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https://www.readbyqxmd.com/read/28726187/best-practices-for-media-selection-for-mammalian-cells
#1
REVIEW
Paul J Price
Cell culture medium is a complex mixture of nutrients and growth factors that, along with the physical environment, can either help or destroy your experiment or production run. Nutritional requirements differ with different cell types and functions, as do optimal pH and osmolality. As cell growth proceeds, different cells will utilize amino acids and other components at different rates. By controlling for ammonia, free radicals, heavy metal toxicity, pH shifts, fluctuations in osmolality, nutrient depletion, and chemical and biological contaminants, you will optimize the chances of success...
July 19, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28726174/comparative-study-of-the-effects-of-a-small-scale-trout-farm-on-the-macrozoobenthos-potamoplankton-and-epilithic-diatom-communities
#2
K Stojanović, M Živić, Z Dulić, Z Marković, J Krizmanić, Dj Milošević, B Miljanović, J Jovanović, D Vidaković, I Živić
Trout farms are one of the major sources of pollution of highland streams and rivers, which are very sensitive ecosystems otherwise scarcely influenced by human activities. A trout farm with low annual production was chosen for comparative monitoring of its effects on the macrozoobenthos, epilithic diatom, and potamoplankton communities in the receiving stream. Research was conducted every 2 months during a period of 1 year (one control point and three localities downstream from the farm). The fish farm discharge induced moderate alteration of environmental factors in the receiving watercourse, viz...
August 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28724105/nitrifying-kinetics-and-the-persistence-of-nitrite-in-the-seine-river-france
#3
M Raimonet, T Cazier, V Rocher, A M Laverman
Although a higher oxidation rate for nitrite than for ammonia generally prevents nitrite accumulation in oxic waters, nitrite concentrations in the Seine River (1-31 μM) exceed European norms. We investigated the kinetics of in situ ammonia- and nitrite-oxidizing communities in river water and wastewater treatment plant (WWTP) effluents to determine the role of pelagic nitrification in the origin and persistence of nitrite downstream of Paris. The main source of nitrite is the major Parisian WWTP, and its persistence, up to tens of kilometers downstream of the plant, is explained by low ammonia and nitrite oxidation rates and high river flow...
May 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28722751/graded-replacement-of-maize-grain-with-molassed-sugar-beet-pulp-modulated-ruminal-microbial-community-and-fermentation-profile-in-vitro
#4
Matthias Münnich, Annabella Khol-Parisini, Fenja Klevenhusen, Barbara Metzler-Zebeli, Qendrim Zebeli
BACKGROUND: Molassed sugar beet pulp (Bp) is a viable alternative to grains in cattle nutrition for reducing human edible energy input. Yet, little is known about the effects of high inclusion rates of Bp on rumen microbiota. This study used an in vitro approach and the qPCR technique to establish the effects of a graded replacement of maize grain (MG) by Bp on the ruminal microbial community, fermentation profile, and nutrient degradation. RESULTS: Six different amounts of Bp (0 to 400 g kg(-1) ) which replaced MG in the diet were tested, using the in vitro semi continuous rumen simulation technique...
July 19, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28718839/dose-dependent-prebiotic-effect-of-lactulose-in-a-computer-controlled-in-vitro-model-of-the-human-large-intestine
#5
Melanie K Bothe, Annet J H Maathuis, Susann Bellmann, Jos M B M van der Vossen, Dirk Berressem, Annalena Koehler, Susann Schwejda-Guettes, Barbara Gaigg, Angelika Kuchinka-Koch, John F Stover
Lactulose, a disaccharide of galactose and fructose, used as a laxative or ammonia-lowering drug and as a functional food ingredient, enhances growth of Bifidobacterium and Lactobacillus at clinically relevant dosages. The prebiotic effect of subclinical dosages of Lactulose, however, remains to be elucidated. This study analyses changes in the microbiota and their metabolites after a 5 days Lactulose treatment using the TIM-2 system, a computer-controlled model of the proximal large intestine representing a complex, high density, metabolically active, anaerobic microbiota of human origin...
July 18, 2017: Nutrients
https://www.readbyqxmd.com/read/28707136/the-formation-of-a-functional-retinal-pigment-epithelium-occurs-on-porous-polytetrafluoroethylene-substrates-independently-of-the-surface-chemistry
#6
Victoria R Kearns, Jack Tasker, Zhuola, Riaz Akhtar, Akash Bachhuka, Krasimir Vasilev, Carl M Sheridan, Rachel L Williams
Subretinal transplantation of functioning retinal pigment epithelial (RPE) cells may have the potential to preserve or restore vision in patients affected by blinding diseases such as age-related macular degeneration (AMD). One of the critical steps in achieving this is the ability to grow a functioning retinal pigment epithelium, which may need a substrate on which to grow and to aid transplantation. Tailoring the physical and chemical properties of the substrate should help the engineered tissue to function in the long term...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28704427/the-influence-of-human-exploration-on-the-microbial-community-structure-and-ammonia-oxidizing-potential-of-the-su-bentu-limestone-cave-in-sardinia-italy
#7
Stefan Leuko, Kaisa Koskinen, Laura Sanna, Ilenia M D'Angeli, Jo De Waele, Paolo Marcia, Christine Moissl-Eichinger, Petra Rettberg
The bacterial diversity in the Su Bentu Cave in Sardinia was investigated by means of 16S rRNA gene-based analysis. This 15 km long cave, carved in Jurassic limestone, hosts a variety of calcite speleothems, and a long succession of subterranean lakes with mixed granite and carbonate sands. The lower level is occasionally flooded by a rising groundwater level, but with only scarce input of organic remains (leaves and charcoal fragments). On the quiet cave pools there are visible calcite rafts, whereas walls are locally coated with manganese deposits...
2017: PloS One
https://www.readbyqxmd.com/read/28681965/amorphizing-of-au-nanoparticles-by-ceox-rgo-hybrid-support-towards-highly-efficient-electrocatalyst-for-n2-reduction-under-ambient-conditions
#8
Si-Jia Li, Di Bao, Miao-Miao Shi, Ba-Ri Wulan, Jun-Min Yan, Qing Jiang
Ammonia synthesis is one of the most kinetically complex and energetically challenging chemical processes in industry and has used the Harber-Bosch catalyst for over a century, which is processed under both harsh pressure (150-350 atm) and hightemperature (623-823 K), wherein the energy and capital intensive Harber-Bosch process has a huge energy cost accounting for about 1%-3% of human's energy consumption. Therefore, there has been a rough and vigorous exploration to find an environmentally benign alternative process...
July 6, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28659525/comparative-evaluation-of-conventional-and-microwave-hydrothermal-carbonization-of-human-biowaste-for-value-recovery
#9
Oluwasola O D Afolabi, M Sohail
This paper compares conventional and microwave hydrothermal carbonization (HTC) of human biowaste (HBW) at 160 °C, 180 °C and 200 °C as a potential technology to recover valuable carbonaceous solid fuel char and organic-rich liquor. Also discussed are the influence of HTC heating methods and temperature on HBW processing conversion into solid fuel char, i.e. yield and post-HTC management, dewaterability rates, particle size distribution and the carbon and energy properties of solid fuel char. While HTC temperatures influenced all parameters investigated, especially yield and properties of end products recovered, heating source effects were noticeable on dewatering rates, char particle sizes and HBW processing/end product recovery rate and, by extension, energy consumed...
June 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28655218/isolation-and-characterization-of-burkholderia-fungorum-gan-35-with-the-outstanding-ammonia-nitrogen-degrading-ability-from-the-tailings-of-rare-earth-element-mines-in-southern-jiangxi-china
#10
Ai-Juan Feng, Xi Xiao, Cong-Cong Ye, Xiao-Ming Xu, Qing Zhu, Jian-Ping Yuan, Yue-Hui Hong, Jiang-Hai Wang
The exploitation of rare-earth-element (REE) mines has resulted in severe ammonia nitrogen pollution and induced hazards to environments and human health. Screening microorganisms with the ammonia nitrogen-degrading ability provides a basis for bioremediation of ammonia nitrogen-polluted environments. In this study, a bacterium with the outstanding ammonia nitrogen-degrading capability was isolated from the tailings of REE mines in southern Jiangxi Province, China. This strain was identified as Burkholderia fungorum Gan-35 according to phenotypic and phylogenetic analyses...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28654800/long-term-investigation-of-constructed-wetland-wastewater-treatment-and-reuse-selection-of-adapted-plant-species-for-metaremediation
#11
Mohamed Mounir Saggaï, Abdelkader Ainouche, Mark Nelson, Florence Cattin, Abdelhak El Amrani
A highly diverse plant community in a constructed wetland was used to investigate an ecological treatment system for human wastewater in an arid climate. The eight-year operation of the system has allowed the identification of a highly adapted and effective plant consortium that is convenient for plant-assisted metaremediation of wastewater. This constructed wetland pilot station demonstrated effective performance over this extended period. Originally, there were twenty-five plant species. However, because of environmental constraints and pressure from interspecific competition, only seven species persisted...
June 24, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28612304/cardiovascular-effects-and-fatality-may-differ-according-to-the-formulation-of-glyphosate-salt-herbicide
#12
Jeong Mi Moon, Byeong Jo Chun, Yong Soo Cho, Sung Do Lee, Young Joon Hong, Min Ho Shin, Eu Jene Jung, Hyun Ho Ryu
This study aimed to compare adverse cardiovascular events and fatalities and to identify the risk factors for fatalities associated with the glyphosate salt herbicide formulation. Additionally, we examined whether glyphosate ammonium salt herbicides increased serum ammonia levels. One hundred forty-seven patients were divided into an ammonium group (glyphosate ammonium salt herbicide) and an isopropylamine (IPA) group (glyphosate IPA salt herbicide) according to the type of glyphosate salt formulation ingested...
June 13, 2017: Cardiovascular Toxicology
https://www.readbyqxmd.com/read/28600910/dried-human-skin-fibroblasts-as-a-new-substratum-for-functional-culture-of-hepatic-cells
#13
Agnieszka Wencel, Karolina Ewa Zakrzewska, Anna Samluk, Bartłomiej Henryk Noszczyk, Dorota Genowefa Pijanowska, Krzysztof Dariusz Pluta
The primary hepatocytes culture is still one of the main challenges in toxicology studies in the drug discovery process, development of in vitro models to study liver function, and cell-based therapies. Isolated hepatocytes display a rapid decline in viability and liver-specific functions including albumin production, conversion of ammonia to urea, and activity of the drug metabolizing enzymes. A number of methods have been developed in order to maintain hepatocytes in their highly differentiated state in vitro...
2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28582755/ultra-sensitive-and-selective-nh3-room-temperature-gas-sensing-induced-by-manganese-doped-titanium-dioxide-nanoparticles
#14
Zamaswazi P Tshabalala, Katekani Shingange, Franscious R Cummings, Odireleng M Ntwaeaborwa, Gugu H Mhlongo, David E Motaung
The study of the fabrication of ultra-high sensitive and selective room temperature ammonia (NH3) and nitrogen dioxide (NO2) gas sensors remains an important scientific challenge in the gas sensing field. This is motivated by their harmful impact on the human health and environment. Therefore, herein, we report for the first time on the gas sensing properties of TiO2 nanoparticles doped with various concentrations of manganese (Mn) (1.0, 1.5, 2.0, 2.5 and 3.0mol.% presented as S1, S2, S3, S4 and S5, respectively), synthesized using hydrothermal method...
May 24, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28569248/effect-of-foliar-application-of-salicylic-acid-hydrogen-peroxide-and-a-xyloglucan-oligosaccharide-on-capsiate-content-and-gene-expression-associatedwith-capsinoids-synthesis-in-capsicum-annuum-l
#15
A Y Zunun-Perez, T Guevara-Figueroa, S N Jimenez-Garcia, A A Feregrino-Perez, F Gautier, R G Guevara-Gonzalez
Capsinoids are non-pungent analogues of capsaicinoids in pepper (Capsicum spp). The absence of pungency, in addition to their biological activities similar to that of capsaicinoids such as anti-inflammatory, antimicrobial, and antioxidant properties, makes capsinoids an excellent option for increasing use in human and animal nutrition, as well as health and pharmaceutical industries. There are only few sources of pepper producing capsinoids, and one of them (accession 509-45-1), Capsicum annuum L., is a potential source for increasing capsinoids content using strategies as controlled elicitation during plant production in the greenhouse...
June 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/28538732/cps1-maintains-pyrimidine-pools-and-dna-synthesis-in-kras-lkb1-mutant-lung-cancer-cells
#16
Jiyeon Kim, Zeping Hu, Ling Cai, Kailong Li, Eunhee Choi, Brandon Faubert, Divya Bezwada, Jaime Rodriguez-Canales, Pamela Villalobos, Yu-Fen Lin, Min Ni, Kenneth E Huffman, Luc Girard, Lauren A Byers, Keziban Unsal-Kacmaz, Christopher G Peña, John V Heymach, Els Wauters, Johan Vansteenkiste, Diego H Castrillon, Benjamin P C Chen, Ignacio Wistuba, Diether Lambrechts, Jian Xu, John D Minna, Ralph J DeBerardinis
Metabolic reprogramming by oncogenic signals promotes cancer initiation and progression. The oncogene KRAS and tumour suppressor STK11, which encodes the kinase LKB1, regulate metabolism and are frequently mutated in non-small-cell lung cancer (NSCLC). Concurrent occurrence of oncogenic KRAS and loss of LKB1 (KL) in cells specifies aggressive oncological behaviour. Here we show that human KL cells and tumours share metabolomic signatures of perturbed nitrogen handling. KL cells express the urea cycle enzyme carbamoyl phosphate synthetase-1 (CPS1), which produces carbamoyl phosphate in the mitochondria from ammonia and bicarbonate, initiating nitrogen disposal...
June 1, 2017: Nature
https://www.readbyqxmd.com/read/28538654/a-monitoring-system-for-laying-hens-that-uses-a-detection-sensor-based-on-infrared-technology-and-image-pattern-recognition
#17
Mauro Zaninelli, Veronica Redaelli, Fabio Luzi, Valentino Bontempo, Vittorio Dell'Orto, Giovanni Savoini
In Italy, organic egg production farms use free-range housing systems with a big outdoor area and a flock of no more than 500 hens. With additional devices and/or farming procedures, the whole flock could be forced to stay in the outdoor area for a limited time of the day. As a consequence, ozone treatments of housing areas could be performed in order to reduce the levels of atmospheric ammonia and bacterial load without risks, due by its toxicity, both for hens and workers. However, an automatic monitoring system, and a sensor able to detect the presence of animals, would be necessary...
May 24, 2017: Sensors
https://www.readbyqxmd.com/read/28531961/challenges-in-photocatalytic-reduction-of-nitrate-as-a-water-treatment-technology
#18
Heather O'Neal Tugaoen, Sergi Garcia-Segura, Kiril Hristovski, Paul Westerhoff
Management of ubiquitous nitrate contamination in drinking water sources is a major engineering challenge due to its negative impacts from eutrophication to immediate risk to human health. Several water treatment technologies exist to manage nitrate pollution in water sources. However, the most widely used technologies are phase separation treatments. In this context, nanoscale photocatalysis emerges as a highly promising transformative technology capable of reducing nitrate to innocuous nitrogen with noticeable selectivity...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28526785/effects-of-arginine-on-growth-virulence-gene-expression-and-stress-tolerance-by-streptococcus-mutans
#19
Brinta Chakraborty, Robert A Burne
Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In contrast, many commensal bacteria produce ammonia through the arginine deiminase system (ADS), which moderates the pH of oral biofilms. Arginine metabolism by the ADS is a significant deterrent to the initiation and progression of dental caries...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28509546/growth-of-nitrosococcus-related-ammonia-oxidizing-bacteria-coincides-with-extremely-low-ph-values-in-wastewater-with-high-ammonia-content
#20
Alexandra Fumasoli, Helmut Bürgmann, David G Weissbrodt, George F Wells, Karin Beck, Joachim Mohn, Eberhard Morgenroth, Kai M Udert
Ammonia oxidation decreases the pH in wastewaters where alkalinity is limited relative to total ammonia. The activity of ammonia oxidizing bacteria (AOB), however, typically decreases with pH and often ceases completely in slightly acidic wastewaters. Nevertheless, nitrification at low pH has been reported in reactors treating human urine, but it has been unclear which organisms are involved. In this study, we followed the population dynamics of ammonia oxidizing organisms and reactor performance in synthetic fully hydrolyzed urine as the pH decreased over time in response to a decrease in the loading rate...
June 5, 2017: Environmental Science & Technology
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