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https://www.readbyqxmd.com/read/29932078/an-improved-microsatellite-panel-to-assess-genetic-variability-of-the-italian-smooth-newt-lissotriton-vulgaris-meridionalis
#1
Vincenzo Buono, Giorgia Galliani, Emiliano Mancini, Francesca Davoli, Chiara Mengoni, Nadia Mucci, Leonardo Vignoli
Habitat loss and fragmentation are threatening amphibians by increasing population isolation. However, artificial waterbodies created for livestockmay contrast this phenomenon by providing surrogate habitats for amphibians. Here, we performed a genetic study on an amphibian species, Lissotriton vulgaris meridionalis , in a rural area in central Italy where natural wetlands are disappearing and drinking troughs for cattle watering are widespread. Specifically, we tested a panel of microsatellite markers to identify a suitable tool for addressing conservation genetic issues of this species that is undergoing severe local decline...
June 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29924214/effect-of-red-propolis-on-hamster-cheek-pouch-angiogenesis-in-a-new-sponge-implant-model
#2
Nayanna de Oliveira Ramos Melo, Camila de Carvalho Juanes, Mayara Freire de Alencar Alves, Emiliano Tiago Melo Silva, Francisco Vagnaldo Fechine Jamacaru, Telma Leda Gomes de Lemos, Conceição Aparecida Dornelas
PURPOSE: To evaluate the effects of red propolis on cheek pouch angiogenesis in a hamster new model sponge implant. METHODS: Forty eight animals divided into eight groups. (Groups I-IV), the animals were treated for 15 days before and 10 days after sponge implantation. (Groups V-VIII), the animals were treated for 10 days after sponge implantation (GI and GV: red propolis 100 mg/kg, GII and GVI: celecoxib 20 mg/kg, GIII and GVII: 1% gum arabic 5 mL/kg, GIV and GVIII: distilled water 5 mL/kg)...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29922173/the-effects-of-acute-and-chronic-sprint-interval-training-on-cytokine-responses-are-independent-of-prior-caffeine-intake
#3
Guilherme A Ferreira, Leandro C Felippe, Rômulo Bertuzzi, David J Bishop, Emiliano Barreto, Fernando R De-Oliveira, Adriano E Lima-Silva
We examined the effect of acute and chronic sprint interval training (SIT), with or without prior caffeine intake, on levels of exercise-induced inflammatory plasma cytokines [interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-α]. Twenty physically-active men ingested either a placebo ( n = 10) or caffeine ( n = 10) 1 h before each SIT session(13-s × 30-s sprint/15 s of rest) during six training sessions (2 weeks). The early (before, immediately after, and 45 min after the exercise) and late (24 and 48 h after the exercise) cytokine and creatine kinase (CK) responses were analyzed for the first and last training sessions...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29921721/air-in-the-portal-space-where-does-it-come-from
#4
Ramón Elizondo-Romo, Jorge Adrián Nuñez-Zuno, Iván Josué Jiménez-González, Emiliano Montiel-Nuñez, José Víctor Jiménez, Diego Luis Carrillo-Pérez
No abstract text is available yet for this article.
June 19, 2018: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/29921520/comparison-of-marker-models-for-the-analysis-of-the-volume-variation-and-thoracoabdominal-motion-pattern-in-untrained-and-trained-participants
#5
Carlo Massaroni, Amanda Piaia Silvatti, Irisz Karolina Levai, John Dickinson, Samantha Winter, Emiliano Schena, Sergio Silvestri
Respiratory assessment and the biomechanical analysis of chest and abdomen motion during breathing can be carried out using motion capture systems. An advantage of this methodology is that it allows analysis of compartmental breathing volumes, thoraco-abdominal patterns, percentage contribution of each compartment and the coordination between compartments. In the literature, mainly, two marker models are reported, a full marker model of 89 markers placed on the trunk and a reduced marker model with 32 markers...
June 15, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29920189/oncogenic-trk-fusions-are-amenable-to-inhibition-in-hematologic-malignancies
#6
Justin Taylor, Dean Pavlick, Akihide Yoshimi, Christina Marcelus, Stephen S Chung, Jaclyn F Hechtman, Ryma Benayed, Emiliano Cocco, Benjamin H Durham, Lillian Bitner, Daichi Inoue, Young Rock Chung, Kerry Mullaney, Justin M Watts, Eli L Diamond, Lee A Albacker, Tariq I Mughal, Kevin Ebata, Brian B Tuch, Nora Ku, Maurizio Scaltriti, Mikhail Roshal, Maria Arcila, Siraj Ali, David M Hyman, Jae H Park, Omar Abdel-Wahab
Rearrangements involving the neurotrophic receptor kinase genes (NTRK1, NTRK2, and NTRK3; hereafter referred to as TRK) produce oncogenic fusions in a wide variety of cancers in adults and children. Although TRK fusions occur in <1% of all solid tumors, inhibition of TRK results in profound therapeutic responses resulting in breakthrough FDA-approval of the TRK inhibitor larotrectinib for adult and pediatric solid tumor patients regardless of histology. In contrast to solid tumors, the frequency of TRK fusions and clinical effects of targeting TRK in hematologic malignancies is unknown...
June 19, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29915686/tackling-critical-parameters-in-metazoan-meta-barcoding-experiments-a-preliminary-study-based-on-coxi-dna-barcode
#7
Bachir Balech, Anna Sandionigi, Caterina Manzari, Emiliano Trucchi, Apollonia Tullo, Flavio Licciulli, Giorgio Grillo, Elisabetta Sbisà, Stefano De Felici, Cecilia Saccone, Anna Maria D'Erchia, Donatella Cesaroni, Maurizio Casiraghi, Saverio Vicario
Nowadays DNA meta-barcoding is a powerful instrument capable of quickly discovering the biodiversity of an environmental sample by integrating the DNA barcoding approach with High Throughput Sequencing technologies. It mainly consists of the parallel reading of informative genomic fragment/s able to discriminate living entities. Although this approach has been widely studied, it still needs optimization in some necessary steps requested in its advanced accomplishment. A fundamental element concerns the standardization of bioinformatic analyses pipelines...
2018: PeerJ
https://www.readbyqxmd.com/read/29915285/guided-morphogenesis-through-optogenetic-activation-of-rho-signalling-during-early-drosophila-embryogenesis
#8
Emiliano Izquierdo, Theresa Quinkler, Stefano De Renzis
During organismal development, cells undergo complex changes in shape whose causal relationship to individual morphogenetic processes remains unclear. The modular nature of such processes suggests that it should be possible to isolate individual modules, determine the minimum set of requirements sufficient to drive tissue remodeling, and re-construct morphogenesis. Here we use optogenetics to reconstitute epithelial folding in embryonic Drosophila tissues that otherwise would not undergo invagination. We show that precise spatial and temporal activation of Rho signaling is sufficient to trigger apical constriction and tissue folding...
June 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29909839/lipoxygenases-and-lipoxygenase-products-in-marine-diatoms
#9
Giuliana d'Ippolito, Genoveffa Nuzzo, Angela Sardo, Emiliano Manzo, Carmela Gallo, Angelo Fontana
Marine diatoms negatively affect reproduction and later larval development of dominant zooplankton grazers such as copepods, thereby lowering the recruitment of the next generations of these small crustaceans that are a major food source for larval fish species. The phenomenon has been explained in terms of chemical defense due to grazer-induced synthesis of oxylipins, lipoxygenase-derived oxygenated fatty acid derivatives. Since this first report, studies about diatom oxylipins have multiplied and broadened toward other aspects concerning bloom dynamics, cell growth, and cell differentiation...
2018: Methods in Enzymology
https://www.readbyqxmd.com/read/29909823/sterol-sulfates-and-sulfotransferases-in-marine-diatoms
#10
Carmela Gallo, Genoveffa Nuzzo, Giuliana d'Ippolito, Emiliano Manzo, Angela Sardo, Angelo Fontana
Sterol sulfates are widely occurring molecules in marine organisms. Their importance has been so far underestimated although many of these compounds are crucial mediators of physiological and ecological functions in other organisms. Biosynthesis of sterol sulfates is controlled by cytosolic sulfotransferases (SULTs), a varied family of enzymes that catalyze the transfer of a sulfo residue (-SO3 H) from the universal donor 3'-phosphoadenosine-5'-phosphosulfate to the hydroxyl function at C-3 of the steroid skeleton...
2018: Methods in Enzymology
https://www.readbyqxmd.com/read/29908935/scale-invariant-rearrangement-of-resting-state-networks-in-the-human-brain-under-sustained-stimulation
#11
Silvia Tommasin, Daniele Mascali, Marta Moraschi, Tommaso Gili, Ibrahim Eid Assan, Michela Fratini, Mauro DiNuzzo, Richard G Wise, Silvia Mangia, Emiliano Macaluso, Federico Giove
Brain activity at rest is characterized by widely distributed and spatially specific patterns of synchronized low-frequency blood-oxygenation level-dependent (BOLD) fluctuations, which correspond to physiologically relevant brain networks. This network behaviour is known to persist also during task execution, yet the details underlying task-associated modulations of within- and between-network connectivity are largely unknown. In this study we exploited a multi-parametric and multi-scale approach to investigate how low-frequency fluctuations adapt to a sustained n-back working memory task...
June 14, 2018: NeuroImage
https://www.readbyqxmd.com/read/29903517/prevalence-of-wild-type-attr-assessed-as-myocardial-uptake-in-bone-scan-in-the-elderly-population
#12
Laroussi Mohamed-Salem, Juan José Santos-Mateo, Juan Sanchez-Serna, Álvaro Hernández-Vicente, Rafael Reyes-Marle, María I Castellón Sánchez, María Antonia Claver-Valderas, Emiliano Gonzalez-Vioque, Francisco J Haro-Del Moral, Pablo García-Pavía, Domingo A Pascual-Figal
BACKGROUND: Myocardial uptake of bone tracers has emerged as useful tool for the early detection of transthyretin amyloidosis (ATTR). The prevalence of wild-type ATTR (ATTRwt) in individuals remains to be established. METHODS: All whole body bone scans performed in individuals ≥ 75 years with no previous clinical suspicion of ATTR were revised in a population-based university hospital over a 7-year period (1509 studies corresponding to 1114 patients; 80...
June 6, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29902415/synthesis-of-newly-functionalized-1-4-naphthoquinone-derivatives-and-their-effects-on-wound-healing-in-alloxan-induced-diabetic-mice
#13
Silvia Helena Cardoso, Cleidijane Rodrigues de Oliveira, Ari Souza Guimarães, Jadiely Nascimento, Julianderson de Oliveira Dos Santos Carmo, Jamylle Nunes de Souza Ferro, Ana Carolina de Carvalho Correia, Emiliano Barreto
Naphthoquinone derivatives have various pharmacological properties. Here, we describe the synthesis of new 1,4-naphthoquinone derivatives inspired by lawsone and β-lapachone and their effects on both migration of fibroblasts in vitro and dermal wound healing in diabetic mice. NMR and FTIR spectroscopy aided characterization of chemical composition and demonstrated the molecular variations after the synthesis of four different derivatives, namely 2-bromo-1,4-naphthoquinone (termed derivative S3), 2-N-phenylamino-1,4-naphthoquinone (derivative S5), 2-N-isonicotinoyl-hydrazide-1,4-naphthoquinone (derivative S6), and 1-N-isonicotinoyl-hydrazone-[2-hydroxy-3-(3-methyl-2-butenyl)]-1,4-naphthoquinone (derivative S7)...
June 11, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29902147/la-cirug%C3%A3-a-tor%C3%A3-cica-video-asistida-vats-temprana-mejora-la-calidad-de-vida-en-los-derrames-pleurales-neopl%C3%A3-sicos-complicados
#14
Facundo Nicolás Alvarez Padilla, Emiliano Nestor Schiavoni, Mario Eduardo Francisco Bustos
INTRODUCCIONEl derrame pleural neoplásico (DPN) implica una enfermedad oncológica avanzada. La biopsia pleural por cirugía torácica endoscópica permite el diagnóstico en más del 90% de los casos y la instrumentación del espacio pleural complicado, mejorando los resultados de la técnica.MATERIAL Y METODOSe realizó un análisis retrospectivo de pacientes con DPN operados para la realización de una pleurodesis química con talco. Se formaron dos grupos, uno con derrame pleural neoplásico complicado (DPNC) y otro con derrame pleural neoplásico no complicado (DPNNC)...
April 20, 2018: Revista de la Facultad de Ciencias Médicas
https://www.readbyqxmd.com/read/29901600/determining-the-burden-of-the-family-caregivers-of-people-with-neuromuscular-diseases-who-use-a-wheelchair
#15
Thais Pousada, Betania Groba, Laura Nieto-Riveiro, Alejandro Pazos, Emiliano Díez, Javier Pereira
The present study provides a basic outline of the care and support that family caregivers offer to people affected by neuromuscular diseases.To determine the presence of burden in caregivers of people with neuromuscular diseases who use a wheelchair and to establish whether the presence of burden is influenced by contextual factors, between them, the use of wheelchair.The applied design was cross-sectional and descriptive. The data were collected through a specific questionnaire, the Functional Independence Measure, the Matching Person and Technology form and the Zarit Burden Interview...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29898200/anthelmintic-effect-of-cassia-fistula-and-combretum-leprosum-protein-fractions-against-goat-gastrointestinal-nematodes
#16
Mara Thais de Oliveira Silva, Mara Andrade Colares Maia, Michele Dalvina Correia da Silva, Taffarel Melo Torres, José Carlos da Silveira Pereira, João Ronielly Campêlo Araújo, Ryan Emiliano da Silva, Anna Lopes da Costa Souza, Tallyson Nogueira Barbosa, Ana Carla Diógenes Suassuna Bezerra
In this study, we evaluated the ovicidal and larvicidal activity of protein preparations obtained from Cassia fistula L. and Combretum leprosum Mart. leaves on the gastrointestinal parasites of goats. Protein preparations were obtained after the extraction of C. fistula L. and C. leprosum Mart. leaves, followed by protein fractionation (with ammonium sulfate saturation percentages of 30%, 30%-60%, and 60%-90%) and dialysis, which resulted in protein fractions (called F1, F2, and F3, respectively). The fractions were evaluated by egg hatching (the eggs were recovered in stool samples from naturally infected goats) and larval development tests...
June 11, 2018: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/29896896/technical-note-extension-of-cerr-for-computational-radiomics-a-comprehensive-matlab-platform-for-reproducible-radiomics-research
#17
Aditya P Apte, Aditi Iyer, Mireia Crispin-Ortuzar, Rutu Pandya, Lisanne V van Dijk, Emiliano Spezi, Maria Thor, Hyemin Um, Harini Veeraraghavan, Jung Hun Oh, Amita Shukla-Dave, Joseph O Deasy
PURPOSE: Radiomics is a growing field of image quantification, but it lacks stable and high-quality software systems. We extended the capabilities of the Computational Environment for Radiological Research (CERR) to create a comprehensive, open-source, MATLAB-based software platform with an emphasis on reproducibility, speed, and clinical integration of radiomics research. METHOD: The radiomics tools in CERR were designed specifically to quantify medical images in combination with CERR's core functionalities of radiological data import, transformation, management, image segmentation, and visualization...
June 13, 2018: Medical Physics
https://www.readbyqxmd.com/read/29894354/very-late-migration-to-and-retrieval-of-an-amplatzer-patent-foramen-ovale-occlusion-device-from-the-abdominal-aortic-carrefour
#18
Gerardo Musuraca, Pierfrancesco Agostoni, Emiliano Boldi, Ferdinando Imperadore, Clotilde Terraneo, Giovanni P Broso
: The migration of a percutaneous patent foramen ovale closure device is a very rare but serious complication. Late or very late embolization of device requires a different management strategy due to the inability of the device to deform to allow passage into a large caliber sheath compared with the early, periprocedural device migration. This last complication can normally be managed with snare and percutaneous retrieval.
June 11, 2018: Journal of Cardiovascular Medicine
https://www.readbyqxmd.com/read/29893773/to-move-or-not-to-move-functional-role-of-ventral-premotor-cortex-in-motor-monitoring-during-limb-immobilization
#19
Francesca Garbarini, Luca Cecchetti, Valentina Bruno, Angela Mastropasqua, Carlotta Fossataro, Giuseppe Massazza, Katiuscia Sacco, Maria Consuelo Valentini, Emiliano Ricciardi, Anna Berti
Anatomo-clinical evidence from motor-awareness disorders after brain-damages suggests that the premotor cortex (PMC) is involved in motor-monitoring of voluntary actions. Indeed, PMC lesions prevent patients from detecting the mismatch between intended, but not executed, movements with the paralyzed limb. This functional magnetic resonance imaging study compared, in healthy subjects, free movements against blocked movements, precluded by a cast. Cast-related corticospinal excitability changes were investigated by using transcranial magnetic stimulation...
June 11, 2018: Cerebral Cortex
https://www.readbyqxmd.com/read/29893041/culturally-and-linguistically-diverse-oncology-patients-perspectives-of-consultation-audio-recordings-and-question-prompt-lists
#20
Amelia Hyatt, Ruby Lipson-Smith, Karla Gough, Phyllis Butow, Michael Jefford, Thomas F Hack, Sandra Hale, Emiliano Zucchi, Shane White, Uldis Ozolins, Penelope Schofield
OBJECTIVE: Ethnicity and migrant status results in disparities with cancer burden and survival; with communication difficulties cited as the main barrier to access. Our research team tested a communication intervention package comprising consultation audio-recordings (ARs) and question prompt lists (QPLs) for Low English Speaking (LES) patients with cancer. This study explored LES patient experiences, preferences, and recommendations regarding the communication package. METHODS: Participants completed a questionnaire and qualitative interview regarding ARs and QPLs...
June 11, 2018: Psycho-oncology
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