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https://www.readbyqxmd.com/read/28822347/screening-and-confirmation-of-steroids-and-nitroimidazoles-in-urine-blood-and-food-matrices-sample-preparation-methods-and-liquid-chromatography-tandem-mass-spectrometric-separations
#1
Ádám Tölgyesi, Enikő Barta, Andrea Simon, Thomas J McDonald, Virender K Sharma
Veterinary drugs containing synthetic anabolic steroid and nitroimidazole active agents are not allowed for their applications in livestock of the European Union (EU). This paper presents analyses of twelve selected steroids and six nitroimidazole antibiotics at low levels (1.56μg/L-4.95μg/L and 0.17μg/kg-2.14μg/kg, respectively) in body fluids and egg incurred samples. Analyses involved clean-up procedures, high performance liquid chromatography (HPLC) separation, and tandem mass spectrometric screening and confirmatory methods...
August 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28822306/xyloglucan-endotransglucosylase-hydrolases-xths-are-inactivated-by-binding-to-glass-and-cellulosic-surfaces-and-released-in-active-form-by-a-heat-stable-polymer-from-cauliflower-florets
#2
Sandra C Sharples, Tu C Nguyen-Phan, Stephen C Fry
Xyloglucan endotransglucosylase (XET) activity, which cuts and re-joins hemicellulose chains in the plant cell wall, contributing to wall assembly and growth regulation, is the major activity of XTH proteins. During purification, XTHs often lose XET activity which, however, is restored by treatment with certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets. It was not known whether the XTH-activating factor (XAF) present in CHPs works by promoting (e.g. allosterically) XET activity or by re-solubilising sequestered XTH proteins...
August 5, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28822159/-effect-of-sodium-houttuyfonate-in-combination-with-erythromycin-on-luxs-agr-rna%C3%A2-of-staphylococus-epidermidis
#3
Gan-Fei Xu, Jing-Jing Wang, Da-Qiang Wu, Yan Guan
Quorum sensing of bacteria and its specific gene expression regulation have a very important role in bacterial biofilm formation. LuxS and agr are the key regulatory genes in quorum sensing of Staphylococcus epidermidis, and RNA Ⅲ is the effector molecule of agr system. In order to evaluate the effects of sodium houttuyfonate in combination with erythromycin on the transcription level of S. epidermidis, serial dilution method was used to determine the MIC of sodium houttuyfonate, erythromycin and vancomycin on S...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28821002/modeling-the-fate-and-transport-of-17%C3%AE-estradiol-in-the-south-river-watershed-in-virginia
#4
Xiaomin Zhao, Wu-Seng Lung
Hormones excreted by livestock metabolisms often enter surface water through feces and urine and can potentially cause adverse impacts to aquatic biota. This study involved a modeling analysis of 17β-estradiol (E2), a prevalent estrogen, in the South River watershed located in Augusta County, Virginia from 2013 to 2015. Cattle manure, poultry litter, biosolids, septic systems, and wastewater treatment plants (WWTPs) were considered as sources of E2 in this study. The EPA's BASINS modeling framework was configured to track the fate and transport of E2...
August 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28820488/phase-behaviour-and-miscibility-studies-of-collagen-silk-fibroin-macromolecular-system-in-dilute-solutions-and-solid-state
#5
Ima Ghaeli, Mariana A de Moraes, Marisa M Beppu, Katarzyna Lewandowska, Alina Sionkowska, Frederico Ferreira-da-Silva, Maria P Ferraz, Fernando J Monteiro
Miscibility is an important issue in biopolymer blends for analysis of the behavior of polymer pairs through the detection of phase separation and improvement of the mechanical and physical properties of the blend. This study presents the formulation of a stable and one-phase mixture of collagen and regenerated silk fibroin (RSF), with the highest miscibility ratio between these two macromolecules, through inducing electrostatic interactions, using salt ions. For this aim, a ternary phase diagram was experimentally built for the mixtures, based on observations of phase behavior of blend solutions with various ratios...
August 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28816077/in-vitro-evaluation-of-the-occlusive-properties-of-the-artventive-eos-occlusion-device-for-transrenal-ureteral-occlusion
#6
Daniel Kuetting, Carsten Meyer, Hans Heinz Schild, Claus Christian Pieper
PURPOSE: Current techniques for percutaneous ureteral occlusion are either technically difficult or not satisfactory due to frequent ureteral recanalization. The purpose of this in vitro study was to evaluate the occlusive properties of an "off the shelf" solution (EOS; ArtVentive Medical Group, Inc, Carlsbad, CA/USA) for transrenal ureteral occlusion. MATERIALS AND METHODS: 8 and 11 mm ePTFE covered ArtVentive EOS devices were used in 10 porcine models. Experiments were performed in explanted porcine ureters to simulate physiologic conditions...
August 17, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28815637/synergistic-antibacterial-and-antibiofilm-efficacy-of-nisin-in-combination-with-p-coumaric-acid-against-foodborne-bacteria-bacillus-cereus-and-salmonella-typhimurium
#7
Anwesa Bag, Rabi Ranjan Chattopadhyay
The aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria Bacillus cereus and Salmonella typhimurium. Their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. Nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both the studied bacteria whereas nisin/ linalool combination showed synergistic activity against B...
August 16, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28815280/overcoming-challenges-in-single-particle-inductively-coupled-plasma-mass-spectrometry-measurement-of-silver-nanoparticles
#8
Jingyu Liu, Karen E Murphy, Michael R Winchester, Vincent A Hackley
Single particle ICP-MS has evolved rapidly as a quantitative method for determining nanoparticle size and number concentration at environmentally relevant exposure levels. Central to the application of spICP-MS is a commonly used, but not rigorously validated, calibration approach based on the measured transport efficiency and the response of ionic standards. In this work, we present a comprehensive and systematic study of the accuracy, precision and robustness of spICP-MS using the rigorously characterized reference material (RM) 8017 (Polyvinylpyrrolidone Coated Nominal 75 nm Silver Nanoparticles), recently issued by the National Institute of Standards and Technology (NIST)...
August 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28815239/-elimination-of-entercoccus-faecalis-with-different-disinfection-methods-in-root-canals-in-vitro
#9
Chu-Wen Sun, Ya-Qin Zhu
PURPOSE: To compare the elimination effect against E.faecalis in root canals with different methods. METHODS: Fifty extracted premolars with single root canal were selected. After cleaning and autoclaving, they were contaminated by E.faecalis and incubated for 28 days as models. Then the models were randomly and equally divided into 5 groups and treated as below: specimens in group A were treated with saline irrigation, specimens in group B were treated with 3% NaClO irrigation (as positive control), specimens in group C were treated with PUI, specimens in group D were treated with diode laser radiation, specimens in group E were treated with combination of PUI and diode laser radiation...
April 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/28814825/air-insufflated-high-definition-dacryoendoscopy-yields-significantly-better-image-quality-than-conventional-dacryoendoscopy
#10
Tsugihisa Sasaki, Tsutomu Sounou, Hideki Tsuji, Kazuhisa Sugiyama
PURPOSE: To facilitate the analysis of lacrimal conditions, we utilized high-definition dacryoendoscopy (HDD) and undertook observations with a pressure-controlled air-insufflation system. We report the safety and performance of HDD. METHODS: In this retrospective, non-randomized clinical trial, 46 patients (14 males and 32 females; age range 39-91 years; mean age ± SD 70.3±12.0 years) who had lacrimal disorders were examined with HDD and conventional dacryoendoscopy (CD)...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28814807/psychological-impact-of-vehicle-exhaust-exposure-insights-from-an-animal-model
#11
Ankita Salvi, Gaurav Patki, Hesong Liu, Samina Salim
Air pollution resulting from exhaust emissions of vehicles has risen in the recent years, reportedly causing major adverse effects on the heart, lungs and the brain. Though respiratory and cardiovascular effects of these emissions are well identified, psychological and neurobiological complications of prolonged exposure to vehicle emissions remain unknown. Pro-oxidants are considered as major constituents of vehicle emissions. This is important considering causal link between oxidative stress and behavioral and cognitive impairments...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28814094/the-role-of-pegaptanib-sodium-in-the-suppression-of-epidural-fibrosis-in-a-postlaminectomy-rat-model
#12
I Sertbas, A Yilmaz, T Yildirim, M Karatay, H Celik, M A Bayar
OBJECTIVE: Spinal epidural fibrosis is a clinical condition that develops after laminectomy and can compress the spine. Many agents have been tried for the treatment, but none has entered clinical use at present. Pegaptanib sodium is an antiangiogenetic drug that prevents the development of new vessels and thus adhesion by inhibiting the effect of VEGF. MATERIAL AND METHOD: 20 Wistar rats were used in this study. The rats were divided into 2 different groups as the control and pegaptanib sodium group...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/28813206/levamisole-in-illicit-trafficking-cocaine-seized-a-one-year-study
#13
Simona Martello, Massimo Pieri, Cristiano Ialongo, Stefano Pignalosa, Gianluca Noce, Francesca Vernich, Carmelo Russo, Federico Mineo, Sergio Bernardini, Luigi Tonino Marsella
Cocaine use is increasing around the world and its purity is frequently altered through dilution, substitution, contamination, and adulteration. Sugars, talc, starch, and carbonates represent the principal diluents of cocaine, while phenacetin, levamisole, caffeine, and lidocaine are its major adulterants in Europe. Levamisole is used because it is an odorless powder, with physical properties similar to cocaine, and it has reasonable cost and availability, being widely used in veterinary medicine. For this study, we analyzed 88 cocaine samples...
August 16, 2017: Journal of Psychoactive Drugs
https://www.readbyqxmd.com/read/28812090/zero-charged-catanionic-lamellar-liquid-crystals-doped-with-fullerene-c60-for-potential-applications-in-tribology
#14
Mengjun Chen, Baoyong Liu, Xiaolin Wang, Yanxu Fu, Jingcheng Hao, Hongguang Li
The formation of lamellar liquid crystals (LLCs) has been demonstrated in a few salt-free catanionic surfactant systems and their properties have been well documented. However, examples of their combination with other materials are relatively rare. Herein, a salt-free zero-charged catanionic surfactant with low chain melting temperature was prepared by mixing equimolar tetradecyltrimethylammonium oxide (TTAOH) and oleic acid (OA) in water, and its concentration-dependent aggregate transition was investigated...
August 16, 2017: Soft Matter
https://www.readbyqxmd.com/read/28811632/atomic-scale-study-of-the-amorphous-to-crystalline-phase-transition-mechanism-in-gete-thin-films
#15
R Mantovan, R Fallica, A Mokhles Gerami, T E Mølholt, C Wiemer, M Longo, H P Gunnlaugsson, K Johnston, H Masenda, D Naidoo, M Ncube, K Bharuth-Ram, M Fanciulli, H P Gislason, G Langouche, S Ólafsson, G Weyer
The underlying mechanism driving the structural amorphous-to-crystalline transition in Group VI chalcogenides is still a matter of debate even in the simplest GeTe system. We exploit the extreme sensitivity of (57)Fe emission Mössbauer spectroscopy, following dilute implantation of (57)Mn (T½ = 1.5 min) at ISOLDE/CERN, to study the electronic charge distribution in the immediate vicinity of the (57)Fe probe substituting Ge (FeGe), and to interrogate the local environment of FeGe over the amorphous-crystalline phase transition in GeTe thin films...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810774/effect-of-solute-nature-on-the-polyamorphic-transition-in-glassy-polyol-aqueous-solutions
#16
Yoshiharu Suzuki
I examined the polyamorphic behavior of glassy dilute aqueous solutions of polyols (ethylene glycol, glycerol, meso-erythritol, xylitol, and D-sorbitol) under pressure at low temperatures. Although the volume change of the glassy aqueous solution varied continuously against pressure, the rate of the volume change appeared to vary discontinuously at the onset pressure of the gradual polyamorphic transition. It is thought that low-density liquid-like solvent water and high-density liquid-like solvent water coexist during the transition...
August 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28810736/microstructure-of-flow-driven-suspension-of-hardspheres-in-cylindrical-confinement-a-dynamical-density-functional-theory-and-monte-carlo-study
#17
Hsiu-Yu Yu, Zahera Jabeen, David M Eckmann, Portonovo S Ayyaswamy, Ravi Radhakrishnan
We have studied the microstructure of a flow-driven hardsphere suspension inside a cylinder using dynamical density functional theory and Monte Carlo simulations. In order to be representative of various physical conditions that may prevail in experiments, we investigate the problem using both the grand canonical ($\mu VT$) ensemble and the canonical ($NVT$) ensemble. In both ensembles, the hydrodynamic effect on the suspension mediated by the presence of the confining wall is implemented in a mean-field fashion by incorporating the thermodynamic work done by the inertial lift force on the particle given the average flow field...
August 15, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28810179/utility-of-ultrasound-assisted-cloud-point-extraction-and-spectophotometry-as-a-preconcentration-and-determination-tool-for-the-sensitive-quantification-of-mercury-species-in-fish-samples
#18
Nail Altunay
The current study reports, for the first time, the development of a new analytical method employing ultrasound assisted-cloud point extraction (UA-CPE) for the extraction of CH3Hg(+) and Hg(2+) species from fish samples. Detection and quantification of mercury species were performed at 550nm by spectrophotometry. The analytical variables affecting complex formation and extraction efficiency were extensively evaluated and optimized by univariate method. Due to behave 14-fold more sensitive and selective of thiophene 2,5-dicarboxylic acid (H2TDC) to Hg(2+) ions than CH3Hg(+) in presence of mixed surfactant, Tween 20 and SDS at pH5...
August 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28809472/real-time-tracking-the-synthesis-and-degradation-of-albumin-in-complex-biological-systems-with-a-near-infrared-fluorescent-probe
#19
Qiang Jin, Lei Feng, Shui-Jun Zhang, Dan-Dan Wang, Fangjun Wang, Yi Zhang, Jing-Nan Cui, Wen-Zhi Guo, Guang-Bo Ge, Ling Yang
In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudo-esterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied with significant changes in both colour and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body...
August 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28808986/interaction-effects-in-assembly-of-magnetic-nanoparticles
#20
N A Usov, O N Serebryakova, V P Tarasov
A specific absorption rate of a dilute assembly of various random clusters of iron oxide nanoparticles in alternating magnetic field has been calculated using Landau-Lifshitz stochastic equation. This approach simultaneously takes into account both the presence of thermal fluctuations of the nanoparticle magnetic moments and magneto-dipole interaction between the nanoparticles of the clusters. It is shown that for usual 3D clusters, the intensity of the magneto-dipole interaction is determined mainly by the cluster packing density η = N p V/V cl , where N p is the average number of the particles in the cluster, V is the nanoparticle volume, and V cl is the cluster volume...
August 14, 2017: Nanoscale Research Letters
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