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cordycepic acid

Tianqiao Yong, Minglong Zhang, Diling Chen, Ou Shuai, Shaodan Chen, Jiyan Su, Chunwei Jiao, Delong Feng, Yizhen Xie
ETHNOPHARMACOLOGICAL RELEVANCE: Cordyceps militaris was recorded in the classic traditional Chinese medicine book with the main functions of "protecting liver and enhancing kidney functions", influencing serum uric acid levels. AIM OF STUDY: The aim is to investigate the hypouricemic effects and possible mechanism of C. militaris in hyperuricemic mice. MATERIALS AND METHODS: A water extract (WECM) was prepared by decocting C. militaris directly at 80 (o)C in water bath, followed by lyophilization...
October 4, 2016: Journal of Ethnopharmacology
Xiaolan Liu, Narasimha-Kumar Kopparapu, Yao Li, Yongping Deng, Xiqun Zheng
A fibrinolytic enzyme was produced by the medicinal mushroom, Cordyceps militaris using submerged fermentation. The enzyme was purified from culture supernatant by hydrophobic interaction, ion exchange and gel filtration chromatographies. It was purified by 36 fold, with a specific activity of 1,467.4U/mg protein and the final yield was 5.8%. The molecular weight of the enzyme as determined by SDS-PAGE and gel filtration was 28kDa and 24.5kDa, respectively, and its isoelectric point (pI) was 9.0±0.2. It was found to be a glycoprotein with carbohydrate content of 1...
September 15, 2016: International Journal of Biological Macromolecules
Jian-Hui Xiao, Yao Zhang, Gui-You Liang, Ru-Ming Liu, Xiao-Gang Li, Ling-Tao Zhang, Dai-Xiong Chen, Jian-Jiang Zhong
The antibacterial agent helvolic acid, which was isolated from the active antitumor fraction of Cordyceps taii, showed potent cytotoxicity against different human cancer cells. In the present study, the in vivo antitumor effect of helvolic acid was investigated in murine sarcoma S180 tumor-bearing mice. Doses of 10 and 20 mg/kg/day helvolic acid did not exert significant antitumor activity. Interestingly, co-administration of 10 mg/kg/day helvolic acid and 20 mg/kg/day cyclophosphamide (CTX) - a well-known chemotherapy drug - showed promising antitumor activity with a growth inhibitory rate of 70...
September 6, 2016: Experimental Biology and Medicine
Zhen-Yuan Zhu, Ming-Zhu Guo, Fei Liu, You Luo, Lu Chen, Meng Meng, Xiao-Ting Wang, Yong-Min Zhang
The structural properties and the inhibition on α-d-glucosidase activity of the low molecular weight (LCMPs-II) obtained from the optimized acid hydrolysis of the Cordyceps militaris polysaccharides (CMPs) were investigated in this paper. The LCMPs-II with a molecular weight of 28 KDa mainly composed of rhamnose, xylose and glucose with the molar ratio of 1: 2.19: 6.73 was separated from LCMPs-I which was the acid hydrolysis product of CMPs by chromatography on Sephadex G-100 column. The solubility of LCMPs-II was tested to be 32...
August 23, 2016: International Journal of Biological Macromolecules
Wonse Suh, Gyeongsug Nam, Woo Seok Yang, Gi-Ho Sung, Sang Hee Shim, Jae Youl Cho
Cordyceps bassiana is one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions...
August 19, 2016: Biomolecules & Therapeutics
Xiao-gang Li, Wei-dong Pan, Xiao-jie Zhang, Jian-hui Xiao
OBJECTIVE: To seperate and identify the chemicals from the antitumor fraction of Cordyceps taii mycelia powder. METHODS: The mycelia of Cordyceps taii were prepared by the submerged fermentation technique. Chemical entities in the antitumor fraction of Cordyceps taii were isolated and purified by using different column chromatographies (silica gel, Sephadex LH-20 and MCI), and semi-preparative HPLC method. Theirs chemical structures were then identified by different spectrum techniques such as EI, ESI and 1D/2D-NMR, etc...
October 2015: Zhong Yao Cai, Zhongyaocai, Journal of Chinese Medicinal Materials
Shashidhar M Ghatnur, Giridhar Parvatam, Manohar Balaraman
BACKGROUND: Cordyceps sinensis (CS) is a traditional Chinese medicine contains potent active metabolites such as nucleosides and polysaccharides. The submerged cultivation technique is studied for the large scale production of CS for biomass and metabolites production. OBJECTIVE: To optimize culture conditions for large-scale production of CS1197 biomass and metabolites production. MATERIALS AND METHODS: The CS1197 strain of CS was isolated from dead larvae of natural CS and the authenticity was assured by the presence of two major markers adenosine and cordycepin by high performance liquid chromatography and mass spectrometry...
October 2015: Pharmacognosy Magazine
Shan Lin, Zhi-Qiang Liu, Ya-Ping Xue, Peter James Baker, Hui Wu, Feng Xu, Yi Teng, Mgavi Elombe Brathwaite, Yu-Guo Zheng
Hirsutella sinensis is considered as the only correct anamorph of Ophiocordyceps sinensis. To improve cordycepin and cordycepic acid production in H. sinensis, the biosynthetic pathways of cordycepin and cordycepic acid were predicted, and verified by cloning and expressing genes involved in these pathways, respectively. Then, 5'-nucleotidase participating in biosynthetic pathway of cordycepin, hexokinase, and glucose phosphate isomerase involved in biosynthetic pathway of cordycepic acid, were demonstrated playing important roles in the corresponding biosynthetic pathway by real-time PCR, accompanying with significantly up-regulated 15...
June 2016: Applied Biochemistry and Biotechnology
Ting Hu, Chenbo Jiang, Qilin Huang, Fengyuan Sun
An exopolysaccharide (EPS) was fractionated from fermentation media of a Cordyceps sinensis fungus (Cs-HK1) by ethanol precipitation at 2/5 volume ratio of ethanol/media. Its structural characteristics were elucidated by FT-IR, GC, GC-MS, 1D and 2D NMR combined with periodate oxidation, Smith degradation, partial acid hydrolysis, and methylation analysis. Furthermore, the immunomodulatory activity of EPS was evaluated by the model of cyclophosphamide-induced immunosuppression. The results from monosaccharide composition and partial acid hydrolysis indicated that EPS almost consisted of glucose excluding a trace amount of mannose...
May 20, 2016: Carbohydrate Polymers
Zhi-Bo Chu, Jun Chang, Ying Zhu, Xun Sun
One new bifuran derivative (1), together with fourteen known compounds, were isolated from Cordyceps cicadae X. Q. Shing. The known compounds included nine nucleosides, uracil (2), uridine (3), 2'-deoxyuridine (4), 2'-deoxyinosine (5), guanosine (6), 2'-deoxyguanosine (7), thymidine (8), adenosine (9), and 2'-deoxyadenosine (10); three amino acids tryptophan (11), phenylalanine (12), and tyrosine (13); and two dopamine analogues N-acetylnoradrenaline (14) and its dimer, trans-2-(3',4'-dihydroxyphenyl)-3-acetylamino-7-(N-acetyl-2"-amino-ethylene)-1,4-benzodioxane (15)...
December 2015: Natural Product Communications
Marta López-García, María Sonia Dopico García, José Manuel López Vilariño, María Victoria González Rodríguez
In this work MALDI-TOF mass spectroscopy was investigated to characterise the β-glucan profiles of several commercial health supplements, without any derivatisation or purification pre-treatment. The effect of two solvents (water and dimethyl sulfoxide) and two MALDI matrices (2,5-dihydroxybenzoic acid and 2',4',6'-trihydroxyacetophenone) was first evaluated on dextran standards. MALDI-TOF was found as a useful and quick technique to obtain structural information of diverse food supplements based on mushroom extracts...
May 15, 2016: Food Chemistry
Yongjie Zhang, Lihong Guo, Shu Zhang, Xingzhong Liu
OBJECTIVE: To screen efficient molecular markers suitable for genetic diversity analysis of Cordyceps militaris from mitochondrial DNA. METHODS: We amplified 12 mitochondrial DNA fragments and 3 nuclear DNA fragments from each of 20 C. militaris isolates and analyzed nucleotide variations on these DNA fragments. RESULTS: We revealed a greatly higher genetic variation in mitochondrial DNA fragments than in nuclear DNA fragments. Specifically, C...
July 4, 2015: Wei Sheng Wu Xue Bao, Acta Microbiologica Sinica
Masood Shah Khan, Rabea Parveen, Kshipra Mishra, Rajkumar Tulsawani, Sayeed Ahmad
BACKGROUND: Nucleosides are supportive in the regulation and modulation of various physiological processes in body, they acts as precursors in nucleic acid synthesis, enhance immune response, help in absorption of iron and influence the metabolism of fatty acids. Cordyceps sinensis and Ganoderma lucidum are well-known for its use in traditional medicine of China, Nepal and India. They are rich in nucleosides such as adenine, adenosine, cordycepin, etc. Hence, a simple, economic and accurate high-performance liquid chromatography (HPLC) analytical method was proposed for determination of adenine and adenosine for the quality control of plants...
October 2015: Journal of Pharmacy & Bioallied Sciences
Adriano Reis Lucheta, Fabiana de Souza Cannavan, Luiz Fernando Wurdig Roesch, Siu Mui Tsai, Eiko Eurya Kuramae
Here, we compare the fungal community composition and diversity in Amazonian Dark Earth (ADE) and the respective non-anthropogenic origin adjacent (ADJ) soils from four different sites in Brazilian Central Amazon using pyrosequencing of 18S ribosomal RNA (rRNA) gene. Fungal community composition in ADE soils were more similar to each other than their ADJ soils, except for only one site. Phosphorus and aluminum saturation were the main soil chemical factors contributing to ADE and ADJ fungal community dissimilarities...
May 2016: Microbial Ecology
Sapan Kumar Sharma, Nandini Gautam, Narender Singh Atri
In this study, using standard methods, mycelial nutrients, bioactive compounds, and antioxidants were analyzed for the first time for five fungal species: Isaria sinclairii (Berk.) Lloyd, I. tenuipes Peck, I. japonica Yasuda, I. farinosa (Holmsk) Fr. and Cordyceps tuberculata (Lebert) Maire. All of these species were low in fat content and rich in protein, fiber, ash, and carbohydrates. Mineral elements (Fe, Mg, Cu, Mn, and Ca) were detected in appreciable amounts. All three types of fatty acids (saturated, monounsaturated, and polyunsaturated) as well as bioactive compounds (ascorbic acid, β-carotene, lycopene, phenolic compounds, and polysaccharides) were detected for each species...
2015: International Journal of Medicinal Mushrooms
Jannie Siew Lee Chan, Gayane S Barseghyan, Mikheil D Asatiani, Solomon P Wasser
In this paper, we report the results of a proximate analysis (i.e., moisture, ash, protein, fat, carbohydrates, and energy); a bioactive compounds analysis (i.e., cordycepin and ergothioneine); fatty and amino acid analysis; and analyses of vitamin content, macro- and microelement composition of fruiting body (FB), and mycelial biomass (MB) of medicinal caterpillar fungus Cordyceps militaris strain CBS-132098. These results demonstrate that the FB and MB of C. militaris are good sources of proteins: 59.8% protein content in the FB and 39...
2015: International Journal of Medicinal Mushrooms
Tao Hong, Minghua Zhang, Junming Fan
BACKGROUND: Kidney transplantation is the treatment of choice for patients with end-stage kidney disease (ESKD). Rising ESKD prevalence has substantially increased numbers of kidney transplants performed. Maintenance immunosuppression is long-term treatment to prevent acute rejection and deterioration of graft function. Although immunosuppressive treatment using drugs such as calcineurin inhibitors (CNIs, such as cyclosporin A (CsA) or tacrolimus) reduce acute rejection rates, long-term allograft survival rates are not significantly enhanced...
2015: Cochrane Database of Systematic Reviews
Tomohiro Suzuki, Yoshino Takeshima, Toshiyuki Mikamoto, Jun-David Saeki, Tatsuya Kato, Enoch Y Park, Hirokazu Kawagishi, Hideo Dohra
We discovered a novel iflavirus from the transcriptome of the Bombyx mori pupa inoculated with the insect-pathogenic fungus Cordyceps militaris. The assembled iflavirus genome has 10,119 nucleotides, with a 3'-polyadenylated tail, and it encodes a polyprotein composed of 3,004 amino acids.
2015: Genome Announcements
Jingjing Song, Yingwu Wang, Meiyu Teng, Guangsheng Cai, Hongkai Xu, Hanxiao Guo, Yang Liu, Di Wang, Lesheng Teng
Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities. Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. Two week CM administration significantly delayed fatigue phenomenon which is confirmed via rotating rod test, forced swimming test and forced running test. Compared to nontreated mouse, CM administration increased ATP levels and antioxidative enzymes activity and reduced the levels of lactic acid, lactic dehydrogenase, malondialdehyde, and reactive oxygen species...
2015: Evidence-based Complementary and Alternative Medicine: ECAM
Ju Young Yoon, Ji Hye Kim, Kwang-Soo Baek, Geum Soog Kim, Seung Eun Lee, Dae Young Lee, Je Hun Choi, Seung Yu Kim, Hyun Bong Park, Gi-Ho Sung, Kang Ro Lee, Jae Youl Cho, Hyung Jun Noh
BACKGROUND: Cordyceps militaris is one of well-known medicinal mushrooms with anti-inflammatory, anti-cancer, anti-diabetic, and anti-obesity activities. OBJECTIVE: The objective of the following study is to isolate chemical components from the ethanol extract (Cm-EE) from Cordyceps militaris and to evaluate their anti-inflammatory activities. MATERIALS AND METHODS: Column chromatographic separation was performed and anti-inflammatory roles of these compounds were also examined by using NO production and protein kinase B (AKT) activity assays...
July 2015: Pharmacognosy Magazine
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