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Plant propagation

Delphine Mieulet, Sylvie Jolivet, Maud Rivard, Laurence Cromer, Aurore Vernet, Pauline Mayonove, Lucie Pereira, Gaëtan Droc, Brigitte Courtois, Emmanuel Guiderdoni, Raphael Mercier
Introduction of clonal reproduction through seeds (apomixis) in crops has the potential to revolutionize agriculture by allowing self-propagation of any elite variety, in particular F1 hybrids. In the sexual model plant Arabidopsis thaliana synthetic clonal reproduction through seeds can be artificially implemented by (i) combining three mutations to turn meiosis into mitosis (MiMe) and (ii) crossing the obtained clonal gametes with a line expressing modified CENH3 and whose genome is eliminated in the zygote...
October 21, 2016: Cell Research
Jaafar Juju Nakasha, Uma Rani Sinniah, Nurashikin Kemat, Kumara Swamy Mallappa
BACKGROUND: Chlorophytum borivilianum is an industrially valued medicinal crop. Propagation through seeds is not feasible because of low germination percentage and long dormancy period. Therefore, callus culture and plant regeneration can be an alternative to improve this crop production. Also, callus can serve as an alternative source of bioactive compounds. OBJECTIVE: To evaluate the effect of different phytohormones on callus induction, subculture cycle, and regeneration studies of callus in C...
July 2016: Pharmacognosy Magazine
Jinping Zhao, Xian Zhang, Yiguo Hong, Yule Liu
In plants, the chloroplast is the organelle that conducts photosynthesis. It has been known that chloroplast is involved in virus infection of plants for approximate 70 years. Recently, the subject of chloroplast-virus interplay is getting more and more attention. In this article we discuss the different aspects of chloroplast-virus interaction into three sections: the effect of virus infection on the structure and function of chloroplast, the role of chloroplast in virus infection cycle, and the function of chloroplast in host defense against viruses...
2016: Frontiers in Microbiology
Luis Abdala-Roberts, Johnattan Hernández-Cumplido, Luis Chel-Guerrero, David Betancur-Ancona, Betty Benrey, Xoaquín Moreira
PREMISE OF STUDY: Although there is increasing recognition of the effects of plant intraspecific diversity on consumers, the mechanisms by which such effects cascade-up to higher trophic levels remain elusive. METHODS: We evaluated the effects of plant (lima bean, Phaseolus lunatus) intraspecific diversity on a suite of insect herbivores (leaf-chewers, aphids, and seed-eating beetles) and their third trophic-level associates (parasitoids and aphid-tending ants)...
October 18, 2016: American Journal of Botany
Alexander G Volkov, Eunice K Nyasani, Clayton Tuckett, Jessenia M Scott, Mariah M Z Jackson, Esther A Greeman, Ariane S Greenidge, Devin O Cohen, Maia I Volkova, Yuri B Shtessel
Electrostimulation of plants can induce plant movements, activation of ion channels, ion transport, gene expression, enzymatic systems activation, electrical signaling, plant-cell damage, enhanced wound healing, and influence plant growth. Here we found that electrical networks in plant tissues have electrical differentiators. The amplitude of electrical responses decreases along a leaf and increases by decreasing the distance between polarizing Pt-electrodes. Intercellular Ag/AgCl electrodes inserted in a leaf and extracellular Ag/AgCl electrodes attached to the leaf surface were used to detect the electrotonic potential propagation along a leaf of Aloe vera...
October 12, 2016: Bioelectrochemistry
Ponnusamy Baskaran, Aloka Kumari, Bhekumthetho Ncube, Johannes Van Staden
Mondia whitei (Hook.f.) Skeels is an important endangered medicinal and commercial plant in South Africa. In vitro propagation systems are required for biomass production and bioactivity analysis to supplement wild resources/stocks. Adventitious roots from somatic embryogenic explants using suspension culture and ex vitro-grown plants produced via somatic embryogenesis were established using different plant growth regulator treatments. The adventitious root biomass and different parts of ex vitro-grown and mother plants were used to investigate the potential for acetylcholinesterase (AChE) and antibacterial activities...
2016: Frontiers in Pharmacology
Carla Marrassini, Claudia Anesini
There is a well known link between inflammation and cancer during initiation, propagation and metastasis. Urera aurantiaca (UA) Wedd. (Urticaceae) is a medicinal plant used in traditional medicine to treat inflammatory processes with proven in vivo antiinflammatory and antinociceptive effects. The effects of a methanolic extract (UA) and a purified fraction (PF) on the proliferation of normal and tumoral lymphocytes under the effect of prostaglandin E2 (PGE2 ) and on nitric oxide production by lipopolysaccharide-stimulated macrophages was evaluated...
October 17, 2016: Phytotherapy Research: PTR
Agnieszka Ilczuk, Ewelina Jacygrad
Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N(6)-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA)...
2016: In Vitro Cellular & Developmental Biology. Plant: Journal of the Tissue Culture Association
Christiane Gebhardt
This article evaluates the main contributions of tomato, tobacco, petunia, potato, pepper and eggplant to classical and molecular plant genetics and genomics since the beginning of the twentieth century. Species from the Solanaceae family form integral parts of human civilizations as food sources and drugs since thousands of years, and, more recently, as ornamentals. Some Solanaceous species were subjects of classical and molecular genetic research over the last 100 years. The tomato was one of the principal models in twentieth century classical genetics and a pacemaker of genome analysis in plants including molecular linkage maps, positional cloning of disease resistance genes and quantitative trait loci (QTL)...
October 15, 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Melissa Yit Yee Kam, Li Chin Chai, Chiew Foan Chin
Aponogeton ulvaceus Baker (Aponogetonaceae) is a commercially important ornamental aquatic plant species with traditional medicinal uses. Due to the low survival rate of seedlings, propagation by conventional means has been met with many difficulties. In this study, botanical aspects of A. ulvaceus were examined with regards to the morphology, anatomy and physiology of the plant and an efficient protocol for its in vitro propagation using immature tuber explants has been established. The existence of glandular trichomes on the leaves was discovered and the occurrence of circumnutation in A...
2016: SpringerPlus
Walid Q Alali, Charles L Hofacre
Preharvest food safety in broiler production is a systematic approach to control the introduction, propagation, and dissemination of Salmonella and Campylobacter from breeder flocks to the end of their progeny (broilers) life cycle. New and revised more stringent performance standards for these pathogens at the processing plant level require continuous evaluation of the preharvest management practices and intervention strategies used by the poultry industry. The implementation of stricter biosecurity plans, vaccination of breeder flocks for Salmonella, and usage of feed that is free of animal by-products are some of the measures recommended to control the pathogens...
August 2016: Microbiology Spectrum
Siegfried Zerche, Klaus-Thomas Haensch, Uwe Druege, Mohammad-Reza Hajirezaei
BACKGROUND: Adventitious root (AR) formation in axillary shoot tip cuttings is a crucial physiological process for ornamental propagation that is utilised in global production chains for young plants. In this process, the nitrogen and carbohydrate metabolisms of a cutting are regulated by its total nitrogen content (Nt), dark exposure during transport and irradiance levels at distinct production sites and phases through a specific plasticity to readjust metabolite pools. Here, we examined how elevated Nt contents with a combined dark exposure of cuttings influence their internal N-pools including free amino acids and considered early anatomic events of AR formation as well as further root development in Petunia hybrida cuttings...
October 10, 2016: BMC Plant Biology
Qian Chen, Taiyun Wei
Insect-borne propagative plant viruses of agricultural importance are transmitted by sap-sucking insects. Although the infection routes of these viruses within the bodies of insect vectors are well established, cellular receptors on the microvilli, intercellular junctions, and basal lamina for mediating viral entry or spread in insect gut epithelium have not been well identified or characterized. Recent trends in the field are opening questions on how viruses exploit actin-based tubule motility to overcome insect gut epithelium barriers after viral entry in epithelium...
August 2016: Current Opinion in Insect Science
Mohaddese Mahboubi
ETHNOPHARMACOLOGICAL RELEVANCE: In Iranian Traditional Medicine, M. communis is a famous plant in treatment of oral ulcers and "Gholaa"- the ancient name of aphthous. OBJECTIVE: The aim of this review is to create a bridge between the traditional claims about the application of M. communis in treatment of "Gholaa" and its prescription for aphthous, the current form of "Gholaa" in modern medicine. METHODS: We extracted the information about the application of M...
September 29, 2016: Journal of Ethnopharmacology
H J Park, W-Y Kim, J M Pardo, D-J Yun
Plants have adapted to environmental changes and stresses over generations. The decision of transition from the vegetative to reproductive stage is critical, particularly under unfavorable conditions. Thus, plants appear to have developed mechanisms by which environmental factors or inputs are transmitted to stress response signaling pathways to confer tolerance and are simultaneously integrated into flowering regulation pathways (photoperiod, vernalization, autonomous, and gibberellic acid signaling) to propagate the next generation...
2016: International Review of Cell and Molecular Biology
Rosemarije Buitenhuis, Michael Brownbridge, Angela Brommit, Taro Saito, Graeme Murphy
(1) Global movement of propagative plant material is a major pathway for introduction of Bemisia tabaci (Hemiptera: Aleyrodidae) into poinsettia greenhouses. Starting a poinsettia crop with high pest numbers disrupts otherwise successful biological control programs and widespread resistance of B. tabaci against pesticides is limiting growers' options to control this pest; (2) This study investigated the use of several biopesticides (mineral oil, insecticidal soap, Beauveria bassiana, Isaria fumosorosea, Steinernema feltiae) and combinations of these products as immersion treatments (cutting dips) to control B...
2016: Insects
Chad E Niederhuth, Adam J Bewick, Lexiang Ji, Magdy S Alabady, Kyung Do Kim, Qing Li, Nicholas A Rohr, Aditi Rambani, John M Burke, Joshua A Udall, Chiedozie Egesi, Jeremy Schmutz, Jane Grimwood, Scott A Jackson, Nathan M Springer, Robert J Schmitz
BACKGROUND: DNA methylation is an important feature of plant epigenomes, involved in the formation of heterochromatin and affecting gene expression. Extensive variation of DNA methylation patterns within a species has been uncovered from studies of natural variation. However, the extent to which DNA methylation varies between flowering plant species is still unclear. To understand the variation in genomic patterning of DNA methylation across flowering plant species, we compared single base resolution DNA methylomes of 34 diverse angiosperm species...
September 27, 2016: Genome Biology
Pablo López-Soriano, Karine Boyer, Sophie Cesbron, María Clara Morente, Javier Peñalver, Ana Palacio-Bielsa, Christian Vernière, María M López, Olivier Pruvost
Xanthomonas arboricola pv. pruni is the causal agent of the bacterial spot disease of stone fruits, almond and some ornamental Prunus species. In Spain it was first detected in 2002 and since then, several outbreaks have occurred in different regions affecting mainly Japanese plum, peach and almond, both in commercial orchards and nurseries. As the origin of the introduction(s) was unknown, we have assessed the genetic diversity of 239 X. arboricola pv. pruni strains collected from 11 Spanish provinces from 2002 to 2013 and 25 reference strains from international collections...
2016: PloS One
Anita Kumari, Dharam Singh, Sanjay Kumar
Podophyllotoxin is an aryltetralin lignan synthesized in several plant species, which is used in chemotherapies for cancers and tumor treatment. More potent semisynthetic derivatives of podophyllotoxin such as etoposide and teniposide are being developed and evaluated for their efficacy. To meet the ever increasing pharmaceutical needs, species having podophyllotoxin are uprooted extensively leading to the endangered status of selective species mainly Sinopodophyllum hexandrum. This has necessitated bioprospection of podophyllotoxin from different plant species to escalate the strain on this endangered species...
September 20, 2016: Critical Reviews in Biotechnology
Edith Kuprian, Tan D Tuong, Kristian Pfaller, Johanna Wagner, David P Livingston, Gilbert Neuner
Extracellular ice nucleation usually occurs at mild subzero temperatures in most plants. For persistent supercooling of certain plant parts ice barriers are necessary to prevent the entry of ice from already frozen tissues. The reproductive shoot of Calluna vulgaris is able to supercool down to below -22°C throughout all developmental stages (shoot elongation, flowering, fruiting) despite an established xylem conductivity. After localization of the persistent ice barrier between the reproductive and vegetative shoot at the base of the pedicel by infrared differential thermal analysis, the currently unknown structural features of the ice barrier tissue were anatomically analyzed on cross and longitudinal sections...
2016: PloS One
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