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https://www.readbyqxmd.com/read/29692616/il-21-secreting-hucmscs-combined-with-mir-200c-inhibit-tumor-growth-and-metastasis-via-repression-of-wnt-%C3%AE-catenin-signaling-and-epithelial-mesenchymal-transition-in-epithelial-ovarian-cancer
#1
Yunxia Zhang, Jing Wang, Di Wu, Miao Li, Fenshu Zhao, Mulan Ren, Yunlong Cai, Jun Dou
Background: Epithelial ovarian cancer (EOC) with insidious characteristic manifests no symptoms in its early onset but most patients have advanced and distant cancer metastasis at diagnosis. Innovative early diagnosis and effective treatment of EOC are urgently needed. Methods: In the study, we developed a novel agent of IL-21-secreting human umbilical cord mesenchymal stem cells (hUCMSCs) combined with miR-200c to evaluate its effects on SKOV3 EOC in vitro and in vivo...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29691553/-klf10-silencing-inhibited-mechanical-force-induced-osteogenic-differentiation-of-human-periodontal-ligament-cells
#2
Xiao-Qin Yu, Yun-Long Li, Jin-Yu Gao, Hua Liu
PURPOSE: To investigate the effect of Klf10 silence on human periodontal ligament cells (HPDLCs) under mechanical force. METHODS: HPDLCs were isolated and transfected with Klf10 siRNA, and then exposed to centrifugal force for 6 h at 631 r/min. Purmorphamine, an hedgehog signaling pathway agonist, was used for intervention. The activity of alkaline phosphatase (ALP) was detected by ELISA. RT-PCR and Western blot were performed to detect the mRNA and protein expression of Klf10, Runt related transcription factor 2 (Runx2), osteopontin (OPN) and osteocalcin (OCN)...
December 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29690664/-clinicopathologic-features-of-gastric-plexiform-fibromyxoma
#3
G M Hu, H P Chen, Q Y Liu, X C Shi, H F Wu, Y K Feng, J L Ren, C F Wang
Objective: To analyse the clinicopathologic features of gastric plexiform fibromyxoma (PF) including diagnosis, differential diagnosis, immunohistochemistry and molecular pathology. Methods: Eight cases of PF were collected from June 2006 to June 2017 at the Second Affiliated Hospital of Zhengzhou University and the First Affiliated Hospital of Zhengzhou University. The clinicopathologic findings of eight cases of PF were retrospectively analyzed, and immunohistochemistry (EnVision method) and molecular detection of glioma-associated oncogene homologue 1 (GLI1) gene translocation were performed...
April 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/29688404/heparan-sulfate-proteoglycan-sulfation-regulates-uterine-differentiation-and-signaling-during-embryo-implantation
#4
Yan Yin, Adam Wang, Li Feng, Yu Wang, Hong Zhang, Ivy Zhang, Brent Bany, Liang Ma
To prepare for embryo implantation, uterus must undergo a series of reciprocal interactions between the uterine epithelium and the underlying stroma, which are orchestrated by ovarian hormones. During this process, multiple signaling pathways are activated to direct cell proliferation and differentiation, which render the uterus receptive to the implanting blastocysts. One important modulator of these signaling pathways is the cell surface and extracellular matrix macromolecules, heparan sulfate proteoglycans (HSPGs)...
April 23, 2018: Endocrinology
https://www.readbyqxmd.com/read/29687851/mir-132-weakens-proliferation-and-invasion-of-glioma-cells-via-the-inhibition-of-gli1
#5
Y-Z Wang, J-J Han, S-Q Fan, W Yang, Y-B Zhang, T-J Xu, G-M Xu
OBJECTIVE: Transcriptional factor Gli1 in Hedgehog signal pathway facilitates epithelial mesenchymal transition (EMT) and is associated with invasion or proliferation of multiple tumor cells. The previous study showed the correlation between miR-132 down-regulation and glioma pathogenesis. We investigated the role of miR-132 in mediating Gli1 expression and in affecting proliferation or invasion of glioma cells. PATIENTS AND METHODS: Dual luciferase reporter gene assay was used to confirm the targeted regulation between miR-132 and Gli1...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29665144/disulfiram-copper-targets-stem-cell-like-aldh-population-of-multiple-myeloma-by-inhibition-of-aldh1a1-and-hedgehog-pathway
#6
Na Jin, Xiaojian Zhu, Fanjun Cheng, Liling Zhang
Multiple myeloma stem cells (MMSCs) have been considered as the major cause resulting in relapse. Eradicating MMSCs may be an effective strategy to improve the outcome of multiple myeloma (MM). Increased activity of aldehyde dehydrogenase (ALDH) has been found in MMSCs, but whether inhibiting ALDH activity can eliminate MMSCs remains unknown. Disulfiram (DS) has been reported as an inhibitor of ALDH, and increasing studies showed it has anti-cancer effects in a copper (Cu)-dependent manner. In this study, we isolated ALDH+ cells of MM by Aldefluor assay and demonstrated they possessed tumorigenesis capacities in vitro and in vivo...
April 17, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29662197/mekk2-and-mekk3-suppress-hedgehog-pathway-dependent-medulloblastoma-by-inhibiting-gli1-function
#7
Jinqiu Lu, Liansheng Liu, Mingjie Zheng, Xiaoling Li, Ailing Wu, Qingzhe Wu, Cheng Liao, Jian Zou, Hai Song
Hedgehog (Hh) pathway plays a pivotal role in diverse aspects of development and postnatal physiology. Perturbation of Hh signaling and activation of GLI1 (glioma-associated oncogene 1), a dedicated transcription factor for Hh pathway, are highly associated with several cancers, such as medulloblastoma and basal cell carcinoma. Dynamic and precise control of GLI1 activity is thus important to ensure proper homeostasis and tumorigenesis. Here we show that MEKK2 (MAP3K2) and MEKK3 (MAP3K3) inhibit GLI1 transcriptional activity and oncogenic function through phosphorylation on multiple Ser/Thr sites of GLI1, which reduces GLI1 protein stability, DNA-binding ability, and increases the association of GLI1 with SUFU...
April 17, 2018: Oncogene
https://www.readbyqxmd.com/read/29659833/ift172-conditional-knockout-mice-exhibit-rapid-retinal-degeneration-and-protein-trafficking-defects
#8
Priya R Gupta, Nachiket Pendse, Scott H Greenwald, Mihoko Leon, Qin Liu, Eric A Pierce, Kinga M Bujakowska
Intraflagellar transport (IFT) is a bidirectional transport process that occurs along primary cilia and specialized sensory cilia, such as photoreceptor outer-segments. Genes coding for various IFT components are associated with ciliopathies. Mutations in IFT172 lead to diseases ranging from isolated retinal degeneration to severe syndromic ciliopathies. In this study, we created a mouse model of IFT172-associated retinal degeneration to investigate the ocular disease mechanism. We found that depletion of IFT172 in rod photoreceptors leads to a rapid degeneration of the retina, with severely reduced electroretinography responses by one month and complete outer-nuclear layer degeneration by two months...
April 5, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29659575/synergistic-co-regulation-and-competition-by-a-sox9-gli-foxa-phasic-transcriptional-network-coordinate-chondrocyte-differentiation-transitions
#9
Zhijia Tan, Ben Niu, Kwok Yeung Tsang, Ian G Melhado, Shinsuke Ohba, Xinjun He, Yongheng Huang, Cheng Wang, Andrew P McMahon, Ralf Jauch, Danny Chan, Michael Q Zhang, Kathryn S E Cheah
The growth plate mediates bone growth where SOX9 and GLI factors control chondrocyte proliferation, differentiation and entry into hypertrophy. FOXA factors regulate hypertrophic chondrocyte maturation. How these factors integrate into a Gene Regulatory Network (GRN) controlling these differentiation transitions is incompletely understood. We adopted a genome-wide whole tissue approach to establish a Growth Plate Differential Gene Expression Library (GP-DGEL) for fractionated proliferating, pre-hypertrophic, early and late hypertrophic chondrocytes, as an overarching resource for discovery of pathways and disease candidates...
April 16, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29658573/hedgehog-signaling-regulates-the-expression-levels-of-inflammatory-mediators-in-cigarette%C3%A2-induced-airway-inflammation
#10
Yi Guo, Guochao Shi, Huanying Wan, Min Zhou
Chronic obstructive pulmonary disease (COPD) is a persistent airway inflammation influenced by cigarette smoke. Previous studies have reported that Hedgehog (Hh) signaling is aberrantly activated by cigarette smoke and dysregulated in COPD. The present study explored the role of the Hh signaling pathway on the expression levels of certain inflammatory mediators in cigarette‑induced airway inflammation. Herein, a total of three A549 cell populations were generated: The A0 group as control cells, the A1 group cells treated with nicotine at a concentration of 10 µM for 12, 24 and 48 h, and the A2 group cultured simultaneously with nicotine and cyclopamine for the same duration...
April 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29653361/lncrna-asap1-it1-positively-modulates-the-development-of-cholangiocarcinoma-via-hedgehog-signaling-pathway
#11
Linqi Guo, Yu Zhou, Ying Chen, Huawei Sun, Yue Wang, Yikun Qu
Over the past decades, lncRNAs have attracted more and more attentions of researchers. It has been verified that lncRNAs can modulate multiple biological behaviors in various human cancers. LncRNA ASAP1-IT1 has been certified to be a tumor facilitator in several malignant tumors. This study aims to investigate the effects of dysregulated ASAP1-IT1 on biological processes of Cholangiocarcinoma. The high expression level of ASAP1-IT1 was tested in Cholangiocarcinoma tissues and cells with qRT-PCR. Upregulation of ASAP1-IT predicted the unfavorable prognosis for Cholangiocarcinoma patients...
April 10, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29628779/role-and-inhibition-of-gli1-protein-in-cancer
#12
REVIEW
Eloise Mastrangelo, Mario Milani
GLI1 is a transcriptional regulator involved in the development of different types of cancer. GLI1 transcriptional activity is regulated within the Hedgehog pathway (canonical activity), but can also be controlled independently (non-canonical activity) in the context of other signaling pathways. Experimental evidences show GLI1 involvement in both small- and non-small-cell lung cancers. Direct inhibition of the protein, in combination with other chemotherapeutic agents, represents a promising strategy for the treatment of different malignancies...
2018: Lung Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/29627579/genetic-detection-of-sonic-hedgehog-shh-expression-and-cellular-response-in-the-progression-of-acute-through-chronic-demyelination-and-remyelination
#13
Maria A Sanchez, Genevieve M Sullivan, Regina C Armstrong
Multiple sclerosis is a demyelinating disease in which neurological deficits result from damage to myelin, axons, and neuron cell bodies. Prolonged or repeated episodes of demyelination impair remyelination. We hypothesized that augmenting Sonic hedgehog (Shh) signaling in chronically demyelinated lesions could enhance oligodendrogenesis and remyelination. Shh regulates oligodendrocyte development during postnatal myelination, and maintains adult neural stem cells. We used genetic approaches to detect Shh expression and Shh responding cells in vivo...
April 6, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29626476/long-non-coding-rna-thor-promotes-human-osteosarcoma-cell-growth-in-vitro-and-in-vivo
#14
Wangzhen Chen, Meikai Chen, Yifan Xu, Xuerong Chen, Ping Zhou, Xiaofeng Zhao, Fei Pang, Wenqing Liang
Long non-coding RNA (LncRNA) dysregulation is associated with human osteosarcoma (OS) cell progression. Recent studies have characterized a novel but ultra-conserved LncRNA THOR ("Lnc-THOR") as a cancer-specific LncRNA, mediating cell growth. In the current study, we show that Lnc-THOR is expressed in established and primary human OS cells. It is also detected in human OS tissues, but not in the surrounding normal bone tissues. siRNA-induced knockdown or CRSIPR/Cas9-mediated knockout Lnc-THOR significantly inhibited human OS cell survival and proliferation...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29622661/isolation-of-a-multipotent-mesenchymal-stem-cell-like-population-from-human-adrenal-cortex
#15
Earn Gan, Wendy Robson, Peter Murphy, Robert Pickard, Simon Pearce, Rachel Oldershaw
BACKGROUND: The highly plastic nature of adrenal cortex suggests the presence of adrenocortical stem cells (ACSC) but the exact in vivo identity of ACSC remains elusive. A few studies have demonstrated the differentiation of adipose or bone marrow-derived mesenchymal stem cells (MSC) into steroid-producing cells. We therefore investigated the isolation of multipotent MSC from human adrenal cortex. METHODS: Human adrenals were obtained as discarded surgical material...
April 5, 2018: Endocrine Connections
https://www.readbyqxmd.com/read/29601828/identification-of-variants-in-ret-and-ihh-pathway-members-in-a-large-family-with-history-of-hirschsprung-disease
#16
Y Sribudiani, R K Chauhan, M M Alves, L Petrova, E Brosens, C Harrison, T Wabbersen, B M de Graaf, T Rügenbrink, G Burzynski, R W W Brouwer, W F J van IJcken, S M Maas, A de Klein, J Osinga, B J L Eggen, A J Burns, A S Brooks, I T Shepherd, R M W Hofstra
BACKGROUND & AIMS: Hirschsprung disease (HSCR) is an inherited congenital disorder characterized by absence of enteric ganglia in the distal part of the gut. Variants in ret proto-oncogene (RET) have been associated with up to 50% of familial and 35% of sporadic cases. We searched for variants that affect disease risk in a large, multi-generational family with history of HSCR in a linkage region previously associated with the disease (4q31.3-q32.3) and exome wide. METHODS: We performed exome sequencing analyses of a family in the Netherlands with 5 members diagnosed with HSCR and 2 members diagnosed with functional constipation...
March 27, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29596399/bioinformatic-analysis-of-gli1-and-related-signaling-pathways-in-chemosensitivity-of-gastric-cancer
#17
Tao Yu, Wenzhuo Jia, Qi An, Xianglong Cao, Gang Xiao
BACKGROUND This study assessed the prognostic value of GLI1 in gastric cancer and analyzed the possible GLI1-related signaling network in chemosensitivity. MATERIAL AND METHODS Bioinformatic data mining was performed by using data in the TCGA-Stomach Cancer (TCGA-STAD) and the Kaplan-Meier plotter. GLI1 co-expressed genes in TCGA-STAD were subjected to KEGG pathway analysis. The genes enriched in the KEGG pathways were further subjected to Protein-Protein Interaction (PPI) analysis. RESULTS In TCGA-STAD, high GLI1 gene/exon expression was associated with significantly worse survival (p=0...
March 29, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29581853/the-protective-autophagy-activated-by-gant-61-in-mycn-amplified-neuroblastoma-cells-is-mediated-by-perk
#18
Jing Wang, Siqi Huang, Ruicheng Tian, Jing Chen, Hongxiang Gao, Chenjie Xie, Yuhua Shan, Zhen Zhang, Song Gu, Min Xu
The proto-oncogene MYC can trigger the unfolded protein response (UPR). The double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase (PERK), one of three primary branches of the UPR, is a key regulator of autophagy, promoting tumorigenesis. Upon activation of PERK, there is an increase in phosphorylation of the eukaryotic initiation factor-2 alpha (eIF2α), which in turn, activates the transcription factor-4 (ATF4), responsible for an increased expression of LC3, a common autophagy marker...
March 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29573464/pseudopod-associated-protein-kif20b-promotes-gli1-induced-epithelial-mesenchymal-transition-modulated-by-pseudopodial-actin-dynamic-in-human-colorectal-cancer
#19
Wen-Feng Lin, Xiao-Lu Lin, Seng-Wang Fu, Li Yang, Chao-Tao Tang, Yun-Jie Gao, Hao-Yan Chen, Zhi-Zheng Ge
Kinesin family member 20B (KIF20B) has been reported to have an oncogenic role in bladder and hepatocellular cancer cells, but its role in colorectal cancer (CRC) progression remains unclear. In this study, we assessed the mRNA and protein levels of KIF20B in CRC tissues using qRT-PCR and immunohistochemistry, respectively. KIF20B was overexpressed in CRC tissues and was associated with cancer invasion and metastasis. Mechanistically, KIF20B overexpression promoted the epithelial-mesenchymal transition (EMT) process mediated by glioma-associated oncogene 1 (Gli1) as well as CRC cell migration and invasion...
March 24, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29572896/ass1-and-peri-portal-gene-expression-in-sonic-hedgehog-hepatocellular-adenomas
#20
Jean-Charles Nault, Gabrielle Couchy, Stefano Caruso, Léa Meunier, Laure Caruana, Eric Letouzé, Sandra Rebouissou, Valérie Paradis, Julien Calderaro, Jessica Zucman-Rossi
INTRODUCTION: Genetic alterations define different molecular subclasses of hepatocellular adenoma (HCA) linked with risk factors, histology and clinical behavior. Recently, ASS1, a major peri-portal protein, was proposed as a marker of HCA with a high risk of hemorrhage. We aimed to assess the significance of ASS1 expression through the scope of the HCA molecular classification. METHODS: ASS1 expression was evaluated using RNAseq, quantitative RT-PCR and Immunohistochemistry...
March 24, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
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