradable plastics such as poly(3-hydroxy butyrate)-co-(3-hydroxy valerate), PHBV, are being evaluated as alternatives to microplastics in personal care product applications but limited data is available on their ultimate biodegradability (mineralization) in down the drain environmental compartments. An OECD 301B Ready Biodegradation Test was used to quantify ultimate biodegradability of microparticles made of PHBV foam, jojoba wax, beeswax, rice bran wax, stearyl stearate, blueberry seeds and walnut shells...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/28239673/effectiveness-of-a-walnut-enriched-diet-on-murine-sperm-involvement-of-reduced-peroxidative-damage
#15
Lauren S Coffua, Patricia A Martin-DeLeon
A walnut supplement for a Western-style diet in men was shown to improve sperm motility, vitality, and morphology. To gain further insights into factors underlying this improvement, we administered a parallel walnut-enriched diet to mice [including those with a defect in sperm motility due to deletion of Plasma Membrane Ca(2+)-ATPase 4 (Pmca4(-/-) )] to determine if there is a similar improvement that is accompanied by reduced sperm membrane peroxidative damage. Although sperm vitality and acrosome reaction rate were unaffected, the diet led to a significant improvement in motility (P < 0...
February 2017: Heliyon
https://www.readbyqxmd.com/read/28238332/randomized-clinical-trial-of-the-effectiveness-of-complementary-therapies-for-recurrent-aphthous-stomatitis
#16
Alberto Rodríguez-Archilla, Tarik Raissouni
BACKGROUND AND OBJECTIVE: Despite the high prevalence of recurrent aphthous stomatitis (RAS), its etiology is not yet completely clear and there is no completely remedial treatment available at present. The objective of this study was to evaluate the clinical efficacy and safety of 4 treatments (silver nitrate, propolis, rhubarb and walnut) for RAS. PATIENTS AND METHOD: A randomized clinical trial was conducted with 125 patients with minor aphthae, including 25 patients per group: cauterization with silver nitrate, propolis, rhubarb extract, walnut extract and placebo...
February 18, 2017: Medicina Clínica
https://www.readbyqxmd.com/read/28214700/uncertainty-estimation-by-bayesian-approach-in-thermochemical-conversion-of-walnut-hull-and-lignite-coal-blends
#17
Musa Buyukada
The main purpose of the present study was to incorporate the uncertainties in the thermal behavior of walnut hull (WH), lignite coal, and their various blends using Bayesian approach. First of all, thermal behavior of related materials were investigated under different temperatures, blend ratios, and heating rates. Results of ultimate and proximate analyses showed the main steps of oxidation mechanism of (co-)combustion process. Thermal degradation started with the (hemi-)cellulosic compounds and finished with lignin...
February 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28209093/two-new-cytotoxic-glycosides-isolated-from-the-green-walnut-husks-of-juglans-mandshurica-maxim
#18
Yuan-Yuan Zhou, Quan-Yu Liu, Bing-You Yang, Yan-Qiu Jiang, Yu-Xin Liu, Ying Wang, Shuang Guo, Haixue Kuang
Two new glycosides including an alcohol glycoside and a phenolic glycoside: hexyl-1-O-α-d-arabinofuranosyl-(1 → 6)-β-d-glucopyranoside (1), 4-hydroxypropiophenone-4-O-β-d-glucopyranosyl(1 → 6)-β-d-glucopyranoside(2), along with six known naphthalenyl glucosides (3-8) were isolated from green walnut husks of Juglans mandshurica, and their structures were elucidated on the basis of spectroscopic studies. All compounds were evaluated for their inhibitory effects on tumour cells (BGC-823, HepG-2, MCF-7)...
June 2017: Natural Product Research
https://www.readbyqxmd.com/read/28207311/prevalence-of-salmonella-in-cashews-hazelnuts-macadamia-nuts-pecans-pine-nuts-and-walnuts-in-the-united-states
#19
Guodong Zhang, Lijun Hu, David Melka, Hua Wang, Anna Laasri, Eric W Brown, Errol Strain, Marc Allard, Vincent K Bunning, Steven M Musser, Rhoma Johnson, Sofia M Santillana Farakos, Virginia N Scott, Régis Pouillot, Jane M Van Doren, Thomas S Hammack
Nuts have been identified as a vector for salmonellosis. The objective of this project was to estimate the prevalence and contamination level of Salmonella in raw tree nuts (cashews, pecans, hazelnuts, macadamia nuts, pine nuts, and walnuts) at retail markets in the United States. A total of 3,656 samples of six types of tree nuts were collected from different types of retail stores and markets nationwide between October 2014 and October 2015. These samples were analyzed using a modified version of the Salmonella culture method from the U...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28191338/accelerated-burn-wound-closure-in-mice-with-a-new-formula-based-on-traditional-medicine
#20
Mehrnaz Mehrabani, Seyyed Mohsen Seyyedkazemi, Mohammad Hadi Nematollahi, Elham Jafari, Mitra Mehrabani, Mohammad Mehdipour, Zahra Sheikhshoaee, Ali Mandegary
BACKGROUND: A combination of the oils of sesame, hemp, wild pistachio, and walnut has been used for treatment of skin disorders, including wound burns, in some parts of Kerman, Iran. Evaluation of this remedy in the form of a pharmaceutical formulation in animal models can pave the way for its future application in wound burn healing in humans. OBJECTIVES: This experimental study investigated the healing potential of a new formula (NF) based on folk medicine from Iran for the treatment of third degree burns in mice...
November 2016: Iranian Red Crescent Medical Journal
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