keyword
MENU ▼
Read by QxMD icon Read
search

Dilution effect

keyword
https://www.readbyqxmd.com/read/28434154/protective-effects-of-cell-free-supernatant-and-live-lactic-acid-bacteria-isolated-from-thai-pigs-against-a-pandemic-strain-of-porcine-epidemic-diarrhea-virus
#1
Wandee Sirichokchatchawan, Gun Temeeyasen, Dachrit Nilubol, Nuvee Prapasarakul
Porcine epidemic diarrhea virus (PEDV) is a coronavirus which causes severe diarrhea and fatal dehydration in piglets. In general, probiotic supplements could enhance recovery and protect piglets against enteric pathogens. Seven local lactic acid bacteria (LAB), (Ent. faecium 79N and 40N, Lact. plantarum 22F, 25F and 31F, Ped. acidilactici 72N and Ped. pentosaceus 77F) from pig feces were well-characterized as high potential probiotics. Cell-free supernatants (CFS) and live LAB were evaluated for antiviral activities by co-incubation on Vero cells and challenged with a pandemic strain of PEDV isolated from pigs in Thailand...
April 22, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28434115/interaction-of-selected-phenylpropenes-with-dipalmitoylphosphatidylcholine-membrane-and-their-relevance-to-antibacterial-activity
#2
Riham Gharib, Amal Najjar, Lizette Auezova, Catherine Charcosset, Hélène Greige-Gerges
The effect of structurally closely related phenylpropenes (PPs), estragole, anethole, eugenol, and isoeugenol, on the fluidity of dipalmitoyl phosphatidyl choline (DPPC) liposome membrane was investigated by DSC, Raman, and fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH). Liposomes were prepared by thin-film hydration method at various DPPC:PP molar ratios. The DPH anisotropy measurements of blank and PP-loaded liposomes were performed at 28, 41, and 50 °C, which correspond, respectively, to gel phase, main transition temperature of DPPC, and liquid phase...
April 22, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28433920/proteomic-analysis-of-sockeye-salmon-serum-as-a-tool-for-biomarker-discovery-and-new-insight-into-the-sublethal-toxicity-of-diluted-bitumen
#3
Sarah L Alderman, Laura A Dindia, Christopher J Kennedy, Anthony P Farrell, Todd E Gillis
Pipelines carrying diluted bitumen (dilbit) from Canada's oil sands traverse North America, including the freshwater habitat of Pacific salmon, posing a risk of environmental release and aquatic exposure. Swimming performance is impacted in juvenile sockeye (Oncorhynchus nerka) exposed to dilbit; therefore biomarkers of dilbit exposure will be valuable for monitoring at-risk salmon stocks. This study characterized changes in the serum proteome of sockeye exposed to a sub-lethal and environmentally relevant concentration of dilbit using isobaric tags for relative and absolute quantitation (iTRAQ), and included a range of experimental conditions to permit identification of biomarkers that are robust across time (1 and 4wk) and exercise level (at rest and following a swim test)...
April 12, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28433743/the-antibiotic-polymyxin-b-exhibits-novel-antifungal-activity-against-fusarium-species
#4
Li-Hang Hsu, Hsuan-Fu Wang, Pei-Lun Sun, Fung-Rong Hu, Ying-Lien Chen
The genus Fusarium comprises many species, including Fusarium oxysporum, Fusarium solani, Fusarium graminearum and Fusarium verticillioides, and causes severe infections in plants and humans. In clinical settings, Fusarium is the third most frequent mould to cause invasive fungal infections after Aspergillus and the Mucorales. Fusarium solani and F. oxysporum are the most prevalent Fusarium spp. causing clinical disease. However, few effective antifungal drugs are available to treat human and plant Fusarium infections...
April 19, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28433142/enhanced-degradation-of-chitosan-by-applying-plasma-treatment-in-combination-with-oxidizing-agents-for-potential-use-as-an-anticancer-agent
#5
Chayanaphat Chokradjaroen, Ratana Rujiravanit, Anyarat Watthanaphanit, Sewan Theeramunkong, Nagahiro Saito, Kazuko Yamashita, Ryuichi Arakawa
Solution plasma (SP) treatment in combination with oxidizing agents, i.e., hydrogen peroxide (H2O2), potassium persulfate (K2S2O8) and sodium nitrite (NaNO2) were adopted to chitosan degradation in order to achieve fast degradation rate, low chemicals used and high yield of low-molecular-weight chitosan and chitooligosaccharide (COS). Among the studied oxidizing agents, H2O2 was found to be the best choice in terms of appreciable molecular weight reduction without major change in chemical structure of the degraded products of chitosan...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28433057/low-cost-humic-acid-bonded-silica-as-an-effective-solid-phase-extraction-sorbent-for-convenient-determination-of-aflatoxins-in-edible-oils
#6
Neng-Zhi Zhou, Ping Liu, Xiao-Chuan Su, Yan-Hua Liao, Ning-Sheng Lei, Yong-Hong Liang, Shao-Huan Zhou, Wen-Si Lin, Jie Chen, Yu-Qi Feng, Yang Tang
Aflatoxins (AFs) are highly toxic, mutagenic, carcinogenic, and teratogenic secondary metabolites produced by the toxigenic fungi Aspergillus flavus and Aspergillus parasiticus. AFs tend to contaminate a wide range of foods which is a serious and recurring food safety problem worldwide. Currently, immunoaffinity chromatography (IAC) has become the most conventional sample clean-up method for determining AFs in foodstuffs. However, IAC method is limited in the large-scale food analysis because it requires the use of expensive disposable cartridges and the IA procedure is time-consuming...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28433055/pyrenyl-carbon-nanostructures-for-ultrasensitive-measurements-of-formaldehyde-in-urine
#7
Gayan Premaratne, Sabrina Farias, Sadagopan Krishnan
Measurement of ultra-low (e.g., parts-per-billion) levels of small-molecule markers in body fluids (e.g., serum, urine, saliva) involves a considerable challenge in view of designing assay strategies with sensitivity and selectivity. Herein we report for the first time an amperometric nano-bioelectrode design that uniquely combines 1-pyrenebutyric acid units pi-pi stacked with carboxylated multiwalled carbon nanotubes on the surface of gold screen printed electrodes for covalent attachment of NAD(+) dependent formaldehyde dehydrogenase (FDH)...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28432989/the-effect-of-natural-ventilation-strategy-on-indoor-air-quality-in-schools
#8
Luca Stabile, Marco Dell'Isola, Aldo Russi, Angelamaria Massimo, Giorgio Buonanno
In order to reduce children's exposure to pollutants in classrooms a proper ventilation strategy need to be adopted. Such strategy is even more important in naturally ventilated schools where the air exchange rate is only based on the manual airing of classrooms. The present work aimed to evaluate the effect of the manual airing strategy on indoor air quality in Italian classrooms. For this aim, schools located in the Central Italy were investigated. Indoor air quality was studied in terms of CO2, particle number and PM concentrations and compared to corresponding outdoor levels...
April 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28432941/validation-of-a-spe-hplc-uv-method-for-the-quantification-of-a-new-er-specific-photosensitizer-or-141-in-blood-serum-using-total-error-concept
#9
Emilie Bony, Yohan Mace, Adán Pinto, David Delvaux, Robert Kiss, Olivier Riant, Olivier Feron, Joëlle Quetin-Leclercq
The aim of this work is to develop the first validated HPLC-UV method quantification in blood serum for a new endoplasmic reticulum (ER)-specific benzophenazine photosensitizer (OR-141). A fast solid phase extraction (SPE) cleaning sample procedure was achieved on C18 encapped (ec) SPE cartridges and the separation was performed on a RP-18e column (5μM) using an isocratic elution with methanol. The method has been fully validated according to accuracy profiles based on total error and tolerance intervals. Calibration was performed in the matrix and trueness (<4...
April 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28431580/cripto-1-acts-as-a-functional-marker-of-cancer-stem-like-cells-and-predicts-prognosis-of-the-patients-in-esophageal-squamous-cell-carcinoma
#10
Qiang Liu, Xiang Cui, Xi Yu, Bai-Shi-Jiao Bian, Feng Qian, Xu-Gang Hu, Cheng-Dong Ji, Lang Yang, Yong Ren, Wei Cui, Xia Zhang, Peng Zhang, Ji Ming Wang, You-Hong Cui, Xiu-Wu Bian
BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is highly malignant with highly invasive and metastatic capabilities and poor prognosis. It is believed that the ESCC cancer stem-like cells (ECSLCs) are critical for tumorigenicity, invasion and metastasis of ESCC. However, the properties of ECSLCs vary with different markers used in isolation, so that new and more effective markers of ECSLCs need to be identified. This study aimed to estimate the potentiality of Cripto-1 (CR-1) as an ECSLC surface marker and investigate the clinical significance of CR-1 expression in ESCC...
April 21, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28431529/hirbin-high-resolution-identification-of-differentially-abundant-functions-in-metagenomes
#11
Tobias Österlund, Viktor Jonsson, Erik Kristiansson
BACKGROUND: Gene-centric analysis of metagenomics data provides information about the biochemical functions present in a microbiome under a certain condition. The ability to identify significant differences in functions between metagenomes is dependent on accurate classification and quantification of the sequence reads (binning). However, biological effects acting on specific functions may be overlooked if the classes are too general. METHODS: Here we introduce High-Resolution Binning (HirBin), a new method for gene-centric analysis of metagenomes...
April 21, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28431370/hydrodynamic-influence-on-reservoir-sustainability-in-semi-arid-climate-a-physicochemical-and-environmental-isotopic-study
#12
Rawaa Ammar, Véronique Kazpard, Antoine G El Samrani, Nabil Amacha, Zeinab Saad, Lei Chou
Water scarcity and increasing water demand require the development of water management plans such as establishing artificial lakes and dams. Plans to meet water needs are faced by uprising challenges to improve water quality and to ensure the sustainability of hydro-projects. Environmental isotopes coupled to water physicochemical characteristics were investigated over a biennial cycle to assess both geomorphological and environmental impacts on the water quality of a reservoir situated in an intensively used agricultural watershed under a Mediterranean semi-arid climate...
April 17, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28431360/contrasting-dual-c-cl-isotope-fractionation-offers-potential-to-distinguish-reductive-chloroethene-transformation-from-breakdown-by-permanganate
#13
Eylem Doğan-Subaşı, Martin Elsner, Shiran Qiu, Stefan Cretnik, Siavash Atashgahi, Orfan Shouakar-Stash, Nico Boon, Winnie Dejonghe, Leen Bastiaens
cis-1,2-Dichloroethene (cis-DCE) and trichloroethene (TCE) are persistent, toxic and mobile pollutants in groundwater systems. They are both conducive to reductive dehalogenation and to oxidation by permanganate. In this study, the potential of dual element (C, Cl) compound specific isotope analyses (CSIA) for distinguishing between chemical oxidation and anaerobic reductive dechlorination of cis-DCE and TCE was investigated. Well-controlled cis-DCE degradation batch tests gave similar carbon isotope enrichment factors εC (‰), but starkly contrasting dual element isotope slopes Δδ(13)C/Δδ(37)Cl for permanganate oxidation (εC=-26‰±6‰, Δδ(13)C/Δδ(37)Cl≈-125±47) compared to reductive dechlorination (εC=-18‰±4‰, Δδ(13)C/Δδ(37)Cl≈4...
April 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28431096/a-new-rapid-resazurin-based-microdilution-assay-for-antimicrobial-susceptibility-testing-of-neisseria-gonorrhoeae
#14
Sunniva Foerster, Valentino Desilvestro, Lucy J Hathaway, Christian L Althaus, Magnus Unemo
Objectives: Rapid, cost-effective and objective methods for antimicrobial susceptibility testing of Neisseria gonorrhoeae would greatly enhance surveillance of antimicrobial resistance. Etest, disc diffusion and agar dilution methods are subjective, mostly laborious for large-scale testing and take ∼24 h. We aimed to develop a rapid broth microdilution assay using resazurin (blue), which is converted into resorufin (pink fluorescence) in the presence of viable bacteria. Methods: The resazurin-based broth microdilution assay was established using 132 N...
April 19, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28430894/pooling-and-the-potential-dilution-of-repeat-influenza-vaccination-effects
#15
Danuta M Skowronski, Catharine Chambers
No abstract text is available yet for this article.
April 19, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28430448/structural-and-rheological-properties-of-temperature-responsive-amphiphilic-triblock-copolymers-in-aqueous-media
#16
Josefine Eilsø Nielsen, Kaizheng Zhu, Sverre Arne Sande, Lubomír Kováčik, Dušan Cmarko, Kenneth Dahl Knudsen, Bo Nyström
Thermoresponsive amphiphilic biodegradable block copolymers of the type poly(-caprolactone-co-lactide)-poly(ethylene glycol)-poly(-caprolactone-co-lactide) (PCLA-PEGm-PCLA) have great potential for various biomedical applications. In the present study, we have surveyed the effects of PEG-spacer length (m=1000 and 1500), temperature, and polymer concentration on the self-assembling process to form supramolecular structures in aqueous solutions of the PCLA-PEGm-PCLA copolymer. This copolymer has a lower critical solution temperature, and the cloud point depends on both concentration and PEG-length...
April 21, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28430315/adding-access-blood-flow-surveillance-reduces-thrombosis-and-improves-arteriovenous-fistula-patency-a-randomized-controlled-trial
#17
Inés Aragoncillo, Soraya Abad, Silvia Caldés, Yésika Amézquita, Almudena Vega, Antonio Cirugeda, Cristina Moratilla, José Ibeas, Ramón Roca-Tey, Cristina Fernández, Nicolás Macías, Borja Quiroga, Ana Blanco, Maite Villaverde, Caridad Ruiz, Belén Martín, Asunción M Ruiz, Jara Ampuero, Fernando de Alvaro, Juan M López-Gómez
PURPOSE: Stenosis is the main cause of arteriovenous fistula (AVF) failure. It is still unclear whether surveillance based on vascular access blood flow (QA) enhances AVF function and longevity. METHODS: We conducted a three-year follow-up randomized, controlled, multicenter, open-label trial to compare QA-based surveillance and pre-emptive repair of subclinical stenosis with standard monitoring/surveillance techniques in prevalent mature AVFs. AVFs were randomized to either the control group (surveillance based on classic alarm criteria; n = 104) or to the QA group (QA measured quarterly using Doppler ultrasound [M-Turbo®] and ultrasound dilution [Transonic®] added to classic surveillance; n = 103)...
April 20, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/28429943/on-the-size-and-origins-of-long-range-orientational-water-correlations-in-dilute-aqueous-salt-solutions
#18
Eva Pluharova, Damien Laage, Pavel Jungwirth
Long-range ordering of water around solutes has been invoked repeatedly in the literature as the key to understanding its biological function. Recently, it has been shown that in a dilute 8 mM aqueous NaCl solution the orientational correlation between water molecules extends beyond 8 nanometers. This was interpreted as arising from a long-range effect induced by the ions on the water collective hydrogen-bond interactions. Within this interpretation, each ion was supposed to affect tens of thousands or more water molecules in its surroundings, invoking a picture of ordered domains extending to several nanometers...
April 21, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28428740/protein-quality-control-by-molecular-chaperones-in-neurodegeneration
#19
REVIEW
Aaron Ciechanover, Yong Tae Kwon
Protein homeostasis (proteostasis) requires the timely degradation of misfolded proteins and their aggregates by protein quality control (PQC), of which molecular chaperones are an essential component. Compared with other cell types, PQC in neurons is particularly challenging because they have a unique cellular structure with long extensions. Making it worse, neurons are postmitotic, i.e., cannot dilute toxic substances by division, and, thus, are highly sensitive to misfolded proteins, especially as they age...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28427918/endocan-is-a-stable-circulating-molecule-in-icu-patients
#20
Gaudet Alexandre, Chenevier-Gobeaux Camille, Parmentier Erika, Delobel Jean-Edouard, Dubucquoi Sylvain, Mathieu Daniel, Lassalle Philippe, De Freitas Caires Nathalie
BACKGROUND: Endocan is a lung endothelial cell secreted proteoglycan, possessing multiple physiological roles and potential therapeutic and diagnostic utility as biomarker in pneumonia and acute respiratory distress syndrome. Endocan synthesis and secretion can be induced by proinflammatory cytokines such as TNF-α, but can also be subject of proteolytic degradation causing preanalytical variation. METHODS: We investigated the stability of endocan in conventional serum, plasma, anticoagulated whole blood, as well as whole blood and plasma stabilized with protease inhibitors...
April 17, 2017: Clinical Biochemistry
keyword
keyword
87988
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"