Michael Bidinosti, Paolo Botta, Sebastian Krüttner, Catia C Proenca, Natacha Stoehr, Mario Bernhard, Isabelle Fruh, Matthias Mueller, Debora Bonenfant, Hans Voshol, Walter Carbone, Sarah J Neal, Stephanie M McTighe, Guglielmo Roma, Ricardo E Dolmetsch, Jeffrey A Porter, Pico Caroni, Tewis Bouwmeester, Andreas Lüthi, Ivan Galimberti
SH3 and multiple ankyrin repeat domains 3 (SHANK3) haploinsufficiency is causative for the neurological features of Phelan-McDermid syndrome (PMDS), including a high risk of autism spectrum disorder (ASD). We used unbiased, quantitative proteomics to identify changes in the phosphoproteome of Shank3-deficient neurons. Down-regulation of protein kinase B (PKB/Akt)-mammalian target of rapamycin complex 1 (mTORC1) signaling resulted from enhanced phosphorylation and activation of serine/threonine protein phosphatase 2A (PP2A) regulatory subunit, B56β, due to increased steady-state levels of its kinase, Cdc2-like kinase 2 (CLK2)...
March 11, 2016: Science