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https://www.readbyqxmd.com/read/28087761/proteomics-technologies-and-their-applications
#1
REVIEW
Bilal Aslam, Madiha Basit, Muhammad Atif Nisar, Mohsin Khurshid, Muhammad Hidayat Rasool
Proteomics involves the applications of technologies for the identification and quantification of overall proteins present content of a cell, tissue or an organism. It supplements the other "omics" technologies such as genomic and transcriptomics to expound the identity of proteins of an organism, and to cognize the structure and functions of a particular protein. Proteomics-based technologies are utilized in various capacities for different research settings such as detection of various diagnostic markers, candidates for vaccine production, understanding pathogenicity mechanisms, alteration of expression patterns in response to different signals and interpretation of functional protein pathways in different diseases...
February 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28086947/quantifying-growing-versus-non-growing-ovarian-follicles-in-the-mouse
#2
Bahar Uslu, Carola Conca Dioguardi, Monique Haynes, De-Qiang Miao, Meltem Kurus, Gloria Hoffman, Joshua Johnson
BACKGROUND: A standard histomorphometric approach has been used for nearly 40 years that identifies atretic (e.g., dying) follicles by counting the number of pyknotic granulosa cells (GC) in the largest follicle cross-section. This method holds that if one pyknotic granulosa nucleus is seen in the largest cross section of a primary follicle, or three pyknotic cells are found in a larger follicle, it should be categorized as atretic. Many studies have used these criteria to estimate the fraction of atretic follicles that result from genetic manipulation or environmental insult...
January 13, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28086870/a-pilot-study-of-a-community-health-agent-led-type-2-diabetes-self-management-program-using-motivational-interviewing-based-approaches-in-a-public-primary-care-center-in-s%C3%A3-o-paulo-brazil
#3
Thais Moura Ribeiro do Valle Nascimento, Ken Resnicow, Marcia Nery, Alexandra Brentani, Elizabeth Kaselitz, Pooja Agrawal, Simanjit Mand, Michele Heisler
BACKGROUND: Rates of noncommunicable diseases (NCDs) such as type 2 diabetes are escalating in low and middle-income countries such as Brazil. Scalable primary care-based interventions are needed to improve self-management and clinical outcomes of adults with diabetes. This pilot study examines the feasibility, acceptability, and outcomes of training community health agents (CHAs) in Motivational Interviewing (MI)-based counseling for patients with poorly controlled diabetes in a primary care center in São Paulo, Brazil...
January 13, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28080083/the-empathy-impulse-a-multinomial-model-of-intentional-and-unintentional-empathy-for-pain
#4
C Daryl Cameron, Victoria L Spring, Andrew R Todd
Empathy for pain is often described as automatic. Here, we used implicit measurement and multinomial modeling to formally quantify unintentional empathy for pain: empathy that occurs despite intentions to the contrary. We developed the pain identification task (PIT), a sequential priming task wherein participants judge the painfulness of target experiences while trying to avoid the influence of prime experiences. Using multinomial modeling, we distinguished 3 component processes underlying PIT performance: empathy toward target stimuli (Intentional Empathy), empathy toward prime stimuli (Unintentional Empathy), and bias to judge target stimuli as painful (Response Bias)...
January 12, 2017: Emotion
https://www.readbyqxmd.com/read/28079970/categorizing-the-magnitude-and-frequency-of-exposure-to-uncivil-behaviors-a-new-approach-for-more-meaningful-interventions
#5
Dena Evans
PURPOSE: To examine turnover intentions, as well as the prevalence and frequency of uncivil behaviors, from the perspective of registered nurses, respiratory therapists, and imaging professionals, using a new method to categorize exposure magnitude. DESIGN AND METHODS: Data were collected using the 22-item Negative Acts Questionnaire-Revised (NAQ-R). Additional items, informed by Price and Mueller's causal model of turnover, were included, as were select demographic variables...
January 12, 2017: Journal of Nursing Scholarship
https://www.readbyqxmd.com/read/28079351/effect-of-age-on-outcomes-and-practice-patterns-for-patients-with-anterior-communicating-artery-aneurysms
#6
Federico Cagnazzo, Waleed Brinjikji, Giuseppe Lanzino
BACKGROUND: With widespread utilization of noninvasive imaging, small enraptured anterior communicating artery (AcoA) aneurysms are increasingly detected in the general population, particularly in elderly patients. We investigated the association between age and clinical characteristics and outcomes of patients with unruptured AcoA aneurysms. METHODS: Between January 2008 and May 2016, information on 179 consecutive patients with unruptured AcoA aneurysms was obtained and included demographic data, aneurysm features, risk factors for formation and rupture, treatment type, complications, and follow-up information...
January 12, 2017: Journal of Neurosurgical Sciences
https://www.readbyqxmd.com/read/28078571/analyzing-spatial-data-from-mouse-tracker-methodology-an-entropic-approach
#7
Antonio Calcagnì, Luigi Lombardi, Simone Sulpizio
Mouse tracker methodology has recently been advocated to explore the motor components of the cognitive dynamics involved in experimental tasks like categorization, decision-making, and language comprehension. This methodology relies on the analysis of computer-mouse trajectories, by evaluating whether they significantly differ in terms of direction, amplitude, and location when a given experimental factor is manipulated. In this kind of study, a descriptive geometric approach is usually adopted in the analysis of raw trajectories, where they are summarized with several measures, such as maximum-deviation and area under the curve...
January 11, 2017: Behavior Research Methods
https://www.readbyqxmd.com/read/28077070/scopa-and-meta-scopa-software-for-the-analysis-and-aggregation-of-genome-wide-association-studies-of-multiple-correlated-phenotypes
#8
Reedik Mägi, Yury V Suleimanov, Geraldine M Clarke, Marika Kaakinen, Krista Fischer, Inga Prokopenko, Andrew P Morris
BACKGROUND: Genome-wide association studies (GWAS) of single nucleotide polymorphisms (SNPs) have been successful in identifying loci contributing genetic effects to a wide range of complex human diseases and quantitative traits. The traditional approach to GWAS analysis is to consider each phenotype separately, despite the fact that many diseases and quantitative traits are correlated with each other, and often measured in the same sample of individuals. Multivariate analyses of correlated phenotypes have been demonstrated, by simulation, to increase power to detect association with SNPs, and thus may enable improved detection of novel loci contributing to diseases and quantitative traits...
January 11, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28075696/dual-eligibles-experience-of-care-with-north-carolina-s-patient-centered-medical-home
#9
Sarah Grantham, Debora Goetz Goldberg, Donna Lind Infeld
Although individuals enrolled in both Medicare and Medicaid (dual eligibles) are among those with the nation's greatest need, at $300 billion per year, their care is also expensive and beset by quality problems. Previous research found problems associated with inadequate coordination of benefits and services; however, these studies have largely used quantitative approaches and focused on providers-few studies have explored the perspective of dual eligible patients. In an effort to improve care and reduce costs, North Carolina (NC) developed a Patient-Centered Medical Home (PCMH) model centered on a continuous relationship with a primary care provider who is responsible for coordination of services and addressing patients' health care needs by providing direct services or arranging care with other qualified professionals...
January 11, 2017: Population Health Management
https://www.readbyqxmd.com/read/28074598/prediction-of-skin-sensitization-potency-using-machine-learning-approaches
#10
Qingda Zang, Michael Paris, David M Lehmann, Shannon Bell, Nicole Kleinstreuer, David Allen, Joanna Matheson, Abigail Jacobs, Warren Casey, Judy Strickland
The replacement of animal use in testing for regulatory classification of skin sensitizers is a priority for US federal agencies that use data from such testing. Machine learning models that classify substances as sensitizers or non-sensitizers without using animal data have been developed and evaluated. Because some regulatory agencies require that sensitizers be further classified into potency categories, we developed statistical models to predict skin sensitization potency for murine local lymph node assay (LLNA) and human outcomes...
January 10, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28071246/family-perspectives-on-organ-and-tissue-donation-for-transplantation-a-principlist-analysis
#11
Marcelo José Dos Santos, Lydia Feito
BACKGROUND: The family interview context is permeated by numerous ethical issues which may generate conflicts and impact on organ donation process. OBJECTIVE: This study aims to analyze the family interview process with a focus on principlist bioethics. METHOD: This exploratory, descriptive study uses a qualitative approach. The speeches were collected using the following prompt: "Talk about the family interview for the donation of organs and tissues for transplantation, from the preparation for the interview to the decision of the family to donate or not...
January 1, 2017: Nursing Ethics
https://www.readbyqxmd.com/read/28069667/from-loquacious-to-reticent-understanding-patient-health-information-communication-to-guide-consumer-health-it-design
#12
Rupa S Valdez, Thomas M Guterbock, Kara Fitzgibbon, Ishan C Williams, Claire A Wellbeloved-Stone, Jaime E Bears, Hannah K Menefee
BACKGROUND AND SIGNIFICANCE: It is increasingly recognized that some patients self-manage in the context of social networks rather than alone. Consumer health information technology (IT) designed to support socially embedded self-management must be responsive to patients' everyday communication practices. There is an opportunity to improve consumer health IT design by explicating how patients currently leverage social media to support health information communication. OBJECTIVE: The objective of this study was to determine types of health information communication patterns that typify Facebook users with chronic health conditions to guide consumer health IT design...
January 9, 2017: Journal of the American Medical Informatics Association: JAMIA
https://www.readbyqxmd.com/read/28068344/an-empirical-analysis-of-rough-set-categorical-clustering-techniques
#13
Jamal Uddin, Rozaida Ghazali, Mustafa Mat Deris
Clustering a set of objects into homogeneous groups is a fundamental operation in data mining. Recently, many attentions have been put on categorical data clustering, where data objects are made up of non-numerical attributes. For categorical data clustering the rough set based approaches such as Maximum Dependency Attribute (MDA) and Maximum Significance Attribute (MSA) has outperformed their predecessor approaches like Bi-Clustering (BC), Total Roughness (TR) and Min-Min Roughness(MMR). This paper presents the limitations and issues of MDA and MSA techniques on special type of data sets where both techniques fails to select or faces difficulty in selecting their best clustering attribute...
2017: PloS One
https://www.readbyqxmd.com/read/28067547/barriers-and-enablers-to-modifying-sleep-behavior-in-adolescents-and-young-adults-a-qualitative-investigation
#14
Jessica L Paterson, Amy C Reynolds, Mitch Duncan, Corneel Vandelanotte, Sally A Ferguson
OBJECTIVE: Many young adults obtain less than the recommended sleep duration for healthy and safe functioning. Behavior change interventions have had only moderate success in increasing sleep duration for this cohort. This may be because the way young adults think about sleep, including their willingness and ability to change sleep behavior, is unknown. The purpose of the present study was to determine what changes, if any, young adults are willing to make to their sleep behavior, and to identify factors that may enable or prevent these changes...
January 9, 2017: Behavioral Sleep Medicine
https://www.readbyqxmd.com/read/28066099/genistein-synergizes-centchroman-action-in-human-breast-cancer-cells
#15
Shweta Kaushik, Hari Shyam, Ramesh Sharma, Anil K Balapure
OBJECTIVES: Despite the progress in the diagnosis and treatment of breast cancer, it remains a major health problem in women. Natural flavones along with chemotherapeutic agents enhance therapeutic response and minimize toxicity of chemical agents. Centchroman (CC) colloquially called as ormeloxifene, is a nonsteroidal oral contraceptive categorized as selective estrogen receptor modulator with anti-breast cancer activity. Genistein (GN), an isoflavone found mainly in soy products possesses anti-cancerous potential against a number of cancers including breast...
November 2016: Indian Journal of Pharmacology
https://www.readbyqxmd.com/read/28064041/recovering-fine-details-from-under-resolved-electron-tomography-data-using-higher-order-total-variation-%C3%A2-1-regularization
#16
Toby Sanders, Anne Gelb, Rodrigo B Platte, Ilke Arslan, Kai Landskron
Over the last decade or so, reconstruction methods using ℓ1 regularization, often categorized as compressed sensing (CS) algorithms, have significantly improved the capabilities of high fidelity imaging in electron tomography. The most popular ℓ1 regularization approach within electron tomography has been total variation (TV) regularization. In addition to reducing unwanted noise, TV regularization encourages a piecewise constant solution with sparse boundary regions. In this paper we propose an alternative ℓ1 regularization approach for electron tomography based on higher order total variation (HOTV)...
January 3, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28063260/prevalence-of-communication-between-mothers-and-daughters-on-sexuality-and-associated-factors-in-sagamu-southwest-nigeria
#17
Oluwadamilola A Opawale, Victor J Animasahun, Helena J Chapman
BACKGROUND: As adolescents transition from childhood to adulthood, effective mother-daughter communication on sexuality plays a role in reducing high-risk behaviours linked to human immunodeficiency virus (HIV) infection and early pregnancy and childbirth. The objective of the study was to examine the prevalence of mother-daughter communication on sexuality and associated factors in Sagamu, southwest Nigeria. METHODS: Using multistage sampling, surveys were conducted using 234 female youth enrolled at a secondary school...
January 7, 2017: International Journal of Adolescent Medicine and Health
https://www.readbyqxmd.com/read/28061021/comprehensive-assessment-of-tumour-budding-on-cytokeratin-stains-in-colorectal-cancer
#18
Gregor Rieger, Viktor H Koelzer, Heather E Dawson, Martin D Berger, Marion Hädrich, Daniel Inderbitzin, Alessandro Lugli, Inti Zlobec
BACKGROUND: Tumour budding in colorectal cancer (CRC) is a recognized prognostic parameter. Aim of this study is to address the use of cytokeratin immunostaining for visualization and scoring of tumour buds. METHODS: Ten hotspots (0.238 mm(2) ) of peritumoural (PTB) and intratumoural (ITB) budding were evaluated in surgical resections from 215 patients. The budding counts in the 10 densest regions anywhere in the tumour were combined into an overall tumour budding (OTB) score...
January 6, 2017: Histopathology
https://www.readbyqxmd.com/read/28060568/inspection-frequency-sociodemographic-factors-and-food-safety-violations-in-chain-and-nonchain-restaurants-philadelphia-pennsylvania-2013-2014
#19
Sarah E Leinwand, Karen Glanz, Brendan T Keenan, Charles C Branas
OBJECTIVES: We explored how restaurant inspection frequency and restaurant neighborhood sociodemographic characteristics are related to food safety inspection outcomes in chain and nonchain restaurants to better understand external factors that may influence inspection outcomes. METHODS: We categorized the results of restaurant inspections in Philadelphia, Pennsylvania, in 2013 and 2014 by restaurant type (chain or nonchain), inspection frequency (1, 2, or ≥3 per 2-year study period), and violation type (total number of violations, foodborne-illness risk factor violation, or good retail practice violation)...
January 1, 2017: Public Health Reports
https://www.readbyqxmd.com/read/28057565/development-and-validation-of-a-continuously-age-adjusted-measure-of-patient-condition-for-hospitalized-children-using-the-electronic-medical-record
#20
Michael J Rothman, Joseph J Tepas, Andrew J Nowalk, James E Levin, Joan M Rimar, Albert Marchetti, Allen L Hsiao
Awareness of a patient's clinical status during hospitalization is a primary responsibility for hospital providers. One tool to assess status is the Rothman Index (RI), a validated measure of patient condition for adults, based on empirically derived relationships between 1-year post-discharge mortality and each of 26 clinical measurements available in the electronic medical record. However, such an approach cannot be used for pediatrics, where the relationships between risk and clinical variables are distinct functions of patient age, and sufficient 1-year mortality data for each age group simply does not exist...
January 2, 2017: Journal of Biomedical Informatics
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