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Bdellovibrio bacteriovorus

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https://www.readbyqxmd.com/read/28919044/susceptibility-of-colistin-resistant-pathogens-to-predatory-bacteria
#1
Sonal Dharani, Dong Hyun Kim, Robert M Q Shanks, Yohei Doi, Daniel E Kadouri
The increase in multidrug-resistant Gram-negative bacterial infections has forced the reintroduction of antibiotics such as colistin. However, the spread of plasmid-borne mcr-1 colistin resistance genes have moved us closer to an era of untreatable Gram-negative infections. To evaluate whether predatory bacteria could be used as a potential therapeutic to treat this upcoming threat, the ability of Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus to prey on several clinically relevant mcr-1-positive, colistin-resistant isolates was evaluated...
September 14, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28886689/predator-versus-pathogen-how-does-predatory-bdellovibrio-bacteriovorus-interface-with-the-challenges-of-killing-gram-negative-pathogens-in-a-host-setting
#2
David Negus, Chris Moore, Michelle Baker, Dhaarini Raghunathan, Jess Tyson, R Elizabeth Sockett
Bdellovibrio bacteriovorus is a small deltaproteobacterial predator that has evolved to invade, reseal, kill, and digest other gram-negative bacteria in soils and water environments. It has a broad host range and kills many antibiotic-resistant, clinical pathogens in vitro, a potentially useful capability if it could be translated to a clinical setting. We review relevant mechanisms of B. bacteriovorus predation and the physiological properties that would influence its survival in a mammalian host. Bacterial pathogens increasingly display conventional antibiotic resistance by expressing and varying surface and soluble biomolecules...
September 8, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28827526/measuring-and-modelling-the-response-of-klebsiella-pneumoniae-kpc-prey-to-bdellovibrio-bacteriovorus-predation-in-human-serum-and-defined-buffer
#3
Michelle Baker, David Negus, Dhaarini Raghunathan, Paul Radford, Chris Moore, Gemma Clark, Mathew Diggle, Jess Tyson, Jamie Twycross, R Elizabeth Sockett
In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as "living antibiotics" to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28812884/controlling-bdellovibrio-bacteriovorus-gene-expression-and-predation-using-synthetic-riboswitches
#4
Mohammed Dwidar, Yohei Yokobayashi
Bdellovibrio bacteriovorus is a predatory bacterium that feeds on Gram-negative bacteria including a wide range of pathogens and thus has potential applications as a biocontrol agent. Owing to its unique life cycle, however, there are limited tools that enable genetic manipulation of B. bacteriovorus. This work describes our first steps toward engineering the predatory bacterium for practical applications by developing basic genetic parts to control gene expression. Specifically, we evaluated four robust promoters that are active during the attack phase of B...
August 18, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28725056/serum-albumin-and-osmolality-inhibit-bdellovibrio-bacteriovorus-predation-in-human-serum
#5
Hansol Im, Sangmo Son, Robert J Mitchell, Cheol-Min Ghim
We evaluated the bactericidal activity of Bdellovibrio bacteriovorus, strain HD100, within blood sera against bacterial strains commonly associated with bacteremic infections, including E. coli, Klebsiella pneumoniae and Salmonella enterica. Tests show that B. bacteriovorus HD100 is not susceptible to serum complement or its bactericidal activity. After a two hour exposure to human sera, the prey populations decreased 15- to 7,300-fold due to the serum complement activity while, in contrast, the B. bacteriovorus HD100 population showed a loss of only 33%...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710792/biophysical-investigation-of-type-a-putas-reveals-a-conserved-core-oligomeric-structure
#6
David A Korasick, Harkewal Singh, Travis A Pemberton, Min Luo, Richa Dhatwalia, John J Tanner
Many enzymes form homooligomers, yet the functional significance of self-association is seldom obvious. Herein, we examine the connection between oligomerization and catalytic function for proline utilization A (PutA) enzymes. PutAs are bifunctional enzymes that catalyze both reactions of proline catabolism. Type A PutAs are the smallest members of the family, possessing a minimal domain architecture consisting of N-terminal proline dehydrogenase and C-terminal l-glutamate-γ-semialdehyde dehydrogenase modules...
September 2017: FEBS Journal
https://www.readbyqxmd.com/read/28601973/attack-phase-bdellovibrio-bacteriovorus-responses-to-extracellular-nutrients-are-analogous-to-those-seen-during-late-intraperiplasmic-growth
#7
Mohammed Dwidar, Hansol Im, Jeong Kon Seo, Robert J Mitchell
Bdellovibrio bacteriovorus is a predatory bacterium which lives by invading the periplasm of gram-negative bacteria and consuming them from within. This predator was thought to be dependent upon prey for nutrients since it lacks genes encoding for critical enzymes involved in amino acid biosynthesis. This study, however, found that planktonic attack-phase predators are not just dependent upon prey for nutrients, but rather, they respond to nutrients in the surrounding medium and, subsequently, synthesize and secrete proteases in a nutrient-dependent manner...
June 10, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28510364/lyophilization-of-bdellovibrio-bacteriovorus-109j-for-long-term-storage
#8
Mélanie J Boileau, Rinosh Mani, Kenneth D Clinkenbeard
Bdellovibrio bacteriovorus 109J is a Gram-negative predatory bacterium with obligate host dependency on other Gram-negative bacteria. This bacteriolytic predator collides with, enters, and establishes growth within the prey (host) periplasm, eventually lysing the prey cell wall to release fresh, motile B. bacteriovorus progeny. Laboratory maintenance of B. bacteriovorus has been previously described by other investigators. The protocols included in this unit deal with the technique required to lyophilize or freeze dry host-dependent B...
May 16, 2017: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/28500337/examining-the-efficacy-of-intravenous-administration-of-predatory-bacteria-in-rats
#9
Kenneth Shatzkes, Eric Singleton, Chi Tang, Michael Zuena, Sean Shukla, Shilpi Gupta, Sonal Dharani, Joseph Rinaggio, Daniel E Kadouri, Nancy D Connell
The proteobacteria Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are obligate predators of Gram-negative bacteria, and have been proposed to be used to treat multidrug-resistant bacterial infections. The ability of predatory bacteria to reduce bacterial burden in vivo within the lungs of rats has been demonstrated, but it was unknown if predatory bacteria can attenuate systemic bacterial burden administered intravenously. In this study, we first assessed the safety of intravenous inoculation of predatory bacteria in rats...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432347/identification-and-characterization-of-differentially-regulated-type-ivb-pilin-genes-necessary-for-predation-in-obligate-bacterial-predators
#10
Ofir Avidan, Margarita Petrenko, René Becker, Sebastian Beck, Michael Linscheid, Shmuel Pietrokovski, Edouard Jurkevitch
Bdellovibrio bacteriovorus is an obligate predator of bacteria that grows and divides within the periplasm of its prey. Functions involved in the early steps of predation have been identified and characterized, but mediators of prey invasion are still poorly detailed. By combining omics data available for Bdellovibrio and like organisms (BALO's), we identified 43 genes expressed in B. bacteriovorus during the early interaction with prey. These included genes in a tight adherence (TAD) operon encoding for two type IVb fimbriae-like pilin proteins (flp1 and flp2), and their processing and export machinery...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28367880/predation-efficacy-of-bdellovibrio-bacteriovorus-on-multidrug-resistant-clinical-pathogens-and-their-corresponding-biofilms
#11
Yao Sun, Jianzhong Ye, Yuanbo Hou, Huale Chen, Jianming Cao, Tieli Zhou
The aim of the present study was to evaluate the predation efficacy of Bdellovibrio bacteriovorus on multidrug-resistant (MDR) or extensive drug resistant (XDR) gram-negative pathogens and their corresponding biofilms. In this study, we examined the ability of B. bacteriovorus to prey on MDR and XDR gram-negative clinical bacteria, including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii. Results showed that B. bacteriovorus was able to prey on all planktonic cultures, among which the most efficient predation was found for drug-resistant E...
March 28, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28355554/hydrodynamic-hunters
#12
Hossein Jashnsaz, Mohammed Al Juboori, Corey Weistuch, Nicholas Miller, Tyler Nguyen, Viktoria Meyerhoff, Bryan McCoy, Stephanie Perkins, Ross Wallgren, Bruce D Ray, Konstantinos Tsekouras, Gregory G Anderson, Steve Pressé
The Gram-negative Bdellovibrio bacteriovorus (BV) is a model bacterial predator that hunts other bacteria and may serve as a living antibiotic. Despite over 50 years since its discovery, it is suggested that BV probably collides into its prey at random. It remains unclear to what degree, if any, BV uses chemical cues to target its prey. The targeted search problem by the predator for its prey in three dimensions is a difficult problem: it requires the predator to sensitively detect prey and forecast its mobile prey's future position on the basis of previously detected signal...
March 28, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28334102/predation-by-bdellovibrio-bacteriovorus-significantly-reduces-viability-and-alters-the-microbial-community-composition-of-activated-sludge-flocs-and-granules
#13
Shugeng Feng, Chuan Hao Tan, Florentin Constancias, Gurjeet S Kohli, Yehuda Cohen, Scott A Rice
We recently isolated and characterised a predatory Bdellovibrio bacteriovorus strain from activated sludge (Ulu Pandan Water Reclamation Plant, Singapore), and this strain, B. bacteriovorus UP, was able to prey upon a broad spectrum of bacterial isolates from the activated sludge when grown as planktonic cells or as biofilms. Here, we have tested the effect of Bdellovibrio predation on floccular and granular sludge to determine if the spatial organisation, loosely or tightly aggregated communities, was protective from predation...
April 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28262674/effect-of-predatory-bacteria-on-the-gut-bacterial-microbiota-in-rats
#14
Kenneth Shatzkes, Chi Tang, Eric Singleton, Sean Shukla, Michael Zuena, Shilpi Gupta, Sonal Dharani, Joseph Rinaggio, Nancy D Connell, Daniel E Kadouri
Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are Gram-negative proteobacteria that are obligate predators of other Gram-negative bacteria and are considered potential alternatives to antibiotics. Most studies focusing on predatory bacteria have been performed in vitro, thus the effect of predatory bacteria on a live host, including the impact on the ecology of the native microbiota, has yet to be fully examined. In this study, intrarectal inoculations of Sprague-Dawley rats with predatory bacteria were performed...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28177793/biocontrol-of-burkholderia-cepacia-complex-bacteria-and-bacterial-phytopathogens-by-bdellovibrio-bacteriovorus
#15
Damian McNeely, Ryan M Chanyi, James S Dooley, John E Moore, Susan F Koval
Bdellovibrio and like organisms are predatory bacteria that have the unusual property of using the cytoplasmic constituents of other Gram-negative bacteria as nutrients. These predators may thus provide an alternative approach to the biocontrol of human and plant pathogens. Predators were isolated on Burkholderia cenocepacia K56-2 and J2315 as prey cells, in enrichment cultures with soil and sewage. Three isolates (DM7C, DM8A, and DM11A) were identified as Bdellovibrio bacteriovorus on the basis of morphology, a periplasmic life cycle, and 16S rRNA gene sequencing...
April 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28053112/modelling-and-parameter-inference-of-predator-prey-dynamics-in-heterogeneous-environments-using-the-direct-integral-approach
#16
Itai Dattner, Ezer Miller, Margarita Petrenko, Daniel E Kadouri, Edouard Jurkevitch, Amit Huppert
Most bacterial habitats are topographically complex in the micro scale. Important examples include the gastrointestinal and tracheal tracts, and the soil. Although there are myriad theoretical studies that explore the role of spatial structures on antagonistic interactions (predation, competition) among animals, there are many fewer experimental studies that have explored, validated and quantified their predictions. In this study, we experimentally monitored the temporal dynamic of the predatory bacterium Bdellovibrio bacteriovorus, and its prey, the bacterium Burkholderia stabilis in a structured habitat consisting of sand under various regimes of wetness...
January 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/27965633/initiation-of-chromosomal-replication-in-predatory-bacterium-bdellovibrio-bacteriovorus
#17
Łukasz Makowski, Rafał Donczew, Christoph Weigel, Anna Zawilak-Pawlik, Jolanta Zakrzewska-Czerwińska
Bdellovibrio bacteriovorus is a small Gram-negative predatory bacterium that attacks other Gram-negative bacteria, including many animal, human, and plant pathogens. This bacterium exhibits a peculiar biphasic life cycle during which two different types of cells are produced: non-replicating highly motile cells (the free-living phase) and replicating cells (the intracellular-growth phase). The process of chromosomal replication in B. bacteriovorus must therefore be temporally and spatially regulated to ensure that it is coordinated with cell differentiation and cell cycle progression...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27940543/bspk-a-serine-protease-from-the-predatory-bacterium-bdellovibrio-bacteriovorus-with-utility-for-analysis-of-therapeutic-antibodies
#18
Eleni Bratanis, Henrik Molina, Andreas Naegeli, Mattias Collin, Rolf Lood
The development of therapeutic and diagnostic antibodies is a rapidly growing field of research, being the fastest expanding group of products on the pharmaceutical market, and appropriate quality controls are crucial for their application. We have identified and characterized the serine protease termed BspK (Bdellovibrio serine protease K) from Bdellovibrio bacteriovorus and here show its activity on antibodies. Mutation of the serine residue at position 230 rendered the protease inactive. Further investigations of BspK enzymatic characteristics revealed autoproteolytic activity, resulting in numerous cleavage products...
February 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27915281/effects-of-predation-and-dispersal-on-bacterial-abundance-and-contaminant-biodegradation
#19
Sally Otto, Hauke Harms, Lukas Y Wick
Research into the biodegradation of soil contaminants has rarely addressed the consequences of predator-prey interactions. Here, we investigated the joint effect of predation and dispersal networks on contaminant degradation by linking spatial abundances of degrader (Pseudomonas fluorescens LP6a) and predator (Bdellovibrio bacteriovorus) bacteria to the degradation of the major soil contaminant phenanthrene (PHE). We used a laboratory microcosm with a PHE passive dosing system and a glass fiber network to facilitate bacterial dispersal...
February 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27889262/injections-of-predatory-bacteria-work-alongside-host-immune-cells-to-treat-shigella-infection-in-zebrafish-larvae
#20
Alexandra R Willis, Christopher Moore, Maria Mazon-Moya, Sina Krokowski, Carey Lambert, Robert Till, Serge Mostowy, R Elizabeth Sockett
Bdellovibrio bacteriovorus are predatory bacteria that invade and kill a range of Gram-negative bacterial pathogens in natural environments and in vitro [1, 2]. In this study, we investigated Bdellovibrio as an injected, antibacterial treatment in vivo, using zebrafish (Danio rerio) larvae infected with an antibiotic-resistant strain of the human pathogen Shigella flexneri. When injected alone, Bdellovibrio can persist for more than 24 hr in vivo yet exert no pathogenic effects on zebrafish larvae. Bdellovibrio injection of zebrafish containing a lethal dose of Shigella promotes pathogen killing, leading to increased zebrafish survival...
December 19, 2016: Current Biology: CB
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