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cordycepin

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https://www.readbyqxmd.com/read/28099944/cordycepin-induces-apoptosis-by-caveolin-1-mediated-jnk-regulation-of-foxo3a-in-human-lung-adenocarcinoma
#1
Jong Cheon Joo, Jung Hoo Hwang, Eunbi Jo, Young-Rang Kim, Dae Joon Kim, Kyung-Bok Lee, Soo Jung Park, Ik-Soon Jang
Forkhead transcription factor (Foxo3a) is a downstream effector of JNK-induced tumor suppression. However, it is not clear whether the caveolin-1 (CAV1)-mediated JNK/Foxo3a pathway is involved in cancer cell apoptosis. We found that cordycepin upregulates CAV1 expression, which was accompanied by JNK phosphorylation (p-JNK) and subsequent Foxo3a translocation into the nucleus, resulting in the upregulation of Bax protein expression. Furthermore, we found that CAV1 overexpression upregulated p-JNK, whereas CAV1 siRNA downregulated p-JNK...
January 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28074332/functional-analysis-of-ribonucleotide-reductase-from-cordyceps-militaris-expressed-in-escherichia-coli
#2
Tatsuya Kato, Suparmin Ahmad, Enoch Y Park
Cordyceps militaris produces cordycepin (3'-deoxyadenosine), which has various activities, including anti-oxidant, anti-tumoral, anti-viral, and anti-inflammatory. Ribonucleotide reductase (RNR) seems to be a candidate to produce cordycepin in C. militaris because RNR catalyzes the reduction of nucleotides to 2'-deoxynucleotides, whose structures are similar to that of cordycepin. However, the role of RNR has not been confirmed yet. In this study, complementary DNAs (cDNAs) of C. militaris RNR (CmRNR) large and small subunits (CmR1 and CmR2) were cloned from C...
January 10, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28068557/cordycepin-inhibits-migration-of-human-glioblastoma-cells-by-affecting-lysosomal-degradation-and-protein-phosphatase-activation
#3
Dueng-Yuan Hueng, Ching-Hsuan Hsieh, Yu-Chen Cheng, Wen-Chiuan Tsai, Ying Chen
Cordycepin, a nucleoside-derivative-isolated form Cordyceps militaris, has been reported to suppress tumor cell proliferation and cause apoptosis. This study investigates the effect of cordycepin on the migration of human glioblastoma cells. Cordycepin suppressed the migration of the human glioblastoma cell lines U87MG and LN229 in transwell and wound healing assays. Cordycepin decreased protein expression of integrin α1, focal adhesion kinase (FAK), p-FAK, paxillin and p-paxillin. The lysosomal inhibitor NH4Cl blocked the ability of cordycepin to inhibit focal adhesion protein expression and glioma cell migration...
December 29, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28035061/cordycepin-induces-autophagy-mediated-c-flipl-degradation-and-leads-to-apoptosis-in-human-non-small-cell-lung-cancer-cells
#4
Xinghui Yu, Jianya Ling, Xianfang Liu, Sen Guo, Yidan Lin, Xiangguo Liu, Ling Su
Cordycepin, a main active composition extracted from Cordyceps militaris, has been reported to exert anti-tumor activity in a broad spectrum of cancer types. However, the function of cordycepin on human non-small cell lung cancer cells is still obscure. Our present work showed that cordycepin inhibited cell growth by inducing apoptosis and autophagy in human NSCLC cells. Further study revealed that cordycepin triggered extrinsic apoptosis associated with down-regulation of c-FLIPL which suppresses the activity of caspase-8...
December 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27988284/anti-effects-of-cordycepin-to-hypoxia-induced-membrane-depolarization-on-hippocampal-ca1-pyramidal-neuron
#5
Chong Chen, Xin-Ping Liu, Wei Jiang, Bin Zeng, Wei Meng, Li-Ping Huang, Yu-Ping Li, Wei Sun, Chun-Hua Yuan, Li-Hua Yao
Cordycepin has important neuroprotective effects in hypoxic or ischemic insult. However, the direct electrophysiological evidence of cordycepin's neuroprotection from hypoxic or ischemic insult remains unknown. Hence, in this study, the electrophysiological mechanism by which cordycepin protects against ischemic and hypoxic damages has been studied using an energy-deprivation injury model through whole-cell patch clamp techniques. Results revealed that cordycepin (80µM) significantly delayed hypoxia-induced membrane depolarization, including cordycepin reduced slope, and extended the duration of slow depolarization, prolonged the ability to generate spontaneous action potential (AP) firing, delayed the onset of rapid depolarization, and maintained the more hyperpolarized membrane potential after rapid depolarization...
December 15, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27966445/antimetastatic-effects-of-cordycepin-mediated-by-the-inhibition-of-mitochondrial-activity-and-estrogen-related-receptor-%C3%AE-in-human-ovarian-carcinoma-cells
#6
Chia-Woei Wang, Wei-Hsuan Hsu, Chen-Jei Tai
Cordycepin (3'-deoxyadenosine) is a compound for antitumor, which has been found to exert antiangiogenic, antimetastatic, and antiproliferative effects, as well as inducing apoptosis. However, the association between cancer metastasis and mitochondrial activity in cordycepin-treated ovarian carcinoma cells remains unclear. The 50 and 100 μM of cordycepin inhibits mitochondrial fusion and induces mitochondrial fission, respectively. These suggested that cordycepin showed the down-regulation of mitochondrial function and limitation of energy production...
December 9, 2016: Oncotarget
https://www.readbyqxmd.com/read/27927214/antiplatelet-and-antithrombotic-effects-of-cordycepin-enriched-wib-801ce-from-cordyceps-militaris-ex-vivo-in-vivo-and-in-vitro
#7
Hyuk-Woo Kwon, Jung-Hae Shin, Deok Hwi Lim, Woo Jeong Ok, Gi Suk Nam, Min Ji Kim, Ho-Kyun Kwon, Jun-Hee Noh, Je-Young Lee, Hyun-Hong Kim, Jong-Lae Kim, Hwa-Jin Park
BACKGROUND: A species of the fungal genus Cordyceps has been used as a complementary and alternative medicine of traditional Chinese medicine, and its major component cordycepin and cordycepin-enriched WIB-801CE are known to have antiplatelet effects in vitro. However, it is unknown whether they have also endogenous antiplatelet and antithrombotic effects. In this study, to resolve these doubts, we prepared cordycepin-enriched WIB-801CE, an ethanol extract from Cordyceps militaris-hypha, then evaluated its ex vivo, in vivo, and in vitro antiplatelet and antithrombotic effects...
December 7, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27926509/photo-crosslinked-hama-hydrogel-with-cordycepin-encapsulated-chitosan-microspheres-for-osteoarthritis-treatment
#8
Chen Xia, Pengfei Chen, Sheng Mei, Lei Ning, Chenyang Lei, Jiying Wang, Jianfeng Zhang, Jianjun Ma, Shunwu Fan
Autophagy is a protective mechanism in normal cartilage. The present study aimed to investigate the synergistic therapeutic effect of promotion of chondrocyte autophagy via exposure to cordycepin encapsulated by chitosan microspheres (CM-cordycepin) and photo-crosslinked hyaluronic acid methacrylate (HAMA) hydrogel, with the goal of evaluating CM-cordycepin as a treatment for patients with osteoarthritis. First, we developed and evaluated the characteristics of HAMA hydrogels and chitosan microspheres. Next, we measured the effect of cordycepin on cartilage matrix degradation induced by IL1-β in chondrocytes and an ex vivo model...
December 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27910759/acidified-hot-water-extraction-of-adenosine-cordycepin-and-polysaccharides-from-the-chinese-caterpillar-mushroom-ophiocordyceps-sinensis-cs1197-ascomycetes-application-of-an-artificial-neural-network-and-evaluation-of-antioxidant-and-antibacterial-activities
#9
M G Shashidhar, B Manohar
The effects of process variables (temperature, time, and pH) on the extraction of adenosine, cordycepin, and polysaccharides from Ophiocordyceps sinensis CS1197 were studied using response surface methodology (RSM) and an artificial neural network (ANN). The ANN model resulted in low root mean square errors (0.022, 0.079, 0.018) and high R2 values (0.995, 0.934, 0.997) for adenosine, cordycepin, and polysaccharide yields, respectively, which implied good agreement between the predicted and actual data. An overall desirability of 0...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27747890/evaluation-of-different-agricultural-wastes-for-the-production-of-fruiting-bodies-and-bioactive-compounds-by-medicinal-mushroom-cordyceps-militaris
#10
Qunying Lin, Liangkun Long, Liangliang Wu, Fenglun Zhang, Shuling Wu, Weiming Zhang, Xiaoming Sun
BACKGROUND: In commercial production of Cordyceps militaris (a famous Chinese medicine), usually cereal grains were utilized as cultivation substrates. This study is aimed to evaluate the efficiency of agricultural wastes as substitute materials in the low cost production of C. militaris. Cottonseed shells (CS), corn cob particles (CCP), Italian poplar sawdusts (IPS) and substrates spent by Flammulina velutipes (SS) were employed to cultivate C. militaris, using rice medium as control...
October 17, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27689974/cordycepin-induces-apoptosis-and-inhibits-proliferation-of-human-lung-cancer-cell-line-h1975-via-inhibiting-the-phosphorylation-of-egfr
#11
Zheng Wang, Xue Wu, Yan-Ni Liang, Li Wang, Zhong-Xing Song, Jian-Li Liu, Zhi-Shu Tang
Cordycepin is an active component of the traditional Chinese medicine Cordyceps sinensis and Cordyceps militaris with notable anticancer activity. Though the prominent inhibitory activity was reported in different kinds of cancer cell lines, the concrete mechanisms remain elusive. It was reported that cordycepin could be converted into tri-phosphates in vivo to confuse a number of enzymes and interfere the normal cell function. For the inhibitory mechanism of EGFR inhibitors and the structure similarity of ATP and tri-phosphated cordycepin, human lung cancer cell line H1975 was employed to investigate the inhibitory effect of cordycepin...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27649728/effects-of-illumination-pattern-during-cultivation-of-fruiting-body-and-bioactive-compound-production-by-the-caterpillar-medicinal-mushroom-cordyceps-militaris-ascomycetes
#12
Chiu-Yeh Wu, Zeng-Chin Liang, Chin-Yin Tseng, Shu-Hui Hu
We investigated the effects of light intensity in the 3 cultivation stages separately-the mycelium colonization stage, the primordial initiation stage, and the fruiting stage (in order)-on fruiting body and bioactive compound production by Cordyceps militaris. In the mycelium colonization stage, rice substrates were incubated in a spawn running room at 23°C. During the primordial initiation stage, C. militaris was grown at 18°C and illuminated 12 hours/day. In the fruiting stage the temperature was 23°C, with illumination provided 12 hours/day...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27648363/cordycepin-promotes-apoptosis-by-modulating-the-erk-jnk-signaling-pathway-via-dusp5-in-renal-cancer-cells
#13
Jung-Hoo Hwang, Jong Cheon Joo, Dae Joon Kim, Eunbi Jo, Hwa-Seung Yoo, Kyung-Bok Lee, Soo Jung Park, Ik-Soon Jang
Constitutive activation of extracellular signal regulated kinase (ERK)-Jun NH2-terminal kinase (JNK) signaling commonly occurs in tumors. The activation of ERK promotes cell proliferation, whereas that of JNK induces cell apoptosis. However, the apoptotic mechanism of ERK-JNK signaling in cancer is not well understood. Recently, we identified that apoptosis and activation of the JNK signaling pathway were induced after cordycepin treatment in human renal cancer, suggesting that JNK signaling might contribute to TK-10 cell apoptosis...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27646481/cordycepin-attenuates-traumatic-brain-injury-induced-impairments-of-blood-brain-barrier-integrity-in-rats
#14
Jing Yuan, Aihua Wang, Yan He, Zhihua Si, Shan Xu, Shanchao Zhang, Kun Wang, Dawei Wang, Yiming Liu
Loss of blood-brain barrier (BBB) integrity is a downstream event caused by traumatic brain injury (TBI). BBB integrity is affected by certain physiological conditions, including inflammation and oxidative stress. Cordycepin is a susbtance with anti-inflammatory and anti-oxidative effects. Therefore, it is necessary to investigate whether cordycepin affects TBI-induced impairments of BBB integrity. Using TBI rats as the in vivo model and applying multiple techniques, including stroke severity evaluation, Evans blue assessment, quantitative real-time PCR, Western blotting and ELISA, we investigated the dose-dependent protective effects of cordycepin on the TBI-induced impairments of BBB integrity...
September 16, 2016: Brain Research Bulletin
https://www.readbyqxmd.com/read/27588098/separation-of-cordycepin-from-cordyceps-militaris-fermentation-supernatant-using-preparative-hplc-and-evaluation-of-its-antibacterial-activity-as-an-nad-dependent-dna-ligase-inhibitor
#15
Xiaofeng Zhou, Guoqiang Cai, Yi He, Guotong Tong
Cordycepin exhibits various bio-activities, including anticancer, antibacterial, antiviral and immune regulation activities, and is a significant focus of research. However, the preparation of high-purity cordycepin remains challenging. Also, the molecular target with which cordycepin interacts to cause an antibacterial effect remains unknown. In the present study, cordycepin was prepared by preparative high-performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large-scale isolation of cordycepin in the future...
September 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27567378/preparative-isolation-of-cordycepin-n-6-2-hydroxyethyl-adenosine-and-adenosine-from-cordyceps-militaris-by-macroporous-resin-and-purification-by-recycling-high-speed-counter-current-chromatography
#16
Zhong Zhang, Tuernisan Tudi, Yanfang Liu, Shuai Zhou, Na Feng, Yan Yang, Chuanhong Tang, Qingjiu Tang, Jingsong Zhang
In this study, cordycepin, N(6)-(2-hydroxyethyl)-adenosine (HEA) and adenosine from the fruiting bodies of Cordyceps militaris were separated by using macroporous resin NKA-II adsorption. The parameters of static adsorption were tested and the optimized conditions were as follow: the total adsorption time was 12h, 50% ethanol was used for desorption and the desorption time was 9h. The crude sample that was prepared by macroporous resin NKA-II contained 3.4% cordycepin, 3.7% HEA and 4.9% adenosine. Then the crude sample was further purified by recycling high-speed counter-current chromatography (HSCCC) with ethyl acetate, n-butanol, 1...
October 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27553852/ophiocordyceps-formosana-improves-hyperglycemia-and-depression-like-behavior-in-an-stz-induced-diabetic-mouse-model
#17
Chao-Wei Huang, Tzu-Wen Hong, Ying-Jing Wang, Ko-Chien Chen, Ju-Chun Pei, Tai-Yuan Chuang, Wen-Sung Lai, Sheng-Hong Tsai, Richard Chu, Wei-Cheng Chen, Lee-Yan Sheen, Satoru Takahashi, Shih-Torng Ding, Tang-Long Shen
BACKGROUND: A newly defined Cordyceps species, Ophiocordyceps formosana (O. formosana) has been implicated in multitudinous bioactivities, including lowering glucose and cholesterol levels and modulating the immune system. However, few literatures demonstrate sufficient evidence to support these proposed functions. Although the use of Cordyceps spp. has been previously addressed to improve insulin insensitivity and improve the detrimental symptoms of depression; its mechanistic nature remains unsettled...
August 24, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27471034/poly-adp-ribose-polymerases-covalently-modify-strand-break-termini-in-dna-fragments-in-vitro
#18
Ibtissam Talhaoui, Natalia A Lebedeva, Gabriella Zarkovic, Christine Saint-Pierre, Mikhail M Kutuzov, Maria V Sukhanova, Bakhyt T Matkarimov, Didier Gasparutto, Murat K Saparbaev, Olga I Lavrik, Alexander A Ishchenko
Poly(ADP-ribose) polymerases (PARPs/ARTDs) use nicotinamide adenine dinucleotide (NAD(+)) to catalyse the synthesis of a long branched poly(ADP-ribose) polymer (PAR) attached to the acceptor amino acid residues of nuclear proteins. PARPs act on single- and double-stranded DNA breaks by recruiting DNA repair factors. Here, in in vitro biochemical experiments, we found that the mammalian PARP1 and PARP2 proteins can directly ADP-ribosylate the termini of DNA oligonucleotides. PARP1 preferentially catalysed covalent attachment of ADP-ribose units to the ends of recessed DNA duplexes containing 3'-cordycepin, 5'- and 3'-phosphate and also to 5'-phosphate of a single-stranded oligonucleotide...
July 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27446383/cordycepin-induces-apoptosis-in-human-liver-cancer-hepg2-cells-through-extrinsic-and-intrinsic-signaling-pathways
#19
Le-Wen Shao, Li-Hua Huang, Sheng Yan, Jian-Di Jin, Shao-Yan Ren
Cordycepin, also termed 3'-deoxyadenosine, is a nucleoside analogue from Cordyceps sinensis and has been reported to demonstrate numerous biological and pharmacological properties. Our previous study illustrated that the anti-tumor effect of cordycepin may be associated with apoptosis. In the present study, the apoptotic effect of cordycepin on HepG2 cells was investigated using 4',6-diamidino-2-phenylindole, tetraethylbenzimidazolylcarbocyanine iodide and propidium iodide staining analysis and flow cytometry...
August 2016: Oncology Letters
https://www.readbyqxmd.com/read/27439604/cordycepin-inhibits-chondrocyte-hypertrophy-of-mesenchymal-stem-cells-through-pi3k-bapx1-and-notch-signaling-pathway
#20
Zhen Cao, Ce Dou, Jianmei Li, Xiangyu Tang, Junyu Xiang, Chunrong Zhao, Lingyu Zhu, Yun Bai, Qiang Xiang, Shiwu Dong
Mesenchymal stem cells (MSCs) are widely used in cartilage tissue engineering to repair articular cartilage defects. However, hypertrophy of chondrocytes derived from MSCs might hinder the stabilization of hyaline cartilage. Thus, it is very important to find a suitable way to maintain the chondrogenic phenotype of chondrocytes. It has been reported that cordycepin has anti-inflammatory and anti-tumor functions. However, the role of cordycepin in chondrocyte hypertrophy remains unclear. Therefore, the objective of this study was to determine the effect of cordycepin on chondrogenesis and chondrocyte hypertrophy in MSCs and ATDC5 cells...
October 2016: BMB Reports
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