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https://www.readbyqxmd.com/read/29284022/a-transcriptomics-approach-uncovers-novel-roles-for-poly-adp-ribosyl-ation-in-the-basal-defense-response-in-arabidopsis-thaliana
#1
Amy G Briggs, Lori C Adams-Phillips, Brian D Keppler, Sophia G Zebell, Kyle C Arend, April A Apfelbaum, Joshua A Smith, Andrew F Bent
Pharmacological inhibition of poly(ADP-ribose) polymerase (PARP) or loss of Arabidopsis thaliana PARG1 (poly(ADP-ribose) glycohydrolase) disrupt a subset of plant defenses. In the present study we examined the impact of altered poly(ADP-ribosyl)ation on early gene expression induced by the microbe-associate molecular patterns (MAMPs) flagellin (flg22) and EF-Tu (elf18). Stringent statistical analyses and filtering identified 178 genes having MAMP-induced mRNA abundance patterns that were altered by either PARP inhibitor 3-aminobenzamide (3AB) or PARG1 knockout...
2017: PloS One
https://www.readbyqxmd.com/read/29187017/anti-apoptotic-effect-of-3-aminobenzamide-an-inhibitor-of-poly-adp-ribose-polymerase-against-multiple-organ-damage-induced-by-gamma-irradiation-in-rats
#2
Marwa M El-Sheikh, Rania M El-Hazek, Aiman S El-Khatib, Mona A El-Ghazaly
PURPOSE: This study aimed to investigate the effect of 3-aminobenzamide (3-AB) in doses of 5, 10 and 15 mg/kg on the inhibition of Poly (ADP-ribose) polymerase (PARP) when combined with ionizing radiation (IR). MATERIAL AND METHODS: Rats were treated intraperitonealy, 1 hour prior to irradiation at a dose level of 6 Gray (Gy) and were sacrificed 24 hours after irradiation. Control groups were run concurrently. RESULTS: IR led to an increase of thiobarbituric acid reactive substance (TBARS), nitrite as well as a decrease in total antioxidant capacity associated increase in myeloperoxidase (MPO) with expression of cyclooxygenase-2 (COX-2)...
November 30, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29137380/inhibitors-of-telomerase-and-poly-adp-ribose-polymerases-synergize-to-limit-the-lifespan-of-pancreatic-cancer-cells
#3
Katrina M Burchett, Asserewou Etekpo, Surinder K Batra, Ying Yan, Michel M Ouellette
Imetelstat (GRN163L) is a potent and selective inhibitor of telomerase. We have previously reported that GRN163L could shorten telomeres and limit the lifespan of CD18/HPAF and CAPAN1 pancreatic cancer cells. Here, we examined the effects of GRN163L on two other pancreatic cancer cell lines: AsPC1 and L3.6pl. In both lines, chronic exposure to GRN163L led to an initial shortening of telomeres followed by a stabilization of extremely short telomeres. In AsPC1 cells, telomere attrition eventually led to the induction of crisis and the loss of the treated population...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29051731/parp-1-and-p53-regulate-the-increased-susceptibility-to-oxidative-death-of-lymphocytes-from-mci-and-ad-patients
#4
Felipe Salech, Daniela P Ponce, Carol D SanMartín, Nicole K Rogers, Carlos Chacón, Mauricio Henríquez, Maria I Behrens
Mild cognitive impairment (MCI) is a clinically detectable initial stage of cognitive deterioration with a high conversion rate to dementia. There is increasing evidence that some of the cerebral alterations present in Alzheimer type dementia can be found in peripheral tissues. We have previously shown that lymphocytes from Alzheimer's disease (AD) patients have increased susceptibility to hydrogen peroxide (H2O2)-induced death that depends on dementia severity. We here investigated whether lymphocytes from MCI patients show increased vulnerability to death, and explored the involvement of Poly [ADP-ribose] polymerase (PARP-1) and p53 in the regulation of this process...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/29016792/antidepressant-like-actions-of-inhibitors-of-poly-adp-ribose-polymerase-in-rodent-models
#5
Gregory A Ordway, Attila Szebeni, Liza J Hernandez, Jessica D Crawford, Katalin Szebeni, Michelle J Chandley, Katherine C Burgess, Corwin Miller, Erol Bakkalbasi, Russell W Brown
Background: Many patients suffering from depressive disorders are refractory to treatment with currently available antidepressant medications, while many more exhibit only a partial response. These factors drive research to discover new pharmacological approaches to treat depression. Numerous studies demonstrate evidence of inflammation and elevated oxidative stress in major depression. Recently, major depression has been shown to be associated with elevated levels of DNA oxidation in brain cells, accompanied by increased gene expression of the nuclear base excision repair enzyme, poly(ADP-ribose) polymerase-1...
August 3, 2017: International Journal of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28780454/nicotinamide-antioxidative-and-dna-hypomethylation-effects-in-plant-cells
#6
Torkel Berglund, Anders Wallström, Tuong-Van Nguyen, Cecilia Laurell, Anna B Ohlsson
The effects of nicotinamide (NIC) and its natural plant metabolites nicotinic acid (NIA) and trigonelline (TRIG) were studied with respect to defense in plant cell cultures. NIC and NIA could protect against oxidative stress damage caused by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), which generates free radicals. Damage was analyzed as DNA strand breaks in cell cultures of Pisum sativum (garden pea), Daucus carota (carrot), Populus tremula L. × P. tremuloides (hybrid aspen) and Catharanthus roseus (Madagascar periwinkle), monitored by single cell gel electrophoresis (comet assay), and assays of cell leakage in C...
July 29, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28753579/inhibitors-of-telomerase-and-poly-adp-ribose-polymerases-synergize-to-limit-the-lifespan-of-pancreatic-cancer-cells
#7
Katrina M Burchett, Asserewou Etekpo, Surinder K Batra, Ying Yan, Michel M Ouellette
Imetelstat (GRN163L) is a potent and selective inhibitor of telomerase. We have previously reported that GRN163L could shorten telomeres and limit the lifespan of CD18/HPAF and CAPAN1 pancreatic cancer cells. Here, we examined the effects of GRN163L on two other pancreatic cancer cell lines: AsPC1 and L3.6pl. In both lines, chronic exposure to GRN163L led to an initial shortening of telomeres followed by a stabilization of extremely short telomeres. In AsPC1 cells, telomere attrition eventually led to the induction of crisis and the loss of the treated population...
July 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28695522/purification-of-recombinant-human-parp-3
#8
Jean-Christophe Amé, Barbara Camuzeaux, Françoise Dantzer, Valérie Schreiber
The purification of poly(ADP-ribose) polymerase-3 (PARP-3) from overexpressing cells (Sf9 insect cells, Escherichia coli) has been updated to a fast and reproducible two chromatographic steps protocol. After cell lysis, PARP-3 protein from the crude extract is affinity purified on a 3-aminobenzamide Sepharose™ chromatographic step. The last contaminants and the 3-methoxybenzamide used to elute PARP-3 from the previous affinity column are removed on the high-performance strong cations exchanger MonoQ™ matrix...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28676262/adaptive-response-in-mouse-bone-marrow-stromal-cells-exposed-to-900mhz-radiofrequency-fields-impact-of-poly-adp-ribose-polymerase-parp
#9
Qina He, Lin Zong, Yulong Sun, Vijayalaxmi, Thomas J Prihoda, Jian Tong, Yi Cao
This study examined whether non-ionizing radiofrequency fields (RF) exposure is capable of inducing poly (ADP-ribose) polymerase-1 (PARP-1) in bone marrow stromal cells (BMSCs) and whether it plays a role in RF-induced adaptive response (AR). Bone marrow stromal cells (BMSCs) were exposed to 900MHz RF at 120μW/cm(2) power flux density for 3h/day for 5days and then challenged with a genotoxic dose of 1.5Gy gamma-radiation (GR). Some cells were also treated with 3-aminobenzamide (3-AB, 2mM final concentration), a potent inhibitor of PARP-1...
August 2017: Mutation Research
https://www.readbyqxmd.com/read/28656263/rho-kinase-signaling-pathway-promotes-the-expression-of-parp-to-accelerate-cardiomyocyte-apoptosis-in-ischemia-reperfusion
#10
Hongjun Bian, Yi Zhou, Bin Yu, Deya Shang, Fuli Liu, Bin Li, Jianni Qi
It has been previously reported that Rho‑kinase (ROCK) and poly ADP-ribose polymerase (PARP) serve critical roles in myocardial ischemia/reperfusion (I/R) injury. Studies have additionally demonstrated that the activation of ROCK and the expression of PARP are increased in I/R. However, the effect and mechanism of the two proteins remains to be fully elucidated in I/R. In addition, whether they can be influenced by each other is unclear. In the present study, it was demonstrated that ischemia followed by reperfusion resulted in a significant increase in ROCK and PARP...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28322842/simultaneous-blockade-of-nmda-receptors-and-parp-1-activity-synergistically-alleviate-immunoexcitotoxicity-and-bioenergetics-in-3-nitropropionic-acid-intoxicated-mice-evidences-from-memantine-and-3-aminobenzamide-interventions
#11
Saravana Babu Chidambaram, Ranju Vijayan, Sathiya Sekar, Sugumar Mani, Barathidsan Rajamani, Ramakrishnan Ganapathy
Interlink between excitotoxicity and cellular bioenergetics depletion is implicated as one of the central deteriorative pathways in many neurodegenerative diseases including Huntington's disease (HD). Chronic administration of 3-nitropropionic acid (3-NP) depletes ATP and NAD(+;) and increases TNFα, IL-6 and glutamate content resulting in "immunoexcitotoxicity". Present study was designed to determine whether the combination of memantine (MN) and 3-aminobenzamide (3-AB), PARP inhibitor, can ameliorate immunoexcitotoxicity and improve bioenergetics in a better manner than individual administration against 3-NP intoxication in mice...
May 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28257805/jnk1-and-jnk3-play-a-significant-role-in-both-neuronal-apoptosis-and-necrosis-evaluation-based-on-in-vitro-approach-using-tert-butylhydroperoxide-induced-oxidative-stress-in-neuro-2a-cells-and-perturbation-through-3-aminobenzamide
#12
Vijaya Prakash Krishnan Muthaiah, Felicia Mary Michael, Tamilselvi Palaniappan, Sridhar Skylab Rajan, Kirubhanand Chandrasekar, Sankar Venkatachalam
In spinal cord injury (SCI), oxidative stress in the penumbra of the injury site is a characteristic feature. The predominance of necrosis over apoptosis in the ensuing delayed cell death results in progressive waves of necrosis affecting neighboring cells and thus exaggerates the severity of the lesion. Necrosis has been classified into subtypes based on the active molecular players and parthanatos is one among them, which is characterized by the over activation of PARP1 as the pre-mitochondrial event that triggers necrosis...
February 28, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27965086/the-effect-of-poly-adp-ribosyl-ation-inhibition-on-the-porcine-cumulus-oocyte-complex-during-in%C3%A2-vitro-maturation
#13
Duk Hyoun Kim, Hye Ran Lee, Min Gyeong Kim, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
Poly(ADP-ribosyl)ation (PARylation) plays important roles in DNA repair, apoptosis, transcriptional regulation, and cell death, and occurs via the activity of poly(ADP-ribose) polymerases (PARPs). Previous studies have shown that PARylation affects mouse and porcine pre-implantation development and participates in mechanisms of autophagy. However, there have not yet been reported the role of PARylation during in vitro maturation (IVM) of porcine oocytes. Thus, we investigated the effect of PARylation inhibition on this process; cumulus-oocyte complexes (COCs) were cultured with 3-aminobenzamide (3-ABA, PARP inhibitor) during porcine IVM...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27722032/parp1-differentially-interacts-with-promoter-region-of-dux4-gene-in-fshd-myoblasts
#14
Vishakha Sharma, Sachchida Nand Pandey, Hunain Khawaja, Kristy J Brown, Yetrib Hathout, Yi-Wen Chen
OBJECTIVE: The goal of the study is to identity proteins, which interact with the promoter region of double homeobox protein 4 (DUX4) gene known to be causative for the autosomal dominant disorder Facioscapulohumeral Muscular Dystrophy (FSHD). METHODS: We performed a DNA pull down assay coupled with mass spectrometry analysis to identify proteins that interact with a DUX4 promoter probe in Rhabdomyosarcomca (RD) cells. We selected the top ranked protein poly (ADP-ribose) polymerase 1 (PARP1) from our mass spectrometry data for further ChIP-qPCR validation using patients' myoblasts...
August 2016: Journal of Genetic Syndrome & Gene Therapy
https://www.readbyqxmd.com/read/27686254/the-poly-adp-ribosyl-ation-of-foxo3-mediated-by-parp1-participates-in-isoproterenol-induced-cardiac-hypertrophy
#15
Jing Lu, Renwei Zhang, Huiqi Hong, Zuolong Yang, Duanping Sun, Shuya Sun, Xiaolei Guo, Jiantao Ye, Zhuoming Li, Peiqing Liu
The Forkhead box-containing protein, O subfamily 3 (FoxO3) transcription factor negatively regulates myocardial hypertrophy, and its transcriptional activity is finely conditioned by diverse posttranslational modifications, such as phosphorylation, acetylation, ubiquitination, methylation and glycosylation. Here, we introduce a novel modification of the FoxO3 protein in cardiomyocytes: poly(ADP-ribosyl)ation (PARylation) mediated by poly(ADP-ribose) polymerase-1 (PARP1). This process catalyzes the NAD(+)-dependent synthesis of polymers of ADP-ribose (PAR) and their subsequent attachment to target proteins by PARPs...
December 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27604227/parp-inhibition-treatment-in-a-nonconventional-experimental-mouse-model-of-chronic-asthma
#16
Raffaela Zaffini, Rosanna Di Paola, Salvatore Cuzzocrea, Marta Menegazzi
Allergic asthma is an immunological disease that occurs as a consequence of aeroallergen exposure. Inhibition of poly(ADP-ribose) polymerases (PARPs) in conventional models of asthma-like reaction has emerged as an effective anti-inflammatory and airway remodeling intervention. In a house dust mite (HDM) exposure mouse model, we investigated the impact of PARP inhibition on allergic airway inflammation, sensitization, and remodeling. Mice were intranasally exposed to a HDM extract for 5 days per week for up to 5 weeks...
December 2016: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/27465581/poly-adp-ribose-polymerase-inhibition-suppresses-inflammation-and-promotes-recovery-from-adrenal-injury-in-a-rat-model-of-acute-necrotizing-pancreatitis
#17
Jia Yu, Teng Zuo, Wenhong Deng, Qiao Shi, Peng Ma, Chen Chen, Liang Zhao, Kailiang Zhao, Weixing Wang
BACKGROUND: Poly(ADP-ribose) polymerase (PARP) participates in multi-organ failure in various inflammatory diseases including acute necrotizing pancreatitis (ANP). Since pancreatitis-associated adrenal insufficiency is partly caused by inflammatory damage to the adrenal cortex, we examined whether PARP antagonism could alleviate adrenal insufficiency in a rat model of ANP. METHODS: ANP was induced by retrograde infusion of sodium taurocholate into the bile-pancreatic duct...
July 27, 2016: BMC Gastroenterology
https://www.readbyqxmd.com/read/27382509/poly-adp-ribose-polymerase-regulates-glycolytic-activity-in-kidney-proximal-tubule-epithelial-cells
#18
Hana Song, Sang Pil Yoon, Jinu Kim
After renal injury, selective damage occurs in the proximal tubules as a result of inhibition of glycolysis. The molecular mechanism of damage is not known. Poly(ADP-ribose) polymerase (PARP) activation plays a critical role of proximal tubular cell death in several renal disorders. Here, we studied the role of PARP on glycolytic flux in pig kidney proximal tubule epithelial LLC-PK1 cells using XFp extracellular flux analysis. Poly(ADP-ribosyl)ation by PARP activation was increased approximately 2-fold by incubation of the cells in 10 mM glucose for 30 minutes, but treatment with the PARP inhibitor 3-aminobenzamide (3-AB) does-dependently prevented the glucose-induced PARP activation (approximately 14...
June 2016: Anatomy & Cell Biology
https://www.readbyqxmd.com/read/27331361/pharmacological-reconditioning-of-marginal-donor-rat-lungs-using-inhibitors-of-peroxynitrite-and-poly-adp-ribose-polymerase-during-ex-vivo-lung-perfusion
#19
Xingyu Wang, Yabo Wang, Roumen Parapanov, Etienne Abdelnour, Fabrizio Gronchi, Jean Yannis Perentes, Lise Piquilloud, Hans-Beat Ris, Igor Letovanec, Lucas Liaudet, Thorsten Krueger
BACKGROUND: Donor lungs obtained after prolonged warm ischemia (WI) may be unsuitable for transplantation due to the risk of reperfusion injury, but could be reconditioned using ex-vivo lung perfusion (EVLP). Key processes of reperfusion injury include the formation of reactive oxygen species (ROS)/nitrogen species (RNS) and the activation of poly(adenosine diphosphate-ribose) polymerase (PARP). We explored whether rat lungs obtained after WI could be reconditioned during EVLP using the ROS/RNS scavenger Mn(III)-tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) or the PARP inhibitor 3-aminobenzamide (3-AB)...
July 2016: Transplantation
https://www.readbyqxmd.com/read/27271689/poly-adp-ribose-polymerase-inhibition-provides-protection-against-lung-injury-in-a-rat-paraquat-toxicity-model
#20
Salim Kemal Tuncer, Seher Altinel, Mehmet Toygar, Hakan Istanbulluoglu, Kahraman Ates, Recai Ogur, Ozcan Altinel, Yildirim Karslioglu, Turgut Topal, Ahmet Korkmaz, Bulent Uysal
OBJECTIVES: Paraquat (PQ) is a widely used herbicide. Exposure to PQ at toxic doses can result in fatal acute lung injury. Inhibition of the poly-(ADP-ribose) polymerase (PARP) enzyme alleviates inflammation and necrosis in various pathologies. Here we aimed to evaluate the effects of PARP inhibition on PQ-induced lung damage in a rat experimental model. METHODS: Female Sprague-Dawley rats (n = 24) were allocated into three groups: sham, PQ and PQ + 3-aminobenzamide (3-AB) that is a PARP inhibitor, groups...
August 2016: Inflammopharmacology
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