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sonication graft infection

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https://www.readbyqxmd.com/read/26725710/resistance-to-infection-of-long-term-cryopreserved-human-aortic-valve-allografts
#1
COMPARATIVE STUDY
Viola Steffen, Georg Marsch, Karin Burgwitz, Christian Kuehn, Omke E Teebken
OBJECTIVE: To analyze the in vitro antimicrobial activity of 3 antibiotic regimens (group A, gentamicin-piperacillin-vancomycin-metronidazole-amphotericin B; group B, gentamicin-piperacillin-flucloxacillin-metronidazole-amphotericin B; and group C, meropenem-vancomycin-tobramycin-colistin-amphotericin B) used in the processing of cryopreserved human ascending aortic tissue and aortic valves against Staphylococcus epidermidis and Staphylococcus aureus. The results were additionally compared with the infection resistance of cryopreserved ascending aortic tissue against Escherichia coli and Pseudomonas aeruginosa...
May 2016: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/26080300/should-white-blood-cell-scan-be-replaced-by-18-f-fdg-pet-ct-in-the-diagnosis-of-prosthetic-vascular-graft-infection
#2
Jean-Baptiste Pinaquy, Xavier Berard, Laurent Stecken, Ghoufrane Tlili, Fatima M'zali, Laurence Bordenave, Sabine Pereyre, Stéphane Mayeux, Charles Cazanave
Diagnosis of prosthetic vascular graft infection (PVGI) is a clinical challenge requiring accurate diagnostic methods for their optimal management. A 65-year-old patient with suspected PVGI was explored by fluorodeoxyglucose positron emission tomography combined with computed tomography ((18)F-FDG PET-CT) for pretreatment staging. Standard imaging was unrevealing but PET images showed multiple foci with increased uptake suggesting prosthetic infection. While routine results from the diagnostic laboratory were negative, prosthesis sonication before standard culture revealed the same bacterium as a culture of preoperative lymphocele aspiration...
August 2015: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/25693675/staphylococcal-biofilm-formation-on-the-surface-of-three-different-calcium-phosphate-bone-grafts-a-qualitative-and-quantitative-in-vivo-analysis
#3
Ulrika Furustrand Tafin, Bertrand Betrisey, Marc Bohner, Thomas Ilchmann, Andrej Trampuz, Martin Clauss
Differences in physico-chemical characteristics of bone grafts to fill bone defects have been demonstrated to influence in vitro bacterial biofilm formation. Aim of the study was to investigate in vivo staphylococcal biofilm formation on different calcium phosphate bone substitutes. A foreign-body guinea-pig infection model was used. Teflon cages prefilled with β-tricalcium phosphate, calcium-deficient hydroxyapatite, or dicalcium phosphate (DCP) scaffold were implanted subcutaneously. Scaffolds were infected with 2 × 10(3) colony-forming unit of Staphylococcus aureus (two strains) or S...
March 2015: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/25036054/influence-of-physico-chemical-material-characteristics-on-staphylococcal-biofilm-formation-a-qualitative-and-quantitative-in-vitro-analysis-of-five-different-calcium-phosphate-bone-grafts
#4
COMPARATIVE STUDY
M Clauss, U Furustrand Tafin, B Betrisey, N van Garderen, A Trampuz, T Ilchmann, M Bohner
Various compositions of synthetic calcium phosphates (CaP) have been proposed and their use has considerably increased over the past decades. Besides differences in physico-chemical properties, resorption and osseointegration, artificial CaP bone graft might differ in their resistance against biofilm formation. We investigated standardised cylinders of 5 different CaP bone grafts (cyclOS, chronOS (both β-TCP (tricalcium phosphate)), dicalcium phosphate (DCP), calcium-deficient hydroxyapatite (CDHA) and α-TCP)...
2014: European Cells & Materials
https://www.readbyqxmd.com/read/24145635/evaluation-of-microbial-flora-in-eyes-with-a-boston-type-1-keratoprosthesis
#5
Samuel H Lee, Mark J Mannis, Brett Shapiro, Jennifer Y Li, Christopher Polage, Wayne Smith
PURPOSE: To evaluate the microbial flora of eyes with a Boston Keratoprosthesis (K-Pro). METHODS: A prospective study was performed for 17 eyes of 15 patients who underwent a K-Pro implantation between September 2005 and June 2011. Preoperative diagnoses included failed corneal grafts, limbal stem cell deficiency, chemical burns, and Stevens-Johnson Syndrome. The patients used topical antibiotics after their surgery including a fluoroquinolone, polymyxin-trimethoprim, vancomycin, or a combination of the 3...
December 2013: Cornea
https://www.readbyqxmd.com/read/23965956/the-efficacy-of-the-quorum-sensing-inhibitor-fs8-and-tigecycline-in-preventing-prosthesis-biofilm-in-an-animal-model-of-staphylococcal-infection
#6
Oriana Simonetti, Oscar Cirioni, Federico Mocchegiani, Ivana Cacciatore, Carmela Silvestri, Leonardo Baldassarre, Fiorenza Orlando, Pamela Castelli, Mauro Provinciali, Marco Vivarelli, Erika Fornasari, Andrea Giacometti, Annamaria Offidani
We investigated the efficacy of tigecycline and FS8, alone or combined, in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2 x 107 colony-forming units of Staphylococcus aureus, strain Smith diffuse. The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis, and three contaminated groups that received: (i) intraperitoneal tigecycline, (ii) FS8-soaked graft, and (iii) tigecycline plus FS8-soaked graft, respectively...
2013: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/23262356/quorum-sensing-inhibitor-fs3-coated-vascular-graft-enhances-daptomycin-efficacy-in-a-rat-model-of-staphylococcal-infection
#7
Oscar Cirioni, Federico Mocchegiani, Ivana Cacciatore, Jacopo Vecchiet, Carmela Silvestri, Leonardo Baldassarre, Claudio Ucciferri, Elena Orsetti, Pamela Castelli, Mauro Provinciali, Marco Vivarelli, Erika Fornasari, Andrea Giacometti
The aim of the study was to investigate the efficacy of the quorum sensing inhibitor FS3 and daptomycin in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2×10(7) colony-forming units of Staphylococcus aureus, strain Smith diffuse. The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis and three contaminated groups that received: (i) intraperitoneal daptomycin, (ii) FS3-soacked graft, and (iii) daptomycin plus FS3-soaked graft, respectively...
February 2013: Peptides
https://www.readbyqxmd.com/read/22093165/microbial-ureteral-stent-colonization-in-renal-transplant-recipients-frequency-and-influence-on-the-short-time-functional-outcome
#8
G Bonkat, M Rieken, F P Siegel, R Frei, J Steiger, I Gröschl, T C Gasser, S Dell-Kuster, R Rosenthal, L Gürke, S Wyler, A Bachmann, A F Widmer
Ureteral stent insertion at the time of renal transplantation significantly decreases complications of urine leakage and obstruction, but bears an intrinsic risk of microbial colonization. Associated urinary tract infection (UTI) may pose a significant risk for graft infection and subsequent graft failure, in particular, during high-level immunosuppression in the early phase after transplantation. The aims of this prospective study were (i) to assess the frequency of microbial ureteral stent colonization (MUSC) in renal transplant recipients by sonication, (ii) to compare the diagnostic value of sonication with that of conventional urine culture (CUC), (iii) to determine biofilm forming organisms, and (iv) to investigate the influence of MUSC on the short-time functional outcome...
February 2012: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/20869272/daptomycin-and-rifampin-alone-and-in-combination-prevent-vascular-graft-biofilm-formation-and-emergence-of-antibiotic-resistance-in-a-subcutaneous-rat-pouch-model-of-staphylococcal-infection
#9
O Cirioni, F Mocchegiani, R Ghiselli, C Silvestri, E Gabrielli, E Marchionni, F Orlando, D Nicolini, A Risaliti, A Giacometti
OBJECTIVE: To investigate the efficacy of daptomycin and rifampin either alone or in combination in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. DESIGN: Prospective, randomised, controlled animal study. MATERIALS: Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2×10(7) colony forming units of Staphylococcus aureus, strain Smith diffuse...
December 2010: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/20394708/comparison-of-anti-bacterial-prophylactic-properties-of-two-different-vascular-grafts-action-of-anti-bacterial-graft-coating-and-systemic-antibiotic-treatment
#10
COMPARATIVE STUDY
M Artini, G L Scoarughi, R Papa, A Cellini, C Avanzolini, E Mileto, L Rizzo, L Selan
The main problem associated with artificial vascular devices is the risk of bacterial infections, mostly sustained by coagulase negative Staphylococci or Staphylococcus aureus. Many efforts have been made to identify materials refractory to bacterial adhesion. The aim of our study is to verify the antimicrobial properties of two kinds of vascular prosthesis to prevent early onset infections and the efficacy of the concomitant action of a systemic antibiotic treatment. Adult male Wistar rats were used. We subcutaneously implanted in four groups a silver-coated prosthesis fragment, and a rifampicin-soaked prosthesis fragment in the remaining four groups...
January 2010: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/20362612/bacterial-translocation-across-eptfe-vascular-graft-surfaces
#11
Supriya Narasimhan, Saima Aslam, Peter H Lin, Carlos F Bechara, Mohammad D Mansouri, Rabih O Darouiche
OBJECTIVES: Vascular graft infections arise from bacterial colonization of either the external or internal graft surfaces. We assessed whether methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli could translocate through pores of ePTFE grafts. METHODS: To assess translocation from the internal to the external surface, we placed 10(5) cfu of bacterial suspension inside ePTFE graft segments and suspended them in sterile broth for 72 h. To assess translocation from the external to the internal surface, we placed sterile broth inside ePTFE segments, and incubated them for 72 h in a bacterial suspension (10(5) cfu/mL)...
June 2010: Journal of Infection
https://www.readbyqxmd.com/read/17537542/the-lipopeptides-pal-lys-lys-nh-2-and-pal-lys-lys-soaking-alone-and-in-combination-with-intraperitoneal-vancomycin-prevent-vascular-graft-biofilm-in-a-subcutaneous-rat-pouch-model-of-staphylococcal-infection
#12
COMPARATIVE STUDY
Oscar Cirioni, Andrea Giacometti, Roberto Ghiselli, Wojciech Kamysz, Carmela Silvestri, Fiorenza Orlando, Federico Mocchegiani, Agnese Della Vittoria, Elzbieta Kamysz, Vittorio Saba, Giorgio Scalise
Staphylococcal infections are often associated with the use of implantable medical devices. Such infections are difficult to treat because of biofilm resistance to antibiotics and are common causes of morbidity and mortality. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2x10(7) colony-forming units of bacterial strains. The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis and five contaminated groups that received intraperitoneal vancomycin, Pal-Lys-Lys-NH(2) and Pal-Lys-Lys-soacked graft, and vancomycin plus Pal-Lys-Lys-NH(2) or Pal-Lys-Lys-soacked graft, respectively...
June 2007: Peptides
https://www.readbyqxmd.com/read/17481929/vascular-graft-infection-by-staphylococcus-aureus-efficacy-of-cefazolin-teicoplanin-and-vancomycin-prophylaxis-protocols-in-a-rat-model
#13
COMPARATIVE STUDY
E Atahan, M Gul, Y Ergun, E Eroglu
OBJECTIVES: Prophylactic efficiencies of cefazolin, teicoplanin and vancomycin in a dacron graft infection model caused by methicillin-susceptible (MSSA) or -resistant Staphylococcus aureus (MRSA) were investigated. DESIGN: Prospective, randomized, controlled animal study. MATERIALS AND METHODS: Infections were established subcutaneously in the back of rats by implantation of Dacron prostheses followed by topical inoculation onto grafts of MSSA or MRSA...
August 2007: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/16725356/gelatin-sealed-dacron-graft-is-not-more-susceptible-to-mrsa-infection-than-ptfe-graft
#14
COMPARATIVE STUDY
A Yasim, M Gul, H Ciralik, Y Ergun
OBJECTIVES: The purpose of this experimental study was to compare the susceptibility of gelatin-sealed Dacron and PTFE prostheses to infection by MRSA. DESIGN: Prospective, randomized, controlled animal study. MATERIALS AND METHODS: Graft infections were established in the subcutaneous tissues of 60 female Spraque-Dawley rats by the implantation of gelatin-sealed Dacron or PTFE prostheses followed by topical inoculation with methicillin-resistant Staphylococcus aureus...
October 2006: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/16360378/the-prophylactic-efficacy-of-rifampicin-soaked-graft-in-combination-with-systemic-vancomycin-in-the-prevention-of-prosthetic-vascular-graft-infection-an-experimental-study
#15
Mustafa Sacar, Ibrahim Goksin, Ahmet Baltalarli, Huseyin Turgut, Suzan Sacar, Gokhan Onem, Vefa Ozcan, Fahri Adali
OBJECTIVE: To investigate the prophylactic efficacy of systemic, topical, or combined antibiotic usage in the prevention of late prosthetic vascular graft infection caused by methicillin-resistant Staphylococcus epidermidis (MRSE) and the differential adherence of S. epidermidis to Dacron and ePTFE grafts in a rat model. MATERIALS AND METHODS: Graft infections were established in the back subcutaneous tissue of 120 adult male Wistar rats by implantation of 1-cm(2) Dacron/ePTFE prosthesis followed by topical inoculation with 2 x 10(7) CFU of clinical isolate of MRSE...
December 2005: Journal of Surgical Research
https://www.readbyqxmd.com/read/15256945/processing-of-whole-femoral-head-allografts-a-method-for-improving-clinical-efficacy-and-safety
#16
R Lomas, O Drummond, J N Kearney
Femoral heads removed during primary hip replacement surgery are widely utilised as a source of allograft bone. Despite evidence that processing these grafts to remove blood and marrow elements improves both the clinical performance and safety of these allografts, many are transplanted without any processing being applied at all. The goal of this study was to investigate the efficiency of an allograft processing protocol which incorporates pasteurisation, (3 h, 56-60 degrees C) centrifugation, (1850g, 2 x 15 min, 40 degrees C) sonication, and repeated washing in warm (56-60 degrees C, 19 h) distilled, sterile water to remove blood and marrow elements from the graft...
2000: Cell and Tissue Banking
https://www.readbyqxmd.com/read/11891733/laser-induced-shock-waves-enhance-sterilization-of-infected-vascular-prosthetic-grafts
#17
G R Nigri, S Tsai, S Kossodo, P Waterman, P Fungaloi, D C Hooper, A G Doukas, G M LaMuraglia
BACKGROUND AND OBJECTIVE: Bacteria that cause infection of vascular prosthetic grafts produce an exopolysaccharide matrix known as biofilm. Growth in biofilms protects the bacteria from leukocytes, antibodies and antimicrobial drugs. Laser-generated shock waves (SW) can disrupt biofilms and increase drug penetration. This study investigates the possibility of increasing antibiotic delivery and sterilization of vascular prosthetic graft. STUDY DESIGN/MATERIALS AND METHODS: Strains of Staphylococcus epidermidis and S...
2001: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/11469904/prophylaxis-against-staphylococcus-aureus-vascular-graft-infection-with-mupirocin-soaked-collagen-sealed-dacron
#18
COMPARATIVE STUDY
R Ghiselli, A Giacometti, L Goffi, O Cirioni, F Mocchegiani, F Orlando, A M Paggi, E Petrelli, G Scalise, V Saba
A rat model was used to investigate the efficacy of mupirocin in the prevention of vascular prosthetic graft infections. The effect of mupirocin-soaked Dacron was compared with the effect of rifampin-soaked, collagen-sealed Dacron in the rat model of graft infection caused by methicillin-susceptible Staphylococcus aureus and methicillin-resistant S. aureus. Graft infections were established in the back subcutaneous tissue of 195 adult male Wistar rats by implantation of 1-cm(2) Dacron prostheses followed by topical inoculation with 5 x 10(7) colony-forming units of S...
August 2001: Journal of Surgical Research
https://www.readbyqxmd.com/read/11096035/is-there-a-role-for-chlamydia-pneumoniae-in-hemodialysis-vascular-access-thrombosis
#19
A W Zydlewski, D R Stothard, B Van Der Pol, R B Jones, R S Filo, J A Hasbargen
Significant evidence suggests that Chlamydia pneumoniae has a major role in occlusive vascular disease. Vascular access thrombosis in chronic hemodialysis patients is a frequent problem; the underlying pathological state is stenosis caused by endothelial hyperplasia. There is presently no literature concerning C pneumoniae in vascular access thrombosis. We embarked on a study to evaluate the possible role of C pneumoniae in access failure. Ten consecutive patients with thrombosed polytetrafluoroethylene (PTFE) conduit arteriovenous fistulae undergoing surgical thrombectomy and revision were studied...
December 2000: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
https://www.readbyqxmd.com/read/11083632/polycationic-peptides-as-prophylactic-agents-against-methicillin-susceptible-or-methicillin-resistant-staphylococcus-epidermidis-vascular-graft-infection
#20
A Giacometti, O Cirioni, R Ghiselli, L Goffi, F Mocchegiani, A Riva, G Scalise, V Saba
Several polycationic peptides isolated from animals, plants, and bacterial species possess a broad spectrum of antimicrobial activity. A rat model was used to investigate the efficacies of two peptides, ranalexin and buforin II, in the prevention of vascular prosthetic graft infections. The effect of peptide-soaked collagen-sealed Dacron was compared to that of rifampin-soaked collagen-sealed Dacron in the rat model of graft infection caused by methicillin-susceptible rifampin-susceptible Staphylococcus epidermidis and methicillin-resistant rifampin-susceptible S...
December 2000: Antimicrobial Agents and Chemotherapy
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