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Bdellovibrio bacteriovorus

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https://www.readbyqxmd.com/read/27915281/effects-of-predation-and-dispersal-on-bacterial-abundance-and-contaminant-biodegradation
#1
Sally Otto, Hauke Harms, Lukas Y Wick
Research into the biodegradation of soil contaminants has rarely addressed the consequences of predator-prey interactions. Here, we investigated the joint effect of predation and dispersal networks on contaminant degradation by linking spatial abundances of degrader (Pseudomonas fluorescens) and predator (Bdellovibrio bacteriovorus) bacteria to the degradation of the major soil contaminant phenanthrene (PHE). We used a laboratory microcosm with a PHE passive dosing system and a glass fiber network to facilitate bacterial dispersal...
December 2, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27889262/injections-of-predatory-bacteria-work-alongside-host-immune-cells-to-treat-shigella-infection-in-zebrafish-larvae
#2
Alexandra R Willis, Christopher Moore, Maria Mazon-Moya, Sina Krokowski, Carey Lambert, Robert Till, Serge Mostowy, R Elizabeth Sockett
Bdellovibrio bacteriovorus are predatory bacteria that invade and kill a range of Gram-negative bacterial pathogens in natural environments and in vitro [1, 2]. In this study, we investigated Bdellovibrio as an injected, antibacterial treatment in vivo, using zebrafish (Danio rerio) larvae infected with an antibiotic-resistant strain of the human pathogen Shigella flexneri. When injected alone, Bdellovibrio can persist for more than 24 hr in vivo yet exert no pathogenic effects on zebrafish larvae. Bdellovibrio injection of zebrafish containing a lethal dose of Shigella promotes pathogen killing, leading to increased zebrafish survival...
November 22, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27834203/predatory-bacteria-attenuate-klebsiella-pneumoniae-burden-in-rat-lungs
#3
Kenneth Shatzkes, Eric Singleton, Chi Tang, Michael Zuena, Sean Shukla, Shilpi Gupta, Sonal Dharani, Onoyom Onyile, Joseph Rinaggio, Nancy D Connell, Daniel E Kadouri
: Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are predatory bacteria that naturally-and obligately-prey on other Gram-negative bacteria, and their use has been proposed as a potential new approach to control microbial infection. The ability of predatory bacteria to prey on Gram-negative human pathogens in vitro is well documented; however, the in vivo safety and efficacy of predatory bacteria have yet to be fully assessed. In this study, we examined whether predatory bacteria can reduce bacterial burden in the lungs in an in vivo mammalian system...
November 8, 2016: MBio
https://www.readbyqxmd.com/read/27824344/catch-me-if-you-can-dispersal-and-foraging-of-bdellovibrio-bacteriovorus-109j-along-mycelia
#4
Sally Otto, Estelle P Bruni, Hauke Harms, Lukas Y Wick
To cope with heterogeneous environments and resource distributions, filamentous fungi have evolved a spatially extensive growth enabling their hyphae to penetrate air-water interfaces and pass through air-filled pores. Such mycelia are also known to act as dispersal networks for the mobilisation of bacteria ('fungal highways') and connection of microbial microhabitats. Hitherto, however, nothing is known about the effect of mycelia-based dispersal on interactions between bacterial predators and their prey and concomitant effects on biomass formation...
November 8, 2016: ISME Journal
https://www.readbyqxmd.com/read/27629536/investigating-the-responses-of-human-epithelial-cells-to-predatory-bacteria
#5
Ajay K Monnappa, Wasimul Bari, Seong Yeol Choi, Robert J Mitchell
One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrio-and-like-organisms (BALOs), predatory bacteria known to attack human pathogens. Consequently, in this study, the responses from four cell lines (three human and one mouse) were characterized during an exposure to different predatory bacteria, Bdellovibrio bacteriovorus HD100, Bacteriovorus BY1 and Bacteriovorax stolpii EB1. TNF-α levels were induced in Raw 264.7 mouse macrophage cultures with each predator, but paled in comparison to those obtained with E...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27580114/efficacy-of-bdellovibrio-bacteriovorus-109j-for-the-treatment-of-dairy-calves-with-experimentally-induced-infectious-bovine-keratoconjunctivitis
#6
Mélanie J Boileau, Rinosh Mani, Melanie A Breshears, Margi Gilmour, Jared D Taylor, Kenneth D Clinkenbeard
OBJECTIVE To determine the efficacy of Bdellovibrio bacteriovorus 109J for the treatment of calves with experimentally induced infectious bovine keratoconjunctivitis (IBK). ANIMALS 12 healthy dairy calves. PROCEDURES For each calf, a grid keratotomy was performed on both eyes immediately before inoculation with Moraxella bovis hemolytic strain Epp63-300 (n = 11 calves) or nonhemolytic strain 12040577 (1 calf). For each calf inoculated with M bovis Epp63-300, the eyes were randomly assigned to receive an artificial tear solution with (treatment group) or without (control group) lyophilized B bacteriovorus 109J...
September 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27579919/effect-of-predatory-bacteria-on-human-cell-lines
#7
Shilpi Gupta, Chi Tang, Michael Tran, Daniel E Kadouri
Predatory bacteria are Gram-negative bacteria that prey on other Gram-negative bacteria and have been considered as potential therapeutic agents against multi-drug resistant pathogens. In vivo animal models have demonstrated that predatory bacteria are non-toxic and non-immunogenic in rodents. In order to consider the use of predatory bacteria as live antibiotics, it is important to investigate their effect on human cells. The aim of this study was to determine the effect of Bdellovibrio bacteriovorus strains 109J and HD100, and Micavibrio aeruginosavorus strain ARL-13 on cell viability and inflammatory responses of five human cell lines, representative of clinically relevant tissues...
2016: PloS One
https://www.readbyqxmd.com/read/27527833/predatory-bacteria-are-nontoxic-to-the-rabbit-ocular-surface
#8
Eric G Romanowski, Nicholas A Stella, Kimberly M Brothers, Kathleen A Yates, Martha L Funderburgh, James L Funderburgh, Shilpi Gupta, Sonal Dharani, Daniel E Kadouri, Robert M Q Shanks
Given the increasing emergence of antimicrobial resistant microbes and the near absent development of new antibiotic classes, innovative new therapeutic approaches to address this global problem are necessary. The use of predatory bacteria, bacteria that prey upon other bacteria, is gaining interest as an "out of the box" therapeutic treatment for multidrug resistant pathogenic bacterial infections. Before a new antimicrobial agent is used to treat infections, it must be tested for safety. The goal of this study was to test the tolerability of bacteria on the ocular surface using in vitro and in vivo models...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27328268/isolation-of-bdellovibrio-bacteriovorus-from-a-tropical-wastewater-treatment-plant-and-predation-of-mixed-species-biofilms-assembled-by-the-native-community-members
#9
Shugeng Feng, Chuan Hao Tan, Yehuda Cohen, Scott A Rice
It is reported here that a predatory bacterium belonging to the Genus Bdellovibrio, was isolated from activated sludge at the Ulu Pandan Water Reclamation Plant, Singapore. 16S rDNA gene sequencing analysis revealed that this isolate was 99% identical to 'Bdellovibrio bacteriovorus strain Tiberius' and hence is designated as 'Bdellovibrio bacteriovorus UP'. Using a novel approach based on fluorescence in situ hybridization (FISH), a prey cell density-dependent growth pattern of B. bacteriovorus UP was established...
November 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27106259/identification-and-differential-production-of-ubiquinone-8-in-the-bacterial-predator-bdellovibrio-bacteriovorus
#10
Eileen M Spain, Megan E Núñez, Hyeong-Jin Kim, Ryan J Taylor, Nicholas Thomas, Michael B Wengen, Nathan F Dalleska, Joseph P Bromley, Kimberly H Schermerhorn, Megan A Ferguson
Bdellovibrio bacteriovorus 109J, a predatory bacterium with potential as a bacterial control agent, can exist in several lifestyles that differ both in predatory capacity and color. We determined that levels of ubiquinone-8 contribute to the distinctive but variable yellow color of different types of Bdellovibrio cells. Steady-state ubiquinone-8 concentrations did not differ markedly between conventional predatory and host-independent B. bacteriovorus despite upregulation of a suite of ubiquinone-8 synthesis genes in host-independent cells...
June 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27087466/engineering-a-predatory-bacterium-as-a-proficient-killer-agent-for-intracellular-bio-products-recovery-the-case-of-the-polyhydroxyalkanoates
#11
Virginia Martínez, Cristina Herencias, Edouard Jurkevitch, M Auxiliadora Prieto
This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator, or by recombinant Escherichia coli strains. B. bacteriovorus with a mutated PHA depolymerase gene to prevent the unwanted breakdown of the bio-product allowed the recovery of up to 80% of that accumulated by the prey bacteria, even at high biomass concentrations...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26865299/bacterial-predator-prey-dynamics-in-microscale-patchy-landscapes
#12
Felix J H Hol, Or Rotem, Edouard Jurkevitch, Cees Dekker, Daniel A Koster
Soil is a microenvironment with a fragmented (patchy) spatial structure in which many bacterial species interact. Here, we explore the interaction between the predatory bacterium Bdellovibrio bacteriovorus and its prey Escherichia coli in microfabricated landscapes. We ask how fragmentation influences the prey dynamics at the microscale and compare two landscape geometries: a patchy landscape and a continuous landscape. By following the dynamics of prey populations with high spatial and temporal resolution for many generations, we found that the variation in predation rates was twice as large in the patchy landscape and the dynamics was correlated over shorter length scales...
February 10, 2016: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/26792139/erratum-ankyrin-mediated-self-protection-during-cell-invasion-by-the-bacterial-predator-bdellovibrio-bacteriovorus
#13
Carey Lambert, Ian T Cadby, Rob Till, Nhat Khai Bui, Thomas R Lerner, William S Hughes, David J Lee, Luke J Alderwick, Waldemar Vollmer, R Elizabeth Sockett, Andrew L Lovering
No abstract text is available yet for this article.
2016: Nature Communications
https://www.readbyqxmd.com/read/26712556/visualizing-bdellovibrio-bacteriovorus-by-using-the-tdtomato-fluorescent-protein
#14
Somdatta Mukherjee, Kimberly M Brothers, Robert M Q Shanks, Daniel E Kadouri
Bdellovibrio bacteriovorus is a Gram-negative bacterium that belongs to the delta subgroup of proteobacteria and is characterized by a predatory life cycle. In recent years, work has highlighted the potential use of this predator to control bacteria and biofilms. Traditionally, the reduction in prey cells was used to monitor predation dynamics. In this study, we introduced pMQ414, a plasmid that expresses the tdTomato fluorescent reporter protein, into a host-independent strain and a host-dependent strain of B...
March 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/26626559/ankyrin-mediated-self-protection-during-cell-invasion-by-the-bacterial-predator-bdellovibrio-bacteriovorus
#15
Carey Lambert, Ian T Cadby, Rob Till, Nhat Khai Bui, Thomas R Lerner, William S Hughes, David J Lee, Luke J Alderwick, Waldemar Vollmer, R Elizabeth Sockett, Elizabeth R Sockett, Andrew L Lovering
Predatory Bdellovibrio bacteriovorus are natural antimicrobial organisms, killing other bacteria by whole-cell invasion. Self-protection against prey-metabolizing enzymes is important for the evolution of predation. Initial prey entry involves the predator's peptidoglycan DD-endopeptidases, which decrosslink cell walls and prevent wasteful entry by a second predator. Here we identify and characterize a self-protection protein from B. bacteriovorus, Bd3460, which displays an ankyrin-based fold common to intracellular pathogens of eukaryotes...
December 2, 2015: Nature Communications
https://www.readbyqxmd.com/read/26608034/a-bacterial-homologue-of-the-human-iron-exporter-ferroportin
#16
Maria Carmela Bonaccorsi di Patti, Fabio Polticelli, Valentina Tortosa, Pier Antonio Furbetta, Giovanni Musci
A bacterial homologue of the human iron exporter ferroportin found in the predatory Gram-negative bacterium Bdellovibrio bacteriovorus has been investigated. Molecular modelling, expression in recombinant form and iron binding and transport assays demonstrate that B. bacteriovorus ferroportin (bdFpn) is indeed an orthologue of human ferroportin. Key residues corresponding to those essential for iron binding and transport in human ferroportin are conserved in the bacterial homologue and are predicted to be correctly clustered in the central cavity of the protein...
December 21, 2015: FEBS Letters
https://www.readbyqxmd.com/read/26524597/structural-and-enzymatic-characterization-of-a-nucleoside-diphosphate-sugar-hydrolase-from-bdellovibrio-bacteriovorus
#17
Andres H de la Peña, Allison Suarez, Krisna C Duong-Ly, Andrew J Schoeffield, Mario A Pizarro-Dupuy, Melissa Zarr, Silvia A Pineiro, L Mario Amzel, Sandra B Gabelli
Given the broad range of substrates hydrolyzed by Nudix (nucleoside diphosphate linked to X) enzymes, identification of sequence and structural elements that correctly predict a Nudix substrate or characterize a family is key to correctly annotate the myriad of Nudix enzymes. Here, we present the structure determination and characterization of Bd3179 -- a Nudix hydrolase from Bdellovibrio bacteriovorus-that we show localized in the periplasmic space of this obligate Gram-negative predator. We demonstrate that the enzyme is a nucleoside diphosphate sugar hydrolase (NDPSase) and has a high degree of sequence and structural similarity to a canonical ADP-ribose hydrolase and to a nucleoside diphosphate sugar hydrolase (1...
2015: PloS One
https://www.readbyqxmd.com/read/26487679/cell-cycle-progress-in-obligate-predatory-bacteria-is-dependent-upon-sequential-sensing-of-prey-recognition-and-prey-quality-cues
#18
Or Rotem, Zohar Pasternak, Eyal Shimoni, Eduard Belausov, Ziv Porat, Shmuel Pietrokovski, Edouard Jurkevitch
Predators feed on prey to acquire the nutrients necessary to sustain their survival, growth, and replication. In Bdellovibrio bacteriovorus, an obligate predator of Gram-negative bacteria, cell growth and replication are tied to a shift from a motile, free-living phase of search and attack to a sessile, intracellular phase of growth and replication during which a single prey cell is consumed. Engagement and sustenance of growth are achieved through the sensing of two unidentified prey-derived cues. We developed a novel ex vivo cultivation system for B...
November 3, 2015: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26324450/an-extended-cyclic-di-gmp-network-in-the-predatory-bacterium-bdellovibrio-bacteriovorus
#19
Or Rotem, Jutta Nesper, Ilya Borovok, Rena Gorovits, Mikhail Kolot, Zohar Pasternak, Irina Shin, Timo Glatter, Shmuel Pietrokovski, Urs Jenal, Edouard Jurkevitch
UNLABELLED: Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and riboswitches have been extensively studied, relatively few effectors have been identified and most are of unknown functions. The obligate predatory bacterium Bdellovibrio bacteriovorus has a peculiar dimorphic life cycle, in which a phenotypic transition from a free-living attack phase (AP) to a sessile, intracellular predatory growth phase (GP) is tightly regulated by specific c-di-GMP diguanylate cyclases...
January 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/26319730/investigation-into-the-efficacy-of-bdellovibrio-bacteriovorus-as-a-novel-preharvest-intervention-to-control-escherichia-coli-o157-h7-and-salmonella-in-cattle-using-an-in-vitro-model
#20
Jennifer A Page, Brian Lubbers, Joshua Maher, Linda Ritsch, Sara E Gragg
Cattle are an important reservoir for the foodborne pathogens Salmonella and Escherichia coli O157:H7; they frequently harbor these microorganisms in their digestive tracts and shed them in their feces. Thus, there is potential for contamination of cattle hides and, subsequently, carcasses. Interventions aimed at reducing or eliminating pathogen shedding preharvest will also reduce the likelihood of beef product contamination by these pathogens. Therefore, this study used an in vitro model to evaluate Bdellovibrio bacteriovorus, a gram-negative microorganism that preys upon other gram-negative microorganisms, as a preharvest intervention to control Salmonella and E...
September 2015: Journal of Food Protection
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