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https://www.readbyqxmd.com/read/28432341/detection-of-red-blood-cell-surface-antigens-by-probe-triggered-cell-collision-and-flow-retardation-in-an-autonomous-microfluidic-system
#1
Éva Sautner, Krisztián Papp, Eszter Holczer, Eszter L Tóth, Rita Ungai-Salánki, Bálint Szabó, Péter Fürjes, József Prechl
Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteristics of blood flow in autonomous microfluidic devices with the aim to realize sensitive detection of interactions between particulate elements of the blood and the appropriately modified surfaces of the system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28430889/accuracy-of-diagnostic-tests-for-schistosoma-mansoni-infection-in-asymptomatic-eritrean-refugees-serology-and-poc-cca-against-stool-microscopy
#2
Afona Chernet, Kerstin Kling, Véronique Sydow, Esther Kuenzli, Christoph Hatz, Jürg Utzinger, Lisette van Lieshout, Hanspeter Marti, Niklaus D Labhardt, Andreas Neumayr
Background: The unprecedented increase in number of African refugees arriving in Europe is confronting clinicians and general practitioners with the question of whether or not and how to screen migrants from endemic regions for Schistosoma mansoni infection. Methods: We assessed the accuracy of three different diagnostic tests for S.mansoni infection (stool microscopy [samples prepared by sedimentation technique], serology, and point-of-care circulating cathodic antigen [POC-CCA] urine cassette test) in 107 newly arrived asymptomatic Eritrean refugees in Switzerland...
April 19, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28430713/a-national-survey-of-neonatologists-barriers-and-prerequisites-to-introduce-point-of-care-ultrasound-in-neonatal-icus
#3
Hussnain S Mirza, Gregory Logsdon, Anoop Pulickal, Mark Stephens, Rajan Wadhawan
Point-of-care (POC) ultrasound refers to the use of portable imaging. Although POC ultrasound is widely available to the neonatologists in Australia and Europe, neonatologists in the United States report limited availability. Our objective was to seek the US neonatologists' perception of barriers and prerequisites in adopting POC ultrasound in neonatal intensive care units. An online survey link was sent via e-mail to 3000 neonatologists included in the database maintained by the American Academy of Pediatrics...
April 20, 2017: Ultrasound Quarterly
https://www.readbyqxmd.com/read/28430186/independence-of-nutrient-limitation-and-carbon-dioxide-impacts-on-the-southern-ocean-coccolithophore-emiliania-huxleyi
#4
Marius N Müller, Thomas W Trull, Gustaaf M Hallegraeff
Future oceanic conditions induced by anthropogenic greenhouse gas emissions include warming, acidification and reduced nutrient supply due to increased stratification. Some parts of the Southern Ocean are expected to show rapid changes, especially for carbonate mineral saturation. Here we compare the physiological response of the model coccolithophore Emiliania huxleyi (strain EHSO 5.14, originating from 50(o)S, 149(o)E) with pH/CO2 gradients (mimicking ocean acidification ranging from 1 to 4 × current pCO2 levels) under nutrient-limited (nitrogen and phosphorus) and -replete conditions...
April 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28425520/an-innovative-chemical-strategy-for-pcr-free-genetic-detection-of-pathogens-by-an-integrated-electrochemical-biosensor
#5
S Petralia, E L Sciuto, M L Di Pietro, M Zimbone, M G Grimaldi, S Conoci
An innovative chemical strategy integrated in a miniaturized electrochemical device was developed for sensitive detection of a pathogen genome (HBV virus) without any amplification step. The results show a limit of detection comparable to the standard qRT-PCR method (20 copies per reaction), paving the way to future development of genetic PoC devices addressing automatized and low-cost molecular diagnostics.
April 20, 2017: Analyst
https://www.readbyqxmd.com/read/28422968/the-impact-of-a-point-of-care-testing-device-on-cvd-risk-assessment-completion-in-new-zealand-primary-care-practice-a-cluster-randomised-controlled-trial-and-qualitative-investigation
#6
Sue Wells, Natasha Rafter, Timothy Kenealy, Geoff Herd, Kyle Eggleton, Rose Lightfoot, Kim Arcus, Angela Wadham, Yannan Jiang, Chris Bullen
OBJECTIVES: To assess the effect of a point of care (POC) device for testing lipids and HbA1c in addition to testing by community laboratory facilities (usual practice) on the completion of cardiovascular disease (CVD) risk assessments in general practice. METHODS: We conducted a pragmatic, cluster randomised controlled trial in 20 New Zealand general practices stratified by size and rurality and randomised to POC device plus usual practice or usual practice alone (controls)...
2017: PloS One
https://www.readbyqxmd.com/read/28420603/the-coags-uncomplicated-app-fulfilling-educational-gaps-around-diagnosis-and-laboratory-testing-of-coagulation-disorders
#7
Craig Kessler, Ellinor I Peerschke, Meera B Chitlur, Roshni Kulkarni, Natalia Holot, David L Cooper
BACKGROUND: Patients with coagulation disorders may present to a variety of physician specialties; however, accurate and efficient diagnosis can be challenging for physicians not specialized in hematology, due to identified gaps in knowledge around appropriate laboratory assays and interpretation of test results. Coags Uncomplicated was developed to fill this unmet educational need by increasing practical knowledge of coagulation disorders among nonexpert physicians and other health care professionals (HCPs) in a point-of-care (POC) setting...
April 18, 2017: JMIR Medical Education
https://www.readbyqxmd.com/read/28420103/electrochemical-genosensing-of-circulating-biomarkers
#8
REVIEW
Susana Campuzano, Paloma Yáñez-Sedeño, José Manuel Pingarrón
Management and prognosis of diseases requires the measurement in non- or minimally invasively collected samples of specific circulating biomarkers, consisting of any measurable or observable factors in patients that indicate normal or disease-related biological processes or responses to therapy. Therefore, on-site, fast and accurate determination of these low abundance circulating biomarkers in scarcely treated body fluids is of great interest for health monitoring and biological applications. In this field, electrochemical DNA sensors (or genosensors) have demonstrated to be interesting alternatives to more complex conventional strategies...
April 14, 2017: Sensors
https://www.readbyqxmd.com/read/28415168/an-update-on-endoscopic-management-of-post-liver-transplant-biliary-complications
#9
Hyun Woo Lee, Najmul Hassan Shah, Sung Koo Lee
Biliary complications are the most common post-liver transplant (LT) complications with an incidence of 15%-45%. Furthermore, such complications are reported more frequently in patients who undergo a living-donor LT (LDLT) compared to a deceased-donor LT (DDLT). Most post-LT biliary complications involve biliary strictures, bile leakage, and biliary stones, although many rarer events, such as hemobilia and foreign bodies, contribute to a long list of related conditions. Endoscopic treatment of post-LT biliary complications has evolved rapidly, with new and effective tools improving both outcomes and success rates; in fact, the latter now consistently reach up to 80%...
April 17, 2017: Clinical Endoscopy
https://www.readbyqxmd.com/read/28414454/novel-soil-profile-sensing-to-monitor-organic-c-stocks-and-condition
#10
Raphael A Viscarra Rossel, Craig R Lobsey, Chris Sharman, Paul Flick, Gordon McLachlan
Soil information is needed for environmental monitoring to address current concerns over food, water and energy securities, land degradation and climate change. We developed the {\sc Soil Condition ANalyses System (SCANS)} to help address these needs. It integrates an automated soil core sensing system (CSS) with statistical analytics and modelling to enable the characterisation of soil at fine depth resolutions and across landscapes. The CSS's sensors include a gamma-ray densitometer to measure bulk density, digital cameras to image the measured soil and a visible--near infrared (vis--NIR) spectrometer to measure iron oxides and clay mineralogy...
April 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28411630/an-immunochromatographic-assay-for-carcinoembryonic-antigen-on-cotton-thread-using-a-composite-of-carbon-nanotubes-and-gold-nanoparticles-as-reporters
#11
Xiaobo Jia, Tingting Song, Yan Liu, Lili Meng, Xun Mao
This paper describes a low-cost, sensitive, visual and rapid immunochromatographic assay method on cotton thread for carcinoembryonic antigen (CEA) detection by using novel carbon nanotube/gold nanoparticles (CNT/GNPs) nanocomposite reporter probe. CEA, a lung cancer protein biomarker, was used as analyte to demonstrate the principle of the immunochromatographic assay on cotton thread biosensor. In the presence of target CEA, the decreasing aggregation amount of CNT/GNPs nanocomposite reporter probes on the test zone induced directly readout by naked eye...
May 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28410701/lamp-an-innovative-poc-tool-for-diagnosing-pulmonary-tb-in-remote-areas
#12
Steffi Becker
No abstract text is available yet for this article.
April 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28405258/rapid-isolation-of-blood-plasma-using-a-cascaded-inertial-microfluidic-device
#13
M Robinson, H Marks, T Hinsdale, K Maitland, G Coté
Blood, saliva, mucus, sweat, sputum, and other biological fluids are often hindered in their ability to be used in point-of-care (POC) diagnostics because their assays require some form of off-site sample pre-preparation to effectively separate biomarkers from larger components such as cells. The rapid isolation, identification, and quantification of proteins and other small molecules circulating in the blood plasma from larger interfering molecules are therefore particularly important factors for optical blood diagnostic tests, in particular, when using optical approaches that incur spectroscopic interference from hemoglobin-rich red blood cells (RBCs)...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28400120/impact-of-point-of-care-pre-procedure-creatinine-and-egfr-testing-in-patients-with-st-segment-elevation-myocardial-infarction-undergoing-primary-pci-the-pilot-statcreat-study
#14
Grigoris V Karamasis, James Hampton-Till, Firas Al-Janabi, Shah Mohdnazri, Michael Parker, Adam Ioannou, Rohan Jagathesan, Alagmir Kabir, Jeremy W Sayer, Nicholas M Robinson, Rajesh K Aggarwal, Gerald J Clesham, Reto A Gamma, Paul A Kelly, Kare H Tang, John R Davies, Thomas Keeble
BACKGROUND: Contrast-induced acute kidney injury (CI-AKI) is a recognised complication during primary PCI that affects short and long term prognosis. The aim of this study was to assess the impact of point-of-care (POC) pre-PPCI creatinine and eGFR testing in STEMI patients. METHODS: 160 STEMI patients (STATCREAT group) with pre-procedure POC testing of Cr and eGFR were compared with 294 consecutive retrospective STEMI patients (control group). Patients were further divided into subjects with or without pre-existing CKD...
April 5, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28399329/adapting-a-large-database-of-point-of-care-summarized-guidelines-a-process-description
#15
Nicolas Delvaux, Stijn Van de Velde, Bert Aertgeerts, Martine Goossens, Benjamin Fauquert, Ilka Kunnamo, Paul Van Royen
RATIONALE, AIMS AND OBJECTIVES: Questions posed at the point of care (POC) can be answered using POC summarized guidelines. To implement a national POC information resource, we subscribed to a large database of POC summarized guidelines to complement locally available guidelines. Our challenge was in developing a sustainable strategy for adapting almost 1000 summarized guidelines. The aim of this paper was to describe our process for adapting a database of POC summarized guidelines. METHODS: An adaptation process based on the ADAPTE framework was tailored to be used by a heterogeneous group of participants...
February 2017: Journal of Evaluation in Clinical Practice
https://www.readbyqxmd.com/read/28397478/point-of-care-cardiac-troponin-assay-analytical-performances-for-their-use-in-clinical-routine
#16
Anne M Dupuy, Solenne Baillet, Richard Dumont, Isabelle Giraud, Stephanie Badiou, Anne S Bargnoux, Nils Kuster, Francois Roubille, Jean Paul Cristol
BACKGROUND: We report the analytical and clinical performances of the Alere Triage Cardiac3© Panel on the Triage MeterPro© instrument, comparing concordance with hs-cTnT results from central laboratory above the respective 99th percentiles and determining the clinical sensitivity within the framework of AMI. METHODS: The concordance was obtained with these two methods among unselected patients admitted to both the emergency and cardiology departments. RESULTS: The LoD of the assay is 0...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397461/analytical-and-clinical-validation-of-a-point-of-care-cardiac-troponin-t-test-with-an-improved-detection-limit
#17
Carsten Jungbauer, Julian Hupf, Evangelos Giannitsis, Johann Frick, Anna Slagman, Christoph Ehret, Nicolas Herbert, Christine Jung, Rainer Zerback, Thomas Bertsch, Michael Christ
BACKGROUND: The point-of-care test Roche CARDIAC POC Troponin T (PoC TnT) is an improved assay which has been developed for the Roche cobas h 232 system. METHODS: We performed a multicentre evaluation (four sites) to assess the analytical performance of the PoC TnT assay and to compare it with the central laboratory Elecsys® troponin T high sensitive (lab cTnT-hs) assay. RESULTS: The relative mean differences found in method comparisons of PoC TnT vs...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28393783/coagulopathies-in-cyanotic-cardiac-patients-an-analysis-with-three-point-of-care-testing-devices-thromboelastography-rotational-thromboelastometry-and-sonoclot-analyzer
#18
Vandana Bhardwaj, Poonam Malhotra, Suruchi Hasija, Ujjwal Kumar Chowdury, Neha Pangasa
INTRODUCTION: In the last few years, viscoelastic point-of-care (POC) coagulation devices such as thromboelastography (TEG), rotational thromboelastometry (ROTEM), and Sonoclot (SON) analyzer have been increasingly used in major surgeries for timely assessment and management of coagulopathies. The aim of the present study was to evaluate coagulation profile of cyanotic cardiac patients with TEG, ROTEM, and SON analyzer. In addition, we assessed the correlation of standard laboratory coagulation tests and postoperative chest drain output (CDO) with the parameters of POC testing devices...
April 2017: Annals of Cardiac Anaesthesia
https://www.readbyqxmd.com/read/28392729/essential-aspects-of-external-quality-assurance-for-point-of-care-testing
#19
REVIEW
Anne Stavelin, Sverre Sandberg
External quality assurance (EQA) or proficiency testing for point-of-care (POC) testing is in principle similar to EQA for larger hospital laboratories, but the participants are different. The participants are usually health care personnel with little or no knowledge of laboratory medicine. The implication of this is that the EQA provider has to a) convince the participants that participation in EQA schemes are important, b) be able to circulate materials with reasonable time intervals, c) produce feedback reports that are understandable, and d) offer help and guidance to the participants when needed...
February 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28392237/routine-molecular-point-of-care-testing-for-respiratory-viruses-in-adults-presenting-to-hospital-with-acute-respiratory-illness-respoc-a-pragmatic-open-label-randomised-controlled-trial
#20
Nathan J Brendish, Ahalya K Malachira, Lawrence Armstrong, Rebecca Houghton, Sandra Aitken, Esther Nyimbili, Sean Ewings, Patrick J Lillie, Tristan W Clark
BACKGROUND: Respiratory virus infection is a common cause of hospitalisation in adults. Rapid point-of-care testing (POCT) for respiratory viruses might improve clinical care by reducing unnecessary antibiotic use, shortening length of hospital stay, improving influenza detection and treatment, and rationalising isolation facility use; however, insufficient evidence exists to support its use over standard clinical care. We aimed to assess the effect of routine POCT on a broad range of clinical outcomes including antibiotic use...
April 6, 2017: Lancet Respiratory Medicine
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